Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,619,080	G→T	L284L (CTG→CTT)	deoA →	thymidine phosphorylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,619,080	0	G	T	96.6%	186.5 / ‑1.9	59	L284L (CTG→CTT)	deoA	thymidine phosphorylase
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base T (30/27);  total (31/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.18e-01

GTGAAAGTGGGTAGCGGCGCGTTTATGCCGACCTACGAACTCTCTGAAGCCCTTGCCGAAGCGATTGTTGGCGTGGCTAACGGCGCTGGCGTGCGCACCACCGCGCTGCTCACCGACATGAATCAGGTACTGGCCTCCAGTGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGC‑AGTTCCTGACGGGTGAATATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTGATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTGCAGGCGGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAGCGGCACAAAAAGGCCCGACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTG  >  NC_000913/4618835‑4619321                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                  gTGAAAGGGGGCAGCGGCGCGTTTATGCCGACCTCCGAACTCCCTGAAGCCCGTGCCGAATCGAGTGATGGCGTGGCTATCGGCGCTGGCGTGCGCACCGTCGCGCGGCTCACCGACATGAATCAGTTACTGGCCTCCAGTGCAGGTAACGCGGTGGAAGTTCGTGAAGCGGTGC‑AGTTCCTGACGGGTGAGTATCGTATCCCTCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTAtctc                                                                                                                                                                                                                                              <  2:379735/251‑1 (MQ=255)          ggaaGCGGCGCGTTTATGCCGACCTACGAACTCTCTGAAGCCCTTGCCGAAGCGATTGTTGGCGTGGCTAACGGCGCTGGCGTGCGCACCACCGCGCTGCTCACCGACATGAATCAGGAACTGGCCTCCAGTGCAGGACACGCGGTTGAAGTTCGTGAAGCGGTGCCAGT‑CCTGGCGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTAACGCTGGCGCCGTGCGTGGAGATGCTTATCTCCGGCaaaat                                                                                                                                                                                                                                     >  2:125511/4‑249 (MQ=255)                                ccTACGAACTCTCTGAAGCCCTTGCCGAAGCGATTGTTGGCGTGGCTAACGGCGCTGGCGTGCGCACCACCGCGCTGCTCACCGACATGAATCAGGTACGGGCCTCCAGTGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGC‑CGTTCCTGACGGGAGAGTATCGTAACCCGGGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAcgcg                                                                                                                                                                                                               >  2:172383/1‑251 (MQ=255)                                 cTACGAACTCTCTGAAGCCCTTGCCGAAGCGATTGTTGGCGTGGCTAACGGCGCTGGCGTGCGCACCACCGCGCTGCTCACCGACATGAATCAGGTACTGGCCTCCAGTGCAGGAAACGCGGTTGAAGTTCGTGAAGCGGTGC‑AGTTCCTGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAgcgc                                                                                                                                                                                                              >  1:451083/1‑251 (MQ=255)                                  tACGAACTCTCTGAAGCCCTTGCCGAAGCGATTGTTGGCGTGGCTAACGGCGCTGGCGTGCGCACCACCGCGCTGCTCACCGACATGAATCAGGTACTGGCCTCCAGTGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGC‑AGTTCCTGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAgcgcg                                                                                                                                                                                                             >  1:10154/1‑251 (MQ=255)                                   aCGAACTCTCTGAAGCCCTTGCCGAAGCGATTGTTGGCGTGGCTAACGGCGCTGGCGTGCGCACCACCGCGCTGCTCACCGACATGAATCAGGTACTGGCCTCCAGTGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGC‑AGTTCCTGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAgcgcgc                                                                                                                                                                                                            >  1:237042/1‑251 (MQ=255)                                   aCGAACTCTCTGAAGCCCTTGCCGAAGCGATTGTTGGCGTGGCTAACGGCGCTGGCGTGCGCACCACCGCGCTGCTCACCGACATGAATCAGGTACTGGCCTCCAGTGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGC‑AGTTCCTGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAgcgcg                                                                                                                                                                                                             <  1:277250/250‑1 (MQ=255)                                      aaCTCTCTGAAGCCCTTGCCGAAGCGATTGTTGGCGTGGCTAACGGCGCTGGCGTGCGCACCACCGCGCTGCTCACCGACATGAATCAGGTACTGGCCTCCAGTGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGC‑AGTTCCTGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGcgcgcgcg                                                                                                                                                                                                         <  1:275876/251‑1 (MQ=255)                                                       gCCGAAGCGATTGTTGGCGTGGCTAACGGCGCTGGCGTGCGCACCACCGCGCTGCTCACCGACATGAATCAGGTACTGGCCTCCAGTGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGC‑AGTTCCTGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTGCAAGCAGTGCt                                                                                                                                                                                        >  1:198413/1‑251 (MQ=255)                                                           aaGCGATTGTTGGCGTGGCTAACGGCGCTGGCGTGCGCACCACCGCGCTGCTCACCGACATGAATCAGGTACTGGCCTCCAGTGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGC‑AGTTCCTGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTGCAAGCAGTGCTGGAc                                                                                                                                                                                    >  1:93133/1‑251 (MQ=255)                                                               gATTGTTGGCGTGGCTAACGGCGCTGGCGTGCGCACCACCGCGCTGCTCACCGACATGAATCAGGTACTGGCCTCCAGTGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGC‑AGTTCCTGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTGCAAGCAGTGCTGGACAACg                                                                                                                                                                                <  1:185994/251‑1 (MQ=255)                                                                gttgttgGCGTGGCTAACGGCGCTGGCGTGCGCCCCACCGCGCTGCTCACCGACATGAATCAGGTACTGGCCTCCAGTGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGC‑AGTTCCTGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTGCAAGCAGTGCTGGACAACgg                                                                                                                                                                               <  2:59938/250‑1 (MQ=255)                                                                gttgttgGCGTGGCTAACGGCGCTGGCGGGCGCACCACCGCGCTGCTCACCGACATGAATCAGGTACTGGCCTCCAGGGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGC‑AGTTCCTGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTGCAAGCAGTGCTGGACAACgg                                                                                                                                                                               <  2:150030/250‑1 (MQ=255)                                                                     tgGCGTGGCTAACGGCGCTGGCGTGCGCACCACCGCGCTGCTCACCGACATGAATCAGGTACTGGCCTCCAGTGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGC‑AGTTCCTGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTGCAAGCAGTGCTGGACAACGGTaaa                                                                                                                                                                           <  1:481296/250‑1 (MQ=255)                                                                                   gggttGGCGTGCCCACCGCCGCGCGGCTCATCGCCATGAACTAGGTACTGGCCTCCAGTGCAGGGAACGCGGTTGAAGTTCGTGAAGGGGTGC‑AGTTCCTGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTGCAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCttt                                                                                                                                                            <  2:254799/247‑1 (MQ=255)                                                                                      cTGGCGTGCGCACCACCGCGCTGCTCACCGACATGAATCAGGTACTGGCCTCCAGTGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGC‑AGTTCCTGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTGCAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGt                                                                                                                                                         >  1:129078/1‑251 (MQ=255)                                                                                        ggCGTGCGCACCACCGCGCTGCTCACCGACATGAATCAGGTACTGGCCTCCAGTGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGC‑AGTTCCTGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTGCAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGt                                                                                                                                                         <  1:45486/249‑1 (MQ=255)                                                                                                 accaccGCGCTGCTCACCGACATGAATCGGGTACTAGCCTCCAATGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGC‑AGTTCCTGACGGGTGAGTATCGTCACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGCAATTGCAAGCAGTGCTGGACAACGGTAAAGCGGCAGCAGTCTTTGGTCGTATGGTAGc                                                                                                                                              >  1:428508/1‑251 (MQ=255)                                                                                                     ccGCGCTGCTCACCGACATGAAACAGGTACGGGCCTCCAGTGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGC‑AGTTCCTGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTGCAAGCAGGGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTATCGGca                                                                                                                                          <  2:200148/251‑1 (MQ=255)                                                                                                          ctgctCACCGATATGAATCAGGTACTGGCCTCCAGTGCAGGTAACGCGGTTGAAGTTCATGAAGCGGTGC‑AGTTCCTGACGGGTGAATATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTGATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTGCAGGCGGTGCTGGACAACGGTAAAGCGGCAGAAGGCTTTGGGCGTATGGTAGCGGCACaaa                                                                                                                                      >  1:98242/1‑250 (MQ=255)                                                                                                                cccgacaggagttaGGTACTGGCCACCAGTGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGC‑AGTTCCTGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTGCAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAGCGGCACAAAAAGTccc                                                                                                                               <  2:319490/238‑1 (MQ=255)                                                                                                                    aCATGAATCGGGTACTGGCCTCCATGGCAGGTAACGCGGTGGAAGTTCGTGAAGCGGTGC‑AGTTCCTGACGGGTGAGTATCGTATCCCGCTGCTGTTTGATGTCACGATGGCGCCGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTGCAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAGCGGCACAAAAAGGCccaacc                                                                                                                           <  2:469309/251‑1 (MQ=255)                                                                                                                    aCATGAATCAGGTACTGGCCTCCAGTGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGC‑AGTTCCTGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTGCAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAGCGGCACAAAAAGGCccaacc                                                                                                                           <  1:355513/251‑1 (MQ=255)                                                                                                                      aTGAATCAGGTACTGGCCTCCAGTGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGC‑AGTTCCTGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGACGCGCGCGCGAAATTGCAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGGCTTTGGTCGTATGGTAGCGGCACACAAAGGCccaaccg                                                                                                                          >  2:407745/1‑250 (MQ=255)                                                                                                                          aTCAGGTACTGGCCTCCAGTGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGC‑AGGTCCTGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTGCAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAGCGGCACAAAAAGGCCCAACCGACTTc                                                                                                                     <  2:10154/251‑1 (MQ=255)                                                                                                                            cAGGTTCTGGCCGCCAGTGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGC‑AGTTCCTGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCACCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTGCAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCGGTGGTCGTATGGTAGCGGCACAAAAAGGACCCACCGACTTCg                                                                                                                    <  2:59595/250‑1 (MQ=255)                                                                                                                                       cTCCAGTGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGC‑AGTTCCTGGCGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGAGGAGATGCTTCTCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTGCAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAGCGGCACAAAACGGCCCAACCGCCTTCGTTGAGAACTAcg                                                                                                        >  2:81056/1‑251 (MQ=255)                                                                                                                                         ccAGTGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGC‑AGTTCCTGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTGCAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAGCGGCACAAAAAGGCCCAACCGACTTCGTTGAGAACTAcgcg                                                                                                      >  1:1083/1‑251 (MQ=255)                                                                                                                                             tGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGC‑AGTTCCTGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTGCAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAGCGGCACAAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGt                                                                                                  >  1:6987/1‑251 (MQ=255)                                                                                                                                             ggtagggaacgcggtgtaattgcgggAAGCGGTGC‑TTTTCCCGACGGGGGAGTATCGTAACCCGCGTCTTTTTGATGTCACGATGGCGCTGGGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTGCAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAGCGGCACAAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGt                                                                                                  <  2:399139/226‑1 (MQ=255)                                                                                                                                               cAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGC‑AGTTCCTGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTGCAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAGCGGCACAAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTAt                                                                                                >  1:478238/1‑251 (MQ=255)                                                                                                                                                aGGTAACGCGGTTGAAGTTCGTGAAGCGGTGC‑AGTTCCTGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTGCAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAGCGGCACAAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATc                                                                                               >  1:435103/1‑251 (MQ=255)                                                                                                                                                 ggTAACGCGGTTGAAGTTCGTGAAGCGGTGC‑AGTTCCTGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTGCAAGCAGTGCTGGACAACGGAAAAGCGGCAGAAGTCTTTGGTCGTATGGTAGCGGCACAAACAGGCCCCACCGACTTCGTTGCGAACTACGCGAGGTATCt                                                                                              >  2:267944/1‑251 (MQ=255)                                                                                                                                                  gTAACGCGGTTGAAGTTCGTGAAGCGGTGC‑AGTTCCTGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTGCAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAGCGGCACAAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCTg                                                                                             <  2:24726/251‑1 (MQ=255)                                                                                                                                                    aaCGCGGTTGAAGTTCGTGAAGCGGTGC‑AGTTCCTGACAGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTGCAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAGCGGCACAAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCTGcc                                                                                           >  1:90088/1‑251 (MQ=255)                                                                                                                                                        gggTTGAAGTTCTGGAAGCGGTGC‑AGTTCCTGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTGCAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAGCGGCACAAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACa                                                                                       <  2:359745/250‑1 (MQ=255)                                                                                                                                                                gTTCGTGAAGCGGTGC‑AGTTCCTGACGTGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTGCAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAGCGGCACAAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCt                                                                               <  2:331050/251‑1 (MQ=255)                                                                                                                                                                gTTCGTGAAGCGGTGC‑AGTTCCTGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTGCAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAGCGGCACAAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCt                                                                               <  2:155417/251‑1 (MQ=255)                                                                                                                                                                gTTCGTGAAGCGGTGC‑AGTTCCTGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTGCAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAGCGGCACAAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCt                                                                               >  1:331050/1‑251 (MQ=255)                                                                                                                                                                   cGTGAAGCGGTGC‑AGTTCCTGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTGCAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAGCGGCACAAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGAc                                                                            >  1:108423/1‑251 (MQ=255)                                                                                                                                                                    atGAAGCGGTGC‑AGTTCCTGACGGGTGAATATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTGATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTGCAGGCGGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAGCGGCACAAAAAGGCCCGACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACg                                                                           <  2:98242/250‑1 (MQ=255)                                                                                                                                                                       aaGCGGTGC‑AGTTCCTGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTGCAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAGCGGCCCAAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGCAGTATCTGCCGACAGCGATGCTGACGaa                                                                         >  2:248331/1‑250 (MQ=255)                                                                                                                                                                                   ttCCTGACGGGTGAGTATCGTAACCCGCGTCTGTTTGCTGTCACGATGGCGCTGGGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTGCAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAGCGGCACAAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGCCGAAAGAAGTCTATGc                                                             >  2:483270/1‑251 (MQ=255)                                                                                                                                                                                       tGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTGCAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAGCGGCACAAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGAt                                                         >  1:280967/1‑251 (MQ=255)                                                                                                                                                                                        gACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTGCAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAGCGGCACAAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGa                                                          <  1:234984/249‑1 (MQ=255)                                                                                                                                                                                        gACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTGCAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAGCGGCACAAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATa                                                        <  2:196691/251‑1 (MQ=255)                                                                                                                                                                                                agtatCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTGCACGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAGCGGCACAAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACAAAAGCAGTCTATGCTGATACCGAAGGt                                                >  2:325780/3‑251 (MQ=255)                                                                                                                                                                                                           ccGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTGCAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAGCGGCACAAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTg                                      >  1:244998/1‑250 (MQ=255)                                                                                                                                                                                                                gcTGTTTGATGTCACGATGGCTCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGAGGACGCCGAAGCGCGCGCGAAATTGCAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAGCGGCACAAAAAGTCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATgg                                <  2:424693/250‑1 (MQ=255)                                                                                                                                                                                                                  tGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAACGATGACGCCGAAGCGCGCGCGAAATTGCAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAGCGGCACAAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGCTACCGAAGGTTTTGTCAGTGCAATGGAt                              >  1:64961/1‑251 (MQ=255)                                                                                                                                                                                                                   gttggaggTCACGAGGGGGCTGTGCGTGGAGAGGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGTAATTGCAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAGCGGCACAAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATa                             <  2:112292/244‑1 (MQ=255)                                                                                                                                                                                                                   gTTTGATTGCACGATGGCGCGGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTGCAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAGCGGCACAAAAAGGCACAACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGAt                              <  2:122097/250‑1 (MQ=255)                                                                                                                                                                                                                   gTTTGATGTCACGATGGCGCTGTTCGTGGAGATGCTTATCTCCGTCAAACTGGTGAAAGATGACGCCGAAGCGCGCGCGAAATTGCAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAGCGGCACAAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGAt                              <  2:1083/250‑1 (MQ=255)                                                                                                                                                                                                                     ttGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTGCAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAGCGGCACAAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATAcc                           >  1:4898/1‑251 (MQ=255)                                                                                                                                                                                                                               gATGGCGCTGTGCGTGGAGATGCTTATCTCCGGAAAACTGGCGCAAGATGACGCCGAAGCGCGCGCGAAATTGCAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAGCGGCACAAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATACTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTgg                 >  1:131448/1‑251 (MQ=255)                                                                                                                                                                                                                                    cgcTGTGCGCTGAGCTGCTTATCTCAGGCAAACCGGCGAAAGATGGCGCCGACGCGCGAGCGACATTGCAAGCAGTGCTGGACACCGGTAAAGCGGCAGAAGTCTTTGGTCGTATCGTACCGGCACAAAAAGGCCCAACCGCCTTCGTTGAGAATTGAGCGAAGTATCTGCCGTCCGCGCTGCTGACGAAAGAAGACAATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCAGCGCGCTggggttg            >  2:236244/1‑248 (MQ=255)                                                                                                                                                                                                                                      cTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTGCAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAGCGGCACAAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGGGATGGc          >  1:162842/1‑251 (MQ=255)                                                                                                                                                                                                                                              ggAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTGCAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAGCGGCACAAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTg  <  1:105605/251‑1 (MQ=255)                                                                                                                                                                                                                                              ggAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTGCAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAGCGGCACAAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGAGGTTg  >  2:408159/1‑251 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                  GTGAAAGTGGGTAGCGGCGCGTTTATGCCGACCTACGAACTCTCTGAAGCCCTTGCCGAAGCGATTGTTGGCGTGGCTAACGGCGCTGGCGTGCGCACCACCGCGCTGCTCACCGACATGAATCAGGTACTGGCCTCCAGTGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGC‑AGTTCCTGACGGGTGAATATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTGATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTGCAGGCGGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAGCGGCACAAAAAGGCCCGACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTG  >  NC_000913/4618835‑4619321

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑