Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,618,276	G→A	A16A (GCG→GCA)	deoA →	thymidine phosphorylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,618,276	0	G	A	98.1%	163.8 / ‑4.6	55	A16A (GCG→GCA)	deoA	thymidine phosphorylase
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base A (24/28);  total (26/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.65e-01

TCCAGCCTGCTGGCAAGCCTGCTGAAAGCGCTGGGTCACGGCGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATCAGGCCTACGAGACGTATCTACCCGTAGGCCGGATAAGGCGTAGACGCATCCGGCAAAAGCCGCCTCATACTCTTTTCCTCGGGAGGTTACCTTGTTTCTCGCACAAGAAATTATTCGTAAAAAACGTGA‑TGGTCATGCGCTGAGCGATGAAGAAATTCGTTTCTTTATCAACGGTATTCGCGACAACACTATCTCCGAAGGGCAGATTGCCGCCCTCGCGATGACCATTTTCTTCCACGATATGACAATGCCTGAGCGTGTCTCGCTGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACACTCCACCGGTGGCGTCGGCGATGTGACTTCGCTGATGTT  >  NC_000913/4618034‑4618521                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                      tCCAGCCTGCTGGCAAGCCTGTTGAAAGCGCTGGGCCACGGTGACGGTAAGAGCGCCAGCAGCTACTAAGGAAGATGCTTTACGCCTGATGCGCTGCGCTTATCAGGCCTACGAGACGTATCTACCCGTAGGCCGGATAAGGCGTAGACGCATCCGGCAAAAGCCGCCTCATACTCTTTTCCTCGGGAGGTTACCTTGTTTCTCGCACAAGAAATTATTCGTAAAAAACGTGA‑TGGTCATGCACTGAGCg                                                                                                                                                                                                                                                <  2:476216/250‑1 (MQ=255)       ctgctgGCAAGCCTGTTGAAAGCGCTGGGCCACGGTGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATCAGGCCTACGAGACGTATCTACCCGTAGGCCGGATAAGGCGTAGACGCATCCGGCAAAAGCCGCCTCATACTCTTTTCCTCGGGAGGTTACCTTGTTTCTCGCACAAGAAATTATTCGTAAAAAACGTGA‑TGGTCATGCACTGAGCGATga                                                                                                                                                                                                                                            >  1:225637/1‑248 (MQ=255)               aaGCCTGTTGAAAGCGCTGGGCCACGGGGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATCAGGCCTACGAGACGTATCTACCCGTAGGCCGGATAAGGCGTAGACGCATCCGGCAAAAGCCGCCTCATACTCTTTTCCTCGGGAGGTTACCTTGTTTCTCGCACAAGAAATTATTCGTAAAAAACGTGA‑TGGTCATGCACTGAGCGATGAAGAAATTCGtt                                                                                                                                                                                                                                 <  2:147925/251‑1 (MQ=255)                 gCCTGTTGAAAGCGCTGGGCCACGGTGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATCAGGCCTACGAGACGTATCTACCCGTAGGCCGGATAAGGCGTAGACGCATCCGGCAAAAGCCGCCTCATACTCTTTTCCTCGGGAGGTTACCTTGTTTCTCGCACAAGAAATTATTCGTAAAAAACGTGA‑TGGTCATGCACTGAGCGATGAAGAAATTCGTTTc                                                                                                                                                                                                                               >  1:259400/1‑251 (MQ=255)                  ccTGTTGAAAGCGCTGGGCCACGGTGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATCAGGCCTACGAGACGTATCTACCCGTAGGCCGGATAAGGCGTAGACGCATCCGGCAAAAGCCGCCTCATACTCTTTTCCTCGGGAGGTTACCTTGTTTCTCGCACAAGAAATTATTCGTAAAAAACGTGA‑TGGTCATGCACTGAGCGATGAAGAAATTCGTTTCt                                                                                                                                                                                                                              <  1:102320/251‑1 (MQ=255)                   ctgctgACCGCGCTGAATCACCGCGAAGGTAAGCGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCAGATGCGCTGCGCTTATCAGGCCTACGAGACGTATCTACCAGTAGGCCGGGTAAGGCGTAGACGCATCCGGCAAAAGCCGCCTCATACTCTTTTCATCGGGAGGTTACCTTGTTTCTCGCACAAGAAATTATTCGTAAAAAACGGGA‑TGGTCATGTTCTGAGCGATGTAGAAATTCGATTCtt                                                                                                                                                                                                                             >  2:246884/1‑251 (MQ=255)                               cTGGGACACGGTGCCGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATCAGGCCTACGAGACGTATCTACCCGTAGGCCGGATAAGGCGTAGACGCATCCGGCAAAAGCCGCCTCATACTCTTTTCCTCGGGAGGTTACCTTGTTTCTCGCACAAGAAATTATTCGTAAAAAACGTGA‑TGGTCATGCACTGAGAGATGAAGAAATTCGTTTCTTTATCAACggcat                                                                                                                                                                                                                 >  2:377569/1‑248 (MQ=255)                                               gtaggagCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATCAGGCCTACGAGACGTATCTACCCGTAGGCCGGATCAGGCGTAGACGCATCCGGCAAAAGCCGCCTCATACTCTTTTCCTCGGGAGGTTACCTTGTTTCTCGCACAAGAAATTATTCGTAACAAACGTGA‑TGGTCATGCACTGAGCGATGAAGAAATTCGTTACTTTATCATCGGCATTCGCGCCAACACTAtc                                                                                                                                                                                                 >  2:383922/5‑251 (MQ=255)                                               ggaaGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATCAGGCCTACGAGACGTATCTACCCGTAGGCCGGATAAGGCGTAGACGCATCCGGCAAAAGCCGCCTCATACTCTGTTCCTCGGGAGGTTACCTTGTTTCTCGCACAAGAAATTATTCGTAAAAAACGTGA‑TGGTCATGCACGGAGCGATGAAGAAATTCGTTTCTTTATCAACGGCATTCGCGACAACACTAtc                                                                                                                                                                                                 <  2:211698/249‑1 (MQ=255)                                                 aaGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATCAGGCCTACGAGACGTATCTACCCGTAGGCCGGATAAGGCGTAGACGCATCCGGCAAAAGCCGCCTCATACTCTTTTCCTCGGGAGGTTACCTTGTTTCTCGCACAAGAAATTATTCGTAAAAAACGTGA‑TGGTCATGCACTGAGCGATGAAGAAATTCGTTTCTTTATCAACGGCATTCGCGACAACACTAtctc                                                                                                                                                                                               >  1:164602/1‑251 (MQ=255)                                                 aaGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATCAGGCCTACGAGACGTATCTACCCGTAGGCCGGATAAGGCGTAGACGCATCCGGCAAAAGCCGCCTCATACTCTTTTCCTCGGGAGGTTACCTTGTTTCTCGCACAAGAAATTATTCGTAAAAAACGTGA‑TGGTCATGCACTGAGCGATGAAGAAATTCGTTTCTTTATCAACGGCATTCGCGACAACACTAtctc                                                                                                                                                                                               <  1:39413/251‑1 (MQ=255)                                                 aaGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATCAGGCCTACGAGACGTATCTACCCGTAGGCCGGATAAGGCGTAGACGCATCCGGCAAAAGCCGCCTCATACTCTTTTCCTCGGGAGGTTACCTTGTTTCTCGCACAAGAAATTATTCGTAAAAAACGTGA‑TGGTCATGCACTGAGCGATGAAGAAATTCGTTTCTTTATCAACGGCATTCGCGACAACACTAtct                                                                                                                                                                                                <  1:123714/250‑1 (MQ=255)                                                    agCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATCAGGCCTACGAGACGTATCTACCCGTAGGCCGGATAAGGCGTAGACGCATCCGGCAAAAGCCGCCTCATACTCTTTTCCTCGGGAGGTTACCTTGTTTCTCGCACAAGAAATTATTCGTAAAAAACGTGA‑TGGTCATGCACTGAGCGATGAAGAAATTCGTTTCTTTATCAACGGCATTCGCGACAACACTATCTCCg                                                                                                                                                                                             >  1:322482/1‑250 (MQ=255)                                                           gcagcTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATCAGGCCTACGAGACGTATCTACCCGTAGGCCGGATAAGGCGTAGACGCATCCGGCAAAAGCCGCCTCATACTCTTTTCCTCGGGAGGTTACCTTGTTTCTCGCACAAGAAATTATTCGTAAAAAACGTGA‑TGGTCATGCACTGAGCGATGAAGAAATTCGTTTCTTTATCAACGGCAtt                                                                                                                                                                                                                <  1:88306/224‑1 (MQ=255)                                                           gcagcTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATCAGGCCTACGAGACGTATCTACCCGTAGGCCGGATAAGGCGTAGACGCATCCGGCAAAAGCCGCCTCATACTCTTTTCCTCGGGAGGTTACCTTGTTTCTCGCACAAGAAATTATTCGTAAAAAACGTGA‑TGGTCATGCACTGAGCGATGAAGAAATTCGTTTCTTTATCAACGGCAtt                                                                                                                                                                                                                >  2:88306/1‑224 (MQ=255)                                                              gcTACCAAGTAAGATGTGTTACGCCTGATGTGCTGCGCTTATCAGGCCTACGAGACGTATCTACCCGTAGGCCGGATAAGGCGTAGACGCATCCGGCAAAAGCCGCCTCATACTCTTTTCCTCGGGAGGTTACCTTGTTTCTCGCACAAGAAATTATTCGTAAAAAACGTGA‑TGGTCATGCACTGAGCGATGAAGAAATTCGTTTCTTTATCAACGGCATTCGCGACAACACTATCTCCGAAGGGCAGAtt                                                                                                                                                                                  <  2:161187/251‑1 (MQ=255)                                                                        aagATGCTTTACGCCTGATGCGCTGCGCTTATCAGGCCTACGAGACGTATCTACCCGTAGGCCGGATAAGGCGTAGACGCATCCGGCAAAAGCCGCCTCATACTCTTTTCCTCGGGAGGTTACCTTGTTTCTCGCACAAGAAATTATTCGTAAAAAACGTGA‑TGGTCATGCACTGAGCGATGAAGAAATTCGTTTCTTTATCAACGGCATTCGCGACAACACTATCTCCGAAGGGCAGATTGCCGCCCTcg                                                                                                                                                                        <  2:164602/251‑1 (MQ=255)                                                                          gATGCTTTACGCCTGATGCGCTGCGCTTATCAGGCCTACGAGACGTATCTACCCGTAGGCCGGATAAGGCGTAGACGCATCCGGCAAAAGCCGCCTCATACTCTTTTCCTCGGGAGGTTACCTTGTTTCTCGCACAAGAAATTATTCGTAAAAAACGTGA‑TGGTCATGCACTGAGCGATGAAGAAATTCGTTTCTTTATCAACGGCATTCGCGACAACACTATCTCCGAAGGGCAGATTGCCGCCCTcgcg                                                                                                                                                                      >  1:304476/1‑251 (MQ=255)                                                                                 tACGCCTGATGCGCTGCGCTTATCAGGCCTACGAGACGTATCTACCCGTAGGCCGGATAAGGCGTAGACGCATCCGGCAAAAGCCGCCTCATACTCTTTTCCTCGGGAGGTTACCTTGTTTCTCGCACAAGAAATTATTCGTAAAAAACGTGA‑TGGTCATGCACTGAGCGATGAAGAAATTCGTTTCTTTATCAACGGCATTCGCGACAACACTATCTCCGAAGGGCAGATTGCCGCCCTCGCGATGAcc                                                                                                                                                                <  2:202576/250‑1 (MQ=255)                                                                                  aCGCCTGATGCGCTGCGCTTATCAGGCCTACGAGACGTATCTACCCGTAGGCCGGATAAGGCGTAGACGCATCCGGCAAAAGCCGCCGCATACTCTTTTCCTCGGGAGGTTACCTTGTTTCTCGCACAAGAAATTATTCGTAAAAAACGTGA‑TGGTCATGCACTGAGCGATGAAGAAATTCGTTTCTTTATCAACGGCATTCGCGACAACACTATCTCCGAAGGGCAGATTGCCGCCCTCGCGATGACCAt                                                                                                                                                              <  1:294069/251‑1 (MQ=255)                                                                                  aCGCCTGATGCGCGGCGCTTATCAGGCCTACGAGACGTAGCTACCCGTAGGCCGGATAAGGCGTAGACGCATCCGGCAAAAGCCGCCTCATACTCTTTTCCTCGGGAGGTTACCTTGTTTCTCGCACAAGAAATTATTCGTAAAAAACGTGA‑TGGTCATGCACTGAGCGATGAAGAAATTCGTTTCTTTATCAACGGCATTCGCGACAACACTATCTCCGAAGGGCAGATTGCCGCCCTCGCGATGACCAt                                                                                                                                                              <  2:322482/251‑1 (MQ=255)                                                                                    gCCTGATGCGCTGCGCTTAACAGGCCGACGAGACGTATCCACCCGTAGGCCGGATAAGGCGTAGACGCATCCGGCAAAAGCCGCCTCAGACTCTTTTCCTCGGGAGGGTACCTTGTTTCCCGCACAAGAAATTATTCGTAAAAAACGTGA‑TGGTCATGCACTGAGCGAGGAAGAAATTCGTTTCTTTATCAACGGCATTCGCGACAACACTATCTCCGAAGGGCAGATTGCCGCCCTCGCGATGACCAttt                                                                                                                                                            <  1:387145/251‑1 (MQ=255)                                                                                      cTGATGCGCTGCGCTTATCAGGCCTACGAGACGTATCTACCCGTAGGCCGGATAAGGCGTAGACGCATCCGGCAAAAGCCGCCTCATACTCTTTTCCTCGGGAGGTTACCTTGTTTCTCGCACAAGAAATTATTCGTAAAAAACGTGA‑TGGTCATGCACTGAGCGATGAAGAAATTCGTTTCTTTATCAACGGCATTCGCGACAACACTATCTCCGAAGGGCAGATTGCCGCCCTCGCGATGACCATTttc                                                                                                                                                          >  1:274852/1‑251 (MQ=255)                                                                                          tgcgctgcgcTTATCAGGCCTACGAGACGTATCTACCCGTAGTCCGGATAAGGCGTAGACGCATCCGGCAAAAGCCGCCTCATACTCTTTTCCTCGGGTGGTTACCTTGTTTCTCGCACAAGAAATTATTCGTAAAAACCGTGA‑TGGTCATGCACTGAGCGTTGAAGAAATTCGTTTCTTTATCAACGGCATTCGCGCCAACAATAGCTCCGAAGGGCAGATTGCCGCCCTCGTGATGTCCATTttcttac                                                                                                                                                      >  2:403568/1‑249 (MQ=255)                                                                                          agcgctgcgctTATCAGGCCTACGAGACGTATCTACCCGTAGGCCGGATAAGGCGTCGACGCATACGGCAAAAGCCGCCTCATACTCTTTTCCTCGGGAGGTTACCTTGTTTCTCGCACAAGAAATTATTCGTAAAAAACGTGA‑TGGTCATGCACTGAGCGATGAAGCTATTCGTTTCTTTATCAAAGGCATTCGCGTCAACACTATCTCCGAAGGGCAGTTTGCCGCCCTCGCGATGTCAATTTTCTTcc                                                                                                                                                      >  2:43123/2‑251 (MQ=255)                                                                                             gctgcgctTATCAGGCCTACGAGACGTATCTACCCGTAGGCCGGATAAGGCGTAGACGCATCCGGCAAAAGCCGCCTCATACTCTTTTCCTCGGGAGGTTACCTTGTTTCTCGCACAAGAAATTATTCGTAAAAAACGTGA‑TGGTCATGCACTGAGCGATGAAGAAATTCGTTTCTTTATCAACGGCATTCGCGACAACACTATCTCCGAAGGGCAGATTGCCGCCCTCGCGATGACCATTTTCTTCCACg                                                                                                                                                   <  1:301046/251‑1 (MQ=255)                                                                                                         aGGCCTACGAGACGTATCTACCCGTAGGCCGGATAAGGCGTAGACGCATCCGGCAAAAGCCGCCTCATACTCTTTTCCTCGGGAGGTTACCTTGTTTCTCGCACAAGAAATTATTCGTAAAAAACGTGA‑TGGTCATGCACTGAGCGATGAAGaaa                                                                                                                                                                                                                                       >  2:377373/1‑155 (MQ=255)                                                                                                         aGGCCTACGAGACGTATCTACCCGTAGGCCGGATAAGGCGTAGACGCATCCGGCAAAAGCCGCCTCATACTCTTTTCCTCGGGAGGTTACCTTGTTTCTCGCACAAGAAATTATTCGTAAAAAACGTGA‑TGGTCATGCACTGAGCGATGAAGaaa                                                                                                                                                                                                                                       <  1:377373/155‑1 (MQ=255)                                                                                                                    aCGTATCTACCCGTAGGCCGGATAAGGCGTAGACGCATCCGGCAAAAGCCGCCTCATACTCTTTTCCTCGGGAGGTTACCTTGTTTCTCGCACAAGAAATTATTCGTAAAAAACGTGA‑TGGTCATGCACTGAGCGATGAAGAAATTCGTTTCTTTATCAACGGCATTCGCGACAACACTATCTCCGAAgg                                                                                                                                                                                         >  2:272949/1‑190 (MQ=255)                                                                                                                    aCGTATCTACCCGTAGGCCGGATAAGGCGTAGACGCATCCGGCAAAAGCCGCCTCATACTCTTTTCCTCGGGAGGTTACCTTGTTTCTCGCACAAGAAATTATTCGTAAAAAACGTGA‑TGGTCATGCACTGAGCGATGAAGAAATTCGTTTCTTTATCAACGGCATTCGCGACAACACTATCTCCGAAgg                                                                                                                                                                                         <  1:272949/190‑1 (MQ=255)                                                                                                                     cGTATCTACCCGGAGGCCGGATAAGGTGTCGACGCATCCGGCAAAAGCCGCCTCATACTCTTTTCCTCGGGAGGTTACCTTGTTTCTCGCACAAGAAATTATTCGTAAAAAACGTGA‑TGGTCATGCACTGAGCGATGAAGAAATTCGTTTCTTTATCAACGGCATTCGCGACAACACTATCTCCGAAGGGCAGATTGCCGCCCTCGCGATGACCATTTTCTTCCACGATATGACAATGCCTGAGCGTGtct                                                                                                                           <  2:118358/251‑1 (MQ=255)                                                                                                                      gTATCTACCCGTAGGCCGGATAAGGCGTAGACGCATCCGGCAAAAGCCGCCTCATACTCTTTTCCTCGGGAGGTTACCTTGTTTCTCGCACAAGAAATTATTCGTAAAAAACGTGA‑TGGTCATGCACTGAGCGATGAAGAAATTCGTTTCTTTATCAACGGCATTCGCGACAACACTATCTCCGAAGGGCAGATTGCCGCCCTCGCGATGACCATTTTCTTCCACGATATGACAATGCCTGAGCGTGtctc                                                                                                                          <  2:123238/251‑1 (MQ=255)                                                                                                                            aCCCGTAGGCCGGATAAGGCGTTGTCGCAGCCGGCAAAAGCCGCCTCATACTCTTTTCCTCGGGAGGGTACCTTGTTGCTCGCACAAGAAATTATTCGTAAAAAACGGGA‑TGGTCATGCACTGAGCGATGAAGAAATTCGTTTCTTTAGCAACGGCATTCGCGACAACACTATCTCCGAAGGGCAGAGTGCCGCCCTCGCGATGCCCATTTTCTTCCACGATATGACAATGCCTGTGCGTGTCTCGCTGAc                                                                                                                    <  2:472046/251‑1 (MQ=255)                                                                                                                            aCCCGTAGGCCGGATAAGGCGTAGACGCATCCGGCAAAAGCCGCCTCATACTCTTTTCCTCGGGAGGTTACCTTGTTTCGCGCACAAGAAATTATTCGTAAAAAACGTGA‑TGGTCATGCACTGAGCGATGAAGAAATTCGTTTCTTTATCAACGGCATTCGCGACAACACTATCTCCGAAGGGCAGATTGCCGCCCTCGCGATGACCATTTTCTTCCACGATATGACAATGCCTGAGCGTGTCTCGCTg                                                                                                                      <  2:463547/249‑1 (MQ=255)                                                                                                                                gTAGGCCGGATAAGGCGTAGACGCATCCGGCAAAAGCCGCCTCATACTCTTTTCCTCGGGAGGTTACCTTGTTTCTCGCACAAGAAATTATTCGTAAAAAACGTGA‑TGGTCATGCACTGAGCGATGAAGAAATTCGTTTCTTTATCAACGGCATTCGCGACAACACTATCTCCGAAGGGCAGATTGCCGCCCTCGCGATGACCATTTTCTTCCACGATATGACAATGCCTGAGCGTGTCTCGCTGACCATg                                                                                                                <  2:225637/251‑1 (MQ=255)                                                                                                                                 tAGGCCGGATAAGGCGTAGACGCATCCGGCAAAAGCCGCCTCATACTCTTTTCCTCGGGAGGTTACCTTGTTTCTCGCACAAGAAATTATTCGTAAAAAACGTGA‑TGGTCATGCACTGAGCGATGAAGAAATTCGTTTCTTTATCAACGGCATTCGCGACAACACTATCTCCGAAGGGCAGATTGCCGCCCTCGCGATGACCATTTTCTTCCACGATATGACAATGCCTGAGCGTGTCTCGCTGACCATgg                                                                                                               >  1:379233/1‑251 (MQ=255)                                                                                                                                  aGGCCGGATAAGGCGTAGACGCATCCGGCAAAAGCCGTCTCATACTCATTTCCTCGTGAGGTTACCTTGTTTCTCGCACAAGAAATTATTCGTAAAAAACGTGA‑TGGTCATGCGCTGAGCGATGAAGAAATTCGTTTCTTTATCAACGGTATTCGCGACAACACTATCTCTGAAGGGCATATTGCCGCCCTCGCGATGACCATTTTCTTCCACGATATGACAATGCCTGAGCGTGTCTCGCTGACCATg                                                                                                                <  1:246884/249‑1 (MQ=255)                                                                                                                                    gCCGGATAAGGCGTAGACGCATCCGGCAAAGGCCGCCGCATACTCTTTTCCTCGGGAGGATAACTTGTTTCTCGCACAAGAAATTATTCGTAAAAAACGTGA‑TGGTCATGCCCTGAGCGATGAAGAAATTCGTTTCTTTATCAACGGCATTCGCGACAACACTATCTCCGAAGGGAAGATTGCCGCCCTCGCGATGACCATTTTCTTCCACGATATGACAATGCCTGAGCGGGACTCGCTgccaagtgcg                                                                                                             >  2:119662/1‑241 (MQ=255)                                                                                                                                           aaGGCGTAGACGCATCCGGCAAAAGCCGCCTCATACTCTTTTCCTCGGGAGGTTACCTTGTTTCTCGCACAAGAAATTATTCGTAAAAAACGTGA‑TGGTCATGCACTGAGCGATGAAGAAATTCGTTTCTTTATCAACGGCATTCGCGACAACACTATCTCCGAAGGGCAGATTGCCGCCCTCGCGATGACCATTTTCTTCCACGATATGACAATGCCTGAGCGTGTCTCGCTGACCATGGCGATGCgaga                                                                                                     <  2:259400/251‑1 (MQ=255)                                                                                                                                             ggCGTAGACGCATCCGGCAAAAGCCGCCTCATACTCTTTTCCTCGGGAGGTTACCTTGTTTCTCGCACAAGAAATTATTCGTAAAAAACGTGA‑TGGTCATGCACTGAGCGATGAAGAAATTCGTTTCTTTATCAACGGCATTCGCGACAACACTATCTCCGAAGGGCAGATTGCCGCCCTCGCGATGACCATTTTCTTCCACGATATGACAATGCCTGAGCGTGTCTCGCTGCCCATGGCGATGCGAGAtt                                                                                                   >  2:146188/1‑251 (MQ=255)                                                                                                                                               cGTAGACGCATCCGGCAAAAGCCGCCTCATACTCTTTTCCTCGGGAGGTTACCTTGTTTCTCGCACAAGAAATTATTCGTAAAAAACGTGA‑TGGTCATGCACTGAGCGATGAAGAAATTCGTTTCTTTATCAACGGCATTCGCGACAACACTATCTCCGAAGGGCAGATTGCCGCCCTCGCGATGACCATTTTCTTCCACGATATGACAATGCCTGAGCGTGTCTCGCTGACCTTGGCGCTGCGAGATTc                                                                                                  >  2:94667/1‑250 (MQ=255)                                                                                                                                                gTAGACGCATCCGGCAAAAGCCGCCTCATACTCTTTTCCTCGGGAGGTTACCTTGTTTCTCGCACAAGAAATTATTCGTAAAAAACGTGA‑TGGTCATGCACTGAGCGATGAAGAAATTCGTTTCTTTATCAACGGCATTCGCGACAACACTATCTCCGAAGGGCAGATTGCCGCCCTCGCGATGACCATTTTCTTCCACGATATGACAATGCCTGAGCGTGTCTCGCTGACCATGGCGATGCGAGATTc                                                                                                  >  2:387279/1‑249 (MQ=255)                                                                                                                                                              cAAAAGCCGCCTCATACTCTTTTCCTCGGGAGGTTACCTTGTTTCTCGCACAAGAAATTATTCGTAAAAAACGTGA‑TGGTCATGCACTGAGCGATGAAGAAATTCGTTTCTTTATCAACGGCATTCGCGACAACACTATCTCCGAAGGGCAGATTGCCGCCCTCGCGATGACCATTTTCTTCCACGATATGACAATGCCTGAGCGTGTCTCGCTGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGAc                                                                                  <  1:286514/251‑1 (MQ=255)                                                                                                                                                               aaaaGCCGCCTCATACTCTTTTCCTCGGGAGGTTACCTTGTTTCTCGCACAAGAAATTATTCGTAAAAAACGTGA‑TGGTCATGCACTGAGCGATGAAGAAATTCGTTTCTTTATCAACGGCATTCGCGACAACACTATCTCCGAAGGGCAGATTGCCGCCCTCGCGATGACCATTTTCTTCCACGATATGACAATGCCTGAGCGTGTCTCGCTGACCATGGCAATGCGAGATTCAGGAACCGTTCTCGACt                                                                                 >  1:480424/1‑251 (MQ=255)                                                                                                                                                               aaaaGCCGCCTCATACTCTGTTCCCCTGGAGGTTACCTTGTTTCTCGCACAAGAAATTATTCGTAAAAAACGTGA‑TGGTCATGCACTGAGCGATGAAGAAATTCGTTTCTTTATCAACGGCATTCGCGACAACACTATCTCCGAAGGGCAGATTGCCGCCCTCGCGATGACCAGTTTCTTCCGCGATATGACAATGCCTGAGCGTGTCTCGCTGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACt                                                                                 <  1:223471/251‑1 (MQ=255)                                                                                                                                                                        cTCATACTCTTTTCCTCGGGAGGTTACCTTGTTTCTCGCACAAGACATTATTCGTAAAAAACGTGA‑TGGTCATGCACTGAGCGATGAAGAAATTCGTTTCTTTATCAACGGCATTCGCGACAACACTATCTCCGAAGGGCAGATTGCCGCCCTCGCGATGACCATTTTCTTCCACGATATGACAATGCCTGAGCGTGTCTCGCTGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGcc                                                                        >  1:381645/1‑251 (MQ=255)                                                                                                                                                                                ctTTTCCTCGGGAGGTTACCTTGTTTCTCGCACAAGAAATTATTCGTAAAAAACGTGA‑TGGTCATGCACTGAGCGATGAAGAAATTCGTTTCTTTATCAACGGCATTCGCGACAACACTATCTCCGAAGGGCAGATTGCCGCCCTCGCGATGACCATTTTCTTCCACGATATGACAATGCCTGAGCGTGTCTCGCTGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATc                                                                  <  1:387279/249‑1 (MQ=255)                                                                                                                                                                                ctTTTCCTCGGGAGGTTACCTTGTTTCTCGCACAAGAAATTATTCGTAAAAAACGTGA‑TGGTCATGCACTGAGCGATGAAGAAATTCGTTTCTTTATCAACGGCATTCGCGACAACACTATCTCCGAAGGGCAGATTGCCGCCCTCGCGATGACCATTTTCTTCCACGATATGACAATGCCTGAGCGTGTCTCGCTGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTg                                                                >  1:344859/1‑251 (MQ=255)                                                                                                                                                                                  tttCCTCGGGAGGTTACCTTGTTTCTCGCCAAAGAAATTATTCGTAAAAAACGTGAGTGGT‑ATGCACTGAGCGATGAAGAAATTCGTTTCTTTATCAACGGCATGCGAGACAACACTATCTCCGAAGTGCAGATTGCCGCCCTTGCGATGACCATGTTCTTCCACGATATGACAATGCCTGAGCGTGTCTCGCTGACCATGGCGATGCGGGATTTAGGAACCGTTCTCGACTGTAAAAGCCTGCATCTGa                                                               <  2:296078/250‑1 (MQ=255)                                                                                                                                                                                                      gTTTCTCGCACAAGAAATTATTCGTAAAAAACGTGA‑TGGTCATGCACTGAGCGATGAAGAAATTCGTTTCTTTATCAACGGCATTCGCGACAACACTATCTCCGAAGGGCAGATTGCCGCCCTCGCGATGACCATTTTCTTCCACGATATGACAATGCCTGAGCGTGTCTCGCTGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATaaa                                           <  1:146188/250‑1 (MQ=255)                                                                                                                                                                                                                   gAAATTATTCGTAAAAAACGTGA‑TGGTCATGCACTGAGCGATGAAGAAATTCGTTTCTTTATCAACGGCATTCGCGACAACACTATCTCCGAAGGGCAGATTGCCGCCCTCGCGATGACCATTTTCTTCCACGATATGACAATGCCTGAGCGTGTCTCGCTGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACtggtgg                             <  1:311109/251‑1 (MQ=255)                                                                                                                                                                                                                           tCGTAAAAAACGTGA‑TGGTCATGCACTGAGCGATGAAGAAATTCGTTTCTTTATCAACGGCATTCGCGACAACACTATCTCCGAAGGGCAGATTGCCGCCCTCGCGATGACCATTTTCTTCCACGATATGACAATGCCTGAGCGTGTCTCGCTGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCg                     >  1:409343/1‑251 (MQ=255)                                                                                                                                                                                                                               aaaaaaCGTGA‑TGGTCATGCACTGAGCGATGAAGAAATTCGTTTCTTTATCAACGGCATTCGCGACAACACTATCTCCGAAGGGCAGATTGCCGCCCTCGCGATGACCATTTTCTTCCACGATATGACAATGCCTGAGCGTGTCTCGCTGACCATGGCGATGCGAGACTCAGGAACCGTTCTCGCCTGGAAAAGCCTGCATCTGAATGGCCCGCTTGTTGATAAACATTCCACTGGTGGCGTGGGCGAtgg                 >  1:379735/1‑250 (MQ=255)                                                                                                                                                                                                                                 aaaaCGTGA‑TGGTCATGCACTGAGCGATGAAGAAATTCGTTTCTTTATCAACGGCATTCGCGACAACACTATCTCCGAAGGGCAGATTGCCGCCCTCGCGATGACCATTTTCTTCCACGATATGACAATGCCTGAGCGTGTCTCGCTGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGAtgtg                >  1:290734/1‑250 (MQ=255)                                                                                                                                                                                                                                               cATGCACTGAGCGATGAAGAAATTCGTTTCTTTATCAACGGCATTCGCGACAACACTATCTCCGAAGGGCAGATTGCCGCCCTCGCGATGACCATTTTCTTCCACGATATGACAATGCCTGAGCGTGTCTCGCTGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGtt  >  1:193234/1‑251 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                      TCCAGCCTGCTGGCAAGCCTGCTGAAAGCGCTGGGTCACGGCGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATCAGGCCTACGAGACGTATCTACCCGTAGGCCGGATAAGGCGTAGACGCATCCGGCAAAAGCCGCCTCATACTCTTTTCCTCGGGAGGTTACCTTGTTTCTCGCACAAGAAATTATTCGTAAAAAACGTGA‑TGGTCATGCGCTGAGCGATGAAGAAATTCGTTTCTTTATCAACGGTATTCGCGACAACACTATCTCCGAAGGGCAGATTGCCGCCCTCGCGATGACCATTTTCTTCCACGATATGACAATGCCTGAGCGTGTCTCGCTGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACACTCCACCGGTGGCGTCGGCGATGTGACTTCGCTGATGTT  >  NC_000913/4618034‑4618521

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑