Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,336	A→G	E201E (GAA→GAG)	thrC →	threonine synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,336	0	A	G	96.5%	172.0 / ‑2.6	58	E201E (GAA→GAG)	thrC	threonine synthase
Reads supporting (aligned to +/- strand):  ref base A (1/1);  new base G (29/27);  total (30/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.30e-01

ACCCATATTGCGGGTGATAAGCCAGTGACCATTCTGACCGCGACCTCCGGTGATACCGGAGCGGCAGTGGCTCATGCTTTCTACGGTTTACCGAATGTGAAAGTGGTTATCCTCTATCCACGAGGCAAAATCAGTCCACTGCAAGAAAAACTGTTCTGTACATTGGGCGGCAATATCGAAACTGTTGCCATCGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGAACTGAAAGTGGCGCTAGGGTTAAACTCGGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCACTCGGTCTGCC‑GGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCC  >  NC_000913/4088‑4580                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                      aCCCATATTGCGGGTGATAAGCCAGTGACCATTCTGACCGCGACCTCCGGTGATACCGGAGCGGCAGTGGCTCATGCTTTCTACGGTTTACCGAATGTGAAAGTGGTTATCCTCTATCCACGAGGCAAACTCAGTCCACTGCAAGAAAAACTGTTCTGTACATTGGGCGGCAATATCGAAAATGTTGCCATCGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGGTGAAGAACt                                                                                                                                                                                                                                                     >  2:222595/1‑251 (MQ=255)     atatTGCGGGCGATAAGCCAGTGACCATTCTGACCGCGACCTCCGGTGATACCGGAGCGGCAGTGGCTCATGCTTTCTACGGTTTACCGAATGTGAAAGTGGTTATCCTCTATCCACGAGGCAAAATCAGTCCACTGCAAGAAAAACTGTTCTGTACATTGGGCGGCAATATCGCAACTGTTGCCATCGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCTGTTGATGATGAAGAGCTGaa                                                                                                                                                                                                                                                  >  2:378608/1‑250 (MQ=255)             ggcgATAAGCCAGTGACCATTCGGACCGCGACCTCCGGTGATACCGGAGCGGCAGGGGCTCATGCTTTCTACGGTTTACCGAGTGTGAAAGGGGTTAACCTCTATCCACGGGGCCAAATCAGGCCACTGCAAGAAAAACTGTTCTGTACATTGGGCGGCAAAATCGAAACTGTTGCAATGGACGTCGATTTGGTTGACAGGCAGGCGCTGGTGAAGAGGGCGTTTGATGATGTAGGGCTGCAAGTGgcg                                                                                                                                                                                                                                           >  2:314330/4‑249 (MQ=255)                gATAAGCCAGTGACCATTCTGACCGCGACCTCCGGTGATACCGGAGCGGCAGTGGCTCATGCTTTCTACGGTTTACCGAATGTGAAAGTGGTTATCCTCTATCCACGAGGCAAAATCAGTCCACTGCAAGAAAAACTGTTCTGTACATTGGGCGGCAATATCGAAACTGTTGCCATCGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGTTGAAGAGCTGAAAGTGgc                                                                                                                                                                                                                                            >  2:424013/1‑245 (MQ=255)                gATAAGCCAGTGACCATTCTGACCGCGACCTCCGGTGATACCGGAGCGGCAGTGGCTCATGCTTTCTACGGTTTACCGAATGTGAAAGTGGTTATCCTCTATCCACGAGGCAAAATCAGTCCACTGCAAGAAAAACTGTTCTGTACATTGGGCGGCAATATCGAAACTGTTGCCATCGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGAGCTGAAAGTGgc                                                                                                                                                                                                                                            <  1:424013/245‑1 (MQ=255)                 aTAAGCCAGTGACCATTCTGACCGCGACCTCCGGTGATACCGGAGCGGCAGTGGCTCATGCTTTCTACGGTTTACCGAATGTGAAAGTGGTTATCCTCTATCCACGAGGCAAAATCAGTCCACTGCAAGAAAAACTGTTCTGTACATTGGGCGGCAATATCGAAACTGTTGCCATCGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGAGCTGAAAGTGGCGCTgggg                                                                                                                                                                                                                                     <  2:225938/251‑1 (MQ=255)                   aaGCCAGTGACCATTCTGACCGCGACCTCCGGTGATACCGGAGCGGCAGTGGCTCATGCTTTCTACGGTTTACCGAATGTGAAAGTGGTTATCCTCTATCCACGAGGCAAAATCAGTCCACTGCAAGAAAAACTGTTCTGTACATTGGGCGGCAATATCGAAACTGTTGCCATCGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGACGCAGGCGTTTGATGATGAAGAGCTGAAAGTGGCGCTGGGGtt                                                                                                                                                                                                                                   >  1:353567/1‑251 (MQ=255)                    aGCCAGTGACCATTCTTACCGCGACCTCCGGTGATTCCGGAGCTGCAGTGGCTCATGCTTTCTGCGGGTTACCGAATGTGAAAGTGGTTATCCTCTATCCACGAGGCAAAATCAGTCCACTGCAAGAAAAACTGTTCTGTACATTGGGCGGCAATATCGAAACTGTTGCCAGCGACGGCGATTTCGATGCCTGTCAGGCGCTGGGGAAGCAGGCGTTTGATGATGAAGAGCTGAAAGTGGCGCTGGGGTTa                                                                                                                                                                                                                                  <  2:69892/251‑1 (MQ=255)                    aGCCAGTGACCATTCTGACCGCGACCTCCGGTGATACCGGAGCGGCAGTGGCTCATGCTTTCTACGGTTTACCGAATGTGAAAGTGGTTATCCTCTATCCACGAGGCAAAATCAGTCCACTGCAAGAAAAACTGTTCTGTACATTGGGCGGCAATATCGAAACTGTTGCCATCGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGAGCTGAAAGTGGCGCTGGGGTTa                                                                                                                                                                                                                                  <  2:28287/251‑1 (MQ=255)                           gACCATTCTGACCGCGACCTCCGGTGATACCGGAGCGGCAGTGGCTCATGCTTTCTACGGTTTACCGAATGTGAAAGTGGTTATCCTCTATCCCCGAGGCAAAATCAGTCCACTGCAAGAAACACTGTTCTGTACATTGGGCGGCAATATCGAAAATGTTGCCATCGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGCAGAGCTGAACGTGGCGCTGGGGTTAAACTc                                                                                                                                                                                                                             >  2:234080/1‑249 (MQ=255)                             ccATTCTGACCGCGACCTCCGGTGATACCGGAGCGGCAGTGGCTCATGCTTTCTACGGTTTACCGAATGTGAAAGTGGTTATCCTCTATCCACGAGGCAAAATCAGGCCACTGCAAGAAAAACTGTTCTGTACATTGGGCGGCAATATCGAAACTGTTGCCATCGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGAGCTGAAAGTGGCGCTGGGGTTAAACTCAGCt                                                                                                                                                                                                                         <  1:338859/251‑1 (MQ=255)                              catgcTGACCGCGACCTGCGGTGATACCGGAGCGGCAGTGGCGCATGTTTTCTACGGTTTACCTAATGTGAAAGGGATTATCGGCTATCCACGAGGCTAAATCAGTCCTCTGCTAGAAAAACTGTTCTGTACATTGGGCGGCAATATCGAAACTGTTGCTATCGCCGGCAATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTGTGATGATGAAGAGCTGAAAGTGGCGCTGGGGTTATACTCAGCTa                                                                                                                                                                                                                        <  2:244474/247‑1 (MQ=255)                              cATTCTGACCGCTACCTCCGGTGATACCGGAGCGGCAGTGGCTCATGCTTTCTACGGTTTACCGAATGTGAAAGTGGTTATCCTCTATCCACGAGGCAAAATCAGTCCACTGCAAGAAAAACTGTTCTGTACATTGGGCGGCAATATCGAAACTGTTGCCATCGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGAGCTGAAAGTGGCGCTGGGGTTAAACTCAGCt                                                                                                                                                                                                                         >  1:229784/1‑250 (MQ=255)                                 tCTGACCGCGACCTCCGGTGATACCGGAGCGGCAGTGGCTCATGCTTTCTACGGTTTACCGAATGTGAAAGTGGTTATCCTCTATCCACGAGGCAAAATCAGTCCACTGCAAGAAAAACTGTTCTGTACATTGGGCGGCAATATCGAAACTGTTGCCATCGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGAGCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACt                                                                                                                                                                                                                     >  2:314494/1‑251 (MQ=255)                                    gACCGCGACTGCCGGTGATTCCGGCGCGGCAGTGGCTCATGTTGTCTACGGTTTACCGAATGTGAAAGTGGTTATCCTCTATCCACGAGGCAAAATCAGTCCACTGCAAGAAAAACTGTTCTGTACATTGGGCGGCAATATCGAACCTGTTGCCATCGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGAGCTGAAATTGGCGCTGGGGTTAAACTCAGCTAACTCg                                                                                                                                                                                                                   <  1:190854/250‑1 (MQ=255)                                       cgcgACCTCCGGTGGTACCGGTGCGGCAGTGGCTCAGGCTTTCTACGTGTTACCGAATGTGAAAGTGGTTATCCTCTATCCACGAGGCAAAAACAGGCCACTGCAATAAAAACTGTTCTGTACATTGGGCGGCAATATCGAAACTGGTGCCATCGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGAGCTGAAAGTGGCGCTGGGGTTAACCTCAGCTATCTCGATTa                                                                                                                                                                                                               <  2:16663/251‑1 (MQ=255)                                            ccTCCGGTGATACCGGAGCGGCAGTGGCTCATGCTTTCTACGGTTTACCGAATGTGAAAGTGGTTATCCTCTATCCACGAGGCAAAATCAGTCCACTGCAAGAAAAACTGTTCTGTACATTGGGCGGCAATATCGAAACTGTTGCCATCGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGAGCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATc                                                                                                                                                                                                          <  1:49830/251‑1 (MQ=255)                                                    gATACCTGAGCGGCAGTGGCTCATGCTTTCTACGTTTTAACGAATGAGAAAGGGGTTATCCTCTATCCACGAGGCAAAATCAGTCCAATGCAAGAAAAACTGTTCTGTACATTGGGCGGCAATATCGAAACTGTTGCCATCGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGAGCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGCTTAACATCAGCCGttt                                                                                                                                                                                                  <  1:229863/251‑1 (MQ=255)                                                       aCCGGAGCGGCAGTGGCTCATGCTTTCTACGGTTTACCGAATGTGAAAGTGGTTATCCTCTATCCACGAGGCAAAATCAGTCCACTGCAAGAAAAACTGTTCTGTACATTGGGCGGCAATATCGAAACTGTTGCCATCGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGAACTGAAAGTGGCGCTAGGGTTAAACTCGGCTAACTCGATTAACATCAGCCGTTTGc                                                                                                                                                                                                <  1:190582/250‑1 (MQ=255)                                                        ccGGAGCGGCAGTGGTTCATGCTTTCTACTGTTTACCGAATGTGAAAGGGGTTATCCTCTATCCACGAGGCAAAATCAGTCCACTGCAAGAAAAACTGTTCTGTACATTGGGCGGCAATAGTGAGACTGTTGCCATCGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGAGCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAGCCGTTTGCTg                                                                                                                                                                                              <  1:437941/251‑1 (MQ=255)                                                              cggcatcggcgtaggCTTTCTACGGGTTTGCGAATGTGAAAGTGGTTATCTCCTAGCCACGAGGCAAAAGCAGTCCACTGCAAGAAAAACTGTTCTGTACATTGGGCGGCAATATCGAAACTTTTGCCATCGAAGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCTTTTGAGGATGAAGAGCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAg                                                                                                                                                                                        <  2:261279/237‑1 (MQ=255)                                                                 cAGTGGCTCATGCTTTCTACGGTTTACCGAATGTGAAAGTGGTTATCCTCTATCCACGCGGAAAAATCAGTCCAATGCAAGAAAAACTGTTCTGTACATTGGGCGGCAATATCGAAACTGTTGCCATCGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGAGCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGAtt                                                                                                                                                                                     >  1:311021/1‑251 (MQ=255)                                                                     ggCTCATGCTTTCTACGGTTTACCGAATGTGAAAGTGGTTATCCTCTATCCACGAGGCAAAATCAGTCCACTGCAAGAAAAACTGTTCTGTACATTGGGCGGCAATATCGAAACTGTTGCCATCGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGAGCTGAAAGTGgc                                                                                                                                                                                                                                            >  1:108842/1‑192 (MQ=255)                                                                     ggCTCATGCTTTCTACGGTTTACCGAATGTGAAAGTGGTTATCCTCTATCCACGAGGCAAAATCAGTCCACTGCAAGAAAAACTGTTCTGTACATTGGGCGGCAATATCGAAACTGTTGCCATCGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGAGCTGAAAGTGgc                                                                                                                                                                                                                                            <  2:108842/192‑1 (MQ=255)                                                                          aTGCTTTCTACGGTTTACCGAATGTGAAAGTGGTTATCCTCTATCCACGAGGCAAAATCAGTCCACTGCAAGAAAAACTGTTCTGTACATTGGGCGGCAATATCGAAACTGTTGCCATCGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGAGCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCtactac                                                                                                                                                                            <  2:272945/251‑1 (MQ=255)                                                                               ttCTACGGTTTACCGAATGTGAAAGTGGTTATCCTCTATCCACGAGGCAAAATCAGTCCACTGCAAGGAAAACTGTTCTGTACATTGGGCGGCAATATCGAAACTGTTGCCATCGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGAGCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTg                                                                                                                                                                        >  1:263927/1‑250 (MQ=255)                                                                                  tACGGTTTACCGAATGTGAAAGTGGTTATCCTCTATCCACGAGGCAAAATCAGTCCACTGCAAGAAAAACTGTTCTGTACATTGGGCGGCAATATCGAAACTGTTGCCATCGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGAGCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGc                                                                                                                                                                    >  1:271782/1‑251 (MQ=255)                                                                                     ggTTTACCGAATGTGAAAGTGGTTATCCTCTATCCACGAGGCAAAATCAGTCAACTGCAAGAAAAACTGTTCTGTACATTGGGCGGCAATATCGAAACTGTTGCCATCGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGAGCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGcagt                                                                                                                                                                 >  1:291551/1‑248 (MQ=255)                                                                                      gTTTACCGAATGTGAAAGTGTTTATCCTCTATCCACGAGGCAAAATCAGTCCACTGCAAGAAAAACTGTTCTGTACATTGGGCGGCAATATCGAAACTGTTGCCATCGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGAGCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCAGtt                                                                                                                                                                <  1:372504/251‑1 (MQ=255)                                                                                      gTTTACCGAATGTGAAAGTGGTTATCCTCTATCCACGAGGCAAAATCAGTCCACTGCAAGAAAAACTGTTCTGTACATTGGGCGGCAATATCGAAACTGTTGCCATCGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGACGAGCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCCGATTTGCTACTACTTTGCAGCAGtt                                                                                                                                                                >  2:82443/1‑251 (MQ=255)                                                                                      gTTTACCGAATGTGAAAGTGGTTATCCTCTATCCACGAGGCAAAATCAGTCCACTGCAAGAAAAACTGTTCTGTACATTGGGCGGCAATATCGAAACTGTTGCCATCGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGAGCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCAGtt                                                                                                                                                                >  1:165690/1‑251 (MQ=255)                                                                                         tACCGAATGTGAAAGTGGTTATCCTCTATCCACGAGGCAAAATCAGTCCACTGCAAGAAAAACTGTTCTGTACATTGGGCGGCAATATCGAAACTGTTGCCATCGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGAGCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCAGTTgcg                                                                                                                                                             >  2:416985/1‑251 (MQ=255)                                                                                         tACCGAATGTGAAAGTGGTTATCCTCTATCCACGAGGCAAAATCAGTCCACTGCAAGAAAAACTGTTCTGTACATTGGGCGGCAATATCGAAACTGTTGCCATCGACGGCGATTTCGAAGCCTGTCAGGCGCTGGTGAAGAAGGAGTTTGATGATGAAGAGCTGAAAGTGGCGCTGGGGTAAAACTCAGCGAACTCGATTAAAATCAGCCGTTTGCTGGCGCAGATTTGATACTACTTTGAAGCAGTTgcg                                                                                                                                                             >  2:385582/1‑251 (MQ=255)                                                                                             gAATGTGAAAGTGGTTATCCTCTATCCACGAGGCAAAATCAGTCCACTGCAAGAAAAACTGTTCTGTACATTGGGCGGCAATATCGAAACTGTTGCCATCGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGAGCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCAGTTGCGCAGc                                                                                                                                                         >  1:87499/1‑251 (MQ=255)                                                                                                tgtgAAAGTGGTTATCCTCTATCCACGAGGCAAAATCAGTCCACTGCAAGAAAAACTGTTCTGTACATTGGGCGGCAATATCGAAACTGTTGCCATCGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGGTTGATGATGAAGAGCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCAGTTGCGCAGCTGc                                                                                                                                                      >  1:25999/1‑251 (MQ=255)                                                                                                               ccTCTATCCACGAGGCAAAATCAGTCCACTGCAAGAAAAACTGTTCTGTACATTGGGCGGCAATATCGAAACTGTTGCCATCGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGAGCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCAGTTGCGCAGCTGCCGCAGgaagcgcgac                                                                                                                                       >  2:315981/1‑243 (MQ=255)                                                                                                                    aTCCACGAAGCAAAATCAGTCCACTGCAAGAAAAACTGTTCTGTACATTGGGCGGCAATATCGAAACTGTTGCCATCGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGAGCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCAGTTGCGCAGCTGCCGCAGGAAGCGCGCAACCAg                                                                                                                                  <  1:287994/251‑1 (MQ=255)                                                                                                                        aCGAGGCAAAATCAGTCCACTGCAAGAAAAACTGTTCTGTACATTGGGCGGCAATATCGAAACTGTTGCCATCGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGAGCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCAGTTGCGCAGCTGCCGCAGGAAGCGCGCAACCAGCTgg                                                                                                                              >  2:478783/1‑251 (MQ=255)                                                                                                                              cAAAATCAGTCCACTGCAAGAAAAACTGTTCTGTACATTGGGCGGCAATATCGAAACTGTTGCCATCGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGAGCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCAGTTGCGCAGCTGCCGCAGGAAGCGCGCAACCAGCTGGTTGtct                                                                                                                        <  1:314494/251‑1 (MQ=255)                                                                                                                                             gCAAGAAAAACTGTTCTGTACATTGGGCGGCAATATCGAAACTGTGGCCATCGACGGCGATTTCGAGGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGAGCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCAGTTGCGCAGCTGCCGCAGGAAGCGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGaa                                                                                                         <  2:441470/251‑1 (MQ=255)                                                                                                                                                              gTACATTGGGCGGCAATATCGAAACTGTTGCCATCGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGAGCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCAGTTGCGCAGCTGCCGCAGGAAGCGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGAc                                                                                         <  1:378608/250‑1 (MQ=255)                                                                                                                                                               tACATTGGGCGGCAATATCGAAACTGTTGCCATCGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGAGCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCAGTTGCGCAGCTGCCGCAGGAAGCGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGAcgg                                                                                       >  2:40017/1‑251 (MQ=255)                                                                                                                                                                       gcgacAATATCGAAACTGTTGCCATCGACGGCGATTTCGATGGCTGGCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGAGCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAGTCGTTTGCTGGCGCAGATTTGCTACTCCTTTGAAGCAGTTGCGCAGCTGCCGCAGGAAGCGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGAAACCTTCGGCGATTTGAcggcggtgtgt                                                                               >  2:110777/5‑246 (MQ=255)                                                                                                                                                                        cggcAATATCGAAACTGTTGCCATCGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGAGCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCAGTTGCGCAGCTGCCGCAGGAAGCGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGGctgc                                                                              >  1:175353/1‑251 (MQ=255)                                                                                                                                                                            aaTATCGAAACTGTTGCCATCGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGAGCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAGCTGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCAGTTGCGCAGCTGCCGCAGGAAGCGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGc                                                                          <  1:174879/251‑1 (MQ=255)                                                                                                                                                                               atCGAAACTGTTGCCATCGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGAGCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCAGTTGCGCAGCTGCCGCAGGAAGCGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGaa                                                                       <  1:315981/251‑1 (MQ=255)                                                                                                                                                                                    aaCTGTTGCCATCGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGAGCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCAGTTGCGCAGCTGCCGCAGGAAGCGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCggggcgtctgggggagggac                                                                  >  2:424058/1‑234 (MQ=255)                                                                                                                                                                                        ggtGCCAGCGACGGCGATTTCGATGCCTGGCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGAGCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAGCCGTTTGCGGGCGCAGATTTGCTACTACTTTGAAGCAGTTGCGCAGCTGCCGCAGGAAGCGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCACTCgg                                                              <  2:263927/249‑1 (MQ=255)                                                                                                                                                                                         ttGCAATCGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTAGATGATGAAGAGCTGAAAGTGACGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCCGATTTGCTACTACTTTGAAGCCGTTGCGCAGCTGCCTCAGGAAGCGCGCAACCAGCTGGTAGTCTCGGTGCCAGGCAGACACTTCAGCGCTTTGCCGGCgggattatcgaccaagacaaccgcc                                                             >  2:297593/1‑229 (MQ=255)                                                                                                                                                                                                 gACGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGAGCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCAGTTGCGCAGCTGCCGCAGGAAGCGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCAccccggctgcccgg                                                     >  1:394372/1‑238 (MQ=255)                                                                                                                                                                                                  aCGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGAGCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCAGTTGCGCAGCTGCCGCAGGAAGCGCGCAACCAGCTGGTTGTCTCGGTGCCGAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGCAGGCACTCGGTCTGCC‑gggg                                                   >  1:125055/1‑249 (MQ=255)                                                                                                                                                                                                       gATTACGATGCCTGTCAGGCGCTGGTGAAGCAGGGGTTTGATGATGAAGAGCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACGACTTTGAAGCAGTTGCGCAGCTGCCGCAGGAAGCGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCACGCGGTCTGCC‑GGTGAAACg                                              <  2:67633/251‑1 (MQ=255)                                                                                                                                                                                                                      cAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGAGCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCAGTTGCGCAGCTGCCGCAGGAAGCGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGGTGGGGGAGTCACCCGGTCTGCC‑gggggaacggtttttttgttcgac                               >  2:151445/1‑229 (MQ=255)                                                                                                                                                                                                                        ggctcTGGTGAAGCAGGCGGTTGATGATGAAGAGCGGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTGGCTACTACTTTGAAGCAGTTGCGCAGCTGCCGCAGGAAGCGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCACTCGGTCTGCC‑GGTGAAACGTTTTATTGCTGCGACCa                             <  2:415541/247‑1 (MQ=255)                                                                                                                                                                                                                               gggaagaaGGCTTTTGATGATGAAGAGCTGAAAGTGGCGCGGGGGTTAAAATCAGCTAACTCTATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCAGTTGCGCAGCTGCCGCAGGAAGCGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCACTCGGTCTGCC‑GGTGAAACGTTTTATTGCTGCGACCAACGTGaa                      <  2:121494/249‑1 (MQ=255)                                                                                                                                                                                                                                 gAAGCAGGCGTTTGATGATGAAGAGCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCAGTTGCGCAGCTGCCGCAGGAAGCGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCACTCGGTCTGCC‑GGTGAAACGTTTTATTGCTGCGACCAACGTGAAc                     <  1:205177/250‑1 (MQ=255)                                                                                                                                                                                                                                           gatgattaataagagCTGAAAGTGGGGCTGGGTTTAAACTCAGCTAACTACATTAATACAAGCCTTTTGATGGAGCAGTTTTGCATCTACTTTGAAGCAGTTGCGCAGCTGCCGCAGGAAGCGCGCACCCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCACTCGGTCTGCC‑GGTGAAACTTATTAGTGCTGCGTCCAACGTGAACGATACCGTGcc          <  2:251499/241‑1 (MQ=255)                                                                                                                                                                                                                                            ttGATGATGAAGAGCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCAGTTGCGCAGCTGCCGCAGGAAGCGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCACTCGGTCTGCC‑GGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGcc          <  1:393458/250‑1 (MQ=255)                                                                                                                                                                                                                                                   tgaAGAGCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCAGTTGCGCAGCTGCCGCAGGAAGCGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGGCTGCTGGGGGAGAGCCACGGTCCGCCCGGGGAAA‑GTTTTATTGCTGCGACCAAccggcacaaacacgcgccacgggtct  >  1:200525/1‑226 (MQ=255)                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                      ACCCATATTGCGGGTGATAAGCCAGTGACCATTCTGACCGCGACCTCCGGTGATACCGGAGCGGCAGTGGCTCATGCTTTCTACGGTTTACCGAATGTGAAAGTGGTTATCCTCTATCCACGAGGCAAAATCAGTCCACTGCAAGAAAAACTGTTCTGTACATTGGGCGGCAATATCGAAACTGTTGCCATCGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGAACTGAAAGTGGCGCTAGGGTTAAACTCGGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCACTCGGTCTGCC‑GGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCC  >  NC_000913/4088‑4580

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑