Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,437,890	C→T	Q62* (CAG→TAG)	ytfI →	protein YtfI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,437,890	0	C	T	100.0%	56.2 / NA	19	Q62* (CAG→TAG)	ytfI	protein YtfI
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (10/9);  total (10/9)

GAGATATTTAATGGCGACATAAAGTAACCAAATAAAATAAGGTTGTCATATGTTACCCAGGATCAGACACAATAATTTTATTGGTGCGGTGGAGTTATTTGTAAAGTCTTCGTATACAAAAACACATTCAAACAATTTTTTTAACAACATCCATCATGCATTTAAGAAAAAAGATTGGATTTCGAATTATGATAGCCTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTCAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGTGCCGAATATACAATGACATTGCGAGGTAGTGGATTTGAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACG  >  NC_000913/4437658‑4438113                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                gagaTATTTAATGGCGACATAAAGTAAC‑AAATAAAATAAGGTTGTCATATGTTACCCAGGATCAGACACAATAATTTTATTGGTGCGGTGGAGTTATTTGTAAAGTCTTCGTATACAAAAACACATTCAACCAATTTTTTTAACAACATCCAGCATGCATTTAAGAAAAAAGATTGGATTTCGAATTATGATAGCCTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTTAGATTGATAAAAGTGGTTa                                                                                                                                                                                                              <  1:363457/251‑1 (MQ=255)                  cataaatgaaacAAATAAAATAAGGTTTGCATATGTTACCCAGGATCAGACACAATAATTTTATTGGTGCGGTGGATTTATTTGTAAAGTCTTCGTATACAAAAACACATTCAAACAATTTTTTTAACAACATCCATCATGCATTTAAGAAAAAAGATTGGATTTCGAATTATGATAGCCTGTTAACTTTGAGGGAGTTCTTGCGTTGCGCAACTTAGATTGATAAAAGTGGTTATCAGGTGCTTtcatca                                                                                                                                                                                              <  2:449602/240‑1 (MQ=255)                     aaaGTAACCAAATAAAATAAGGTTGTCATATGTTACCCAGGATCAGACACAATAATTTTATTGGTGCGGTGGAGTTATTTGTAAAGTCTTCGTATACAAAAACACATTCAAACAATTTTTTTAACAACATCCATCATGCATTTAAGAAAAAAGATTGGATTTCGAATTATGATAGCCTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTTAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCaaa                                                                                                                                                                                            <  1:103963/250‑1 (MQ=255)                                                        cccAGGATCATACACAATAATTTTTTTGGTGCGGTGGAGTTATTTGTTCAGTTTTCGTATACAAAAACACATTCAAACAAATTTTTTAACAACATCCATCATGCATTTAAGAAAAAAGATTTGATTTCGAATTATGATAGCCTGTTATCTTTGAGGGAGTTCTTTCGTTGCGCAACTTAGATTGATAAAAGTGGTTAACAGGTGCTTTCATCAAAAAATGAAACTGTCCACGCCATGGTTAAGTTTCttt                                                                                                                                                         <  2:345188/250‑2 (MQ=255)                                                                                                     gTAAAGTCTTCGTATACAAAAACACATTCAAACAATTTTTTTAACAACATCCATCATGAATTTAAGAAAAAAGATTGGATTTCGAATTATGATAGCCTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTTAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGTGCCGAATATACAATGACAtt                                                                                                           >  1:227282/1‑251 (MQ=255)                                                                                                      aaaaGTCTTCGTATACAAAAACACCTTCAAACAATTTTTTTAACAACATACATCATGCATTTAAGAAAAAAGATTGGATTTCGAATTATGATAGCCTGTTAACTTTGAGGGAATTCTTTCGTTGCGCAACTTAGATTGCTAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATACCGTTGCCGATTATACAATGACATTg                                                                                                          >  2:17033/2‑251 (MQ=255)                                                                                                                 tataCAAAAACACATTCAAACAATTTTTTTAACAACATCCATCATGCATTTAAGAAAAAAGATTGGATTTCGAATTATGATAGCCTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTTAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGTGCCGAATATACAATGACATTGCGAGGTAGTgg                                                                                               >  2:57776/1‑251 (MQ=255)                                                                                                                       aaaaCACATTCCAACGATTTTTTTAAAAACATCCATCATGCATTTAAGAAACAAGATTGGATTTCGTATTATGATAGCCTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAAATTAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGAGCCGAATATACAATGACCTGGCGAggaaggggttttg                                                                                          >  2:300214/1‑239 (MQ=255)                                                                                                                        aaaCACAGTTAAACAATTTTTTTAACAACATCCATCATGCATTTAAGAAAAAAGATTGGATTTCGAATTAGGATAGTCTGTTACCTTTTAGGGAGTGCTTTCGTTGCGCAAATTAGATTGATAAAAGTGGTTATCAGGTGCTTTCACCAAAAAATGAATCTGTTCACGCCATTGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGTGCCGAATATCCATTGACATTGCGAGGTTGTGGATCTGaa                                                                                        <  2:366762/251‑1 (MQ=255)                                                                                                                            acaTTCAAACAATTTTTTTAACAACATCCATCATGCATTTAAGAAAAAAGATTGGATTTCGAATTATGATAGCCTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTTAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGTGCCGAATATACAATGACATTGCGAGGTAGTGGATTTGAATACg                                                                                    <  2:112475/251‑1 (MQ=255)                                                                                                                                            tttAACAACATCCATCATGCATTTAAGAAAAAAGATTGGATTTCGAATTATGATAGCCTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTTAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGTGCCGAATATACAATGACATTGCGAGGTAGTGGATTTGAATACGAAGAAATACCCATAAc                                                                    >  1:439101/1‑251 (MQ=255)                                                                                                                                                 aaacaacCATGATGCATTTAAGAAAAAAGATTGGATTTCGAATTATGTTAACCTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAAATTAGCTTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGACATGGATAAGTTTCTTATCAGTTTCAGACTTAAAAATAACGGTGCCGAATATACAATGACATTGCGAGGTAGTGGATTTGAATACAAAGAAATACCCATAACAATa                                                                >  2:70333/2‑250 (MQ=255)                                                                                                                                                            atGCATTTAAGAAAAAAGATTGGATTTCGAATTATGATAGCCTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTTAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGTGCCGAATATACAATGACATTGCGAGGTAGTGGATTTGAATACGAAGAAATACCCATAACAATAAATGAATATAAc                                                    <  1:57776/251‑1 (MQ=255)                                                                                                                                                                aTTTAAGAAAAAAGATTGGATTTCGAATTATGATAGCCTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTTAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGTGCCGAATATACAATGACATTGCGAGGTAGTGGATTTGAATACGAAGAAATACCCATAACAATAAATGAATATAACtcat                                                >  1:41901/1‑251 (MQ=255)                                                                                                                                                                       aaaaaaGATTGGATTTCGAATTATGATAGCCTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTTAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGTGCCGAATATACAATGACATTGCGAGGTAGTGGATTTGAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATgg                                          >  1:310753/1‑250 (MQ=255)                                                                                                                                                                                     ttCGAATTATGATCGCCTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTTAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGTGCCGAATATACAATGACATTGCGAGGTAGTGAAtt                                                                                            >  1:432996/1‑186 (MQ=255)                                                                                                                                                                                     ttCGAATTATGATAGCCGGTTAACTTTGAGGGAGTTCTTTCGTTGAGCAACTTAGATTTATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGTGCCGAATATACAATGACATTGCGAGGTAGTGGAtt                                                                                            <  2:432996/186‑1 (MQ=255)                                                                                                                                                                                       cGAATTATGATAGCCTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTTAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGTGCCGAATATACAATGACATTGCGAGGTAGTGGATTTGAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGa                          >  1:141501/1‑250 (MQ=255)                                                                                                                                                                                                               tGAGGGAGTGCTTTCGTTGCGCAACTTAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGTGCCGAATATACAATGACATTGCGAGGTAGTGGATTTGAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACg  <  1:278315/250‑1 (MQ=255)                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                GAGATATTTAATGGCGACATAAAGTAACCAAATAAAATAAGGTTGTCATATGTTACCCAGGATCAGACACAATAATTTTATTGGTGCGGTGGAGTTATTTGTAAAGTCTTCGTATACAAAAACACATTCAAACAATTTTTTTAACAACATCCATCATGCATTTAAGAAAAAAGATTGGATTTCGAATTATGATAGCCTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTCAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGTGCCGAATATACAATGACATTGCGAGGTAGTGGATTTGAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACG  >  NC_000913/4437658‑4438113

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑