Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,592,082	G→A	L142L (CTG→CTA)	tsr →	methyl‑accepting chemotaxis protein Tsr

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,592,082	0	G	A	100.0%	128.0 / NA	41	L142L (CTG→CTA)	tsr	methyl‑accepting chemotaxis protein Tsr
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (24/17);  total (24/17)

GTTGCAGACGCGTAACACCCTCAACCGCGCGGGTATCCGCTACATGATGGATCAGAATAATATTGGTAGCGGTTCAA‑CCGTTGCTGA‑GCTGATGGAGAGTGCCAGTATTTCGCTGAAACAGGCGGAAAAAAACTGGGCGGATTACGAAGCGTTGCCGCGTGACCCGCGTCAGAGCACCGCCGCAGCGGCAGAGATCAAACGTAATTACGATATTTATCACAATGCGCTGGCGGAGCTGATCCAACTGTTAGGTGCAGGCAAAATCAACGAGTTCTTTGATCAGCCGACCCAGG‑GATATCAGGACGGTTTCGAGAAGCAGTATGTGGCTTACATGGAGCAAAACGATCGGCTCCATGATATCGCCGTCAGCGATAACAATGCCTCCTACAGCCAGGCGATGTGGATTCTGGTGGGCGTGATGATCGTCGTACTGGCGGTCATCTTCGCCGTCTGGTTCGGTATTAAAGCCTCGCTGGTAG  >  NC_000913/4591836‑4592314                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                          gttgCAGACGCGTAACACCCTCAACCGCGCGGGTATCCGCTACAGGATGGATCAGAATAATATTGGTAGCGGTTCAA‑CCGTTGCTGA‑GCTGATGCAGAGTGCCAGTATTTCGCTGAAACAGGCGGAAAAAAACTGGGCAGATTACGAAGCGTTGCCGCGTGACCCGCGTCAGAGCACCGCCGCAGCGGCAGAGATCAAACGTAATTACGATATTTATCACAATGCGCTGGCGGAGCTGATCCAACTATTa                                                                                                                                                                                                                                        <  1:19553/250‑1 (MQ=255)     cAGACGCGTAACACCCTCAACCGCGCGGGTATCCGCTACATGATGGATCAGAATAATATTGGTAGCGGTTCAA‑CCGTTGCTGA‑GCTGATGCAGAGTGCCAGTATTTCGCTGAAACAGGCGGAAAAAAACTGGGCAGATTACGAAGCGTTGCCGCGTGACCCGCGTCAGAGCACCGCCGCAGCGGCAGAGATCAAACGTAATTACGATATTTATCACAATGCGCTGGCGGAGCTGATCCAACTATTAGGTGc                                                                                                                                                                                                                                   >  1:212250/1‑251 (MQ=255)                 acCCTCAACCGCGGGGGTATCCGCGACATGATGGATAAGAATAATATTGGTGGTGGTTCAC‑CCGTTGCTGA‑GCTGATGCAGAGTGCCAGTATTTCGCTGAAACAGGCGGAAAAAAACTGGGCAGTTTACGAAGCGTTGCCGCGTGACCCTCGTCAGAGCACCGCCGCAGCGGCAGAGATCAAACGTAATTACGATATTTATCACAATGCGCTGGCGGAGCTGATCCAACTATTAGGTGCAGGCAAAATCaa                                                                                                                                                                                                                       <  1:229606/251‑1 (MQ=255)                              agggaaTCCGCTACATGATGGATCAGAGTAATATTGGTAGCGGTTCAA‑CCGTTGCTGA‑GCTGATGCAGAGTGCCAGTATTTCGCTGAAACAGGCGGAAAAAAACTGGGCAGATTACGAAGCGTTGCCGCGTGACCCGCGTCAGAGCACCGCCGCAGCGGCAGAGATAAAACGTAATGACGATATTTATCACAATGCGCTGGCGGAGATGATCCACCTATTAGGTGCAGGCAAAATCAACGAGTACTTTGAt                                                                                                                                                                                                          >  2:104732/6‑251 (MQ=255)                                      cGCTACATGATGGATCAGAATAATATTGGTAGCGGTTCAA‑CCGTTGCTGA‑GCTGATGCAGAGTGCCAGTATTTCGCTGAAACAGGCGGAAAAAAACTGGGCAGATTACGAAGCGTTGCCGCGTGACCCGCGTCAGAGCACCGCCGCAGCGGCAGAGATCAAACGTAATTACGATATTTATCACAATGCGCTGGCGGAGCTGATCCAACTATTAGGTGCAGGCAAAATCAACGAGTTCTTTGATCAGCCGAc                                                                                                                                                                                                  >  1:288509/1‑251 (MQ=255)                                       gCTAAATGTTGGATCAGAAAAATATGGGTAGCG‑TTCAAACCGTTGCTGA‑GCGGATGCAGAGTGCCAGTATTTCGCTGAAACAGGCGGAAAAAAACTGGGCAGATGACGAAGGGTGGCCGCGTGCCCCGCGTCAGAGCACCGCCGCAGCGGCAGAGATCAAACGTAATTACGATATTTATCACAATGCGCTGGCGGAGCTGATCCAACTATTAGGTGCAGGCAAAATCAACGAGTTCTTTGATCAGCCGAcc                                                                                                                                                                                                 <  2:135233/251‑1 (MQ=255)                                         tACATGATGGATCAGAATAATATTGGTAGCGGTTCAA‑CCGTTGCTGA‑GCTGATGCAGAGTGCCAGTATTTCGCTGAAACAGGCGGAAAAAAACTGGGCAGATTACGAAGCGTTGCCGCGTGACCCGCGTCAGAGCACCGCCGCAGCGGCAGAGATCAAACGTAATTACGATATTTATCACAATGCGCTGGCGGAGCTGATCCAACTATTAGGTGCAGGCAAAATCAACGAGTTCTTTGATCAGCCGAccc                                                                                                                                                                                                >  1:69395/1‑250 (MQ=255)                                              gttgGATCAAAATAAATTTGGAAGCGGTTCAA‑CCGT‑GGTGAAGCGGATGCAGAGTGCCAGTATTTCGTTGAACAAGGAGAAAAAAAACTGGGAAGATTCGGAGGCGTGGCCGCGTAACCCGCGTCAAAGCACCGCCGCAGCGGCAGAGATCAAACGTAATTACGATATTTATCACAATGCGCTGGCGGAGCTGATCCAACGATTAGGTGCATGCAAAATCAACGAGTTCTTTGATCAGCCGACCCAGG‑Ga                                                                                                                                                                                          <  2:305767/248‑1 (MQ=255)                                               agggATCAGAATAATATTGGAAGCGGTTAAA‑CAGTTGCTGG‑GCGGAGGAAGAGTGCAAGTATTTAGCTGAAAAGGGAGGAAAAAAATGGGGAAGATTAGGAAGCGTTGCCGATTGACCCGCGTCAGAGAACCGTCGCAGCGGCAGAGATCAAACGTAATTACGATATTTATCACAATGCGCTGGCGGAGCTGATCCAACTATTAGGTGCAGGCAAAATCAACGAGTGCTTTGATCAGCCGACCCAGG‑Gata                                                                                                                                                                                        <  1:336104/249‑1 (MQ=255)                                                  gataaGAATAATATTGGTAGCGGTTCAA‑CCGTTGCTGA‑GCTGATGCAGAGTGCCAGTATTTCGCTGCAACAGGCGGACAAAAACTGGGCAGATTACGAAGCGTTGCCGCGTGACCCGCGTCAGAGCACCGCCGCAGCGGCAGAGATCAAACGTAATTACGATATTTATCACAATGCGCTGGCGGAGCTGATCCCACTATTAGGTGCAGGCAAAATCAACGGGTTCTTTGCTCAGCCCACCAAGG‑GATATcg                                                                                                                                                                                     >  2:222675/5‑250 (MQ=255)                                                  gATCAGAATAATATTGGTAGCGTTTCAA‑CCGTTGCTGA‑GCTGATGCAGAGTGCCAGTATTTCGCTGAAACAGGCGGAAAAAAACTGGGCAGATTACGAAGCGTTGCCGCGTGACCCGCGTCAGAGCACCGCCGCAGCGGCAGAGATCAAACGTAATTACGATATTTATCACAATGCGCTGGCGGAGCTGATCCAACTATTAGGTGCAGGCAAAATCAACGAGTTCTTTGATCAGCCGACCCAGG‑GATATCa                                                                                                                                                                                     <  1:224760/251‑1 (MQ=255)                                                      aGAATAATATTGGTAGCGGTTCAA‑CCGTTGCTGA‑GCTGATGCAGAGTGCCAGTATTTCGCTGAAACAGGCGGAAAAAAACTGGGCAGATTACGAAGCGTTGCCGCGTGACCCGCGTCAGAGCACCGCCGCAGCGGCAGAGATCAAACGTAATTACGATATTTATCACAATGCGCTGGCGGAGCTGATCCAACTATTAGGTGCAGGCAAAATCAACGAGTTCTTTGATCAGCCGACCCCGG‑GATATCAGGAc                                                                                                                                                                                 >  1:350704/1‑251 (MQ=255)                                                       gAATAATATTGGTAGCGGTTCAA‑CCGTTGCTGA‑GCTGATGCAGAGTGCCAGTATTTCGCTGAAACAGGCGGAAAAAAACTGGGCAGATTACGAAGCGTTGCCGCGTGACCCGCGTCAGAGCACCGCCGCAGCGGCAGAGATCAAACGTAATTACGATATTTATCACAATGCGCTGGCGGAGCTGATCCAACTATTAGGTGCAGGCAAAATCAACGAGTTCTTTGATCAGCCGACCCAGG‑GATATCAGGACg                                                                                                                                                                                >  1:58504/1‑251 (MQ=255)                                                         aaaataGTGGTAGCGGTCCAA‑CCGTGGCGGA‑GCTGAGGCAGGGGGCCAGTATTTCGCTGAAACAGGCGAAAAAAAACGGGGCAGATGACGAAGCGTGCCCGCGTGCCCCGCGCCAGAGCACCGCCGCAGCGGCAGAGATCAAACGTAATTACGATATTTATCACAATGCGCTGGCGGAGCTGATCCAACTATTAGGTGCAGGCAAAATCAACGAGTTCTTTGATCAGCCGACCCAGG‑GATATCAGGACGGt                                                                                                                                                                              <  2:87268/249‑1 (MQ=255)                                                                  gTAGCGGTTCAA‑CCGTTGCTGA‑GCTGATGCAGAGTGCCAGTATTTCGCTGAAACAGGCGGAAAAAAACTGGGCAGATTACGAAGCGTTGCCGCGTGACCCGCGTCAGAGCACCGCCGCAGCGGCAGAGATCAAACGTAATTACGATATTTATCACAATGCGCTGGCGGAGCTGATCCAACTATTAGGTGCAGGCAAAATCAACGAGTTCTTTGATCAGCCGACCCAGG‑GATATCAGGACGGTTTCgaga                                                                                                                                                                       >  1:451408/1‑249 (MQ=255)                                                                                cGTTGCTGA‑GCTGATGCAGAGTGCCAGTATTTCGCTGAAACAGGCGGCAAAAAACTGGGCAGATTACGAAGCGTTGCCGCGTGACCCGCGTCAGAGCACCGCCGCAGCGGCAGAGATCAACCGTAATTACGGTATTTATCACACTGCGCTGGCGGAGCTGATCCCACTATTAGGTGCAGGCACCATCCACGGGTTCTTTGATCGGCAGAACCAGGCGA‑CTCAGGTGGGTTTCGAGAAGCAGTATGTGGCtt                                                                                                                                                        >  2:376678/1‑251 (MQ=255)                                                                                    gctga‑gctgaTGCAGAGTGCCAGTATTTCGCTGAAACAGGCGGAAAAAAACTGGGCAGATTACGAAGCGTTGCCGCGTGACCCGCGTCAGAGCACCGCCGCAGCGGCAGAGATCAAACGTAATTACGATATTTATCACAATGCGCTGGCGGAGCTGATCCAACTATTAGGTGCAGGCAAAATCAACGAGTTCTTTGATCAGCCGACCCAGG‑GATATCAGGACGGTTTCGAGAAGCAGTATGTGGCTTACAt                                                                                                                                                    >  1:391541/1‑251 (MQ=255)                                                                                                    agTGCCAGTATTTCGCTGAAACAGGCGGAAAAAAACTGGGCAGATTACGAAGCGTTGCCGCGTGACCCGCGTCAGAGCACCGCCGCAGCGGCAGAGATCAAACGTAATTACGATATTTATCACAATGCGCTGGCGGAGCTGATCCAACTATTAGGTGCAGGCAAAATCAACGAGTTCTTTGATCAGCCGACCCAGG‑GATATCAGGACGGTTTCGAGAAGCAGTATGTGGCTTACATGGAGCAGAACGAt                                                                                                                                       >  2:18625/1‑249 (MQ=255)                                                                                                      tGCCAGTATTTCGCTGAAACAGGCGGAAAAAAACTGGGCAGATTACGAAGCGTTGCCGCGTGACCCGCGTCAGAGCACCGCCGCAGCGGCAGAGATCAAACGTAATTACGATATTTATCACAATGCGCTGGCGGAGCTGATCCAACTATTAGGTGCAGGCAAAATCAACGAGTTCTTTGATCAGCCGACCCAGG‑GATATCAGGACGGTTTCGAGAAGCAGTATGTGGCTTACATGGAGCAAAACGATCGGc                                                                                                                                   >  1:391589/1‑251 (MQ=255)                                                                                                       gCCAGTATTTCGCTAAAACAGGCGGAAAAAAACGGGGCAGATTACGAAGCGTTGCCGCGTGACCCGCGTCAGAGCACCGCCGCAGCGGCAGAGATCAAACGTAATTACGATATTTATCACAATGCGCTGGCGGAGCTGATCCAACTATTAGGTGCAGGCAAAATCAACGAGTTCTTTGATCAGCCGACCCAGG‑GATATCAGGACGGTTTCGAGAAGCAGTATGTGGCTTACATGGAGCAAAACGATCGGCt                                                                                                                                  <  1:424314/251‑1 (MQ=255)                                                                                                           gaagttctcggAAACAGGGGGAAAAAAACTGGGCATCTGTCGAAGCGTTGCCGGTTGACCCGTGTCAGATCACCGCCGCAGCGGCAGAGATCAAACGTAATTACGATATTTATCACAAGGCGCTGGTGGAGCTGATCCAACTATTAGGTGCAGGCAAGAGCGACGAGTTCGTTGGTCAGCCGACCCAGG‑GATATCTGTACGGTTTCGAGAAGCAGTATGTGGCTTACATGGAGCAAAACGATCGGCTctat                                                                                                                              <  1:26425/241‑4 (MQ=255)                                                                                                                                     aaCTGGGCAGATTACGAAGCGTTGCCGCGTGACCCGCGTCAGAGCACCGCCGCAGCGGCAGAGTTCAAACGTAATTACGATATTTATCACAATGCGCTGGCGGAGCTGATCCAACTATTAGGTGCAGGCAAAATCAACGAGTTCTTTGATCAGCCGACCCAGG‑GATATCAGGACGGTTTCGAGAAGCAGTATGTGGCTTACATGGAGCAAAACGATCGGCTCTATGATATCGCCGTCAGCGATAACAATGc                                                                                                    >  1:140855/1‑251 (MQ=255)                                                                                                                                      aCTGGGCAGATTACGAAGCGTTGCCGCGTGACCCGCGTCAGAGCACCGACGCAGAGGCAGAGATCAAACGTAATTACGATATTTATCACAATGAGCCGGCGGAGCTGATCCAACTATAAGGTGCAGACAAAATCAACGAGTTCTTTGATCAGCCGACCCAGG‑GATATCAGGACGGTTTCGAGAAGCAGTATGTGGCTTACATGGAGCAAAACGATCGGCTCTATGATATCGCCGTCAGCGATAACAATGcc                                                                                                   >  1:465826/1‑251 (MQ=255)                                                                                                                                                 tACGAAGCGTTGCCGCGTGACCCGCGTCAGAGCACCGCCGCAGCGGCAGAGATCAAACGTAATTACGATATTTATCACAATGCGCTGGCGGAGCTGATCCAACTATTAGGTGCAGGCAAAATCAACGAGTTCTTTGATCAGCCGACCCAGG‑GATATCAGGACGGTTTCGAGAAGCAGTATGTGGCTTACATGGAGCAAAACGATCGGCTCTATGATATCGCCGTCAGCGATAACAATGCCTCCTACAGcc                                                                                         <  1:453242/250‑1 (MQ=255)                                                                                                                                                    gAAGCGTTGCCGCGTGACCCGCGTCAGAGCACCGCCGCAGCGGCAGAGATCAAACGTAATTACGATATTTATCACAATGCGCTGGCGGAGCTGATCCAACTATTAGGTGCAGGCAAAATCAACGAGTTCTTTGATCAGCCGACCCAGG‑GATATCAGGACGGTTTCGAGAAGCAGTATGTGGCTTACATGGAGCAAAACGATCGGCTCTATGATATCACCGTCAGCGATAACAATGCCTCCTACAGCCAGGc                                                                                     >  1:92280/1‑251 (MQ=255)                                                                                                                                                        cGTTGCCGCGTGACCCGCGTCAGAGCACCGCCGCAGCGGCAGAGATCAAACGTAATTACGATATTTATCACAATGCGCTGGCGGAGCTGATCCAACTATTAGGTGCAGGCAAAATCAACGAGTTCTTTGATCAGCCGACCCAGG‑GATATCAGGACGGTTTCGAGAAGCAGTATGTGGCTTACATGGAGCAAAACGATCGGCTCTATGATATCGCCGTCAGCGATAACAATGCCTCCTACAGCCAGGCgttg                                                                                 >  1:214662/1‑248 (MQ=255)                                                                                                                                                         gtggCCGCGTGCCCCGCGTCAGAGCACCGCCGCAGCGGCAGAGATCAAACGTAATTACGATATTTATCACAATGCGCTGGCGGAGCTGATCCAACTATTAGGTGCAGGCAAAATCAACGAGTTCTTTGATCAGCCGACCCAGG‑GATATCAGGACGGTTTCGAGAAGCAGTATGTGGCTTACATGGAGCAAAACGATCGGCTCTATGATATCGCCGTCAGCGATAACAATGCCTCCTACAGCCAGGCGAtg                                                                                 <  1:469759/247‑1 (MQ=255)                                                                                                                                                         gTTGCCGCGTGACCCGCGTCAGAGCACCGCCGCAGCGGCAGAGATCAAACGTAATTACGATATTTATCACAATGCGCTGGCGGAGCTGATCCAACTATTAGGTGCAGGCAAAATCAACGAGTTCTTTGATCAGCCGACCCAGG‑GATATCAGGACGGTTTCGAGAAGCAGTATGTGGCTTACATGGAGCAAAACGATCGGCTCTATGATATCGCCGTCAGCGATAACAATGCCTCCTACAGCCAGGCGAtgt                                                                                >  1:102127/1‑251 (MQ=255)                                                                                                                                                               gcgTGACCCGCGTCAGAGCACCGCCGCAGCGGCAGAGATCAAACGTAATTACGATATTTATCACAATGCGCTGGCGGAGCTGATCCAACTATTAGGTGCAGGCAAAATCAACGAGTTCTTTGATCAGCCGACCCAGG‑GATATCAGGACGGTTTCGAGAAGCAGTATGTGGCTTACATGGAGCAAAACGATCGGCTCTATGATATCGCCGTCAGCGATAACAATGCCTCCTACAGCCAGGCGATGTGGATTc                                                                          >  1:149299/1‑251 (MQ=255)                                                                                                                                                                  tGACCCGCGTCAGAGCACCGCCGCAGCGGCAGAGATCAAACGTAATTACGATATTTATCACAATGCGCTGGCGGAGCTGATCCAACTATTAGGTGCAGGCAAAATCAACGAGTTCTTTGATCAGCCGACCCAGG‑GATATCAGGACGGTTTCGAGAAGCAGTATGTGGCTTACATGGAGCAAAACGATCGGCTCTATGATATCGCCGTCAGCGATAACAATGCCTCCTACAGCCAGGCGATGTGGATTCtgg                                                                       >  1:405953/1‑251 (MQ=255)                                                                                                                                                                    ccccGCGTCAGAGCACCGCCGCAGCGGCAGAGATCAAACGTAATTACGATATTTATCACAATGCGCTGGCGGAGCTGATCCAACTATTAGGTGCAGGCAAAATCAACGAGTTCTTTGATCAGCCGACCCAGG‑GATATCAGGACGGTTTCGAGAAGCAGTATGTGGCTTACATGGAGCAAAACGATCGGCTCTATGATATCGACGTCAGCGATAACAATGCCTCCTACAGCCAGGCGATGTGGATTCtggtg                                                                     <  2:340304/250‑1 (MQ=255)                                                                                                                                                                        gcgacagacaaCCGCCGCAGCGGCAGAGATCAAACGTAATTACGATATTTATCACAATGCGCTGGCGAAGCTGATCCAACTATTAGGTGCAGGCAAAATCAACGAGTTCTTTGATCAGCCGACCCAGG‑GATATCAGGACGGTTTCGAGAAGCAGTATGTGGCTTACATGGAGCAAAACGATCGGCTCTATGATATCGCCGTCAGCGATAACAATGCCTCCTACAGCCAGGCGATGTGGATTCTGGTGGGCg                                                                 <  2:70547/241‑1 (MQ=255)                                                                                                                                                                                 cACCGCCGCAGCGGCAGAGATCAAACGTAATTACGATATTTATCACAATGCGCTGGCGGAGCTGATCCAACTATTAGGTGCAGGCAAAATCAACGAGTTCTTTGATCAGCCGACCCAGG‑GATATCAGGACGGTTTCGAGAAGCAGTATGTGGCTTACATGGAGCAAAACGATCGGCTCTATGATATCGCCGTCAGCGATAACAATGCCTCCTACAGCCAGGCGATGTGGATTCTGGTGGGCGTGATGAtcg                                                        >  1:79411/1‑251 (MQ=255)                                                                                                                                                                                  aCCGCCGCAGCGGCAGAGATCAAACGTAATTACGATATTTATCCCAATGCGCGGGCGGAGCTGATCCAACTATAAGGTGAAGGCAAAATCAACGAGTTCTTTGATCAGCCGACCCGGG‑GATATAAGGCGGGTTTCGAGAAGCAGTATGGGGCTTACATGGAGAAAAACGATGGGCTCAATGATATGGCAGTCAGCGATAACAATGCATCCTAAAGCCAGGCGATGTGGATCTTGGTGGGCgggagaatggg                                                       >  1:127860/1‑241 (MQ=255)                                                                                                                                                                                           gCGGCAGAGATCAAACGTAATTACGATATTTATCACAATGCGCTGGCGGAGCTGATCCAACTATTAGGTGCAGGCAAAATCAACGAGTTCTTTGATCAGCCGACCCAGG‑GATATCa                                                                                                                                                                                     <  1:191423/116‑1 (MQ=255)                                                                                                                                                                                           gCGGCAGAGATCAAACGTAATTACGATATTTATCACAATGCGCTGGCGGAGCTGATCCAACTATTAGGTGCAGGCAAAATCAACGAGTTCTTTGATCAGCCGACCCAGG‑GATATCa                                                                                                                                                                                     >  2:191423/1‑116 (MQ=255)                                                                                                                                                                                                 gagaTCAAACGTAATTACGATATTTATCACAATGCGCTGGCGGAGCTGATCCAACTATTAGGTGCAGGCAAAATCAACGAGTTCTTTGATCAGCCGACCCAGG‑GATATCAGGACGGTTTCGAGAAGCAGTATGTGGCTTACATGGAGCAAAACGATCGGCTCTATGATATCGCCGTCAGCGATAACAATGCCTCCTACAGCCAGGCGATGTGGATTCTGGTGGGCGTGATGATCGTCGTACTGGCGGTCAt                                        <  2:102127/251‑1 (MQ=255)                                                                                                                                                                                                             aaTTACGATATTTATCACAATGCGCTGGCGGAGCTGATCCAACTATTAGGTGCAGGCAAAATCAACGAGTTCTTTGATCAGCCGACCCAGG‑GATATCAGGACGGTTTCGAGAAGCAGTATGTGGCTTACATGGAGCAGAACGATCGGCTCTATGATATCGCCGTCAGCGATAACAATGCCTCCTACAGCCAGGCGATGTGGATTCTGGTGGGCGTGATGATCGTCGTACTGGCGGTCATCTTCGCCGTCt                             <  1:18625/250‑1 (MQ=255)                                                                                                                                                                                                              aTTACGATATTTATAACAATGCGCTGGCGGAGCTGATCCAACTATTAGGTGCAGGCAAAATCAACGAGTTCTTTGATCAGCCGACCCAGG‑GATATCAGGACGGTTTCGAGAAGCAGTATGTGGCTTACATGGAGCAAAACGATCGGCTCTATGATATCGCCGTCAGCGATAACAATGCCTCCTACAGCCAGGCGATGTGGATTCTGGTGGGCGTGATGATCGTCGTACTGGCGGTTCTCTTTGCCGTCTgg                           >  1:155532/1‑251 (MQ=255)                                                                                                                                                                                                                          aTCACAATGCGCTGGCGGAGCTGATCCAACTATTAGGTGCAGGCAAAATCAACGAGTTCTTTGATCAGCCGACCCAGG‑GATATCAGGACGGTTTCGAGAAGCAGTATGTGGCTTACATGGAGCAAAACGATCGGCTCTATGATATCGCCGTCAGCGATAACAATGCCTCCTACAGCCAGGCGATGTGGATTCTGGTGGGCGTGATGATCGTCGTACTGGCGGTAATCTTCGCCGTcctggtcggtgttaa                >  1:278934/1‑236 (MQ=255)                                                                                                                                                                                                                                       ggCGGAGCTGATCCAACTATTAGGTGCAGGCAAAATCAACGAGTTCTGTGATCAGCCGACCCAGG‑GATATCAGGACGGTTTCGAGAAGCAGTATGTGGCTTACATGGAGCAAAACGATCGGCTCTATGATATCGCCGTCAGCGATAACAATGCCTCCTACAGCCAGGCGATGTGGATTCTGGTGGGCGTGATGATCGTCGTACTGGCGGTCATCTTCGCCGTCTGGTTCGGTATTAAAGCCTCGCTGGTAg  <  1:162056/251‑1 (MQ=255)                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                          GTTGCAGACGCGTAACACCCTCAACCGCGCGGGTATCCGCTACATGATGGATCAGAATAATATTGGTAGCGGTTCAA‑CCGTTGCTGA‑GCTGATGGAGAGTGCCAGTATTTCGCTGAAACAGGCGGAAAAAAACTGGGCGGATTACGAAGCGTTGCCGCGTGACCCGCGTCAGAGCACCGCCGCAGCGGCAGAGATCAAACGTAATTACGATATTTATCACAATGCGCTGGCGGAGCTGATCCAACTGTTAGGTGCAGGCAAAATCAACGAGTTCTTTGATCAGCCGACCCAGG‑GATATCAGGACGGTTTCGAGAAGCAGTATGTGGCTTACATGGAGCAAAACGATCGGCTCCATGATATCGCCGTCAGCGATAACAATGCCTCCTACAGCCAGGCGATGTGGATTCTGGTGGGCGTGATGATCGTCGTACTGGCGGTCATCTTCGCCGTCTGGTTCGGTATTAAAGCCTCGCTGGTAG  >  NC_000913/4591836‑4592314

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑