Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,614,170	C→T	F230F (TTC→TTT)	yjjV →	putative metal‑dependent hydrolase YjjV

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,614,170	0	C	T	100.0%	197.5 / NA	62	F230F (TTC→TTT)	yjjV	putative metal‑dependent hydrolase YjjV
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (31/31);  total (31/31)

TACCGCGCACTGGCGTGGTTCACGGTTTTTCCGGCAGCCTGCAACAGGCCGAACGGTTTGTACAGCTGGGCTACAAAATTGGCGTAGGCGGTACTATCACCTATCCACGCGCCAGTAAAACCCGCGATGTCATCGCAAAATTACCGCTGGCATCGTTATTGCTGGAAACCGACGCGCCGGATATG‑CCGCTCAACGGTTTTCAGGGGCAGCCTAACCGCCCGGAGCAGGCTGCCCGTGTGTTCGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGC  >  NC_000913/4613929‑4614414                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                     tACCGCGCACTGGCGTGGTTCACGGTTTTTCCGGCAGCCTGCAACAGGCCGAACGGTTTGTACAGCTGGGCTACAAAATTGGCGTAGGCGGTACTATCACCTATCCACGCGCCAGTAAAACCCGCGATGTCATCGCAAAATTACCGCTGGCATCGTTATTGCTGGAAACCGACGCGCCGGATATG‑CCGCTCAACGGTTTTCAGGGGCAGCCTAACCGCCCGGAGCCGGCTGCCCGTTTGTTTGCCGTGCtt                                                                                                                                                                                                                                             >  1:232938/1‑251 (MQ=255) tACCGCGCACTGGCGTGGTTCACGGTTTTTCCGGCAGCCTGCAACAGGCCGAACGGTTTGTACAGCTGGGCTACAAAATTGGCGTAGGCGGTACTATCACCTATCCACGCGCCAGTAAAACCCGCGATGTCATCGCAAAATTACCGCTGGCATCGTTATTGCTGGAAACCGACGCGCCGGATATG‑CCGCTCAACGGTTTTCAGGGGCAGCCTAACCGCCCGGAGCAGGCTGCCCGTGTGTTTGCCGTGCtt                                                                                                                                                                                                                                             >  1:476629/1‑251 (MQ=255)      cgcACTGGCGTGGTTCACGGTTTTTCCGGCAGCCGGCAACAGGCCGAACGGTTTGTACAGCGGGGCTACAAAATTGGCGTAGGCGGTACTATCACCTATCCACGCGCCAGTAAAACCCGCGATGTCATCGCAAAATTACCGCTGGCATCGTTATTGCTGGAAACCGACGCGCCGGATATG‑CCGCTCAACGGTTTTCAGGGGCAGCCTAACCGCCCGGAGCAGGCTGCCCGTGTGTTTGCCGTGCTTTGc                                                                                                                                                                                                                                          <  2:103632/249‑1 (MQ=255)         aCTGGCGTGGTTCACGGTTTTTCCGGCAGCCTGCAACAGGCCGAACGGTTTGTACAGCTGGGCTACAAAATTGGCGTAGGCGGTACTATCACCTATCCACGCGCCAGTAAAACCCGCGATGTCATCGCCAAATTACCGCTGGCATCGTTATTGCTGGAAACCGACGCGCCGGATATG‑CCGCTCAACGGGTTTCAGGGGCAGCCTAACCGCCCGGAGCAGGCTGCCCGTGTGTTTGCCGTGCTTTGCGAGtt                                                                                                                                                                                                                                     >  1:286521/1‑251 (MQ=255)                  gTTCACGGTTTTGCCGGCAGCCTGCAACAGGCCGAACGGTTTGTACAGCTGGGCAACAAAATTTGCGTAGGCGGTACTATCACCTATCCACGCGCCAGTAAAACCCGCGATGTCATCGCAAAATTACCGCTGGCATCGTTATTGCTGGAAACCGACGCGCCGGATATG‑CCGCTCAACGGTTTTCAGGGGCAGCCTAACCGCCCGGAGCAGGCTGCCCGTGTGTTTGCCGTGCTTTGCGAGTTGCGCCGGGa                                                                                                                                                                                                                            <  1:279427/251‑1 (MQ=255)                   ttCACGGTTTTTCCGGCAGCCTGCAACAAGCCTAACGGTTTTTCCCGCTGGGCTACAAAATTGTCGTCGGCGGTACTATCCACTATCCACGCGCCCGTAAAACCCGCGATGTCAGCGCAAAATTACCGCTGGCATCGTTATTGCTGGAAACCAACGCCCCGGATATG‑CCGCTCAACTGTTTTCAGGGGCAGCCTACCCGCCCGGAGCATGCTGCCCGTGTGTTTGCCTTTCGTTGCGAGCTGCGCCgggg                                                                                                                                                                                                                            >  2:434807/1‑249 (MQ=255)                    tCACGGTTTTTCCGGCAGCCTGCAACAGGCCGAACGGTTTGTACAGCTGGGCTACAAAATTGGCGTAGGCGGTACTATCACCTATCCACGCGCCAGTAAAACCCGCGATGTCATCGCAAAATTACCGCTGGCATCGTTATTGCTGGAAACCGACGCGCCGGATATG‑CCGCTCAACGGTTTTCAGGGGCAGCCTAACCGCCCGGAGCAGGCTGCCCGTGTGTTTGCCGTGCTTTGCGAGTTGCGCCGGGaa                                                                                                                                                                                                                           <  1:8034/250‑1 (MQ=255)                         gTTTTTCCGGCAGCCTGCAACAGGCCGAACGGTTTGTACAGCTGGGCTACAAAATTGGCGTAGGCGGTACTATCACCTATCCACGCGCCAGTAAAACCCGCGATGTCATCGCAAAATTACCGCTGGCATCGTTATTGCTGGAAACCGACGCGCCGGATATG‑CCGCTCAACGGTTTTCAGGGGCAGCCTAACCGCCCGGAGCAGGCTGCCCGTGTGTTTGCCGTGCTTTGCGAGTTGCGCCGGGAACcggcg                                                                                                                                                                                                                     <  1:14609/251‑1 (MQ=255)                         gTTTTTCCGGCAGCCTGCAACAGGCCGAACGGTTTGTACAGCTGGGCTACAAAATTGGCGTAGGCGGTACTATCACCTATCCACGCGCCAGTAAAACCCGCGATGTCATCGCAAAATTACCGCTGGCATCGTTATTGCTGGAAACCGACGCGCCGGATATG‑CCGCTCAACGGTTTTCAGGGGCAGCCTAACCGCCCGGAGCAGGCTGCCCGTGTGTTTGCCGTGCTTTGCGAGTTGCGCCGGGAACcggcg                                                                                                                                                                                                                     >  1:39937/1‑251 (MQ=255)                          tttttCCGGCAGCCTGCAACAGGCCGAACGGTTTGTACAGCTGGGATACAAAATTGGCGTAGGCGGTACTATCACCTATCCACGCGCCAGTAAAACCCGCGATGTCATCGCAAAATTACCGCTGGCATCGTTATTGCTGGAAACCGACGCGCCGGATATG‑CCGCTCAACGGTTTTCAGGGGCAGCCTAACCGCCCGGAGCAGGCTGCCCGTGTGTTTGCCGTGCTTTGCGAGTTGCGCCGGGAACcggcg                                                                                                                                                                                                                     <  1:101860/250‑1 (MQ=255)                          tgtttCCGGCAGCCTGCACCAGGCCGAACGGGTTGTACAGCTGGGCTACAAAATTGGCGTAGGCGGTACTATCACCTATCCACGCGCCAGTAAAACCCGCGATGTCATCGCAAAATTACCGCTGGCATCGTTATTGCTGGAAACCGACGCGCCGGATATG‑CCGCTCAACGGTTTTCAGGGGCAGCCTAACCGCCCGGAGCAGGCTGCCCGTGTGTTTGCCGTGCTTTGCGAGTTGCGCCGGGAACcggcg                                                                                                                                                                                                                     <  2:477135/248‑1 (MQ=255)                               ccGGCAGCCTGCAACAGCCCGAACGGTTTGTCCAGCTGGCATACAAAATTGGCGTAGGCGGTACTCTCACCTCTCCACGCGGCAGTAAAACCCGCGATGTCATCGCAAAATTACCGCTGGCATCGTTATTGCTGGAAACCGACGCGCCGGATATG‑CCGCTCAACGGTTTTCAGGGGCAGCCTAACCGCCCGGAGCAGGCTGCCTGTGTGTTTGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATga                                                                                                                                                                                                                <  1:141108/250‑1 (MQ=255)                                   cAGCCTGCAACAGGCCGAACGGTTTGTACAGCTGGGCTACAAAATTGGCGTAGGCGGTACTATCACCTATCCACGCGCCAGTAAAACCCGCGATGTCATCGCAAAATTACCGCTGGCATCGTTCTTGCTGGAAACCGACGCGCCGGATATG‑CCGCTCAACGGTTTTCAGGGGCAGCCTAACCGCCCGGCGAAGGCTGCCCGTGTGTTTGCCGTGCTTTGCGAGATGCGCCGGGACCcggcgggggaggttg                                                                                                                                                                                                           >  2:322220/1‑242 (MQ=255)                                    aGCCTGCAACAGGCCGAACGGTTTGTACAGCTGGGCTACAAAATTGGCGTAGGCGGTACTATCACCTATCCACGCGCCAGTAAAACCCGCGATGTCATCGCAAAATTACCGCTGGCATCGTTATTGCTGGAAACCGGCGCGCCGGATATG‑CCGCTCAACGGTTTTCAGGGGCAGCCTAACCGCCCGGAGCAGGCTGCCCGTGTGTTTGCCGTGCTTTGAGGGGTGAGCCGGGGACcggcgggggaggttgg                                                                                                                                                                                                          >  2:280299/1‑241 (MQ=255)                                       cggCAACAGGCCGAACGGGTTGTACAGCTGGGTTACAAAATTGGCGTAGGCGGTACTATCACCTATCCACGCGCCAGTAAAACCCGCGATGTCATCGCAAAATTACCGCTGGCATCGTTATTGCTGGAAACCGACGCGCCGGATATG‑CCGCTCAACGGTTTTCAGGGGCAGCCTAACCGCCCGGAGCAGGCTGCCCGTGTGTTTGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCa                                                                                                                                                                                                       <  2:201293/249‑1 (MQ=255)                                             cAGGCCGAACGGTTTGTACAGCTGGGCTACAAAATTGGCGTAGGCGGTACTATCACCTATCCACGCGCCAGTAAAACCCGCGATGTCATCGCAAAATTACCGCTGGCATCGTTATTGCTGGAAACCGACGCGCCGGATATG‑CCGCTCAACGGTTTTCAGGGGCAGCCTAACCGCCCGGAGCAGGCTGCCCGTGTGTTTGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGtt                                                                                                                                                                                                 <  1:286797/251‑1 (MQ=255)                                                     aCGGTTTGTACAGCTGGGCTACAAAATTGGCGTAGGCGGTACTATCACCTATCCACGCGCCAGTAAAACCCGCGATGTCATCGCAAAATTACCGCTGGCATCGTTATTGCTGGAAACCGACGCGCCGGATATG‑CCGCTCAACGGTTTTCAGGGGCAGCCTAACCGCCCGGAGCAGGCTGCCCGTGTGTTTGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTtaata                                                                                                                                                                                         <  2:459728/251‑1 (MQ=255)                                                      ggtgtggaaCAGATGGGCTACAAAATTGGCGGAGGCGGTACTATCACCTACCCACGCGCCAGTAAAACCCGCGAGGGCATCGCAAAATTACCGCTGGCATCGTTATCGCTGGAAACCGACGCGCCGGATATG‑CCGCGCAACGGTTTTCAGGGGCAGCCTATCCGCCCGGAGCAGGCTGCCTGTGTGTTTGCCGTGCTTTGCGAGTTGCGCCGGGAACCAGCGGATGAGATTGCGCAAGCGTTGCTtaataa                                                                                                                                                                                        <  2:229322/243‑1 (MQ=255)                                                        gTTTGTACAGCTGGGCTAAAAAATTGGCGTAGGCGGTACTATCTCCTAGCCACGCGCCAGTAAAGCCCGCGATGCCATCGAAAAATTACCGCTGGCATCGTTATTGCTGGAAACCGACTCGCCGGATATG‑CCGCTCAACGGTTTTCAGGGGCAGCCTAACCGCCCGGAGCAGGCTGCCCGTGTGTTTGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAaca                                                                                                                                                                                      <  2:3302/251‑1 (MQ=255)                                                                    gggCTACAAAATTGGCGTAGGCGGTACTATCACCTATCCACGCGCCAGTAAAACCCGCGATGTCATCGCAAAATTACCGCTGGCATCGTTATTGCTGGAAACCGACGCGCCGGATATG‑CCGCTCAACGGTTTTCAGGGGCAGCCTAACCGCCCGGAGCAGGCTGCCCGTGTGTTTGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACgttg                                                                                                                                                                           <  1:395973/250‑1 (MQ=255)                                                                           aaaaTTGGCGTAGGCGGTACTATCACCTATCCACGCGCCAGTAAAACCCGCGATGTCATCGCAAAATTACCGCTGGCATCGTTATTGCTGGAAACCGACGCGCCGGGTATG‑CCGCTCAACGGTTTTCAGGGGCAGCCTAACCGCCCGGTGCAGGCTGCCCGTGTGTTTGCCGTGCTTTGCGGGTTGCGCCGGGAACCGGCGGATGGGGTTGCGGACGCGGTGGTTAATAACACggattcctgttttacagt                                                                                                                                                                   >  2:387669/1‑234 (MQ=255)                                                                                  gggTAGGCGGTACTATCACCTAGCCACGCGCCAGTAAAACCCGCGATGTCATCGCAAAATTACCGCTGGCATCGTTATTGCTGGAAACCGACGCGCCGGATATG‑CCGCTCAACGGTTTTCAGGGGCAGCCTAACCGCCCGGAGCAGGCTGCCCGTGTGTTTGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAg                                                                                                                                                            <  2:147410/249‑1 (MQ=255)                                                                                  gCGTAGGCGGTACTATCCCCTAGCCACGCGCCAGGAAAACCCGCGATGTCATCGAAAAATTACCGCTGGCATCGTTATTGCTGGAAACCGACGCGCCGGATATG‑CCGCTCAACGGTTTTCAGGGGCAGCCTAACCGCCCGGAGCAGGCTGCCCGTGTGTTTGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAg                                                                                                                                                            <  1:248981/251‑1 (MQ=255)                                                                                          gttattaacccctacccccgcgCCAGTAAACCCCGCTATGTCATGGCAAAATTACCGCTGGCATCGTTATTGCTGGAACCCGACGCGCCGGATATG‑CCGCTCAGCGGTTTTCAGGGTCAGCCTAACCGCCCGGAGCAGGCTGCCCGTGTGTTTGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAGGCCGGATa                                                                                                                                                    <  2:234547/233‑1 (MQ=255)                                                                                            tACTATCACCTATCCACGCGCCAGTAAAACCCGCGATGTCATCGCAAAATTACCGCTGGCATCGTTATTGCTGGAAACCGACGCGCCGGATATG‑CCGCTCAACGGTTTTCAGGGGCAGCCTAACCGCCCGGAGCAGGCTGCCCGTGTGTTTGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACCCGTATACGTTGTTTAACGTGCCGTAGGCCGGATa                                                                                                                                                    >  2:262012/1‑249 (MQ=255)                                                                                                       aTCCACGCGCAAGTAAAACCCGGGATGTCATCGCAAAATTACCGCTGGCATCGTTATTGCTGGAAACCGACGCGCCGGATATG‑CCGCTCAACGGTTTTCAGGGGCAGCCTA‑CCGCCCGGAGCAGGCTGCCCGTGTGTTTGCCGTGCTGTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGTTCAcgcc                                                                                                                                       <  2:338213/250‑1 (MQ=255)                                                                                                                   gTAAAACCCGCGATGTCATCGCAAAATTACCGCTGGCATCGTTATTGCTGGAAACCGACGCGCCGGATATG‑CCGCTCAACGGTTTTCAGGGGCAGCCTAACCGCCCGGAGCAGGCTGCCCGTGTGTTTGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTCTAACGTGCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGt                                                                                                                           >  1:125018/1‑251 (MQ=255)                                                                                                                     aaaaCCCGCGATGTCATCGCAAAATTACCGCTGGCATCGTTATGGCTGGAAACCGACGCGCCGGATATG‑CCGATCAACGGTTTTCAGGGGCAGCCTAACCGCCCGGAGCAGGCTGCCCGTGTGTTTGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTg                                                                                                                         <  1:262012/251‑1 (MQ=255)                                                                                                                     aaaaCCCGCAATGTCATCGCAAAATTACCGCTGGCATCTTTATTGCTGGAAACCGACGCGCCGGATATG‑CGGATCAACGGTTTTCAGGGGCAGCCTAACCGCCCGGAGCAGGCTTCCCTTGTGTTTGCCGTGCGTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGGATCCGTTGTTTAACGGGCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGtt                                                                                                                          <  2:386524/250‑1 (MQ=255)                                                                                                                              gATGTCACCGCAAAATTCCCGCTGGCATCGTTATTCCTGAAAACCGACGCCCCTGATATG‑CCGCTCAACGGTTTTCAGGGGCAGTCTAACCGCCCTGAGCAGGCTGCCCGTGTGTTTGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTTTTTAACGTGCCGTAGGCCGTATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAAt                                                                                                                <  1:122120/251‑1 (MQ=255)                                                                                                                                 gTCATCGCAAAATTACCGCTGGCATCGTTATTGCTGGAAACCGACGCGCCGGATATG‑CCGCTCAACGGTTTTCAGGGGCAGCCTAACCGCCCGGAGCAGGCTGCCCGTGTGTTTGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAGGCCGGGTAAGGCGTTCACGCCGCATCCGGCAGTTGGAGCACAATGcc                                                                                                             >  2:312710/1‑251 (MQ=255)                                                                                                                                    aTCGAAAAATCACCGCTGGCATCGTTATTGCTGGAAACCGACGCGCCGGATATG‑CCGCTCAACGGTTTCCAGGGGCAGCCTAACCGCCCGGAGCAGGCTGCCCGTGTGTTTGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGa                                                                                                          <  2:222264/251‑1 (MQ=255)                                                                                                                                     ccGCAAAATTACCGCTGGCATCGTTATTGCTGGAAACCGACGCGCCGGATATG‑CCGCTCAACGGTTTTCAGGGGCAGCCTAACCGCCCGGAGCAGGCTGCCCGTGTGTTTGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGAt                                                                                                         <  1:142832/250‑1 (MQ=255)                                                                                                                                      cGCAAAATTACCGCTGGCATCGTTATTGCTGGAAACCGACGCGCCGGATATG‑CCGCTCAACGGTTTTCAGGGGCAGCCTAACCGCCCGGAGCAGGCTGCCCGTGTGTTTGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATg                                                                                                        >  1:5546/1‑251 (MQ=255)                                                                                                                                       gCAAAATTACCGCTGGCATCGTTATTGCTGGAAACCGACGCGCCGCATATG‑CCGCTCTACGGTTTTCAGGGGCAGCCTAACCGCCCGGAGCAGGCTGCCCGTGTGTTTGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGGAGGCCGGATAAGGCGTTCACGCCGCATCAGGAAGTTGGCGCACAATACCTGATgg                                                                                                       >  1:157695/1‑250 (MQ=255)                                                                                                                                         aaaaTTACCGCTGGCATCGTTATTGCTGGAAACCGACGCGCCGGATATG‑CCGCTCAACGGTTTTCAGGGGCAGCCTAACCGCCCGGAGCAGGCTGCCCGTGTGTTTGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAAGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCgc                                                                                                     >  1:346477/1‑250 (MQ=255)                                                                                                                                          aaatatccGCTGGCATCGTTATTGCGGGAAACCGACGCGCCGGATATG‑CCGCTCAACGGTTTTCAGGGGCAGCCTAACCGCCCGGAGCAGGCTGCCCGTGTGTTTTCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGAc                                                                                                    <  1:449715/245‑1 (MQ=255)                                                                                                                                          aaaTTACCGCTGGCATCGTTATTGCTGGAAACCGACGCGCTGGATATG‑CCGATCAACGGTTTTCAGGGGCAGCCTAACCGCCCGGAGCAGGTTGCTCGTGGGTTTGCCGTGCTTTGCGAGTTGCGCAGGGAACCGGGGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGAc                                                                                                    <  1:449700/251‑1 (MQ=39)                                                                                                                                           aaTTACCGCTGGCATCGTTATTGCTGGAAACCGACGCACCGGATATG‑CCGCTCAACGGTTTTCAGGGGCAGCCTAACCGCCCGGAGCAGGCTGCCCGTGTGTTTGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACg                                                                                                   >  2:186075/1‑251 (MQ=255)                                                                                                                                                 cGCTGGCATCGTTATTGCTGGAAACCGACGCGCCGGATATG‑CCGCTCAACGGTTTTCAGGGGCAGCCTAACCGCCCGGAGCAGGCTGCCCGTGTGTTTGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAAc                                                                                             >  1:292020/1‑251 (MQ=255)                                                                                                                                                  gCTGGCATCGTTATTGCTGGAAACCGACGCGCCGGATAGG‑CCGCTCAACGGTTTTCAGGGGCAGCCTAACCGCCCGGAGCAGGCGGCCCGTGTGTTTGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAAcg                                                                                            <  1:164755/251‑1 (MQ=255)                                                                                                                                                       caccgttatgtcaggAAACCGTAGCGCCGG‑TATGGCCGTTCAACGGTTTCCAGGGGGAGCCGAACCGTCCGGAGTAGGCTGCCAGTGTGTTTTCCGTGCTTTGCAAGTGGCGCCGGGAACCGGCGGATGATATTGCCCCAGCGTTGCTTAATAACACGTATACGTTGTTGAACGTGCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATTCTGCGCTTAAcccctct                                                                                       <  2:434392/238‑7 (MQ=19)                                                                                                                                                            ttATTGCTGGAAACCGACGCGCCGGATATG‑CCGCTCAACGGTTTTCAGGGGCAGCCTAACCGCCCGGAGCAGGCTGCCCGTGTGTTTGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATc                                                                                   >  1:95147/1‑250 (MQ=255)                                                                                                                                                                tGCTGGAAACCGACGCGCCGGATATG‑CCGCTCAACGGTTTTCAGGGGCAGCCTAACCGCCCGGAGCAGGCTGCCCGTGTGTTTGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGcc                                                                              >  1:316812/1‑251 (MQ=255)                                                                                                                                                                  cTGGAAACCGACGCGCCGGATATG‑CCGCTCAACGGTTTTCAGGGGCAGCCTAACCGCCCGGAGCAGGCTGCCCGTGTGTTTGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGGGATTGCGCAAGCGTTGCTTAATAACACGTATACGATGTTTAACGTTCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTTGCGCACAAAGCCTGCTGCGACGCTTTACGCGTCTTATCATGCCt                                                                             >  2:80746/1‑250 (MQ=38)                                                                                                                                                                     gAAACAGACGCGCCGGATATG‑CCGCTCAACGGTTTTCAGGGGCAGCCTAACCGCCCGGAGCAGGCAGCCCGTGTGTTTGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGCTCACGCCGCATCCGGCAGTTGGCACACCACTCCTGGTGCGACGCTTAACGCGCCTTCTCATGCCtcc                                                                           >  2:437841/1‑247 (MQ=255)                                                                                                                                                                       aaCCGACGCGCCGGATATG‑CCGCTCAACGGTTTTCAGGGGCAGCCTAACCGCCCGGAGCAGGCTGCCCGTGTGTTTGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGt                                                                       >  1:214311/1‑251 (MQ=255)                                                                                                                                                                               cgcCGGATATG‑CCGCTCAACGGTTTTCAGGGGCAGCCTAACCGCCCGGAGCAGGCTGCCCGTGTGTTTGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTTCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCg                                                               >  1:46862/1‑251 (MQ=255)                                                                                                                                                                                 ccGGATATG‑CCGCTCAACGGTTTTCAGGGGCAGCCTAACCGCCCGGAGCAGGCTGCCCGTGTGTTTGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAAACCGTCTTATCCTGCCTACCGGTTTGTGCCGac                                                             >  2:405635/1‑250 (MQ=255)                                                                                                                                                                                            cgcccAACGGTTTTCAGGGGCAGCCTAACCGCCCGGAGCAGGCTGCCCGTGTGTTTGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAAcgc                                                                                           <  2:27595/207‑1 (MQ=255)                                                                                                                                                                                            cGCTCAACGGTTTTCAGGGGCAGCCTAACCGCCCGGAGCAGGCTGCCCGTGTGTTTGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAAcgc                                                                                           >  1:27595/1‑211 (MQ=255)                                                                                                                                                                                               tCAACGGTTTTCAGGGGCAGCCTAACCGCCCGGAGCAGGCTGCCCGTGTGTTTGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCACGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGCCGCTTACCGCGTCTTATCTTGCCTACCGGTTTGTGCCGCACCGTAGGCCGGa                                                 >  1:285355/1‑250 (MQ=38)                                                                                                                                                                                                    ggTTTTCAGGGGCAGCCTAACCGCCCGGAGCAGGCTGCCCGTGTGTTTGCCGTGCTTTGCGAGTTGCGCCGGGCACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGCTAAGGc                                           >  1:448718/1‑251 (MQ=255)                                                                                                                                                                                                    ggTTTTCAGGGGCAGCCTAACAGCCCGGAGCAGGCTGCCCGTGTGTTTGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGCTGAGATTGCGCAAGCGTTGCTTAATAACCCGAATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGTTCACGCCGCCTCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAAACGAAGGACGGATAAggg                                           >  2:42904/1‑250 (MQ=37)                                                                                                                                                                                                        ttCAGGGGCAGCCTAACCGCCCGGAGCAGGCTGCCCGTGTGTTTGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGtt                                        <  1:135965/250‑1 (MQ=255)                                                                                                                                                                                                          cAGGGGCAGCCTAACCGCCCGGAGCAGGCTGCCCGTGTGTTTGCCGTGCTTTGCGAGTTGCGCCGGGAACCAGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGAGCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAAGACGGATAATGCGTTCAc                                     >  1:168467/1‑251 (MQ=255)                                                                                                                                                                                                                            cgggAGCAGGGGGCCCGTGTGTTTGCGGGGCTTGGCGGGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACTTATACGTGGTTTAACGTGCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTgg                   <  2:39937/249‑1 (MQ=21)                                                                                                                                                                                                                                         cccGTGTGTTTGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGtt                     >  1:318273/1‑236 (MQ=255)                                                                                                                                                                                                                                         cccGTGTGTTTGCCGTGCTTTGCGAGTTGCGCCGGGAAACGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTg      >  1:354192/1‑251 (MQ=255)                                                                                                                                                                                                                                           cgtttgtTTGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGAt    <  1:311106/247‑1 (MQ=255)                                                                                                                                                                                                                                           cGTGTGTTTGCCGGGCTTTGTGAGTTGCGCCGGGAACCGGCGGAGGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTGATCGTGCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACAGTAGGCCGGATAAGGTGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGAt    <  1:346223/251‑1 (MQ=21)                                                                                                                                                                                                                                             tgtgtTTGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGc  >  1:342627/1‑251 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                     TACCGCGCACTGGCGTGGTTCACGGTTTTTCCGGCAGCCTGCAACAGGCCGAACGGTTTGTACAGCTGGGCTACAAAATTGGCGTAGGCGGTACTATCACCTATCCACGCGCCAGTAAAACCCGCGATGTCATCGCAAAATTACCGCTGGCATCGTTATTGCTGGAAACCGACGCGCCGGATATG‑CCGCTCAACGGTTTTCAGGGGCAGCCTAACCGCCCGGAGCAGGCTGCCCGTGTGTTCGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGC  >  NC_000913/4613929‑4614414

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑