Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,283,465	G→A	P480S (CCG→TCG)	actP ←	acetate/glycolate:cation symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,283,465	0	G	A	95.8%	138.7 / ‑4.1	48	P480S (CCG→TCG)	actP	acetate/glycolate:cation symporter
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base A (29/17);  total (30/18)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.56e-01

TCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCGGGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCCCAGCCAGCCACCCATCATCGCGCCACGCGTGGTCAGTTTCGACCAGTACATAGAAAGCAGAATGATCGGGAAGTTACAGCTCGCCGCGATGGCAAACGCCAGCCCCACCATAAAGGCGATGTTCTGATTCTCAAACAGCACGCCGAGGATAATCGCAATCACGCCGAGGATCAGTACGGTGATTTTTGATACCCGCAGCTCTTCACGTTC  >  NC_000913/4283221‑4283711                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                       ccccagggcAGACGACCGGGGAAGGGGGGAGATTAAGGCGCTCGGCCGTTCGAAACGCCAAAGCCGGTCGGGGGGGGGATAAACTGCGCGCGGAACAGTTCACGCTCAGGCGCGCCTTCCGCTGATTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGGACCCAAATCGTCGAGCCGAg                                                                                                                                                                                                                                                  <  2:416375/243‑1 (MQ=255)    ccGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCGAGCCGAGGat                                                                                                                                                                                                                                               >  1:182355/1‑251 (MQ=255)                  ggggAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTATGGGAGCGGATAAACTGCGCGAGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCCCGGTGATAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCAAAATCGTCGAGCCGAGGATAATCAGTACTACTg                                                                                                                                                                                                                                 >  2:436129/1‑251 (MQ=255)                           gcgagaTTCATGCGCGAGGCCTTGCTCAACGAAAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTACGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAACAGCACCTGGTATTCATACGGGAAGATTGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCGAGCCGAGGATCATCAGTACAACTGCGGTAATc                                                                                                                                                                                                                         >  2:91917/3‑250 (MQ=255)                                                    tCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCCCGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCAACGGTGATAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCGAGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCCCAGCCAGCCACCCATCATcgc                                                                                                                                                                                               >  1:427806/1‑251 (MQ=255)                                                      aaCGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGAACCCAAATCGTCGAGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCCCAGCCAGCCACCCATCATCgcgc                                                                                                                                                                                             >  1:317728/1‑251 (MQ=255)                                                               gCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCGAGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCCCAGCCAGCCACCCATCATCGCGCCACGCGTgg                                                                                                                                                                                    >  1:68387/1‑251 (MQ=255)                                                                          ggCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTAATCGGTGGCCGAGAAGAACCATATGCCGATGAAGGCCACGGTGATAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCGAGCCGAGGATCATCAGTACTACTGCGGGAATCAGCCCCAGCCAGCCACCCATCATCGCGCCACGCGTGGGCAGGTTCGAc                                                                                                                                                                         <  2:427806/250‑1 (MQ=255)                                                                             ggATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGCCGGGTATTCATACGGGATGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCGAGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCCCAGCCAGCCACCCATCATCGCGCCACGCGTGGTCAGTTTCGAcc                                                                                                                                                                        >  1:223169/1‑249 (MQ=255)                                                                                      gcgcgcGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCGAGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCCCAGCCAGCCACCCATCATCGCGCCACGCGTGGTCAGTTTCGACCAGTACATAg                                                                                                                                                               <  1:344598/249‑1 (MQ=255)                                                                                            ggAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGCCGGGTATTCATACGGGATGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCGAGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCCCAGCCAGCCACCCATCATCGCGCCACGCGTGGTCAGTTTCGACCAGTACATAGAAAGCAGa                                                                                                                                                       <  2:223169/251‑1 (MQ=255)                                                                                               aCAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCGAGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCCCAGCCAGCCACCCATCATCGCGCCACGCGTGGTCAGTTTCGACCAGTACATAGAAAGCAGAATg                                                                                                                                                    >  1:270492/1‑251 (MQ=255)                                                                                                        gctcacgcGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCGAGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCCCAGCCAGCCACCCATCATCGCGCCACGCGTGGTCAGTTTCGTCCAGTACATAGAAAGCAGAATGATCGGGa                                                                                                                                             >  2:110569/1‑249 (MQ=255)                                                                                                         ctcacgcGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCGAGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCCCAGCCAGCCACCCATCATCGCGCCACGCGTGGTCAGTTTCGACCAGTACATAGGAAGCAGAATGATCGGGAAGt                                                                                                                                          >  1:483150/1‑251 (MQ=255)                                                                                                                gcgcCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCGAGCCGAGGATCCTCAGTACTACTGCGGTAATCAGCCCCAGCCAGCCACCCATCATCGCGCCACGCGTGGTCAGTTTCGACCAGGACATAGAAAGCAGAATGATCGGGAAGTTACAGCt                                                                                                                                   >  2:275793/1‑251 (MQ=255)                                                                                                                 cgcCTTCCGCTGAGTTATCGGTGGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCGAGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCCCAGCCAGCCACCCATCATCGCGCCACGCGTGGTCAGTTTCGACCAGTACATAGAAAGCAGAATGATCGGGAAGTTACAGCTc                                                                                                                                  <  2:182355/251‑1 (MQ=255)                                                                                                                  gcCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCGGGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCCCAGCCAGCCACCCATCATCGCGCCACGCGTGGTCAGTTTCGACCAGTACATAGAAAGCAGAATGATCGGGAAGTTAc                                                                                                                                       >  1:303915/1‑245 (MQ=255)                                                                                                                                   cGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCGAGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCCCAGCCAGCCACCCATCATCGCGCCACGCGTGGTCAGTTTCGACCAGTACATAGAAAGCAGAATGATCGGGAAGTTACAGCTCGCCGCGATGGCCAACGcc                                                                                                                >  2:77044/1‑251 (MQ=255)                                                                                                                                   cGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCGAGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCCCAGCCAGCCACCCATCATCGCGCCACGCGTGGTCAGTTTCGACCAGTACATAGAAAGCAGAATGATCGGGAAGTTACAGCTCGCCGCGATGGCAAACGcc                                                                                                                <  1:119518/251‑1 (MQ=255)                                                                                                                                        gCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGCCGGGGATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCGAGCCGGGGATCATCAGTACTACTGCGGTAATCAGCCCCAGCCAGCCACCCATCATCGCGCCAAGCGTGGTCAGTTTCGACCAGTACATAGAAAGCAGAATGATCGGGAAGTTACAGCTCGCCGCGATGGCAACCGCCAGccc                                                                                                           >  2:375676/1‑251 (MQ=255)                                                                                                                                              aagaaCCAGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCGAGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCCCAGCCAGCCACCCATCATcg                                                                                                                                                                                                <  2:90050/160‑1 (MQ=255)                                                                                                                                              aagaaCCAGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCGAGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCCCAGCCAGCCACCCATCATcg                                                                                                                                                                                                >  1:90050/1‑160 (MQ=255)                                                                                                                                                           cGAGGAATGCCACGGTGATAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCGAGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCCCAGCCAGCCACCCATCATCGCGCCACGCGTGGTCAGTTTCGACCAGTACATAGAAAGCAGAATGATCGGGAAGTTACAGCTCGCCGCGATGGCAAACGCCAGCCCCACCATACAGGCGAGGTTc                                                                                        >  1:235336/1‑251 (MQ=255)                                                                                                                                                            gAGGAATGCCACGGTGATAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCGAGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCCCAGCCAGCCACCCATCATCGCGCCACGCGTGGTCAGTTTCGACCAGTACATAGAAAGCAGAATGATCGGGAAGTTACAGCTCGCCGCGATGGCAAACGCCAGCCCCACCATAAAGGCGATGTTCt                                                                                       >  1:374098/1‑251 (MQ=255)                                                                                                                                                             aGGAATGCCACGGTGATAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCGAGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCCCAGCCAGCCACCCATCATCGCGCCACGCGTGGTCAGTTTCGACCAGTACATAGaaa                                                                                                                                                            <  2:99813/181‑1 (MQ=255)                                                                                                                                                             aGGAATGCCACGGTGATAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCGAGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCCCAGCCAGCCACCCATCATCGCGCCACGCGTGGTCAGTTTCGACCAGTACATAGaaa                                                                                                                                                            >  1:99813/1‑181 (MQ=255)                                                                                                                                                                  tGCCACGGTGATAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCGAGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCCCAGCCAGCCACCCATCATCGCGCCACGCGTGGTCAGTTTCGACCAGTACATAGAAAGCAGAATGATCGGGAAGTTACAGCTCGCCGCGATGGCAAACGCCAGCCCCACCATAAAGGc                                                                                               <  1:169924/237‑1 (MQ=255)                                                                                                                                                                  tGCCACGGTGATAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCGAGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCCCAGCCAGCCACCCATCATCGCGCCACGCGTGGTCAGTTTCGACCAGTACATAGAAAGCAGAATGATCGGGAAGTTACAGCTCGCCGCGATGGCAAACGCCAGCCCCACCATAAAGGc                                                                                               >  2:169924/1‑237 (MQ=255)                                                                                                                                                                     cACGGTGATAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCGAGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCCCAGCCAGCCACCCATCATCGCGCCACGCGTGGTCAGTTTCGACCAGTACATAGAAAGCAGAATGGTCGGGAAGTTACAGCTCGCCGCGATGGCAAACGCCAGCCCCACCATAAAGGCGATGTTCTGATTCTCa                                                                               >  2:400930/1‑250 (MQ=255)                                                                                                                                                                           gATAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCGAGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCCCAGCCAGCCACCCATCATcgc                                                                                                                                                                                               <  1:102628/132‑1 (MQ=255)                                                                                                                                                                           gATAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCGAGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCCCAGCCAGCCACCCATCATCGCGCCACGCGTGGTCAGTTTCGACCAGTACATAGAAAGCAGAATGATCGGGAAGTTCCAGCTCGCCGCGATGGCAAACGCCAGCCCCACCATAAAGGCGATGTTCTGATTCTCAAACAGc                                                                         >  2:423137/1‑250 (MQ=255)                                                                                                                                                                           gATAGAGAACAGCGCCGGGGATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCGAGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCCCAGCCAGCCACCCATCATcgc                                                                                                                                                                                               >  2:102628/1‑132 (MQ=255)                                                                                                                                                                                         ccGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCGAGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCCCAGCCAGCCACCCATCATCGCGCCACGCGTGGTCAGTTTCGACCAGTACATAGAAAGCAGAATGATCGGGAAGTTACAGCTCGCCGCGATGGCAAACGCCAGCCCCACCATAAAGGCGATGTTCTGATTCTCAAACAGCACGCCGAGGATAATc                                                          >  2:286215/1‑251 (MQ=255)                                                                                                                                                                                                ttCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCGAGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCCCAGCCAGCCACCCATCATCGCGCCACGCGTGGTCAGTTTCGACCAGTACATAGAAAGCAGAATGATCGGGAAGTTACAGCTCGCCGCGATGGCAACCGCCAGCCCCACCATAAAGGCGATGTTCTGATTCTCAAACAGCACGCCGAGGATAATCGCAATc                                                    >  1:434068/1‑250 (MQ=255)                                                                                                                                                                                                      cGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCGAGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCCCAGCCAGCCACCCATCATCGCGCCACGCGTGGTCAGTTTCGACCAGTACATAGAAAGCAGAATGATCGGGAAGTTACAGCTCGCCGCGATGGCAAACGCCAGCCCCACCATAAAGGCGATGTTCTGATTCTCAAACAGCACGCCGAGGATAATCGCAATCACGCCg                                              >  1:429318/1‑250 (MQ=255)                                                                                                                                                                                                               ggCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCGAGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCCCAGCCAGCCACCCATCATCGCGCCACGCGTGGTCAGTTTCGACCAGTACATAGAAAGCAGAATGATCGGGAAGTTACAGCTCGCCGCGATGGCAAACGCCAGCCCCACCATAAAGGCGATGTTCTGATTCTCAAACAGCACGCCGAGGATAATCGCAATCACGCCGAGGATCAGTa                                    <  1:148484/251‑1 (MQ=255)                                                                                                                                                                                                               ggCTTTTTCGTGACCAAGGATCTGTACCCAAATCGGCGAGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCCCAGCCAGCCACCCATCATCGCGCCACGCGTGGTCAGTTTCGACCAGTACATAGAAAGCAGAATGATCGGGAAGTTACAGCTCGCCGCGATGGCAAACGCCAGCCCCACCATAAAGGCGATGTTCTGATTCTCAAACAGCACGCCGAGGATAATCGCAATCACGCCGAGGATCAGt                                     <  1:286215/250‑1 (MQ=255)                                                                                                                                                                                                                  tttttCGTGACCAAGGATCTGTACCCAAATCGTCGAGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCCCAGCCAGCCACCCATCATCGCGCCACGCGTGGTCAGTTTCGACCAGTACATAGAAAGCAGAATGATCGGGAAGTTACAGCTCGCCGCGATGGCAAACGCCAGCCCCACCATAAAGGCGATGTTCTGATTCTCAAACAGCACGCCGAGGATAATCGCAATCACGCCGAGGATCAGTACgg                                 >  1:157536/1‑251 (MQ=255)                                                                                                                                                                                                                    tttCGTGACCAAGGATCTGTACCCAAATCGTCGAGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCCCAGCCAGCCACCCATCATCGCGCCACGCGTGGTCAGTTTCGACCAGTACATAGAAAGCAGAATGATCGGGAAGTTACAGCTCGCCGCGATGGCAAACGCCAGCCCCACCATAAAGGCGATGTTCTGATTCTCAAACAGCACGCCGAGGATAATCGCAATCACGCCGAGGATCAGTACGGt                                <  1:275039/250‑1 (MQ=255)                                                                                                                                                                                                                           cccatggATCTGTACCCAAGCCGTCGGGACGAGGATCATCAGTACTACTGCGGTAAGCAGCCCCAGCCAGCCACCCATCATCGCGCCACGCGTGGTCAGTTTCGACCAGAACATAGAAAGCAGAATGATCGGGAAGTTACAGCGCGCCGCGATGGCAAACGCCAGCCCCACCATAAAGGCGATGTTCTGATTCTCAAACAGCACGCCGAGGATAATCGCAATCACGCCGAGGATCAGTACGGTGATTTTTg                        <  2:91023/246‑1 (MQ=255)                                                                                                                                                                                                                             cAAGGATCTGTACCCAAATCGTCGAGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCCCAGCCAGCCACCCATCATCGCGCCACGCGTGGTCAGTTTCGACCAGTACATAGAAAGCAGAATGATCGGGAAGTTACAGCTCGCCGCGATGGCAAACGCCAGCCCCACCATAAAGGCGATGTCCTGATTCTCAACCAGCACGCCGCGGAGAATCGCAATCACGCCGAGGATAAGTACGGTGATTTTTggt                      >  2:71702/1‑249 (MQ=255)                                                                                                                                                                                                                                ggATCTGTACCCAAATCGTCGAGCCGGGGATCATCAGTACTACTGCGGTAATCAGCCCCAGCCAGCCACCCATCATCGCGCCACGCGTGGTCAGTTTCGACCAGTACATAGAAAGCAGAATGATCGGGAAGTTACAGCTCGCCGCGATGGCAAACGCCAGCCCCACCATAAAGGCGATGTTCTGATTCTCAAACAGCACGCCGAGTATAATCGCAATCACGCCGAGGATCAGTACGGTGATTTTTGATAcc                   <  1:375676/251‑1 (MQ=255)                                                                                                                                                                                                                                agatatGTACACAAATCGTCGAGCCGCGGATCATCCGGACTCCTGCAGTAAACAGCCACAGCCAGCAACCCATCATCGCGCCACGCGTGGTCAGTTTCGACCAGTACATAGAAAGCAGAATGATCGGGAAGCTACAGCTCGCCGCGGTGGCAAACGCCAGCCCCACCATACCGGCGATGTTCTGATTCTCAAACAGCACGCCCAGGGCAATCGCCAACACGCCGGGGATCACTACGGGGAGTTTTGATAcc                   >  2:207709/6‑251 (MQ=255)                                                                                                                                                                                                                                 gATCTGTACCCAAATCGTCGAGCCGAGGATCATCCGTACTACTGCGGTAATCAGCCCCAGCCAGCCACCCATCATCGCGCCACGCGTGGTCAGTTTCGACCAGTACATAGAAAGCAGAATGATCGGGAAGTTACAGCTCGCCGCGATGGCAAACGCCAGCCCCACCATAAAGGCGATGTTCTGATTCTCAAACAGCACGCCGAGGATAATCGCAATCACGCCGAGGATCAGTACGGTGATTTTTGATAccc                  >  1:354531/1‑251 (MQ=255)                                                                                                                                                                                                                                     tGTACCCAAATCGTCGAGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCCCAGCCAGCCACCCATCATCGCGCCACGCGTGGTCAGTTTCGACCAGTACATAGAAAGCAGAATGATCGGGAAGTTACAGCTCGCCGCGATGGCAAACGCCAGCCCCACCATAAAGGCGCTGTTCTGATTCCCAAACAGCACGCCGAGGATAATCGCAATCACGCCGAGGATCCGGACGGTGATTTTTGAAACCCGccg              >  2:159298/1‑249 (MQ=255)                                                                                                                                                                                                                                           cAAATCTTCGAGCCGAGGATCATCAGTACTACTGCTGTAATCAGCCCCAGCCAGCCACCCATCATCGCGCCACGCGTGGACAGTTTCGCCCAGTACATAGAAAGCAGAATGATCGGGAAGTTACAGCTCGCCGCGATGGCAAACGCCAGCCCCACCATAAAGGCGATGTTCTGATTCTCAAACAGCACGCCGAGGATAATCGCAATCACGCCGAGGATCAGTTCGGTGATTTTTGATTCCCGCAGCTCTTc        <  1:297703/251‑1 (MQ=255)                                                                                                                                                                                                                                             aatggtcgAGCCTAGGATCATCAGTACTACTGCGTGAATCATCCCATGCCAGCCCCCCATCATCGCGACACGCTGGGTCAGTTTCGACCAGTACATAGAAAGCAGAATGATCGGGAAGTGACAGCACGCCGGGATGGAAAACGCCAGCCCCACCATAAATGCGATGTTCTGATTCTCAAACAGCACGCCGAGGATAATCTCAATCACGCCGAGGATCAGTACGGTGATTTGTGATACCCGCAGctctcctt      <  2:268141/247‑5 (MQ=255)                                                                                                                                                                                                                                                 gtcgAGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCCCAGCCAGCCACCCATCATCGCGCCACGCGTGGTCAGTTTCGACCAGTACATAGAAAGCAGAATGATCGGGAAGTTACAGCTCGCCGCGATGGCAAACGCCAGCCCCACCATAAAGGCGATGTTCTGATTCTCAAACAGCACGCCGAGGATAATCGCAATCACGCCGAGGATCAGTACGGTGATTTTTGATACCCGCAGCTCTTCACGTTc  <  1:77044/251‑1 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                       TCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCGGGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCCCAGCCAGCCACCCATCATCGCGCCACGCGTGGTCAGTTTCGACCAGTACATAGAAAGCAGAATGATCGGGAAGTTACAGCTCGCCGCGATGGCAAACGCCAGCCCCACCATAAAGGCGATGTTCTGATTCTCAAACAGCACGCCGAGGATAATCGCAATCACGCCGAGGATCAGTACGGTGATTTTTGATACCCGCAGCTCTTCACGTTC  >  NC_000913/4283221‑4283711

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑