Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,617,053 G→C H5D (CAT→GAT)  yjjI ← DUF3029 domain‑containing protein YjjI

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,617,0530GC97.7% 142.2 / ‑5.7 44H5D (CAT→GAT) yjjIDUF3029 domain‑containing protein YjjI
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base C (20/23);  total (21/23)
Fisher's exact test for biased strand distribution p-value = 4.77e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

CATTCGGAACCGTTCGCCAGAAAACGGGCGTAATCGGGTAAGACATAGCGCGGTTTGTACGGCGCATGACCTTCAAACATATCGCAGATTACACCTTCATCCAGCGCGCGGCGGGCTTCGGCAGGAAGCTGTGGGTAAGGCAGATTGTTTTCTGCTTCCAGTGCCAGAAAATGGCGCTTCTGCTCCGGGCTAAGCACTGGGCTGGTGACAATTTGCTGGCAACGTTGTTGCAGTGCATTTTCATGAGAAGTGGGCATCTTCTTTTCCTTTTATGCCGAAGGTGATGCGCCATTGTAAGAAGTTTCGTGATGTTCACTTTGATCCTGATGCGTTTGCCACCACTGACGCATTCATTTGAAAGTGAATTATTTGAACCAGATCGCATTACAGTGATGCAAACTTGTAAGTAGATTTCCTTAATTGTGATGTGTATCGAAGTGTGTTGCGGAGTAGATGTTAGAATACTAACAAACTCGCAAGGTGAATTT  >  NC_000913/4616809‑4617296
                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                   
cATTCGGAACCGTTCGCCAGAAAACGGGCGTAATCGGGTAAGACATAGCGCGGTTTGTACGGCGCATGACCTTCAAACATATCGCAGATTACAACTTCATCCAGCGCGCGGCGGGCTTCGGCAGGAAGCTGTGGGTCAGGCAGCTTGTTTTCTGCTTCCAGTGCAAGAAAATGGCGCTTCTGCTCCGGGCTAAGCACTGGGCTGGTGACAATTTGCTGGCAACGTTGTTGCAGTGCATTTTCATCAGAAGt                                                                                                                                                                                                                                               >  1:280939/1‑251 (MQ=255)
         ccGTTCGCCAGAAAACGGGCGTAATCGGGTAAGACATAGCGCGGTTTGTACGGCGCATGACCTTCAAACATATCGCAGATTACACCTTCATCCAGCGCGCGGCGGGCTTCGGCAGGAAGCTGTGGGTAAGGCAGATTGTTTTCTGCTTCCAGTGCCAGAAAATGGCGCTTCTGCTCCGGGCTAAGCACTGGGCTGGTGACAATTTGCTGGCAACGTTGTTGCAGTGCATTTTCATCAGAAGTGGGCAtctt                                                                                                                                                                                                                                      <  1:252032/251‑1 (MQ=255)
           gTTCGCCAGAAAACGGGCGTAATCGGGTAAGACATAGCGCGGTTTGTACGGCGCATGACCTTCAAACATATCGCAGATTACACCTTCATCCAGCGCGCGGCGGGCTTCGGCAGGAAGCTGTGGGTAAGGCAGATTGTTTTCTGCTTCCAGTGCCAGAAAATGGCGCTTCTGCTCCGGGCTAAGCACTGGGCTGGTGACAATTTGCTGGCAACGTTGTTGCAGTGCATTTTCATCAGAAGTGGGCAtcttct                                                                                                                                                                                                                                    >  1:69239/1‑251 (MQ=255)
             tCGCCAGAAAACGGGCGTAATCGGGTAAGACATAGCGCGGTTTGTACGGCGCATGACCTTCAAACATATCGCAGATTACACCTTCATCCAGCGCGCGTCGGGCTTCGGCATGAAGCTGTGGGTAAGGCATATTGTTTTCTGCTTCCAGTGCCAGAAAATGGCGCTTCTGCTCCGGGCTAAGCACTGGGCTGGTGACAATTTGCTGGCAACGTTGTTGCAGTGCATTTTCATCAGAAGTGGGCATcttctt                                                                                                                                                                                                                                   <  1:175280/250‑1 (MQ=255)
                ccAGAAAACGGGCGTAATCGGGTAAGACATAGCGCGGTTTGTACGGCGCATGACCTTCAAACATATCGCAGATTACACCTTCATCCAGCGCGCGGCGGGCTTCGGCAGGAAGCTGTGGGTAAGGCAGATTGTTTTCTGCTTCCAGTGCCAGAAAATGGCGCTTCTGCTCCGGGCTAAGCACTGGGCTGGTGCCAATTTGCTGGCCACGTTGTTGCAGTGCATTTTCATCAGAAGTGGGCATCTTCTTTTcc                                                                                                                                                                                                                               >  2:214695/1‑251 (MQ=255)
                              tAATCGGGTAAGACATAGCGCGGTTTGTACGGCGCATGACCTTCAAACATATCGCAGATTAAACCTTCATCCAGCGCGCGGCGGGCTTCGGCAGGAAGCTGTGGGTAAGGCAGATTGTTTTCTGCTTCCAGTGCCAGAAAATGGCGCTTCTGCTTCGGTCTAAGCACTGGGCTGATGACAATTTGCTGGCAACGTTGTTGCAGTGCATTTTCATCAGGAGTGGGCATCTTCTTTTCCTTTTATACCGAAgg                                                                                                                                                                                                                 >  2:20784/1‑251 (MQ=255)
                                  cGGGTAAGACATTGCGCGGTTTGTACGGCGCATGACCTTCAAACATATCGCAGATTACACCTTCATCCAGCGCGCGGCGGGCTTCGGCAGGAAGCTGTGGGTAAGGCAGATTGTTTTCTGCTTCCAGTGCCAGAAAATGGCGCTTCTGCTCCGGGCTAAGCACTGGGCTGGTGACAATTTGCTGGCAACGTTGTTGCAGTGCATGTTCATCAGAAGTGGGCATCTTCTTTTCCTTTTATGCCGAAGGTGAt                                                                                                                                                                                                             <  2:382450/251‑1 (MQ=255)
                                   gggTAAGACATAGCGCGGTTTGTACGGCGCATGACCTTCAAACATATCGCAGATTACACCTTCATCCAGCGCGCGGCGGGCTTCGGCAGGAAGCTGTGGGTAAGGCAGATTGTTTTCTGCTTCCAGTGCCAGAAAATGGCGCTTCTGCTCCGGGCTAAGCACTGGGCTGGTGACAATTTGCTGGCAACGTTGTTGCAGTGCATTTTCATCAGAAGTGGGCATCTTCTTTTCCTTTTATGCCGAAGGTGATg                                                                                                                                                                                                            >  1:238873/1‑251 (MQ=255)
                                   gggTAAGACATAGCGCGGTTTGTACGGCGCATGACCTTCAAACATATCGCAGATTACACCTTCATCCAGCGCGCGGCGGGCTTCGGCAGGAAGCTGTGGGTAAGGCAGATTGTTTTCTGCTTCCAGTGCCAGAAAATGGCGCTTCTGCTCCGGACTAAGCACTGGGCTGGTGACAATTTGCTGGCAACGTTGTTGCAGTGCATTTTCATCAGAAGTGGGCATCTTCTTTTCCTTTTATGCCGAAGGTGAtt                                                                                                                                                                                                            >  1:291173/1‑250 (MQ=255)
                                     gTAAGACATAGCGCGGTGTGTTCGGTGTATGACCTTCAAACAAAGCTCGGATTACACTTTCATCCAGCGCGCGTCGGGCTTCGGCAGGAAGCTGTGGGTAAGGCAGATTGTTTTCTGCTTCCAGTGCCAGAAAATGGCGCTTCTGCGCCGGGCTAAGTACTGGGCTGGTGACTATTTGCTGGCAACGTTGTGGCAGTGTATTTTCATCAGAAGTTGGTATCTGCTTTTCCTTTTATGCCGATTGTGATgcg                                                                                                                                                                                                          <  2:280715/251‑1 (MQ=255)
                                                 gcgGTTTGTACGGCGCATGACCTTCAAACATATCGCAGATTACACCTTCATCCAGCGCGCGGCGGGCTTCGGCAGGAAGCTGTGGGTAAGGCAGATTGTTTTCTGCTTCCAGTGCCAGAAAATGGCGCTTCTGCTCCGGGCTAAGCACTGGGCTGGTGACAATTTGCTGGCAACGTTGTTGCAGTGCATTTTCATCAGAAGTGGGCATCTTCTTTTCCTTTTATGCCGAAGGTGATGCGCCATTGTaagaa                                                                                                                                                                                              <  1:108386/251‑1 (MQ=255)
                                                       tGTACGGCGCATGACCTTCAAACATATCGCAGATTACACCTTCATCCAGCGCGCGGCGGGCTTCGGCAGGAAGCTGTGGGTAAGGCAGATTGTTTTCTGCTTCCCGTGCCAGGAAATGGCGCTTCTGCTCCGGGCTAAGCACTGGGCTGGTGACAATTTGCTGGCAAAGTTGTTGCAGTGCATTTTCATCCGAAGTGGGCATCTTCTTTTCCTTTTATGCCGAAGGTGCTGCGCCATTGTAAGGAGTTTCg                                                                                                                                                                                        >  2:396346/1‑251 (MQ=255)
                                                       tGTACGGCGCACGACCTTCAAACATAGCGCAGATTACACCTTCATCCAGCGCGCGGCGGGCTTCGGCAGGAAGCTGTGGGTAAGGCAGATTGTTTTCTGCTTCCAGTGCCAGAAAATGGCGCTTCTGCTCCGGGCTAAGCACTGGGCTGGTGACAATTTGCTGGCAACGTTGTTGCAGTGCATTTTCATCAGAAGTGGGCATCTTCTTTTCCTTTTATGCCGAAGGTGATGCGCCATTGTAAGAAGTTTc                                                                                                                                                                                         <  2:369949/250‑1 (MQ=255)
                                                              cgcATGACCTTCAAACATATCGCAGATTACACCTGCATCCAGCGCGCGGCGGGCTTCGGCAGGAAGCTGTGGGTAAGGCAGATTGTTTTCTGCTTCCAGTGCCAGAAAATGGCGCTTCTGCTCCGGGCTAAGCACTGGGCTGGTGACAATTTGCTGGCAACGTTGTTGCAGTGCATTTTCATCAGAAGTGGGCATCTTCTTTTCCTTTTATGCCGAAGGTGATGCGCCATTGTAAGAAGTTTCGTGATGtt                                                                                                                                                                                 <  2:436665/251‑1 (MQ=255)
                                                                                   gCAGATTACACCTTCATCCAGCGCGCGGCGGGCTTCGGCAGGAAGCTGTGGGTAAGGCAGATTGTTTTCTGCTTCCAGTGCCAGAAAATGGCGCTGCTGCTCCGGGCTAAGCACTGGGCTGGTGACAATTTGCTGGCAACGTTGTTGCAGTGCATTTTCATCAGAAGTGGGCATCTTCTTTTCCTTTTATGCCGAAGGTGATGCGCCATTGTAAGAAGTTTCGTGATGTTCACTTTGATCCTGATGAGttt                                                                                                                                                            <  2:69239/251‑1 (MQ=255)
                                                                                     aGATTACACCTTCATCCAGCGCGCGGCGGGCTTCGGCAGGAAGCTGTGGGTAAGGCAGATTGTTTTCTGCTTCCAGTGCCAGAAAATGGCGCTTCTGCTCCGGGCTAAGCACTGGGCTGGTGACAATTTGCTGGCAACGTTGTTGCAGTGCATTTTCATCAGAAGTGGGCATCTTCTTTTCCTTTTATGCCGAAGGTGATGCGCCATTGTAAGAAGTTTCGTGATGTTCACTTTGATCCTGATGAGTTTGc                                                                                                                                                          <  2:143847/251‑1 (MQ=255)
                                                                                      gATTACACCTTCATCCAGCGCGCGGCGGGCTTCGGCAGGAAGCTGTGGGTAAGGCAGATTGTTTTCTGCTTCCAGTGCCAGAAAATGGCGCTTCTGCTCCGGGCTAAGCACTGGGCTGGTGACAATTTGCTGGCAACGTTGTTGCAGTGCATTTTCATGAGAAGTGGGCATCTTCTTTTCCTTTTATGCCGAAGGTGATGCGCCATTGTAAGAAGTTTCGTGATGTTCACTTTGATCCTGATGCGTTTGcc                                                                                                                                                         >  2:394738/1‑251 (MQ=255)
                                                                                                    ccAGCGCGCGGCGGGCTTCGGCAGGAAGCTGTGGGTAAGGCAGATTGTTTTCTGCTTCCAGTGCCAGAAAATGGCGCTTCTGCTCCGGGCTAAGCACTGGGCTGGTGACAATTTGCTGGCAACGTTGTTGCAGTGCATTTTCATCAGAAGTGGGCATCTTCTTTTCCTTTTATGCCGAAGGTGATGCGCCATTGTAAGAAGTTTCGTGATGTTCACTTTGATCCTGATGAGTTTGCCACCACTGACGcat                                                                                                                                            <  2:388060/250‑1 (MQ=255)
                                                                                                      aGCGCGCGGCGGGCTTCGGCAGGAAGCTGTGGGTAAGGCAGATTGTTTTCTGCTTCCAGTGCCAGAAAATGGCGCTTCTGCTCCGGGCTAAGCACTGGGCTGGTGACAATTTGCTGGCAACGTTGTTGCAGTGCATTTTCATCAGAAGTGGGCATCTTCTTTTCCTTTTATGCCg                                                                                                                                                                                                                     >  1:219719/1‑175 (MQ=255)
                                                                                                      aGCGCGCGGCGGGCTTCGGCAGGAAGCTGTGGGTAAGGCAGATTGTTTTCTGCTTCCAGTGCCAGAAAATGGCGCTTCTGCTCCGGGCTAAGCACTGGGCTGGTGACAATTTGCTGGCAACGTTGTTGCAGTGCATTTTCATCAGAAGTGGGCATCTTCTTTTCCTTTTATGCCg                                                                                                                                                                                                                     <  2:219719/175‑1 (MQ=255)
                                                                                                      aGCGCGCGGCGGGCTTCGGCAGGAAGCTGTGGGTAAGGCAGATTGTTTTCTGCTTCCAGTGCCAGAAAATGGCGCTTCTGCTCCGGGCTAAGCACTGGGCTGGTGACAATTTGCTGGCAACGTTGTTGCAGTGCATTTTCATCAGAAGTGGGCATCTTCTTTTCCTTTTATGCCGAAGGTGATGCGCCATTGTAAGAAGTTTCGTGATGTTCACTTTGATCCTGATGAGTTTGCCACCACTGACGcattca                                                                                                                                         <  2:238873/251‑1 (MQ=255)
                                                                                                                      cGGCAGGAAGCTGTGGGTAAGGCAGATTGTTTTCTGCTTCCAGTGCCAGAAAATGGCGCTTCTGCTCCGGGCTAAGCACTGGGCTGGTGACAATTTGCTGGCAACGTTGTTGCAGTGCATTTTCATCAGAAGTGGGCATCTTCTTTTCCTTTTATGCCGAAGGTGATGCGCCATTGTAAGAAGTTTCGTGATGTTCACTTTGATCCTGATGAGTTTGCCACCACTGACGCATTCATTTGAAAGTGAattat                                                                                                                         <  1:125969/251‑1 (MQ=255)
                                                                                                                                  gtgGGTAAGGCAGATTGTTTTCTGCTTCCAGTGCCAGAAAATGGCGCTTCTGCTCCGGGCTAAGCACTGGGCTGGTGACAATTTGCTGGCAACGTTGTTGCAGTGCATTTTCATCAGAAGTGGGCATCTTCTTTTCCTTTTATGCCGAAGGTGATGCGCCATTGTAAGAAGTTTCGTGATGTTCACTTTGATCCTGATGAGTTTGCCACCACTGACGCATTCATTTGAAAGTGAATTATTTGAACCAGa                                                                                                               <  1:243281/249‑1 (MQ=255)
                                                                                                                                  gtgGGTAAGGCAGATTGTTTTCTGCTTCCAGTGCCAGAAAATGGCGCTTCTGCTCCGGGCTAAGCACTGGGCTGGCGACAATTTGCTGGCACCGTTGTTGCAGTGCATTTTCATCAGAAGTGGGCATCTTCTTTTCCTTTTATGCAGAAGGTGATGCGCCATTGTAAGAAGTTTCGTGATGTTCACTTTGATCCTGATGAGTTTGCCACCACTGACGCATTCATTTGAAAGTGAATTATTTGAAcc                                                                                                                  >  1:402842/1‑246 (MQ=255)
                                                                                                                                      gTAAGGCAGATTGTTTTCTGCGTCCAGTGCCAGAAAATGGCGCTTCTGCTCCGGGCTAAGCACTGGGCTGGAGACAATTTGCTGGCAACGTTGTTGCAGTGCATTTTCATCAGAAGTGGGCATCTTCTTTTCCTTTTATGCCGAAGGTGATGCGCCATTGTAAGAAGTTTCGTGATGTTCACTTTGATCCTGATGAGTTTGCCACCAATGACGCATTCATTTGAAAGTGATTTATTTAAACCAGATAGCAt                                                                                                         >  2:476351/1‑251 (MQ=255)
                                                                                                                                            cAGATTGTTTTCTGCTTCCAGTGCCAGAAAATGGCGCTTCTGCTCCGGGCTAAGCACTGGGCTGGTGACAATTTGCTGGCAACGTTGTTGCAGTGCATTTTCATCAGAAGTGGGCATCTTCTTTTCCTTTTATGCCGAAGGTGATGCGCCATTGTAAGAAGTTTCGTGATGTTCACTTTGATCCTGATGAGTTTGCCACCACTGACGCATTCATTTGAAAGTGAATTATTTGAACCAGATCGCATTACAGt                                                                                                   <  1:184554/251‑1 (MQ=255)
                                                                                                                                                      tCTGCTTCCAGTGCCAGAAAATGGCGCTTCTGCTCCGGGCTAAGCACTGGGCTGGTGACAATTTGCTGGCAACGTTGTTGCAGTGCATTTTCATCAGAAGTGGGCATCTTCTTTTCCTTTTATGCCGAAGGTGATGCGCCATTGTAAGAAGTTTCGTGATGTTCACTTTGATCCTGATGAGTTTGCCACCACTGACGCATTCATTTGAAAGTGAATTATTTGAACCAGATCGCATTACAGTGATGCAAACt                                                                                         >  2:228967/1‑251 (MQ=255)
                                                                                                                                                           ttCCAGTGCCAGAAAATGGCGCTTCTGCTCCGGGCTAAGCACTGGGCTGGTGACAATTTGCTGGCAACGTTGTTGCAGTGCATTTTCATCAGAAGTGGGCATCTTCTTTTCCTTTTAt                                                                                                                                                                                                                         >  1:127780/1‑118 (MQ=255)
                                                                                                                                                           ttCCAGTGCCAGAAAATGGCGCTTCTGCTCCGGGCTAAGCACTGGGCTGGTGACAATTTGCTGGCAACGTTGTTGCAGTGCATTTTCATCAGAAGTGGGCATCTTCTTTTCCTTTTAt                                                                                                                                                                                                                         <  2:127780/118‑1 (MQ=255)
                                                                                                                                                              cagggCCAGAAAATGGCGCTTCTGCTCCGGGCTAAGCACTGGGCTGGTGACAATTTTCTGGCAACGTTGTTGCAGTGCATTTTCATCAGAAGTGGGCAGCTTCTTTTCCTTTTATGCCGAAGGTGATGCGCCATTGTAAGAAGTTTCGTGATGTTCACTTTGATCCGGATGAGTTTGCCACCACTGACGCATTCATTTGAAAGTGAATTATTTGAACCAGATCGCATTACAGTGATGCAAACTTGTAAGTa                                                                                 <  2:244380/247‑1 (MQ=255)
                                                                                                                                                                    cAGAAAATGGCGCTTCTGCTCCGGGCTAAGCACTGGGCTGGTGACAATTTGCTGGCAACGTTGTTGCAGTGCATTTTCATCAGAAGTGGGCATCTTCTTTTCCTTTTATGCCGAAGGTGATGCGCCATTGTAAGAAGTTTCGTGATGTTCACTTTGATCCTGATGAGTTTGCCACCACTGACGCATTCATTTGAAAGTGAATTATTTGAACCAGATCGCATTACAGTGATGCAAACTTGTAAGTAGATTTc                                                                           >  2:459037/1‑251 (MQ=255)
                                                                                                                                                                            ggCGCTTCTGCTCCGGGCTAAGCACTGGGCTGGTGACAATTTGCTGGCAACGTTGTTGCAGTGCATTTTCATCAGAAGTGGGCATCTTCTTTTCCTTTTATGCCGAAGGTGATGCGCCATTGTAAGAAGTTTCGTGATGTTCACTTTGATCCTGATGAGTTTGCCACCACTGACGCATTCATTTGAAAGTGAATTATTTGAACCAGATCGCATTACAGTGATGCAAACTTGTAAGTAGATTTCCTTAAtt                                                                    <  1:424572/250‑1 (MQ=255)
                                                                                                                                                                                                 gCACTGGGCTGGTGACAATTTGCTGGCAACGTTGTTGCAGTGCATTTTCATCAGAAGTGGGCATCTTCTTTTCCTTTTATGCCGAAGGTGATGCGCCATTGTAAGAAGTTTCGTGATGTTCACTTTGATCCTTATGAGTTTGCCACCACTGACGCATTCATTTGAAAGTGAATTATTTGAACCAGATCGCATTACATTGATGCAAACTTGTAAGTAGATTTCCTTAATTGTGATGTATATCGAAGGGTGtt                                              <  1:396330/251‑1 (MQ=255)
                                                                                                                                                                                                      gggCTGGTGACAATTTGCTGGCAACGTTGTTGCAGTGCATTTTCATCAGAAGTGGGCATCTTCTTTTCCTTTTATGCCGAAGGTGATGCGCCATTGTAAGAAGTTTCGTGATGTTCACTTTGATCCTGATGAGTTTGCCACCACTGACGCATTCATTTGAAAGTGAATTATTTGAACCAGATCGCATTACAGTGATGCAAACTTGTAAGTAGATTTCCTTAATTGTGATGTATATCGAAGTGTGTTGCggg                                         >  2:390947/1‑250 (MQ=255)
                                                                                                                                                                                                              aaCAATTTGCGGGCAAAGTTGTTGCAGTGCATTTTCATCAGAAGAGGGCATCTTCTTTTCCTTTTATGCCGAAGGTGATGCGCAATAGTAAGAAGTTTCTTGATGTTCACTTTGATCCTGATGAGTATGCCACCACTGACGCATTCATTTGAAATTGAATTATTTGAACCAGATCGCATTACCGTGAGGCAATCTTGTAATTATATTTCCTTAATTTCGATGTATATCGAAgggagttggtgggtatatgt                                 >  2:10781/2‑232 (MQ=255)
                                                                                                                                                                                                                 aaTTTGCTGGCAACGTTGTTGCAGTGCATTTTCATCAGAAGTGGGCATCTTCTTTTCCTTTTATGCCGAAGGTGATGCGCCATTGTAAGAAGTTTCGTGATGTTCACTTTGATCCTGATGAGTTTGCCACCACTGACGCATTCATTTGAAAGTGAATTATTTGAACCAGATCGCATTACAGTGATGCAAACTTGTAAGTAGATTTCCTTAATTGTGATGTATATCGAAGTGTGTTGCGGAGTAGATGtt                                >  1:160787/1‑249 (MQ=255)
                                                                                                                                                                                                                         ggtaaCGGTGTTGCAGGGCATTTTCATCAGAAGTGGGCATCTTCTTTGCCTTTTAGGCCGAAGGTGATGCGCCATTGTAAGAAGTTTCGTGATGTTCACTTTGATCCTGATGAGTTTTCCACCACTGACGCATTCATTTGAAAGTGAATTATTTGAACCAGATCGCATTACAGTGATGCAAACTTGTAAGTAGATTTCCTTAATTGTGATGTATATCGAAGTGTGTTGCGGAGTAGATGTTAGAATACTaa                      <  2:402842/248‑1 (MQ=255)
                                                                                                                                                                                                                           cAACGTTGTTGCAGTGCATTTTCATCAGAAGTGGGCATCTTCTTTTCCTTTTATGCCGAAGGTGATGCGCCATTGTAAGAAGTTTCGTGATGTTCACTTTGATCCTGATGAGTTTGCCACCACTGACGCATTCATTTGAAAGTGAATTATTTGAACCAGATCGCATTACAGTGATGCAAACTTGTAAGTAGATTTCCTTAATTGTGATGTATATCGAAGTGTGTTGCGGAGTAGATGTTAGAATACTAAc                     >  1:199674/1‑250 (MQ=255)
                                                                                                                                                                                                                              cGTTGTTGCAGTGCATTTTCATCAGAAGTGGGCATCTTCTTTTCCTTTTATGCCGAAGGTGATGCGCCATTGTAAGAAGTTTCGTGATGTTCACTTTGATCCTGATGAGTTTGCCACCACTGACGCATTCATTTGAAAGTGAATTATTTGAACCAGATCGCATTACAGTGATg                                                                                               <  1:431924/173‑1 (MQ=255)
                                                                                                                                                                                                                              cGTTGTTGCAGTGCATTTTCATCAGAAGTGGGCATCTTCTTTTCCTTTTATGCCGAAGGTGATGCGCCATTGTAAGAAGTTTCGTGATGTTCACTTTGATCCTGATGAGTTTGCCACCACTGACGCATTCATTTGAAAGTGAATTATTTGAACCAGATCGCATTACAGTGATg                                                                                               >  2:431924/1‑173 (MQ=255)
                                                                                                                                                                                                                                 ggttgCAGTGCATTTTCATCAGAAGTGGGTATCTTCTTTTGCTTTTATGCCGAAGGTGATGCGCCATTGTAAGAAGTTTCGTGATGTTCACTTTGATCCTGTTTAGTATGTCATCACTGACGAATTCATTTGAAAGTGAAGGATTTTAGCCAGATCGCATTACAGTGATGCAAACTGGTAAGTAGATTTTCTTAATTATGACGTATATCGAAGCGTGTTGCGGAGTAGATGTTAGAATACTAACAAACTCg              <  2:124378/250‑1 (MQ=255)
                                                                                                                                                                                                                                  gttgCAGTGCATTTTCATCAGAAGTGGGCATCTTCTTTTCCTTTTATGCCGAAGGTGATGCGCCATTGTAAGAAGTTTCGTGATGTTCACTTTGATCCTGATGAGTTTGCCACCACTGACGCATTCATTTGAAAGTGAATTATTTGAACCAGATCGCATTACAGTGATGCAAACTTGTAAGTAGTTTTCCTTAATTGTGATGTATATCGAAGTGTGTTGCGGAGTAGATGTTAGAATACTAACAAACTCGc             >  2:463292/1‑251 (MQ=255)
                                                                                                                                                                                                                                            aTTTTAATCAGAAGTGGGCATCTTCTTTTCCTTTTATGCCGAAGTTGATGCGCCATTGTAAGAAGTTTCGTGATGTTCACTTTGATCCTGATGAGTTTGCCACCACTGACGCATTCATTTGAAAGTGAATTATTTGAACCAGATCGCATTACAGTGATGCAACCTTGTAAGTAGATTTCCTTAATTGTGATGTATATCGAAGTGAGTTTCGGAGTTGATGTTAGAATACTACCAAACTCGCAAGGTGAAtt   >  2:16768/1‑251 (MQ=255)
                                                                                                                                                                                                                                              tttCATCAGAAGTGGGCATCTTCTTTTCCTTTTATGCCGAAGGTGATGCGCCATTGTAAGAAGTTTCGTGATGTTCACTTTGATCCTGATGAGTTTGCCACCACTGACGCATTCATTTGAAAGTTAATTATTTGAACCAGATCGCATTACAGTGATGCAAACTTGTAAGTAGATTTCCTTAATTGTGATGTATATCGAAGTGTGTTGCGGAGTAGATGTTAGAATACTAACAAACTCGCAAGGTGAAttt  <  2:149971/250‑1 (MQ=255)
                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                   
CATTCGGAACCGTTCGCCAGAAAACGGGCGTAATCGGGTAAGACATAGCGCGGTTTGTACGGCGCATGACCTTCAAACATATCGCAGATTACACCTTCATCCAGCGCGCGGCGGGCTTCGGCAGGAAGCTGTGGGTAAGGCAGATTGTTTTCTGCTTCCAGTGCCAGAAAATGGCGCTTCTGCTCCGGGCTAAGCACTGGGCTGGTGACAATTTGCTGGCAACGTTGTTGCAGTGCATTTTCATGAGAAGTGGGCATCTTCTTTTCCTTTTATGCCGAAGGTGATGCGCCATTGTAAGAAGTTTCGTGATGTTCACTTTGATCCTGATGCGTTTGCCACCACTGACGCATTCATTTGAAAGTGAATTATTTGAACCAGATCGCATTACAGTGATGCAAACTTGTAAGTAGATTTCCTTAATTGTGATGTGTATCGAAGTGTGTTGCGGAGTAGATGTTAGAATACTAACAAACTCGCAAGGTGAATTT  >  NC_000913/4616809‑4617296

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑