Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,517,127	C→A	V197V (GTG→GTT)	fecR ←	ferric citrate regulator FecR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,517,127	0	C	A	100.0%	173.8 / NA	54	V197V (GTG→GTT)	fecR	ferric citrate regulator FecR
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (21/33);  total (21/33)

CAGCCCGGCAACGGCGGGATCGCAGCGCAGCACGCCGTTGCGGTAACGGGTTAGCGTGGCTATCACCTCACCCAGCGGTTTATCGCTGAAGCTCAGGATGTCCTTCGTCCAGCTTGTACTCTCGTCATCCAGCGGTTTCACTGCGCCAAACTCAGAGGCGCTGAACTGCAGGCTTTCACCAGCGTTCACGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGTGCTGCTGCACGTCAAGCTGCGTGAAATTATCCTGCTGGCGGACGGTAAATTCTGTCCCTAAAGCAGTGAGCTGGCCCTGACGGGTCAGGACACGGAAGGGGCGTTGCAGGGCATCTTTCGCGGTAGTAATGGCGATTTCACCGTACCAGAGCCGGACGGTGCGCTGATGCGCATCAAAACGCACATCCGCCGCGCTTTGGGTATTCAGCGTGAGCAGGGAGCCATCTTCCAGTTGCTGACGGCTGAC  >  NC_000913/4516884‑4517366                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                cAGCCCTGCAACGGCGGGATCGCAGCGCAGCACGCCGTTGCGGTAACGGGTTAGCGTGGCTATCACCTCACCCAGTGGTTTATCGCTGAAGCTCAGGATGTCCTTCGTCCAGCTTGTACTCTCGTCATCCAGCGGTTTCACTGCGCCAAACTCAGAGGCGCTGAACTGCAGGCTTTCACCAGCGTTCACGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGTGCTGCTGAACGTCaa                                                                                                                                                                                                                                          <  2:414358/251‑1 (MQ=255) cAGCCCGGCAACGGCGGGATCGCAGCGCAGCTCGACGTTGCGGTAACGGGTTAGCGTGGCTATCACCTCACCCAGTGTGTTATCGCTGAAGCTCAGGATGTCCTTCGTCCAGCTTGTACTCTCGTCATCCAGCGGTTTCACTGCGCCAAACTCAGAGGCGCTGAACTGCAGGCTTTCACCAGCGTTCACGAGGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTTCACAGCGTGCTGCTGAACGTCaa                                                                                                                                                                                                                                          <  2:60384/251‑1 (MQ=255)  ggCCCGGCAACGGCGGGATCGCAGCGCAGCACGCCGTTGCGGTAACGGGTTAGCGTGGCTATCACCTCACCCAGTGGTTTATCGCTGAAGCTCAGGATGTCCTTCGTCCAGCTTGTACTCTCGTCATCCAGCGGTTTCACTGCGCCAAACTCAGAGGCGCTGAACTGCAGGCTTTCACCAGCGTTCACGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGTGCTGCTGAACGTCAAg                                                                                                                                                                                                                                         <  2:402709/250‑1 (MQ=255)     ccGGCAACGGCGGGATCGCAGCGCAGCACGCCGTTGCGGTTACGGGTTAGCGTGGCTATCCCCTCACCCAGTGGTTTATCGCTGAAGCTCAGGATGTCCTTCGTCCAGCTTGTACTCTCGGCATCCAGCGGTTTCACTGCGCCAAACTCAGAGGCGCTGAACTGCAGGCTTTCACCAGCGTTCACGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGTGCTGCTGAACGTCAAGCTg                                                                                                                                                                                                                                      <  2:52039/251‑1 (MQ=255)                 ggATCGCAGCGCAGCACGCCGTTGCGGTAACGGGTTAGCGTGGCTATCACCTCACCCAGTGGTTTATCGCTGAAGCTCAGGATGTCCTTCGTCCAGCTTGTACTCTCGTCATCCAGCGGTTTCACTGCGCCAAACTCAGAGGCGCTGAACTGCAGGCTTTCACCAGCGTTCACGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGTGCTGCTGAACGTCAAGCTGCGAGAAATTATc                                                                                                                                                                                                                          >  1:170879/1‑251 (MQ=255)                    tCGCCGCGCAGCACGCCGTTGCGGTAACGGGTTAGCGTGGCTATCACCTCCCCCAGTGGTTTATCGCTGAAGCTCAGGATGTCCTTCGTCCAGCTTGTACTCTCGTCATCCAGCGGTTTCACTGCGCCAAACTCAGAGGCGCTGAACTGCAGGCTTTCACCAGCGTTCACGATGCGTTTTGGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGTGCTGCTGAACGTCAAGCTGCGTGAAATTATCct                                                                                                                                                                                                                        <  2:248026/250‑1 (MQ=255)                       cagcgcagcACGCCGTGGCGGTAACGGGTTAGCGTGGCTATCACCTCACCCAGTGGTTTATCGCTGAAGCTCAGGATGTCCTTCGTCCAGCTTGTACTCTCGTCATCCAGCGGTTTCACTGCGCCAAACTCAGAGGCGCTGAACTGCAGGCTTTCACCAGCGTTCACGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGTGCTGCTGAACGTCAAGCTGCGTGAAATTATCctgctg                                                                                                                                                                                                                    <  2:214068/251‑1 (MQ=255)                         gcgccgcACGCCGTTGCGGGAACGGGTTAGCGTGGCGATCCCCTCACCCAGTGGTTTATCGCTGAAGCTCAGGATGTCCTGCGTCCAGCTTGTACTCGCGTCATCCAGCGGTTTCACTGCGCCAAACTCAGAGGCGCTGAACTGCAGGCTTTCACCAGCGTTCACGATGCTGTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGTGCTGCTGAACGTCAAGCTGCGTGAAATTATCCTGCTGGc                                                                                                                                                                                                                  <  2:446069/251‑1 (MQ=255)                           gcagcaCGCCGTTGCGGTAACGGGTTAGCGTGGCTATCACCTCACCCAGTGGTTTATCGCTGAAGCTCAGGATGTCCTTCGTCCAGCTTGTACTCTCGTCATCCAGCGGTTTCACTGCGCCAAACTCAGAGGCGCTGAACTGCAGGCTTTCACCAGCGTTCACGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTACAACAGCGTGCTGCTGAACGTCAAGCTACGTGAAATTATCCTgcgggcgg                                                                                                                                                                                                                >  1:50898/1‑251 (MQ=255)                                  gCCGTTGCGGTAACGGGTTAGCGTGGCGATCACCTCACCCAGTGGTTTACCGCTGAAGCTCAGGATGTCCTTCGTCCAGCTTGTACTCTCGTCATCCAGCGGTTTCACTGCGCCAAACTCAGAGGCGCTGAACTGCAGGCTTTCACCAGCGTTCACGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGTGCTGCTGAACGTCAAGCTGCGTGAAATTATCCTGCTGGCGGACGGTaa                                                                                                                                                                                                         <  2:248281/251‑1 (MQ=255)                                    cGTTGCGGTAACGGGTTAGCGTGGCTATCACCTCACCCAGTGGTTTATCGCTGAAGCTCAGGATGTCCTTCGTCCAGCTTGTACTCTCGTCATCCAGCGGTTTCACTGCGCCAACCTCAGAGGCGCTGAACTGCAGGCTTTCACCAGCGTTCACGATGCGTTTTTGCGCGGGGGCACTGGCGCGAAGCACTTCCACAGCGTGCTGCTGAACGTCAAGCTCCGCGAAATTATCCTGCTGGCGGCCGGTAAAt                                                                                                                                                                                                       >  1:206378/1‑251 (MQ=255)                                     gTTGCGGTAACGGGTTAGCGTGGCTATCACCTCACCCAGTGGTTTATCGCTGAAGCTCAGGATGTCCTTCGTCCAGCTTGTACTCTCGTCATCCAGCGGTTTCACTGCGCCAAACTCAGAGGCGCTGAACTGCAGGCTTTCACCAGCGTTCACGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGTGCTGCTGAACGTCAAGCTGCGTGAACTTATCCTGCTGGCGGACGGTAAAtt                                                                                                                                                                                                      >  2:456424/1‑251 (MQ=255)                                     gTTGCGGTAACGGGTTAGCGTGGCTATCACCTCACCCAGTGGTTTATCGCTGAAGCTCAGGATGTCCTTCGTCCAGCTTGTACTCTCGGCATCCAGCGGTTTCACTGCGCCAAACTCAGAGGCGCTGAACTGCAGGCTTTCACCAGCGTTCACGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGTGCTGCTGAACGTCAAGCTGCGTGAAATTATCCTGCTGGCGGACGGTAAAtt                                                                                                                                                                                                      <  2:149611/251‑1 (MQ=255)                                         cGGTAACGGGTTAGCGTGGCTATCACCTCACCCAGTGGTTTATCGCTGAAGCTCAGGATGTCCTTCGTCCAGCTTGTACTCTCGTCATCCAGCGGTTTCACTGCGCCAAACTCAGAGGCGCTGAACTGCAGGCTTTCACCAGCGTTCACGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGTGCTGCTGAACGTCAAGCTGCGTGAAATTATCCTGCTGGCGGACGGTAAATTCTGt                                                                                                                                                                                                  <  2:344185/251‑1 (MQ=255)                                          ggTAACGGGTTAGCGTGGCTATCACCTCACCCAGTGGTTTATCGCTGAAGCTCAGGATGTCCTTCGTCCAGCTTGTACTCTCGTCATCCAGCGGTTTCACTGCGCCAAACTCAGAGGCGCTGAACTGCAGGCTTTCACCAGCGTTCACGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGTGCTGCTGAACGTCAAGCTGCGTGAAATTATCCTGCTGGCGGACGGTAAATTCTGTc                                                                                                                                                                                                 <  1:406179/251‑1 (MQ=255)                                             accGGGTTAGCGTGGCTATCACCTCACCCAGTGGTTTATCGCTGAAGCTCAGGATGTCCTTCGTCCAGCTTGTACTCTCGTCATCCAGCGGTTTCACTGCGCCAAACTCAGAGGCGCTGAACTGCAGGCTTTCACCAGCGTTCACGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGTGCTGCTGAACGTCAAGCTGCGTGAAATTATCCTGCTGGCGGACGGTAAATTCTGTcccc                                                                                                                                                                                              >  2:103715/3‑250 (MQ=255)                                                      gcggtgCTATCACTGCACCCAGTGGTTTATCGCTGAAGCTCATGATGTCCTTCGTCCAGCTTGTACTCTCGTCATCCAGCGGGTTCACTGCGCCAAACTCAGAGGCGCTGAACTGCAGGCTTTTACCAGCGTTCACGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGGGCTGCTGAACGTCAAGCTGCGTGAAATTATCCTGCTGGCGGACGGTAAATCCTGTCCCTAAAGCAGTg                                                                                                                                                                                     <  2:468050/246‑1 (MQ=255)                                                       cGTGGCTATCCCCTCACCCAGTGGTTTATCGCTGAAGCTCAGGATGTCCTTCGTCCAGCTTGTACTATCGTCATCCAGCGGTTTCACTGCGCCAAACTCAGAGGCGCTGAACTGCAGGCTTTCACCAGCGTTCACGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGTGCTGCTGAACGTCAAGCTGCGTGAAATTATCCTGCTGGCGGACGGTAAATTCTTTCCCTAAAGCAGTGa                                                                                                                                                                                    <  1:264131/251‑1 (MQ=255)                                                              aTCACCTCACCCAGTTGTTTATCGCTGAAGCTCAGGATGTCCTTCGTCCAGCTTGTACTCTCGTCATCCAGCGGTTTCACTGCGCCAAACTCAGAGGCGCTGAACTGCAGGCTTTCACCAGCGTTCACGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGTGCTGCTGAACGTCAAGCTGCGTGAAATTATCCTGCTGGCGGACGGTAAATTCTGTCCCTAAAGCAGTGAGCTGGc                                                                                                                                                                              <  1:33835/250‑1 (MQ=255)                                                                         cggtggTTTATCGCTGAAGTGCAGGTTGTCCTTCGTCCAGCTGGTACTCCCGCCATCCAGCTGTTTCACTGCGCCAAACTCAGAGGCGCTGAACTGCAGGCTTTCACCAGCGTTCACGATGCGGTTTTGCGCGGGGGCACTGGCGATAAGCACTTCCACAGCGGGCTGCTTAACGTCAAGCTGCGTGAAATTATCCTGCTGGCGGACGTGAAATTCTTTCCCTAAAGCAGTGCGCTGGCCCTGTTGGGTCa                                                                                                                                                                  <  2:459196/247‑1 (MQ=255)                                                                                ttATCGCTGAAGCTCAGGATGTCCTTCGTCCAGCTTGTACTCTCGTCATCCAGCGGTTTCACTGCGCCAAACTCAGAGGCGCTGAACTGCAGGCTTTCACCAGCGTTCACGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGTGCTGCTGAACGTCAAGCTGCGTGAAATTATCCTGCTGGCGGACGGTAAATTCTGTCCCTAAAGCAGTGAGCTGGCCCTGACGGGTCAGGacac                                                                                                                                                            <  1:79599/250‑1 (MQ=255)                                                                                       tGAAGCTCAGGATGTCCTTCGTCCAGCTTGTACTCTCGTCATCCAGCGGTTTCACTGCGCCAAACTCAGAGGCGCTGAACTGCAGGCTTTCACCAGCGTTCACGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGTGCTGCTGAACGTCAAGCTGCGTGAAATTATCCTGCTGGCGGACGGTAAATTCTGTCCCTAAAGCAGTGAGCTGGCCCTGACGGGTCAGGACACGGAAgggg                                                                                                                                                    >  1:98465/1‑251 (MQ=255)                                                                                        gAAGCTCAGGATGTCCTTCGTCCAGCTTGTACTCTCGTCATCCAGCGGTTTCACTGCGCCAAACTCAGAGGCGCTGAACTGCAGGCTTTCACCAGCGTTCACGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGTGCTGCTGAACGTCAAGCTGCGTGAAATTATCCTGCTGGCGGACGGTAAATTCTGTCCCTAAAGCAGTGAGCTGGCCCTGACGGGTCAGGACACGGAAggg                                                                                                                                                     <  1:182130/249‑1 (MQ=255)                                                                                          aGCTCAGGATGTCCTTCGTCCAGCTTGTACTCTCGTCATCCAGCGGTTTCACTGCGCCAAACTCAGAGGCGCTGAACTGCAGGCTTTCACCAGCGTTCACGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGTGCTGCTGAACGTCAAGCTGCGTGAAATTATCCTGCTGGCGGACGGTAAATTCTGTCCCTAAAGCAGTGAGCTGGCCCTGACGGGTCAGGACACGGAAGGGGCGt                                                                                                                                                 <  2:398436/251‑1 (MQ=255)                                                                                                           gaccAGCTTGTACTCTCGTCATCAAGCGGTTTCACTGCGCCAAACTCAGAGGCGCTGAACTGCAGGCTTTCACCAGCGTTCACGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGTGCTGCTGAACGTCAAGCTGCGTGAAATTATCCTGATGGCGGACGGTAAATTCTGTCCCTAAAGCAGTGAGCTGGCCCTGACGGGTCAGGCCGCGGAAGGAGCGTTGCAGGGCATATTTcgc                                                                                                                                >  1:99394/3‑251 (MQ=255)                                                                                                             ccAGCTTGTACTCTCGTCATCCAGCGGTTTCACTGCGCCAAACTCAGAGGCGCTGAACTGCAGGCTTTCACCAGCGTTCACGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGTGCTGCTGAACGTCAAGCTGCGTGAAATTATCCTGCTGGCGGACGGTAAATTCTGTCCCTAAAGCAGTGAGATGGCCCTGACGGGTCAGGACACGGAAGGGGCGTTGCAGGGCATCTTTCGCgg                                                                                                                              >  2:402638/1‑251 (MQ=255)                                                                                                              cAGCTTGTCCTCTCGCCATCTAGCGGTTTCACTGCGCCAAACTCAGAGGCGCTGAACTGCAGGCCTTCACCAGCGTTCACCATGCGTTTTTGCGCGGGGGCACCGGCGAGAAGCACTTCCACAGCCTGCTGCCGAACGTCAAGCTGCGTGAAAGTATCCTGCTGGCGGACTGTAAATTCTGTCCCTAAAGTAGTGAGCTGGCCCTGACGGGTCAGGCCACGGAAGGGGCGTTGCAGGGCATCTTTCGCGgt                                                                                                                             <  1:230774/251‑1 (MQ=255)                                                                                                               aGCTTGTACTCTCGTCATCCAGCGGTTTCACTGCGCCAAACTCAGAGGCGCTGAACTGCAGGCTTTCACCAGCGTTCACGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGTGCTGCTGAACGTCAAGCTGCGTGAAATTATCCTGCTGGCGGACGGTAAATTCTGTCCCTAAAGCAGTGAGCTGGCCCTGACGGGTCAGGACACGGAAGGGGCGTTGCAGGGCATCTTTCGCGgt                                                                                                                             >  1:77485/1‑250 (MQ=255)                                                                                                                 cTTGTACTCTCGTCATCCAGCGGTTTCACTGCGCCAAACTCAGAGGCGCTGAACTGCAGGCTTTCACCAGCGTTCACGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGTGCTGCTGAACGTCAAGCTGCGTGAAATTATCCTGCTGGCGGACGGTAAATTCTGTCCCTAAAGCAGTGAGCTGGCCCTGACGGGTCAGGACACGGAAGGGGCGTTGCAGGGCATCTTTCGCGgtagt                                                                                                                          >  1:271456/1‑251 (MQ=255)                                                                                                                    gTACTCTCGTCATCCAGCGGTTTCACTGCGCCAAACTCAGAGGCGCTGAACTGCAGGCTTTCACCAGCGTTCACGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGTGCTGCTGAACGTCAAGCTGCGTGAAATTATCCTGCTGGCGGACGGTAAATTCTGTCCCTAAAGCAGTGAGCTGGCCCTGACGGGTCAGGACACGGAAGGGTCGTTGCAGGGCATCTTTCGCGGTAGTaa                                                                                                                        <  1:417092/250‑1 (MQ=255)                                                                                                                            gTCATCCAGCGGTTTCACTGCGCCAAACTCAGAGGCGCTGAACTGCAGGCTTTCACCAGCGTTCACGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGTGCTGCTGAACGTCAAGCTGCGTGAAATTATCCTGCTGGCGGACGGTAAATTCTGTCCCTAAAGCAGTGAGCTGGCCCTGACGGGTCAGGACACGGAAGGGGCGTTGCAGGGCATCTTTCGCGGTAGTAATGGCGAttt                                                                                                               <  2:75401/251‑1 (MQ=255)                                                                                                                              aaTCCATCGGTTTCACTGCGCCAAACTCATAGGCGCTGAACTGCAGGCTTTCACCTGCGTTCACGATTCGTTTTTGCGCGGGGGCGCTGGCGAGAAGCACTTCCACAGCGTGCTGCTGAACGTCAAGCTGCGTGAAATTATCCTGCTGGCGGACGGTAAATGCTGTCCCTAAAGCAGTGAGCTGGCCCTGACGGGTCAGGACACGGAAGGGGCGTTGCAGGGCATCTTTCGCGGTAGTAATGGCGATTTc                                                                                                              <  2:355541/249‑1 (MQ=255)                                                                                                                                           cACTGCGCCAAACTCAGAGGCGCTGAACTGCAGGCTTTCACCAGCGTTCACGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGTGCTGCTGAACGTCAAGCTGCGTGAAATTATCCTGCTGGCGGACGGTAAATTCTGTCCCTAAAGCAGTGAGCTGGCCCTGACGGGTCAGGACACGGAAGGGGCGTTGCAGGGCATCTTTCGCGGTAGTAATGGCGATTTCACCGTACCAGAGc                                                                                                 <  1:2821/250‑1 (MQ=255)                                                                                                                                                  ccAAACTCAGAGGCGCTGAACTGCAGGCTTTCACCAGCGTTCACGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGTGCTGCTGAACGTCAAGCTGCGTGAAATTATCCTGCTGGCGGACGGTAAATTCTGTCCCTAAAGCAGTGAGCTGGCCCTGACGGGTCAGGACACGGAAGGGGCGTTGCAGGGCATCTTTCGCGGTAGTAATGGCGATTTCACCGTACCAGAGCCGGACGGt                                                                                         <  1:103715/251‑1 (MQ=255)                                                                                                                                                    aaaCTCAGAGGCGCTGAACTGCAGGCTTTCACCAGCGTTCACGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGTGCTGCTGAACGTCAAGCTGCGTGAAATTATCCTGCTGGCGGACGGTAAATTCTGTCCCTAAAGCAGTGAGCTGGCCCTGACGGGTCAGGACACGGAAGGGGCGTTGCAGGGCATCTTTCGCGGTAGTAATGGCGATTTCACCGTACCAGAGCCGGACGGTgc                                                                                       >  1:376373/1‑251 (MQ=255)                                                                                                                                                     aaCTCAGCGGCGCTGAACTGCAGGCTTTCACCAGCGTTCACGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGTGCTGCGTAACGTCAAGCTGCGGGAAATTATCCTGCTGGCGGACGGTAAATTCTGTCCCTAAAGCAGTGAGCTGGCCCTGACGGGTCAGGACACGGAAGGGGCGTTGCAGGGCATCGTTCGCGGTAGTAATGGCGATTTCACCGTACCAGAGCCGGACGGTgcg                                                                                      <  2:469616/251‑1 (MQ=255)                                                                                                                                                               cgcTGAACTGCAGGCTTTCACCAGCGTTCACGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGTGCTGCTGAACGTCAAGCTGCGTGAAATTATCCTGCTGGCGGACGGTAAATTCTGTCCCTAAAGCAGTGAGCTGGCCCTGACGGGTCAGGACACGGAAGGGGCGTTGCAGGGCATCTTTCGCGGTAGTAATGGCGATTTCACCGTACCAGAGCCGGACGGTGCGCTGATgcgc                                                                             >  1:249898/1‑250 (MQ=255)                                                                                                                                                                gcTGAACTGCAGGCTTTCACCAGCGTTCACGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGTGCTGCTGAACGTCAAGCTGCGTGAAATTATCCTGCTGGCGGACGGTAAATTCTGTCCCTAAAGCAGTGAGCTGGCCCTGACGGGTCAGGACACGGAAGGGGCGTTGCAGGGCATCTTTCGCGGTAGTAATGGCGATTTCACCGTACCAGAGCCGGACGGTGCGCTGATGCGCAt                                                                           <  1:303300/251‑1 (MQ=255)                                                                                                                                                                  tGAACTGCAGGCTTTCACCAGCGTTCACGATGAGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGTGCTGCTGAACGTCAAGCTGCGTGAAATTATCCTGCTGGCGGACGGTAAATTCTGTCCCTAAAGCAGTGAGCTGGCCCTGACGGGTCAGGACACGGAAGGGGCGTTGCAGGGCATCTTTCGCGGTAGTAATGGCGATTTCACCGTACCAGAGCCGGACGGTGCGCTGGTGCGCATc                                                                          >  1:53682/1‑250 (MQ=255)                                                                                                                                                                        gCAGGCTTTCACCAGCGTTCACGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGTGCTGCTGAACGTCAAGCTGCGTGAAATTATCCTGCTGGCGGACGGTAAATTCTGTCCCTAAAGCAGTGAGCTGGCCCTGACGGGTCAGGACACGGAAGGGGCGTTGCAGGGCATCTTTCGCGGTAGTAATGGCGATTTCACCGTACCAGAGCCGGACGGTGCGCTGATGCGCATCAAAACGc                                                                   >  1:224186/1‑251 (MQ=255)                                                                                                                                                                                        gTTCACGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGTGCTGCTGAACGTCAAGCTGCGTGAAATTATCCTGCTGGCGGACGGTAAATTCTGTCCCTAAAGCAGTGAGCTGGCCCTGACGGGTCAGGACACGGAAGGGGCGTTGCAGGGCATCTTTCGCGGTAGTAATGGCGATTTCACCGTACCAGAGCCGGACGGTGCGCTGATGCGCATCAAAACGCACATCCGCCGCGCttt                                                   <  1:242815/251‑1 (MQ=255)                                                                                                                                                                                           cACGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGTGCTGCTGAACGTCAAGCTGCGTGAAATTATCCTGCTGGCGGACGGTAAATTCTGTCCCTAAAGCAGTGAGCTGGCCCTGACGGGTCAGGACACGGAAGGGGCGTTGCAGGGCATCTTTCGCGGTAGTAATGGCGATTTCACCGTACCAGAGCCGGACGGTGCGCTGATGCGCATCAAAACGCACATCCGCCGCGCTTTggg                                                <  1:374175/251‑1 (MQ=255)                                                                                                                                                                                            aCGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGTGCTGCTGAACGTCAAGCTGCGTGAAATTATCCTGCTGGCGGACGGTAAATTCTGTCCCTAAAGCAGTGAGCTGGCCCTGACGGGTCAGGACACGGAAGGGGCGTTGCAGGGCATCTTTCGCGGTAGTAATGGCGATTTCACCGTACCAGAGCCGGACGGTGCGCTGATGCGCATCAAAACGCACATCCGCCGCGCTTTGGGt                                               <  1:402638/251‑1 (MQ=255)                                                                                                                                                                                                         gcgcGGGGGCACTGGCGAGAACCACTTCCACACAGGTCTGCGGACCGTCAAGCTGCGTGAAATTATCCTGCTGGCGGACGGTAAATACTGTCCTTAAAGCAGTGAGCTGGCCATGACGGTTCAGGACCCGGAAGGGGCGTTGCAGGGCATCTTCCGCGGTAGTAATGGCGATTTCACCGTCCCAGAGCCGGACGGTGCGCTGATGCGCATCAAAACGCACACCCGCCGCGCTTTGGGTATTCAGCGTGAg                                   <  2:379300/250‑1 (MQ=255)                                                                                                                                                                                                          cgcgGGGGCACTGGCGAGAAGCACTTCCACAGCGTGCTGCTGAACGTCAAGCTGCGTGAAATTATCCTGCTGGCGGACGGTAAATTCTGTCCCTAAAGCAGTGAGCTGGCCCTGACGGGTCAGGACACGGAAGGAGCGTTGCAGGGCATCTTTCGCGGTAGTAATGGCGATTTCACCGTACCAGAGCCGGACGGTGCGCTGATGCGCATCAAAACGCACATCCGCCGCGCTTTGGGTGTTCAGCGTGAGc                                  >  1:399430/1‑250 (MQ=255)                                                                                                                                                                                                               gggCACTGGCGAGAAGCACTTCCACCGCGTGCTGCTGAACGTCAAGCTGCGTGAAATTATCCTGCTGGCGGACGGTAAATTCTGTCCCTAAAGCAGTGAGCTGGCCCTGACGGGTCAGGACACTGAAGGGGCGTTGCAGGGCATCTTTCGCGGTAGTAATGGCGATTTCACCGTACCAGAGCCGGACGGTGCGCTGCTGCGCATCAACACGCCCATCCGCCGCGCTTTGGGTTTTCAGCGTGAGCaagggg                            >  2:332995/1‑246 (MQ=255)                                                                                                                                                                                                                         gagaAGCACTTCCACAGCGTGCTGCTGAACGTCAAGCTGCGTGAAATTATCCTGCTGGCGGACGGTAAATTCTGTCCCTAAAGCAGTGAGCTGGCCCTGACGGGTCAGGACACGGAAGGGGCGTTGCAGGGCATCTTTCGCGGTAGTAATGGCGATTTCACCGTACCAGAGCCGGACGGTGCGCTGATGCGCATCAAAACGCACATccgcc                                                          >  1:452753/1‑211 (MQ=255)                                                                                                                                                                                                                         gagaAGCACTTCCACAGCGTGCTGCTGAACGTCAAGCTGCGTGAAATTATCCTGCTGGCGGACGGTAAATTCTGTCCCTAAAGCAGTGAGCTGGCCCTGACGGGTCAGGACACGGAAGGGGCGTTGCAGGGCATCTTTCGCGGTAGTAATGGCGATTTCACCGTACCAGAGCCGGACGGTGCGCTGATGCGCATCAAAACGCACATccgcc                                                          <  2:452753/211‑1 (MQ=255)                                                                                                                                                                                                                             aGCACTTCCACAGCGTGCTGCTGAACGTCAAGCTGCGTGAAATTATCCTGCTGGCGGACGGTAAATTCTGTCCCTAAAGCAGTGAGCTGGCCCTGACGGGTCAGGACACGGAAGGGGCGTTGCAGGGCATCTTTCGCGGTAGTAATGGCGATTTCACCGTACCAGAGCCGGACGGTGCGCTGATGCGCATCAAAACGCACATCCGCCGCGCTTTGGGTATTCAGCGTGAGCAGGGAGCCATCTTCCAGTTg              <  1:240152/251‑1 (MQ=255)                                                                                                                                                                                                                               cACTTCCACAGCGTGCTGCTGAACGTCAAGCTGCGTGAAATTATCCTGCTGGCGGACGGTAACTTCTGTCCCTAAAGCAGTGAGCTGGCCCTGACGGGTCAGGACACGGAAGGGGCGTTGCAGGGCATCTTTCGCGGTAGTAATGGCGATTTCACCGTACCAGAGCCGGACGGTGCGCTGATGCGCATCAAAACGCACATCCGCCGCGCTTTGGGTATTCAGCGTGAGCAGGGAGCCATCTTCCAGTTGCt            >  1:123634/1‑251 (MQ=255)                                                                                                                                                                                                                                    ccACAGCGTGCTGCTGAACGTCAAGCTGCGTGAAATTATCCTGCTGGCGGACGGTAAATTCTGTCCCTAAAGCAGTGAGCTGGCCCTGACGGGTCAGGACACGGAAGGGGCGTTGCAGGGCATCTTTCGCGGTAGTAATGGCGATTTCACCGTACCAGAGCCGGACGGTGCGCTGATGCGCATCAAAACGCACATCCGCCGCGCTTTGGGTATTCAGCGTGAGCAGGGAGCCATCTTCCAGTTGCTGACgg       >  2:418291/1‑251 (MQ=255)                                                                                                                                                                                                                                      acaGCGTGCTGCTGAACGTCAAGCTGCGTGAAATTATCCTGCTGGCGGACGGTAAATTCTGTCCCTAAAGCAGTGAGCTGGCCCTGACGGGTCAGGACACGGAAGGGGCGTTGCAGGGCATCTTTCGCGGTAGTAATGGCGATTTCACCGTACCAGAGCCGGACGGTGCGCTGATGCGCATCAAAACGCACATCCGCCGCGCTTTGGGTATTCAGCGTGAGCAGGGAGCCATCTTCCAGTTGCTGACGGCt     >  2:471824/1‑251 (MQ=255)                                                                                                                                                                                                                                      acaGCGTGCTGCTGAACGTCAAGCTGCGTGAAATTATCCTGCTGGCGGACGGTAAATTCTGTCCCTAAAGCAGTGAGCTGGCCCTGACGGGTCAGGACACGGAAGGGGCGTTGCAGGGCATCTTTCGCGGTAGTAATGGCGATTTCACCGTACCAGAGCCGGACGGTGCGCTGATGCGCATCAAAACGCACATCCGCCGCGCTTTGGGTATTCAGCGTGAGCAGGGAGCCATCTTCCAGTTGCTGACGGCt     >  1:397674/1‑251 (MQ=255)                                                                                                                                                                                                                                         gCGTGCTGCTGAGCGTCAAGCTGCGTGAAATTATCCTGGTGGCGGACGGTAAATTCTGTCCCTAAAGCAGTGCGCCGGCCCTTACGGGTCAGGACCCGGAAGGGGCGTTGCAGGGCATCGTTCGCGGTAGTAATGGCGATTTCTCCGTACCAGAGCCGGACGGTGCGCGGATGCGTATCAAAACGCACATCCGCCGCGCTTTGGGTATTCAGCGTGAGCAGGGAGCCATCTTCCAGTTGCTGACGGCTGAc  <  2:53682/251‑1 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                CAGCCCGGCAACGGCGGGATCGCAGCGCAGCACGCCGTTGCGGTAACGGGTTAGCGTGGCTATCACCTCACCCAGCGGTTTATCGCTGAAGCTCAGGATGTCCTTCGTCCAGCTTGTACTCTCGTCATCCAGCGGTTTCACTGCGCCAAACTCAGAGGCGCTGAACTGCAGGCTTTCACCAGCGTTCACGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGTGCTGCTGCACGTCAAGCTGCGTGAAATTATCCTGCTGGCGGACGGTAAATTCTGTCCCTAAAGCAGTGAGCTGGCCCTGACGGGTCAGGACACGGAAGGGGCGTTGCAGGGCATCTTTCGCGGTAGTAATGGCGATTTCACCGTACCAGAGCCGGACGGTGCGCTGATGCGCATCAAAACGCACATCCGCCGCGCTTTGGGTATTCAGCGTGAGCAGGGAGCCATCTTCCAGTTGCTGACGGCTGAC  >  NC_000913/4516884‑4517366

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑