Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,618,312	T→C	G28G (GGT→GGC)	deoA →	thymidine phosphorylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,618,312	0	T	C	98.2%	167.2 / ‑4.6	55	G28G (GGT→GGC)	deoA	thymidine phosphorylase
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base C (26/28);  total (26/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.43e-01

GTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATCAGGCCTACGAGACGTATCTACCCGTAGGCCGGATAAGGCGTAGACGCATCCGGCAAAAGCCGCCTCATACTCTTTTCCTCGGGAGGTTACCTTGTTTCTCGCACAAGAAATTATTCGTAAAAAACGTGA‑TGGTCATGCGCTGAGCGATGAAGAAATTCGTTTCTTTATCAACGGTATTCGCGACAACACTATCTCCGAAGGGCAGATTGCCGCCCTCGCGATGACCATTTTCTTCCACGATATGACAATGCCTGAGCGTGTCTCGCTGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACACTCCACCGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTCC  >  NC_000913/4618080‑4618557                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                      gtaggagCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATCAGGCCTACGAGACGTATCTACCCGTAGGCCGGATCAGGCGTAGACGCATCCGGCAAAAGCCGCCTCATACTCTTTTCCTCGGGAGGTTACCTTGTTTCTCGCACAAGAAATTATTCGTAACAAACGTGA‑TGGTCATGCACTGAGCGATGAAGAAATTCGTTACTTTATCATCGGCATTCGCGCCAACACTAtc                                                                                                                                                                                                                                     >  2:383922/5‑251 (MQ=255) ggaaGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATCAGGCCTACGAGACGTATCTACCCGTAGGCCGGATAAGGCGTAGACGCATCCGGCAAAAGCCGCCTCATACTCTGTTCCTCGGGAGGTTACCTTGTTTCTCGCACAAGAAATTATTCGTAAAAAACGTGA‑TGGTCATGCACGGAGCGATGAAGAAATTCGTTTCTTTATCAACGGCATTCGCGACAACACTAtc                                                                                                                                                                                                                                     <  2:211698/249‑1 (MQ=255)   aaGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATCAGGCCTACGAGACGTATCTACCCGTAGGCCGGATAAGGCGTAGACGCATCCGGCAAAAGCCGCCTCATACTCTTTTCCTCGGGAGGTTACCTTGTTTCTCGCACAAGAAATTATTCGTAAAAAACGTGA‑TGGTCATGCACTGAGCGATGAAGAAATTCGTTTCTTTATCAACGGCATTCGCGACAACACTAtctc                                                                                                                                                                                                                                   >  1:164602/1‑251 (MQ=255)   aaGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATCAGGCCTACGAGACGTATCTACCCGTAGGCCGGATAAGGCGTAGACGCATCCGGCAAAAGCCGCCTCATACTCTTTTCCTCGGGAGGTTACCTTGTTTCTCGCACAAGAAATTATTCGTAAAAAACGTGA‑TGGTCATGCACTGAGCGATGAAGAAATTCGTTTCTTTATCAACGGCATTCGCGACAACACTAtctc                                                                                                                                                                                                                                   <  1:39413/251‑1 (MQ=255)   aaGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATCAGGCCTACGAGACGTATCTACCCGTAGGCCGGATAAGGCGTAGACGCATCCGGCAAAAGCCGCCTCATACTCTTTTCCTCGGGAGGTTACCTTGTTTCTCGCACAAGAAATTATTCGTAAAAAACGTGA‑TGGTCATGCACTGAGCGATGAAGAAATTCGTTTCTTTATCAACGGCATTCGCGACAACACTAtct                                                                                                                                                                                                                                    <  1:123714/250‑1 (MQ=255)      agCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATCAGGCCTACGAGACGTATCTACCCGTAGGCCGGATAAGGCGTAGACGCATCCGGCAAAAGCCGCCTCATACTCTTTTCCTCGGGAGGTTACCTTGTTTCTCGCACAAGAAATTATTCGTAAAAAACGTGA‑TGGTCATGCACTGAGCGATGAAGAAATTCGTTTCTTTATCAACGGCATTCGCGACAACACTATCTCCg                                                                                                                                                                                                                                 >  1:322482/1‑250 (MQ=255)             gcagcTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATCAGGCCTACGAGACGTATCTACCCGTAGGCCGGATAAGGCGTAGACGCATCCGGCAAAAGCCGCCTCATACTCTTTTCCTCGGGAGGTTACCTTGTTTCTCGCACAAGAAATTATTCGTAAAAAACGTGA‑TGGTCATGCACTGAGCGATGAAGAAATTCGTTTCTTTATCAACGGCAtt                                                                                                                                                                                                                                                    >  2:88306/1‑224 (MQ=255)             gcagcTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATCAGGCCTACGAGACGTATCTACCCGTAGGCCGGATAAGGCGTAGACGCATCCGGCAAAAGCCGCCTCATACTCTTTTCCTCGGGAGGTTACCTTGTTTCTCGCACAAGAAATTATTCGTAAAAAACGTGA‑TGGTCATGCACTGAGCGATGAAGAAATTCGTTTCTTTATCAACGGCAtt                                                                                                                                                                                                                                                    <  1:88306/224‑1 (MQ=255)                gcTACCAAGTAAGATGTGTTACGCCTGATGTGCTGCGCTTATCAGGCCTACGAGACGTATCTACCCGTAGGCCGGATAAGGCGTAGACGCATCCGGCAAAAGCCGCCTCATACTCTTTTCCTCGGGAGGTTACCTTGTTTCTCGCACAAGAAATTATTCGTAAAAAACGTGA‑TGGTCATGCACTGAGCGATGAAGAAATTCGTTTCTTTATCAACGGCATTCGCGACAACACTATCTCCGAAGGGCAGAtt                                                                                                                                                                                                                      <  2:161187/251‑1 (MQ=255)                          aagATGCTTTACGCCTGATGCGCTGCGCTTATCAGGCCTACGAGACGTATCTACCCGTAGGCCGGATAAGGCGTAGACGCATCCGGCAAAAGCCGCCTCATACTCTTTTCCTCGGGAGGTTACCTTGTTTCTCGCACAAGAAATTATTCGTAAAAAACGTGA‑TGGTCATGCACTGAGCGATGAAGAAATTCGTTTCTTTATCAACGGCATTCGCGACAACACTATCTCCGAAGGGCAGATTGCCGCCCTcg                                                                                                                                                                                                            <  2:164602/251‑1 (MQ=255)                            gATGCTTTACGCCTGATGCGCTGCGCTTATCAGGCCTACGAGACGTATCTACCCGTAGGCCGGATAAGGCGTAGACGCATCCGGCAAAAGCCGCCTCATACTCTTTTCCTCGGGAGGTTACCTTGTTTCTCGCACAAGAAATTATTCGTAAAAAACGTGA‑TGGTCATGCACTGAGCGATGAAGAAATTCGTTTCTTTATCAACGGCATTCGCGACAACACTATCTCCGAAGGGCAGATTGCCGCCCTcgcg                                                                                                                                                                                                          >  1:304476/1‑251 (MQ=255)                                   tACGCCTGATGCGCTGCGCTTATCAGGCCTACGAGACGTATCTACCCGTAGGCCGGATAAGGCGTAGACGCATCCGGCAAAAGCCGCCTCATACTCTTTTCCTCGGGAGGTTACCTTGTTTCTCGCACAAGAAATTATTCGTAAAAAACGTGA‑TGGTCATGCACTGAGCGATGAAGAAATTCGTTTCTTTATCAACGGCATTCGCGACAACACTATCTCCGAAGGGCAGATTGCCGCCCTCGCGATGAcc                                                                                                                                                                                                    <  2:202576/250‑1 (MQ=255)                                    aCGCCTGATGCGCTGCGCTTATCAGGCCTACGAGACGTATCTACCCGTAGGCCGGATAAGGCGTAGACGCATCCGGCAAAAGCCGCCGCATACTCTTTTCCTCGGGAGGTTACCTTGTTTCTCGCACAAGAAATTATTCGTAAAAAACGTGA‑TGGTCATGCACTGAGCGATGAAGAAATTCGTTTCTTTATCAACGGCATTCGCGACAACACTATCTCCGAAGGGCAGATTGCCGCCCTCGCGATGACCAt                                                                                                                                                                                                  <  1:294069/251‑1 (MQ=255)                                    aCGCCTGATGCGCGGCGCTTATCAGGCCTACGAGACGTAGCTACCCGTAGGCCGGATAAGGCGTAGACGCATCCGGCAAAAGCCGCCTCATACTCTTTTCCTCGGGAGGTTACCTTGTTTCTCGCACAAGAAATTATTCGTAAAAAACGTGA‑TGGTCATGCACTGAGCGATGAAGAAATTCGTTTCTTTATCAACGGCATTCGCGACAACACTATCTCCGAAGGGCAGATTGCCGCCCTCGCGATGACCAt                                                                                                                                                                                                  <  2:322482/251‑1 (MQ=255)                                      gCCTGATGCGCTGCGCTTAACAGGCCGACGAGACGTATCCACCCGTAGGCCGGATAAGGCGTAGACGCATCCGGCAAAAGCCGCCTCAGACTCTTTTCCTCGGGAGGGTACCTTGTTTCCCGCACAAGAAATTATTCGTAAAAAACGTGA‑TGGTCATGCACTGAGCGAGGAAGAAATTCGTTTCTTTATCAACGGCATTCGCGACAACACTATCTCCGAAGGGCAGATTGCCGCCCTCGCGATGACCAttt                                                                                                                                                                                                <  1:387145/251‑1 (MQ=255)                                        cTGATGCGCTGCGCTTATCAGGCCTACGAGACGTATCTACCCGTAGGCCGGATAAGGCGTAGACGCATCCGGCAAAAGCCGCCTCATACTCTTTTCCTCGGGAGGTTACCTTGTTTCTCGCACAAGAAATTATTCGTAAAAAACGTGA‑TGGTCATGCACTGAGCGATGAAGAAATTCGTTTCTTTATCAACGGCATTCGCGACAACACTATCTCCGAAGGGCAGATTGCCGCCCTCGCGATGACCATTttc                                                                                                                                                                                              >  1:274852/1‑251 (MQ=255)                                            tgcgctgcgcTTATCAGGCCTACGAGACGTATCTACCCGTAGTCCGGATAAGGCGTAGACGCATCCGGCAAAAGCCGCCTCATACTCTTTTCCTCGGGTGGTTACCTTGTTTCTCGCACAAGAAATTATTCGTAAAAACCGTGA‑TGGTCATGCACTGAGCGTTGAAGAAATTCGTTTCTTTATCAACGGCATTCGCGCCAACAATAGCTCCGAAGGGCAGATTGCCGCCCTCGTGATGTCCATTttcttac                                                                                                                                                                                          >  2:403568/1‑249 (MQ=255)                                            agcgctgcgctTATCAGGCCTACGAGACGTATCTACCCGTAGGCCGGATAAGGCGTCGACGCATACGGCAAAAGCCGCCTCATACTCTTTTCCTCGGGAGGTTACCTTGTTTCTCGCACAAGAAATTATTCGTAAAAAACGTGA‑TGGTCATGCACTGAGCGATGAAGCTATTCGTTTCTTTATCAAAGGCATTCGCGTCAACACTATCTCCGAAGGGCAGTTTGCCGCCCTCGCGATGTCAATTTTCTTcc                                                                                                                                                                                          >  2:43123/2‑251 (MQ=255)                                               gctgcgctTATCAGGCCTACGAGACGTATCTACCCGTAGGCCGGATAAGGCGTAGACGCATCCGGCAAAAGCCGCCTCATACTCTTTTCCTCGGGAGGTTACCTTGTTTCTCGCACAAGAAATTATTCGTAAAAAACGTGA‑TGGTCATGCACTGAGCGATGAAGAAATTCGTTTCTTTATCAACGGCATTCGCGACAACACTATCTCCGAAGGGCAGATTGCCGCCCTCGCGATGACCATTTTCTTCCACg                                                                                                                                                                                       <  1:301046/251‑1 (MQ=255)                                                                      aCGTATCTACCCGTAGGCCGGATAAGGCGTAGACGCATCCGGCAAAAGCCGCCTCATACTCTTTTCCTCGGGAGGTTACCTTGTTTCTCGCACAAGAAATTATTCGTAAAAAACGTGA‑TGGTCATGCACTGAGCGATGAAGAAATTCGTTTCTTTATCAACGGCATTCGCGACAACACTATCTCCGAAgg                                                                                                                                                                                                                             >  2:272949/1‑190 (MQ=255)                                                                      aCGTATCTACCCGTAGGCCGGATAAGGCGTAGACGCATCCGGCAAAAGCCGCCTCATACTCTTTTCCTCGGGAGGTTACCTTGTTTCTCGCACAAGAAATTATTCGTAAAAAACGTGA‑TGGTCATGCACTGAGCGATGAAGAAATTCGTTTCTTTATCAACGGCATTCGCGACAACACTATCTCCGAAgg                                                                                                                                                                                                                             <  1:272949/190‑1 (MQ=255)                                                                       cGTATCTACCCGGAGGCCGGATAAGGTGTCGACGCATCCGGCAAAAGCCGCCTCATACTCTTTTCCTCGGGAGGTTACCTTGTTTCTCGCACAAGAAATTATTCGTAAAAAACGTGA‑TGGTCATGCACTGAGCGATGAAGAAATTCGTTTCTTTATCAACGGCATTCGCGACAACACTATCTCCGAAGGGCAGATTGCCGCCCTCGCGATGACCATTTTCTTCCACGATATGACAATGCCTGAGCGTGtct                                                                                                                                                               <  2:118358/251‑1 (MQ=255)                                                                        gTATCTACCCGTAGGCCGGATAAGGCGTAGACGCATCCGGCAAAAGCCGCCTCATACTCTTTTCCTCGGGAGGTTACCTTGTTTCTCGCACAAGAAATTATTCGTAAAAAACGTGA‑TGGTCATGCACTGAGCGATGAAGAAATTCGTTTCTTTATCAACGGCATTCGCGACAACACTATCTCCGAAGGGCAGATTGCCGCCCTCGCGATGACCATTTTCTTCCACGATATGACAATGCCTGAGCGTGtctc                                                                                                                                                              <  2:123238/251‑1 (MQ=255)                                                                              aCCCGTAGGCCGGATAAGGCGTTGTCGCAGCCGGCAAAAGCCGCCTCATACTCTTTTCCTCGGGAGGGTACCTTGTTGCTCGCACAAGAAATTATTCGTAAAAAACGGGA‑TGGTCATGCACTGAGCGATGAAGAAATTCGTTTCTTTAGCAACGGCATTCGCGACAACACTATCTCCGAAGGGCAGAGTGCCGCCCTCGCGATGCCCATTTTCTTCCACGATATGACAATGCCTGTGCGTGTCTCGCTGAc                                                                                                                                                        <  2:472046/251‑1 (MQ=255)                                                                              aCCCGTAGGCCGGATAAGGCGTAGACGCATCCGGCAAAAGCCGCCTCATACTCTTTTCCTCGGGAGGTTACCTTGTTTCGCGCACAAGAAATTATTCGTAAAAAACGTGA‑TGGTCATGCACTGAGCGATGAAGAAATTCGTTTCTTTATCAACGGCATTCGCGACAACACTATCTCCGAAGGGCAGATTGCCGCCCTCGCGATGACCATTTTCTTCCACGATATGACAATGCCTGAGCGTGTCTCGCTg                                                                                                                                                          <  2:463547/249‑1 (MQ=255)                                                                                  gTAGGCCGGATAAGGCGTAGACGCATCCGGCAAAAGCCGCCTCATACTCTTTTCCTCGGGAGGTTACCTTGTTTCTCGCACAAGAAATTATTCGTAAAAAACGTGA‑TGGTCATGCACTGAGCGATGAAGAAATTCGTTTCTTTATCAACGGCATTCGCGACAACACTATCTCCGAAGGGCAGATTGCCGCCCTCGCGATGACCATTTTCTTCCACGATATGACAATGCCTGAGCGTGTCTCGCTGACCATg                                                                                                                                                    <  2:225637/251‑1 (MQ=255)                                                                                   tAGGCCGGATAAGGCGTAGACGCATCCGGCAAAAGCCGCCTCATACTCTTTTCCTCGGGAGGTTACCTTGTTTCTCGCACAAGAAATTATTCGTAAAAAACGTGA‑TGGTCATGCACTGAGCGATGAAGAAATTCGTTTCTTTATCAACGGCATTCGCGACAACACTATCTCCGAAGGGCAGATTGCCGCCCTCGCGATGACCATTTTCTTCCACGATATGACAATGCCTGAGCGTGTCTCGCTGACCATgg                                                                                                                                                   >  1:379233/1‑251 (MQ=255)                                                                                    aGGCCGGATAAGGCGTAGACGCATCCGGCAAAAGCCGTCTCATACTCATTTCCTCGTGAGGTTACCTTGTTTCTCGCACAAGAAATTATTCGTAAAAAACGTGA‑TGGTCATGCGCTGAGCGATGAAGAAATTCGTTTCTTTATCAACGGTATTCGCGACAACACTATCTCTGAAGGGCATATTGCCGCCCTCGCGATGACCATTTTCTTCCACGATATGACAATGCCTGAGCGTGTCTCGCTGACCATg                                                                                                                                                    <  1:246884/249‑1 (MQ=255)                                                                                      gCCGGATAAGGCGTAGACGCATCCGGCAAAGGCCGCCGCATACTCTTTTCCTCGGGAGGATAACTTGTTTCTCGCACAAGAAATTATTCGTAAAAAACGTGA‑TGGTCATGCCCTGAGCGATGAAGAAATTCGTTTCTTTATCAACGGCATTCGCGACAACACTATCTCCGAAGGGAAGATTGCCGCCCTCGCGATGACCATTTTCTTCCACGATATGACAATGCCTGAGCGGGACTCGCTgccaagtgcg                                                                                                                                                 >  2:119662/1‑241 (MQ=255)                                                                                             aaGGCGTAGACGCATCCGGCAAAAGCCGCCTCATACTCTTTTCCTCGGGAGGTTACCTTGTTTCTCGCACAAGAAATTATTCGTAAAAAACGTGA‑TGGTCATGCACTGAGCGATGAAGAAATTCGTTTCTTTATCAACGGCATTCGCGACAACACTATCTCCGAAGGGCAGATTGCCGCCCTCGCGATGACCATTTTCTTCCACGATATGACAATGCCTGAGCGTGTCTCGCTGACCATGGCGATGCgaga                                                                                                                                         <  2:259400/251‑1 (MQ=255)                                                                                               ggCGTAGACGCATCCGGCAAAAGCCGCCTCATACTCTTTTCCTCGGGAGGTTACCTTGTTTCTCGCACAAGAAATTATTCGTAAAAAACGTGA‑TGGTCATGCACTGAGCGATGAAGAAATTCGTTTCTTTATCAACGGCATTCGCGACAACACTATCTCCGAAGGGCAGATTGCCGCCCTCGCGATGACCATTTTCTTCCACGATATGACAATGCCTGAGCGTGTCTCGCTGCCCATGGCGATGCGAGAtt                                                                                                                                       >  2:146188/1‑251 (MQ=255)                                                                                                 cGTAGACGCATCCGGCAAAAGCCGCCTCATACTCTTTTCCTCGGGAGGTTACCTTGTTTCTCGCACAAGAAATTATTCGTAAAAAACGTGA‑TGGTCATGCACTGAGCGATGAAGAAATTCGTTTCTTTATCAACGGCATTCGCGACAACACTATCTCCGAAGGGCAGATTGCCGCCCTCGCGATGACCATTTTCTTCCACGATATGACAATGCCTGAGCGTGTCTCGCTGACCTTGGCGCTGCGAGATTc                                                                                                                                      >  2:94667/1‑250 (MQ=255)                                                                                                  gTAGACGCATCCGGCAAAAGCCGCCTCATACTCTTTTCCTCGGGAGGTTACCTTGTTTCTCGCACAAGAAATTATTCGTAAAAAACGTGA‑TGGTCATGCACTGAGCGATGAAGAAATTCGTTTCTTTATCAACGGCATTCGCGACAACACTATCTCCGAAGGGCAGATTGCCGCCCTCGCGATGACCATTTTCTTCCACGATATGACAATGCCTGAGCGTGTCTCGCTGACCATGGCGATGCGAGATTc                                                                                                                                      >  2:387279/1‑249 (MQ=255)                                                                                                                cAAAAGCCGCCTCATACTCTTTTCCTCGGGAGGTTACCTTGTTTCTCGCACAAGAAATTATTCGTAAAAAACGTGA‑TGGTCATGCACTGAGCGATGAAGAAATTCGTTTCTTTATCAACGGCATTCGCGACAACACTATCTCCGAAGGGCAGATTGCCGCCCTCGCGATGACCATTTTCTTCCACGATATGACAATGCCTGAGCGTGTCTCGCTGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGAc                                                                                                                      <  1:286514/251‑1 (MQ=255)                                                                                                                 aaaaGCCGCCTCATACTCTTTTCCTCGGGAGGTTACCTTGTTTCTCGCACAAGAAATTATTCGTAAAAAACGTGA‑TGGTCATGCACTGAGCGATGAAGAAATTCGTTTCTTTATCAACGGCATTCGCGACAACACTATCTCCGAAGGGCAGATTGCCGCCCTCGCGATGACCATTTTCTTCCACGATATGACAATGCCTGAGCGTGTCTCGCTGACCATGGCAATGCGAGATTCAGGAACCGTTCTCGACt                                                                                                                     >  1:480424/1‑251 (MQ=255)                                                                                                                 aaaaGCCGCCTCATACTCTGTTCCCCTGGAGGTTACCTTGTTTCTCGCACAAGAAATTATTCGTAAAAAACGTGA‑TGGTCATGCACTGAGCGATGAAGAAATTCGTTTCTTTATCAACGGCATTCGCGACAACACTATCTCCGAAGGGCAGATTGCCGCCCTCGCGATGACCAGTTTCTTCCGCGATATGACAATGCCTGAGCGTGTCTCGCTGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACt                                                                                                                     <  1:223471/251‑1 (MQ=255)                                                                                                                          cTCATACTCTTTTCCTCGGGAGGTTACCTTGTTTCTCGCACAAGACATTATTCGTAAAAAACGTGA‑TGGTCATGCACTGAGCGATGAAGAAATTCGTTTCTTTATCAACGGCATTCGCGACAACACTATCTCCGAAGGGCAGATTGCCGCCCTCGCGATGACCATTTTCTTCCACGATATGACAATGCCTGAGCGTGTCTCGCTGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGcc                                                                                                            >  1:381645/1‑251 (MQ=255)                                                                                                                                  ctTTTCCTCGGGAGGTTACCTTGTTTCTCGCACAAGAAATTATTCGTAAAAAACGTGA‑TGGTCATGCACTGAGCGATGAAGAAATTCGTTTCTTTATCAACGGCATTCGCGACAACACTATCTCCGAAGGGCAGATTGCCGCCCTCGCGATGACCATTTTCTTCCACGATATGACAATGCCTGAGCGTGTCTCGCTGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATc                                                                                                      <  1:387279/249‑1 (MQ=255)                                                                                                                                  ctTTTCCTCGGGAGGTTACCTTGTTTCTCGCACAAGAAATTATTCGTAAAAAACGTGA‑TGGTCATGCACTGAGCGATGAAGAAATTCGTTTCTTTATCAACGGCATTCGCGACAACACTATCTCCGAAGGGCAGATTGCCGCCCTCGCGATGACCATTTTCTTCCACGATATGACAATGCCTGAGCGTGTCTCGCTGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTg                                                                                                    >  1:344859/1‑251 (MQ=255)                                                                                                                                    tttCCTCGGGAGGTTACCTTGTTTCTCGCCAAAGAAATTATTCGTAAAAAACGTGAGTGGT‑ATGCACTGAGCGATGAAGAAATTCGTTTCTTTATCAACGGCATGCGAGACAACACTATCTCCGAAGTGCAGATTGCCGCCCTTGCGATGACCATGTTCTTCCACGATATGACAATGCCTGAGCGTGTCTCGCTGACCATGGCGATGCGGGATTTAGGAACCGTTCTCGACTGTAAAAGCCTGCATCTGa                                                                                                   <  2:296078/250‑1 (MQ=255)                                                                                                                                                        gTTTCTCGCACAAGAAATTATTCGTAAAAAACGTGA‑TGGTCATGCACTGAGCGATGAAGAAATTCGTTTCTTTATCAACGGCATTCGCGACAACACTATCTCCGAAGGGCAGATTGCCGCCCTCGCGATGACCATTTTCTTCCACGATATGACAATGCCTGAGCGTGTCTCGCTGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATaaa                                                                               <  1:146188/250‑1 (MQ=255)                                                                                                                                                                     gAAATTATTCGTAAAAAACGTGA‑TGGTCATGCACTGAGCGATGAAGAAATTCGTTTCTTTATCAACGGCATTCGCGACAACACTATCTCCGAAGGGCAGATTGCCGCCCTCGCGATGACCATTTTCTTCCACGATATGACAATGCCTGAGCGTGTCTCGCTGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACtggtgg                                                                 <  1:311109/251‑1 (MQ=255)                                                                                                                                                                             tCGTAAAAAACGTGA‑TGGTCATGCACTGAGCGATGAAGAAATTCGTTTCTTTATCAACGGCATTCGCGACAACACTATCTCCGAAGGGCAGATTGCCGCCCTCGCGATGACCATTTTCTTCCACGATATGACAATGCCTGAGCGTGTCTCGCTGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCg                                                         >  1:409343/1‑251 (MQ=255)                                                                                                                                                                                 aaaaaaCGTGA‑TGGTCATGCACTGAGCGATGAAGAAATTCGTTTCTTTATCAACGGCATTCGCGACAACACTATCTCCGAAGGGCAGATTGCCGCCCTCGCGATGACCATTTTCTTCCACGATATGACAATGCCTGAGCGTGTCTCGCTGACCATGGCGATGCGAGACTCAGGAACCGTTCTCGCCTGGAAAAGCCTGCATCTGAATGGCCCGCTTGTTGATAAACATTCCACTGGTGGCGTGGGCGAtgg                                                     >  1:379735/1‑250 (MQ=255)                                                                                                                                                                                   aaaaCGTGA‑TGGTCATGCACTGAGCGATGAAGAAATTCGTTTCTTTATCAACGGCATTCGCGACAACACTATCTCCGAAGGGCAGATTGCCGCCCTCGCGATGACCATTTTCTTCCACGATATGACAATGCCTGAGCGTGTCTCGCTGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGAtgtg                                                    >  1:290734/1‑250 (MQ=255)                                                                                                                                                                                                 cATGCACTGAGCGATGAAGAAATTCGTTTCTTTATCAACGGCATTCGCGACAACACTATCTCCGAAGGGCAGATTGCCGCCCTCGCGATGACCATTTTCTTCCACGATATGACAATGCCTGAGCGTGTCTCGCTGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGtt                                      >  1:193234/1‑251 (MQ=255)                                                                                                                                                                                                    gctcggAGCGATGAAGAAATTCGTTTCTTTATCAACGGCACTCGCGACAACACTATCTCCGAAGGGCAGATTGCCGCCCTCGCGATGACCATTTTCTTCCACGATATGACAATGCCTGAGCGTGTCTCGCTGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGGTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTgg                                    <  2:346753/245‑1 (MQ=255)                                                                                                                                                                                                        tGAGCGCTGAAGAAATTAGTTACTTTATCTACGGCATTAGCGACAACACTATCTCAGAAGGGCAGATTGCCGCCCTCGCGATGCCCATTTTCTTACACGATATGACAATGCCTGAGCGTGTCTCGCTGACCATGGCTATGCGAGCTTCAGGAACCGTTCTCGACTGGAAAAGCCTGAATCTGAATGGCCCGATTGTTGATAAACATTACCCTGGAGGCGTGGGCGATATGACTTCGCAGTTGTTggagc                                 >  2:284241/1‑246 (MQ=255)                                                                                                                                                                                                                  agaaATTCGTTTCTTTATCAACGGCATTCGCGACAACACTATCTCCGAAGGGCAGATTGCCGCCCTCGCGATGACCATTTTCTTCCACGATATGACAATGCCTGAGCGTGTCTCGCTGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAg                     <  1:416897/251‑1 (MQ=255)                                                                                                                                                                                                                    aaaTTCGTTTCTTTAACAACGGCATTCGCGACAACACTATCTCCGAAGGGCAGATTGCCGCCCTCGCGATGACCATTTTCTTCCACGATATGACAATGCCTGAGCGTGTCTCGCTGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGcc                   <  1:433874/251‑1 (MQ=255)                                                                                                                                                                                                                            ttCTTTATCAACGGCATTCGCGACAACACTATCTCCGAAGGGCAGATTGCCGCCCTCGCGATGACCATTTTCTTCCACGATATGACAATGCCTGAGCGTGTCTCGCTGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGCGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGcggcgg           >  2:424468/1‑251 (MQ=255)                                                                                                                                                                                                                             tCTTTATCAACGGCATTCGCGACAACACTATCTCCGACGGGCAGATTGCCGCCCTCGCGATGACCATTTTCTTCCACGATATGACAATGCCTGAGCGTGTCTCGCTGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGCTAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCggcggc          >  2:66913/1‑251 (MQ=255)                                                                                                                                                                                                                               tttATCAACGGCATTCGCGACAACACTATCTCCGAAGGGCAGATTGCCGCCCTCGCGATGACCATTTTCTTCCACGATATGACAATGCCTGAGCGTGTCTCGCTGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGcggcgggc         >  2:365809/1‑248 (MQ=255)                                                                                                                                                                                                                                     aaCGGCATTCGCGACAACACTATCTCCGAAGGGCAGATTGCCGCCCTCGCGATGACCATTTTCTTCCACGATATGACAATGCCTGAGCGTGTCTCGCTGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGCCTTCGATGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTcc  >  1:468739/1‑251 (MQ=255)                                                                                                                                                                                                                                     aaCGGCATTCGCGAAAACACTATCTCCGAAGGGCAGATTGCCGCCCTCGCGATGACCATTTTCTTCCACGATATGACAATGCCTGAGCGTGTCTCGCTGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTcc  <  1:246726/251‑1 (MQ=255)                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                      GTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATCAGGCCTACGAGACGTATCTACCCGTAGGCCGGATAAGGCGTAGACGCATCCGGCAAAAGCCGCCTCATACTCTTTTCCTCGGGAGGTTACCTTGTTTCTCGCACAAGAAATTATTCGTAAAAAACGTGA‑TGGTCATGCGCTGAGCGATGAAGAAATTCGTTTCTTTATCAACGGTATTCGCGACAACACTATCTCCGAAGGGCAGATTGCCGCCCTCGCGATGACCATTTTCTTCCACGATATGACAATGCCTGAGCGTGTCTCGCTGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACACTCCACCGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTCC  >  NC_000913/4618080‑4618557

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑