Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,133,252 C→T P213S (CCG→TCG)  metF → 5,10‑methylenetetrahydrofolate reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,133,2520CT92.3% 63.3 / NA 26P213S (CCG→TCG) metF5,10‑methylenetetrahydrofolate reductase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (9/15);  minor base G (0/2);  total (9/17)
Fisher's exact test for biased strand distribution p-value = 5.29e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGCAGATTTCGATATCTCCGTGGCGGCGTATCCGGAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTACTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGG‑AAATTATTCCGGGAATTTTGCCGGTATCTAACTTT‑AAACAGGCGAAGAAATTTGCCGATATGACCAA‑CGTGCGTATTCCGGCGTGGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCG‑AAACCCGCAAACTGGTTGGCGCGAATATTGCCATGGATATGGTGAAGATTTTAAGCCGTGAAGGAGTGAAAGATTTCCACTTCTATACGCTTAACCGTGCTGAAATGAGTTACGCGATTTGCCATACGCTGGGGGTTC  >  NC_000913/4133041‑4133492
                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                   
ggCAGATTTCGATATCTCCGTGGCGGCGTATCCGGAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTACTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGG‑AAATTATTCCGGGAATTTTGTCGGTATCTAACTTT‑AAACAGGCGAAGAAATTTGCCGat                                                                                                                                                                                                              <  2:132529/250‑1 (MQ=255)
           atatCTCCGTGGCGGCGTATCCGGAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTACTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGG‑AAATTATTCCGGGAATTTTGTCGGTATCTAACTTT‑AAACAGGCGAAGAAATTTGCCGATATGACCAA‑CGTg                                                                                                                                                                                                 <  1:269037/251‑1 (MQ=255)
                    gggcggcGTATCCGGAAGTGCACCCGGAAGCAAAAAGCGCGCAGGCGGATTGGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTACTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGG‑AAATTATTCCGGGAATTTTGTCGGTATCTAACTGT‑AAACAGGCGAAGAAATTTGCCGATATGACCAA‑CGTGCGTATTCCg                                                                                                                                                                                        <  2:112677/250‑1 (MQ=255)
                       cggcgTATCCGGAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTACTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCAGTGTTGTGGGAATTTTTTCCTGGA‑TTTTGTCGGTGTTTAACTTTTAAAAATCGGAAGAA‑TTTTTCGGTATGACCAA‑CATGCGGATTCCGGCg                                                                                                                                                                                     >  2:95927/1‑251 (MQ=255)
                       cggcgTATCCGGAAGTTCACCCGGAAGCAAAAAGCGCACAGGCGGATTTGCTTAAGCTGAAACGCAAAGGGGATGCCGGATCCAACCGCGCGATTACTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTTATGTGG‑AAATTATTCCGGGAATTTTGTCGGTATCTAACTTT‑AAACAGGCGAAGAAATTTGCCGATATGACCAA‑CGTGCGTATTCCGGct                                                                                                                                                                                     <  2:9357/251‑2 (MQ=255)
                        ggcgTATCCGGAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTACTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGG‑AAATTATTCCGGGAATTTTGTCGGTATCTAACTTT‑AAACAGGCGAAGAAATTTGCCGATATGACCAA‑CGTGCGTATTCCGGCGt                                                                                                                                                                                    <  1:175077/251‑1 (MQ=255)
                                    aGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTACTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGGTGTGG‑GAATTATTCCCGGAATTTTTTCGGGTTCTAAATTT‑AAACAGGCGAAGAAATTTGCCGATATGACCAA‑CGGTCGTATTCCGGCGGGGATGGCGCaa                                                                                                                                                                         >  1:44875/1‑250 (MQ=255)
                                            cggcaagaaaaaGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTTGATGCCGGAGCCAACCGCGCGATTCCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGG‑AAATTATTCCGGGAATTTTGTCGGTATCTAACTTT‑AAACAGGCGAAGAAATTTGCCGATATGACCAA‑CGTGCGTATTCCGGCGTGGACGGCGCAAATGTTCGAc                                                                                                                                                                <  2:139853/244‑1 (MQ=255)
                                               aaGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTACTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCCTTTATGTGG‑GAATTATTTCGGGGATTTTTTTGGTATCTTACTTT‑TAAAAGGGGAAGAAAATTTCCGGTATTACCCA‑CGGGGGGATTCCGGCGTGGGTGGCGCCAATGtttggcggg                                                                                                                                                             >  1:304112/1‑244 (MQ=255)
                                               aaGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTACTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGG‑AAATTATTCCGGGAATTTTGTCGGTATCTAACTTT‑AAACAGGCGAAGAAATTTGCCGATATGACCAA‑CGTGCGTATTCCGGCGTGGATGGCGCAAATGTTCGACGGt                                                                                                                                                             <  2:117367/251‑1 (MQ=255)
                                                                                              gacgCCGGAGCCAACCGCGCGATTACTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTGCGTGAGCGCTGTGTATCGGCGGGCATTGATGTGG‑AAATTAGTCCGGGAATTTTGGCGGTATCTAACTTT‑AAACAGGCGAAGAAATTTGCCGATATGACCAA‑CGTTCGTATTCCGGCGTGGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCG‑AAACCCGCAAGCTGGTTGGCGCGAATATTGc                                                                                                             <  2:392403/248‑1 (MQ=255)
                                                                                                    ggAGCCAACCGCGCGATTACTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCCTTGATGTGG‑GAATTTTTTCGGGGATTTTTTTGGGATTTTACTTT‑AAAAAGGGGAAGGAATATTACGGTATGACCAAACG‑GCGTATTTCTGCGTGGGTGGGGCAAAAGTTTGACGGGCTGGATGATGGTGCCGCAAACCCCCAAAC‑GGTTGGGGCGCATAttttcctggg                                                                                                       >  2:446396/1‑243 (MQ=255)
                                                                                                       gCCAACCGCGCGATTACTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGG‑AAATTATTCCGGGAATTTTGTCGGTATCTAACTTT‑AAACAGGCGAAGAAATTTGCCGATATGACCAA‑CGTGCGTATTCCGGCGTGGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCG‑AAACCCGCAAACTGGTTGGCGCGAATATTGCCATGGatat                                                                                                    <  1:199426/251‑1 (MQ=255)
                                                                                                        ccAACCGCGCGATTACTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGG‑GAATTATTCCGGGGATTTTTTTGGGATCTTAATTT‑TAACAAGCGGAGAAATTTTCCGGTATGACCCA‑CGTGCGGATTTCCGCGGGGGTGGCGCAAAAGTTCGGCGGGCTGGGAGTTTATGCCG‑GAAACCGCAAAACGGTTGGCGCGCATATTGCCCTGGGTATg                                                                                                   >  1:363795/1‑251 (MQ=255)
                                                                                                                 cgATTACTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGG‑AAATTATTCCGGGAATTTTGTCGGTATCTAACTTT‑AAACAGGCGAAGAAATTTGCCGATATGACCAA‑CGTGCGTATTCCGGCGTGGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCG‑AAACCCGCAAACTGGTTGGCGCGAATATTGCCATGGATATGGTGAAGAtt                                                                                          <  2:386056/251‑1 (MQ=255)
                                                                                                                   aTTACTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGG‑GAATTATTTCGGGGATTTTTTCGGTATCTAACTTT‑TAAAAAGCGAAGAAATTTTCCGCTATGACCAA‑CGTGCGTATTTCGGCGGGGGTGGGGCCAAAGTTCGACGGTCTGGGTGATGATGCCG‑AAAACCGCCAACTGGGTGGCGCGGATATTGTCATGGGTATGGTGGAGAtttt                                                                                        >  1:476416/1‑251 (MQ=255)
                                                                                                                                    cGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGG‑AAATTATTCCGGGAATTTTGTCGGTATCTAACTTT‑AAACAGGCGAAGAAATTTGCCGATATGACCAA‑CGTGCGTATTCCGGCGTGGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCG‑AAACCCGCAAACTGGTTGGCGCGAATATTGCCATGGATATGGTGAAGATTTTAAGCCGTGAAGGAGTGa                                                                       <  2:452100/251‑1 (MQ=255)
                                                                                                                                                                   cGCTGTGTATCGGCGGGCATTGATGTGG‑AAATTATTCCGGGAATTTTGTCGGTATCTAACTTT‑AAACAGGCGAAGAAATTTGCCGATATGACCAA‑CGTGCGGATTCTGGCGTGGATGGCGCAAATGTTCGACg                                                                                                                                                               <  1:125926/133‑1 (MQ=255)
                                                                                                                                                                    gCTGTGTATCGGCGGGCATTGATGTGG‑AAATTATTCCGGGAATTTTGTCGGTATCTAACTTT‑AAACAGGCGAAGAAATTTGCCGATATGACCAA‑CGTGCGTATTCCGGCGTGGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCG‑AAACCCGCAAACTGGTTGGCGCGAATATTGCCATGGATATGGTGAAGATTTTAAGCCGTGAAGGAGTGAAAGATTTCCACTTCTATACGCTTAACCGTGCt                                       <  2:25252/251‑1 (MQ=255)
                                                                                                                                                                    cctttgtATTGGCGTGCATTGATGTTG‑AAATTATTCCGGGAATTTTGGCGGTATCTAACTTT‑AAACAGGCGAAGAAATTTGCCGATATGACCAA‑CGTGCGTATTCCGGCGTGGATGGCGCAAATGTTCGACTGTATGGATGATGATGCCG‑AAACCCGCAAACTGGTTGGCGCGAATATTGCCATGGATATGGTGAAGATTTTAAGCCGTGAAGGAGTGAAAGAGTTCCACTTCTATACGCTTAACCGTGCt                                       <  2:102492/247‑1 (MQ=255)
                                                                                                                                                                            gcggcggGCATTGATGTGG‑AAATTATTCCGGGAATTTTGTCGGTATCTAACTTT‑AAACAGGCGAAGAAATTTGCCGATATGACCAA‑CGTGCGTATTCCGGCGTGGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCG‑AAACCCGCAAACTGGTTGGCGCGAATATTGCCATGGATATGGTGAAGATTTTAAGCCGTGAAGGAGTGAAAGATTTCCACTTCTATACGCTTAACCGTGCTGAAATGAg                               <  2:442530/250‑1 (MQ=255)
                                                                                                                                                                             cggcggGCATTGATGTGG‑AAATTATTCCGGGAATTTTGTCGGTATCTAACTTT‑AAACAGGCGAAGAAATTTGCCGATATGACCAA‑CGTGCGTATTCCGGCGTGGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCG‑AAACCCGCAAACTGGTTGGCGCGAATATTGCCATGGATATGGTGAAGATTTTAAGCCGTGAAGGAGTGAAAGATTTCCACTTCTATACGCTTAACCGTGCTGAAATGAGt                              <  1:446396/251‑1 (MQ=255)
                                                                                                                                                                                    ccttaaTGTGG‑AAATTATTCCGGGAATTTTGTCGGTATCTACCTTT‑CAACAGGCGAAGAAATTTGCCGATATGACCAA‑CGTGCGTATTCCGGCGTGGATGGAGCAAATGTTCGACGGTATGGATGATGATGCCG‑AAACACGCAAACTGGATGGCGCGATTATTGCCATGGATATGGAGAAGATTTTAAGCCGTGAAGGAGTGACAGATTTCCACTTCTATACGATTAACCGTTCTGAATTGAGTTAcgcg                        >  2:137914/6‑250 (MQ=255)
                                                                                                                                                                                        gATGTGG‑AAATTATGCCGGGAATTTTGTCGGTTTCTAACTTT‑AAACAGGCGAAGAAATTTGCCGATATGACCTA‑CGTGCGTATTCCGGCGTGGATGGCGCAAATGGGCGACGGTCTGGATGATGATGCCG‑AAACCCGCAAACTGTTTGGCGCGAATATTGCCATGGATATGGTGAAGATTTTAAGCCGTGAAGGAGTGAAAGATTTCCACTTCTATACGCTTAACCGTGCTGAAATGAGTTACGCGATTTg                   <  1:453757/251‑1 (MQ=255)
                                                                                                                                                                                                    tattCAGGGAATTTTGTAGGTATCTAACTTT‑AAACAGGCGAAGAAATTTGCCGATATGACCAA‑CGTGCGTATTCCGGCGTGGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCG‑AAACCCGCAAACTGGTTGGCGCGAATATTGCCATGGATATGGTGAAGATTTTAAGCCGTGAAGGAGTGAAAGATTTCCACTTCTATACGCTTAACAGTGCTGAAATGAGGTACGCGATTTGCCATACGTTgg        >  2:224882/1‑251 (MQ=255)
                                                                                                                                                                                                          gggAATTTTGTCGGTATCTAACTTT‑AAACAGGCGAAGAAATTTGCCGATATGACCAA‑CGTGCGTATTCCGGCGTGGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCG‑AAACCCGCAAACTGGTTGGCGCGAATATTGCCATGGATATGGTGAAGATTTTAAGCCGTGAAGGAGTGAAAGATTTCCACTTCTATACGCTTAACCGTGCTGAAATGAGTTACGCGATTTGCCATACGCTGGGGGTTc  >  1:43445/1‑251 (MQ=255)
                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                   
GGCAGATTTCGATATCTCCGTGGCGGCGTATCCGGAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTACTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGG‑AAATTATTCCGGGAATTTTGCCGGTATCTAACTTT‑AAACAGGCGAAGAAATTTGCCGATATGACCAA‑CGTGCGTATTCCGGCGTGGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCG‑AAACCCGCAAACTGGTTGGCGCGAATATTGCCATGGATATGGTGAAGATTTTAAGCCGTGAAGGAGTGAAAGATTTCCACTTCTATACGCTTAACCGTGCTGAAATGAGTTACGCGATTTGCCATACGCTGGGGGTTC  >  NC_000913/4133041‑4133492

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑