Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,543,047	T→G	intergenic (+414/‑68)	fimE → / → fimA	regulator for fimA/type 1 fimbriae major subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,543,047	0	T	G	100.0%	86.0 / NA	28	intergenic (+414/‑68)	fimE/fimA	regulator for fimA/type 1 fimbriae major subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (18/10);  total (18/10)

ATATGTAAAGCTAACGTTTCTGTGGCTCGACGCATCTTCCTCATTCTTCTCTCCAAAAACCACCTCATGCAATATAAACATCTATAAATAAAGATAACAATAGAATATTAAGCCAACAAATAAACTGAAAAAGTTTGTCCGCGATGCTTTCCTCTATGAGTCAAAATGGCCCCAAATGTTTCATCTTTTGGGGGAAAACTGTGCAGTGTTGGCAGTCAAACTCGTTTACAAAACAAAGTGTACAGAACGACTGCCCATGTCGATTTAGAAATAGTTTTTTGAAAGGAAAGCAGCATGAAAATTAAAACTCTGGCAATCGTTGTTCTGTCGGCTCTGTCCCTCAGTTCTACAGCGGCTCTGGCCGCTGCCACGACGGTTAATGGTGGGACCGTTCACTTTAAAGGGGAAGTTGTTAACGCCGCTTGCGCAGTTGATGCAGGCTCTGTTG  >  NC_000913/4542821‑4543268                                                                                                                                                                                                                                   |                                                                                                                                                                                                                              atatGCAAAGCTAACTTTTCTTTGGTTCGACGCATCTTCCTCATTCTTCACTCCAAAAACCACCTCATGCAATTTAAACATCTATAAATAAAGATAACAATAGAATATTAAGCCAACAAATAAACTGAAAAAGTTTTGCCGCGATGCTTTCCTCTATGAGTCAAAATGGCCCCAAATGTTTCATCTTCTGGGGGAAAACTTTGCAGTGTTTGCAGTCAAACTCGTTGACAAAACAAAGTGTACAGAACg                                                                                                                                                                                                         <  2:267520/249‑1 (MQ=255)               cGTTTCTGTGGCTCGCCGCATCTTCCTCATTCTTCTCTCCAAAAACCACCTCATGCAATATAAACATCTATAAATAAAGATAACAATAGAATATTAAGCCAACAAATAAACTGAAAAAGTTTGTCCGCGATGCTTTCCTCTATGAGTCAAAATGGCCCCAAATGTTTCATCTTTTGGGGGAAAACTGTGCAGTGTTGGCAGTCAAACTCGTTGACAAAACAAAGTGTACAGAACGACTGCCCATGTCGAtt                                                                                                                                                                                         >  2:50901/1‑251 (MQ=255)                 tttCTGTAGCTCGAAGCATCTTCCTCATTCTTCTGTCAAAAAACCACCACATGCAATATAAACTTCTATAAATAAAGATACCAATAGCCTATTAAGCCAACAAATAAACTGAAAACGTTTGTCCGCAAAGCTTTCATCTATGAGTCAAAATGGACCCAAATGTTTCATCTTTTGGGGGAAAACTGTACCGTGTTGGCAGTCAAACACGTTGACAAAAAAAAATGTAAAGAAAGACTGCCCATgacgtttt                                                                                                                                                                                        >  2:485237/1‑243 (MQ=255)                             gACGCATCTTCCTCATTCTTCTCGCCAAAAACCACCTCATGCAATATAAACATCTATAAATAAAGATAACAATAGAATATTAAGCCAACAAATAAACTGAAAAAGTTTGTCCGCGATGCTTTCCTCTATGAGTCAAAATGGCCCCAAATGTTTCATCTTTTGGGGGAAAACTGTGCAGTGGTGTCAGTCAAACTCTTTGACAAAACAAAGTGTACAGAACGGCTGCCCATGTCGATTTAGAAATAGtttt                                                                                                                                                                            <  2:453809/250‑1 (MQ=255)                                           aTTCTTCTCTCCAAAAACAACCTCATGCAATATAAACATCTATAAATAAAGATAACAATAGAATATTAAGCCAACAAATAAACTGAAAAAGTTTGTCCGCGATGCTTTCCTCTATGAGTCACAATGGCCCCAAATGTTTCATCTTTTGGGGGAAAACTGTGCAGTGTTGGCAGTCAAACTCGTTGACAAAACAAAGTGTACAGAACGACTGCCCATGTCGATTTAGAAATAGTTTTTTGAAAGGAAagc                                                                                                                                                               >  1:36717/1‑249 (MQ=255)                                                tctctcCAAAAACCACCTCATGCAATATAAACATCTATAAATAAAGATAACAATAGAATATTAAGCCAACAAATAAACTGAAAAAGTTTGTCCGCGATGCTTTCCTCTATGAGTCAAAATGGCCCCAAATGTTTCATCTTTTGGGGGAAAACTGTGCAGTGTTGGCAGTCAAACTCGTTGACAAAACAAAGTGTACAGAACGACTGCCCATGTCGATTTAGAAATAGTTTTTTGAAAGGAAAGCAGCATg                                                                                                                                                         <  1:69927/250‑1 (MQ=255)                                                     ccAAAAACAACCTCATGCAATATAAACATCTATAAATAAAGATAACAATAGAATATTAAGCCAACAAATAAACTGAAAAAGTTTGTCCGCGATGCTTTCCTCTATGAGTCAAAATGGCCCCAAATGTTTCCTCTTTTGGGGGAAAACTGTGCAGTGTTGGCAGTCAAACTCGTTGACAAAACCAAGTGTACAGAACGACTGCCCCTGTCGATTTAGAAATAGTTTTTTGAAAGGACAGCAGCAAGAAAAtt                                                                                                                                                   >  2:415746/1‑251 (MQ=255)                                                            acaccTCATGCAATATAAACATCTATAGATAAAGATAACAATAGAATATTAAGCCAACAAATAAACTGAAAAAGATGGTCAGCGATGCTTTCATCTATGAGTCAAAATGGCCCCAAATGTTTCATCTTTTGGGGGAAAACTGTGCAGAGTTGGCAGTCAAACTCGTTGCCAAAACAAAGTGTACAGCCCGACTGCCCATGTCGATTTAGAAATAGTTTTTTGAAAGGAAGGCAGCTTGAAACTTAAAActc                                                                                                                                            >  2:62069/2‑251 (MQ=255)                                                             caccTCATGCAATATAAACATCTATAAATAAAGATAACAATAGAATATTAAGCCAACAAATAAACTGAAAAAGTTTGTCCGCGATGCTTTCCTCTATGAGTCAAAATGGCCCCAAATGTTTCATCTTTTGGGGGAAAACTGTGCAGTGTTGGCAGTCAAACTCGTTGACAAAACAAAGTGTACAGAACGACTGCCCATGTCGATTTAGAAATAGTTTTTTGAAAGGAAAGCAGCATGAAAATTAAAActct                                                                                                                                           >  1:100537/1‑251 (MQ=255)                                                              accTCATGCAATATAAACATCTATAAATAAAGATAACAATAGAATAGTAAGCCAACAAAGAAACGGAAAAAGTTTGTCCGCGATGCTTTCCGCTATGAGTCAAAATGGCCCCAAATGTTTCAGCTTTTGGGGGAAAACTGTGCAGTGTTGGCAGTCAAACACATTGACAAAACAAAGTGTACAGAACGACTGCCCATGTCGATTTAGAAATAGTTTTTTGAAAGGAAAGCAGCATGAAAATTAAAACTCTg                                                                                                                                          <  1:288189/251‑1 (MQ=255)                                                                        atatAAACATCTATAAATAAAGATAACAATAGAATATTAAGCCAACAAATAAACTGAAAAAGTTTGTCCGTGATGCTTTCCTCTATGAGTCAAAATGGCCCCAAATGTTTCATCTTTTGGGGGAAAACTGTGCAGTGTTGGCAGTCAAACTCGTTGACAAAACAAAGTGTACAGAACGACTGCCCATGTCGATTTAGAAATAGTTTTTTGAAAGGAAAGCAGCATGAAAATTAAAACTCTGGCAATCgttg                                                                                                                                <  1:457117/251‑1 (MQ=255)                                                                        atatAAACATCTATAAATAAAGATAACAATAGAATATTAAGCCAACAAATAAACTGAAAAAGTTTGTCCGCGATGCTTTCCTCTATGAGTCAAAATGGCCCCAAATGTTTCATCTTTTGGGGGAAAACTGTGCAGTGTTGGCAGTCAAACTCGTTGACAAAACAAAGTGTACAGAACGACTGCCCATGTCGATTTAGAAATAGTTTTTTGAAAGGAAAGCAGCATGAAAATTAAAACTCTGGAAATCgttg                                                                                                                                >  1:425687/1‑251 (MQ=255)                                                                                                              aaGCCAACAAATAAACTGAAAAAGTTTGTCCGCGATGCTTTCCTCTATGAGTCAAAATGGCCCCAAATGTTTCATCTTTTGGGGGAAAACTGTGCAGTGTTGGCAGTCAAACTCGTTGACAAAACAAAGTGTACAGAACGACTGCCCATGTCGATTTAGAAATAGTTTTTTGAAAGGTAAGCAGCATGAAAATTAAAACTCTGGCAATCGTTGTTCTGTCGGCTCTGTCCCTCAGTTCTACAGCGGCTCTg                                                                                          >  1:141150/1‑251 (MQ=255)                                                                                                              aaGCCAACAAATAAACTGAAAAAGTGTGTCCGCGATGCTTTCCTCTATGAGTCAAAATGGCCCCAAATGTTTCATCTTTTGGGGGAAAACTGTGCAGTGTTGGCAGTCAAACTCGTTGACAAAACAAAGTGTACAGAACGACTGCCCATGTCGATTTAGAAATAGTTTTTTGAAAGGAAAGCAGCATGAAAATTAAAACTCTGGCAATCGTTGTTCTGTCGGCTCTGTCCCTCAGTTCTACAGCGGCTCTg                                                                                          <  2:308252/251‑1 (MQ=255)                                                                                                                     caaATAAACTGACAAAGTTTGTCCTCGATGCTTTCCTCTATGAGTCAAAATGGCCCCAAATGTTTCATCTTTTGTGGGAAAACTGTGCAGTGTTGGCAGTCACACTCGTTGCCAAAACAAAGTGTACAGAACGACTGCCCATGTCGATTTAGAAATAGTTTTTTGAAAGGAAAGCATCATGCAACTTCAAACTCTGGCAATCGTTGTTCTGTCGGCTCTGTCCCTCAGTTCTACAGCGGCTCTGGCCGCtc                                                                                   >  2:88379/1‑250 (MQ=255)                                                                                                                     caaATAAACTGAAAAAGTTTGTCCGCGATGCTTTCCTCTATGAGTCAAAATGACCACAAATGTTTCATCTTTTAGGGGAAAACTGTGCAGTGTTGGCAGTCAAACTCGTTGACAAAACAACGTGTACAGAAAAACTGACCATGTCGATTTAGAAATCATTTTTTGAAAGGAAAGCAGCATCAAAATTAAAACTCTGGAAATCGATGTTCTGTCGGCTCTGTCCTTCAGTTCTACAGCGGCTCTAGCCGCTg                                                                                   >  1:280288/1‑251 (MQ=255)                                                                                                                              tGAAAAAGTTTGTCCGCGATGCTTTCCTCTATGAGTCAAAATGGCCCCAAATGTTTCATCTTTTGGGGGAAAACTGTGCAGTGTTGGCAGTCAAACTCGTTGACAAAACAAAGTGTACAGAACGACTGCCCATGTCGATTTAGAAATAGTTTTTTGAAAGGAAAGCAGCATGAAAATTAAAACTCTGGCAATCGTTGTTCTGTCGGCTCTGTCCCTCAGTTCTACAGCGGCTCTGGCCGCTGCCACGACgg                                                                          >  1:25559/1‑251 (MQ=255)                                                                                                                               gAAAAAGTTTGTCCGCGATGCTTTCCTCTATGAGTCAAAATGGCCCCAAATGTTTCATCTTTTGGGGGAAAACTGTGCAGTGTTGGCAGTCAAACTCGTTGACAAAACAAAGTGTACAGAACGACTGCCCATGTCGATTTAGAAATAGTTTTTTGAAAGGAAAGCAGCATGAAAATTAAAACTCTGGCAATCGTTGTTCTGTCGGCTCTGTCCCTCAGTTCTACAGCGGCTCTGGCCGCTGCCacgacg                                                                           <  2:425687/249‑1 (MQ=255)                                                                                                                                  aaaGTTTGTCCGCGATGCTTTCCTCTATGAGTCAAAATGGCCCCAAATGTTTCATCTTTTGGGGGAAAACTGTGCAGTGTTGGCAGTCAAACTCGTTGACAAAACAAAGTGTACAGAACGACTGCCCATGTCGATTTAGAAATAGTTTTTTGAAAGGAAAGCAGCATGAAAATTAAAACTCTGGCAATCGTTGTTCTGTCGGCTCTGTCCCTCAGTTCTACAGCGGCTCTGGCCGCTGCCACGACGGTTa                                                                       >  2:477523/1‑250 (MQ=255)                                                                                                                                                         tctATGAGTCAAAATGGCCCCAAATGTTTCATCTTTTGGGGGAAAACTGTGCAGTGTTGGCAGTCAAACTCGTTGACAAAACAAAGTGTACAGAACGACTGCCCATGTCGATTTAGAAATAGTTTTTTGAAAGGAAAGCAGCATGAAAATTAAAACTCTGGCAATCGTTGTTCTGTCGGCTCTGTCCCTCAGTTCTACAGCGGCTCTGGCCGCTGCCACGACGGTTAATGGTGGGACCGTTCACTTTaa                                                 >  1:306593/1‑249 (MQ=255)                                                                                                                                                                     aaTGGCCCCAAATGTTTCATCTTTTGGGGGAAAACTGTGCAGTGTTGGCAGTCAAACTCGTTGACAAAACAAAGTGTACAGAACGACTGCCCATGTCGATTTAGAAATAGTTTTTTGAAAGGAAAGCAGCATGAAAATTAAAACTCTGGCAATCGTTGTTCTGTCGGCTCTGTCCCTCAGTTCTACAGCGGCTCTGGCCGCTGCCACGACGGTTAATGGTGGGACCGTTCACTTTAAAGGGGAAgttgtt                                    >  1:457623/1‑250 (MQ=255)                                                                                                                                                                       tGGCCCCAAATGTTTCATCTTTTGGGGGTAAACTGTGCAGTGTTGGCAGCCAAACTCGTTGACAAAACAAAGTGTACAGAACGACTGCCCATGTAGATTTAGAAATAGTTTTTTGAAAGGAAAGCAGCATGACAATTAAAACTCTGGCAATCGTTGTTCTGTCGGCTCTGTCCCTCACTTCTACAGCGGCTCTGGCCGCCGCCACGTCGGTTACTAGCGGGCCCGTTTCCTTTACAGGGGAAGTTGTTAAc                                 >  2:475359/1‑251 (MQ=255)                                                                                                                                                                         gCCCCAAATGTTTCATCTTTTGGGGGAAAACTGTGCAGTGTTGGCAGTCAAACTCGTTGACAAAACAAAGTGTACAGAACGACTGCCCATGTCGATTTAGAAATAGTTTTTTGAAAGGAAAGCAGCATGAAAATTAAAACTCTGGCAATCGTTGTTCTGTCGGCTCTGTCCCTCAGTTCTACAGCGGCTCTGGCCGCTGCCACGACGGTTAATGGTGGGACCGTTCACTTTAAAGGGGAAGTTGTTAAcgc                               <  1:346417/251‑1 (MQ=255)                                                                                                                                                                                   tttCATCTTTTGGGGGAAAACTGTGCAGTGTTGGCAGTCAAACTCGTTGACAAAACAAAGTGTACAGAACGACTGCCCATGTCGATTTAGAAATAGTTTTTTGAAAGGAAAGCAGCATGAAAATTAAAACTCTGGCAATCGTTGTTCTGTCGGCTCTGTCCCTCAGTTCTACAGCGGCTCTGGCCGCTGCCACGACGGTTAATGGTGGGACCGTTCACTTTAAAGGGGAAGTTGTTAACGCCGCTTgcgc                      >  1:136985/1‑250 (MQ=255)                                                                                                                                                                                    ttCATCTTTTGGGGGAAAACTGTGCAGTGTTGGCAGTCAAACTCGTTGACAAAACAAAGTGTACAGAACGACTGCCCATGTCGATTTAGAAATAGTTTTTTGAAAGGAAAGCAGCATGAAAATTAAAACTCTGGCAATCGTTGTTCTGTCGGCTCTGTCCCTCAGTTCTACAGCGGCTCTGGCCGCTGCCACGACGGTTAATGGTGGGACCGTTCACTTTAAAGGGGAAGTTGTTAACGCCGCTTGCGCa                     <  2:306593/250‑1 (MQ=255)                                                                                                                                                                                                gggAAAACTGTGCAGTGTTGGCAGTCAAACTCGTTGACAAAACAAAGTGTACAGAACGACTGCCCATGTCGATTTAGAAATAGTTTTTTGAAAGGAAAGCAGCATGAAAATTAAAACTCTGGCAATCGTTGTTCTGTCGGCTCTGTCCCTCAGTTCTACAGCGGCTCTGGCCGCTGCCACGACGGTTAATGGTGGGACCGTTCACTTTAAAGGGGAAGTTGTTAACGCCGCTTGCGCAGTTGATGCAGGct        >  1:245865/1‑251 (MQ=255)                                                                                                                                                                                                  gAAAACTGTGCAGTGTTGGCAGTCAAACTCGTTGACAAAACAAAGTGTACAGAACGACTGCCCATGTCGATTTAGAAATAGTTTTTTGAAAGGAAAGCAGCATGAAAATTAAAACTCTGGCAATCGTTGTTCTGTCGGCTCTGTCCCTCAGTTCTACAGCGGCTCTGGCCGCTGCAACGACGGTTAATGGTGGGACCGTTCACTTTAAAGGGGAAGTTGTTAACGCCGCTTGCGCAGTTGATGCAGGcgct      >  2:107266/1‑248 (MQ=255)                                                                                                                                                                                                      aCTGTGCAGTGTTGGCAGTCAAACTCGTTGACAAAACAAAGTGTACAGAACGACTGCCCATGTCGATTTAGAAATAGTTTTTTGAAAGGAAAGCAGCATGAAAATTAAAACTCTGGCAATCGTTGTTCTGTCGGCTCTGTCCCTCAGTTCTACAGCGGCTCTGGCCGCTGCCACGACGGTTAATGGTGGGACCGTTCACTTTAAAGGGGAAGTTGTTAACGCCGCTTGCGCAGTTGATGCAGGCTCTGTTg  <  1:297720/251‑1 (MQ=255)                                                                                                                                                                                                                                   |                                                                                                                                                                                                                              ATATGTAAAGCTAACGTTTCTGTGGCTCGACGCATCTTCCTCATTCTTCTCTCCAAAAACCACCTCATGCAATATAAACATCTATAAATAAAGATAACAATAGAATATTAAGCCAACAAATAAACTGAAAAAGTTTGTCCGCGATGCTTTCCTCTATGAGTCAAAATGGCCCCAAATGTTTCATCTTTTGGGGGAAAACTGTGCAGTGTTGGCAGTCAAACTCGTTTACAAAACAAAGTGTACAGAACGACTGCCCATGTCGATTTAGAAATAGTTTTTTGAAAGGAAAGCAGCATGAAAATTAAAACTCTGGCAATCGTTGTTCTGTCGGCTCTGTCCCTCAGTTCTACAGCGGCTCTGGCCGCTGCCACGACGGTTAATGGTGGGACCGTTCACTTTAAAGGGGAAGTTGTTAACGCCGCTTGCGCAGTTGATGCAGGCTCTGTTG  >  NC_000913/4542821‑4543268

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑