Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,440,768	G→A	L150L (CTG→TTG)	paeA ←	putative cadaverine/putrescine exporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,440,768	0	G	A	100.0%	163.0 / NA	50	L150L (CTG→TTG)	paeA	putative cadaverine/putrescine exporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (21/29);  total (21/29)

GCTCATCTTCGTGGAGATCAAACCAAATCACGTTTTCACGCGGTGTCATTGAAGACGGAACGGTACGGGATTCCAGCTCAAAGACGTTTTCAATCAGCTCGTGTTCCTGTTTACGTAACACGCCCGCCAGCGCACCGGCTTCCACTACCGCGTAGATGTCATCAGAAGTGATGTCATCTTTACGTACCATTGGCAGTTTGAAAATACGGAAGATTATGTTCGCCAGGCCGTTGAAGAACCACACCAGCGGGGTGCAAACGTACAGGCAGAAGCGCATCGGGTTGATGATACGCAAAGCCACAGCTTCTGGCGCAATCATACCGATGCGTTTCGGGGTTAAATCCGCAAACAGGATAAACATGCCAGTCACTAACGAGAAAGAGAGAATAAAGCTCAGTTGCTCAGAGAGCTCTGCCGACATATAGCGGGAGAACAGGCTGTGAAAAGCTGGAGAAAATGCCGCATCACCGACGATACCGCCGAGA  >  NC_000913/4440522‑4441006                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                               gCTCATCTCCGTGGAGATCAAACCAAATCACGTTTTCACGCGGTGTCTTTGAAGACGGAACGGTACGGGATTCCAGCTCAAAGACGTTTTCAATCAGCTCGTGTTCCTGTTTACGTAACACGCCCGCCAGCGCACCGGCTTCCACTACCGCGTAGATGTCATCAGAAGTGATGTCATCTTTACGTACCATTGGCAGTTTGAAAATACGGAAGATTATGTTCGCCCGGCCGTTGTAGAACCACACCAACgg                                                                                                                                                                                                                                             <  1:314925/250‑1 (MQ=255)          cggggaaaaaaaaccaaaccCCTTTTTCCCGCGGTGTCATTGAAGACGGACCGTAACGGTATTCCAGCTCAAAACCGTTTTCAATCACCCCGTTTTCCTGTTTACGTAACACGCCCGCCAGCGCACCGGCTTCCACTACCGCGTAGATGTCATCAGAAGTGATGTCATCTTTACGTACCATTGGCAGTTTGAAAATACGGAAGATTATGTTCGCCAGGCCGTTGAAGAACCACACCAACGGGGTGCAAAc                                                                                                                                                                                                                                    <  2:238064/240‑1 (MQ=255)           ggggAGATCAACCCAAATCACGTTTTCACGCGGTGTCATTGAAGACGGACCGGTACGGGATTCCAGCTCAAATCCGTTTTCAATCAGCTCGTGTTCCTGTTTACGTAACACGCCCGCCAGCGCACCGGCTTCCACTACCGCGTAGATGTCATCAGAAGTGATGTCATCTTTACGTACCATTGGCAGTTTGAAAATACGGAAGATTATGTTCGCCAGGCCGTTGAAGAACCACACCAACGGGGTGCAAACGt                                                                                                                                                                                                                                  <  2:386010/249‑1 (MQ=255)                aaTCAAACCAAATCACGTTTTCACGCGGTGTCATTGAAGACGGAACGGTACGGGATTCCAGCTCAAAGACGTTTTCAATCAGCTCGTGTTCCTGTTTACGTAACACGCCCGCCAGCGCACCGGCTTCCACTACCGCGTAGATGTCATCAGAAGTGATGTCATCTTTACGTACCATTGGCAGTTTGAAAATACGGAAGATTATGTTCGCCAGGCCGTTGAAGAACCACACCAACGGGGTGCAAACGTACAgg                                                                                                                                                                                                                             <  1:463680/250‑1 (MQ=255)                           atcccGTTTGCACGCGGTGTCATTGAAGACGGAACGGTAGGGGATTCCAGCTCAAAGACGTTTTCAATCAGCCCGTGTTCCTGTTTACGTAACACGCCCGCCAGCGCACCGGCTTCCACTACCGCGTAGATGTCATCAGAAGTGATGTCATCTTTACGTACCATTGGCAGTTTGAAAATACGGAAGATTATGTTCGCCAGGCCGTTGAAGAACCACACCAACGGGGTGCAAACGTACAGGCAGAAGCGCAt                                                                                                                                                                                                                  <  1:351936/247‑1 (MQ=255)                               cGTTTTCACGCGGTGTCATTGAAGACGGAACGGTACGGGATTCCAGCTCAAAGACGTTTTCAATCAGCTCGTGTTCCTGTTTACGTAACACGCCCGCCAGCGCACCGGCTTCCACTACCGCGTAGATGTCATCAGAAGTGATGTCATCTTTACGTACCATTGGCAGTTTGAAAATACGGAAGATTATGTTCGCCAGGCCGTTGAAGAACCACACCAACGGGGTGCAAACGTACAGGCAGAAGCGCATCggg                                                                                                                                                                                                              >  2:180247/1‑251 (MQ=255)                                gTTTTCACGCGGTGTCATTGAAGACGGAACGGAACGGGATTACAGCTCAAAGGTGTGTTCAATCAGCTCGTGTTCCTGGTTACGTAACACGACCGCAAGCGCACCGGCTTCCACTACCGCGTAGATGTCATCAGAAGAGGTGTGATCTTTACGTACCATTGGCAGTTTGGAAATATGGAAGATTATGGTCGCCAGGACATTGAAGACCAACACCAACGGGGTGCAAACGTACAGGCAGaaactcatctggt                                                                                                                                                                                                             >  2:217937/1‑240 (MQ=255)                                   ttCACGCGGTGTCATTGAAGACGGAACGGTACGGGATTCCAGCTCAAAGACGTTTTCAATCAGCTCGTGTTCCTGTTTACGTAACACGCCCGCCAGCGCACCGGCTTCCACTACCGCGTAGATGTCATCAGAAGTGATGTCATCTTTACGTACCATTGGCAGTTTGAAAATACGGAAGATTATGTTCGCCAGGCCGTTGAAGAACCACACCAACGGGGTGCAAACGTACAGGCAGAAGCGCATCGGGTtg                                                                                                                                                                                                           >  1:132976/1‑250 (MQ=255)                                           gtgtCATTGAAGACGGAACGGTGCGGGATTCCAGCTCAAAGACGTTTTCAATCAGCTCGTGTTCCTGTTTACGTAACACGCCCGCCAGCGCACCGGCTTCCACTACCGCGTAGATGTCATCAGAAGTGATGTCATCTTTACGTACCATTGGCAGTTTGAAAATACGGAAGATTATGTTCGCCAGGCCGTTGAAGAACCACACCAACGGGGTGCAAACGTACAGGCAGAAGCGCATCGGGTTGATGATACGc                                                                                                                                                                                                  >  1:294300/1‑251 (MQ=255)                                            tgtCATTGAAGACGGAACGGTACGGGATTCCAGCTCACAGACGTTTTCAATCAGCTCGTGTTCCTGTTTACGTAACACGCCCGCCAGCGCACCGGCTTCCACTACCGCGTAGATGTCATCAGAAGTGATGTCATCTTTACGTACCATTGGCAGTTTGAAAATACGGAAGATTATGTTCGCCAGGCCGTTGAAGAACCACACCAACGGGGTGCCAACGTACAGGCAGAAGCGCATCGGGTTGATGATACGc                                                                                                                                                                                                  >  1:138635/1‑250 (MQ=255)                                             gtCATTGAAGACGGGACGGTACGGGATTCCAGCTCAAAGACGTTTTCAATCAGCTCGTGTTCCTGTTTACGTAACACGCCCGCCAGCGCACCGGCTTCCACTACCGCGTAGATGTCATCAGAAGTGATGTCATCTTTACGTACCATTGGCAGTTTGAAAATACGGAAGATTATGTTCGCCAGGCCGTTGAAGAACCACACCAACGGGGTGCAAACGTACAGGCAGACACGCATCGGGTTGATGATACGCa                                                                                                                                                                                                 >  2:86527/1‑250 (MQ=255)                                                             cGGTACGGGATTCCAGCTCAAAGACGTTTTCAATCAGCTCGTGTTCCTGTTTACGTAACACGCCCGCCAGCGCACCGGCTTCCACTACCGCGTAGATGTCATCAGAAGTGATGTCATCTTTACGTACCATTGGCAGTTTGAAAATACGGAAGATTATGTTCGCCAGGCCGTTGAAGAACCACACCAACGGGGt                                                                                                                                                                                                                                          >  1:68009/1‑193 (MQ=255)                                                             cGGTACGGGATTCCAGCCCAAAGACGTTTTCAATCAGCCCGTGTCCCTGTTTACGTAACACGCCCGCCAGCGCACCGGCTTCCACTACCGCGTAGATGTCATCAGAAGTGATGTCATCTTTACGTACCATTGGCAGTTTGAAAATACGGAAGATTATGTTCGCCAGGCCGTTGAAGAACCACACCAACGGGGt                                                                                                                                                                                                                                          <  2:68009/193‑1 (MQ=255)                                                                     gatcccAGCTAAAAGACGTTTTCAATCAGCTCGTGTCCCTTTTTCCGTAACACCCCCGCCAGCGCACCGGCGTCCACTACCGCGTAGATGTCATCAGAAGTGATGTCATCTTTACGTACCATTGGCAGTTTGAAAATACGGAAGATTATGTTCGCCAGGCCGTTGAAGAACCACACCAACGGGGTGCAAACGTACAGGCAGAAGCGCATCGGGTTGATGATACGCAAAGCCACAGCTTCTGGCGCAATCAt                                                                                                                                                                        <  2:395309/247‑1 (MQ=255)                                                                        tCCAGCTCAAAGTCTTTTTCAATCATCTCGTTTTCCTGTTTACGTAACACGCCCGCCAGCGCACCGGCTTCCACTACCGCGTAGATGTCATCAGAAGTGATGTCATCTTTACGTACCATTGGCAGTTTGAAAATACGGAAGATTATGTTCGCCAGGCCGTTGAAGAACCACACCAACGGGGTGCAAACGTACAGGCAGAAGCGCATCGGGTTGATGATACGCAAAGCCACAGCTTCTGGCGCAATCATAc                                                                                                                                                                      <  1:426608/250‑1 (MQ=255)                                                                        tCCAGCTCAAAGACGTTTTCAATCAGCTCGTTTTCCTGTTTACGTAACACGCCCGCCAGCGCACCGGCTTCCACTACCGCGTAGATGTCATCAGAAGTGATGTCATCTTTACGTACCATTGGCAGTTTGAAAATACGGAAGATTATGTTCGCCAGGCCGTTGAAGAACCACACCAACGGGGTGCAAACGTACAGGCAGAAGCGCATCGGGTTGATGATACGCAAAGCCACAGCTTCTGGCGCAATCATAc                                                                                                                                                                      <  1:224587/250‑1 (MQ=255)                                                                            gCTCAAAGACGTTTTCAATCAGCTCGTGTTCCGGTTTACGAAACACGCCCGCCAGCGCACCGGCTTCCACTACCGCGTAGATGTCATCAGAAGTGATGTCATCTTTACGTACCATTGGCAGTTTGAAAATACGGAAGATTATGTTCGCCAGGCCGTTGAAGAACCACACCAACGGGGTGCAAACGTACAGGCAGAAGCGCATCGGGTTGATGATACGCAAAGCCACAGCTTCTGGCGCAATCATACCGATg                                                                                                                                                                 <  1:173254/251‑1 (MQ=255)                                                                                  aGACGTTTTCAATCAGCTCGTGTTCCTGTTTACGTAACACGCCCGCCAGCGCACCGGCTTCCACTACCGCGTAGATGTCATCAGAAGTGATGTCATCTTTACGTACCATTGGCAGTTTGAAAATACGGAAGATTATGTTCGCCAGGCCGTTGAAGAACCACACCAACggg                                                                                                                                                                                                                                            >  1:54383/1‑170 (MQ=255)                                                                                  aGACGTTTTCAATCAGCTCGTGTTCCTGTTTACGTAACACGCCCGCCAGCGCACCGGCTTCCACTACCGCGTAGATGTCATCAGAAGTGATGTCATCTTTACGTACCATTGGCAGTTTGAAAATACGGAAGATTATGTTCGCCAGGCCGTTGAAGAACCACACCAACGGGGTGCAAACGTACAGGCAGAAGCGCATCGGGTTGATGATACGCAAAGCCACAGCTTCTGGCGCAATCATACCGATGCGTTTc                                                                                                                                                           <  1:115843/251‑1 (MQ=255)                                                                                  aGACGTTTTCAATCAGCTCGTGTCCCGGTTTACTTAACACGCCCGCCAGCGCACCGGCTTCCACTACCGCGTAGATGTCATCAGAAGTGATGTCATCTTTACGTACCATTGGCAGTTTGAAAAGACGGAAGATTATGTTCGCCAGGCCGTTGAAGAACCACACCAACggg                                                                                                                                                                                                                                            <  2:54383/170‑1 (MQ=255)                                                                                  aGACGTTTTCAACCAGCTCGTGTTCCTGTTAACGTAACACGCCCGCCAGCGCACCGGCTTCCACTACCGCGTAGATGTCATCAGAAGTGATGTCATCTTTACGTACCATTGGCAGTTTGAAAATACGGAAGATTATGTTCGCCAGGCCGTTGAAGAACCACACCAACGGGGTGCAAACGTACAGGCAGAAGCGCATCGGGTTGATGATACGCAAAGCCACAGCTTCTGGCGCAATCATACCGATGCGttgc                                                                                                                                                           <  1:115832/251‑3 (MQ=255)                                                                                      gTTTTCAATCAGCTCGTGTTCCTGTTTACGTACCACCCCCGCCAGCGCACCTGCTTCCACTACCGCGTAGATGTCATCAGAAGTGATGTCAGCTTTACGTACCATTGGCAGTTTGAAAATACGGAAGATTATGTTCGCCAGGCCGTTGAAGAACCACACCAACGGGGTGCAAACGTACAGGCAGAAGCGCATCGGGTTGATGATACGCAATGCCACAGCTTCTGGCGCAATCATACCGATGCGTTTCgggg                                                                                                                                                       <  1:40605/251‑1 (MQ=255)                                                                                                    cGTGTTCCTGTTTACGTAACACGCCCGCCAGCGCACCGGCTTCCACTACCGCGTAGATGTCATCAGAAGTGATGTCATCTTTACGTACCATTGGCAGTTTGAAAATACGGAAGATTATGTTCGCCAGGCCGTTGAAGAACCACACCAACGGGGTGCAAACGTACAGGCAGAAGCGCATCGGGTTGATGATACGCAAAGCCACAGCTTCTGGCGCAATCATACCGATGCGTTTCGGGGTTAAATCCGCAAAc                                                                                                                                         >  1:13599/1‑251 (MQ=255)                                                                                                      tgtTCCTGTTTACGTAACACGCCCGCCAGCGCACCGGCTTCCACTACCGCGTAGATGTCATCAGAAGTGATGTCATCTTTACGTACCATTGGCAGTTTGAAAATACGGAAGATTATGTTCGCCAGGCCGTTGAAGAACCACACCAACGGGGTGCAAACGTACAGGCAGACGCGCATCGGGTTGGTGATACGCAAAGCCACAGCTTCTGGCGCAATCATACCGATGCGTTTCGGGGTTAAATCCGCaacc                                                                                                                                         >  2:115738/1‑247 (MQ=255)                                                                                                            ggtttaattAACCCGCCCGCCAGCGCACCGGCTTCCACTACCGCGTAGATGTCATCAGAAGTGATGTCATCTTTACGTACCATTGGCAGTTTGAAAATACGGAAGATTATGTTCGCCAGGCCGTTGAAGAACCACACCAACGGGGTGCAAACGTACAGGCAGAAGCGCATCGGGTTGATGATACGCAAAGCCACAGCTTCTGGCGCAATCATACCGATGCGTTTCGGGGTTAAATCCGCAAACAGGAt                                                                                                                                    <  2:287660/240‑1 (MQ=255)                                                                                                                tacctAACACCCCCGCCAGCGCACCGGCTTCCACTACCGCGTAGATTTCATCAAGAGTGATGTCATCTTTACGTTCCATTGGCAATTTGAAAATACGGAAAATTATGTTCGCCAGGCCGTTGAAGAACCACACCAACGGGGTGCAAACGTACAGGCAGAAGCGCATCGGGTTGATGATACGCAGAGCCACAGCTTCTGGCGCAATCATACCGATGCGTTTCGGGGTTAAATCCGCAAACAGGATAAACAt                                                                                                                              <  1:384976/246‑1 (MQ=255)                                                                                                                 ccGTAACACGCCCGCCAGCGCACCGTCTTCCACTACCGCGTAGATGTCATCAGAAGTGATGTCATCTTTACGTACCATTGGCAGTGTGAAAAGACGGAAGATTATGTTCGCCAGGCCGTTGAAGAACCACACCAACGGGGTGCAAACTTACAGGCAGAAGTGCATCGGGTTGATGATACGCAAAGCCACAGCTTCTGGCGCAATCATACCGATGCGTTTCGGGGTTAAATCCGCAAACAGGATAAACATGc                                                                                                                            <  1:316779/250‑1 (MQ=255)                                                                                                                     aacccgcccgccAGCGCACCGGCTTCCACTACCGCGTAGATGTCATCAGAAGTGATGTCATCTTTACGTACCATTGGCAGTTTGAAAATACGGAAGATTATGTTCGCCAGGCCGTTGAAGAACCACACCAACGGGGTGCAAACGTACAGGCAGAAGCGCATCGGGTTGATGATACGCAAAGCCACAGCTTCTGGCGCAATCATACCGATGCGTTTCGGGGTTAAATCCGCAAACAGGATAAACATGCCAGt                                                                                                                        <  1:169458/247‑1 (MQ=255)                                                                                                                                                aCTACCGCGTAGATGTCATCAGAAGTGATGTCATCTTTACGTACCATTGGCAGTTTGAAAATACGGAAGATTATGTTCGCCAGGCCGTTGAAGAACCACACCAACGGGGTGCAAACGTACAGGCAGAAGCGCATCGGGTTGATGATACGCAAAGCCACAGCTTCTGGCGCAATCATACCGATGCGTTTCGGGGTTAAATCCGCAAACAGGATAAACATGCCAGTCACTAACGAGAAAGAGAGAATAAAGCt                                                                                             <  1:180247/251‑1 (MQ=255)                                                                                                                                                    ccGCGTAGATGTCATCAGAAGTGATGTCATCTTTACGTACCATTGGCAGTTTGAAAATACGGAAGATTATGTTCGCCAGGCCGTTGAAGAACCACACCAACGGGGTGCAAACGTACAGGCAGAAGCGCATCGGGTTGATGATACGCAAAGCCACAGCTTCTGGCGCAATCATACCGATGCGTTTCGGGGTTAAATCCGCAAACAGGATAAACATGCCAGTCACTAACGAGAAAGAGAGAATAAAGCTCAGt                                                                                         >  1:235720/1‑251 (MQ=255)                                                                                                                                                          aGATGTCATCAGAAGTGATGTCATCTTTACGTACCATTGGCAGTTTGAAAATACTGAAGATTATGTTCGCCAGGCCGTTGAAGAACCACACCAACTGGGTGCAAACGTACAGGCAGAAGCGCATCGGGTTGATGATACGCAAAGCCACAGCTTCTGGCGCAATCATACCGATGCGTTTCGGGGTTAAATCCGCAAACAGGATAAACATGCCAGGCACTAACGAGAAAGAGAGAATAAAGCTCAGTTGCTCa                                                                                   <  2:405181/251‑1 (MQ=255)                                                                                                                                                           gATGTCATCAGAAGTGATGTCATCTTTACGTACCATTGGCAGTTTGAAAATACGGAAGATTATGTTCGCCAGGCCGTTGAAGAACCACACCAACGGGGTGCAAACGTACAGGCAGAAGCGCATCGGGTTGATGATACGCAAAGCCACAGCTTCTGGCGCAATCATACCGATGCGTTTCGGGGTTAAATCCGCAAACAGGATAAACATGCCAGTCACTAACGAGAAAGAGAGAATAAAGCTCAGTTGCTc                                                                                    >  1:467234/1‑249 (MQ=255)                                                                                                                                                            aTGTCATCAGAAGTGATGTCATCTTTACGTACCATTGGCAGTTTGAAAATACGGAAGATTATGTTCGCCAGGCCGTTGAAGAACCACACCAACGGGGTGCAAACGTACAGGCAGAAGCGCATCGGGTTGATGATACGCAAAGCCACAGCTTCt                                                                                                                                                                                   >  1:183606/1‑153 (MQ=255)                                                                                                                                                            aTGTCATCAGAAGTGATGTCATCTTTACGTACCATTGGCAGTTTGAAAATACGGAAGATTATGTTCGCCAGGCCGTTGAAGAACCACACCAACGGGGTGCAAACGTACAGGCAGAAGCGCATCGGGTTGATGATACGCAAAGCCACAGCTTCt                                                                                                                                                                                   <  2:183606/153‑1 (MQ=255)                                                                                                                                                               tcatcaGAAGTGATGTCATCTTTACGTACCATTGGCAGTTTGAAAATACGGAAGATTATGTTCGCCAGGCCGTTGAAGAACCACACCAACGGGGTGCAAACGTACAGGCAGAAGCGCATCGGGTTGATGATACGCAAAGCCACAGCTTCTGGCGCAATCATACCGATGCGTTTCGGGGTTAAATCCGCAAACAGGATAAACAt                                                                                                                              <  2:405434/203‑1 (MQ=255)                                                                                                                                                               tcatcaGAAGTGATGTCATCTTTACGTACCATTGGCAGTTTGAAAATACGGAAGATTATGTTCGCCAGGCCGTTGAAGAACCACACCAACGGGGTGCAAACGTACAGGCAGAAGCGCATCGGGTTGATGATACGCAAAGCCACAGCTTCTGGCGCAATCATACCGATGCGTTTCGGGGTTAAATCCGCAAACAGGATAAACAt                                                                                                                              >  1:405434/1‑203 (MQ=255)                                                                                                                                                                          gATGTCATCTTTACGTACCATTGGCAGTTTGAAAATACGGAAGATTATGTTCGCCAGGCCGTTGAAGAACCACACCAACGGGGTGCAAACGTACAGGCAGAAGCGCATCGGGTTGATGATACGCAAAGCCACAGCTTCTGGCGCAATCATACCGATGCGTTTCGGGGTTAAATCCGCAAACAGGATAAACATGCCAGTCACTAACGAGAAAGAGAGAATAAAGCTCAGTTGCTCAGAGAGCTCTGCCGAc                                                                    >  2:172580/1‑250 (MQ=255)                                                                                                                                                                             gTCATCTTTACGTACCATTGGCATTTTGAAAATACGGAAGATTATGTTCGCCAGGCCGTTGAAGAACCACACCAACGGGGTGCAAACGTACAGGCAGAAGCGCATCGGGTTGATGATACGCAAAGCCACAGCTTCTGGCGCAATCATACCGATGCGTTTGGGGGTTAAAGCCGCAAACAGGAGAAACATGCCAGTCACTAACGAGAAAGAGAGAATAAAGCTCAGTTGCTCAGAGAGCTCTGCCGACatat                                                                <  1:28848/251‑1 (MQ=255)                                                                                                                                                                              tCATCTTTACGTACCATTGGCAGTTTGAAAATACGGACGATTATGTGCGCCCAGCCGTTGAAGAACCACACCAACGGGGTGCAAACGTACAGGCAGAAGCGCATCGGGTTGATGCTCCGCAAAGCCACAGCTTCTGGCGCAATCATACCGCTGCGTTTCGGGGATAAGTCCGCAAACAGGATAAACATTCCAGTCCCTAACGAGCAAGAGAGAATACAGCTCAGTTGATCAGAGAGCTCAGCCGCCatat                                                                >  2:45201/1‑250 (MQ=255)                                                                                                                                                                                  cTTTACGTACCATTGGCAGTTTGAAAATACGGAAGATTATGTTCGCCAGGCCGTTGAAGAACCACACCCACGGGGTGCAAACGTACAGGAAGAAGCGCATCGGGCTGATGATACGCAAAGCCACAGCTTCTGGCGCAATCATACCGATGCGTTTCGGGGTTAAATCCGCAAACAGGATAAACATGCCAGTCACTAACGAGAAAGAGAGAATAAAGCTCAGTTGCTCAGAGAGCTCTGCCGACATATAGCgg                                                           >  1:245301/1‑251 (MQ=255)                                                                                                                                                                                        gTACCATTGGCAGTTTGAAAATACGGAAGATTATGTTCGCCAGGCCGTTGAAGAACCACACCAACGGGGTGCAAACGTACAGGCAGAAGCGCATCGGGTTGATGATACGCAAAGCCACAGCTTCTGGCGCAATCATACCGATGCGTTTCGGGGTTAAATCCGCAAACAGGATAAACATGCCAGTCACTAACGAGAAAGAGAGAATAAAGCTCAGTTGCTCAGAGAGCTCTGCCGACATATAGCGGGAGAAc                                                     >  1:315990/1‑251 (MQ=255)                                                                                                                                                                                         tACCATTGGCAGTTTGAAAATACGGAAGATTATGTTCGCCAGGCCGTTGAAGAACCACACCAACGGGGTGCAAACGTACAGGCAGAAGCGCATCGGGTTGATGATACGCAAAGCCACAGCTTCTGGCGCAATCATACCGATGCGTTTCGGGGTTAAATcc                                                                                                                                               >  1:475428/1‑160 (MQ=255)                                                                                                                                                                                         tACCATTGGCAGTTTGAAAATACGGAAGATTATGTTCGCCAGGCCGTTGAAGAACCACACCAACGGGGTGCAAACGTACAGGCAGAAGCGCATCGGGTTGATGATACGCAAAGCCACAGCTTCTGGCGCAATCATACCGATGCGTTTCGGGGTTAAATcc                                                                                                                                               <  2:475428/160‑1 (MQ=255)                                                                                                                                                                                          aCCATTGGCAGTTTGAAAATACGGAAGATTATGTTCGCCAGGCCGTTGAAGAACCACACCAACGGGGTGCAAACGTACAGGCAGAAGCGCATCGGGTTGATGATACGCAAAGCCACAGCTTCTGGCGCAATCATACCGATGCGTTTCGGGGTTAAATCCGCAAACAGGATAAACATGCCAGTCACTAACGAGAAAGAGAGAATAAAGCTCAGTTGCTCAGAGAGCTCTGCCGACATATAGCGGGAGAACAg                                                   <  1:172580/251‑1 (MQ=255)                                                                                                                                                                                            catgggCAGTGTTAAAAAACGGAAGATTATGTTCGCCAGGCCGTTGAAGAACCACACCAAAGGGGTGCAAACGTACAGGCAGAAGCGCATCGGGTTGATGATACGCAAAGCCACAGCTTCTGGCGCAATCATACCGATGCGTTTCGGGGTTAAATCCGCAAACAGGATAAACATGCCAGTCACTAACGAGAAAGAGAGAATAAAGCTCAGTTGCTCAGAGAGCTCTGCCGACATAGAGCGGGAGAACAGGc                                                 <  1:105581/247‑1 (MQ=255)                                                                                                                                                                                                   aGTTTGAAAATACGGAAGATTATGCTCGCCAGGCCGTTGAAGAACCACACCAACGGGGTGCAAACGTACAGGCAGAAGCGCATCGGGTTGATGATACGCAAAGCCACAGCTTCTGGCGCAATCATACCGATGCGTTTCGGGGTTAAATCCGCAAACAGGATAAACATGCCAGTCACTAACGAGAAAGAGAGAATAAAGCTCAGTTGCTCAGAGAGCTCTGCCGACATATAGCGGGAGAACAGGCTGTGaaa                                          <  1:260270/251‑1 (MQ=255)                                                                                                                                                                                                              aCGGAAGATTATGTTCGCCAGGCCGTTGAAGAACCACACCAACGGGGTGCAAACGTACAGGCAGAAGCGCATCGGGTTGATGATACGCAAAGCCACAGCTTCTGGCGCAATCATACCGATGCGTTTCGGGGTTAAATCCGCAAACAGGATAAACATGCCAGGCACTAACGAGAAAGAGAGAATAAAGCTCAGTTGCTCAGAGAGCTCTGCCGACATATAGCGGGAGAACAGGCTGTGAAAAGCTGGAGaa                                <  1:45201/250‑1 (MQ=255)                                                                                                                                                                                                                               ccAGGCCGTTGAAGAACCACACCAACGGGGTGCAAACGTACAGGCAGAAGCGCATCGGGTTGATGATACGCAAAGCCACAGCTTCTGGCGCAATCATACCGATGCGTTTCGGGGTTAAATCCGCAAACAGGATAAACATGCCAGTCACTAACGAGAAAGAGAGAATAAAGCTCAGTTGCTCAGAGAGCTCTGCCGACATATAGCGGGAGAACAGGCTGTGAAAAGCTGGAGAAAATGCCGCATCACcgacg              >  2:140829/1‑251 (MQ=255)                                                                                                                                                                                                                                           agaaCCACACCAACGGGGTGCAAACGTACAGGCAGAAGCGCATCGGGTTGATGATACGCAAAGCCACAGCTTCTGGCGCAATCATACCGATGCGTTTCGGGGTTAAATCCGCAAACAGGATAAACATGCCAGTCACTAACGAGAAAGAGAGAATAAAGCTCAGTTGCTCAGAGAGCTCTGCCGACATATAGCGGGAGAACAGGCTGTGAAAAGCTGGAGAAAATGCCGCATCACCGACGATACCGCCgaga  <  1:332227/251‑1 (MQ=255)                                                                                                                                                                                                                                           agaaCCACACCAACGGGGAGCAAACGTACAGGCAGAAGCGCATCGGGTTGATGATACGAAAAGCCACAGCTTCTGGCGCAATCATACCGATGCGTTTCGGGGTTAAATCCGCAAACAGGATAAACATGCCAGTCACTAACGAGAAAGAGAGAATAAAGCTCAGTTGCTCAGAGAGCTCTGCCGACATAAAGCGGGAGAACAGGCTGAGAAAGGCTGGAGAAAATGCAGCATCACCGCCGATAccacag     >  2:430082/1‑244 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                               GCTCATCTTCGTGGAGATCAAACCAAATCACGTTTTCACGCGGTGTCATTGAAGACGGAACGGTACGGGATTCCAGCTCAAAGACGTTTTCAATCAGCTCGTGTTCCTGTTTACGTAACACGCCCGCCAGCGCACCGGCTTCCACTACCGCGTAGATGTCATCAGAAGTGATGTCATCTTTACGTACCATTGGCAGTTTGAAAATACGGAAGATTATGTTCGCCAGGCCGTTGAAGAACCACACCAGCGGGGTGCAAACGTACAGGCAGAAGCGCATCGGGTTGATGATACGCAAAGCCACAGCTTCTGGCGCAATCATACCGATGCGTTTCGGGGTTAAATCCGCAAACAGGATAAACATGCCAGTCACTAACGAGAAAGAGAGAATAAAGCTCAGTTGCTCAGAGAGCTCTGCCGACATATAGCGGGAGAACAGGCTGTGAAAAGCTGGAGAAAATGCCGCATCACCGACGATACCGCCGAGA  >  NC_000913/4440522‑4441006

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑