Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,223,638	T→C	intergenic (‑10/‑190)	iclR ← / → metH	DNA‑binding transcriptional repressor IclR/cobalamin‑dependent methionine synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,223,638	0	T	C	100.0%	137.4 / NA	46	intergenic (‑10/‑190)	iclR/metH	DNA‑binding transcriptional repressor IclR/cobalamin‑dependent methionine synthase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (9/37);  total (9/37)

AGTTCGCCAACCTGACGCACGAAACCCTGCTGTTGCATCGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGA‑CCATGACAGTCTCTTTTTTCTGTATCG‑TGGAAATCATTTTCATTTTTATT‑GTTAGCTAATGCAATAGTTACTGAACTGATCCGATGA‑GTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCT  >  NC_000913/4223393‑4223883                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                         aGTTCGCCAACCTGACGCACGAAACCCTGCTGTTGCATCGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGA‑CCATGACAGTCTCcttttt                                                                                                                                                                                                                                                     <  1:181778/251‑1 (MQ=255) aGTTCGCCAACCTGACGCACGAAACCCTGCTGTTGCATCGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGA‑CCATGACAGTCTCcttttt                                                                                                                                                                                                                                                     <  1:59166/251‑1 (MQ=255)  gTTCGCCAACCTGACGCACGAAACCCTGCTGTTGCATCGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGA‑CCATGACAGTCTCCTTTTTc                                                                                                                                                                                                                                                    <  1:206644/251‑1 (MQ=255)       ccAACCGGACGCACGAAACCCTGCTGTTGCTTCGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGA‑CCATGACAGTCTCCTTTTTCTGTAt                                                                                                                                                                                                                                               <  2:424725/251‑1 (MQ=255)        cAACCTGACGCACGGAACCCTGTTTGTGCATCGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCTGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGA‑CCATGACAGTCTCCTTTTTCTGTATc                                                                                                                                                                                                                                              <  2:400417/251‑1 (MQ=255)        cAACCTGACGCACGAAACCCTGCTGTTGCATCGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCCCACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGA‑CCATGACAGTCTCCTTTTTCTGTATc                                                                                                                                                                                                                                              <  2:213361/251‑1 (MQ=255)              gACGCACGAAACCCTGCTGTTGCATCGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGA‑CCATGACAGTCTCCTTTTTCTGTATCG‑Tgg                                                                                                                                                                                                                                         <  2:342891/249‑1 (MQ=255)              gACGCACGAAACCCTGCTGTTGCATCGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGA‑CCATGACAGTCTCCTTTTTCTGTATCG‑TGGaa                                                                                                                                                                                                                                       <  1:295663/251‑1 (MQ=255)               aCGCACGAAACCCTGCTGTTGCAGCGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGA‑CCATGACAGTCTCCTTTTTCTGTATCG‑TGGaaa                                                                                                                                                                                                                                      <  1:476831/251‑1 (MQ=255)                cGCACGAAACCCTGCTGTTGCATCGTGGTTAACAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGGTCCGTGTGTGTCACGCTGCCATTGGATTCGGCAATCCACTCCAGTAAGTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCGGGTGCGGGGGCAACGACGGGTTTTCTTCCGCGTTGCGCGGGAATTGGTGCGA‑TCATGACAGTCTCCTTGTTCTGTATCG‑TTGAAAt                                                                                                                                                                                                                                     <  2:447653/251‑1 (MQ=255)                         cccTTCTGTTTCATCGTGGTGAGAATGCGGTGGGTTGTGGAATGGGGGTACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGGTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGTCAACGGCGGGTTTTCTTCCGCGTTTCGCGGGAATGGGTGCGA‑CCATGACAGTCTCCTTTTTCTGTATCG‑TGGAAAtcattttcat                                                                                                                                                                                                                            <  2:239122/251‑1 (MQ=255)                           ctgcttTTGCATCGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGA‑CCATGACAGTCTCCTTTTTCt                                                                                                                                                                                                                                                   <  2:464663/227‑1 (MQ=255)                                               gCAGGCGGGTGGTCGTGTAAGTGGTTAACCCGGTTTGTTGCGCTAGTTTCTTGATTTCCATACGGCCATTGGATTCGGCAATCCCCTCCAGTAATTTCAGTCCACGCGTTAAAGACTGAACCTGTTCAGTCGCTGGGGCGGTGGCAACGGCGGGTTTTCTGCCGCGTATCGCGGGAATGGGTGCGA‑CCATTACAGTCTCCTATTGCTGTATCG‑TGGAAAGCATTTTCATTTTTATT‑GTTAGCTAATGctat                                                                                                                                                                                                     <  2:375635/251‑4 (MQ=255)                                                    cGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGA‑CCATGACAGTCTCCTTTTTCTGTATCG‑TGGAAAtcattttc                                                                                                                                                                                                                              <  1:112935/222‑1 (MQ=255)                                                    cGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGA‑CCATGACAGTCTCCTTTTTCTGTATCG‑TGGAAATCATTTTCATTTTTATT‑GTTAGCTAATGCAATAGTTa                                                                                                                                                                                                <  1:127883/251‑1 (MQ=255)                                                        gggTCGTGGAATTGGGAAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGTGTTTCGCGGGAATGGGTGCGA‑TCATGACAGTCTCCTTTTTCTGTATCG‑TGGAAATCATTTTCATTTTTATT‑GTTAGCTAATGCAATAGTTactga                                                                                                                                                                                            <  2:39518/251‑1 (MQ=255)                                                                               gCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGGTTTTTGGCGCGTTTCGCGGGAAAGGGTGCGC‑CCCTGTAAGGCTCCTTTTTCTGTATCGGTGGAA‑TTATTTTTATTTTTTTT‑TTTTGCTAATGCACTAGTTTCTGAACTTGTCCGATTA‑GTTATTGttt                                                                                                                                                                    >  1:333662/1‑250 (MQ=255)                                                                                cctgttggcccAGTTCCGTGAGTGCCACACTGCCAATGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGGTAAAGACTGAACCTGTCCAGTCGCTGGGGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGA‑CCATGACAGTCTCCTTTTTCTGTATCG‑TGGAAATCATTTTCATGTTTATT‑GTTAGCTAATGCAATAGTTACTGAACTGATCCGATGA‑GGTAATGTTGa                                                                                                                                                                   <  2:69341/242‑1 (MQ=255)                                                                                  tgttgCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGA‑CCATGACAGTCTCCTTTTTCTGTATCG‑TGGAAATCATTTTCATTTTTATT‑GTTAGCTAATGCAATAGTTACTGAACTGATCCGATGA‑GTTAATGTTGaa                                                                                                                                                                  <  1:428800/250‑1 (MQ=255)                                                                                          cAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGA‑CCATGACAGTCTCCTTTTTCTGTATCG‑TGGAAATCATTTTCATTTTTATT‑GTTAGCTAATGCAATAGTTACTGAACTGATCCGATGA‑GTTAATGTTGAACAAATCTCa                                                                                                                                                         <  2:193002/251‑1 (MQ=255)                                                                                               ccGTTAGTGCCACACTGCCATTGGATTCGTCAATCCACGCCAGTAATTGCATGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGTTGCGA‑CCATGACAGTCTCCTTTTTCTGTATCG‑TGGAAATCATTTTCATTTTTATT‑GTTAGCTAATGCAATAGTTACTGAACTGATCCGATGA‑GTTAATGTTGAACAAATCTCATGtt                                                                                                                                                     <  2:432339/250‑1 (MQ=255)                                                                                                            acTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGA‑CCATGACAGTCTCCTTTTTCTGTATCG‑TGGAAATCATTTTCATTTTTATT‑GTTAGCTAATGCAATAGTTACTGAACTGATCCGATGA‑GTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCt                                                                                                                                       <  1:352906/251‑1 (MQ=255)                                                                                                                    tGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGGTTTCTTCCGCGTTTTGCGGGGAAGGGGGCGAACCA‑GTCACTCTCCTTTTTCTGTATCG‑TGGGAATCATTTTCATTTTTATT‑TTTACCTCATGCCATAGTTTATGAAATGTTCCACTGA‑GTTTATTTTGAACAAATCTCCTGTTGCGTGGGGGGCGCGTTTTCcac                                                                                                                               >  1:97155/1‑251 (MQ=255)                                                                                                                                tCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGGTTTTTGCCGCGTTTTCCGGGGATTGGGGCGA‑CCCTTAAAGTCCCCTTTTTTTGTTTCG‑TGGGAATCACTTTCCTTTTTATTTATTA‑CTAATGGAATAGTTTCTGTACTGATCCGATGAAGTTA‑TTTTTAACAAATCTCATTTTGCGGGGTGGTCGCtttttacccagactcgtttat                                                                                                                   >  1:257426/1‑234 (MQ=255)                                                                                                                                  cACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGA‑CCATGACAGTCTCCTTTTTCTGTATCG‑TGGAAATCATTTTCATTTTTATT‑GTTAGCTAATGCAATAGTTACTGAACTGATCCGATGA‑GTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGc                                                                                                                 <  1:155366/251‑1 (MQ=255)                                                                                                                                               tCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGA‑CCATGACAGTCTCCTTTTTCTGTATCG‑TGGAAATCATTTTCATTTTTATT‑GTTAGCTAATGCAATAGTTACTGAACTGATCCGATGA‑GTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTAt                                                                                                           <  1:234098/244‑1 (MQ=255)                                                                                                                                                    ccccgcgTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCTCGTGGCGCGGGAATGTGTGCGA‑CCATGACAGTCTCCTTTTTCTGTATCG‑TGGAAATCATTTTCATTTTTATT‑GTTAGCTAATGCAATAGTTACTGAACGGATCCGATGA‑GTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAAt                                                                                               <  2:409884/248‑1 (MQ=255)                                                                                                                                                            tAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGA‑CCATGACAGTCTCCTTTTTCTGTATCG‑TGGAAATCATTTTCATTTTTATT‑GTTAGCTAATGCAATAGTTACTGAACTGATCCGATGA‑GTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTttatt                                                                                        <  2:357842/250‑1 (MQ=255)                                                                                                                                                              aaGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGA‑CCATGACAGTCTCCTTTTTCTGTATCG‑TGGAAATCATTTTCATTTTTATT‑GTTAGCTAATGCAATAGTTACTGAACTGATCCGATGA‑GTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGGTGAATTTTTATTaaa                                                                                     <  1:367347/251‑1 (MQ=255)                                                                                                                                                                          gTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGA‑CCATGACAGTCTCCTTTTTCTGTATCG‑TGGAAATCATTTTCATTTTTATT‑GTTAGCTAATGCAATAGTTACTGAACTGATCCGATGA‑GTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGc                                                                         <  1:219665/251‑1 (MQ=255)                                                                                                                                                                          gTCCAGTCGCTGGGGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGA‑CCATGACAGTCTCCTTTTTCTGTATCG‑TGGAAATCATTTTCATTTTTATT‑GTTAGCTAATGCAATAGTTACTGAACTGATCCGATGA‑GTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGc                                                                         <  2:292104/251‑1 (MQ=255)                                                                                                                                                                           tCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTTTTCCGCGTTTCGCGGGGATGGGGGCGG‑CCATGACAGTCTCCTTTTTTTGTATCG‑GGGGAATCATTTTTATTTTTATT‑TTTAGCTAATGCAATAGTTAATGAAATGATCCGATGA‑GTTAATGTTGAACAAAACTCATGTTGCGTGGTGGTCGCTTTTTCCACAGATGCGTTTTTGCCCGTATGGTTTGTTGGATTTTTTTTAAATCTggggtgtgcg                                                                        >  1:49483/1‑244 (MQ=255)                                                                                                                                                                                 cGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGA‑CCATGACAGTCTCCTTTTTCTGTATCG‑TGGAAATCATTTTCATTTTTATT‑GTTAGCTAATGCAATAGTTACTGAACTGATCCGATGA‑GTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCg                                                                   <  1:475437/250‑1 (MQ=255)                                                                                                                                                                                     ggTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGA‑CCATGACAGTCTCCTTTTTCTGTATCG‑TGGAAATCATTTTCATTTTTATT‑GTTAGCTAATGCAATAGTTACTGAACTGATCCGATGA‑GTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGc                                                              <  2:101416/251‑1 (MQ=255)                                                                                                                                                                                              gCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGA‑CCATGACAGTCTCCTTTTTCTGTATCG‑TGGAAATCATTTTCATTTGTATT‑GTTAGCTAATGCAATAGTTACTGAACTGATCCGATGA‑GTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGag                                                     <  1:368200/251‑1 (MQ=255)                                                                                                                                                                                                 aCGGCGGGTTTGCTGCCGCGTCTCGCGGGAATGGGTGCGT‑CCAGGACAGTCTCCTTTTTCTGTATCG‑TGGAAATCATTTTCATTTTTATT‑GTTAGCTAATGCAATAGTTACTGAACTGATCCGTTGA‑GTTAAGGTTGAACAAAGCTCATGTTGCGTGGTGGTCGCTTTTCCCCCAGATGCGTTTATGCCAGTATTGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGagca                                                   <  1:453028/250‑1 (MQ=255)                                                                                                                                                                                                    gcggGATTTCTGCCGCGTTTCGCGGGAATGGGTGCGA‑CCATGACAGTCTCCTTTTTCTGTATCG‑TGGAAATCATTTTCATTTTTATT‑GTTAGCTAATGCAATAGTTACTGCACTGATCCGATGA‑GTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTCCCACAGATGCGTTTATGCCAGTATGGTTTGTTGCATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAgcagca                                                >  1:432694/1‑250 (MQ=255)                                                                                                                                                                                                        gTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGA‑CCATGACAGTCTCCTTTTTCTGTATCG‑TGGAAATCATTTTCATTTTTATT‑GTTAGCTAATGCAATAGTTACTGAACTGATCCGATGA‑GTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTg                                           <  1:244864/251‑1 (MQ=255)                                                                                                                                                                                                        gTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGA‑CCATGACAGTCTCCTTTTTCTGTATCG‑TGGAAATCATTTTCATTTTTATT‑GTTAGCTAATGCAATAGTTACTGAACTGATCCGATGA‑GTTAATGTTAAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTg                                           >  1:71534/1‑251 (MQ=255)                                                                                                                                                                                                                ccGCGTTTCGCGGGAATGGGTGCGA‑CCATGACAGTCTCCTTTTTCTGTATCG‑TGGAAATCATTTTCATTTTTATT‑GTTAGCTAATGCAATAGTTACTGAACTGATCCg                                                                                                                                                                                   <  2:169921/108‑1 (MQ=255)                                                                                                                                                                                                                ccGCGTTTCGCGGGAATGGGTGCGA‑CCATGACAGTCTCCTTTTTCTGTATCG‑TGGAAATCATTTTCATTTTTATT‑GTTAGCTAATGCAATAGTTACTGAACTGATCCg                                                                                                                                                                                   >  1:169921/1‑108 (MQ=255)                                                                                                                                                                                                                ccGCGTTTCGCGGGAATGGGTGCGA‑CCATGACAGTCTCCTTTTTCTGTATCG‑TGGAAATCATTTTCATGTTTATT‑GTTAGCTAATGCAATAGTTACTGAACTGATCCGATGA‑GTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCGGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACt                                   <  2:56509/251‑1 (MQ=255)                                                                                                                                                                                                                           aggAATGGGTGCGA‑CCAAGTCAGTCTCCTTTTTCTTTATCG‑CGGAAATCATTTTCATTTTTATT‑GTTTGCTCATGCAATAGTTACTGAACTGTTCCGCTGA‑GTTAATGTTGAACAAATCTCATGTTGAGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTCAATCTGGGTTGAGCGTGTCGGGAGCAGCTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGt                        >  2:21449/2‑251 (MQ=255)                                                                                                                                                                                                                            ggAATGGGTGCGA‑CCATGACAGTCTCCTTTTTCTGTATCG‑TGGAAATCATTTTCATTTTTATT‑GTTAGCTAATGCAATAGTTACTGAACTGATCCGATGA‑GTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGtt                       <  1:223522/251‑1 (MQ=255)                                                                                                                                                                                                                              aaTGGGTGCGA‑CCATGACAGTCTCCTTTTTCTGTATCG‑TGGAAATCATTTTCATTTTTATT‑GTTAGCTAATGCAATAGTTACTGAACTGATCCGATGA‑GTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTaa                     <  1:434369/251‑1 (MQ=255)                                                                                                                                                                                                                                             tGACAGTCTCCTTTTTCTGTCTCG‑TGGAAATCATTTTCATTTTTATT‑GTTAGCTAATGCAATAGTTACTGAACTGATCCGATGA‑GTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGAATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGCGCAAGTGTGAGCAGCAAAGGGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTg       >  2:18376/1‑251 (MQ=255)                                                                                                                                                                                                                                              gACAGTCTGCTTTTTCTGTATCG‑TGGAAATCATTGTCATTTTTATT‑GTTAGCTAATGCAATAGTTACTGAACTGATCCGATGA‑GTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTg       <  2:88314/250‑1 (MQ=255)                                                                                                                                                                                                                                                  gTCTCCTTTTTCTGTATCG‑TGGAAATCATTTTCATTTTTAGT‑GTTAGCGAATGCAATAGTTACTGAACTGATCCGATGA‑GTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCGCAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCt  <  1:213273/251‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                         AGTTCGCCAACCTGACGCACGAAACCCTGCTGTTGCATCGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGA‑CCATGACAGTCTCTTTTTTCTGTATCG‑TGGAAATCATTTTCATTTTTATT‑GTTAGCTAATGCAATAGTTACTGAACTGATCCGATGA‑GTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCT  >  NC_000913/4223393‑4223883

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑