Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,619,917	T→C	I105I (ATT→ATC)	deoB →	phosphopentomutase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,619,917	0	T	C	95.7%	126.0 / ‑3.8	48	I105I (ATT→ATC)	deoB	phosphopentomutase
Reads supporting (aligned to +/- strand):  ref base T (1/1);  new base C (16/29);  total (18/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.52e-01

TCGGGGCTGACACCCTGGGTCATATCGCAGAAGCTTGTGCCAAAGGCGAAGCTGATAACGGTCGTAAAGGCCCGCTC‑AATCTGCCAAATCTGACCCGTCTGGGGCTGGCGAAAGCACACGAAGGTTCTACCGGTTTCATTCCGGCGGGAATGGACGGCAACGCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAG‑TGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGCTAATCTGCCGGGTTACCTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAACTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCATGAAGAAACTTTCGGTC  >  NC_000913/4619679‑4620161                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                      gcGGGGCTGACACCCTGGGTCATATCGCAGAAGCTTGTGCCAAAGGCGAAGCTGATAACGGTCGTAAAGGCCCGCTC‑AATCTGCCAAATCTGACCCGTCTGGGGCTGGCGAAAGCACACGAAGGTTCTACCGGTTTCATTCCGGCGGGAATGGACGGCAACGCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATCGCCGGTGTCCCg                                                                                                                                                                                                                                           <  2:6657/250‑1 (MQ=255)  cGGGGCTGACACCCTGGGTCATATCGCAGAAGCTTGTGCCAAAGGCGAAGCTGATAACGGTCGTAAAGGCCCGCTC‑AATCTGCCAAATCTGACCCGTCTGGGGCTGGCGAAAGCACACGAAGGTTCTACCGGTTTCATTCCGGCGGGAATGGACGGCAACGCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGGTACCCCGTCCTGTCACTGGGAAATCGCCGGTGTCCCgg                                                                                                                                                                                                                                          >  1:14294/1‑251 (MQ=255)      gCTGACACCCTGGGTCATATCGCAGAAGCTTGTGCCAAAGGCGAAGCTGATAACGGTCGTAAAGGCCCGCTC‑AATCTGCCAAAGCTGACCCGTCTGGGGCTGGCGAAAGCACACGAAGGTTCTACCGGTTTCATTCCGGCGGGAATGGACGGCAACGCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATCGCCGGTGTCCCGGTTCt                                                                                                                                                                                                                                      <  1:209852/251‑1 (MQ=255)         gACACCCTGGGTCATATCGCAGAAGCTTGTGCCAAAGGCGAAGCTGATAACGGTCGTAAAGGCCCGCTC‑AATCTGCCAAATCTGACCCGTCTGGGGCTGGCGAAAGCACACGAAGGTTCTACCGGTTTCATTCCGGCGGGAATGGACGGCAACGCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATCGCCGGTGTCCCGGTTCTGt                                                                                                                                                                                                                                    <  1:215001/250‑1 (MQ=255)         gACACCCTGGGTCATATCGCAGAAGCTTGTGCCAAAGGCGAAGCTGATAACGGTCGTAAAGGCCCGCTC‑AATCTGCCAAATCTGACCCGTCTGGGGCTGGCGAAAGCACACGAAGGTTCTACCGGTTTCAGTCCGGCGGGAATGGACGGCAACGCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATCGCCGGTGTCCCGGTTCTGt                                                                                                                                                                                                                                    <  2:242568/250‑1 (MQ=255)          acccccTGGGTCATATCGCAAAAGCTTGGGCCAAAGGCGCAGCTGATAACGGTCGTAAAGGCCC‑CTCCAACCTGCCAAATCTGACCCGTCTGGGGCTGGCGAAAGCACACGAAGGTTCCACCGGTTTCATTCCGGCGGGAATGGACGGCAACGCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATCGCCGGTGTCCCGGTGCTgggt                                                                                                                                                                                                                                  <  2:351501/248‑4 (MQ=255)              ccTGGGTCATATCGCAGAAGCTTGTGCCAAAGGCGAAGCTGATAACGGGCGTAAAGGCCCGCTC‑AATCTGCCAAATCTGACCCGTCTGGGGCTGGCGAAAGCACACGAAGGTTCTACCGGTTTCATTCCGGCGGGAATGGACGGCAACGCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCAGCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATCGCCGGTGTCCCGGTTCTGTTTGAG‑t                                                                                                                                                                                                                             <  2:375919/251‑1 (MQ=255)                              gAAGCTTGTGCCAAAGGCGAAGCTGATAACGGTCGTAAAGGCCCGCTC‑AATCTGCCAAATCTGACCCGTCTGGGGCTGGCGAAAGCACACGAAGGTTCTACCGGTTTCATTCCGGCGGGAATGGACGGCAACGCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAACTCGCCGGTGTCCCGGTTCTGTTTGCG‑GGGGGATCTTTCTCCgg                                                                                                                                                                                                             >  2:154759/1‑250 (MQ=255)                                  cTTGTGCCAAAGGCGAAGCTGATAACGGTCGTAAAGGCCCGCTC‑AATCTGCCAAATCTGACCCGTCTGGGGCTGGCGAAAGCACACGAAGGTTCTACCGGTTTCATTCCGGCGGGAATGGACGGCAACGCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGTAATCCGGGAAAGATACCCCGTCGGGTCACTGGGAAATCGCCGGTGTCCCGGTTCTGTTTGAG‑GGGGGGTATTTCTCCGATccc                                                                                                                                                                                                         >  1:434737/1‑249 (MQ=255)                                    tgtgCCAAAGGCGAAGCTGATAACGGTCGTAAAGGCCCGCTC‑AATCTGCCAAATCTGACCCGTCTGGGGCTGGCGAAAGCACACGAAGGTTCTACCGGTTTCATTCCGGCGGGAATGGACGGCAACGCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATCGCCGGTGTCCCGGTTCTGTTTGAG‑TGGGGATATTTCTCCGATCAc                                                                                                                                                                                                         <  1:446387/249‑1 (MQ=255)                                     gtgCCAAAGGCGAAGCTGATAACGGTCGTAAAGGCCCGCTC‑AATCTGCCAAATCTGACCCGTCTGGGGCTGGCGAAAGCACACGAAGGTTCTACCGGTTTCATTCCGGCGGGAATGGCCGGCAACGCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATAGCCGGTGTCCCGGTTCTGTTTGAG‑GGGGGCTATTTCTCCGATCACgg                                                                                                                                                                                                       >  1:194744/1‑249 (MQ=255)                                      tgCCAAAGGCGAAGCTGATAACGGTCGTAAAGGCCCGCTC‑AATCTGCCAAATCGGACCCGTCTGGGGCTGGCGAAAGCACACGAAGGTTCTACCGGTTTCATTCCGGCGGGAATGGACGGCAACGCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATCGCCGGTGTCCCGGTTCTGTTTGa                                                                                                                                                                                                                                <  1:297024/225‑1 (MQ=255)                                        ccAAAGGCGAAGCGGATAACGTTCGTAAAGGCCCGCTC‑AATCTGCCAAAGCTGACCCGTCTGGGGCTGGCGAAAGCACACGAAGGTTCTACCGGTTTCATTCCGGCGGGAATGGACGGCAACGCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATCGCCGGTGTCCCGGTTCTGTTTGAG‑TGGGGATATTTCTCCGATCACGAAAAc                                                                                                                                                                                                   <  2:117025/251‑1 (MQ=255)                                         cAAAGGCGAAGCTGATAACGGTCGTAAAGGCCCGCTC‑AATCTGCCAAATCTGACCCGTCTGGGGCTGGCGAAAGCACACGAAGGTTCTACCGGTTTCATTCCGGCGGGAATGGACGGCAACGCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGCAATCGCCGGGGTCCCGGTTCTGTTTGAG‑TGGGGATATTTCTCCGCTCACGAAAAc                                                                                                                                                                                                   >  1:60958/1‑250 (MQ=255)                                             gtggAAGCTGATAACGGTCGGAAAGGCCCGCGC‑AATCTGCCAAAGCTGACCCGTCTGGGGCTGGCGAAAGCACACGAAGGTTCTACCGGTTTCATTCCGGCGGGAATGGACGGCAACGCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGGCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATCGCCGGTGTCCCGGTTCTGTTTGAG‑TGGGTATATTTCTCCGATCACGAAAACAGCtt                                                                                                                                                                                              <  2:64961/248‑1 (MQ=255)                                             ggCGAAGCTGAGAACGGTCGTAAAGGCCCGCTC‑AATCTGCCAAATCTGACCCGTCTGGGGCTGGCGAAAGCACACGAAGGGTCTACCGGTTTCATTCCGGCGGGAATGGACGGCAACGCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATCGCCGGGGTCCCGGTTCTGTTTGAG‑TGGGGATAGTTCTCCGATCACGAAAACAGCtt                                                                                                                                                                                              <  2:60958/251‑1 (MQ=255)                                                gAAGCTGATAACGTTCGTAAAGGCCCGCTC‑AATCTGCCAAATCTGACCCGTCTGGGGCTGGCGAAAGCACACGAAGGTTCTACCGGTTTCATTCCGGCGGGAATGGACGGCAACGCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATCGCCGGTGTCCCGGTTCTGTTTGAG‑TGGGGATATTTCTCCGATCACGAAAACAGCTTccc                                                                                                                                                                                           <  1:30340/251‑1 (MQ=255)                                                gAAGCTGATAACGGTCGTAAAGGCCCGCTC‑AATCTGCCAAATCTGACCCGTCTGGGGCTGGCGAAAGCACACGAAGGTTCTACCGGTTTCATTCCGGCGGGAATGGACGGCAACGCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATCGCCGGTGTCCCGGTTCTGTTTGAG‑TGGGGATATTTCTCCGATCACGAAAACAGCTTccc                                                                                                                                                                                           >  2:124673/1‑251 (MQ=255)                                                      gATAACGGTCGTAAAGGCACGCTA‑AATCTGGCAAAGCTGACCCGTCTGGGGCTGGCGAAAGCACACGAAGGTTCTACCGGTTTCATTCCGGCGGGAATGGACGGCAACGCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATCGCCGGTGTCCCGGTTCTGTTTGAG‑TGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAaga                                                                                                                                                                                     <  2:10128/251‑1 (MQ=255)                                                      gATAACGGGCGTAAAGGCCCGCTC‑AATCGGCCAAATCTGACCCGTCTGGGGCTGGCGAAAGCACACGAAGGTTCTACCGGGTTCAGTCCGGCGGGAATGGACGGCAACGCTGAAGTTAGCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATTCCCCGTCTGGTCACTGGGAAATCGCCGGTGTCCCGGTTCTGTTTGAG‑TGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAag                                                                                                                                                                                      <  2:80098/250‑1 (MQ=255)                                                                  aaaGGCCCGCTC‑AATCTGCCAAATCTGACCCGTCTGGGGCTGGCGAAAGCACACGAAGGTTCTACCGGTTTCATTCCGGCGGGAATGGACGGCAACGCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATCGCCGGTGTCCCGGTTCTGTTTGAG‑TGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATaa                                                                                                                                                                         <  1:379661/251‑1 (MQ=255)                                                                  aaaGGCCCGCTC‑AATCTGCCAAATCTGACCCGTCTGGGGCTGGCGAAAGCACACGAAGGTTCTACCGGTTTCATTCCGGCGGGAATGGACGGCAACGCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATCGCCGGTGTCCCGGTTCTGTTTGAG‑TGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATa                                                                                                                                                                          <  1:172240/250‑1 (MQ=255)                                                                  aaaGGCCCGCTC‑AATCTGCCAAATCTGACCCGTCTGGGGCTGGCGAAAGCACACGAAGGTTCTACCGGTTTCATTCCGGCGGGAATGGACGGCAACGCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATCGCCGGTGTCCCGGTTCTGTTTGAG‑TGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATa                                                                                                                                                                          >  1:12085/1‑250 (MQ=255)                                                                    aGGCCCGCTC‑AATCTGCCAAATCTGACCCGTCTGGGGCTGGCGAAAGCACACGAAGGTTCTACCGGTTTCATTCCGGCGGGCATGGACGGCAACGCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATCGCCGGTGTCCCGGTTCTGTTTGAG‑TGGGGATATTTCTCCGATCACGCAAACAGCTTCCCGCAAGAGCTGCTGGATAAAc                                                                                                                                                                       >  1:399987/1‑251 (MQ=255)                                                                      gCCCGCTC‑AATCTTCCAAATCAGACCCGTCTGGGGCTGGCGAAAGCACACGAAGGTTCGACCGGTTTCATTCCGGCGGGAATGGACGGCAACGCTGAAGTGATCGTCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATCGCCGGTGTCCCGGTTCTGTTTGAG‑TGGGTATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTg                                                                                                                                                                     <  1:458563/251‑1 (MQ=255)                                                                          gCTC‑AATCTGCCAAATCTGACCCGTCTGGGGCTGGCGAAAGCACACGAAGGTTCTACCGGTTTCATTCCGGCGGGAATGGACGGCAACGCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATCGCCGGTGTCCCGGTTCTGTTTGAG‑TGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCg                                                                                                                                                                 <  2:152265/251‑1 (MQ=255)                                                                             c‑AATCTGCCAAATCTGACCCGTCTGGGGCTGGCGCAAGCACACGAAGGTTCTACCGGTTTCATTCCGGCGGGAATGGACGGCAACGCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATCGCCGGTGTCCCGGTTCTGTTTGAG‑TGGGGATATTTCTCCGATTCCGAAAACAGCTTCCCGCCAGAGCTGCTGGATAAACTGGGCGAAc                                                                                                                                                              >  1:146232/1‑251 (MQ=255)                                                                                     ccAAATCTGACCCGTCTGGGGCTGGCGAAAGCACACGAAGGTTCTACCGGTTTCATTCCGGCGGGGATGGACGGCAACGCTGAAGTTATCGGCGCGTACGCATGGGCGCGCGAAATGTCCTCCGGTAAAGATACCCCGTCTGGGACCTGGGAGATCGCCGGTGTCCCAGTTATGTTTGAGGTGGGG‑TATTTCTCCGCTCCCGGAAACAGCTTCCCCCACGAGCTGCTGGATaacctctacgaacccgcca                                                                                                                                                        >  2:425280/1‑233 (MQ=255)                                                                                      cAAATCTGACCCGTCTGGGGCTGGCGAAAGCACACGAAGGTTCTACCGGTTTCATTCCGGCGGGAATGGACGGCAACGCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATCGCCGGTGTCCCGGTTCTGTTTGAG‑TGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGCTAAt                                                                                                                                                      >  1:216776/1‑251 (MQ=255)                                                                                             gACCCGTCTGGGGCTTGCGAAGGCACACGAAGGTTCTACCGGTATCATTCCGGCGGGAATGGAAGGCAACGCTGAAGTTAGCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAAGCGCCGGTGTCCCGGTTCTGGTTGAG‑TGGGGATATGTCTCCGATCACGAAAACAGCTTCCCGCAAGAGGTGCTGGATAAACTGGTCGAACGCGCTAATCTGCCg                                                                                                                                                <  2:242313/250‑1 (MQ=255)                                                                                             gACCCGTCTGGGGCTGGCGAAAGCACACGAAGGTTCTACCGGTTTCATTCCGGCGGGAATGGACGGCAACGCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATCGCCGGTGTCCCGGTTCTGTTTGAG‑TGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGCTAATCTGcc                                                                                                                                                 <  1:93592/249‑1 (MQ=255)                                                                                                                       acGAAGGTTCTACCGGTTTCATTCCGGCGGGAATGGACGGCAACGCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATCGCCGGTGTCCCGGTTCTGTTTGAG‑TGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGCTAATCTGCCGGGTTACCTCGGTAACTGCCACTCTTcc                                                                                                                     >  2:98987/1‑251 (MQ=255)                                                                                                                                    cGGTTTCATTCCGGCGGGAATGGACGGCAACGCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATCGCCGGTGTCCCGGGTCTGTTTTAG‑TGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGCTAATCTGCCGGGTTACCTCGGTAACTGCCACTCTTCCGGTACGGTCATTc                                                                                                        >  1:100455/1‑251 (MQ=255)                                                                                                                                                           aCGGAAACGCTGAAGTTATGGGCGTGTACGCATGGGCGCACTAAAGTTAATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATCGCCGTTGTCCCGGTTCTGTTTGAG‑TGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGCTAATCTGCCGGGTTACCTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAGCTGTGCGAAGAGcac                                                                                 <  2:399987/251‑1 (MQ=255)                                                                                                                                                                   gCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATCGCCGGTGTCCCGGTTCTGTTTGAG‑TGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGATAATCTGCCGGGTTACCTCGGTAACTGCCACTCTTCCGGTACGGTAATTCTGGATCAGCTGGGCGACGAGCACATGCAAAc                                                                         >  2:456986/1‑251 (MQ=255)                                                                                                                                                                      gAAGTTTTCGGGGCGTACGCATGGGCGCACGAAATTTCACCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATCGCCGGTGTCCCGGTTCGGTTTGAG‑TGGGGATATTTCTCCGATCACGAAAACAGCTGCCCGCAAGAGCTGCGGGATAAACTGGTCGAACGCGCTAATCTGCCGGTTTACCCCGGTAACTGCCACTCTTCCGGTACGGGCATTCTGGATCAGCTGGGCGAAGAGCACATGAAAACCgg                                                                      <  2:427992/251‑1 (MQ=255)                                                                                                                                                                            aTCGGCGCGTACGCATGGGCGCAAGAAATGTCAACCGGTAAAGATCCCCCGTTTGGTCGCTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAG‑TGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGTTAACCTGGTCGAACGCGCTAATCTGCCGGGTTCCCTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAACTGGGCGAAGAGCACATGAAAACCGGCAAGcc                                                                <  1:100589/251‑1 (MQ=255)                                                                                                                                                                                     tccccgccacgccgctcttcctaccccGGTAAAGATACCCCGTCTGGTCCCTGGGAAATCGCCGGTGTCCCGGTTCTGTTTGAG‑GGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCGGAATAAACTGGTCGAACGCGCTAATCTGCCGGTTTACCTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAGCTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCt                                                        <  2:405868/225‑1 (MQ=255)                                                                                                                                                                                        gcaggggCGCAGGAAATGTCATCCGGGAAAGATACCCCGTGGGGGCTCTGGGAAATCGCCGGGGCCCCGGCGTGGTGTGAG‑GGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGGGCTGCTGGATAAACGGGGCGAACGCGCTAATCTGCGGGGTTACCGCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAGCTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATAc                                                    <  1:425280/247‑1 (MQ=255)                                                                                                                                                                                        gCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATCGCCGGTGTCCCGGTTCTGTTTGAG‑TGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGCTAATCTGCCGGGTTACCTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAGCTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATAc                                                    <  1:124673/251‑1 (MQ=255)                                                                                                                                                                                         cATGGGCGCCCGAAATGTCATCCGTGAAAGATACCCCGTCGGGTCACTGGGAAATCGCCGGTGTCCCGGTTCTGTTTGAG‑TGGGGATATTTCCCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGCTAATCTGCCGGGTTACCGCGGTAACTGCCACGCTGCCGGTACGGTCATTCTGGATCAGCTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATAcc                                                   <  1:267866/251‑1 (MQ=255)                                                                                                                                                                                                          ccATCCGGTAAAGATCCCCCGTCGGCTCACTGGGAAATCGCCGGTGTCCCGTTTCTGTTTGAG‑GGGGGATATTTCTCCGATCACGAAAACAGCTCCCCGCAAGAGCTGCTGGATAAACTGGTCGACCGCGCTAATCTGCCGGGTTACCTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAGCTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGtt                                  <  2:216776/250‑1 (MQ=255)                                                                                                                                                                                                               cGGTAAAGATACCCCGTCTGGTCACTGGGAAATCGCCGGTGTCCCGGTTCTGTTTGAG‑TGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGCTAATCTGCCGGGTTACCTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAGCTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCt                                                        >  1:405868/1‑224 (MQ=255)                                                                                                                                                                                                               cGGTAAAGATACCCCGTCTGGTCACTGGGAAATCGCCGGTGTCCCGGTTCTGTTTGAG‑TGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGCTAATCTGCCGGGTTACCTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAGCTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTcc                                >  1:128947/1‑248 (MQ=255)                                                                                                                                                                                                                gtaaaaGATTCCCCTTCTGTTCACGGGGAAATCGCGGGTGCCCCGGTTCTGTTTGAG‑GGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGACCTGCTGGATAAACTGGTCGAACGCGCTAATCTGCCGGGTTACCTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAGCTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGAt                            <  2:128947/248‑1 (MQ=255)                                                                                                                                                                                                                    aaGATACCCCGTCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAG‑TGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAACCTGGTCGAACGTGCTAATCTGCCGGGTTACCTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAACTGGGTGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATtgcc                        >  1:214355/1‑251 (MQ=255)                                                                                                                                                                                                                                  ggTCACTGGGAAATCGCCGGTGTCCCGGTTCTGTTTGAG‑TGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGCTAATCTGCCGGGTTACCTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAGCTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCACGAAGGAAc          <  1:154759/251‑1 (MQ=255)                                                                                                                                                                                                                                          ggAAATCGCCGGTGTCCCGGTTCTGTTTGAG‑TGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGCTAATCTGCCGGGTTACCTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAGCTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCACGAAGGAAcctttggcc  >  2:46168/1‑243 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                      TCGGGGCTGACACCCTGGGTCATATCGCAGAAGCTTGTGCCAAAGGCGAAGCTGATAACGGTCGTAAAGGCCCGCTC‑AATCTGCCAAATCTGACCCGTCTGGGGCTGGCGAAAGCACACGAAGGTTCTACCGGTTTCATTCCGGCGGGAATGGACGGCAACGCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAG‑TGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGCTAATCTGCCGGGTTACCTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAACTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCATGAAGAAACTTTCGGTC  >  NC_000913/4619679‑4620161

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑