Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,618,483	C→T	H85H (CAC→CAT)	deoA →	thymidine phosphorylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,618,483	0	C	T	100.0%	161.1 / NA	52	H85H (CAC→CAT)	deoA	thymidine phosphorylase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (26/26);  total (26/26)

GAAATTATTCGTAAAAAACGTGATGGTCATGCGCTGAGCGATGAAGAAATTCGTTTCTTTATCAACGGTATTCGCGACAACACTATCTCCGAAGGGCAGATTGCCGCCCTCGCGATGACCATTTTCTTCCACGATATGACAATGCCTGAGCGTGTCTCGCTGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACACTCCACCGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTCCGATGATCTCTGGTCGCGGCCTCGGTCATACTGGCGG‑TACGCTCGACAAACTGGAATCC‑ATCCCTGGCTTCGAC‑ATTTTCCCGGATGACAACCGTTTCCGCGAAATTATTAAAGACGTCGGCGTGGCG‑ATTATCGGTCAGACCAGTTCACTGGCTCCGGCTGATAA  >  NC_000913/4618244‑4618722                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                    gAAATTATTCGTAAAAAACGTGATGGTCATGCACTGAGCGATGAAGAAATTCGTTTCTTTATCAACGGCATTCGCGACAACACTATCTCCGAAGGGCAGATTGCCGCCCTCGCGATGACCATTTTCTTCCACGATATGACAATGCCTGAGCGTGTCTCGCTGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACtggtgg                                                                                                                                                                                                                                          <  1:311109/251‑1 (MQ=255)         tCGTAAAAAACGTGATGGTCATGCACTGAGCGATGAAGAAATTCGTTTCTTTATCAACGGCATTCGCGACAACACTATCTCCGAAGGGCAGATTGCCGCCCTCGCGATGACCATTTTCTTCCACGATATGACAATGCCTGAGCGTGTCTCGCTGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCg                                                                                                                                                                                                                                  >  1:409343/1‑251 (MQ=255)             aaaaaaCGTGATGGTCATGCACTGAGCGATGAAGAAATTCGTTTCTTTATCAACGGCATTCGCGACAACACTATCTCCGAAGGGCAGATTGCCGCCCTCGCGATGACCATTTTCTTCCACGATATGACAATGCCTGAGCGTGTCTCGCTGACCATGGCGATGCGAGACTCAGGAACCGTTCTCGCCTGGAAAAGCCTGCATCTGAATGGCCCGCTTGTTGATAAACATTCCACTGGTGGCGTGGGCGAtgg                                                                                                                                                                                                                              >  1:379735/1‑250 (MQ=255)               aaaaCGTGATGGTCATGCACTGAGCGATGAAGAAATTCGTTTCTTTATCAACGGCATTCGCGACAACACTATCTCCGAAGGGCAGATTGCCGCCCTCGCGATGACCATTTTCTTCCACGATATGACAATGCCTGAGCGTGTCTCGCTGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGAtgtg                                                                                                                                                                                                                             >  1:290734/1‑250 (MQ=255)                            cATGCACTGAGCGATGAAGAAATTCGTTTCTTTATCAACGGCATTCGCGACAACACTATCTCCGAAGGGCAGATTGCCGCCCTCGCGATGACCATTTTCTTCCACGATATGACAATGCCTGAGCGTGTCTCGCTGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGtt                                                                                                                                                                                                               >  1:193234/1‑251 (MQ=255)                               gctcggAGCGATGAAGAAATTCGTTTCTTTATCAACGGCACTCGCGACAACACTATCTCCGAAGGGCAGATTGCCGCCCTCGCGATGACCATTTTCTTCCACGATATGACAATGCCTGAGCGTGTCTCGCTGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGGTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTgg                                                                                                                                                                                                             <  2:346753/245‑1 (MQ=255)                                   tGAGCGCTGAAGAAATTAGTTACTTTATCTACGGCATTAGCGACAACACTATCTCAGAAGGGCAGATTGCCGCCCTCGCGATGCCCATTTTCTTACACGATATGACAATGCCTGAGCGTGTCTCGCTGACCATGGCTATGCGAGCTTCAGGAACCGTTCTCGACTGGAAAAGCCTGAATCTGAATGGCCCGATTGTTGATAAACATTACCCTGGAGGCGTGGGCGATATGACTTCGCAGTTGTTggagc                                                                                                                                                                                                          >  2:284241/1‑246 (MQ=255)                                             agaaATTCGTTTCTTTATCAACGGCATTCGCGACAACACTATCTCCGAAGGGCAGATTGCCGCCCTCGCGATGACCATTTTCTTCCACGATATGACAATGCCTGAGCGTGTCTCGCTGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAg                                                                                                                                                                                              <  1:416897/251‑1 (MQ=255)                                               aaaTTCGTTTCTTTAACAACGGCATTCGCGACAACACTATCTCCGAAGGGCAGATTGCCGCCCTCGCGATGACCATTTTCTTCCACGATATGACAATGCCTGAGCGTGTCTCGCTGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGcc                                                                                                                                                                                            <  1:433874/251‑1 (MQ=255)                                                       ttCTTTATCAACGGCATTCGCGACAACACTATCTCCGAAGGGCAGATTGCCGCCCTCGCGATGACCATTTTCTTCCACGATATGACAATGCCTGAGCGTGTCTCGCTGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGCGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGcggcgg                                                                                                                                                                                    >  2:424468/1‑251 (MQ=255)                                                        tCTTTATCAACGGCATTCGCGACAACACTATCTCCGACGGGCAGATTGCCGCCCTCGCGATGACCATTTTCTTCCACGATATGACAATGCCTGAGCGTGTCTCGCTGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGCTAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCggcggc                                                                                                                                                                                   >  2:66913/1‑251 (MQ=255)                                                          tttATCAACGGCATTCGCGACAACACTATCTCCGAAGGGCAGATTGCCGCCCTCGCGATGACCATTTTCTTCCACGATATGACAATGCCTGAGCGTGTCTCGCTGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGcggcgggc                                                                                                                                                                                  >  2:365809/1‑248 (MQ=255)                                                                aaCGGCATTCGCGACAACACTATCTCCGAAGGGCAGATTGCCGCCCTCGCGATGACCATTTTCTTCCACGATATGACAATGCCTGAGCGTGTCTCGCTGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGCCTTCGATGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTcc                                                                                                                                                                           >  1:468739/1‑251 (MQ=255)                                                                aaCGGCATTCGCGAAAACACTATCTCCGAAGGGCAGATTGCCGCCCTCGCGATGACCATTTTCTTCCACGATATGACAATGCCTGAGCGTGTCTCGCTGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTcc                                                                                                                                                                           <  1:246726/251‑1 (MQ=255)                                                                            gACAACACTATCTCCGAAGGGCAGATTGCCGCCCTCGCGATGACCATTTTCTTCCACGATATGACAATGCCTGAGCGTGTCTCGCTGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTCCGATGTTCTCTgg                                                                                                                                                               >  1:389216/1‑251 (MQ=255)                                                                             acaacaCTATCTCCGAAGGGCAGATTGCCGCCCTCGCGATGACCATTTTCTTCCACGATATGACAATGCCTGAGCGTGTCTCGCTGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGGTGGTCGCAGCCTGCGGCGGCCATATTCCgcttgtttctggg                                                                                                                                                              >  2:51560/1‑239 (MQ=255)                                                                                 cacTATCTCCGAAGGGCAGATTGCCGCCCTCGCGATGACCATTTTCTTCCACGATATGACAATGCCTGAGCGTGTCTCGCTGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTCCGATGATCTCTGGTcgcg                                                                                                                                                          >  1:390536/1‑251 (MQ=255)                                                                                         ccGAAGGGCAGATTGCCGCCCTCGCGATGACCATTTTCTTCCACGATATGACAATGCCTGAGCGTGTCTCGCTGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTCCGATGATCTCTGGTCGCGGCCTc                                                                                                                                                     <  1:418127/248‑1 (MQ=255)                                                                                          cGAAGGGCAGATTGCCGCCCTCGCGATGACCATTTTCTTCCACGATATGACAATGCCTGAGCGTGTCTCGCTGACCATGGCGATGCGAGATTCAGGACCCGTTCTCGCCTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGACGCAGGCTGCGGCGGCTATATTCCGATGGTCACTGGTCGCGGCCCCGGTc                                                                                                                                                 >  2:279764/1‑251 (MQ=255)                                                                                          cGAAGGGCAGATTGCCGCCCTCGCGATGACCATTTTCTTCCACGATATGACAATGCCTGAGCGTGTCTCGCTGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTCAGATGATCGCTGGGCGCGGCCTCGGt                                                                                                                                                  >  2:406816/1‑250 (MQ=255)                                                                                                gCAGATTGCCGCCCTCGCGATGACCATTTTCTTCCACGATATGACAATGCCTGAGCGTGTCTCGCTGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTCCGATGATCTCTGGTCGCGGCCTCGGTCATACTg                                                                                                                                           <  2:193234/251‑1 (MQ=255)                                                                                                                 cgATGACCATTTTCTTCCCCGATATGACAATGCCTGAGCGGGTCTCGCTGACCAGGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTCCGATGATCTCTGGTCGCGGCCTCGGTCATACTGGCGG‑TACGCTCGACaaa                                                                                                                         <  2:390536/251‑1 (MQ=255)                                                                                                                  gATGACCATTTTCTTCCACGATATGACAATGCCTGAGCGTGTCTCGCTGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTCCGATGCTATCTGGACGCGACCTCCGTCATACTGGCGG‑TACGCTCGACGAAc                                                                                                                        >  1:59595/1‑251 (MQ=255)                                                                                                                  gATGACCATTTTCTTCCACGATATGACAATGCCTGAGCGTGTCTCGCTGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCAACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGACGCCTGCGGAGGCCATATTCCGATGAGCTTTGGTCGCGGCCTCAGTTCTTCTGGCGG‑CTCGCTCGAccca                                                                                                                         >  2:331936/1‑247 (MQ=255)                                                                                                                     gACCATTTTCTTCCACGATATGACAATGCCTGAGCGTGTCTCGCTGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGCTGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTCCGATGATCTCTGGTCGCGTCCTCGGCCATCCAGGCGG‑TACGccggacacaccgg                                                                                                                     >  2:368634/1‑239 (MQ=255)                                                                                                                          ttttCTTCCACGATATGACAATGCCTGAGCGTGTCTCGCTGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTCCGATGATCTCTGGTCGCGGCCTCGGTCATACTGGCGG‑TACGCTCGACAAACTGGAatc                                                                                                                 <  1:292293/250‑1 (MQ=255)                                                                                                                                tcCACGATATGACAATGCCTGAGCGTGTCTCGCTGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTCCGATGATCTCTGGTCGCGGCCTCGGTCATACTGGCGG‑TACGCTCGACAAACTGGAatcc‑atcc                                                                                                           <  1:147167/249‑1 (MQ=255)                                                                                                                                tcCACGATATGACAATGCCTGAGCGTGTCTCGCTGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTCCGATGATCTCTGGTCGCGGCCTCGGTCATACTGGCGG‑TACGCTCGACAAACTGGAatcc‑atc                                                                                                            <  1:376472/248‑1 (MQ=255)                                                                                                                                tcCACGATATGACAATGCCTGAGCGTGTCTCGCTGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTCCGATGATCTCTGGTCGCGGCCTCGGTCATACTGGCGG‑TACGCTCGACAAACTGGAATCC‑ATccc                                                                                                          <  1:66913/250‑1 (MQ=255)                                                                                                                                 ccACGATATGGCAATGCCTGAGCGTGTCTCGCTGACCATGTCGAGGCGTGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAAGGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTCCGATGATCTCTGGTCGCGGCCTCGGTCATACTGGCGG‑TACGCTCGACAAACTGGAATCC‑ATCCCt                                                                                                         <  2:381645/250‑1 (MQ=255)                                                                                                                                  cACGATATGACAATGCCTGAGCGTGTCTCGCTGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTCCGATGATCTCTGGTCGCGGCCTCGGTCATACTGGCGG‑TACGCTCGACAAAACGGAATTC‑ATCCCTgg                                                                                                       >  1:207518/1‑251 (MQ=255)                                                                                                                                      agatgccAATGCCTGGGCGTGTCTCGCAGACCATGGCGATGCGTGATGCAGGAACCGTTCTCGACCGGAAAAGCCTGCATCGTAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCTTCGGCGATGTGGCTTCGCTGATGTTGGGTCCGATGGTCGCAGCCTGCGGCGGCTATATTCCGATGATCTCTGGTCGCGGCCTCGGGCATACTGGCGG‑TACGCTCGACAAACTGGAATCC‑ATCCCTGGCTTc                                                                                                   <  2:190817/245‑1 (MQ=255)                                                                                                                                      aaatGACAATGCCGGAGCGTGGCTCGCTTACCATGGCGATGCGAGAGTCAGGAACCGTTCTCGACGTGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCTCTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTCCGAGGATCTCTGGTCGCTGCCGCGGTCATACTGGCGG‑TACGCTCGACAAACTGGAATCC‑ATCCCTGGCTTc                                                                                                   <  2:480424/249‑1 (MQ=255)                                                                                                                                        atGACAATGCCTGAGCGTGTCTCGCTGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGCCTGGACAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTCCGTTGATCTCTTGGCGGCGCCTCGGTCCTACTGGCGGATCCG‑TCGCCCAACTGGAAtcc‑ctccccgggtttac                                                                                                 >  2:103966/1‑242 (MQ=255)                                                                                                                                            cAATGCCTGAGCGTGTCTCGCTGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCtata                                                                                                                                                                               >  2:75763/1‑171 (MQ=255)                                                                                                                                            cAATGCCTGAGCGTGTCTCGCTGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCtata                                                                                                                                                                               <  1:75763/171‑1 (MQ=255)                                                                                                                                                   tGAGCGTGTCTCGCTGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGCAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTCCGGTGATCTCTGGTCGCGGCCTCGGTCATACTGGCGG‑GACGCTCGACaaaa                                                                                                                        >  1:410522/1‑217 (MQ=255)                                                                                                                                                   tGAGCGTGTCTCGCTGACCATGGCGATGCGAGATCCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTCCGATGATCTCTGGTCGCGGCCTCGGTCATACTGGCGG‑TACGCTCGACAAAc                                                                                                                        <  2:410522/218‑1 (MQ=255)                                                                                                                                                             ccGCTGACCATGGCGATGTGAGAGTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTCCGATGATCTCTGGTCGCGGCCTCGGTCATACTGGCGG‑TACGCTCGACAAACTGGAATCC‑ATCCCTGGCTTCGAC‑ATTTTCCCGGATGACAACCg                                                                           <  1:260854/250‑1 (MQ=255)                                                                                                                                                              cGCTGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTCCGATGATCTCTGGTCGCGGCCTCGGTCATACTGGCGG‑TACGCTCGACAAACTGGAATCC‑ATCCCTGGCTTCGAC‑ATTTTCCCGGATGACAAcc                                                                            <  2:379233/249‑1 (MQ=255)                                                                                                                                                              cGCTGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTCCGATGATCTCTGGTCGCGGCCTCGGGCATACTGGCGG‑TACGCTCGGCAAACTGGGATCC‑ATCCCTGGCTTCGGC‑AATTTCCCGGATGACaaacgg                                                                          >  1:200148/1‑247 (MQ=255)                                                                                                                                                                     cATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTCCGATGATCTCTg                                                                                                                                                                <  2:22990/161‑1 (MQ=255)                                                                                                                                                                     cATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTCCGATGATCTCTg                                                                                                                                                                >  1:22990/1‑161 (MQ=255)                                                                                                                                                                     cATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTCCGATGATCTCTGGTCGCGGCCTCGGTCATACTGGCGG‑TACGCTCGACAAACTGGAATCC‑ATCCCTGGCTTCGAc                                                                                                <  2:134497/223‑1 (MQ=255)                                                                                                                                                                                                tctcGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTCCGATGGTCTCTGGTCGCGGCCTCGGTCATACTGGCGG‑GACGCTCGGCAAACTGGAATCC‑ATCCCCGGCTTCGCCCTTTTTCCCGG‑GGGCAAACGTTTCCCCGAAAATTTTAAAAACATCGGCggggggg                                        >  2:454073/1‑245 (MQ=255)                                                                                                                                                                                                   cGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTCCGATGATCTCTGGGCGCGGCCTCGGTCATACTGGCGG‑TAATCCCGACAAAATGGAATCCCTTCCCGGGCT‑CGAC‑ATTTTCCCCGGTGACAACCGTTTCCCCGGAATTTTTAAAGACGTCGGCGTgggggttt                                     >  2:169502/1‑245 (MQ=255)                                                                                                                                                                                                        gaaaaatcttgcttctaaaTGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTAACTGCTATAATTTTGGGGCCTATGGTCGCAGCCTGCGGCGGCTATATTTCGATGATCTCTGGTCGCGGCCTCGGTAATCCTGGCGG‑TACGCTCGACAAACTGGAATCC‑ATCCCTGGCTTCAAC‑ATTTTCCCGGATGACAACCGTTTCCGCGAAATTATTAAAGACGTCGGCGTGGCG‑ATTATCgg                                <  1:3408/233‑1 (MQ=255)                                                                                                                                                                                                                  gCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTCCGATGATCTCTGGTCGCGGCCTCGGTCATACTGGCGG‑TACGCTCGACAAACTGGAATCC‑ATCCCTGGCTTCGAC‑ATTTTCCCGGATGACAACCGTTTCCGCGAAATTATTAAAGACGTCGGCGTGGCG‑ATTATCGGTCAGACCAgc                      <  1:243161/250‑2 (MQ=255)                                                                                                                                                                                                                      cTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTCCGATGATCTCTGGTCGCGGCCTCGGTCATACTGGCGG‑TACGCTCGACAAACTGGAATCC‑ATCCCTGGCTTCGAC‑ATTTTCCCGGATGACAACCGTTTCCGCGAAATTATTAAAGACGt                                                   <  1:209821/218‑1 (MQ=255)                                                                                                                                                                                                                              cccGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTCCGATGATCTCTGGTCGCGGCCTCGGTCATACTGGCGG‑TACGCTCGACAAACTGGAATCC‑ATCCCTGGCTTCGAC‑ATTTTCCCGGATGACAACCGTTTCCGCGAAATTATTAAAGACGTCGGCGTGGCG‑ATTATCGGTCAGACCAGCTCACTGGCTCCgg         <  2:344859/251‑1 (MQ=255)                                                                                                                                                                                                                                 gATTGTTGATAAACATTCCACGGGGGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTCCGATGATCTCTGGTCGCGGCCTCGGTCATACTGGCGG‑TACGCTCGACAAACTGGAATCC‑ATCCCTGGCTTCGAC‑ATTTTCCCGGAGGACAACCGTTTCCGCGAAATTATTAAAGACGTCGGCGTGGCG‑ATTATCGGTCAGACCAGCTCACTGGCTCCGGcgg      <  2:409343/251‑3 (MQ=255)                                                                                                                                                                                                                                      ttgATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTCCGATGATCTCTGGTCGCGGCCTCGGTCATACTGGCGG‑TACGCTCGACAAACTGGAATCC‑ATCCCTGGCTTCGGC‑ATTTTCCCGGATGCCAACCGTTTCCGCGAAATTATTAAAAACGTCGGCGGGGCGGATT‑TCGGTCAGACCaccccaccggctcccgcgggtaa  >  1:274578/1‑228 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                    GAAATTATTCGTAAAAAACGTGATGGTCATGCGCTGAGCGATGAAGAAATTCGTTTCTTTATCAACGGTATTCGCGACAACACTATCTCCGAAGGGCAGATTGCCGCCCTCGCGATGACCATTTTCTTCCACGATATGACAATGCCTGAGCGTGTCTCGCTGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACACTCCACCGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTCCGATGATCTCTGGTCGCGGCCTCGGTCATACTGGCGG‑TACGCTCGACAAACTGGAATCC‑ATCCCTGGCTTCGAC‑ATTTTCCCGGATGACAACCGTTTCCGCGAAATTATTAAAGACGTCGGCGTGGCG‑ATTATCGGTCAGACCAGTTCACTGGCTCCGGCTGATAA  >  NC_000913/4618244‑4618722

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑