Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,215,347 T→A intergenic (+138/‑131) metA → / → aceB homoserine O‑succinyltransferase/malate synthase A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,215,3470TA95.0% 60.6 / ‑3.3 20intergenic (+138/‑131)metA/aceBhomoserine O‑succinyltransferase/malate synthase A
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base A (6/13);  total (7/13)
Fisher's exact test for biased strand distribution p-value = 3.50e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.29e-01

AGCTGGCGTAGTCACGGTAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACGATCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGGGTGCCTTTTTTATTTCCGAAACGTACCTCAGCAGGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTTTAATTAAAATGGAAATTGTTTTTGATTTTGCATTTTAAATGAGTAGTCTTAGTTGTGCTGAACGAAAAGAGCACAACGATCCTTCGTTCACAGTGGGGAAGTTTTCGGATCCATGACGAGGAGCTGCACGATGACTGAACAGGCAACAACAACCGATGAACTGGCTTTCACAAGGCCGTATGGCGAGCAGGAGAAGCAAATTCTTACTGCCGAAGCGGTAGAAT  >  NC_000913/4215105‑4215571
                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                
ggcTGGCGTAGTCACGGTAATTTACTGTT‑GCCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACGATCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGGGTGCCTTTTTTATTTCCGAAACGTACCTCAGCAGGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTATAATTaaaa                                                                                                                                                                                                                         <  2:456790/250‑1 (MQ=255)
             aCGGTAATTGACTGTTGATCAACTGGCTCAACCAGTACGTTTAGTAGAACACTCCATACGATCTAAGGCACAGGAATCCAGCGCGGGATTAATCTTCTGTGTTATTCTATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGTGTGCCTTTTTTATTTCCGAAACGTAACTCAGCAGGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTATAATTAAAATGGAAATTGttt                                                                                                                                                                                                             <  2:330123/251‑1 (MQ=255)
                gTAATTTTCTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACGATCGACGGCACATGAATCCAACGCTGGATTAAACTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGGGTGCCTTTTTTATTTCCGAAACGTACCTCAGCAGGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTATAATTAAAATGGAAATTGTTTTTg                                                                                                                                                                                                          <  2:6226/251‑1 (MQ=255)
                         tGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACGATCTACGGCACATGAATCAAACGCTGGATTAATCTTCTGTGCTCGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGGGTGCCTTTTTTATTTCCGAAACGTACCTCAGAAGGTGAATAAATTTTATTCATCTTGTTATCAACAAGTTATCAAATATTTATAATTAAAATGGAAATTGTTTTTGAATTTGcct                                                                                                                                                                                                 >  1:282266/1‑249 (MQ=255)
                            ttACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACGATCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGGGTGCCTTTTTTATTTCCGAAACGTACCTCAGCAGGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTATAATTAAAATGGAAATTGTTTTTGTTTTTGCAtttt                                                                                                                                                                                              >  1:183252/1‑251 (MQ=255)
                                    tGGCTCAACTATTACGTCTACCAGATCACGCCATACGATCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGGGTGCCTTTTTTATTTCCGAAACGTACCTCAGCAGGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTATAATTAAAATGGAAATTGTTTTTGATTTTGCATTTTAAATGagt                                                                                                                                                                                      >  1:99217/1‑251 (MQ=255)
                                     ggCTCAACTATTACGTCTACCAGATCACGCCATACGATCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGGGTGCCTTTTTTATTTCCGAAACGTACCTCAGCAGGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTATAATTAAAATGGAAATTGTTTTTGATTTTGCATTTTAAATGagt                                                                                                                                                                                      <  1:376718/250‑1 (MQ=255)
                                                                      aCGATCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGGGTGCCTTTTTTATTTCCGAAACGTACCTCAGCAGGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTATAATTAAAATGGAAATTGTTTTTGATTTTGCATTTTAAATGAGTAGTCTTAGTTGTGCTGAACGAAAAGAGCACAACg                                                                                                                                                    <  2:96788/251‑1 (MQ=255)
                                                                                                       aaTCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGGGTGCCTTTTTTATTTCCGAAACGTACCTCAGCAGGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTATAATTAAAATGGAAATTGTTTTTGATTTTGCATTTTAAATGAGTAGTCTTAGTTGTGCTGAACGAAAAGAGCACAACGATCCTGCGTTCACAGTGGTGAAGTTTTCGGAt                                                                                                                    <  1:383451/250‑1 (MQ=255)
                                                                                                            tctGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGGGTGCCTTTTTTATTTCCGAAACGTACCTCAGCAGGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTATAATTAAAATGGAAATTGTTTTTGATTTTGCATTTTAAATGAGTAGTCTTAGTTGTGCTGAACGAAAAGAGCACAACGATCCTTCGTTCACAGTGGGGAAGTTTTCGGATCCa                                                                                                                 <  2:407475/248‑1 (MQ=255)
                                                                                                                                            ccTTACCTCAGGCTCCTTCGGGTGCCTTTTTTATTTCCGAAACGTACCTCAGCAGGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTATAATTAAAATGGAAATTGTTTTTGATTTTGCATTTTAAATGAGTAGTCTTAGTTGTGCTGAACGAAAAGAGCACAACGATCCTTCGTTCACAGTGGGGAAGTTTTCGGATCCATGACGAGGAGCTGCACGATGACTGAACAGGcaaca                                                                              <  2:379407/251‑1 (MQ=255)
                                                                                                                                                      ggCACCTTCGGGTGCCTTTTTTAATTCCGAAACGTACCTCAGCAGGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTATAATTAAAATGGAAATTGTTTTTGATTTTGCATTTTAAATGAGTAGTCTTAGTTGTGCTGAACGAAAAGAGCACAACGATCCTTCGTTCACAGTGGGGAAGTTTTCGGATCCATGACGAGGAGCTGCACGATGACTGAACAGGCAACAACAACCGATg                                                                    <  2:171845/251‑1 (MQ=255)
                                                                                                                                                                       tttttATTTCCGAAACGTACCTCAGCAGGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTATAATTAAAATGGAAATTGTTTTTGATTTTGCATTTTAAATGAGTAGTCTTAGTTGTGCTGAACGAAAAGAGCACAACGATCCTTCGTTCACAGTGGGGAAGTTTTCGGATCCATGACGAGGAGCTGCACGATGACTGAACAGGCAACAACAACCGATGAACTGGCTTTCACaa                                                     <  1:47108/249‑1 (MQ=255)
                                                                                                                                                                         ttgaTTTCCGAAACGTACCTCAGCAGGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTATAATTAAAATGGAAATTGTTTTTGATTTTGCATTGTAAATGAGTAGTCTTAGTTGTGCTGAACGAAAAGAGCACAACGATCCTTCGTTCACAGTGGGGAAGTTTTCGGATCCATGACGAGGAGCTGCACGATGACTGAACAGGCAACAACAACCGATGAACTGGCTTTCACAAGGc                                                  <  1:34646/247‑1 (MQ=255)
                                                                                                                                                                            aTTTCCGAAACGTACCTCAGCAGGAGAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTATAATTAAAATGGACATTGTTTTTGATTTTGCATTTTAAATGAGTAGTCTTAGTTGTGCTGAACGAAAAGAGCACAACGATCCTTCGTTCACAGTGGGGAAGTTTTCGGATCCATGACGAGGAGCTGAACGATGACTGAACAGGCAACAACCACCGATGAACTGGCTTTcacacagccgt                                               >  1:431927/1‑243 (MQ=255)
                                                                                                                                                                                   aaaCGTACCTCAGCAGGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTATAATTAAAATGGAAATTGTTTTTGATTTTGCATTTTAAATGAGTAGTCTTAGTTGTGCTGAACGAAAAGAGCACAACGATCCTTCGTTCACAGTGGGGAAGTTTTCGGATCCATGACGAGGAGCTGCACGATGACTGAACAGGCAACAACAACCGATGAACTGGCTTTCACAAGGCCGTATGGCg                                         >  2:179713/1‑249 (MQ=255)
                                                                                                                                                                                                     gAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTATAATTAAAATGGAAATTGTTTTTGATTTTGCATTTTAAATGAGTAGTCTTAGTTGTGCTGAACGAAAAGAGCACAACGATCCTTCTCTCACAGGGGGGAAGTTTTCGGATCCATGACGAGGAGCTGCACGATGACTGAACAGGCAACAACAACCGATTAACTGGCTTTCACAAGGCCGTATGGCGAGCAGGAGAAGCAAATTCtt                     <  2:243443/251‑1 (MQ=255)
                                                                                                                                                                                                                        tGTTATCAACAAGTTATCAAGTATTTTTAATTAAAATGGAAATTGTTTTTGATTTTGCATTTTAAATGAGTAGTCTTAGTTGTGCTGAACGAAAAGAGCACAACGATCCTTCGTTCACAGTGGGGAAGTTTTCGGATCCATGACGAGGAGCTGCACGATGACTGAACAGGCAACAACAACCGATGAACTGGCTTTCACAAGGCCGTATGGCGAGCAGGAGAAGCAAATTCTTACTGCCGAAGCGGTAGAAt  >  2:113026/1‑251 (MQ=255)
                                                                                                                                                                                                                                          aaGTATTTATAATTAAAATGGAAATTGTTTTTGATTTTGCATTTTAAATGAGTAGTCTTAGTTGTGCTGAACGAAAAGAGCACAACGATCCTTCGTTCACAGTGGGGAAGTTTTCGGATCCATGACGAGGAGCTGCa                                                                                                  >  1:483414/1‑137 (MQ=255)
                                                                                                                                                                                                                                          aaGTATTTATAATTAAAATGGAAATTGTTTTTGATTTTGCATTTTAAATGAGTAGTCTTAGTTGTGCTGAACGAAAAGAGCACAACGATCCTTCGTTCACAGTGGGGAAGTTTTCGGATCCATGACGAGGAGCTGCa                                                                                                  <  2:483414/137‑1 (MQ=255)
                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                
AGCTGGCGTAGTCACGGTAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACGATCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGGGTGCCTTTTTTATTTCCGAAACGTACCTCAGCAGGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTTTAATTAAAATGGAAATTGTTTTTGATTTTGCATTTTAAATGAGTAGTCTTAGTTGTGCTGAACGAAAAGAGCACAACGATCCTTCGTTCACAGTGGGGAAGTTTTCGGATCCATGACGAGGAGCTGCACGATGACTGAACAGGCAACAACAACCGATGAACTGGCTTTCACAAGGCCGTATGGCGAGCAGGAGAAGCAAATTCTTACTGCCGAAGCGGTAGAAT  >  NC_000913/4215105‑4215571

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑