Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,344,501	G→A	G378D (GGC→GAC)	melB →	melibiose:H(+)/Na(+)/Li(+) symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,344,501	0	G	A	100.0%	143.7 / NA	47	G378D (GGC→GAC)	melB	melibiose:H(+)/Na(+)/Li(+) symporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (24/23);  total (24/23)

ATGGGCCGGAGCATCTATTCTTCCGGTGTTAAGCTGTGGTGTTCTCCTGTTAATGGCATTAATGAGCTATCACAACGTCGTCCTCATTGTGATTGCGGGTATTTTGCTGAATGTGGGAACGGCGCTTTTCTGGGTATTACAGGTCATCATGGTGGCAGATATCGTTGATTACGGTGAATATAAACTGCACGTACGCTGTGAAAGTATCGCTTACTCCGTGCAGACTATGGTGGTGAAGGGCGGTTCAGCCTTTGCGGCTTTTTTCATTGCGGTTGTGTTAGGGATGATTGGCTATGTACCGAATGTTGAACAGTCTACGCAAGCCCTATTAGGTATGCAGTTTATTATGATTGCTCTACCAACTCTGTTTTTCATGGTAACGCTGATTCTCTACTTCCGTTTCTATCGCCTCAATGGTGACACGCTGCGCAGGATCCAGATCCATCTGCTGGATAAATATCGCAAAGTACCGCCCGAGCCTG  >  NC_000913/4344262‑4344743                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                   aTGGGCCGGAGCATCTAATCTTCCGGTGTTAAGCTGTGGTGTTCTTCTGTTAATGGCATTAATGAGCTATCACAACGTCGTCCTCATGGTGATTGCGGGTATTTTGCTGAATGTGGGAACGGCGCTTTTCTGGGTATTACAGGTCATCATGGTGGCAGATATCGTTGATTACGGTGAATATAAACTGCACGTACGCTGTGTAAGTATCGCTTACTCCGTGCAGACTATTGTGGTGAAGGACGGTTCAGCCt                                                                                                                                                                                                                                         <  2:87712/251‑1 (MQ=255)      ccGGAGCATCTATTCTTCCGGTGTTAAGCTGTGGTGTTCTCCTGTTAATGGCATTAATGAGCTATCACAACGTCGTCCTCATTGTGATTGCGGGTATTTTGCTGAATGTGGGAACGGCGCTTTTCTGGGTATTACAGGTCATCATGGTGGCAGATATCGTTGATTACGGTGAATATAAACTGCACGTACGCTGTGAAAGTATCGCTTACTCCGTGCAGACTATGGTGGTGAAGGACGGTTCAGCCTTTGCg                                                                                                                                                                                                                                    >  1:167346/1‑251 (MQ=255)        ggAGCATCTATTCTTCCGGTGTTAAGCTGTGGTGTTCTCCTGTTAATGGCATTAATGAGCTATCACAACGTCGTCCTCATTGTGATTGCGGGTATTTTGCTGAATGTGGGAACGGCGCTGTTCTGGGTATTACAGTTCATCATGGTGGCAGATATCGTTGATTACGGTGAATATAAACTGCACGTACGCTGTGAAAGTATCGCTTACTCCGTGCAGACTATGGTGGTGAAGGACGGTTCAGCCTTTGCGGc                                                                                                                                                                                                                                  <  1:278211/251‑1 (MQ=255)         gAGCATCTATTCTTCCGGTGTTAAGCTGTGGTGTTCTCCTGTTAATGGCATTAATGAGCTATCACAACGTCGTCCTCATTGTGATTGCGGGTATTTTGCTGAATGTGGGAACGGCGCTTTTCTGGGTATTACAGGTCATCATGGTGGCAGATATCGTTGATTACGGTGAATATAAACTGCACGTACGCTGTGAAAGTATCGCTTACTCCGTGCAGACTATGGTGGTGAAGGACGGTTCAGCCTTTGCGGCt                                                                                                                                                                                                                                 <  1:75511/251‑1 (MQ=255)                       ccGGTGTTAAGCTGTGGTGTTCTCCTGTTAATGGCATTAATGAGCTATCACAACGTCGTCCTCATTGTGATTGCGGGTATTTTGCTGAATGTGGGAACGGCGCTTTTCTGGGTATTACAGGTCATCATGGTGGCAGATATCGTTGATTACGGTGAATATAAACTGCACGTACGCTGTGAAAGTATCGCTTACTCCGTGCAGACTATGGTGGTGAAGGACGGTTCAGCCTTTGCGGCTTTTTTCATTGCGGt                                                                                                                                                                                                                   >  1:42690/1‑251 (MQ=255)                                        tgtTCTCCTGTTAATGGCATTAATGAGCTATCACAACGTCGTCCTCATTGTGATTGCGGGTATTTTGCTGAATGTGGGAACGGCGCTTTTCTGGGTATTACAGGTCATCATGGTGGCAGATATCGTTGATTACGGTGAATATAAACTGCACGTACGCTGTGAAAGTATCGCTTACTCCGTGCAGACTATGGTGGTGAAGGACGGTTCAGCCTTTGCGGCTTTTTTCATTGCGGTTGTGTTAGGGATGATTg                                                                                                                                                                                                  >  1:11868/1‑251 (MQ=255)                                         gtTCTCCTGTTAATGGCATTAATGAGCTATCACAACGTCGTCCTCATTGTGATTGCGGGTATTTTGCTGAATGTGGGAACGGCGCTTTTCTGGGTATTACAGGTCATCATGGTGGCAGATATCGTTGATTACGGTGAATATAAACTGCACGTACGCTGTGAAAGTATCGCTTACTCCGTGCAGACTATGGTGGTGAAGGACGGTTCAGCCTTTGCGGCTTTTTTCATTGCGGTTGTGTTAGGGATGATTgg                                                                                                                                                                                                 <  2:167346/251‑1 (MQ=255)                                         gtTCTCCTGTTAATGGCATTAATGAGCTATCACAACGTCGTCCTCATTGTGATTGCGGGTATTTTGCTGAATGTGGGAACGGCGCTTTTCTGGGTATTACAGGTCATCATGGTGGCAGATATCGTTGATTACGGTGAATATAAACTGCACGTACGCTGTGAAAGTATCGCTTACTCCGTGCAGACTATGGTGGTGAAGGACGGTTCAGCCTTTGCGGCTTTTTTCATTGCGGTTGAGTTAGGGGTGATTgg                                                                                                                                                                                                 >  1:469426/1‑251 (MQ=255)                                              ccTGTTAATGGCATTAATGAGCTATCACAACGTCGTCCTCATTGTGATTGCGGGTATTTTGCTGAATGTGGGAACGGCGCTTTTCTGGGTATTACAGGTCATCATGGTGGCAGATATCGTTGATTACGGTGAATATAAACTGCACGTACGCTGTGAAAGTATCGCTTACTCCGTGCAGACTATGGTGGTGAAGGACGGTTCAGCCTTTGCGGCTTTTTTCATTGCGGTTGTGTTAGGGATGATTGGCTa                                                                                                                                                                                              <  1:315520/249‑1 (MQ=255)                                                     acggCATTAATAAGCTATCCCAACGTCGTCCTCATTGTGATTGCGGGTATTTTGCTGAATGTGGGAACGGCGCTTTTCTGGGTATTACAGGTAATCATGGTGGCAGAAATCGTTGATTAAGGTGAATATAAACTGCACGTACGCTGTGAAAGTATCGCTTACTCCGTGCAGACTATGGTGGTGAAGGACTGGTCAGACTTTGAGGCTTTTTTCATTGCGGGTGTGTTAgtgtggagtggctttgtaccagt                                                                                                                                                                                     >  2:105802/3‑229 (MQ=255)                                                             aaTGAGCTATCACAACGTCGTCCTCATTGTGATTGCGGGTATTTTGCTGAATGTGGGAACGGCGCTTTTCTGGGTATTACAGGTCATCATGGTGGCAGATATCGTTGATTACGGTGAATATAAACTGCACGTACGCTGTGAAAGTATCGCTTACTCCGTGCAGACTATGGTGGTGAAGGACGGTTCAGCCTTTGCGGATTTTTTCATTGCGGTTGTGTTAGGGATGATTGGCTATg                                                                                                                                                                                            >  1:292614/1‑236 (MQ=255)                                                             aaTGAGCTATCACAACGTCGTCCTCATTGTGATTGCGGGTATTTTGCGGAATGTGGGAACGGCGCTTTTCTGGGTATTTCAGGTCATCATGGTGGCAGATATCGTTGATTACGGTGAATATAAACTGCACGTACGCTGTGAAAGTATCGCTTACTCCGTGCAGACTATGGTGGTGAAGGACGGTTCAGCCTTTGCGGCTTTTTTCATTGCGGTTGTTTTATGGATGATTGGCTATg                                                                                                                                                                                            <  2:292614/236‑1 (MQ=255)                                                                gAGCTATCACAACGTCGTCCTCATTGTGATTGCGGGTATTTTGCTGAATGTGGGAACGGCGCTTTTCTGGGTATTACAGGTCATCATGGTGGCAGATATCGTTGATTACGGTGAATATAAACTGCACGTACGCTGTGAAAGTATCGCTTACTCCGTGCAGACTATGGTGGTGAAGGACGGTTCAGCCTTTGCGGCTTTTTTCATTGCGGTTGTGTTAGGGATGATTGGCTATGTACCGAATGTTGAACAg                                                                                                                                                                           <  1:465057/250‑1 (MQ=255)                                                                 aGCTATCTCAACGTCGTCCTCATTGTGATTGCGGGTATTTTGCTGAATGTGGGAACGGCGCTTTTCTGGGTATTACAGGTCATCATGGTGGCAGATATCGTTGATTACGGTGAATATAAACTGCACGTACGCTGTGAAAGTATCGCTTACTCCGTGCAGACTATGGTGGTGAAGGACGGTTCAGCCTTTGCGGCTTTTTTCATTGCGGTTGTGTTAGGGATGATTGGCTATGTACCGAATGTTGAACAGTc                                                                                                                                                                         <  1:375741/251‑1 (MQ=255)                                                                   cTATCACAACGTCGTCCTCATTGTGATTGCGGGTATTTTGCTGAATGTGGGTACGGCGCTTTTCTGGGTATTACAGGTCATCATGGTGGCAGATATCGTTGATTACGGTGAATATAAACTGCACGTACGCTGTGAAAGTATCGCTTACTCCGTGCAGACTATGGTGGTGAAGGACGGTTCAGCCTTTGCGGCTTTTTTCATTGCGGTTGTGTTAGGGATGCTTGGCTATGTACCGAATGTTGAACAGTCtc                                                                                                                                                                       >  1:473617/1‑250 (MQ=255)                                                                          aaCGTCGTCCTCATTGTGATTGCGGGTATTTTGCTGAATGTGGGAACGGCGCTTTTCTGGGTATTACAGGTCATCATGGTGGCAGATATCGTTGATTACGGTGAATATAAACTGCACGTACGCTGTGAAAGTATCGCTTACTCCGTGCAGACTATGGTGGTGAAGGACGGTTCAGCCTTTGCGGCTTTTTTCATTGCGGTTGTGTTAGGGATGATTGGCTATGTACCGAATGTTGAACAGTCAACGCAAGc                                                                                                                                                                >  1:344753/1‑251 (MQ=255)                                                                                     cATTGTGATTGCGGGTATTTTGCTGAATGTGGGAACGGCGCTTTTCTGGGTATTACAGGTCATCATGGTGGCAGATATCGTTGATTACGGTGAATATAAACTGCACGTACGCTGTGAAAGTATCGCTTACTCCGTGCAGACTATGGTGGCGAAGGACGGTTCAGCCTTTGCGGCTTTTTTCATTGCGGTTGTGTTAGGGATGATTGGCTATGTACCGAATGTTGAACAGTCTACGCAAGCCCTATTAGGTa                                                                                                                                                     <  2:151374/251‑1 (MQ=255)                                                                                          tgATTGCGGGTATTTTGCTGAATGTGGGAACGGCGCTTTTCTGGGTATTACAGGTCATCATGGTGGCAGATATCGTTGATTACGGTGAATATAAACTGCACGTACGCTGTGAAAGCATCGCTTACTCCGTGCAGACTATGGTGGTGAAGGACGGTTCAGCCTTTGCGGCTTTTTTCATTGCGGTTGTGTTAGGGATGGTTGGCTATGTCCCGTATGTTGCACAGTCTACGCAAGACATATTTGGTATga                                                                                                                                                  >  2:100374/1‑248 (MQ=255)                                                                                                cGGGGATTTTGCTGAATGTGGGAACGGCTCTTTTCTGGGTATTACTGGTCATCATGGTGGCAGATATCGTTGATTACGGTGAATATAAACTGCACGTACGCTGTGAAAGTATCGCTTACTCCGTGCAGACTATGGTGGTGAAGGACGGTTCAGCCTTTGCGGCTTTTTTCATTGCGGGTGTGTTAGGGATGATTGGCTATGTACCGAATGTTGAACAGTCTACGCAAGCCCTATTATGTATGCAGTttatt                                                                                                                                          <  2:458527/251‑1 (MQ=255)                                                                                                  ggTATTTTGCTGAATGTGGGAACGGCGCTTTTCTGGGTATTACAGGTCATCATGGTGGCAGATATCGTTGATTACGGTGAATATAAACTGCACGTACGCTGTGAAAGTATCGCTTACTCCGTGCAGACTATGGTGGTGAAGGACGGTTCAGCCttt                                                                                                                                                                                                                                       >  2:144862/1‑156 (MQ=255)                                                                                                  ggTATTTTGCTGAATGTGGGAACGGCGCTTTTCTGGGTATTACAGGTCATCATGGTGGCAGATATCGTTGATTACGGTGAATATAAACTGCACGTACGCTGTGAAAGTATCGCTTACTCCGTGCAGACTATGGTGGTGAAGGACGGTTCAGCCttt                                                                                                                                                                                                                                       <  1:144862/156‑1 (MQ=255)                                                                                                        ttGCTTAAGGTGGGAACGGCGTTTTTCTGGGTATTACAGGTCATCATGGTGGCAGATATCGTTGATTTCGGTGAATATAAACTGCACGTACGCTGTGAAAGTATCGCTTACTCCGTGCAGACTATGGTGGTGAAGGACGGTTCAGCCTTTGCGGCTTTTTTCATTGCGGTTGTGTTAGGGATGATTGGCTATGTACCGAATGTTGAACAGTCTACGCAAGCCCTATTAGGTATGCAGTTTATTATGGTTg                                                                                                                                   <  2:443549/250‑1 (MQ=255)                                                                                                            tGAATGTGGGAACGGCGCTTTTCTGGGTATTACAGGTCATCATGGTGGCAGATATCGTTGATTACGGTGAATATAAACTGCACGTACGCTGTGAAAGTATCGCTTACTCCGTGCAGACTATGGTGGTGAAGGACGGTTCAGCCTTTGCGGCTTTTTTCATTGCGGTTGTGTTAGGGATGATTGGCTATGTACCGAATGTTGAACAGTCTACGCAAGCCCTATTAGGTATGCAGTTTATTATGATTGctct                                                                                                                               >  1:346290/1‑250 (MQ=255)                                                                                                                   gggAACGGCGCTTTTCTGGGTATTACAGGTCATCATGGTGGCAGATATCGTTGATTACGGTGAATATAAACTGCACGTACGCTGTGAAAGTATCGCTTACTCCGTGCAGACTATGGTGGTGAAGGACGGTTCAGCCttt                                                                                                                                                                                                                                       >  1:251595/1‑139 (MQ=255)                                                                                                                   gggAACGGCGCTTTTCTGGGTATTACAGGTCATCATGGTGGCAGATATCGTTGATTACGGTGAATATAAACTGCACGTACGCTGTGAAAGTATCGCTTACTCCGTGCAGACTATGGTGGTGAAGGACGGTTCAGCCttt                                                                                                                                                                                                                                       <  2:251595/139‑1 (MQ=255)                                                                                                                               tttCTGGGTATTACAGGTCATCATGGTGGCAGATATCGTTGATTACGGTGAATATAAACTGCACGTACGCTGTGAAAGTATCGCTTACTCCGTGCAGACTATGGTGGTGAAGGACGGTTCAGCCTTTGCGGCTTTTTTCATTGCGGTTGTGTTAGGGATGATTGGCTATGTACCGAATGTTGAACAGTCTACGCAAGCCCTATTAGGTATGCAGTTTATTATGATTGCTCTACCAACTCTGTTTTTCATg                                                                                                            <  2:6521/250‑1 (MQ=255)                                                                                                                                           acagaacatcatGGGGGCAGATATCGTTGATTACGGTGAATATAAACTGCACGTACGCTGTGAAAGTATCGCTTACTCGGTGCAGCCTATGGTGGTAAAGGACGGTTCAGCCTTTGCGGCTTTTTTCCTTGCGGTTGTGTTAGGGCTGATTGGCTATGTACCGAATTTTGACCAGTCTACGCAAGCCCCATTCGGTATGCAGTTTATTCTGATTGCTCTACCAACTCTGTTTTTCATGTTAACGTTGAttt                                                                                               >  2:278696/7‑250 (MQ=255)                                                                                                                                             aGGTCATCATGGTGGCAGATATCGTTGATTACGGTGAATATAAACTGCACGTACGCTGTGAAAGTATCGCTTACTCCGTGCAGACTATGGTGGTGAAGGACGGTTCAGCCTTTGCGGCTTTTTTCATTGCGGTTGTGTTAGGGATGATTGGCTATGTACCGAATGTTGAACAGTCTACGCAAGCCCTATTAGGTATGCAGTTTATTATGATTGCTCTACCAACTCTGTTTTTCATGGTAACGCTGATtctc                                                                                             <  1:153529/251‑1 (MQ=255)                                                                                                                                               gTCATCATGGTGGCAGATATCGTTGATTACGGTGAATATAAACTGCACGTACGCTGTGAAAGTATCGCTTACTCCGTGCAGACTATGGTGGTGAAGGACGGTTCAGCCTTTGCGGCTTTTTTCATTGCGGTTGTGTTAGGGATGATTGGCTATGTACCGAATGTTGAACAGTCTACGCAAGCCCTATTAGGTATGCAGTTTATTATGATTGCTCTACCAACTCTGTTTTTCATGGTAACGCTGATTCTCTa                                                                                           <  2:11868/251‑1 (MQ=255)                                                                                                                                                    catGGTGGCAGATATCGTTGATTACGGTGAATATAAACTGCACGTACGCTGTGAAAGTATCGCTTACTCCGTGCAGACTATGGTGGTGAAGGACGGTTCAGCCTTTGCGGCTTTTTTCATTGCGGTTGTGTTAGGGATGATTGGCTa                                                                                                                                                                                              <  1:481792/147‑1 (MQ=255)                                                                                                                                                    catGGTGGCAGATATCGTTGATTACGGTGAATATAAACTGCACGTACGCTGTGAAAGTATCGCTTACTCCGTGCAGACTATGGTGGTGAAGGACGGTTCAGCCTTTGCGGCTTTTTTCATTGCGGTTGTGTTAGGGATGATTGGCTa                                                                                                                                                                                              >  2:481792/1‑147 (MQ=255)                                                                                                                                                    catGGGGGCAGATATCGTTGATTACGGTGAATATAAACTGCCCGTACGCTGTGAAAGTATCGCGTTCCCCGTGCAGACTATGGTGTGGAAGGACGGTTCAGCCTTTGCGGCGTTTTTCAGTGCGGTTGTGTTAGTGATGATTGGCTTTGTACCGAATGTTGAACAGTCTACGCAAGCCCTATTAGGTATGCAGGTTAGTATGATTGCGCTACCAACTCTGTGTTTCATGTTAACGCTGATTCTCTACTTcc                                                                                      <  2:473617/251‑1 (MQ=255)                                                                                                                                                              gATATCGTTGATTACGGTGAATATAAACTGCACGTACGCTGTGAAAGTATCGCTTACTCCGTGCAGACTATGGTGGTGAAGGACGGTTCAGCCTTTGCGGCTTTTTTCATTGCGGTTGTGTTAGGGATGATTGGCTATGTACCGAATGTTGAACAGTCTACGCAAGCCCTATTAGGTATGCAGTTTATTATGATTGCTCTACCAACTCTGTTTTTCATGGTAACGCTg                                                                                                   >  1:352337/1‑228 (MQ=255)                                                                                                                                                              gATATCGTTGATTACGGTGAATATAAACTGCACGTACGCTGTGAAAGTATCGCTTACTCCGTGCAGACTATGGTGGTGAAGGACGGTTCAGCCTTTGCGGCTTTTTTCATTGCGGTTGTGTTAGGGATGATTGGCTATGTACCGAATGTTGAACAGTCTACGCAAGCCCTATTAGGTATGCAGTTTATTATGATTGCTCTACCAACTCTGTTTTTCATGGTAACGCTg                                                                                                   <  2:352337/228‑1 (MQ=255)                                                                                                                                                                   cGTTGATTACGGTGAATATAAACTGCACGTACGCTGTGAAAGTATCGCTTACTCCGTGCAGACTATGGTGGAGAAGGACGGTTCAGCCTTTGCGGCTTTTTTCATTGCGGTTGTGTTAGGGATGATTGGCTATGTACCGAATGtt                                                                                                                                                                                 >  1:42879/1‑145 (MQ=255)                                                                                                                                                                   cGTTGATTACGGTGAATATAAACTGCACGTACGCTGTGAAAGTATCGCTTACGCCGTGCAGACTATGGTGGTGAAGGACGGTTCAGCCGTTGCGGCTTTTTTCATTGCGGTTGTGTTAGGGATGATTGGCTATGTACCGAATGtt                                                                                                                                                                                 <  2:42879/145‑1 (MQ=255)                                                                                                                                                                   cGTTGATTACGGTGAATATAAACTGCACGTACGATGTGAAAGTATCGCTTACCCCGTGCAGACTATGGCGGTGAAGGACGGCTCCGCCTTTGCGGCTTTTTTCATTGCGGTTGTGTTAGGGATGATTGGCTATGTACCGAATGTTGACAAGTCTACGCAAGCCCTATTAGGTCTGCAGTTTATTATGATTGCTCTACCAACTCTGTTTTTCATGGTAACGCTGTTTCTCTACTTCCGTTTCTATCGCCTc                                                                        >  2:237881/1‑250 (MQ=255)                                                                                                                                                                       gATTACGGAGAATATAAACTGCACGTACGCTGTGAAAGTATCGCTTACTCCGTGCAGACTATGGTGGTGAAGGACGGATCAGCCTTTGCGGCTTTTTTCATAGCGGTTGTGTTAGGGATGATTGGCTATGTACCGAATGTTGAACAGTCTACGCAAGCCCTATTAGGTATGCAGATTATTAAGATTGATCTACCAACTCTGTTTTTCATGGTAACGCTGATTCTCTAATTCCGTTTCTATAGACTCAATgg                                                                   >  1:462314/1‑251 (MQ=255)                                                                                                                                                                        aTTACGGTGAATATAAACTGCACGTACGCTGTGAAAGTATCGCTTACTCCGCGCAGACTATGGTGGTGAAGGACGGTTCAGCCTTTGCGGCTTTTTTCATTGCGGTTGTGTTAGGGATGATTGGCTATGTACCGAATGTTGAACAGTCTACGCAAGCCCTATTAGGTATGCAGTTTATTATGATTGCTCTACCAACTCTGTTTTTCATGGTAACGCTGATTCTCTACTTCCGTTTCTATCGCCTCAATGGt                                                                  >  1:379349/1‑251 (MQ=255)                                                                                                                                                                           cctgTGAATATATACTGCACGTACGCTGTGAAAGTATCGCTTACTCCGTGCAGACTATGGTGGTGAAGGACGGTTCAGCCTTTGCGGCTTTTTTCATTGCGGTTGTGTTAGGGATGATTGGCTATGTACCGAATGTTGAACAGTCTACGCAAGCCCTATTAGGTATGCAGTTTATTATGATTGCTCTACCAACTCTGTTTTTCATGGTAACGCTGATTCTCTACTTCCGTTTCTATCGCCTCAATGGTGa                                                                <  1:73145/247‑1 (MQ=255)                                                                                                                                                                            cGGTGAATATAAACTGCACGTACGCTGTGAAAGTATCGCTTACTCCGTGCAGACTATGGTGGTGAAGGACGGTTCAGCCTTTGCGGCTTTTTTCATTGCGGTTGTGTTAGGGATGATTGGCTATGTACCGAATGTTGAACAGTCTACGCAAGCCCTATTAGGTATGCAGTTTATTATGATTGCTCTACCAACTCTGTTTTTCATGGTAACGCTGATTCTCTACTTCCGTTTCTATCGCCTCAATGGTGac                                                               >  1:2656/1‑250 (MQ=255)                                                                                                                                                                                            cACGTACGATGTGAAAGTATCGCTTACTCCGTGCAGACTATGGTGGTGAAGTACGGTTCAGCCTTTGCGGCTTTTTTCATTGCGGTTGTGTTAGGGAGGATTGGCTATGTACCGAATGTTGCACAGTCTACGCAAGCCCTATTAGGTATGCAGTTTATTATGAATGCTCTACCAACTCTGTTTTTCATGGAAACGCTGATTCTCTACTTCCGTTTCTATCGCCTCAATTGTGACACGCTGCGCAGggtcc                                               >  2:21330/1‑250 (MQ=255)                                                                                                                                                                                               gTACGCTGTGAAAGTATCGCTTACTCCGTGCAGACTATTGTGGGGAAGGACGGTTCAGCCTTTGCGGCTTTTTTCATTGCGGTTGTGTTAGGGATGATTGGCTGTGTACCGAATGTTGAACAGTCTACGCAAGCCCTATTAGGTATGCAGTTTATTATGATTGCTCTACCAACTCTGTTTTTCATGGTAACGCTGACTCTCTACTTCCGGTTCGATCTCCGCAATTGTGACACGCTGCGCAGtatccagat                                           <  2:64271/251‑1 (MQ=255)                                                                                                                                                                                                             tATCGCTTACTCCGTGCAGACTATGGTGGTGAAGGACGGTTCAGCCTTTGCGGCTTTTTTCATTGCGGTTGTGTTAGGGATGATTGGCTATGTACCGAATGTTGAACAGTCTACGCAAGCCCTATTAGGTATGCAGTTTATTATGATTGCTCTACCAACTCTGTTTTTCATGGTAACGCTGATTCTCTACTTCCGTTTCTATCGCCTCAATGGTGACACGCTGCGCAGGATCCAGATCCATCTGCTGGAt                              >  1:425414/1‑250 (MQ=255)                                                                                                                                                                                                                            gCAGACTATGGTGGTGAAGGACGGTTCAGCCTTTGCGGCTTTTTTCATTGCGGTTGTGTTAGGGATGATTGGCTATGTACCGAATGTTGAACAGTCTACGCAAGCCCTATTAGGTATGCAGTTTATTATGATTGCTCTACCAACTCTGTTTTTCATGGTAACGCTGATTCTCTACTTCCGTTTCTATCGCCTCAATGGTGACACGCTGCGCAGGATCCAGATCCATCTGCTGGATAAATATCGCAAAGTAc              >  1:350013/1‑251 (MQ=255)                                                                                                                                                                                                                              aGACTATGGTGGTGAAGGACGGTTCAGCCTTTGCGGCTTTTTTCATTGCGGTTGTGTTAGGGATGATTGGCTATGTACCGAATGTTGAACAGTCTACGCAAGCCCTATTAGGTATGCAGTTTATTATGATTGCTCTACCAACTCTGTTTTTCATGGTAACGCTGATTCTCTACTTCCGTTTCTATCGCCTCAATGGTGACACGCTGCGCAGGATCCAGATCCATCTGCTGGATAAATATCGCAAAGTACCg            >  1:325014/1‑251 (MQ=255)                                                                                                                                                                                                                                        ggtgAAGGACGGTTCAGCCTTTGCGGCTTTGTTCATTGCGGGTGTGTTAGGGATGATTGGCTATGTACCGAATGTTGAACAGTCTACGCAAGCCCTATTAGGTATGCAGTTTATTATGATTGCTCTACCAACTCTGTTTTTCATGGTAACGCTGATTCTCTACTTCCGTTTCTATCGCCTCAATGGTGACACGCTGCGCAGGATCCAGATCCATCTGCTGGATAAATATCGCAAAGTACCGCCCGAGCCTg  <  2:101809/251‑1 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                   ATGGGCCGGAGCATCTATTCTTCCGGTGTTAAGCTGTGGTGTTCTCCTGTTAATGGCATTAATGAGCTATCACAACGTCGTCCTCATTGTGATTGCGGGTATTTTGCTGAATGTGGGAACGGCGCTTTTCTGGGTATTACAGGTCATCATGGTGGCAGATATCGTTGATTACGGTGAATATAAACTGCACGTACGCTGTGAAAGTATCGCTTACTCCGTGCAGACTATGGTGGTGAAGGGCGGTTCAGCCTTTGCGGCTTTTTTCATTGCGGTTGTGTTAGGGATGATTGGCTATGTACCGAATGTTGAACAGTCTACGCAAGCCCTATTAGGTATGCAGTTTATTATGATTGCTCTACCAACTCTGTTTTTCATGGTAACGCTGATTCTCTACTTCCGTTTCTATCGCCTCAATGGTGACACGCTGCGCAGGATCCAGATCCATCTGCTGGATAAATATCGCAAAGTACCGCCCGAGCCTG  >  NC_000913/4344262‑4344743

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑