Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,620,067	A→G	Q155Q (CAA→CAG)	deoB →	phosphopentomutase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,620,067	0	A	G	93.3%	129.1 / ‑3.2	45	Q155Q (CAA→CAG)	deoB	phosphopentomutase
Reads supporting (aligned to +/- strand):  ref base A (1/2);  new base G (26/16);  total (27/18)
Fisher's exact test for biased strand distribution p-value = 5.55e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01

ACGGCAACGCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGCTAATCTGCCGGGTTACCTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAACTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCATGAAGAAACTTTCGGTCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAATATCGGTCGTGTTATCGCTCGTCCGTTTATCGGCGACAAAGCCGGTA‑ACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGC  >  NC_000913/4619832‑4620311                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                      aCGGAAACGCTGAAGTTATGGGCGTGTACGCATGGGCGCACTAAAGTTAATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATCGCCGTTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGCTAATCTGCCGGGTTACCTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAGCTGTGCGAAGAGcac                                                                                                                                                                                                                                        <  2:399987/251‑1 (MQ=255)         gCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATCGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGATAATCTGCCGGGTTACCTCGGTAACTGCCACTCTTCCGGTACGGTAATTCTGGATCAGCTGGGCGACGAGCACATGCAAAc                                                                                                                                                                                                                                >  2:456986/1‑251 (MQ=255)            gAAGTTTTCGGGGCGTACGCATGGGCGCACGAAATTTCACCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATCGCCGGTGTCCCGGTTCGGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTGCCCGCAAGAGCTGCGGGATAAACTGGTCGAACGCGCTAATCTGCCGGTTTACCCCGGTAACTGCCACTCTTCCGGTACGGGCATTCTGGATCAGCTGGGCGAAGAGCACATGAAAACCgg                                                                                                                                                                                                                             <  2:427992/251‑1 (MQ=255)                  aTCGGCGCGTACGCATGGGCGCAAGAAATGTCAACCGGTAAAGATCCCCCGTTTGGTCGCTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGTTAACCTGGTCGAACGCGCTAATCTGCCGGGTTCCCTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAACTGGGCGAAGAGCACATGAAAACCGGCAAGcc                                                                                                                                                                                                                       <  1:100589/251‑1 (MQ=255)                           tccccgccacgccgctcttcctaccccGGTAAAGATACCCCGTCTGGTCCCTGGGAAATCGCCGGTGTCCCGGTTCTGTTTGAGGGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCGGAATAAACTGGTCGAACGCGCTAATCTGCCGGTTTACCTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAGCTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCt                                                                                                                                                                                                               <  2:405868/225‑1 (MQ=255)                              gcaggggCGCAGGAAATGTCATCCGGGAAAGATACCCCGTGGGGGCTCTGGGAAATCGCCGGGGCCCCGGCGTGGTGTGAGGGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGGGCTGCTGGATAAACGGGGCGAACGCGCTAATCTGCGGGGTTACCGCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAGCTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATAc                                                                                                                                                                                                           <  1:425280/247‑1 (MQ=255)                              gCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATCGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGCTAATCTGCCGGGTTACCTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAGCTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATAc                                                                                                                                                                                                           <  1:124673/251‑1 (MQ=255)                               cATGGGCGCCCGAAATGTCATCCGTGAAAGATACCCCGTCGGGTCACTGGGAAATCGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCCCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGCTAATCTGCCGGGTTACCGCGGTAACTGCCACGCTGCCGGTACGGTCATTCTGGATCAGCTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATAcc                                                                                                                                                                                                          <  1:267866/251‑1 (MQ=255)                                                ccATCCGGTAAAGATCCCCCGTCGGCTCACTGGGAAATCGCCGGTGTCCCGTTTCTGTTTGAGGGGGGATATTTCTCCGATCACGAAAACAGCTCCCCGCAAGAGCTGCTGGATAAACTGGTCGACCGCGCTAATCTGCCGGGTTACCTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAGCTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGtt                                                                                                                                                                                         <  2:216776/250‑1 (MQ=255)                                                     cGGTAAAGATACCCCGTCTGGTCACTGGGAAATCGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGCTAATCTGCCGGGTTACCTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAGCTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCt                                                                                                                                                                                                               >  1:405868/1‑224 (MQ=255)                                                     cGGTAAAGATACCCCGTCTGGTCACTGGGAAATCGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGCTAATCTGCCGGGTTACCTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAGCTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTcc                                                                                                                                                                                       >  1:128947/1‑248 (MQ=255)                                                      gtaaaaGATTCCCCTTCTGTTCACGGGGAAATCGCGGGTGCCCCGGTTCTGTTTGAGGGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGACCTGCTGGATAAACTGGTCGAACGCGCTAATCTGCCGGGTTACCTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAGCTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGAt                                                                                                                                                                                   <  2:128947/248‑1 (MQ=255)                                                          aaGATACCCCGTCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAACCTGGTCGAACGTGCTAATCTGCCGGGTTACCTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAACTGGGTGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATtgcc                                                                                                                                                                               >  1:214355/1‑251 (MQ=255)                                                                        ggTCACTGGGAAATCGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGCTAATCTGCCGGGTTACCTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAGCTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCACGAAGGAAc                                                                                                                                                                 <  1:154759/251‑1 (MQ=255)                                                                                ggAAATCGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGCTAATCTGCCGGGTTACCTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAGCTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCACGAAGGAAcctttggcc                                                                                                                                                         >  2:46168/1‑243 (MQ=255)                                                                                         cGGTGTCCCGGTTCTGTTTGCGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGCTAATCTGCCGGGTTACCTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAGCTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCACGAAGGAACCTTTGGCCTGGATAAAc                                                                                                                                                >  2:260691/1‑251 (MQ=255)                                                                                         cGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGCTAATCTGCCGGGTTACCTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAGCTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCACGAAGGAACCTTTGGCCTGGATAAAc                                                                                                                                                >  1:136303/1‑251 (MQ=255)                                                                                                 cGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGCTAATCTGCCGGGTTACCTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAGCTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCACGCAGGAACCTTTGGCCTGGATAAACTCTACGa                                                                                                                                         >  1:83605/1‑250 (MQ=255)                                                                                                  ggTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGCTAATCTGCCGGGTTACCTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAGCTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCACGAAGGAACCTTTGGCCTGGATAAACTCTACGAAc                                                                                                                                       >  2:80353/1‑251 (MQ=255)                                                                                                  ggTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGCTAATCTGCCGGGTTACCTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAGCTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCAACGAAGGAACCTTTGGCCTGGATAAACTCTACGAAc                                                                                                                                       >  1:313040/1‑251 (MQ=255)                                                                                                        gTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGCTAATCTGCCGGGTTACCTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAGCTGGGCGAAGAGCACATGAAAACCGGCACGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCACGAAGGAACCTTTGGCCTGGCTAAACTCTACGAACTGTGCg                                                                                                                                 >  1:366428/1‑251 (MQ=255)                                                                                                                            ccGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACGGGTCGAACGCGCTAATCTGCCGGGTTCCCTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAGCTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCACGAAGGAACCTTTGGCCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGc                                                                                                               <  1:74481/249‑1 (MQ=255)                                                                                                                             cGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGCTAATCTGCCGGGTTACCTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAGCTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCCGACTCCGTGTTCCAGATTGCCTGCCACGAAGGAACCTTTGGCCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTg                                                                                                             >  1:408426/1‑250 (MQ=255)                                                                                                                                     aaagcAGCTTCCAGCAAGAGATGCTGGGTAAACTGGTCGAACGCGCTACTCTGCCGGGTTACCTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAGCTGGGCGAAGAGCACATGAAAACCGGCAAGACGATTTTCTATACCTCCGCTGACACCGTGTTCCAGATTGCCTGCCACGAAGGAACCTTTGGCCTGGATAAACTCTACGCACTGAGCGAAATCGCCCGTGAAGAGCTGACCACcggc                                                                                                    >  2:51825/5‑251 (MQ=255)                                                                                                                                         cAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGCTAATCTGCCGGGTTACCTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAGCTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCACGAAGGAACCTTTGGCCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGAACAAcggcggat                                                                                                >  1:454879/1‑249 (MQ=255)                                                                                                                                              tCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGCTAATCTGCCGGGTTACCTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAGCTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCACGAAGGAACCTTTGGCCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGACGGCCACaac                                                                                           >  1:185596/1‑250 (MQ=255)                                                                                                                                                      ggtctctgggaaaagagggtagAACGCGCTAACCTGCGGGGTTACCCGGGTAACTGCCACCCTTCCGGTACGGTCATTCTGGATCAGCTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCACGAAGGAACCTTTGGCCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCg                                                                                   <  2:14294/230‑1 (MQ=255)                                                                                                                                                             tgGATAAACTGGTCGAACGCGCTAATCTGCCGGGTTACCTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAGCTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCACGAAGGAACCTTTGGCCTGGATAAACTCTACGAACTGTGCGAAATCGCACGTGAAGAGCTGACCAAAggcggcaaacacaccgggcgtagtat                                                                             >  2:332951/1‑230 (MQ=255)                                                                                                                                                                  aaaCTGGTCGAACGCGCTAATCTGCCGGGTTACCTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAGCTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCACGAAGGAACCTTTGGCCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCGTGTTATCGCTCg                                                                       >  1:384306/1‑251 (MQ=255)                                                                                                                                                                           gAACGCGCTAATCTGCCGGGTTACCTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAGCTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCACGAAGGAACCTTTGGCCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCGTTTAt                                                              <  1:98987/251‑1 (MQ=255)                                                                                                                                                                              cgcgCTAATCTGCCGGGTTACCTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAGCTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCACGAAGGAACCTTTGGCCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCGTGGTATCGCTCGTCCGTTTATCgg                                                           >  1:46873/1‑251 (MQ=255)                                                                                                                                                                              ccgctaaacttGCCGGGTTACCTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAGCTGGGCGAAAAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATGGCCTGCCACGAAGGAACCTTTGGCCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCGTTTATCgg                                                           <  2:249613/241‑1 (MQ=255)                                                                                                                                                                                cgcTAATCTGCCGGGTTACCTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAGCTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCACGAAGGAACCTTTGGCCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCGTGTTATCGCCCGTCCGTTTATCggtg                                                         >  2:335448/1‑249 (MQ=255)                                                                                                                                                                                         gCCGGGTTACCTCGGTAACTGCCACTCTTCCGGAACGGACATTCTGGATCAGCTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCACGAAGGAACCTTTGGCCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTCCAAGATCGGTCGTGTTATCGCTCGTCCGTTTagaggtggcaaagccg                                                >  1:156041/1‑236 (MQ=255)                                                                                                                                                                                                    tCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAGCTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCACGAAGGAACCTTTGGCCTGGATAAACTCTACGAACTGTGCGAAATAGCCCGTGAAGAGCTGACCAACGGCGGCCACAACATCAGTCGTGGTATCGCTCGTCCGTTTTTGGGGGGCAAAGCCTGTA‑ACATCCa                                     >  2:83539/1‑250 (MQ=255)                                                                                                                                                                                                                      ccGGTACGGTCATTCTGGATCAGCTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCACGAAGGAACCTTTGGCCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTA‑ACTTCCAGCGTACCGGTAACCGTCAc                  >  1:323539/1‑251 (MQ=255)                                                                                                                                                                                                                         gTACGGTCATTCTGGATCAGCTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCACGAAGGAACCTTTGGCCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTA‑ACTTCCAGCGTACCGGTAACCGTCACggc               >  1:86987/1‑249 (MQ=255)                                                                                                                                                                                                                           aCGGTCATTCTGGATCAGCTGGGCGAAGAGCACATGAAAACCGGCAAGCCGAGTTTCTATCCCGCCGCTGACGCCGTGTTCCAGATTGCCTGCCACGAAGGAACCTTTGGCCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACAGCGGTCGTGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTA‑ACTTCCAGCGTACCGGTAACCGTCACGACCt             <  1:48344/251‑1 (MQ=255)                                                                                                                                                                                                                              gTCATTCTGGATCAGCTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCACGAAGGAACCTTTGGCCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTA‑ACTTCCAGCGTCCCGGAAACAGTCACGACCTGGc          >  1:296977/1‑251 (MQ=255)                                                                                                                                                                                                                              gTCATTCTGGATCAGCTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCACGAAGGAACCTTTGGCCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTAAAACATCGGGCGGGTTATGCCTCGTCCGGTTATTGGTGTCAAAACCGGTACATTTCC‑TCGTACCGGTGACCGTCGCGAcccgg           >  2:298971/1‑247 (MQ=255)                                                                                                                                                                                                                                cATTCTGGAGCAGCTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGGGTTCCAGATTGCCTGCCACGAAGGAACCTTTGGCCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTA‑ACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTg        <  2:194744/251‑1 (MQ=255)                                                                                                                                                                                                                                 aTTCTGGATCAACGGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATGCCGCCGCTGACTCCGTGTTCCAGATTGCCTGCCATGAAGAAACTTTCGGTCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAATATCGGTCGTGTTATCGCTCGTCCGTTTATCGGCGACAAAGCCGGTA‑ACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGt       <  1:12073/251‑1 (MQ=255)                                                                                                                                                                                                                                   tCTGGATCAGCTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCACGAAGGAACCTTTGGCCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAAACTCGGTCGTGTTATCACTCGTCCGTTTATCGGTGACAAAGCCGGTA‑ACTTCCAGCGTACAGGGAACCGTCACGACCTGGCTGttt     >  2:327556/1‑250 (MQ=255)                                                                                                                                                                                                                                     tGGATCAGCTGGGCGAAGAGCACATGTAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCACGAAGGAACCTTTGGCCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTA‑ACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGa    <  2:323539/250‑1 (MQ=255)                                                                                                                                                                                                                                      ggATCAGCTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCACGAAGGAACCTTTGGCCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACATTGGTCGTGTTATCGCTCGTCCGTTTTTCGGTGACAAAGCCGGTA‑ACTTCCAGCGTACCGGTAACCGTCACGACCTGGCGGTTTAgc  >  2:451566/1‑251 (MQ=255)                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                      ACGGCAACGCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGCTAATCTGCCGGGTTACCTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAACTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCATGAAGAAACTTTCGGTCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAATATCGGTCGTGTTATCGCTCGTCCGTTTATCGGCGACAAAGCCGGTA‑ACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGC  >  NC_000913/4619832‑4620311

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑