Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,931,779	T→C	F155S (TTT→TCT)	kup →	K(+):H(+) symporter Kup

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,931,779	0	T	C	100.0%	77.9 / NA	24	F155S (TTT→TCT)	kup	K(+):H(+) symporter Kup
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (13/11);  total (13/11)

AGATAACGCCGGTGAGGGGGGGATCCTGACGTTGATGTCGCTTGCCGGGCGTAATACGTCGGCGCGAACCACATCAATGCTGGTGATTATGGGGCTAATCGGCGGCAGCTTTTTCTATGGTGAAGTCGTCATAACACCCGCTATTTCGGTGATGTCAGCCATTGAAGGTCTGGAAATCGTCGCCCCGCAGCTGGATACCTGGATAGTTCCCCTCTCAATTATCGTTCTCACTTTATTATTTATGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGTGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTACGGGG  >  NC_000913/3931540‑3931996                                                                                                                                                                                                                                                |                                                                                                                                                                                                                          aGATAACGCCGGTGAGGGGGGGATCCTGACGGTGATGTCGCTTGCCGTGCGTAAAACGACGGCGAGAACCACATCAATGCTGGTGATTATAGGGCTAATCGGGGGCAGCTTGTTCTATGGAGAAGTAGTCATAAAACCCGCATTTTCGGTGATGTCAGCCAGTGAAGGTCTGGAAATCGGCGCCCCACAGCTGTTTACATGGATAGTTCCACTCTCACTTATCGTTCTCGCTTTATTATCTTTGATTCaa                                                                                                                                                                                                                 >  1:288120/1‑250 (MQ=255)                              cGTTGATGTCGCTTGCAGGGCCTAATACGTCGGCGCGAACCACATCACTGCTGGTGATTATGGGGCTAATCGGCGGCAGCTTTTTCTATGGTGAAGTCGTCATAACACCCGCTATTTCGGTGATGTCAGCCATTGAAGGTCTGGCAATCGTCGCCCCGCAGCTGGATACCTGGATAGTTCCCCTCTCAATTATCGTTCTCCCTTTATTATCTATGTTTCAAAAACATGGCCCGGCTATGGTCGGTAAGCTg                                                                                                                                                                                   >  2:25986/1‑251 (MQ=255)                                        gCTTGCCGGGCGTAATACGTCGGCGCGAACCACATCAATGCTGGTGATTATTGGGCTAATCGGCGGCAGCTTTTTCTATGGTGAAGTCGTCATAACACCCGCTATTTCGGTGATGTCAGCCATTGAAGGTCTGGAAATCGTCGCCCCGCAGCTGGATACCTGGATAGTTCCCCTCTCAATTATCGTTCTCACTTTATTATCTATGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGa                                                                                                                                                                         <  2:123722/251‑1 (MQ=255)                                            gCCGGGCGTAATACGTCCGCGCGACCCACATCAATGCTGGTGATTATGGGGCTAATCGGCGGCAGCTTTTTCTATGGTGAAGTCGTCATAACTCCCGCTATTTCGGTGATGTCAGCCATTGAAGGTCTGGAAATCGTCGCCCCGCAGCTGGATACCTGGATAGTTCCCCTCTCAATTATCGTTCTCACTTTATTATCTATGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCAt                                                                                                                                                                     <  2:20164/251‑1 (MQ=255)                                             ccGGGCGTAATACGTCGGCGCGAACCACATCAATGCTGGTGATTATGGGGCTAATCGGCGGCAGCTTTTTCTATGGTGAAGTCGTCATAACACCCGCTATTTCGGTGCTGTCAGCCATTGAAGGTCTGGAAATCGTCGCCCCGCAGCTGGATACCTGGCTAGTTCCCCTCTCAATTATCGTTCTCACTTTATTATCTATGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGCTCATg                                                                                                                                                                    >  1:449068/1‑251 (MQ=255)                                             ccGGGCGTAATACGTCGGCGCGAACCACATCAATGCTGGTGATTATGGGGCTAATCGGCGGCAGCTTTTTCTATGGTGAAGTCGTCATAACACCCGCTATTTCGGTGATGTCAGCCATTGAAGGTCTGGAAATCGTCGCCCCGCAGCTGGATACCTGGATAGTTCCCCTCTCAATTATCGTTCTCACTTTATTATCTATGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATg                                                                                                                                                                    >  1:331511/1‑251 (MQ=255)                                                  cGTAATACGTCGGCGCGAACCACATCAATGCTGGTGATTATGGGGCTAATCGGCGGCAGCTTTTTCTATGGTGAAGTCGTCATAACACCCGCTATTTCGGTGATGTCAGCCATTGAAGGTCTGGAAATCGTCGCCCCGCAGCTGGATACCTGGATAGTTCCCCTCTCAATTATCGTTCTCACTTTATTATCTATGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGAc                                                                                                                                                               >  1:334560/1‑251 (MQ=255)                                                                     ccACATCAATGCTGGTGATTATGGGGCTAATCGGCGGCAGCTTTTTCTATGGTGAAGTCGTCATAACACCCGCTATTTCGGTGATGTCAGCCATTGAAGGTCTGGAAATCGTCGCCCCGCAGCTGGATACCTGGATAGTTCCCCTCTCAATTCTCGTTCTAACTTTATTATCTATGATTCAAAAACATGGCCCGGCTATGGTCGGTACGCTGTTTGCGCCGATCATTCTGCACTGGTTTTTGATTCTGGcc                                                                                                                                            >  2:192745/1‑250 (MQ=255)                                                                        caTCAATGCTGGTGATTATGGGGCTAATCGGCGGCAGCTTTTTCTATGGGGAAGTCGTCATAACACCCGCTATTTCGGTGATGTCAGCCATTGAAGGTCTGGAAATCGTCGCCCCGCAGCTGGATACCTGGATAGTTCCCCTCTCAATTATCGTTCTCACTTTATTATCTATGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGACTCTGGCAGGa                                                                                                                                         <  2:29342/251‑1 (MQ=255)                                                                           cAATGCTGGTGATTATGGGGCTAATCGGCGGCAGCTTTTTCTATGGTGAAGTCGTCATAACACCCGCTATTTCGGTGATGTCAGCCATTGAAGGTCTGGAAATCGTCGCCCCGCAGCTGGATACCTGGATAGTTCCCCTCTCAATTATCGTTCTCACTTTATTATCTATGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACt                                                                                                                                       <  2:193347/250‑1 (MQ=255)                                                                                           ggggCTAATCGGCGGCAGCTTTGTCTATGGTGAAGGCGTCATAACACCCGCTATTTCGGTGATGTCAGCCATTGAAGGGCTGGAAATCGTCGCCCCGCAGCTGGATACCTGGATAGTTCCCCTCTCAATTATCGGTCTCACTTTATTATCTATGATTCAAAATCATGTCACGGCTATGGTCTGTAAGCTGTGTGCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGTTACGTAGCatta                                                                                                                      <  2:429651/251‑1 (MQ=255)                                                                                                                             gtcgtcATAACACCCGCTATTTCGGTGATGTCAGCCATTGAAGGTCTGGAAATCGTCGCCCCGCAGCTGGATACCTGGATAGTTCCCCTCTCAATTATCGTTCTCACTTTATTATCTATGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGTGCTGCATGCGCTGAATcc                                                                                    >  1:44660/1‑251 (MQ=255)                                                                                                                                  cATAACACCCGCTATTTCGGTGATGTCAGCCATTGAAGGTCTGGAAATCGTCGCCCCGCAGCTGGATACCTGGATAGTTCCCCTCTCAATTATCGTTCTCACTTTATTATCTATGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGTGCTGCATGCGCTGAATCCAAtgt                                                                               <  2:334560/251‑1 (MQ=255)                                                                                                                                     aaCACCCGCTATTTCGGTGATGTCAGCCATTGAAGGTCTGGAAATCGTCGCCCCGCAGCTGGATACCTGGATAGTTCCCCTCTCAATTATCGTTCTCACTTTATTATCTATGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCCGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGTGCTGCATCCGCTGAATCCAATGTggg                                                                            >  1:457417/1‑251 (MQ=255)                                                                                                                                               aTTTCGGTGATGTCAGCCATTGAAGGTCTGGAAATCGTCGCCCCGCAGCTGGATACCTGGATAGTTCCCCTCTCAATTATCGTTCTCACTTTATTATCTATGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGTGCTGCATGCGCTGAATCCAATGTGGGCGGTGCAttt                                                                  >  1:355954/1‑251 (MQ=255)                                                                                                                                                               ccATTGAAGGTCTGGAAATCGTCGCCCCGCAGCTGGATACCTGGATAGTTCCCCTCTCAATTATCGTTCTCACTTTATTATCTATGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGTGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACaa                                                   >  1:69141/1‑250 (MQ=255)                                                                                                                                                                 agtGAAGGTCTGGAAATCGTCGCCCCGCAGCTGGATACCTGGATAGTTCCCCTCTCAATTATCGTTCTCACTTTATTATCTATGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGTGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAAc                                                <  2:233742/249‑1 (MQ=255)                                                                                                                                                                            ggAAATCGTCGCCCCGCAGCTGGATACCTGGATAGTTCCCCTCTCAATTATCGTTCTCACTTTATTATCTATGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGTGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGtt                                            <  2:262510/244‑1 (MQ=255)                                                                                                                                                                            ggAAATCGTCGCCCCGCAGCTGGATACCTGGATAGTTCCCCTCTCAATTATCGTTCTCACTTTATTATCTATGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGTGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGtt                                            >  1:262510/1‑244 (MQ=255)                                                                                                                                                                              aaaTCGTCGCCCCGCAGCTGGATACCTGGATAGTTCCCCTCTCAATTATCGTTCTCACTTTATTATCTATGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGTGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTAtt                                   <  1:51287/251‑1 (MQ=255)                                                                                                                                                                               aaTCGTCGCCCCGCAGCTGGATACATGGATAGTTCCCCTCTCAATTATCGTTCTCACTTTATTATCTATGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCGGGTCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGTGCTGAATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTg                                  >  1:147075/1‑251 (MQ=255)                                                                                                                                                                                     cgCCCCGCAGCTGGATACCTGGATAGTTCCCCTCTCAATTATCGTTCTCACTTTCTTATCTATGATTCAAAAACATGGCACGGCTCTGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGTGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCAtt                              >  2:219498/1‑249 (MQ=255)                                                                                                                                                                                                             aGTTCCCCTCTCAATTATCGTTCTCACTTTATTATCTATGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGTGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTACgg    <  2:44660/251‑1 (MQ=255)                                                                                                                                                                                                                tCCCCTCTCAATTATCGTTCTCACTTTATTATCTATGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGTGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTACgggg  <  1:29131/250‑1 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                          AGATAACGCCGGTGAGGGGGGGATCCTGACGTTGATGTCGCTTGCCGGGCGTAATACGTCGGCGCGAACCACATCAATGCTGGTGATTATGGGGCTAATCGGCGGCAGCTTTTTCTATGGTGAAGTCGTCATAACACCCGCTATTTCGGTGATGTCAGCCATTGAAGGTCTGGAAATCGTCGCCCCGCAGCTGGATACCTGGATAGTTCCCCTCTCAATTATCGTTCTCACTTTATTATTTATGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGTGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTACGGGG  >  NC_000913/3931540‑3931996

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑