Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,281,093	G→A	A372T (GCG→ACG)	yjcE →	putative transporter YjcE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,281,093	0	G	A	100.0%	206.3 / NA	66	A372T (GCG→ACG)	yjcE	putative transporter YjcE
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (26/40);  total (26/40)

GTCTGCAGCTGCCGGGTATTCTGGAGACGTCGCTGATGGCAGCAGAAATCGACCCTAACGTCGAAATCTGGATGCTGTTTACCAATATTATTCTGATATATGCGGCGCTGATGCTGGTCCGTTTCGGCTGGCTGTGGACGATGAAAAAGTTCAGCAACCGCTTCCTGAAGAAGAAGCCGATGGAGTTTGGTTCGTGGACCACACGAGAAATCCTGATCGCGTCTTTCGCCGGGGTGCGTGGGGCGATCACTCTGGCCGGTGTGCTCTCTATCCCGCTGCTCTTGCCGGATGGTAACGTCTTCCCGGCGCGCTATGAGCTGGTGTTCCTGGCGGCTGGCGTCATTCTCTTCTCGCTGTTTGTCGGCGTGGTGATGTTGCCTATTCTGCTACAACACATTGAAGTCGCGGATCATTCGCAACAATTGAAAGAGGAACGTATTGCGCGAGCGGCAACGGCAGAAGTGGCGATTGTGGCGATCCAGAAAATG  >  NC_000913/4280851‑4281338                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                      ggcggCAGCTGCCGGGTATTCTGGAGACGTCGCTGATGGCGGCAGAAAGCGACCCTAACGTCGAAATCTGGATGCTGTTGTCCAATATTATTCTGATATATGCGGCGCTGATGCTGGTCCGTTGCGGCTGGCTGTGGACGATGAAAAAGTTCAGCAACCGCTTCCTGAAGAAGAAGCCGATGGAGGTTGGTTCGTGGACCACACGAGAAATCCTGACCGCGTCTTTCGCTGGGGTGCGTGGGACGATccct                                                                                                                                                                                                                                               <  2:298308/247‑4 (MQ=255)                            cGTCGCTGATGGCAGCAGAAATCGACCCTAACGTCGAAATCTGGATGCTGTTTACCAATATTATTCTGATATATGCGGCGCTGATGCTGGTCCGTTTCGGCTGGCTGTGGACGATGAAAAAGTTCAGCAACCGCTTCCTGAAGAAGAAGCCGATGGAGTTTGGTTCGTGGACCACACGAGAAATCCTGATCGCGTCTTTCGCCGGGGTGCGTGGGACGATCACTCTGGCCGGTGTGCTCTCTATCCCgctg                                                                                                                                                                                                                    <  2:292694/251‑1 (MQ=255)                                     gggCAGCAGAAATCGACCCTAACGTCGAAATCTGGATGCGGTTTACCAATATTATTCTGATATATGCGGCGCTGATGCTGGTCCGTTTCGGCTGGCTGTGGACGATGAAAAAGTTCAGCAACCGCTTCCTGAAGAAGAAGCCGATGGAGTTTGGTTCGTGGACCACACGAGAAATCCTGATCGCGTCTTTCGCCGGGGTGCGTGGGACGATCACTCTGGCCGGTGTGCTCTCTATCCCGCTGCTCTTGCCg                                                                                                                                                                                                           <  2:298826/250‑1 (MQ=255)                                           cagAAATCGACCCTAACGTCGAAATCTGGATGCTGTTTACCAATATTATTCTGATATATGCGGCGCTGATGCTGGTCCGTTTCGGCTGGCTGTGGACGATGAAAAAGTTCAGCAACCGCTTCCTGAAGAAGAAGCCGATGGAGTTTGGTTCGTGGACCACACGAGAAATCCTGATCGCGTCTTTCGCCGGGGTGCGTGGGACGATCACTCTGGCCGGTGTGCTCTCTATCCCGCTGCTCTTGCCGGATGGt                                                                                                                                                                                                     >  1:280839/1‑251 (MQ=255)                                           aagAAATCGACCCTAACGTCGAAATCTGGAGGCTGTTTACCAATATTATTCTGATATATGCGGCGCTGATGCTGGTCCGTTTCGGCTGGCTGTGGACGATGAAAAAGTTCAGCAACCGCTTCCTGAAGAAGAAGCCGATGGAGTTTGGTTCGTGGACCACACGAGAAATCCTGATCGCGTCTTTCGCCGGGGTGCGTGGGACGATCACTCTGGCCGGTGTGCTCTCTATCCCGCTGCTCTTGCCGGATGGt                                                                                                                                                                                                     <  2:280839/250‑1 (MQ=255)                                                  cGACCCTAACGTCGAAATCTGGATGCTGTTTACCAATATTATTCTGATATATGCGGCGCTGATGCTGGTCCGTTTCGGCTGGCTGTGGACGATGAAAAAGTTCAGCAACCGCTTCCTGAAGAAGAAGCCGATGGAGTTTGGTTCGTGGACCACACGATAAATCCTGATCGCGTCTTTCGCCGGGGTGCGTGGGACGATCACTCTGGCCGGTGTGCTCTCTATCCCGCTGCTCTTGCCGGATGGTAACGTCt                                                                                                                                                                                              >  2:164321/1‑251 (MQ=255)                                                    aCCCTAACGTCGAAATCTGGATGCTGTTTACCAATATTATTCTGATATATGCGGCGCTGATGCTGGTCCGTTTCGGCTGGCTGTGGACGATGAAAAAGTTCAGCAACCGCTTCCTGAAGAAGAAGCCGATGGAGTTTGGTTCGTGGACCACACGAGAAATCCTGATCGCGTCTTTCGCCGGGGTGCGTGGGACGATCACTCTGGCCGGTGTGCTCTCTATCCCGCTGCTCTTGCCGGATGGTAACGTCTTc                                                                                                                                                                                            <  1:389495/251‑1 (MQ=255)                                                    aCCCTAACGTCGAAATCTGGATGCTGTTTACCAATATTATTCTGATATATGCGGCGCTGATGCTGGTCCGTTTCGGCTGGCTGTGGACGATGAAAAAGTTCAGCAACCGCTTCCTGAAGAAGAAGCCGATGGAGTTTGGTTCGTGGACCACACGAGAAATCCTGATCGCGTCTTTCGCCGGGGTGCGTGGGACGATCACTCTGGCCGGTGTGCTCTCTATCCCGCTGCTCTTGCCGGATGGTAACGTCTTc                                                                                                                                                                                            >  1:194503/1‑251 (MQ=255)                                                            gTCGAAATCTGGATGCTGTTTACCAATATTATTCTGATATATGCGGCGCTGATGCTGGTCCGTTTCGGCTGGCTGTGGACGATGAAAAAGTTCAGCAACCGCTTCCTGAAGAAGAAGCCGCTGGAGTTTGGTTCGTGGACCACACGAGAAATCCTGATCGCGTCTTTCGCCGGGGTGCGTGGGACGATCACTCTGGCCGGTGTGCTCTCTATCCCGCTGCTCTTGCCGGATGGTACCGTCTTCCCGgcgcg                                                                                                                                                                                    >  2:412248/1‑251 (MQ=255)                                                                aaaTCTGGATGCTGTTTACCAATATTATTCTGATATATGCGGGGCTGATGCTGGTCCGTTTCGGCTGGCTGTGGACGATGAAAAAGTTCAGCAACCGCTTCCTGAAGAAGAAGCCGATGGAGTTTGGTTCGTGGACCACACGAGAAATCCTGATCGCGTCTTTCGCCGGGGTGCGTGGGACGATCACTCTGGCCGGTGTGCTCTCTATCCCGCTGCTCTTGCCGGATGGTAACGTCTTCCCGGCGCGCTAt                                                                                                                                                                                <  2:82019/251‑1 (MQ=255)                                                                    cgggATGCGGTTTACCAATATTAGTCTGATATATGCGGCGCTGAGGCTGGTCCGTTTCGGCTGGCTGTGGACGATGAAAAATTTCAGCAACCGCTTCCTGAAGAAGAAGCCGATGGAGTTTGGTTCGTGGACCACACGAGAAATCCTGATCGCGTCTTTCGCCGGGGTGCGTGGGACGATCACTCTGGCCGGTGTGCTCTCTATCCCGCTGCTCTTGCCGGATGGTAACGGCTTCCCGGCGCGCTATGAGc                                                                                                                                                                            <  2:249952/249‑1 (MQ=255)                                                                     gggATGCTGTTTACCAATATTATTCTGATAGATGCGGCGCTGATGCTGGTCCGTTTCGGCTGGTTGTGGACGATGAAAAAGTTCAGCAACCGCTTCCTGAAGAAGAAGCCGATGGAGTTTGGTTCGTGGACCACACGAGAAATCCTGATCGCGTCTTTCGCCGGGGTGCGTGGGACGATCACTCTGGCCGGTGTGCTCTCTATCCCGCTGCTCTTGCCGGATGGTAACGTCTTCCCGGCGCGCTATGAGCt                                                                                                                                                                           <  2:324618/250‑1 (MQ=255)                                                                        aTGCTGTTTACCAATATTATTCTGATATATGCGGCGCTGATGCTGGTCCGTTTCGGCTGGCTGTGGACGATGAAAAAGTTCAGCAACCGCTTCCTGAAGAAGAAGCCGATGGAGTTTGGTTCGTGGACCACACGAGAAATCCTGATCGCGTCTTTCGCCGGGGTGCGTGGGACGATCACTCTGGCCg                                                                                                                                                                                                                                        <  1:127184/187‑1 (MQ=255)                                                                        aTGCTGTTTACCAATATTATTCTGATATATGCGGCGCTGATGCTGGTCCGTTTCGGCTGGCTGTGGACGATGAAAAAGTTCAGCAACCGCTTCCTGAAGAAGAAGCCGATGGAGTTTGGTTCGTGGACCACACGAGAAATCCTGATCGCGTCTTTCGCCGGGGTGCGTGGGACGATCACTCTGGCCg                                                                                                                                                                                                                                        >  2:127184/1‑187 (MQ=255)                                                                        aTGCTGTTTACCAATATTATTCTGATATATGCGGCGCTGATGCTGGTCCGTTTCGGCTGGCTGTGGACGATGAAAAAGTTCAGCAACCGCTTCCTGAAGAAGAAGCCGATGGAGTTTGGTTCGTGGACCACACGAGAAATCCTGATCGCGTCTTTCGCCGGGGTGCGTGGGACGATCACTCTGGCCGGTGTGCTCTCTATCCCGCTGCTCTTGCCGGATGGTAACGTCTTCCCGGCGCGCTATGAGCTGgt                                                                                                                                                                        <  1:465873/251‑1 (MQ=255)                                                                         tGCTGTTTACCAATATTATTCTGATATATGCGGCGCTGATGCTGGTCCGTTTCGGCTGGCTGTGGACGATGAAAAAGTTCAGCAACCGCTTCCTGAAGAAGAAGCCGATGGAGTTTGGTTCGTGGACCACACGAGAAATCCTGATCGCGTCTTTCGCCGGGGTGCGTGGGACGATCACTCTGGCCGGTGTGCTCTCTATCCCGCTGCTCTTGCCGGATGGTAACGTCTTCCCGGCGCGCTATGAGCTGgtg                                                                                                                                                                       >  1:393273/1‑251 (MQ=255)                                                                          gCTGTTTACCAATATTATTCTGATATATGCGGCGCTGATGCTGGTCCGTTTCGGCTGGCTGTGGACGATGAAAAAGTTCAGCAACCGCTTCCTGAAGAAGAAGCCGATGGAGTTTGGTTCGTGGACCACACGAGAAATCCTGATCGCGTCTTTCGCCGGGGTGCGTGGGACGATCACTCTGGCCGGTGTGCTCTCTATCCCGCTGCTCTTGCCGGATGGTAACGTCTTCCCGGCGCGCTATGAGCTGgtgt                                                                                                                                                                      >  1:304147/1‑251 (MQ=255)                                                                          gCTGTTTACCAATATTATTCTGATATATGCGGCGCTGATGCGGGTCCGTTTCGGCTGGCTGTGGACGATGAAAAAGTTCAGCATCCGCGTCCTGAAGAAGAAGCCGATGGAGTTTGGTTCGTGGACCACACGAGAAATCCGGGTCGCGGCTTTCGCCGGGGTGCGTGGGACGATCACTCGTGCCTGTGTGCTCTCTATCCCGCTGCTCTTGCCGGATGGTAACGTCTTCCCGGCGTGCTATGAGCTGgtg                                                                                                                                                                       <  2:440462/250‑1 (MQ=255)                                                                               ttACCAATATTATTCTGATATATGCGGCGCTGATGCTGGTCCGTTTCGGCTGGCTGTGGACGATGAAAAAGTTCAGCAACCGCTTCCTGAAGAAGAAGCCGATGGAGTTTGGTTCGTGGACCACACGAGAAATCCTGATCGCGTCTTTCGCCGGGGTGCGTGGGACGATCACTCTGGCCGGTGTGCTCTCTATCCCGCTGCTCTTGCCGGATGGTAACGTCTTCCCGGCGCGCTCTGAGCTGGTGTTCCTg                                                                                                                                                                 >  1:434847/1‑251 (MQ=255)                                                                                  ccAATATTATTCTGATATATGCGGCGCTGATGCTGGTCCGTTTCGGCTGGCTGTGGACGATGAAAAAGTTCAGCAACCGCTTCCTGAAGAAGAAGCCGATGGAGTTTGGTTCGTGGACCACACGAGAAATCCTGATCGCGTCTTTCGCCGGGGTGCGTGGGACGATCACTCTGGCCGGTGTGCTCTCTATCCCGCTGCTCTTGCCGGATGGTAACGTCTTCCCGGCGCGCTATGAGCTGGTGTTCCTggcg                                                                                                                                                              >  1:277968/1‑251 (MQ=255)                                                                                  ccAATATTATTCGGATATATGCGGCGCTGAGGCTGGTCCGTTTCGGCTGGCTGTGGACGATGAAAAAGTGCAGCAACCGCTTCCTGAAGAAGAAGCCGATGGAGTTTGGTTCGTGGACCACACGAGAAATCCTGATCGCGTCTTTCGCCGGGGTGCGTGGGACGATCACTCTGGCCGGTGTGCTCTCTATCCCGCTGCTCTTGCCGGATGGTAACGTCTTCCCGGCGCGCTATGAGCTGGTGTTCCTggcg                                                                                                                                                              <  2:311616/251‑1 (MQ=255)                                                                                       atgtgtCTGATATATGCGGCGCTGGTGCTGGTTCGTTTCGTCGTGCGGTGGACGATGAAAAAGTTCAGCAACCGCTTCCTGAAGAAGAAGCCGATGGAGTTTGGTTCGTGGACCACACGAGAAATCCTGAGCGCGTCTTTCGCCGGGGTGCGTGGGACGATCACTCTGGCCGGTGTGCTCTCTATCCCGCTGCTCTTGCCGGATGGTAACGGCTTCCCGGCGCGCTATGAGCTGGTGTTCCTGGCGGCTg                                                                                                                                                          <  2:255945/245‑1 (MQ=255)                                                                                       atgagtgtGATATAGGCGTCGGTGTTGATGGTCTGTTTCGGCTGGCTGTGGACGATGAAAAAGTTCAGCAACCGCTTCCTGAAGAAGAAGCCGATGGAGTTTGGTTCGTGGACCACACGAGAAATCCTGATCGCGTCTGTCGCCGGGGTGCGTGGTACGATCACGCTGGCCGGTGTGCTCTCTATCCCGCTGCTCTTGCCGGATGGTAACGTCTGCCCGGAGCGCTATGAGCTGGTGTTCCTGGCGGCTgg                                                                                                                                                         <  2:332362/244‑1 (MQ=255)                                                                                                 tatatGCGGCGCTGATGCTGGTCCGTTTCGGCTGGCTGTGGACGATGAAAAAGTTAAGCAACCGCTTCCTGAAGAAGAAGCCGATGGAGTTTGGTTCGTGGACCACACGAGAAATCATGATCGCGTCTTTCGCCGGGGTGCGTGGGACGATCACTCTGGCAGGTGTGCTCTCTATCCCGCTGCTCTTGCCGGATGGTAACGTCTTCCCGGCGCGCTATGAGCTGGTGTTCCTGGCGGCTGGCGTCAttctc                                                                                                                                               >  1:170831/1‑251 (MQ=255)                                                                                                    atGCGGCGCTGATGCTGGTCCGTTTCGGCTGGCTGTGGACGATGAAAAAGTTCAGCAACCGCTTCCTGAAGAAGAAGCCGATGGAGTTTGGTTCGTGGACCACACGAGAAATCCTGATCGCGTCTTTCGCCGGGGTGCGTGGGACGATCACTCTGGCCGGTGTGCTCTCTATCCCGCTGCTCTTGCCGGATGGTAACGTCTTCCCGGCGCGCTATGAGCTGGTGTTCCTGGCGGCTGGCGTCAttctcttc                                                                                                                                            >  1:414011/1‑251 (MQ=255)                                                                                                           gcTGATGCTGGTCCGTTTCGGCTGGCTGTGGACGATGAAAAAGTTCAGCAACCGCTTCCTGAAGAAGAAGCCGATGGAGTTTGGTTCGTGGACCACACGAGAAATCCTGATCGCGTCTTTCGCCGGGGTGCGTGGGACGATCACTCTGGCCGGTGTGCTCTCTATCCCGCTGCTCTTGCCGGATGGTAACGTCTTCCCGGCGCGCTATGAGCTGGGGTTCCTGGCGGCTGGCGTCATTCTCTTCTCGCTGt                                                                                                                                     >  2:359327/1‑251 (MQ=255)                                                                                                              gATGCTGGTCCGTTTCGGATGGCTGTGGACGATGAAAAAGTTCAGCAACCGCTTCCTGAAGAAGAAGCCGATGGAGTTTGGTTCGTGGACCACACGAGAAATCCTGATCGCGTCTTTCGCCGGGGTGCGTGGGACGATCACTCTGGCCGGTGTGCTCTCTATCCCGCTGCTCTTGCCGGATGGTAACGTCTTACCGGCGCGCTATGAGCTGGTGTTCCTGGCGGCTGGCGTCATTCTCTTCTCGCTGtttt                                                                                                                                  >  1:17934/1‑250 (MQ=255)                                                                                                                 gCTGGTCCGTTTCGGCTGGCTGTGGACGATGAAAAAGTTCAGCAACCGCTTCCTGAAGAAGAAGCCGATGGAGTTTGGTTCGTGGACCACACGAGAAATCCTGATCGCGTCTTTCGCCGGGGTGCGTGGGACGATCACTCTGGCCGGTGTGCTCTCTATCCCGCTGCTCTTGCCGGATGGTAACGTCTTCCCGGCGCGCTATGAGCTGGTGTTCCTGGCGGCTGGCGTCCTTCTCTTCTCGCTGttaggtc                                                                                                                               >  2:471677/1‑246 (MQ=255)                                                                                                                 gCTGGTCCGTTTCGGCTGGCTGTGGACGATGAAAAAGTTCAGCAACCGCTTCCTGAAGAAGAAGCCGATGGAGTTTGGTTCGTGGACCACACGAGAAATCCTGATCGCGTCTTTCGCCGGGGTGCGTGGGACGATCACTCTGGCCGGTGTGCTCTCTATCCCGCTGCTCTTGCCGGATGGTAACGTCTTCCCGGCGCGCTATGAGCTGGTGTTCCTGGCGGCTGGCGTCATTCTCTTCTCGCTGtt                                                                                                                                    <  1:471677/246‑1 (MQ=255)                                                                                                                    ggTCCGTTTCGGCGTGCTGTGGACGATGAAAAAGGTCAGCATCCGCTTCCTGAAGAAGAAGCCGATGGAGTTTGGTTCGTGGACCACACGAGAAATCCTGATCGCGTCTTTCGCCGGGGTGCGTGGGACGATCACTCTGGCCGGTGTGCTCTCTACCCCGCTGCTCTGGCCGGATGGTAACGTCTTCCCTGCGCGCTATGAGCTGGGGTTCCTGGCGGCTGGCGTCATTCTCTTCTCGCTGTTTGTCGGCg                                                                                                                            <  2:470042/251‑1 (MQ=255)                                                                                                                     gTCCGTTTCGGCTGGCTGTGGACGATGAAAAAGTTCAGCAACCGCTTCCTGAAGAAGAAGCCGATGGAGTTTGGTTCGTGGACCACACGAGAAATCCTGATCGCGTCTTTCGCCGGGGTGCGTGGGACGATCACTCTGGCCGGTGTGCTCTCTATCCCGCTGCTCTTGCCGGATGGTAACGTCTTccc                                                                                                                                                                                          >  2:485890/1‑188 (MQ=255)                                                                                                                     gTCCGTTTCGGCTGGCTGTGGACGATGAAAAAGTTCAGCAACCGCTTCCTGAAGAAGAAGCCGATGGAGTTTGGTTCGTGGACCACACGAGAAATCCTGATCGCGTCTTTCGCCGGGGTGCGTGGGACGATCACTCTGGCCGGTGTGCTCTCTATCCCGCTGCTCTTGCCGGATGGTAACGTCTTccc                                                                                                                                                                                          <  1:485890/188‑1 (MQ=255)                                                                                                                       cctgttCGGCGGGCTGTGGACGATGAAAAAGTTAAGCAACCGCTGCCTGAAGAAGAAGCCGATGGAGTTTGGTTCGTGGACCACACGAGAAATCCTGATCGCGTCTTGCGCCGGGGTGCGTGGGACGATCACTCTGGCCGGTGTGCGCTCTATCCCGCTGCTCTTGCCGGATGGTAACGTCTGCCCGGCGCGCTATGAGCTGGTGTTCCTGGCGGCTGGCGTCATTCTCTTCTCGCTGTTTGTCGGCgtg                                                                                                                          <  2:17934/246‑1 (MQ=255)                                                                                                                         gTTTCGGCTGGCTGTGGACGATGAAAAAGTTCAGCAACCGCTTCCTGAAGAAGAAGCCGATGGAGTTTGGTTCGTGGACCACACGAGAAATCCTGATCGCGTCTTTCGCCGGGGTGCGTGGGACGATCACTCTGGCCGGTGTGCTCTCTATCCCGCTGCTCTTGCCGGATGGTaa                                                                                                                                                                                                   >  1:116191/1‑175 (MQ=255)                                                                                                                         gTTTCGGCTGGCTGTGGACGATGAAAAAGTTCAGCAACCGCTTCCTGAAGAAGAAGCCGATGGAGTTTGGTTCGTGGACCACACGAGAAATCCTGATCGCGTCTTTCGCCGGGGTGCGTGGGACGATCACTCTGGCCGGTGTGCTCTCTATCCCGCTGCTCTTGCCGGATGGTaa                                                                                                                                                                                                   <  2:116191/175‑1 (MQ=255)                                                                                                                               gctggctgTGGACGATGAAAAAGTTCAGCAACCGCTTCCTGAAGAAGAAGCCGATGGAGTTTGGTTCGTGGACCACACGAGAAATCCTGATCGCGTCTTTCGCCGGGGTGCGTGGGACGATCACTCTGGCCGGTGTGCTCTCTATCCCGCTGCTCTTGCCGGATGGTAACGTCTTCCCGGCGCGCTATGAGCTGGTGTTCCTGGCGGCTGGCGTCATTCTCTTCTCGCTGTTTGTCGGCGTGGTGATGTTg                                                                                                                 >  1:410795/1‑251 (MQ=255)                                                                                                                                  ggctgTGGACGATGAAAAAGTTCAGCAACCGCTTCCTGAAGAAGAAGCCGATGGAGTTTGGTTCGTGGACCACACGAGAAATCCTGATCGCGTCTTTCGCCGGGGTGCGTGGGACGATCACTCTGGCCGGTGTGCTCTCTATCCCGCTGCTCTTGCCGGATGGTAACGTCTTCCCGGCGCGCTATGAGCTGGTGTTCCTGGCGGCTGGCGTCATTCTCTTCTCGCTGTTTGTCGGCGTGGTGATGTTGCCt                                                                                                              >  1:265571/1‑251 (MQ=255)                                                                                                                                      gtgGTCGATGAAAAAGTTCAGCAACCGCTTCCTGAAGAAGAAGCCGATGGAGTTTGGTTCGTGGACCACACGAGAAATCCTGATCGCGTCTTTCGCCGGGGTGCGTGGGACGATCACTCTGGCCGGTGTGCTCTCTATCCCGCTGCTCTTGCCGGATGGTAACGTCTTCCCGGCGCGCTATGAGCTGGTGTTCCTGGCGGCTGGCGTCATTCTCTTCTCGCTGTTTGTCGGCGTGGTGATGTTGCCTATTc                                                                                                          <  2:318058/251‑1 (MQ=255)                                                                                                                                      gtgGACGATGAAAAAGTTCAGCACCCGCTTCCTGAAGAAGAATCCGATGGAGTTTGGTTCGCTGACCACACGAGAAATCCTGAGCGTGTCTTTCGCCGTGGTGCGTGGGACGATCGCTCTGGCCGGTGTGCTCTCTATCCCGCTGCTCTTGCCGGATGGTAACGTCTTCCCGGCGCGCTATGAGCTGGTGTTCCTGGCGGCTGGCGTCATTCTCTTCTCGCTGCTTGTCGGCGGGGTGATGTTGCCTAtt                                                                                                           <  2:268810/250‑1 (MQ=255)                                                                                                                                      gtgGACGATGAAAAAGTTCAGCAACCGCTTCCTGAAGAAGAAGCCGATGGAGTTTGGTTCGTGGACCACACGAGAAATCCTGATCGCGTCTTTCGCCGGGGTGCGTGGGACGATCACTCTGGCCGGTGTGCTCTCTATCCCGCTGCTCTTGCCGGATGGTAACGTCTTCCCGGCGCGCTATGAGCTGGTGTTCCTGGCGGCTGGCGTCATTCTCTTCTCGCTGTTTGTCGGCGTGGTGATGTTGCCTAt                                                                                                            <  1:360941/249‑1 (MQ=255)                                                                                                                                        ggACGTTGAAAAAGTTCAGCAACCGCTTCCTGAAGAAGAAGCCGATGGAGTTTGGTTCGTGGACCACACGAGAAATCCTGATCGCGTCTTTCGCCGGGGTGCGTGGGACGATCACTCTGGCCGGTGTGCTCTCTATCCCGCTGCTCTTGCCGGATGGTAACGTCTTCCCGGCGCGCTATGAGCTGGTGTTCCTGGCGGCTGGCGTCATTCTCTTCTCGCTGTTTGTCGGCGTGGTGATGTTGCCTATTCt                                                                                                         <  2:398301/250‑1 (MQ=255)                                                                                                                                               aaaaaaGTTCAGCAACCGCTTCCTGAAGAAGAAGCCGATGGAGTTTGGTTCGTGGACCACACGAGAAATCCTGATCGCGTCTTTCGCCGGGGTGCGTGGGACGATCACTCTGGCCGGTGTGCTCTCTATCCCGCTGCTCTTGCCGGATGGTAACGTCTTCCCGGCGCGCTATGAGCTGGTGTTCCTGGCGGCTGGCGTCATTCTCTTCTCGCTGTTTGTCGGCGTGGTGATGTTGCCTATTCTGCTacaac                                                                                                 <  2:277968/250‑1 (MQ=255)                                                                                                                                                 aaaaGTTCAGCAACCGCTTCCTGAAGATGAAGCCGATGGAGTTTGGTTCGTGGACCACCCGAGAAATCCTGATCGCGTCTTTCGCCGGGGTTCGTGGGACGATCACTCTGGCCGGTGTGCTCTCTATCCCGCTGCTCTTGCCGGATGGTAACGTCTTCCCGGCGCGCTATGAGCTGGTGTTCCTGGCGGCTGGCGTCATTCTCTTCTCGCTGTTTGTCGGCGTGGTGATGTTGCCTATTCTGCTACAacac                                                                                               <  2:14686/251‑1 (MQ=255)                                                                                                                                                   aaGTTCAGCAACCGCGTCCTGAAGAAGAAGCCGATGGAGTTGTGTTCGTGGACCACACGAGAAATCCTGATCGCGTCTTTCGCCGGGGTGCGTGGGACGATCACTCTGGCCGGTGTGCTCTCTATCCCGCTGCTCTTGCCGGATGGTAACGTCTTCCCGGCGCGCTATGAGCTGGTGTTCCTGGCGGCTGGCGTCATTCTCTTCTCGCTGTTTGTCGGCGTGGTGATGTTGCCTATTCTGCTACAACACAt                                                                                             <  2:192008/251‑1 (MQ=255)                                                                                                                                                             aCCGCTTCCTGAAGAAGCAGCCGATGGAGTTTGGTTCGTGGACCACACGAGAAATCCTGATCGCGTCTTTCGCCGGGGTGCGTGGGACGATCACTCTGGCCGGTGTGCTCTCTATCCCGCTGCTCTTGCCGGATGGTAACGTCTTCCCGGCGCGCTATGAGCTGGTGTTCCTGGCGGCTGGCGTCATTCTCTTCTCGCTGTTTGTCGGCGTGGTGATGTTGCCTATTCTTCTACAACACATTGAAGTcgcg                                                                                   >  1:414665/1‑251 (MQ=255)                                                                                                                                                             aCCGCTTCCTGAAGAAGAAGCCGATGGAGTTTGGTTCGTGGACCACACGAGAAATCCTGATCGCGTCTTTCGCCGGGGTGCGTGGGACGATCTCTCTGGCCGGTGTGCTCTCTATCCCGCTGCTCTTGCCGGATGGTAACGTCTTCCCGGCGCGCTATGAGCTGGTGTTCCTGGCGGCTGGCGTCATTCTCTTCTCGCTGTTTGTCGGCGTGGTGATGTTGCCTATTCTGCTACAACACATTGAAGTcgcg                                                                                   <  2:414011/251‑1 (MQ=255)                                                                                                                                                                  ttCCTGAAGAAGAAGCCGATGGAGTTTGGTTCGTGGACCACACGAGAAATCCTGATCGCGTCTTTCGCCGGGGTGCGTGGGACGATCACTCTGGCCGGTGTGCTCTCTATCCCGCTGCTCTTGCCGGATGGTAACGTCTTCCCGGCGCGCTATGAGCTGGTGTTCCTGGCGGCTGGCGTCAttctctt                                                                                                                                             <  1:164384/188‑1 (MQ=255)                                                                                                                                                                  ttCCTGAAGAAGAAGCCGATGGAGTTTGGTTCGTGGACCACACGAGAAATCCTGATCGCGTCTTTCGCCGGGGTGCGTGGGACGATCACTCTGGCCGGTGTGCTCTCTATCCCGCTGCTCTTGCCGGATGGTAACGTCTTCCCGGCGCGCTATGAGCTGGTGTTCCTGGCGGCTGGCGTCAttctctt                                                                                                                                             >  2:164384/1‑188 (MQ=255)                                                                                                                                                                   gcccgaagaagaaGTCGATGGAGTGTGGTTCGTTGACCACACGAGAGAACCTTATCGCGTCTTGCGCCTGGGTGCGTGGGACGCTCACTTTGGCCGGTGTGCTCTTAATCCCGCTGTTCTTGCCGGCTGGTAACGTCGTCTCGGCGCGCTATGAGCGGGTGTTCCGGGCGGCTGGCGTCATTCTCTTCTCGCTGTTTGTCGGCGTGGTTATGTTGCCTATTCTGTTACAACACATTGAATTCGCGGATc                                                                               <  2:469287/245‑1 (MQ=255)                                                                                                                                                                        aagaagaagCCGATGGAGTTTGGTTCGTGGACCACACGAGAAATCCTGATCGCGTCTTTCGCCGGGGTGCGTGGGACGATCACTCTGGCCGGTGTGCTCTCTATCCCGCTGCTCTTGCCGGATGGTAACGTCTTCCCGGCGCGCTATGAGCTGGTGTTCCTGGCGGCTGGCGTCATTCTCTTCTCGCTGTTTGTCGGCGTGGTGATGTTGCCTATTCTGCTACAACACATTGAAGTCGCGGATCATTCGca                                                                        <  1:390464/251‑1 (MQ=255)                                                                                                                                                                           aagaagCCGATGGAGTTTGGTTCGTGGACCACACGAGAAATCCTGATCGCGTCTTTCGCCGGGTGGCGTGGGACGATCACTCTGGCCGGTGTGCTCTCTATCCCGCTGCTCTTGCCGGATGGTAACGTCTTCCCGGCGCGCTATGAGCTGGTGTTCCTGGCGGCTGGCGTCATTCTCTTCTCGCTGTTTGTCGGCGTGGTGATGTTGCCTATTCTGCTACAACACATTGAAGTCGCGGATCATTCGcaaca                                                                     <  1:180922/251‑1 (MQ=255)                                                                                                                                                                           aagaagCCGATGGAGTTTGGTTCGTGGACCACACGAGAAATCCTGATCGCGTCTTTCGCCGGGGTGCGTGGGACGATCACTCTGGCCGGTGTGCTCTCTATCCCGCTGCTCTTGCCGGATGGTAACGTCTTCCCGGCGCGCTATGAGCTGGTGTTCCTGGCGGCTGGCGTCATTCTCTTCTCGCTGTTTGTCGGCGTGGTGATGTTGCCTATTCTGCTACAACACATTGAAGTCGCGGATCATTCGcaac                                                                      >  2:164696/1‑250 (MQ=255)                                                                                                                                                                             gaagCCGATTGAGTTTGGTTCGTGGACGACACGAGAAATCCTGATCGCGTCTTTCGCCGGGGTGCGTGGGACGATCACTCTGGCCGGTGTGCTCTCTATCCCGCTGCTCTTGCCGGATGGTAACGTCTTCCCGGCGCGCTATGAGCTGGTGTTCCTGGCGGCTGGCGTCATTCTCTTCTCGCTGTTTGTCGGCGTGGTGATGTTGCCTATTCTGCTACAACACATTGAAGTCGCGGATCATTCGCAACAAt                                                                   <  2:330240/251‑1 (MQ=255)                                                                                                                                                                                         gTTTGGTTCGTGGACCACACGAGAAATACTGATCGCGTCTTTCACCGGGGTGCGTGGGACGATCACTCTGGCCGGCGTGCTCTCTATCCCGCTGCTCTTGCCGGATGGTAACGTCTTCCCGGCGCGCTATGCGCTGGTGTTCCTGGCGGCTGGCGTCATTCTCTTCTCGCTGTTTGTCGGCGTGGTGATGTTGCCTATTCTGCTACAACCCATTGAAGTCGCGGATCCTTCCCACCAATTGAACGAGGAAc                                                       >  2:63659/1‑251 (MQ=255)                                                                                                                                                                                             ggTTCGTGGACCACACGAGAAATCCTGATCGCGTCTTTCGCCGGGGTGCGTGGGACGATCACTCTGGCCGGTGTGCTCTCTATCCCGCTGCTCTTGCCGGATGGTAACGTCTTCCCGGCGCGCTATGAGCTGGTGTTCCTGGCGGCTGGCGTCATTCGCTTCTCGCTGTTTGTCGGCGTGGTGATGTTGCCTATTCTGCTACAACACATTGAAGTCGCGGATCATTCGCAACAATTGAAAGAGGAACGTAt                                                   <  1:203914/251‑1 (MQ=255)                                                                                                                                                                                                 cGTGGACCACACGAGAAATCCTGATCGCGTCTTTCGCCGGGGTGCGTGGGACGATCACTCTGGCCGGTGTGCTCTCTATCCCGCTGCTCTTGCCGGATGGTAACGTCTTCCCGGCGCGCTATGAGCTGGTGTTCCTGGCGGCTGGCGTCATTCTCTTCTCGCTGTTTGTCGGCGTGGTGATGTTGCCTATTCTGCTACAACACATTGAAGTCGCGGATCATTCGCAACAATTGAAAGAGGAACGTATTgc                                                <  1:110272/250‑1 (MQ=255)                                                                                                                                                                                                       ccACACGAGAAATCCTGATCGCGTCTTTCGCCGGGGTGCGTGGGACGATCACTCTGGCCGGTGTGCTCTCTATCCCGCTGCTCTTGCCGGATGGTAACGTCTTCCCGGCGCGCTATGAGCTGGTGTTCCTGGCGGCTGGCGTCATTCTCTTCTCGCTGTTTGTCGGCGTGGTGATGTTGCCTATTCTGCTACAACACATTGAAGTCGCGGATCATTCGCAACATTTGAAAGAGGAACGTATTGcgcgcgcg                                         >  2:323598/1‑251 (MQ=255)                                                                                                                                                                                                        cacaCGAGAAATCCTGATCGCGTCTTTCGCCGGGGTGCGTGGGACGATCACTCTGGCCGGTGTGCTCTCTATCCCGCTGCTCTTGCCGGATGGTAACGTCTTCCCGGCGCGCTATGAGCTGGTGTTCCTGGCGGCTGGCGTCATTCTCTTCTCGCTGTTTGTCGGCGTGGTGATGTTGCCTATTCTGCTACAACACATTGAAGTCGCGGATCATTCGCAACAATTGAAAGAGGAACGTATTGCGCGAGCgg                                        <  1:149968/251‑1 (MQ=255)                                                                                                                                                                                                             gtgaAATCCGGTTCGCGTCCTTCGCCGGGGGGCGGGGGACGATACCTCTGGCCGGTGGGCTCTCTATCCCGCTGCTCTTGCCGGATGGTAACGTCTTCCCGGCGCGCGATGAGCTGGTGTTCCTGGCGGCTGGCGTCATGCTCTTCGCGCTGTTTGTCGGCGTGGTGATGTGGCCTATTCTGCTACAACACATTGAAGTCGCGGATCATTCGCAACAATTGAAAGATGAACGTATTGCGCGAGCGGCAACg                                   <  2:252347/249‑1 (MQ=255)                                                                                                                                                                                                                aaaTCCTGATCGCGTCTTTCGCCGGGGTGCGTGGGACGATCACTCTGGCCGGTGTGCTCTCTATCCCGCTGCTCTTGCCGGATGGTAACGTCTTCCCGGCGCGCTATGAGCTGGTGTTCCTGGCGGCTGGCGTCATTCTCTTCTCGCTGTTTGTCGGCGTGGTGATGTTGCCTATTCTGCTACAACACATTGAAGTCGCGGATCATTCGCAACAATTGAAAGAGGAACGTATTGCGCGAGCGGCAACGGc                                 >  1:77761/1‑250 (MQ=255)                                                                                                                                                                                                                 aaTCCTGATCGCGTCTTTCGCCGGGGTGCGTGGGACGATCACTCTGGCCGGTGTGCTCTCTATCCCGCTGCTCTTGCCGGATGGTAACGTCTTCCCGGCGCGCTATGAGCTGGTGTTCCTGGCGGCTGGCGTCATTCTCTTCTCGCTGTTTGTCGGCGTGGTGATGTTGCCTATTCTGCTACAACACATTGAAGTCGCGGATCATTCGCAACAATTGAAAGAGGAACGTATTGCGCGAGCGGCAACGGCAg                               <  1:164321/251‑1 (MQ=255)                                                                                                                                                                                                                  aTCCTGGTCGCGTCTTTCGCCGGGGTGCGTGGGACGATCACTCTGGCCGGTGTGCTCTCTAGCCCGCTGCTCTTGCCGGATGGTAACGTCTTCCCGGCGCGCTATGAGCTGGTGTTCCTGGCGGCTGGCGTCATTCTCTTCTCGCTGTTTGTCGGCGTGGTGATGTTGCCTATTCTGCTACAACACATTGAAGTCGCGGATCATTCGCAACAATTGAAAGAGGAACGTATTGCGCGAGCGGCAAc                                    <  2:172360/245‑1 (MQ=255)                                                                                                                                                                                                                  aTCCTGATCGCGTCTTTCGCCGGGGTGCGTGGGACGATCACTCTGGCCGGTGTGCTCTCTATCCCGCTGCTCTTGCCGGATGGTAACGTCTTCCCGGCGCGCTATGAGCTGGTGTTCCTGGCGGCTGGCGTCATTCTCTTCTCGCTGTTTGTCGGCGTGGTGATGTTGCCTATTCTGCTACAACACATTGAAGTCGCGGATCATTCGCAACAATTGAAAGAGGAACGTATTGCGCGAGCGGCAAc                                    >  1:172360/1‑245 (MQ=255)                                                                                                                                                                                                                    ccTGATCGCGTCTTTCGCCGGGGTGCGTGGGACGATCACTCTGGCCGGTGTGCTCTCTATCCCGCTGCTCTTGCCGGATGGTAACGTCTTCCCGGCGCGCTATGAGCTGGTGTTCCTGGCGGCTGGCGTCATTCTCTTCTCGCTGTTTGTCGGCGTGGTGGTGTTGCCTATTCTGCTACAACACATTGACGTCGCGGATCATTCGCAACAATTGAAAGTGGAACGTATTGCGCGAGCGGCCACGGCAGa                              >  2:118138/1‑249 (MQ=255)                                                                                                                                                                                                                                    gCCGGGGGGCGTGGGACGATCACTCTGGCCGGGGTGCTCTCTATCCCGCTGCTCTTGCCGGATGGTAACGTCTTCCCGGCGCGCTATGAGCTGGTGTTCCTGGCGGCTGGCGTCATTCTCTTCTCGCTGTTTGTCGGCGTGGTGATGTTGCCTATTCTGCTACAACACATTGAAGTCGCGGATCATTCGCAACAATTGAAAGAGGAACGTATTGCGCGAGCGGCAACGGCAGAAGTGGCGATTGTGGCGAt            <  2:265571/251‑1 (MQ=255)                                                                                                                                                                                                                                              gTGGGACGATCACTCTGGCCGGTGTGCTCTCTATCCCGCTGCTCTTGCCGGATGGTAACGTCTTCCCGGCGCGCTATGAGCTGGTGTTCCTGGCGGCTGGCGTCATTCTCTTCTCGCTGTTTGTCGGCGTGGTGATGTTGCCTATTCTGCTACAACACATTGAAGTCGCGGATCATTCGCAACAATTGAAAGAGGAACGTATTGCGCGAGCGGCAACGGCAGAAGTGGCGATTGTGGCGATCCAGAAAATg  <  2:410795/251‑1 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                      GTCTGCAGCTGCCGGGTATTCTGGAGACGTCGCTGATGGCAGCAGAAATCGACCCTAACGTCGAAATCTGGATGCTGTTTACCAATATTATTCTGATATATGCGGCGCTGATGCTGGTCCGTTTCGGCTGGCTGTGGACGATGAAAAAGTTCAGCAACCGCTTCCTGAAGAAGAAGCCGATGGAGTTTGGTTCGTGGACCACACGAGAAATCCTGATCGCGTCTTTCGCCGGGGTGCGTGGGGCGATCACTCTGGCCGGTGTGCTCTCTATCCCGCTGCTCTTGCCGGATGGTAACGTCTTCCCGGCGCGCTATGAGCTGGTGTTCCTGGCGGCTGGCGTCATTCTCTTCTCGCTGTTTGTCGGCGTGGTGATGTTGCCTATTCTGCTACAACACATTGAAGTCGCGGATCATTCGCAACAATTGAAAGAGGAACGTATTGCGCGAGCGGCAACGGCAGAAGTGGCGATTGTGGCGATCCAGAAAATG  >  NC_000913/4280851‑4281338

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑