Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,556,917	A→G	E2G (GAA→GGA)	idlP →	iraD leader peptide

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,556,917	0	A	G	95.2%	130.3 / ‑2.0	42	E2G (GAA→GGA)	idlP	iraD leader peptide
Reads supporting (aligned to +/- strand):  ref base A (1/1);  new base G (23/17);  total (24/18)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.59e-01

AATAATTTCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGGTTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTAATGAACAAAAGGGAATGGCTATGGAAAATGAGCATCAATACAGTGGTGCCCGGTGTTCAGGGCAAGCCGCATATGTTGCTAAACGTCAGGAGTGCGCAAAATGATGCGACAATCACTTCAGGCTGTTTTACCTGAAATTTCAGGCAATAAAACGTCATCGCTGCGTAAATCGGTATGCAGCGATCTCCTCACTCTTTTTAATTCTCCTCATTCGGCATTACCGTCATTACTTGTTTCAGGCATGCCCGAGTGGCAAGTG  >  NC_000913/4556675‑4557151                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                           aataatTTCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGGTTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTAATGAACAAAAGGGAATGGCTATGGGAAATGAGc                                                                                                                                                                                                                                    >  1:149082/1‑251 (MQ=255)         cccACCGGAATTAAGTAGCAGCGGTGAAAAGTATTTTCATCTACGTAACTATTCGTAATATTCAGAATATACTAGCGGTTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTCTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGTTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTAATGAACAAAAGGGAATGGCTATGGGAAATGAGCATCAATAc                                                                                                                                                                                                                            <  2:3800/251‑1 (MQ=255)            aCCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGGTTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTAATGAACAAAAGGGAATGGCTATGGGAAATGAGCATCAATACAgt                                                                                                                                                                                                                         >  1:279822/1‑251 (MQ=255)                                               aTCTACGTAACTATTCGGAATATTCAGAATATACTAGAGGTTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGAATTTTTTCCTGAAAAGTCAGTAGATATTTATAGGCAAGTAAGGTTTTATACTTTGCTGACCGGATTCAGGACTGTCTCAGAATGAAATGGATGTAATGAACAAAAGGGAATGGCTATGGGAAATGAGCATCAATACAGAGGTGCCCGGGGATCAGGGCAAGCCGCAAATGTTGc                                                                                                                                                                                      >  2:444353/1‑251 (MQ=255)                                                              cGGAATATTCAGAATATACTAGCGGTTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTAATGAACAAAAGGGAATGGCTATGGGAAATGAGCATCAATACAGTGGTGCCCGGTGTTCAGGGCAAGCCGCATATGTTGCTAAACGTCAGGAGt                                                                                                                                                                        <  1:129168/250‑1 (MQ=255)                                                                  atatTCAGAATATACTAGCGGTTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTAATGAACAAAAGGGAATGGCTATGGGAAATGAGCATCAATACAGTGGTGCCCGGTGTTCAGGGCAAGCCGCATATGTTGCTAAACGTCAGGAGTgcgc                                                                                                                                                                    >  1:187087/1‑250 (MQ=255)                                                                                            ttttGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGCCATGGATGTAATGAACAAAAGGGAATGGCTATGGAAAATGAGCATCAATACAGTGGTGCCCGGTGTTCAGGGCAAGcc                                                                                                                                                                                                 >  2:27199/1‑195 (MQ=255)                                                                                            ttttGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTAATGAACAAAAGGGAATGGCTATGGAAAATGAGCATCAATACAGTGGTGCCCGGTGTTCAGGGCAAGcc                                                                                                                                                                                                 <  1:27199/195‑1 (MQ=255)                                                                                                    ttGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTAATGAAAAAAAGGGAATGGCTATGGGAAATGAGCATCAATACAGTGGTGCCCGGTGTTCAGGGCAAGCCGCATATGTTGCTAAACGTCAGGAGTGCGCAAAATGATGCGACAATCACTTCAGGCTGTTTTAcc                                                                                                                                 >  1:200402/1‑251 (MQ=255)                                                                                                         gatTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTAATGAACAAAAGGGAACGGCTATGGGAAATGAGCATCAATACAGTGGTGCCCGGTGTTCAGGGCAAGCCGCATATGTTGCTAAACGTCAGGAGTGCGCAAAATGATGCGACAATCACTTCAGGCTGTTTTa                                                                                                                                   >  2:366860/1‑244 (MQ=255)                                                                                                         gatTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTAATGAACAAAAGGGAACGGCTATGGGAAATGAGCATCAATACAGTGGTGCCCGGTGTTCAGGGCAAGCCGCATATGTTGCTAAACGTCAGGAGTGCGCAAAATGATGCGACAATCACTTCAGGCTGTTTTa                                                                                                                                   <  1:366860/244‑1 (MQ=255)                                                                                                         gatTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTAACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTAATGAACAAAAGGGAATGGCTATGGGAAATGAGCATCAATACAGTGGTGCCCGGTGTTCAGGGCAAGCCGCATATGTTGCTAAACGTCAGGAGTGCGCAAAATGATGCGACAATCACTTCAGGCTGTTTTACCTGaaa                                                                                                                            <  1:129116/251‑1 (MQ=255)                                                                                                               tttCAAATCATGGATTTTTTCCGGAAAAGTAAGTAGATATTAATAGGCAAGTAAGGTTTGATCCTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTAATGAACAAAAGGGAATGGCTATGGGAAATGATCATCAATACAGGGGTGCCCGGTGTGCAGGGCAAGCCGCATATGTTGCTAAACGTCAGGAGTGCGCAAAAGGATGCGAAAATCACTTCAGGATGTTTTGCCTGAAATCTCa                                                                                                                       <  2:332737/248‑1 (MQ=255)                                                                                                               tATCGAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTAATGAACAAAAGGGAATGGCTATGGGAAATGAGCATCAATACAGTGGTGCCCGGTGTTCAGGGCAAGCCGCATATGTTGCTAAACGTCAGGAGTGCGCAAAATGATGCGACAATCACTTCAg                                                                                                                                            <  2:13280/229‑1 (MQ=255)                                                                                                               tATCGAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTAATGAACAAAAGGGAATGGCTATGGGAAATGAGCATCAATACAGTGGTGCCCGGTGTTCAGGGCAAGCCGCATATGTTGCTAAACGTCAGGAGTGCGCAAAATGATGCGACAATCACTTCAg                                                                                                                                            >  1:13280/1‑229 (MQ=255)                                                                                                                 tCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGCTTCAGGCCTGTCTCAGACTGACATGGATGTAATGAACAAAAGGGAATGGCTATGGGAAATGAGCATCAATACAGTGGTGCCCGGTGTTCAGGGCAAGCCGCATATGTTGCTAAACGTCAGGAGTGCGCAAAATGATGCGCCAATCACTTCAGGCTGTTTTACCTGAAATTTCAGGc                                                                                                                    >  2:293891/1‑251 (MQ=255)                                                                                                                     aTCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTAATGAACAAAAGGGAATGGCTATGGGAAATGAGCATCAATACAGTGGTGCCCGGTGTTCAGGGCAAGCCGCATATGTTGCTAAACGTCAGGAGTGCGCAAAATGATGCGACAATCACTTCAGGCTGTTTTACCTGAAATTTCAGGCAAt                                                                                                                 >  1:225340/1‑250 (MQ=255)                                                                                                                          gACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTAATGAACAAAAGGGAATGGCTATGGGAAATGAGCATCAATACAGTGGTGCCCGGTGTTCAGGGCAAGCCGCATATGTTGCTAAACGTCAGGAGTGCGCAAAATGATGCGACAATCACTTCAGGCTGTTTTACCTGAAATTTCAGGCAATAAAAc                                                                                                            <  1:363849/250‑1 (MQ=255)                                                                                                                          gACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTAATGAACAAAAGGGAATGGCTATGGGAAATGAGCATCAATACAGTGATGCCCGGTGTTCAGGGCAAGCCGCATATGTTGCTAAACGTCAGGAGTGCGCAAAATGATGCGACAATCACTTCAGGCTGTTTTACCTGAAATTTCAGGCAATAAAACg                                                                                                           >  2:434371/1‑251 (MQ=255)                                                                                                                                 ttCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTAATGAACAAAAGGGAATGGCTATGGGAAATGAGCATCAATACAGTGGTGCCCGGTGTTCAGGGCAAGCCGCATATGTTGCTAAACGTCAGGAGTGCGCAAAATGATGCGACAATCACTTCAGGCTGTTTTACCTGAAATTTCAGGCAATAAAACGTCATCg                                                                                                     <  1:293891/250‑1 (MQ=255)                                                                                                                                          aGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTAATGAACAAAAGGGAATGGCTATGGGAAATGAGCATCAATACAGTGGTGCCCGGTGTTCAGGGCAAGCCGCATATGTTGCTAAACGTCAGGAGTGCGCAAAATGATGCGACAATCACTTCAGGCTGTTTTACCTGAAATTTCAGGCAATAAAACGTCATCGCTGCGTAAAt                                                                                           <  1:5506/251‑1 (MQ=255)                                                                                                                                           gTCAGTAGATATTCATAGGCAAGTAAGATTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTAATGAACAAAAGGGAATGGCTATGGGAAATGAGCATCAATACAGTGTTGCCCGGTGTTCAGGGCAAGCCGCATATGTTGCTAAACGTCAGGAGTGCGCAAAATGATGCGACAATCACTTCAGGCTGTTTTACCTGAAATTTCAGGCAATAAAACGTCATCGCTGCGTAAATc                                                                                          <  2:324761/251‑1 (MQ=255)                                                                                                                                                       tCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTAATGAACAAAAGGGAATGGCTATGGGAAATGAGCATCAATACAGTGGTGCCCGGTGTTCAGGGCAAGCCGCATATGTTGCTAAACGTCAGGAGTGCGCAAAATGATGCGACAATCACTTCAGGCTGTTTTACCTGAAATTTCAGGCAATAAAACGTCATCGCTGCGTAAATCGGTATGCa                                                                                  <  2:93873/247‑1 (MQ=255)                                                                                                                                                       tCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTAATGAACAAAAGGGAATGGCTATGGGAAATGAGCATCAATACAGTGGTGCCCGGTGTTCAGGGCAAGCCGCATATGTTGCTAAACGTCAGGAGTGCGCAAAATGATGCGACAATCACTTCAGGCTGTTTTACCTGAAATTTCAGGCAATAAAACGTCATCGCTGCGTAAATCGGTATGCa                                                                                  >  1:93873/1‑247 (MQ=255)                                                                                                                                                                            taCTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTAATGAACAAAAGGGAATGGCTATGGGAAATGAGCATCAATACAGTGGTGCCCGGTGTTCAGGGCAAGCCGCATATGTTGCTAAACGTCAGGAGTGCGCAAAATGATGCGACAATCACTTCAGGCTGTTTTACCTGAAATTTCAGGCAATAAAACGTCATCGCTGCGTAAATCGGTATGCAGCGATCTCCTCACTCTTTTTAATtc                                                         >  1:202511/1‑251 (MQ=255)                                                                                                                                                                             aCTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTAATGAACAAAAGGGAATGGCTATGGGAAATGAGCATCAATACAGTGGTGCCCGGTGTTCAGGGCAAGCCGCATATGTTGCTAAACGTCAGGAGTGCGCAAAATGATGCGACAATCACTTCAGGCTGTTTTACCTGAAATTTCAGGCAATAAAACGTCATCGCTGCGTAAATCGGTATGCAGCGATCTCCTCACTCTTTTTAATtct                                                        >  1:382109/1‑251 (MQ=255)                                                                                                                                                                                    tGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTAATGAACAAAAGGGAATGGCTATGGGAAATGAGCATCAATACAGTGGTGCCCGGTGTTCAGGGCAAGCCGCATATGTTGCTAAACGTCAGGAGTGCGCAAAATGATGCGACAATCACTTCAGGCTGTTTTACCTGAAATTTCAGGCAATAAAACGTCATCGCTGCGTAAATCGGTATGCAGCGATCTCCTCACTCTTTTTATTTCTCCTCAtt                                                 >  2:75453/1‑251 (MQ=255)                                                                                                                                                                                    tGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTAATGAACAAAAGGGAATGGCTATGGGAAATGAGCATCAATACAGTGGTGCCCGGTGTTCAGGGCAAGCCGCATATGTTGCTAAACGTCAGGAGTGCGCAAAATGATGCGACAATCACTTCAGGCTGTTTTACCTGAAATTTCAGGCAATAAAACGTCATCGCTGCGTAAATCGGTATGCAGCGATCTCCTCACTCTTTTTAATTCTCCTCAtt                                                 >  1:58338/1‑251 (MQ=255)                                                                                                                                                                                      aCAGGATTCAGGCCTGTCGAAGACTGACATGGATGTAATGAACAAAAGGGAATGGCTATGGGAAATGAGCATCAATACAGTGGTGCCCGGTGTTCAGGGCAAGCCGCATATGTTGCTAAACGTCAGGAGTGCGCAAAATGATGCGACAATCACTTCAGGCTGTTTTACCTGAAATTTCAGGCAATAAAACGTCATCGCTGCGTAAATCGGTATGCAGCGATCTCCTCACTCTTTTTAATTCTCCTCATTCg                                               <  2:263825/251‑1 (MQ=255)                                                                                                                                                                                              cAGGCCTGTCTCAGACTGACATGGATGTAATGAACAAAAGGGAATGGCTATGGGAAATGAGCATCAATACAGTGGTGCCCGGTGTTCAGGGCAAGCCGCATATGTTGCTAAACGTCAGGAGTgcg                                                                                                                                                                     >  1:160704/1‑125 (MQ=255)                                                                                                                                                                                              cAGGCCTGTCTCAGACTGACATGGATGTAATGAACAAAAGGGAATGGCTATGGGAAATGAGCATCAATACAGTGGTGCCCGGTGTTCAGGGCAAGCCGCATATGTTGCTAAACGTCAGGAGTgcg                                                                                                                                                                     <  2:160704/125‑1 (MQ=255)                                                                                                                                                                                                ggCCTGTCTCAGACTGACATGGATGTAATGAACAAAAGGGAATGGCTATGGGAAATGAGCATCAATACAGTGGTGCCCGGTGTTCAGGGCAAGCCGCATATGTTGCTAAACGTCAGGAGTGCGCAAAATGATGCGACAATCACTTCAGGCTGTTTTACCTGAAATTTCAGGCAATaaa                                                                                                              <  1:50356/178‑1 (MQ=255)                                                                                                                                                                                                ggCCTGTCTCAGACTGACATGGATGTAATGAACAAAAGGGAATGGCTATGGGAAATGAGCATCAATACAGTGGTGCCCGGTGTTCAGGGCAAGCCGCATATGTTGCTAAACGTCAGGAGTGCGCAAAATGATGCGACAATCACTTCAGGCTGTTTTACCTGAAATTTCAGGCAATaaa                                                                                                              >  2:50356/1‑178 (MQ=255)                                                                                                                                                                                                   cTGTCTCAGACTGACATGGATGTAATGAACAAAAGGGAATGGCTATGGGAAATGAGCATCAATACAGTGGTGCCCGGTGTTCAGGGCAAGCCGCATATGTTGCTAAACGTCAGGAGTGCGCAAAATGATGCGACAATCACTTCAGGCTGTTTTACCTGAAATTTCAGGCAATAAAACGTCATCGCTGCGTAAATCGGTATGCAGCGATCTCCTCACTCTTTTTAATTCTCCTCATTCGGCATTACCGTCAt                                  >  2:155914/1‑251 (MQ=255)                                                                                                                                                                                                               gACATGGATGTAATGAACAAAAGGGAATGGCTATGGGAAATGAGCATCAATACAGTGGTGCCCGGTGTTCAGGGCAAGCCGCATATGTTGCTAAACGTCAGGAGTGCGCAAAATGATGCGACAATCACTTCAGGCTGTTTTACCTGAAATTTCAGGCAATAAAACGTCATCGCTGCGTAAATCGGTATGCAGCGATCTCCTCACTCTTTTTAATTCTCCTCATTCGGCATTACCGTCATTACTTGTTTCa                       <  1:444353/250‑1 (MQ=255)                                                                                                                                                                                                                aCATGGATGTAATGAACAAAAGGGAATGGCTATGGGAAATGAGCATCAATACAGTGGTGCCCGGTGTTCAGGGCAAGCCGCATATGTTGCTAAACGTCAGGAGTGCGCAAAATGATGCGACAATCACTTCAGGCTGTTTTACCTGAAATTTCAGGCAATAAAACGTCATCGCTGCGTAAATCGGTATGCAGCGATCTCCTCACTCTTTTTAATTCTCCTCATTCGGCATTACCGTCATTACTTGTTTCAgg                     >  1:475902/1‑251 (MQ=255)                                                                                                                                                                                                                       tGTAATGAACAAAAGGGAATGGCTATGGGAAATGAGCATCAATACAGTGGTGCCCGGTGTTCAGGGCAAGCCGCATATGTTGCTAAACGTCAGGAGTGCGCAAAATGATGCGACAATCACTTCAGGCTGTTTTACCTGAAATTTCAGGCAATAAAACGTCATCGCTGCGTAAATCGGTATGCAGCGATCTCCTCACTCTTTTTAATTctcctc                                                    <  1:416598/213‑1 (MQ=255)                                                                                                                                                                                                                       tGTAATGAACAAAAGGGAATGGCTATGGGAAATGAGCATCAATACAGTGGTGCCCGGTGTTCAGGGCAAGCCGCATATGTTGCTAAACGTCAGGAGTGCGCAAAATGATGCGACAATCACTTCAGGCTGTTTTACCTGAAATTTCAGGCAATAAAACGTCATCGCTGCGTAAATCGGTATGCAGCGATCTCCTCACTCTTTTTAATTctcctc                                                    >  2:416598/1‑213 (MQ=255)                                                                                                                                                                                                                                  aaaGGGAATGGCTATGGGAAATGAGCATCAATAAAGTGGTGCCCGGTGTTCAGGGCAAGCCGCATATGTTGCTAAACGTCAGGAGTGCGCAAAATGATGCGACAATCACTTCAGGCTGTTTTACCTGAAATTTCAGGCAATAAAACGTCATCGCTGCGTAAATCGGTATGCAGCGATCTCCTCACTCTTTTTAATTCTCCTCATTCGGCATTACCGTCATTACTTGTTTCAGGCATGCCCGAGTGGCAAGt   >  2:279133/1‑251 (MQ=255)                                                                                                                                                                                                                                   caGGGAATGGCTATGGGGAATGAGCATCAAGACATTGGTGCCAGGTGTGAAGGGCAAGGCGCATATGTTGCTAAACGTCAAGCGTGCGCAACATGATGCGACAATCACTTCAGGCTGTTTTACCTGAAATTTCAGGCACTCAAGCGTCATCGCTGCGGAAATCGGTATGCAGCGATCTCCTCACTCTTTTTAATTATCCTCATTAGGCATTACAGTCATTCATTGTTTCAggactgcctgcttggcaagtg  >  2:109165/2‑232 (MQ=255)                                                                                                                                                                                                                                             cTATGGGAAATGAGCATCAATACAGTGGTGCCCGGTGTTCAGGGCAAGCCGCATATGTTGCTAAACGTCAGGAGTGCGCAAAATGATGCGACAATCACTTCAGGCTGTTTTACCTGAAATTTCAGGCAATAAAACGTCATCGCTGCGTAAATCGGTATGCAGCGATCTCCTCACTCTTTTTAATTCTCCTCATTCGGCa                                            >  2:407117/1‑199 (MQ=255)                                                                                                                                                                                                                                             cTATGGGAAATGAGCATCAATACAGTGGTGCCCGGTGTTCAGGGCAAGCCGCATATGTTGCTAAACGTCAGGAGTGCGCAAAATGATGCGACAATCACTTCAGGCTGTTTTACCTGAAATTTCAGGCAATAAAACGTCATCGCTGCGTAAATCGGTAGGCAGCGATCTCCTCACTCTTTTTAATTCTCCTCATTCGGCa                                            <  1:407117/199‑1 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                           AATAATTTCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGGTTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTAATGAACAAAAGGGAATGGCTATGGAAAATGAGCATCAATACAGTGGTGCCCGGTGTTCAGGGCAAGCCGCATATGTTGCTAAACGTCAGGAGTGCGCAAAATGATGCGACAATCACTTCAGGCTGTTTTACCTGAAATTTCAGGCAATAAAACGTCATCGCTGCGTAAATCGGTATGCAGCGATCTCCTCACTCTTTTTAATTCTCCTCATTCGGCATTACCGTCATTACTTGTTTCAGGCATGCCCGAGTGGCAAGTG  >  NC_000913/4556675‑4557151

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑