Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 867 C→T S177S (AGC→AGT thrA → fused aspartate kinase/homoserine dehydrogenase 1

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009138670CT96.3% 77.3 / ‑4.0 28S177S (AGC→AGTthrAfused aspartate kinase/homoserine dehydrogenase 1
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base T (26/0);  total (28/0)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.39e-01

ATCAGGAATTTGCCCAAATAAAACATGTCCTGCATGGCATTAGTTTGTTGGGGCAGTGCCCGGATAGCATCAACGCTGCGCTGATTTGCCGTGGCGAGAAAATGTCGATCGCCATTATGGCCGGCGTATTAGAAGCGCGCGGTCACAACGTTACTGTTATCGATCCGGTCGAAAAACTGCTGGCAGTGGGGCATTACCTCGAATCTACCGTCGATATTGCTGAGTCCACCCGCCGTATTGCGGCAAGCCGCATTCCGGCTGATCACATGGTGCTGATGGCAGGTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTGCTTGGACGCAACGGTTCCGACTACTCTGCTGCGGTGCTGGCTGCCTGTTTACGCGCCGATTGTTGCGAGATTTGGACGGACGTTGACGGGGTCTATACCTGCGACCCGCGTCAGGTGCCCGATGCGAGGTTGTTGAAGTCGATGTCCTACCAGGAAGCGATGGAGCTT  >  NC_000913/620‑1107
                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                 
aTCAGGAATTTGCCCAAATAAAACATGTCCTGCATGGCATTAGTTTGTTGGGGCAGTGCCCGGATAGCATCAACGCTGCGCTGATTTGCCGTGGCGAGAAAATGTCGATCGCCATTATGGCCGGCGTATTAGAAGCGCGCGGTCACAACGTTACCGTTATCGATCCGGTCGAAAAACTGCTGGCAGTGGGGCATTACCTCGAATCTACCGTCGATATTGCTGAGTCCACCCGCCGACTTGCGGCAAGTCGc                                                                                                                                                                                                                                                >  2:136580/1‑251 (MQ=255)
             ccAAATAAAACATGTCCTGCATGGCATTAGTTTGTTGGGGCAGTGCCCGGATAGCATCAACGCTGCGCTGATTTGCCGTGGCGAGAAAATGTCGATCGCCATTATGGCCGGCGTATTAGAAGCGCGCGGTCACAACGTTACCGTTATCGATCCGGTCGAAAAACTGCTGGCAGTGGGGCATTACCTCGAATCTACCGTCGATATTGCTGAGTCCACCCGCCGTATTGCGGCAAGTCGCATTCCGGCTGATc                                                                                                                                                                                                                                   >  1:253073/1‑251 (MQ=255)
                   aaaaCATGTCCTGCACGGCCTTAGCTTGTTGGGGCAGTGCCCGAATAGCATCAACGCTGCGATGATTTGACGTGGCGAGAAAATGTCGATCGACATTATGGCCGGCGTATTAGCAGCGCGCGGTCACAACGCTCCCGTTATCGATTCGGTCGAAAAACTGCTGGCAGTGGGGCATTACCTCGAATCTACCGTCGCTATACCTGAGTCCACCCGCCGTATTGCGTCAAGTCGCATTCCGGCTGATccgatgg                                                                                                                                                                                                                             >  2:233208/1‑245 (MQ=255)
                     aaCATGTCCTGCATGGCATTAGTTTGTTGGGGCAGTGCCCGGATAGCATCAACGCTGCGCTGATTTGCCGTGGCGCGAAAATGTCGATCGCCATTATGGCCGGCGTATTAGGAGCGCGCGGTCACAACGTTACCGTTATCGATCCGGTCGAAAAACTGCTGGCAGTGGGGCTTTACCTCGCATCTACCGCCGATTTTGCTGAGTCCACCCGCCGTATTGCGGCAAGTCGCATTCCGGCTGATCAAATgttg                                                                                                                                                                                                                           >  2:432545/1‑248 (MQ=255)
                                                                         cgctgcgctgATTTGCCGTGGCGAGAAAATGTCGGTCGCCATTATGGCCGGCGTATTAGAGGCGCGCGGTCACCACGTTACCGTTATCGATCCGGTCGAAAAACTGCTGGCAGTGGGGCATTACATCGCATCTAACGTCGATATTGCTGAGTCCACCCGCCGTATTGCGGCAAGTAGCCTTCCGGCTGATCACATGGTGCAGATGGCAGGTTTCAACGCCGGGAATGAAAAAgggggacaggggggagttt                                                                                                                                                                       >  2:279987/1‑234 (MQ=255)
                                                                                       gCCGTGGCGAGAAAATGTCGATCGCCATTATGTCCGGCGTATTAGAAGCGCGCGGTCACAACGTTACCGTTATCGATCCGGTCGAAAAACTGCTGGCCGTGGGGCATTACCTCGAATCTACCGTCGATATTGCTGAGTCCACCCGCCGTATTGCGGCAAGTCGCATTCCGGCTGATCACATGGGGCTGATGGCAGGTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGGACTTGGACGCAACGGTTcc                                                                                                                                                         >  1:35869/1‑251 (MQ=255)
                                                                                           tcgCGAGAAAATGTCGATCGCCATTATGGCCGGCGTATTAGAAGCGCGCGGTCACAACGTTACCGTTATCGATCCGGTCGAAAAACTGCTGGCAGTGGGGCATTACCTCGAATCTACCGTCGATATTGCTGAGTCCACCCGCCGTATTGCGGCAAGTCGCATTCCGGCTGATCACATGGTGCTGCTGGCAGGTTTcccc                                                                                                                                                                                                         >  2:102151/3‑196 (MQ=255)
                                                                                                 gaAAATGTCGCCCGCCATTCTAGCCGGCGTCTTAGAAGGGCGCGGTCACAAAGTTACCGTTATCGATCCGGTCGAAAAACTGCTGGCCGTGGGGCATTACCTCGAATCTACCGTCGATATTGCTGAGTCCCCCCGCCGTATTGCGGCAAGACGCCTTCCGGc                                                                                                                                                                                                                                        >  2:318504/1‑162 (MQ=255)
                                                                                                  aaaaTGTCGATCGCCATTATGGCCGGCGTATTAGAAGCGCGCGGTCACAACGTTACCGTTATCGATCCGGTCGAAAAACTGCTGGCAGTGGGGCATTACCTCGAATCTACCGTCGATATTGCTGAGTCCACCCGCCGTATTGCGGCAAGTCGCATTCCGGCTGATCACATGGTGCTGATGGCAGGTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTACTTGGACGCAACGGTTCCGACTActccgc                                                                                                                                              >  1:189956/1‑248 (MQ=255)
                                                                                                             cGCCATTATGGCCGGCGGATTAGAAGCGCGCGGTCACAACGTTACCGTTATCGATCCGGTCGAAAAACTGCTGGCAGTGGGGCATTACCTCGAATCTACCGTCGATATTGCTGAGTCCACCCGCCGTATTGCGGCAAGTCGCATTCCGGCTGATCACATGGTGCTGATGGCAGGTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTACTTGGACGCAACGGTTCCGCCTActccccggcggtgccgg                                                                                                                                   >  2:63015/1‑237 (MQ=255)
                                                                                                               ccATTAAGGCCGGCGTATTAGACGCGCGCGGTCACAACGTTACCGTTATCGATCCGGTCGAAAAACTGCTGGCAGTGGGGCATTACCTCGAATCTACCGTCGTTATTGCTGAGTCCACCCGCCGTATTGCGGCAAGTCGCATTCCGGCTGATCACATGGTGCTGATGGCAGGATTCACTGCCGGTAATGACAAAGGCGACCTGGTGTTACTTTGACGCAACGGTTCCGACTACTCCGCGGCGGTgccggct                                                                                                                                 >  1:302491/1‑251 (MQ=255)
                                                                                                                             gTATTAGAAGCGCGCGGTCACAACGTTACCGTTATCGATCCGGTCGAAAAACTGCTGGCAGTGGGGCATTACCTCGAATCTACCGTCGATATTGCTGAGTCCACCCGCCGTATTGCGGCAAGTCGCATTCCGGCTGATCACATGGTGCTGATGGCAGGTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTCCTTGGACGCAACGGTTCCGACTACTCCGCGGCGGTGCTGGATGCCTGTTTACgcgc                                                                                                                   >  1:21671/1‑251 (MQ=255)
                                                                                                                                    aaGCGCGAGGTCACAACGTTACCGTTATCGATCCGGTCGAAAAACTGCTGGCAGTGGGACATTACCTCGAATCTACCGTCGAAATTGCTGAGTCCCCCCGCCGTATTGCGCCAAGTCGCATTCCGGCTGCTCACATGGTGCTGATGGCAGGTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTACTTGGACGCAACGGTTCCGCCTACTACGCGGCGGAGCTGGCTGCCAGTTTATGCGACGActgt                                                                                                            >  1:313276/1‑251 (MQ=255)
                                                                                                                                            ggTCACAACGTTACCGTTATCGATCCGGTCGAAAAACTGCTGGCAGTGGGGCATTACCTCGAATCTACCGTCGATATTGCTGAGTCCACCCGCCGTATTGCGGCAAGTCGCATTCCGGCTGATCACATGGTGCTGATGGCAGGTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTACTTGGACGCAACGGTTCCGACTACTCCGCGGCGGTGGTGGGTGCCCTTTTTCGCGCCCGTTGTTGCgaggt                                                                                                    >  2:175749/1‑249 (MQ=255)
                                                                                                                                                acaACGTTACTGTTATCGATCCGGTCGAAAAACTGCTGGCAGTGGGGCATTACCTCGAATCTACCGTCGATATTGCTGAGTACACCCGCCGTATTGCGGCAAGCCGCATTCCGGCTGATCACATGGTGCTGATGGCAGGTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTGCTTGGACGCAACGGTTCCGACTAATCCGCTGCGGTGCTGGCTGCCTGTTTACGCGCCGATTGTTGctagcttttg                                                                                                >  2:142420/1‑242 (MQ=255)
                                                                                                                                                acaACGTTACCGTTATCGATCCGGTCGAAAAACTGCTGGCAGTGGGGCATTACCTCGAATCTACCGTCGATATTGCTGAGTCCACCCGCCGTATTGCGGCAAGTCGCATTCCGGCTGATCACATGGTGCTGATGGCAGGTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTACTTGGACGCAACGGTTCCGACTACTCCGCGGCGGTGCTGGCTGCCTGTTTACGCGCCGATTGTTGCGAGATTTgg                                                                                                >  1:318425/1‑251 (MQ=255)
                                                                                                                                                     gTTACCGTTATCGATCCGGTCGAAAAACTGCTGGCAGTGGGGCATTACCTCGAATCTACCGTCGATATTGCTGAGTCCACCCGCCGTATTGCGGCAAGTCGCATTCCGGCTGATCACATGGTGCTGATGGCAGGTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTACTTGGACGCAACGGTTCCGACTACTCCGCGGCGGTGCTGGCTGCCTGTTTACGCGCCGATTGTTGCGAGATTTggacggg                                                                                           >  1:183809/1‑250 (MQ=255)
                                                                                                                                                      ttACCGTTATCGATCCGGTCGAAAAACTGCTGGCAGTGGGGCATTACCTCGAATCTACCGTCGATATTGCTGAGTCCAACCGCCGTATTGCGGCAAGTCGCATTCCGGCTGATCACATGGTGCTGCTGGCAGGTTTCACCGCCGGTCATGAAAAAGGCGAACTGGTGGTACTTGGACGCAACGGTTCCGACTACTCAGCGGCGGGTCTGGCTGCCTGTTTTAGCGCCGGttgttggcggtattgtgaggac                                                                                          >  2:466144/1‑235 (MQ=255)
                                                                                                                                                                    ccGGTCGAAAAACTGCTGGCAGTGGGGCATTACCTCGAATCTACCGTCGATCTTGCTGAGTCCACCCGCCGTATTGCGGCAAGTCGCATTCCGGCTGATCACATGGTGCTGATGGCAGGTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTACTTGGACGCAACGGTTCCGACTACTCCGCGGCGGTGCTGGCTGCCTGTTTACGCTCCGATTGTTGCGAGATTTGGATGGACGTTGTCGGGGTCtt                                                                           >  1:46057/1‑250 (MQ=255)
                                                                                                                                                                     cGGTCGAAAAACTGCTGGCAGTGGGGCATTACCTCGAATCTACCGTCGATATTGCTGAGTCCACCCGCCGTATTGCGGCAAGTCGCATTCCGGCTGATCACATGGTGCTGATGGCAGGTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTACTTGGACGCAACGGTTCCGACTACTCCGCGGCGGTGCTGGCTGCCTTGTTACCCGCCGCCTTTTGCGTGATTTGGACGGGCGtttgcggagtgttt                                                                          >  2:184590/1‑239 (MQ=255)
                                                                                                                                                                         cGAAAAACTGCTGGCAGTGGGGCATTACCTCGAATCTACCGTCGATATTGCTGAGTCCACCCGCCGTATTGCGGCAAGTCGCATTCCGGCTGATCACATGGTGCTGATGGCAGGTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTACTTGGACGCAACGGTTCCGACTACTCCGCGGCGGGGGCGGCTGCCTGTTTACGCGCCGATTTTTGCGGGGTTGTGACGGGACGTTGAGGGGGtgtttacc                                                                       >  2:332005/1‑244 (MQ=255)
                                                                                                                                                                             aaaCTGCTGGCAGTGGGGCATTACCTCGAATCTACCGTCGATATTGCTGAGTCCACCCGCCGTATTGCGGCACGTCGCATTCCGGCTGATCCCATGGTGCTGATGGCAGGTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTACTTGGACGCAACGGTTCCGACTACTCCGCGGCGGTGCTGGCTGCCCGTTTCCCCGCCGGTTGTTGCGGGGTTTGGACGGACGTTGACGGGGGCTTTACCTGCgc                                                                  >  1:163257/1‑250 (MQ=255)
                                                                                                                                                                                  gctgGCAGTGGGGCATTACCTCGAATCTACCGTCGATATTGCTGAGTCCACCCGCCGTATTGCGGCAAGTCGCATTCCGGCTGATCACATGGTGCTGATGGCAGGTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTACTTGGACGCAACGGTTCCGACTACTCCGCGGCGGTGCGGGGTGCCTGTTTTCGCGCCGATTGGTGCGGGGATTGGGCGGACGTTGACGGGGGCTATACCTGCGGCCcgc                                                             >  2:126821/1‑251 (MQ=255)
                                                                                                                                                                                                                 gTCGATATTGCTGAGTCCACCCGCCGTATTGCGGCAAGTCGCATTCCGGCTGATCACATGGTGCTGATGGCAGGTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTACTTGGACGCAACGGTTCCGACTActccgcg                                                                                                                                             >  1:415229/1‑137 (MQ=255)
                                                                                                                                                                                                                 gTCGATATTGCTGAGTCCACCCGCCGTATTGCGGCAAGTCGCATTCCGGCTGATCACATGGTGCTGATGGCAGGTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTACTTGGACGCAACGGTTCCGACTACTCCGCGGCGGTGCTGGCTGCCTGTTTACGCGCCGATTGTTGCGAGATTTGGACGGACGTTGACGGGGTCTATACCTGCGACCCGCGTCAGGTGCCCGATGCGAGGTTGTTGAAGTc                              >  1:98955/1‑251 (MQ=255)
                                                                                                                                                                                                                                   aCCCGCCGTATTGCGGCAAGTCGCATTCCGGCTGATCACATGGTGCTGATGGCAGGTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTACTTGGACGCAACGGTTCCGACTACTCCGCGGCGGGGCTGGCTGCCTGTTTACGCGCCGATTGTTGCGAGATTTGGACGGACGGTGACGGGGGCTATACCTGGGACCCGCGTCAGGTGCCCGATGCGGGGTTGGTGAAGTCGATGGCCTACCAGGAAGc            >  1:127743/1‑251 (MQ=255)
                                                                                                                                                                                                                                         cgTATTGCGGCAAGTCGCATTCCGGCTGATCACATGGTGCTGATGGCAGGTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTACTTGGACGCAACGGTTCCGACTACTCCGCGGCGGTGCTGGCTGCCTGTTTACGCGCCGATTGTTGCGAGATTTGGACGGACGTTGACGGGGTCTATACCTGCGACCCGCGTCAGGTGCCCGATGCGAGGTTGTTGAAGTCGATGTCCTACCAGGAAGCGATggg      >  1:83974/1‑250 (MQ=255)
                                                                                                                                                                                                                                             ttGCGGCAAGTCGCATTCCGGCTGATCACATGGTGCTGATGGCAGGTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTACTTGGACGCAACGGTTCCGACTACTCCGCGGCGGTGCTGGCTGCCTGTTTACGCGCCGATTGTTGCGAGATTTGGACGGACGTTGACGGGGTCTATACCTGCGACCCGCGTCAGGGGCCCGATGCGAGGGTGTTGAAGTCGATGTCCTACCAGGAAGCGATGGGGCtt  >  1:304090/1‑251 (MQ=255)
                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                 
ATCAGGAATTTGCCCAAATAAAACATGTCCTGCATGGCATTAGTTTGTTGGGGCAGTGCCCGGATAGCATCAACGCTGCGCTGATTTGCCGTGGCGAGAAAATGTCGATCGCCATTATGGCCGGCGTATTAGAAGCGCGCGGTCACAACGTTACTGTTATCGATCCGGTCGAAAAACTGCTGGCAGTGGGGCATTACCTCGAATCTACCGTCGATATTGCTGAGTCCACCCGCCGTATTGCGGCAAGCCGCATTCCGGCTGATCACATGGTGCTGATGGCAGGTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTGCTTGGACGCAACGGTTCCGACTACTCTGCTGCGGTGCTGGCTGCCTGTTTACGCGCCGATTGTTGCGAGATTTGGACGGACGTTGACGGGGTCTATACCTGCGACCCGCGTCAGGTGCCCGATGCGAGGTTGTTGAAGTCGATGTCCTACCAGGAAGCGATGGAGCTT  >  NC_000913/620‑1107

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: