Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,288,385	G→A	V208I (GTT→ATT)	nrfA →	cytochrome c552 nitrite reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,288,385	0	G	A	100.0%	138.3 / NA	44	V208I (GTT→ATT)	nrfA	cytochrome c552 nitrite reductase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (22/22);  total (22/22)

TAAAAGCCCGGATGTGGCGCGTCTGATCCAGAAAGACGGCGAAGATGGCTACTTCCACGG‑TAAATGGGCGCGCGGCGGTCCGGAAATCGTCAACAACTTAGGTTGTGCCGATTGCCATAACACCGCCTCTCCAGAGTTCGCCAAAGGCAAACCGGAGTTAACCCTTTCCCGTCCGTATGCGGCTCGCGCGATGGAAGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGTGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTAAATTCCCGTGGGATGACGGCATGAAAGTCGAAAATATGGAGCAGTATTACGACAAAATTGCCTTCTCTGACTGGACTAACTCCCTGTCGAAAACGCCAATGCTGAAAGCGCAGCACCCGGAATATGAAACCTGGACAGCGGGCATTCACGGTAAAAACAACGTGACCTGTATCGACTGCCAT  >  NC_000913/4288138‑4288621                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                             tAAAAGCCCGGATGTGGCGCGTCTGATCCAGAAAGACGGCGAAGATGGCTACTTCCACGG‑TAAATGGGCGCGCGGCGGTCCGGAAATCGTCAACAACTTAGGTTGTGCCGATTGCCATAACACCGCCTCTCCAGAGTTCGCCAAAGGCAAACCGGAGTTAACCCTTTCCCGTCCGTATGCGGCTCGCGCGATGGAAGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGAttt                                                                                                                                                                                                                                           >  1:409457/1‑251 (MQ=255)  aaaaGCCCGGATGTGGCGCGTCTGATCCAGAAAGACGGCGAAGATGGCTACTTCCACGG‑TAAATGGGCGCGCGGCGGTCCGGAAATCGTCAACAACTTAGGTTGTGCCGATTGCCATAACACCGCCTCTCCAGAGTTCGCCAAAGGCAAACCGGAGTTAACCCTTTCCCGTCCGTATGCGGCTCGCGCGATGGAAGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGATTTg                                                                                                                                                                                                                                          >  1:128631/1‑251 (MQ=255)      gCCCGGATGTGGCGCGTCTGATCCAGAAAGACGGCGAAGATGGCTACTTCCACGG‑TAAATGGGCGCGCGGCGGTCCGGAAATCGTCAACAACTTAGGTTGTGCCGATTGCCATAACACCGCCTCTCCAGAGTTCGCCAAAGGCAAACCGGAGTTAACCCTTTCCCGTCCGTATGCGGCTCGCGCGATGGAAGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGATTTGCGGt                                                                                                                                                                                                                                      >  1:144846/1‑251 (MQ=255)        cacccgcgctcttcccagtcccaaaaaGACGGCGAAGAGGGCTACT‑CCACGGGAAAAGGGGCGCGCGGGGGCCCGGAAATCGAAAACACCTTGGGTGGTGCAGGTGGCCATACCACCGCCTCTCCAGAGTTCGCCAAAGGCAAACCGGAGTTAACCCTTTCCCGTCCGTATGCGGCTCGCGCGATGGAAGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGATTTGCGGTCa                                                                                                                                                                                                                                    <  2:106162/227‑1 (MQ=255)          ggATGTGGCGCGTCTGATCCAGAAAGACGGCGAAGATGGCTACTTCCACGG‑TAAATGGGCGCGCGGCGGTCCGGAAATCGTCAACAACTTAGGTTGTGCCGATTGCCATAACACCGCCTCTCCAGAGTTCGCCAAAGGCAAACCGGAGTTAACCCTTTCCCGGCCGTATGCGGCTCGCGCGATGGAAGCCATTGGTAAACCTTTTGCGAAAGCCGGACGTTTCGACCAGCAATCGATGATTTGCGGTCAgg                                                                                                                                                                                                                                  >  1:137745/1‑250 (MQ=255)             tggggCGCGTTGGATCCAGAAAGGCGGCGAATATGTCTACTCCCACGG‑TAAAGGGGCGCGCGGCTGGCCGGAAATCTTCAACAACTTAGTTTGTGCCGATTGCCATAACACCGCCTCTCAAGAGTTCGCCAAAGGCAAACCGGAGTTAACCCTTTCCCGTCCGTATGCGGCTCGCGCGATGGAAGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGATTTGCGGTCAGTGc                                                                                                                                                                                                                                <  1:18036/247‑1 (MQ=255)              gtgGCGCGTCTGATCCAGAAAGACGGCGAAGATGGCTACTTCCACGG‑TAAATGGGCGCGCGGCGGTCCGGAAATCGTCAACAACTTAGGTTGTGCCGATTGCCATAACACCGCCTCTCCAGAGTTCGCCAAAGGCAAACCGGAGTTAACCCTTTCCCGTCCGTATGCGGCTCGCGCGATGGAAGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGAAATCGATGATTTGCGGTCAGTGcc                                                                                                                                                                                                                               >  1:86899/1‑250 (MQ=255)                             cAGAAAGACGGCGAAGATGGCTACTTCCACGG‑TAAATGGGCGCGCGGCGGTCCGGAAATCGTCAACAACTTAGGTTGTGCCGATTGCCATAACACCGCCTCTCCAGAGTTCGCCAAAGGCAAACCGGAGTTAACCCTTTCCCGTCCGTATGCGGCTCGCGCGATGGAAGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGATTTGCGGTCa                                                                                                                                                                                                                                    >  1:106162/1‑230 (MQ=255)                                     cGGCGAAGATGGCTACTTCCACGG‑TAAATGGGCGCGCGGCGGTCCGGAAATCGTCAACAACTTAGGTTGTGCCGATTGCCATAACACCGCCTCTCCAGAGTTCGCCAAAGGCAAACCGGAGTTAACCCTTTCCCGTCCGTATGCGGCTCGCGCGATGGAAGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGATTTGCGGTCAGTGCCATGTGGAGTATTACTTCGACGGc                                                                                                                                                                                                        >  2:168696/1‑250 (MQ=255)                                                gCTACTTCCACGG‑TAAATGGGCGCGCGGCGGTCCGGAAATCGTCAACAACTTAGGTTGTGCCGATTGCCATAACACCGCCTCTCCAGAGTTCGCCAAAGGCAAACCGGAGTTAACCCTTTCCCGTCCGTATGCGGCTCGCGCGATGGAAGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGAAATCGATGATTTGCGGACAGTGCCATGTGGAGTAATACTTCGCCGGAAAAAACAAAGCg                                                                                                                                                                                            >  2:276043/1‑251 (MQ=255)                                                           gggaaagtGGGCGGGGGGCGGGCCGGAAACCGCAAACAACTAAGGTTGCCCCGATTCCAAAAACACCGCCTCTCCAGAGTTCGCCAAAGGCAAACCGGAGTTAACCCTTTCCCGTCCGTATGCGGCTCGCGCGATGGAAGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGATTTGCGGTCAGTGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTAAATTcc                                                                                                                                                                                  <  2:409457/244‑1 (MQ=255)                                                                              ggccgggAATTCGTAAACAACTTAGGTTGGGCCGTTTCCCATACCACCGCCTCTCCAGAGTTCGCCAAAGGCAAACCGGAGTTAACCCTTTCCCGTCCGTATGCGGCTCGCGCGATGGAAGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGATTTGCGGTCAGTGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTAAATTCCCGTGGGATGACGGCATGaa                                                                                                                                                               <  2:167691/246‑1 (MQ=255)                                                                                   ggAAATCGTCAACAACTTAGGTTGTGCCGATTGCCATAACACCGCCTCTCCAGAGTTCGCCAAAGGCAAACCGGAGTTAACCCTTTCCCGTCCGTATGCGGCTCGCGCGATGGAAGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGATTTGCGGTCAGTGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTAAATTCCCGTGGGATGACGGCATGAAAGTCg                                                                                                                                                          >  2:42163/1‑251 (MQ=255)                                                                                       atcgaaaaaaatttAGGTTGGGCCGATTGCAATAACCCCGCCTCTCCAGAGTTCGCCAAAGGCAAACCGGAGTTAACCCTTTCCCGTCCGTATGCGGCTCGCGCGATGGAAGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGATTTGCGGTCAGTGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTAAATTCCCGTGGGATGACGGCATGAAAGTCGaaaa                                                                                                                                                      <  1:425277/239‑1 (MQ=255)                                                                                       aTCGTCAACAACTTAGGTTGTGCCGATTGCCATAACACCGCCTCTACAGAGTTCGCCAAAGGCAAACCGGAGTTAACCCTTTACCGTCCGTATGCGGCTCGCGCGATGGAAGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGATTTGCGGTCAGTGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTAAATTCCCGTGGGATGACGGCATGAAAGTAGaaa                                                                                                                                                       >  1:220559/1‑250 (MQ=255)                                                                                          gTCAACAACTTAGGTTGTGCCGATTGCCATAACACCGCCTCTCCAGAGTTCGCCAAAGGCAAACCGGAGTTAACCCTTTCCCGTCCGTATGCGGCTCGCGCGATGGAAGCCATTGGTAAACCTTTTGAGCAAGCCGGACGTTTCGACCAGCAATCGATGATTTGCGGTCAGTGCCTTGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTAACTTCCCGTGGGATGACGGCATGAAAGTCGACAatat                                                                                                                                                   >  2:249810/1‑251 (MQ=255)                                                                                                        tggtgCCGATGGCCATAACACCGCCTCTCCAGAGTTCGCCAAAGGCAAACCGGAGTTAACCCTTTCCCGTCCGTATGCGGCTCGCGCGATGGAAGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGATTTGCGGTCAGTGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTAAATTCCCGTGGGATGACGGCATGAAAGTCGAAAATATGGAGCAGTATTAc                                                                                                                                      <  1:340633/248‑1 (MQ=255)                                                                                                                         ccacCGCCTCTCCAGAGTTCGCCAAAGGCAAACCGGAGTTAACCCTTTCCCGTCCGTATGCGGCTCGCGCGATGGAAGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGATTTGCGGTCAGTGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTAAATTCCCGTGGGATGACGGCATGAAAGTCGAAAATATGGAGCAGTATTACGACAAAATTGCCTTctct                                                                                                                    <  2:457589/250‑1 (MQ=255)                                                                                                                            ccGCCTCTCCAGAGTTCGCCAAAGGCAAACCGGAGTTAACCCTTTCCCGTCCGTATGCGGCTCGCGCGATGGAAGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGATTTGCGGTCAGTGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTAAATTCCCGTGGGATGACGGCATGAAAGTCGAAAATATGGAGCAGTATTACGACAAAATTGCCTTCTCTGAc                                                                                                                 <  1:2023/251‑1 (MQ=255)                                                                                                                                      agagGTCGTCAAAGGCAAACCGGAGTTAACCCTTGCCCCTCCGTATGCGGCTCGCGCGATGGAAGCGATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGATTTGCGGTCAGTGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTAAATTCCCGTGGGATGACGGCATGAAAGTCGAAAATATTGAGCAGTATTACGACAAAATTGCCTTCTCTTACTGGACTAACt                                                                                                       <  2:164356/251‑1 (MQ=255)                                                                                                                                             gCCAAAGGCAAACCGGAGTTAACCCTTTCCCGTCCGTAGGCGGCTCGCGCGATGGAAGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGATTTGCGGTCAGTGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTAAATTCCCGTGGGATGACGGCATGAAAGTCGAAAATATGGAGCAGTATTACGACAAAATTGCCTTCTCTGACTGGACTAACTCCCTGTc                                                                                                <  2:391510/251‑1 (MQ=255)                                                                                                                                                aaaGGCAAACCGGAGTTAACCCTTTCCCGTCCGTATGCGGCTCGCGCGATGGAAGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGATTTGCGGTCAGTGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTAAATTCCCGTGGGATGACGGCATGAAAGTCGAAAATATGGAGCAGTATTACGACAAAATTGCCTTCTCTGACTGGACTAACTCCCTGTCGa                                                                                              >  1:392707/1‑250 (MQ=255)                                                                                                                                                  aggccaaacggattttactcgttCCCGTCCGTATGCGGTTCGCGCTATGGAAGCCATTGGTAAACCTTATGAGACAGCCGGACGTTTCGACCAGCAATCGATGATTTGCGGTCTGTGCCATGTGGAGTATTACTGCGACAGCAAAAACAAAGCGGTTAAATTCCCGTGGGATGACGGCATGAAAGTCGAAAATATGGAGCAGTATTACGACAAAATTGCCTTCTCTGACTGGACTAACTCCCTGTCGaaaa                                                                                           <  2:352569/230‑1 (MQ=255)                                                                                                                                                   ggCAAACCGGAGTTAACCCTTTCCCGTCCGTATGCGGCTCGCGCGATGGAAGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGATTTGCGGTCAGTGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTAAATTCCCGTGGGATGACGGCATGAAAGTCGAAAATATGGAGCAGTATTACGACa                                                                                                                                  <  2:395083/211‑1 (MQ=255)                                                                                                                                                   ggCAAACCGGAGTTAACCCTTTCCCGTCCGTATGCGGCTCGCGCGATGGAAGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGATTTGCGGTCAGTGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTAAATTCCCGTGGGATGACGGCATGAAAGTCGAAAATATGGAGCAGTATTACGACa                                                                                                                                  >  1:395083/1‑211 (MQ=255)                                                                                                                                                      aaaCCGGAGTTAACCCTTTCCCGTCCGTATGCGGCTCGCGCGATGGAAGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGATTTGCGGTCAGTGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTAAATTCCCGTGGGATGACGGCATGAAAGTCGAAAATATGGAGCAGTATTACGACAAAATTGCCTTCTCTGACTGGACTAACTCCCTGTCGAAAACGcc                                                                                       <  1:168696/251‑1 (MQ=255)                                                                                                                                                         ccGGAGTTAACCCTTTCCCGTCCGTATGCGGCTCGCGCGATGGAAGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGATTTGCGGTCAGTGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTAAATTCCCGTGGGATGACGGCATGAAAGTCGAAAATATGGAGCAGTATTACGACAAAATTGCCTTCTCTGACTGGACTAACTCCCTGTCGAAAACGCCAAt                                                                                    <  1:42163/251‑1 (MQ=255)                                                                                                                                                                tAACCCTTTCCCGTCCGTATGCGGCTCGCGCGATGGAAGCCATTGGTAACCCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGATTTGCGGTCAGTGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTAAATTCCCGTGGGATGACGGCATGAAAGTCGAAAATATGGAGCAGTATTACGACAAAATTGCCTTCTCTGACTGGACTAACTCCCTGTCGAAAACGCCAATGCTGaa                                                                              >  2:241567/1‑250 (MQ=255)                                                                                                                                                                  aCCCTTTCCCGTCCGTATGCGGCTCGCGCGATGGAAGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGATTTGCGGTCAGTGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTAAATTCCCGTGGGATGACGGCATGAAAGTCGAAAATATGGAGCAGTATTACGACAAAATTGCCTTCTCTGACTGGACTAACTCCCTGTCGAAAACGCCAATGCTGAAAgc                                                                           <  1:302/251‑1 (MQ=255)                                                                                                                                                                       ttCCCGTCCGTATGCGGTTCGCGCGATGTAAGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGATTTGCGGTCAGTGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTAAATTCCCGTGTGATGACGGCATGAAAGTCGACAATATGGAGCAGTATTACGACAAAATTGCCTTCTCTGACTGGACTAACTCCCTGTCGAAAACGCCAATTCTGAAAGCgcag                                                                       <  1:386402/250‑1 (MQ=255)                                                                                                                                                                        tCCCGTCCGTATGCGGCTCGCGCGATGGAAGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGATTTGCGGTCAGTGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTAAATTCCCGTGGGATGACGGCATGAAAGTCGAAAATATGGAGCAGTATTACGACAAAATTGCCTTCTCTGACTGGACTAACTCCCTGTCGAAAACGCCAATGCt                                                                                 <  2:349486/239‑1 (MQ=255)                                                                                                                                                                        tCCCGTCCGTATGCGGCTCGCGCGATGGAAGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGATTTGCGGTCAGTGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTAAATTCCCGTGGGATGACGGCATGAAAGTCGAAAATATGGAGCAGTATTACGACAAAATTGCCTTCTCTGACTGGACTAACTCCCTGTCGAAAACGCCAATGCt                                                                                 >  1:349486/1‑239 (MQ=255)                                                                                                                                                                                                     aGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGATTTGCGGTCAGTGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTAAATTCCCGTGGGATGACGGCATGAAAGTCGAAAATATGGAGCAGTATTACGACAAAATTGCCTTCTCTGACTGGACTAACTCCCTGTCGAAAACGCCAATGCTGAAAGCGCAGCACCCGGAATATGAAACCTGGACAGCGGGc                                         >  1:251900/1‑250 (MQ=255)                                                                                                                                                                                                             gTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGATTTGCGGTCAGTGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTAAATTCCCGTGGGATGACGGCATGAAAGTCGAAAATATGGAGCAGTATTACGACAAAATTGCCTTCTCTGACTGGACTAACTCCCTGTCGAAAACGCCAATGCTGAAAGCGCAGCACCCGGAATATGAAACCTGGACAGCGGGCATTCACGGt                                <  1:241567/251‑1 (MQ=255)                                                                                                                                                                                                                   cTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGATTTGCGGTCAGTGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTAAATTCCCGTGGGATGACGGCATGAAAGTCGAAAATATGGAGCAGTATTACGACAAAATTGCCTTCTCTGACTGGACTAACTCCCTGTCGAAAACGCCAATGCTGAAAGCGCAGAACCCGGAATATGAAACCTGGACAGCGGGCATTCACGGTAAAaac                          >  1:38207/1‑251 (MQ=255)                                                                                                                                                                                                                     tttGAGAATGCCGGACGTTTCGACCAGCAATCGATGATTTGCGGTCAGTGCCATGTGGAGTACTACTTCGACGGCAAAAACAAAGCGGTTAAATTACCGTGGGAAGACGGCATGAAAGTCGAAAATATGGCGCAGTATTACGACAAAATTGACATCTCTGACTGGACTAACTCCCTGTCGAAAACGCCAATGCTGAAAGCGAAGCACCCGGAATATGAAAGCAGGACAGAGGGCATACCAGGTAAAaaca                         >  2:383017/1‑250 (MQ=255)                                                                                                                                                                                                                         agaAAGCCGGACGTTTCGACCAGCAATCGATGATTTGCGGTCAGTGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTAAATTCCCGTGGGATGACGGCATGAAAGTCGAAAATATGGAGCAGTATTACGACAAAATTGCCTTCTCTGACTGGACTAACTCCCTGTCGAAAACGCCAATGCTGAAAGCGCAGCACCCGGAATATGAAACCTGGACAGCGGGCATTCACGGTAAAAACAACGTg                    >  1:44034/1‑251 (MQ=255)                                                                                                                                                                                                                               ccGGACGTTTCGACCAGCAATCGATGATTTGCGGTCAGTGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTAAATTCCCGTGGGATGACGGCATGAAAGTCGAAAATATGGAGCAGTATTACGACAAAATTGCCTTCTCTGACTGGACTAACTCCCTGTCGAAAACGCCAATGCTGAAAGCGCAGCACCCGGAATATGAAACCTGGACAGCGGGCATTCACGGTAAAAACAACGTGACCTGt              <  1:249810/251‑1 (MQ=255)                                                                                                                                                                                                                                  gACGTTTCGACCAGCAATCGATGATTTGCGGTCAGTGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTAAATTCCCGTGGGATGACGGCATGAAAGTCGAAAATATGGAGCAGTATTACGACAAAATTGCCTTCTCTGACTGGACTAACTCCCTGTCGAAAACGCCAATGCTGAAAGCGCAGCACCCGGAATATGAAACCTGGACAGCGGGCATTCACGGTAAAAACAACGTGACCTGTATc           <  1:347445/251‑1 (MQ=255)                                                                                                                                                                                                                                     gTTTCGACCAGCAATCGATGATTTGCGGTCAGTGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTAAATTCCCGTGGGATGACGGCATGAAAGTCGAAAATATGGAGCAGTATTACGACAAAATTGCCTTCTCTGACTGGACTAACTCCCTGTCGAAAAc                                                                                          >  2:214485/1‑169 (MQ=255)                                                                                                                                                                                                                                     gTTTCGACCAGCAATCGATGATTTGCGGTCAGTGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTAAATTCCCGTGGGATGACGGCATGAAAGTCGAAAATATGGAGCAGTATTACGACAAAATTGCCTTCTCTGACTGGACTAACTCCCTGTCGAAAAc                                                                                          <  1:214485/169‑1 (MQ=255)                                                                                                                                                                                                                                       ttCGACCAGCAATCGATGATTTGCGGTCAGTGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTAAATTCCCGTGGGATGACGGCATGAAAGTCGAAAATATGGAGCAGTATTACGACAAAATTGCCTTCTCTGACTGGACTAACTCCCTGTCGAAAACGCCAATGCTGAAAGCGCAGCACCCGGAATATGAAACCTGGACAGCGGGCATTCACGGTAAAAACAACGTGACCTGTATCGACt       <  2:58054/250‑1 (MQ=255)                                                                                                                                                                                                                                         cGACCAGCAATCGATGATTTGCGGTCAGTGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTAAATTCCCGTGGGATGACGGCATGAAAGTCGAAAATATGGAGCAGTATTACGACAAAATTGCCTTCTCTGACTGGACTAACTCCCTGTCGAAAACGCCAATGCTGAAAGCGCAGCACCCGGAATATGAAACCTGGACAGCGGGCATTCACGGTAAAAACAACGTGACCTGTATCGACTGcc    >  1:28017/1‑251 (MQ=255)                                                                                                                                                                                                                                            ccAGCACTCGATGATTTGCGGTCAGTGCCATGTGGAGCATTACTTCGACGGCAAAAACAAAGCGGTTAACTTCCCGTGGGATGACGGCATGAAAGTCGAAAATATGGAACAGTATTACGACCAAATTGCCTTCTCTGACTGGACTAACTCCATGTCGAAAACGCCAATGCTGAAAGCGCAGCACCCGGCATATGAAACCTGGACAGCGGGCATTCACGGTAAAAACAACGTAGCCTGTATCGACTGCCat  >  2:275981/1‑250 (MQ=255)                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                             TAAAAGCCCGGATGTGGCGCGTCTGATCCAGAAAGACGGCGAAGATGGCTACTTCCACGG‑TAAATGGGCGCGCGGCGGTCCGGAAATCGTCAACAACTTAGGTTGTGCCGATTGCCATAACACCGCCTCTCCAGAGTTCGCCAAAGGCAAACCGGAGTTAACCCTTTCCCGTCCGTATGCGGCTCGCGCGATGGAAGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGTGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTAAATTCCCGTGGGATGACGGCATGAAAGTCGAAAATATGGAGCAGTATTACGACAAAATTGCCTTCTCTGACTGGACTAACTCCCTGTCGAAAACGCCAATGCTGAAAGCGCAGCACCCGGAATATGAAACCTGGACAGCGGGCATTCACGGTAAAAACAACGTGACCTGTATCGACTGCCAT  >  NC_000913/4288138‑4288621

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑