Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,937	A→G	P68P (CCA→CCG)	thrC →	threonine synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,937	0	A	G	97.4%	114.1 / NA	39	P68P (CCA→CCG)	thrC	threonine synthase
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base G (17/21);  total (18/21)
Fisher's exact test for biased strand distribution p-value = 4.62e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

GCTGGATACGGCGGGCGCACGAGTACTGGAAAACTAAATGAAACTCTACAATCTGAAAGATCACAACGAGCAGGTCAGCTTTGCGCAAGCCGTAACCCAGGGGTTGGGCAAAAATCAGGGGCTGTTTTTTCCGCACGACCTGCCGGAATTCAGCCTGACTGAAATTGATGAGATGCTGAAGCTGGATTTTGTCACCCGCAGTGCGAAGATCCTCTCGGCGTTTATTGGTGATGAAATCCCACAGGAAATCCTGGAAGAGCGCGTGCGCGCGGCGTTTGCCTTCCCGGCTCCGGTCGCCAATGTTGAAAGCGATGTCGGTTGTCTGGAATTGTTCCACGGGCCAACGCTGGCATTTAAAGATTTCGGCGGTCGCTTTATGGCACAAATGCTGACCCATATTGCGGGTGATAAGCCAGTGACCATTCTGACCGCGACCTCCGGTGATACCGGAGCGGCAGTGGCTCATGCTTTCTACGGTTTA  >  NC_000913/3697‑4177                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                 gcgggATACGGCGGGCGCACGAGTACTGGAAAACTAAATGAAACTCTACAATCTGAAAGATCACAAGGAGCAGGTCAGCTTTGCGCAAGCCGTAACCCAGGGGTTGGGCAAAAATCAGGGGCTGTTTTTTCCGCACGACCTGCCGGAATTCAGCCTGACTGAAATTGATGAGATGCTGAAGCTGGATTTTGTCACCCGCAGTGCGAAGATCCTCTCGGCGTTTATTGGTGATGAAATCCCGCAGGAAATcc                                                                                                                                                                                                                                        <  2:68115/248‑1 (MQ=255)    ggATACGGCGGGCGCACGAGTACTGGAAAACTAAATGAAACTCTACAATCTGAAAGATCACAATGAGCAGGTCAGCTTTGCGCAAGCCGTAACCCAGGGGTTGGGCAAAAATCAGGGGCTGTTTTTTCCGCACGACCTGCCGGAATTCAGCCTGACTGAAATTGATGAGATGCTGAAGCTGGATTTTGTCACCCGCAGTGCGAAGATCCTCTCGGCGTTTATTGGTGATGAAATCCCGCAGGAAATCCTgg                                                                                                                                                                                                                                     <  1:269836/251‑1 (MQ=255)         cggcggGCGCCCGCGTACTTGAAAACTAAATGAAACTCTACAAGCTGGAAGATCACCATGAGCAGGTCAGCTTTGCGCAAGCCGTAACCCCGGGGTTGGGCAAAAATCAGGGGCTGTTTTTTCCGCACGACCTGCCGGACTTAAGCCTGGCTTAAATTTATGAGGTTCTTAAGCTGGAGTTTGCTACCCGCAATGCGAAGAACCTTTCGGCGTTTATTTGTGATGAAATCCCGCAGGacacccagtaaa                                                                                                                                                                                                                                  >  2:74165/1‑238 (MQ=255)                    agAGTACTCGAAAACTACTTGAATCTCTACAATCTGAAAGATAACAATGAGCAGGGCAGCTTTGCGCAAGCCGTAACCCAGGGGTTGGGCAAAAATAAGGGGCTGTTTTCTCCGCCAGACATGCCGGACTTCAGCCTGACTGCAATTGATGCGATGCTGACGCTGGCTTTTGTCACCCGATGTGCGAAGTTCCTCTCGGCGTTTATTGGTGATGCAATCCCGCAGGCAATCCTGGAAAAACGCTTgcgcgc                                                                                                                                                                                                                     >  2:406348/2‑251 (MQ=255)                         aCTGGAAAACTAAATGAAACTCTACAATCTGAAAGATCACAATGAGCAGGTCAGCTTTGCGCAAGCCGTAACCCAGGGGTTGGGCAAAAATCAGGGGCTGTTTTTTCCGCACGACCTGCCGGAATTCAGCCTGACTGAAATTGATGAGATGCTGAAGCTGGATTTTGTCACCCGCAGTGCGAAGATCCTCTCGGCGTTTATTGGTGATGAAATCCCGCAGGAAATCCTGGAAGAGCGCGTGCGCGCGGCGt                                                                                                                                                                                                                >  1:433245/1‑251 (MQ=255)                            ggAAAACTAAATGAAACTCTACAATCTGAAAGATCACAATGAGCAGGTCAGCTTTGCGCAAGCCGTAACCCAGGGGTTGGGCAAAAATCAGGGGCTGTTTTTTCCGCACGACCTGCCGGAATTCAGCCTGACTGAAATTGATGAGATGCTGAAGCTGGATTTTGTCACCCGCAGTGCGAAGATCCTCTCGGCGTTTATTGGTGATGAAATCCCGCAGGAAATCCTGGAAGAGCGCGTGCGCGCGGCGttt                                                                                                                                                                                                              <  1:256240/250‑1 (MQ=255)                                aaCTAAATGAAACTCTACAATCTGAAAGATCACAATGAGCAGGTCAGCTTTGCGCAAGCCGTAACCCAGGGGTTGGGCAAAAATCAGGGGCTGTTTTTTCCGCACGACCTGCCGGAATTCAGCCTGACTGAAATTGATGAGATGCTGAAGCTGGATTTTGTCACCCGCAGTGCGAAGATCCTCTCGGCGTTTATTGGTGATGAAATCCCGCAGGAAATCCTGGAAGAGCGCGTGCGCGCGGCGTTTGCCtt                                                                                                                                                                                                         <  1:91782/251‑1 (MQ=255)                                          aaccctACAATCGGAAAGATCACAATGAGCAGGTCAGCTTTGCGCAAGCCGTAACCCAGGGGTTTGGCAAAAATCAGGGGCTGTTTTTTCCGCACGACCTGCCGGAATTCAGCCTGACTGAAATTGATGAGATGCTGAAGCTGGATTTTGTCACCCGCAGTGCGAAGATCCTCTCGGCGTTTATTGGTGATGAAATCCCGCAGGAAATCCTGGAAGAGCGCGTGCGCGCGGCGTTTGCCTTCCCGGCTCCg                                                                                                                                                                                               <  1:383597/247‑1 (MQ=255)                                              ctACAATCTGAAAGATCACAATGAGCAGGTCAGCTTTGCGCAAGCCGTAACCCAGGGGTTGGGCAAAAATCAGGGGCTGTTTTTTCCGCACGACCTGCCGGAATTCAGCCTGACTGAAATTGATGAGATGCTGAAGCTGGATTTTGTCACCCGCAGTGCGAAGATCCTCTCGGCGTTTATTGGTGATGAAATCCCGCAGGAAATCCTGGAAGAGCGCGTGCGCGCGGCGTTTGCCTTCCCGGCTCCGGTCg                                                                                                                                                                                           >  1:187611/1‑251 (MQ=255)                                                            accacaATGAGCAGGCCAGCTTTGCGCTAGCCGTAACCCAGGGGTTGGGCAAAAATCAGGGGCTGTTTTTTCCGCACGACCTGCCGGAATTCAGCCTGACTGAAATTGATGAGATGCTGAAGCTGGATTTTGTCACCCGCAGTGCGAAGATCCTCTCGGCGTTTATTGGTGATGAAATCCCGCAGGAAATCCTGGAAGAGCGCGTGCGCGCGGCGTTTGCCTTCCCGGCTCCGGTCGCCAATGTTGAAAGc                                                                                                                                                                             <  2:328586/249‑1 (MQ=255)                                                               acaATGAGCAGGTCAGCTTTGCGCAAGCCGTAACCCAGGGGTTGGGCAAAAATCAGGGGCTGTTTTTTCCGCACGACCTGCCGGAATTCAGCCTGACTGAAATTGATGAGATGCTGAAGCTGGATTTTGTCACCCGCAGTGCGAAGATCCTCTCGGCGTTTATTGGTGATGAAATCCCGCAGGAAATCCTGGAAGAGCGCGTGCGCGCGGCGTTTGCCTTCCCGGCTCCGGTCGCCAATGTTGAAAGCGAt                                                                                                                                                                          >  1:16663/1‑251 (MQ=255)                                                                caACGAGCAGGTCAGCGTTGCGCAAGCCGTAACCCAGGGGTTGGGCAAAAATCAGGGGCTGTTTTTTCCGCACGACCTGCCGGGATTCCGCCTGACTGAAATTGATGAGATGCTGAAGCTGGATTTTGTCACCCGCAGTGCGAGAATCCTCTCGGCGTTTATTGGTGATGAACTCCCACAGGAAATCCTGGAAACGCGCGTGCGCGCGACGTTTGCCTTCCCGGCTCCGGTCGCCAATGTTgcaggcga                                                                                                                                                                           >  2:190582/1‑242 (MQ=255)                                                                  atgAGCAGGTCATCTTTGCCCAAGCCGTAACCCAGGGGTTGGGCAAAAAGCAGGGGCTGGTGTTTCCGCACGACCTGCCGGAATTCAGCCTGACTGAAATTGATGAGATGCTGAAGCTGGATTTTGGCACCCGCAGTGCGAAGATCCTCTCGGCGTTTATTGGTGATGAAATCCCGCAGGAAATCCTGGAAGAGCGCGTGCGCGCGGCGTTTGCCTTCCCGGCTCCGGTCGCCAATGTTGAAAGCGATGTc                                                                                                                                                                       <  2:59038/249‑1 (MQ=255)                                                                   tgAGCAGGTCAGCTTTGCGCAAGCCGTAACCCAGGGGTTGGGCAAAAATCAGGGGCTGTTTTTTCCGCACGACCTGCCGGAATTCAGCCTGACTGAAATTGATGAGATGCTGAAGCTGGATTTTGTCACCCGCAGTGCGAAGAGCCTCTCGGCGTTTATTGGTGATGAAATCCCGCAGGAAATCCTGGAAGAGCGCGTGCGCGCGGCGTTTGcc                                                                                                                                                                                                           <  1:237039/213‑1 (MQ=255)                                                                             aGCTTTGCGCAAGCCGTAACCCAGGGGTTGGGCAAAAATCAGGGGCTGTTTTTTCCGCACGACCTGCCGGAATTCAGCCTGACTGAAATTGATGAGATGCTGAAGCTGGATTTTGTCACCCGCAGTGCGAAGATCCTCTCGGCGTTTATTGGTGATGAAATCCCGCAGGAAATCCTGGAAGAGCGCGTGCGCGCGGCGTTTGCCTTCCCGGCTCCGGTCGCCAATGTTGAAAGCGATGTCGGTTGTCTgg                                                                                                                                                             >  1:272945/1‑250 (MQ=255)                                                                                     gcAAGCCGTAACCCAGGGGTTGGGCAACAATCAGTGGCTGTTTTTTCCGCACGACCTGCCGGAATTCAGCCTGACTGAAATTGATGAGATGCTGAAGCTGGCTTTTGTCACCCGCAGTGAGAAGGTCCTCTCGGCGTTTATTGGTGATGACATCCCGCAGGAAATCCTGGAACAGCGCGTGCGCGCGGCGTTTGCCTTCCCGGCTCCTGTCGCCAATGTTGAAAGCGATGTCGGTTGTATGGAATTGTTcc                                                                                                                                                    >  2:190854/1‑251 (MQ=255)                                                                                      cAAGCCGTAACCCAGGGGTTGGGCAAAAATCAGGGGCTGTTTTTTCCGCACGACCTGCCGGAATTCAGCCTGACTGAAATTGATGAGATGCTGAAGCTGGATTTTGTCACCCGCAGTGCGAAGATCCTCTCGGCGTTTATTGGTGATGAAATCCCGCAGGAAATCCTGGAAGAGCGCGTGCGCGCGGCGTTTGCCTTCCCGGCTCCGGTCGCCAATGTTGAAAGCGATGTCGGTTGTCTGGAATTGTTcc                                                                                                                                                    >  1:317127/1‑250 (MQ=255)                                                                                       aaGCCGTAACCCAGGGGTTGGGCAAAAAGCAGGGGCTGTTTTTTCCGCACGACCTGCCGGAATTCAGCCTGACTGAAATTGATGAGATGCTGAAGCTGGATTTTGTCACCCGCAGTGCGAAGATCCTCTCGGCGTTTATTGGTGATGAAATCCCGCAGGAAATCCTGGAAGAGCGCGTGCGCGCGGCGTTTGCCTTCCCGGCTCCGGTCGCCAATGTTGAAAGCGATGTCGGTTGTCTGGAATTGTTCCAc                                                                                                                                                  <  1:221367/251‑1 (MQ=255)                                                                                                  cAGGGGTTGGGCAAAAATCAGGGGCTGTTTTTTCCGCACGACCTGCCGGAATTCAGCCTGACTGAAATTGATGAGATGCTGAAGCTGGATTTTGTCACCCGCAGTGCGAAGATCCTCTCGGCGTTTATTGGTGATGAAATCCCGCAGGAAATCCTGGAAGAGCGCGTGCGCGCGGCGTTTGCCTTCCCGGCTCCGGTCGCCAATGTTGAAAGCGATGTCGGTTGTCTGGAATTGTTCCACGGGCCAACGCt                                                                                                                                       <  2:114963/251‑1 (MQ=255)                                                                                                           ggCAAAAATCAGGGGCTGTTTTTTCCGCACGACCTGCCGGAATTCAGCCTGACTGAAATTGATGAGATGCTGAAGCTGGATTTTGTCACCCGCAGTGCGAAGCTCCTCTCGGCGTTTATTGGTGATGAAATCCCGCAGGAAATCCTGGAAGAGCGCGTGCGCGCGGCGTTTGCCTTCCCGGCTCCGGTCGCCAATGTTGAAAGCGATGTCGGTTGTCTGGACTTGTTCCACGGGCCAACGCTGGCATTTa                                                                                                                               >  1:449948/1‑250 (MQ=255)                                                                                                            gCAAAAATCAGGGGCTGTTTTTTCCGCACGACCTGCCGGAATTCAGCCTGACGGAAATTGATGAGATGCTGAAGCTGGATTTTGTCACCCGCAGTTCGAAGATCCTCTCGGCGTTTATTGGTGATGAAATCCCGCAGGAAATCCTGGAAGAGCGCGTGCGCGCGGCGTTTGCCTTCCCGGGTCCGGTCGCCAATGTTGAAAGCGATGTCGGTTGTCTGGAATTGTTCCACGGGCCAACGCTGGCATTTaaa                                                                                                                             <  2:449948/251‑1 (MQ=255)                                                                                                                  aTCAGGGGCTGTTTTTTCCGCACGACCTGCCTGAAGTCAGCCTGACTGAAATTGATGAGATGCTGAAGCTGGATTTTGTCACCCGCAGGGCGAAGATCCTCGCGGCGTTTATTGGTGATGAAACCCCGCAGGAAATCCTGGAAGAGCGCGTGCGCGCGGCGTTTGCCTTCCCGGCTCCGGTCGCCAATGTTGAAATCGATGTCGGTTGTCTTGAATTGTTCCACGGGCCAACGCTGGCATTTAAAGAGTTc                                                                                                                       <  2:316394/251‑1 (MQ=255)                                                                                                                            gtgttttCCGTACGACCTGCCGGAATGCAGCCTGACTGATATGGATGATATGCTGAAGATGGATTTTGTCACCCGCAGTGCGAAGATCCTCTCGTCGTTTATTGGTGATGAAATCCCGCAGGAAATCCTGGAAGATCGCGTGCGCGCGGCGTTTGCCTTCCCGGCTCCGGTCGTCAATGTTGAAAGCGATGTCGGTTGTCTGGAATTGTTCCACGTGCCAACGCTGGCATTTAAAGATTTCGGCGGTCGCt                                                                                                             <  1:12889/248‑1 (MQ=255)                                                                                                                            gTTTTTTCCGCACGACCTGCCGGAATTCAGCCTGACTGAAATTGATGAGATGCTGAAGCTGGATTTTGTCACCCGCAGTGCGAAGATCCTCTCGGCGTTTATTGGTGATGAAATCCCGCAGGAAATCCTGGAAGAGCGCGTGCGCGCGGCGTTTGCCTTCCCGGCTCCGGTCGCCAATGTTGAAAGCGATGTCGGTTGTCTGGAATTGTTCCACGGGCCAACGCTGGCATTTAAAGATTTCGGCGGTCGCt                                                                                                             <  2:187611/251‑1 (MQ=255)                                                                                                                                         gACCTGCCGGAATTCAGCCTGACTGAAATTGATGAGATGCTGAAGCTGGATTTTGTCACCCGCAGTGCGAAGATCCTCTCGGCGTTTATTGGTGATGAAATCCCGCAGGAAATCCTGGAAGAGCGCGTGCGCGCGGCGTTTGCCTTCCCGGCTCCGGTCGCCAATGTTGAAAGCGATGTCGGTTGTCTGGAATTGTTCCACGGGCCAACGCTGGCATTTAAAGATTTCGGCGGTCGCTTTATGGCACAAAt                                                                                                >  1:9145/1‑251 (MQ=255)                                                                                                                                            cTGCCGGAATTCAGCCTGACTGAAATTGATGAGATGCTGAAGCTGGATTTTGTCACCCGCAGTGCGAAGATCCTCTCGGCGTTTATTGGTGATGAAATCCCGCAGGAAATCCTGGAAGAGCGCGTGCGCGCGGCGTTTGCCTTCCCGGCTCCGGTCGCCAATGTTGAAAGCGATGTCGGTTGTCTGGAATTGTTCCACGGGCCAACGCTGGCATTTAAAGATTTCGGCGGGCGCTTTATGGCACAAATGCt                                                                                             >  1:251499/1‑251 (MQ=255)                                                                                                                                                 ggAATTCAGCCTGACTGAAATTGATGAGATGCTGAAGCTGGATTTTGTCACCCGCAGTGCGAAGATCCTCTCGGCGTTTATTGGTGATGAAATCCCGCAGGAAATCCTGGAAGAGCGCGTGCGCGCGGCGTTTGCCTTCCCGGCTCCGGTCGCCAATGTTGAAAGCGATGTCGGTTGTCTGGAATTGTTCCACGGGCCAACGCTGGCATTTACAGATTTCGGCGGTCGCTTTTTGGCGCAAAtgatgaacc                                                                                        >  2:386420/1‑248 (MQ=255)                                                                                                                                                       cAGCCTGACTGAAATTGATGAGATGCTGAAGCTGGATTTTGTCACCCGCAGTGCGAAGATCCTCTCGGCGTTTATTGGTGATGAAATCCCGCAGGAAATCCTGGAAGAGCGCGTGCGCGCGGCGTTTGCCTTCCCGGCTCCGGTCGCCAATGTTGAAAGCGATGTCGGTTGTCTGGAATTGTTCCACGGGCCAACGCTGGCATTTAAAGATTTCGGCGGTCGCTTTATGGCACAAATGCTGACCCATATTg                                                                                  >  1:121494/1‑251 (MQ=255)                                                                                                                                                             gactgaAATTGATGAGATGCTGAAGCTGGATTTTGTCACCCGCAGTGCGAAGATCCCCTCGGCGTTTATTGGTGATGAAATCCCGCAGGAAATCCTGGAAGAGCGCGTGCGCGCGGCGTTTGCCTTCCCGGCTCCGGTCGCCAATGTTGAAAGCGATGTCGGTTGTCTGGAATTGTTCCACGGGCCAACGCTGGCATTTAAAGATTTCGGCGGTCGCTTTATGGCACAAATGCTGACCCATATTGCggg                                                                              <  2:187649/249‑1 (MQ=255)                                                                                                                                                                                 tGAAGCTGGATTTTGTCACCCGCAGTGCGAAGATCCTCTCGGCGTTTATTGGTGATGAAATCCCGCAGGAAATCCTGGAAGAGCGCGTGCGCGCGGCGTTTGCCTTCCCGGCTCCGGTCGCCAATGTTGAAAGCGATGTCGGTTGTCTGGAATTGTTCCACGGGCCAACGCTGGCATTTAAAGATTTCGGCGTTCGCTTTCTAGACCCAATGCTGACCCATATTGCGGGCGAtgatgcagtggtcattctt                                                        >  2:363197/1‑233 (MQ=255)                                                                                                                                                                                            tttGTCACCCGCAGTGCGAAGATCCTCTCGGCGTTTATTGGTGATGAAATCCCGCAGGAAATCCTGGAAGAGCGCGTGCGCGCGGCGTTTGCCTTCCCGGCTCCGGTCGCCAATGTTGAAAGCGATGTCGGTTGTCTGGAATTGTTCCACGGGCCAACGCTGGCATTTAAAGATTTCGGCGGTCGCTTTATGGCACAAATGCTGACCCATATTGCGGGCGATAAGCCAGTGACCATTCTGACCGCGACCt                                              <  1:165296/250‑1 (MQ=255)                                                                                                                                                                                              gggcaaccGCAGTGCGAAGATCCTCGCGGCTGTTATTGGTGATGCAATCCCGCATGAAATCCTGGAAGAGCGCGTGCGCGCGGCGTTTGCCTTCCCGGGTCCGGTCGCCAATGTTGAAAGCGATGTCGGTTGTCTGGAATTGTTCCACGGGCCAACGCTGGCATTTAAAGATTTCGGCGGTCGCTTTATGGCACAAATGCTGACCCATATTGCGGGCGATAAGCCAGTGACCATTCTGACCGCGACCTcg                                            <  2:114657/244‑2 (MQ=255)                                                                                                                                                                                                atccccgcagtgatgatatcttcgtggCGTTTATTGGTTATGAACTCCCGCAGGAAAGCCTGGAAGAGCTCGGTCGCTGGGCGGTAGCGTTACCGGCTCTGGGCGCCAATGTTGAAAGCGAGGTCGGGTGTCGGGGATTGTTCCAGGGGCCAACGCTGGCATTTAAAGATTTCGGCGGTCGCTTTATGGCCCAAATGCTGACCCAAATTTGGGGCGATAAGCAAGTGATCATTCTGATCGCGACATCCGGt                                         <  2:202828/226‑1 (MQ=255)                                                                                                                                                                                                   cccGCAGTGCGAAGATCCTCTCGGCGTTTATTGGTGATGAAATCCCGCAGGAAATCCTGGAAGAGCGCGTGCGCGCGGCGTTTGCCTTCCCGGCTCCGGTCGCCAATGTTGAAAGCGATGTCGGTTGTCTGGAATTGTTCCACGGGCCAACGCTGGCATTTAAAGATTTCGGCGGTCGCTTTATGGCACAAATGCTGAACCATATTGCGGGCGAGAAGCCCGTGACCATTCTGACCGCGACCTCCGGGGAt                                      >  2:393458/1‑251 (MQ=255)                                                                                                                                                                                                     cGCAGTGCGAAGATACTCTCGGCGTTTATTGGTGATAAAATCCCGCAGGAAATCCTGGAAGAGCGCGTGCGCGCGGCGTTTGCCTTCCCGACTCCGGTCGCCAATGTTGAAAGCGATGTCGGTTGTCTGGAATTGTTCCACGGGCCAACGCTGGCATTTAAAGATTTCGGCGGACGCTTTATTGCACAAATGCTGACCCATATTTCGGGCGATTAGCCAGTGACACTACTGACCGCGACCTCCGGTgctcc                                    >  1:293726/1‑247 (MQ=255)                                                                                                                                                                                                                         ggCGTTTATTGGTGATGAAATCCCGCAGGAAATCCTGGAAGAGCGCGTGCGCGCGGCGTTTGCCTTCCCGGCTCCGGTCGCCAATGTTGAAAGCGATGTCGGTTGTCTGGAATTGTTCCACGGGCCAACGCTGGCATTTAAAGATTTCGGCGGTCGCTTTATGGCACAAATGCTGACCCATATTGCGGGCGATAAGCCAGTGACCATTCTGACCGCGACCTC‑GGTGATACCGGAGCGGCAGTGGCTCATGc               <  1:74165/251‑1 (MQ=255)                                                                                                                                                                                                                           ggTTTATTGGTGAGGAAATCCCGCAGGAAATCCTGGAAGAGCGCGTGCGCGCGGCGTTTGCCTTCCCGGCTCCGGTCGCCAATGTTGAAAGCGATGTCGGTTGTCTGGAATTGTTCCACGGGCCAACGCTGGCATTTAAAGATTTCGGCGGTCGCTTTATGGCACAAATGCTGACCCATATTGCGGGCGATAAGCCAGTGACCATTCTGACCGCGACCTCCGGTGATACCGGAGCGGCAGTGGCTCATGCt              <  2:9145/250‑1 (MQ=255)                                                                                                                                                                                                                                   ggTGATGAAATCCCGCAGGAAATCCTGGAAGAGCGCGTGCGCGCGGGGTTTGCCTTCCCGGCTCCGGTCGCCAATGTTGAAAGCGATGTCGGTTGTCTGGAATTGTTCCACGGGCCAACGCTGGCATTTAAAGATTTCGGCGGTCGCTTTATGGCACAAATGCTGACCCATATTGCGGGCGATAAGCCAGTGACCATTCTGACCGCGACCTCCGGTGATACCGGAGCGGCAGTGGCTCATGCTTTCTACgg      <  2:282959/251‑1 (MQ=255)                                                                                                                                                                                                                                       atgaAATCCCGCAGGAAATCCTGGAAGAGCGCGTGCGCGCGGCGTTTGCCTTCCCGGCTCCGGTCGCCAATGTTGAAAGCGATGTCGGTTGTCTGGAATTGTTCCACGGGCCAACGCTGGCATTTAAAGATTTCGGCGGTCGCTTTATGGCACAAATGCTGACCCATATTGCGGGCGATAAACCAGTGACCATTCTGACCGCAACCTCCGGTGATACCGGAGCGGCAGTGGCTCATGCTTTCTACGGtttt  >  1:415541/1‑250 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                 GCTGGATACGGCGGGCGCACGAGTACTGGAAAACTAAATGAAACTCTACAATCTGAAAGATCACAACGAGCAGGTCAGCTTTGCGCAAGCCGTAACCCAGGGGTTGGGCAAAAATCAGGGGCTGTTTTTTCCGCACGACCTGCCGGAATTCAGCCTGACTGAAATTGATGAGATGCTGAAGCTGGATTTTGTCACCCGCAGTGCGAAGATCCTCTCGGCGTTTATTGGTGATGAAATCCCACAGGAAATCCTGGAAGAGCGCGTGCGCGCGGCGTTTGCCTTCCCGGCTCCGGTCGCCAATGTTGAAAGCGATGTCGGTTGTCTGGAATTGTTCCACGGGCCAACGCTGGCATTTAAAGATTTCGGCGGTCGCTTTATGGCACAAATGCTGACCCATATTGCGGGTGATAAGCCAGTGACCATTCTGACCGCGACCTCCGGTGATACCGGAGCGGCAGTGGCTCATGCTTTCTACGGTTTA  >  NC_000913/3697‑4177

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑