| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,591,677 | T→C | I7I (ATT→ATC) | tsr → | methyl‑accepting chemotaxis protein Tsr |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,591,677 | 0 | T | C | 86.7% | 28.2 / ‑3.4 | 15 | I7I (ATT→ATC) | tsr | methyl‑accepting chemotaxis protein Tsr |
| Reads supporting (aligned to +/- strand): ref base T (1/1); new base C (4/9); total (5/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.31e-01 | |||||||||||
TAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCAGGCCGAAAATCTTGCATCGGTCCACAGGAAAGAGAAACCATGTTAAAACGTATCAAAATTGTGACCAGCTTACTGCTGGTTTTGGCCGTTTTTGGCCTTTTACAACTGACATCAGGCGGTCTGTTCTTTAATGCCTTAAAGAATGACAAAGAAAATTTCACTGTTTTACAAACCATTCGCCAGCAGCAATCCACGCTGAATGGCAGCTGGGTCGCGTTGTTGCAGACGCGTAACACCCTCAACCGCGCGGGTATCCGCTACATGATGGATCAGAATAATATTGGT > NC_000913/4591486‑4591902 | tAAATATGTGATTCCTATCACATATTTCTATTGTGAATAATTTATGTAAATAATGACTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCAGGCCGAAAATCTTGCATCGGTCCACAGGAACGAGAAACCATGTTACAACGTATCAAAATTGTGACCAGCTTActg > 1:41130/1‑207 (MQ=255)gaaaTATGTGATGCATATATAATATTTATAGTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATGCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCAGGCCGAAAATCTTGCATCGGTCCACAGGAAAGAGAAACCATGTTAAAACGTATCAAAATTGTGACCAGCTTActg < 2:41130/206‑1 (MQ=255) ccatggatggcgggttacaccttttCATAAAGTTTTTGCTTTCCAGGCCGAAAATCTTGCATCGGTCCACAGGAAAGAGAAACCATGTTAAAACGTATCAAGATCGTGACCAGCTTACTGCTGGTTTTGGCCGTTTTTGGCCTTTTACAACTGACATCAGGCGGTCTGTTCTTTAATGCCTTAAAGAATGACAAAGAAAATTTCACTGTTTTACAAACCATTCGCCAGCAGCAATCCACGCTGAATGGCAg < 1:144058/228‑1 (MQ=255) ggcgggttaccccttttCATAAAGTTTTTGCTTTCCAGGCCGAAAATCTTGCATCGGTCCACAGGAAAGAGAAACCATGTTAAAACGTATCAAGATCGTGACCAGCTTACTGCTGGTTTTGGCCGTTTTTGGCCTTTTACAACTGACATCAGGCGGTCTGTTCTTTAATGCCTTAAAGAATGACAAAGAAAATTTCACTGTTTTACAAACCATTCGCCAGCAGCAATCCACGCTGAATGGCAGCTGGGTcg < 2:173209/236‑1 (MQ=255) tttCATAAAGTTTTTGCTTTCCAGGCCGAAAATCTTGCATCGGTCCACAGGAAAGAGAAACCATGTTAAAACGTATCAAGATCGTGACCAGCTTACTGCTGGTTTTGGCCGTTTTTGGCCTTTTACAACTGACATCAGGCGGTCTGTTCTTTAATGCCTTAAAGAATGACAAAGAAAATTTCACTGTTTTACAAACCATTCGCCAGCAGCAATCCACGCTGAATGGCAGCTGGGTCGCGTTGTTGCAGAc < 1:483538/249‑1 (MQ=255) aTAAAGATTTTGCTTTCAAGGCCGAAAATCTTGCATCGGTCCACAGGAAAGAGAAACCATGTTAAAACGTATCAAGATCGTGACCAGCTTACTGCTGGTTTTGGCCGTTTTTGGCATTTTACAACTGACATCAGGCGGTCTGTTCTTTAATGCCTTAAAGAATGACAACGAAAATTTCACTGTTTTACAAACCATTCGCCAGCAGCAATCCACGCTGAATGGCAGCTGGGTAGCGTTGTTGCAGACGCGt > 2:19553/1‑250 (MQ=255) aaaaGTTTTGGCTTTCCAGGCCGAAAAACTTGCATCGGTCCACAGGAAAGAGAAACCATGTTAAAACGTATCAAGATCGTGACCAGCTTACTGCTGGTTTTGGCCGTTTTTGGCCTTTTACAACTGACATCAGGCGGTCTGTGCGTTAATGCCTTAAAGAATGTCAAAGAAAATTGCACTGTTTTACAAACCTTTCGCCAGCAGCAATCCTCGCTGAATGGCAGCTGGGTCGCGTTGTTGCAGACGCGTaa < 2:427648/250‑1 (MQ=255) gTTTTTGCTTTCCAGGCCGAAAATCTTGCATCGGTCCACAGGAAAGAGAAACCATGTTAAAACGTATCAAGATCGTGACCAGCTTACTGCTGGTTTTGGCCGTTTTTGGCCTTTTACAACTGACATCAGGCGGTCTGTTCTTTAATGCCTTAAAGAATGACAAAGAAAATTTCACTGTTTTACAAACCATTCGCCAGCAGCAATCCACGCTGAATGGCAGCTGGGTCGCGTTGTTGCAGACGCGTAACAcc < 1:393773/251‑1 (MQ=255) gctttccAGGCCGAAAATCTTGCATCGGTCCACCGGCAAGAGAAACCATGTTAAAACGTATCAAGATCGTGACCAGCTTACTGCTGGTTTTGGCCGTTTTTGGCCTTTTACAACTGACATCAGGCGGTCTGTTCTTTAATGCCTTAAAGAATGACAAAGAAAATTTCACTGTTTTACAAACCATTCGCCAGCAGCAATCCACGCTGAATGGCAGCTGGGTCGCGTTGTTGCAGCCGCGTAACCCCCTCAAc > 2:453242/2‑251 (MQ=255) gctttccAGGCCGAAAATCTTGCATCGGTCCACAGGAAAGAGAAACCATGTTAAAACGTATCAAGATCGTGACCAGCTTACTGCTGGTTTTGGCCGTTTTTGGCCTTTTACAACTGACATCAGGCGGTCTGTTCTTTAATGCCTTAAAGAATGACAAAGAAAATTTCACTGTGTTACAAACCATTCGCCAGCAGCAATCCACGCTGAATGGCAGCTGGGTCGCGTTGTTGCAGACGCGTAACACCCTCaa < 2:148581/249‑1 (MQ=255) gCCGAAAATCTTGCATCGGTCCACAGGAAAGAGAAACCATGTTAAAACGTATCAAGATCGTGACCAGCTTACTGCTGGTTTTGGCCGTTTTTGGCCTTTTACAACTGACATCAGGCGGTCTGTTCTTTAATGCCTTAAAGAATGACAAAGAAAATTTCACTGTTTTACAAACCATTCGCCAGCAGCAATCCACGCTGAATGGCAGCTGGGTCGCGTTGTTGCAGACGCGTAACACCCTCAACCGCGCGGGt < 2:423884/251‑1 (MQ=255) tGCATCGGTCCACATGAAAGAGAAACCATGTTAAAACGTATCAAGATCGTGACCAGCTTACTGCTGGTTTTGGCCGTTTTTGGCCTTTTACAACTGACATCAGGCGGTCTGTTCTTTAATGCCTTAAAGAATGACAAAGAAAATTTCACTGTTTTACAAACCATTCGCCAGCAGCAATCCACGCTGAATGGCAGCTGGGTCGCGTTGTTGCAGACGCGTAACACCCTCAACCGCGCGGGTATCCGCTa < 1:250633/248‑1 (MQ=255) ggTCCACAGGACAGAGAGACCAGGTTAAAAAGTATAAAGCTCGTGACCAGCTTACTGCTGATTTTGTCCGTTTTTGGCCTTTTACAACTGACATCAGGCGTTCTGTTCTTTAATGCCTTAAAGAATGACAAAGAAAATATCACTGTTTTACAAACCATTAGCCAGCAGCAATCCACGATGAATGGCCGCTGGATCGCGTTGTTGAAGACGCGTCACCCCCTCAAACGCGCAGGTATcccacacatggttgg > 2:303381/1‑238 (MQ=255) caGGAAAGCGAAACCATGTTAAAACGTATCAAGATCGTGACAAGCTTAATGGTGGCTATGGCCGTTTTTGGCCTTTTACAACTGACATCAGGCGGTCTGTTCTTTAATGCCTTAAAGAATGACAAAGAAAATTTCATTGTTTTACAACACATTCGCCAGCAGCAATCCACGCTGAATGGCAGTTAGGTCGCGTTGTTGCCGACGCGTAACACCCTCAACCGCGCGGGTATCCGCTACATGATGGATCAGa > 2:248287/1‑250 (MQ=255) aaaCCATTTTAAAACGTATCAAGATCGTGACCAGTTTACTGCTGGTTTTTGCCGTTTTTGGCCTTTTACAACTGACATCAGGCGGTCTGTTCTTTAATGCCTTAAAGAATGACAAAGAAAATTTCACTGTTTTACAAACCATTCGCCAGCAGCAATCCACGCTGAATGGCAGCTGGGTCGCGTTGTTGAAGACGCGTAACACTCTCAACCGCGCGGGTTTCCGCTACATGATGGATCAGAATAATATTGGt < 2:360983/251‑1 (MQ=255) | TAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCAGGCCGAAAATCTTGCATCGGTCCACAGGAAAGAGAAACCATGTTAAAACGTATCAAAATTGTGACCAGCTTACTGCTGGTTTTGGCCGTTTTTGGCCTTTTACAACTGACATCAGGCGGTCTGTTCTTTAATGCCTTAAAGAATGACAAAGAAAATTTCACTGTTTTACAAACCATTCGCCAGCAGCAATCCACGCTGAATGGCAGCTGGGTCGCGTTGTTGCAGACGCGTAACACCCTCAACCGCGCGGGTATCCGCTACATGATGGATCAGAATAATATTGGT > NC_000913/4591486‑4591902 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |