Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,619,197	G→A	P323P (CCG→CCA)	deoA →	thymidine phosphorylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,619,197	0	G	A	96.4%	174.0 / NA	57	P323P (CCG→CCA)	deoA	thymidine phosphorylase
Reads supporting (aligned to +/- strand):  ref base G (0/1);  major base A (28/26);  minor base C (1/1);  total (29/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACATGAATCAGGTACTGGCCTCCAGTGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGCAGTTCCTGACGGGTGAATATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTGATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTGCAGGCGGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAGCGGCACAAAAAGGCCCGACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTGCAATGGGCGGCGGACGCCGTCAGGCATCTGACACCATCGATTACAGCGTCGGCTTTACTGATATGGCGCGTCTGGGCGACCAGGTAGACGGTCAGCGTCCGCTGGCGGTTATCCACGC  >  NC_000913/4618950‑4619439                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                   aCATGAATCGGGTACTGGCCTCCATGGCAGGTAACGCGGTGGAAGTTCGTGAAGCGGTGCAGTTCCTGACGGGTGAGTATCGTATCCCGCTGCTGTTTGATGTCACGATGGCGCCGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTGCAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAGCGGCACAAAAAGGCccaacc                                                                                                                                                                                                                                                 <  2:469309/251‑1 (MQ=255) aCATGAATCAGGTACTGGCCTCCAGTGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGCAGTTCCTGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTGCAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAGCGGCACAAAAAGGCccaacc                                                                                                                                                                                                                                                 <  1:355513/251‑1 (MQ=255)   aTGAATCAGGTACTGGCCTCCAGTGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGCAGTTCCTGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGACGCGCGCGCGAAATTGCAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGGCTTTGGTCGTATGGTAGCGGCACACAAAGGCccaaccg                                                                                                                                                                                                                                                >  2:407745/1‑250 (MQ=255)       aTCAGGTACTGGCCTCCAGTGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGCAGGTCCTGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTGCAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAGCGGCACAAAAAGGCCCAACCGACTTc                                                                                                                                                                                                                                           <  2:10154/251‑1 (MQ=255)         cAGGTTCTGGCCGCCAGTGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGCAGTTCCTGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCACCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTGCAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCGGTGGTCGTATGGTAGCGGCACAAAAAGGACCCACCGACTTCg                                                                                                                                                                                                                                          <  2:59595/250‑1 (MQ=255)                    cTCCAGTGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGCAGTTCCTGGCGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGAGGAGATGCTTCTCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTGCAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAGCGGCACAAAACGGCCCAACCGCCTTCGTTGAGAACTAcg                                                                                                                                                                                                                              >  2:81056/1‑251 (MQ=255)                      ccAGTGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGCAGTTCCTGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTGCAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAGCGGCACAAAAAGGCCCAACCGACTTCGTTGAGAACTAcgcg                                                                                                                                                                                                                            >  1:1083/1‑251 (MQ=255)                          tGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGCAGTTCCTGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTGCAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAGCGGCACAAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGt                                                                                                                                                                                                                        >  1:6987/1‑251 (MQ=255)                          ggtagggaacgcggtgtaattgcgggAAGCGGTGCTTTTCCCGACGGGGGAGTATCGTAACCCGCGTCTTTTTGATGTCACGATGGCGCTGGGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTGCAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAGCGGCACAAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGt                                                                                                                                                                                                                        <  2:399139/226‑1 (MQ=255)                            cAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGCAGTTCCTGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTGCAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAGCGGCACAAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTAt                                                                                                                                                                                                                      >  1:478238/1‑251 (MQ=255)                             aGGTAACGCGGTTGAAGTTCGTGAAGCGGTGCAGTTCCTGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTGCAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAGCGGCACAAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATc                                                                                                                                                                                                                     >  1:435103/1‑251 (MQ=255)                              ggTAACGCGGTTGAAGTTCGTGAAGCGGTGCAGTTCCTGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTGCAAGCAGTGCTGGACAACGGAAAAGCGGCAGAAGTCTTTGGTCGTATGGTAGCGGCACAAACAGGCCCCACCGACTTCGTTGCGAACTACGCGAGGTATCt                                                                                                                                                                                                                    >  2:267944/1‑251 (MQ=255)                               gTAACGCGGTTGAAGTTCGTGAAGCGGTGCAGTTCCTGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTGCAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAGCGGCACAAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCTg                                                                                                                                                                                                                   <  2:24726/251‑1 (MQ=255)                                 aaCGCGGTTGAAGTTCGTGAAGCGGTGCAGTTCCTGACAGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTGCAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAGCGGCACAAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCTGcc                                                                                                                                                                                                                 >  1:90088/1‑251 (MQ=255)                                     gggTTGAAGTTCTGGAAGCGGTGCAGTTCCTGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTGCAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAGCGGCACAAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACa                                                                                                                                                                                                             <  2:359745/250‑1 (MQ=255)                                             gTTCGTGAAGCGGTGCAGTTCCTGACGTGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTGCAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAGCGGCACAAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCt                                                                                                                                                                                                     <  2:331050/251‑1 (MQ=255)                                             gTTCGTGAAGCGGTGCAGTTCCTGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTGCAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAGCGGCACAAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCt                                                                                                                                                                                                     >  1:331050/1‑251 (MQ=255)                                             gTTCGTGAAGCGGTGCAGTTCCTGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTGCAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAGCGGCACAAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCt                                                                                                                                                                                                     <  2:155417/251‑1 (MQ=255)                                                cGTGAAGCGGTGCAGTTCCTGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTGCAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAGCGGCACAAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGAc                                                                                                                                                                                                  >  1:108423/1‑251 (MQ=255)                                                 atGAAGCGGTGCAGTTCCTGACGGGTGAATATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTGATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTGCAGGCGGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAGCGGCACAAAAAGGCCCGACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACg                                                                                                                                                                                                 <  2:98242/250‑1 (MQ=255)                                                    aaGCGGTGCAGTTCCTGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTGCAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAGCGGCCCAAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGCAGTATCTGCCGACAGCGATGCTGACGaa                                                                                                                                                                                               >  2:248331/1‑250 (MQ=255)                                                               ttCCTGACGGGTGAGTATCGTAACCCGCGTCTGTTTGCTGTCACGATGGCGCTGGGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTGCAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAGCGGCACAAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGCCGAAAGAAGTCTATGc                                                                                                                                                                                   >  2:483270/1‑251 (MQ=255)                                                                   tGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTGCAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAGCGGCACAAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGAt                                                                                                                                                                               >  1:280967/1‑251 (MQ=255)                                                                    gACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTGCAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAGCGGCACAAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGa                                                                                                                                                                                <  1:234984/249‑1 (MQ=255)                                                                    gACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTGCAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAGCGGCACAAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATa                                                                                                                                                                              <  2:196691/251‑1 (MQ=255)                                                                            agtatCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTGCACGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAGCGGCACAAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACAAAAGCAGTCTATGCTGATACCGAAGGt                                                                                                                                                                      >  2:325780/3‑251 (MQ=255)                                                                                       ccGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTGCAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAGCGGCACAAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTg                                                                                                                                                            >  1:244998/1‑250 (MQ=255)                                                                                            gcTGTTTGATGTCACGATGGCTCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGAGGACGCCGAAGCGCGCGCGAAATTGCAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAGCGGCACAAAAAGTCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATgg                                                                                                                                                      <  2:424693/250‑1 (MQ=255)                                                                                              tGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAACGATGACGCCGAAGCGCGCGCGAAATTGCAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAGCGGCACAAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGCTACCGAAGGTTTTGTCAGTGCAATGGAt                                                                                                                                                    >  1:64961/1‑251 (MQ=255)                                                                                               gttggaggTCACGAGGGGGCTGTGCGTGGAGAGGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGTAATTGCAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAGCGGCACAAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATa                                                                                                                                                   <  2:112292/244‑1 (MQ=255)                                                                                               gTTTGATTGCACGATGGCGCGGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTGCAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAGCGGCACAAAAAGGCACAACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGAt                                                                                                                                                    <  2:122097/250‑1 (MQ=255)                                                                                               gTTTGATGTCACGATGGCGCTGTTCGTGGAGATGCTTATCTCCGTCAAACTGGTGAAAGATGACGCCGAAGCGCGCGCGAAATTGCAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAGCGGCACAAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGAt                                                                                                                                                    <  2:1083/250‑1 (MQ=255)                                                                                                 ttGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTGCAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAGCGGCACAAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATAcc                                                                                                                                                 >  1:4898/1‑251 (MQ=255)                                                                                                           gATGGCGCTGTGCGTGGAGATGCTTATCTCCGGAAAACTGGCGCAAGATGACGCCGAAGCGCGCGCGAAATTGCAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAGCGGCACAAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATACTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTgg                                                                                                                                       >  1:131448/1‑251 (MQ=255)                                                                                                                cgcTGTGCGCTGAGCTGCTTATCTCAGGCAAACCGGCGAAAGATGGCGCCGACGCGCGAGCGACATTGCAAGCAGTGCTGGACACCGGTAAAGCGGCAGAAGTCTTTGGTCGTATCGTACCGGCACAAAAAGGCCCAACCGCCTTCGTTGAGAATTGAGCGAAGTATCTGCCGTCCGCGCTGCTGACGAAAGAAGACAATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCAGCGCGCTggggttg                                                                                                                                  >  2:236244/1‑248 (MQ=255)                                                                                                                  cTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTGCAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAGCGGCACAAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGGGATGGc                                                                                                                                >  1:162842/1‑251 (MQ=255)                                                                                                                          ggAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTGCAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAGCGGCACAAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTg                                                                                                                        <  1:105605/251‑1 (MQ=255)                                                                                                                          ggAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTGCAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAGCGGCACAAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGAGGTTg                                                                                                                        >  2:408159/1‑251 (MQ=255)                                                                                                                                tcttaTCTCCGGCAAACTGGCAAAAGAGGACGCGGAAGCGCGCGCGAAAGTGCAAGCCGTGCGGGACAACGGTAAAGCGGCAGAAGGCTTTGGTCGTATGGTAGCGGCACAAAAAGGCCCAACCGACTGCGTTGAGAACTACGCGAAGTATCTGCGGACACCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTGCAATg                                                                                                                   <  1:456293/246‑1 (MQ=255)                                                                                                                                gcttaTCTCCGGCAAACTGGCGAAAGATGACGCCTAAGCGCGCGCGAAATTGCAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAGCGGCACAAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTGCAATgg                                                                                                                  <  1:269028/247‑1 (MQ=255)                                                                                                                                   taTCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAAGTGCAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAGCGGCACAAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGGCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTGCAATGggc                                                                                                                <  1:267944/249‑1 (MQ=255)                                                                                                                                        ccGGCAAAAGGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTGCAAGCAGGGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTTGCGGCACAAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAAGGGATACCCGCGCGCTGGGGATGGCAGTGGTTTCAATGggcggc                                                                                                             <  2:70522/248‑1 (MQ=255)                                                                                                                                          ggCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTGCAAGAAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAGCGGCAAAAAAAGGCCCAACCGACTTCGTGGAGAACTACGCGAAGAATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGAAACCGAAGGTTTTGTAAGGGAAATGGATACACGCGAGCTGGGGTGGGCAGAGGTAGCAATGGGGGGCGGACg                                                                                                        >  2:424745/1‑251 (MQ=255)                                                                                                                                             aaaCTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTGCAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAGCGGCACAAAAAGGCCCAACCGACTTCGTTGAGCACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGGGTTGGCCGGGGTTGCATTTGGcggcgggggtcg                                                                                                     >  2:264142/1‑245 (MQ=255)                                                                                                                                              aaCTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTGCAAGCAGGGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAGCGGCACAAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTGCAATGGGcggcggtcgtcgt                                                                                                    <  2:108423/251‑8 (MQ=255)                                                                                                                                                    ggAAAGACGACGACGAAGCGCTCGGGAAAGTGCAAGCAGTGCTGGACAACGGTAAAGCGGAAGAAGTCGTTGGTCGGATGGTAGCGGCACAAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGGCTAGGCTGATACCGAATGTTTTGTCAGTGAAATGGATACCCGCGCGCCGTGGATGTCAGTGGTCGCAATGTGCGGCGGTCGTCGTCAGGCa                                                                                              <  2:71509/250‑1 (MQ=255)                                                                                                                                                       aaGATGACGCCGAAGCGCGCGCGAAATTGCAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAGCGGCACAAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTGCAATGGGCGGCGGTCGTCGTCAGGCATCt                                                                                           <  1:365441/251‑1 (MQ=255)                                                                                                                                                                    aGCGCGCGCAAAATTGCAAGCTATTCTGGACAACGGTAAAGTGGCACAAGTCTGTGGTCGTATGGTATCGGCTCAAAAAGGCCTAACCGACTGCGTTGAGAACCACGCGACGTATCTGCCGACAGCGATGCGGACGAAAGCAGTCTATGCTTATCCCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTGCAATGGGCGGCGGTCGTCGTCAGGCATCTGACACCATTGAtt                                                                              <  2:111905/251‑1 (MQ=255)                                                                                                                                                                        cgcgcgAAATTGCAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAGCGGCACAAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTGCAATGGGCGGCGGTCGTCGTCAGGCATCTGACACCATTGATTACAg                                                                          <  2:478238/251‑1 (MQ=255)                                                                                                                                                                                aTTGCAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAGCGGCACAAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGATGGGGATGGCAGTGGTTGCAATGGGCGGCGGTCGTCGTCAGGCATCTGACACCATTGATTAAAGCGTCGGCt                                                                  >  1:427992/1‑251 (MQ=255)                                                                                                                                                                                  tgcaagcagTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAGCGGCACAAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTGCAATGGGCGGCGGTCGTCGTCAGGCATCTGACACCATTGATTACAGCGTCGGCtt                                                                 <  2:280967/242‑1 (MQ=255)                                                                                                                                                                                      agcagTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAGCGGCACAAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTGCAATGGGCGGCGGTCGTCGTCAGGCATCTGACACCATTGATTACAGCGTCGGCTTTACTg                                                            >  1:337401/5‑251 (MQ=255)                                                                                                                                                                                               ggACAACGGTAAAGCGGCAGAAGTCGTTGGTCGTAGGGTAGCGGCACAAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGAGACCCGCGCGCTGGGGATGGCAGTGGTTGCAATGGGCGGCGGTCGTCGTCAGGCATCGGACACCATTGATTACAGCGTCGGCTTTACTGATATGgcg                                                    <  2:237042/250‑1 (MQ=255)                                                                                                                                                                                                      ggTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAGCGGCACAAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTGCAATGGGCGGCGGTCGTCGTCAGGCATCTGACACCATTGATTACAGCGTCGGCTTTACTGATATGGCGCGTTTggg                                            >  1:99819/1‑251 (MQ=255)                                                                                                                                                                                                           aGCGGCAGAAGTCTTTGGTCGTATGGTAGCGGCACAAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTGCAATGGGCGGCGGTCGTCGTCAGGCATCTGACACCATTGATTACAGCGTCGGCTTTACTGATATGGCGCGTTTGGGCGAcc                                       >  1:375919/1‑251 (MQ=255)                                                                                                                                                                                                                    aGTCTTTGGTCGTATGGTAGCGGCACAAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTGCAATGGGCGGCGGTCGTCGTCAGGCATCTGACACCATTGATTACAGCGTCGGCTTTACTGATATGGCGCGTTTGGGCGACCAGGTAGACg                              >  1:41726/1‑251 (MQ=255)                                                                                                                                                                                                                                              aaaaaGGCCCAACCGTCTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTGCAATGGGCGGCGGTCGTCGTCAGGCATCTGACACCATTGATTACAGCGTCGGCTTTACTGATATGGCGCGTTTGGGCGACCAGGTAGACGGTCAGCGTCCGCTGGCGGTTATCCat    <  2:93133/251‑2 (MQ=255)                                                                                                                                                                                                                                                aaaGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTGCAATGGGCGGCGGTCGTCGTCAGGCATCTGACACCATTGATTACAGCGTCGGCTTTACTGATATGGCGCGTTTGGGCGACCAGGTCGACGGTccgc                        >  2:212870/1‑226 (MQ=255)                                                                                                                                                                                                                                                aaaGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTGCAATGGGCGGCGGTCGTCGTCAGGCATCTGACACCATTGATTACAGCGTCGGCTTTACTGATATGGCGCGTTTGGGCGACCAGGTAGACGGTCAGc                        <  1:212870/229‑1 (MQ=255)                                                                                                                                                                                                                                                aaaGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTGCAATGGGCGGCGGTCGTCGTCAGGCATCTGACACCATTGATTACAGCGTCGGCTTTACTGATATGGCGCGTTTGGGCGACCAGGTAGACGGTCAGCGTACGCTGGCGGGTATGCAccg  >  2:42325/1‑249 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                   ACATGAATCAGGTACTGGCCTCCAGTGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGCAGTTCCTGACGGGTGAATATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTGATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTGCAGGCGGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAGCGGCACAAAAAGGCCCGACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTGCAATGGGCGGCGGACGCCGTCAGGCATCTGACACCATCGATTACAGCGTCGGCTTTACTGATATGGCGCGTCTGGGCGACCAGGTAGACGGTCAGCGTCCGCTGGCGGTTATCCACGC  >  NC_000913/4618950‑4619439

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑