Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,719,429	G→A	intergenic (‑385/‑49)	yiaF ← / → yiaG	DUF3053 domain‑containing protein YiaF/putative DNA‑binding transcriptional regulator YiaG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,719,429	0	G	A	100.0%	139.4 / NA	44	intergenic (‑385/‑49)	yiaF/yiaG	DUF3053 domain‑containing protein YiaF/putative DNA‑binding transcriptional regulator YiaG
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (15/29);  total (15/29)

TGTTGTTTCATTGTTAAAAAATGATAGTGCCGTTGCCTTTTTTGTAGCCAGAGATTTACGCCTTCGCAGAAGGTTAGATTTTCGAAATCTTTACAAAACAGGAGTGGTAAGAGATATATCGGCATTTATCAGATCGTTAGGTGGCTATAAGTCACGCTAAATGACAGGCTGAATCGAATCATAGCCAGAGCATGCCCTGACTTCACCCCGCTGTGTCTGCTTTTCCCGACTATTCTTAATGAGCTTCGATGCAATTCACGATCCCGCAGTGTGATTTGAGGAGTTTTCAATGGAATATAAAGATCCAATGCATGAGCTGTTGAGCAGCCTGGAACAGATTGTTTTTAAAGATGAAACGCAGAAAATTACCCTGACGCACAGAACAACGTCCTGTACCGAAATTGAGCAGTTACGAAAAGGGACAGGATTAAAAATCGATGATTTCGCCCGGGTTTTGGGCGTATCAGTCGCCATGGTAAAGGAAT  >  NC_000913/3719189‑3719673                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                     aggtgttTGATTGTTAAACAATGATAGTACCGTTGCCTTTTTTGTATCCAGAGATTTACGCCTTCGCAGAAGGTTAGATTTTCGACCTCTTTACAAAACAGGAGTGGTAAGAGATATATCGGCATTTATCAGATCGTTAGGTGGCTATAAGTCAAGCTAAATGACAGGCTGAATCGATTCATAGCAAGAGAATGCCATGACTTCACCCCGATGGGTCTGCTTTTCCCGGCCATTCTTAATAAGCATCGATg                                                                                                                                                                                                                                            >  2:77097/4‑251 (MQ=255)             ggtAAAAAATGATAGTGCCGTTGCCTTTTTTGTAGCCAGAGATTTACGCCATCGCAGAAGGTTAGATTTTCGAAATCTTTACAAAACAGGAGTGGTAAGAGATATATCGGCATTTATCAGATCGTTAGGTGGCTATAAGTCACGCTAAATGACAGGCTGAATCGAATCATAGCCAGAGCATGCCCTGACTTCACCCCGCTGTGTCTGCTTTTCCCGACTATTCTTAATAAGCTTCGATGCAATTCACGATc                                                                                                                                                                                                                                <  2:312311/249‑1 (MQ=255)                             gCCGTTGCCTTTCTTGTAGCCAGAGAGTTACGCCATCGCAGAAGGGTAGATTTTCGAAATCTTTACAAAACAGGAGTGGTAAGAGATATATCGGCATTTATCAGATCGTTAGGTGGCTATAAGTCACGCTAAATGACAGGCTGAATCGAATCATAGCCAGAGCATGCCCTGACTTCACCCCGCTGTGTCTGCTTTTCCCGACTATTCTTAATAAGCTTCGATGCAATTCACGATCCCGCAGTGTGATTTg                                                                                                                                                                                                                 <  2:328052/250‑1 (MQ=255)                               cGTTGCCTTTTTTGTAGCCAGAGATTTACGCCTTCGCAGAAGGTTAGATTTTCGAAATCTTTACAAAACAGGAGTGGTAAGAGATATATCGGCATTTATCAGATCGTTAGGTGGCTATAAGTCACGCTAAATGACAGGCTGAATCGAATCATAGCCAGAGCATGCCCTGACTTCACCCCGCTGTGTCTGCTTTTCCCGACTATTCTTAATAAGCTTCGATGCAATTCACGATCCCGCAGTGTGATTTgagg                                                                                                                                                                                                              >  1:404320/1‑251 (MQ=255)                                    ccTTTTTTGTAGCCAGAGATTTACGCCTTCGCAGAAGGTTAGATTTTCGAAATCTTTACAAAACAGGAGTGGTAAGAGATATATCGGCATTTATCAGATCGTTAGGTGGCTATAAGTCACGCTAAATGACAGGCTGAATCGAATCATAGCCAGAGCATGCCCTGACTTCACCCCGCTGTGTCTGCTTTTCCCGACTATTCTTAATAAGCTTCGATGCAATTCAGGCTCCCGCAGTGTGATTTGAGGGGttt                                                                                                                                                                                                         >  2:335991/1‑251 (MQ=255)                                        tcttggaGCCCGAGATTTTCGCCTTCTCAGATGGTGAGATTTTCGAAAGCTTGACAAAACAGGAGTGGTAAGAGATATATCGGCAGTTATCAGATCGTTAGGTGGCTAGAAGTCCCGCTAAATGACAGGCTGAATCGGATCATATCCAGAGCATTCCCTGACTTCTCCCCGCGGTGTCTGCTTTTCCCGGCTATTCTTAAAAAGCTGCGATGCAATTCACGAACCCGCAGTGTGATTTGAGGAGtctt                                                                                                                                                                                                        <  2:254233/242‑4 (MQ=255)                                                           cGCCTTCGCAGAAGGTTAGATTTTCGAAATCTTTACAAAACAGGAGTGGTAAGAGATATATCGGCATTTATCAGATCGTTAGGTGGCTATAAGTCACGCTAAATGACAGGCTGAATCGAATCATAGCCAGAGCATGCCCTGACTTCACCCCGCTGTGTCTGCTTTTCCCGACTATTCTTAATAAGCTTCGATGCAATTCACGATCCCGCAGTGTGATTTGAGGAGTTTTCAATGGAATATAAAGATCCAAt                                                                                                                                                                                  <  2:84428/251‑1 (MQ=255)                                                               ttCGCAGAAGGTTAGATTTTCGAAATCTTTACAAAACAGGAGTGGTAAGAGATATATCGGCATTTATCAGATCGTTAGGTGGCTATAAGTCACGCTAAATGACAGGCTGAATCGAATCATAGCCAGAGCATGCCCTGACTTCACCCCGCTGTGTCTGCTTTTCCCGACTATTCTTAATAAGCTTCGATGCAATTCACGATCCCGCAGTGTGATTTGAGGAGTTTTCAATGGAATATAAAGATCCAATGCAt                                                                                                                                                                              >  1:232537/1‑251 (MQ=255)                                                                          ttAGATTTTCGAAATCTTTACAAAACAGGAGTGGTAAGAGATATATCGGCATTTATCAGATCGTTAGGTGGCTATAAGTCACGCTAAATGACAGGCTGAATCGAATCATAGCCAGAGCATGCCCTGACTTCACCCCGCTGTGTCTGCTTTTCCCGACTATTCTTACTAAGCTTCGATGCACTTCACGATCCCGCAGCGGGATTTGAGGAGTTTTCAATGGCATATAAAGATCCAATGCATGAGCTGTTg                                                                                                                                                                     >  2:12953/1‑249 (MQ=255)                                                                                  tCGAAATCTTTACAAAACAGGAGTGGTAAGAGATATATCGGCATTTATCAGATCGTTAGGTGGCTATAAGTCACGCTAAATGACAGGCTGAATCGAATCATAGCCAGAGCATGCCCTGACTTCACCCCGCTGTGTCTGCTTTTCCCGACTATTCTTAATACGCTTCGATGCAATTCACGATCCCGCAGTGTGATTTGAGGAGTTTTCAATGGAATATAAAGAACCAATGCATGAGCTGTGGAGCAGCCTg                                                                                                                                                            <  1:3850/250‑1 (MQ=255)                                                                                  tCGAAATCTTTACAAAACAGGAGTGGTAAGAGATATATCGGCATTTATCAGATCGTTAGGTGGCTATAAGTCACGCTAAATGACAGGCTGAATCGAATCATAGCCAGAGCATGCCCTGACTTCACCCCGCTGTGTCTGCTTTTCCCGACTATTCTTAATAAGCTTCGATGCAATTCACGATCCCGCAGTGTGATTTGAGGAGTTTTCAATGGAATATAAAGATCCAATGCATGAGCTGTTGAGCAGCCTg                                                                                                                                                            <  1:335991/250‑1 (MQ=255)                                                                                  tCGAAATCTTTACAAAACAGGAGTGGTAAGAGATATATCGGCATTTATCAGATCGGTAGGTGGCTATAAGTCACGCTAAATGACAGGTTGAATCGAATCATAGCCAGAGCATGCCCTGACTTCACCTCGCTGTGTCTGTTTTTCCCGACTATGCTTAATAAGCTTCGATGCAATTCACGATCCCGCAGTGTGATTTGAGGAGTTTTCAATGGAATATAAAGATCCAATGCATGAGCTGTTGAGCAGCCTg                                                                                                                                                            <  1:448572/250‑1 (MQ=255)                                                                                   cGAAATCTTTACAAAACAGGAGTGGTAAGAGATATATCGGCATTTATCAGATCGTTAGGTGGCTATAAGTCACGCTAAATGACAGGCTGAATCGAATCATAGCCAGAGCATGCCCTGACTTCACCCCGCTGTGTCTGCTTTTCCCGACTATTCTTAATAAGCTTCGATGCAATTCACGATCCCGCAGTGTGATTTGAGGAGTTTTCAATGGAATATAAAGATCCAATGCATGAGCTGTTGAGCAGCCTgg                                                                                                                                                           <  1:12953/250‑1 (MQ=255)                                                                                      aaTCTTTACAAAACAGGAGTGGTAAGAGATATATCGGCATTTATCAGATCGTTAGGTGGCTATAAGTCACGCTAAATGACAGGCTGAATCGAATCATAGCCAGAGCATGCCCTGACTTCACCCCGCTGTGTCTGCTTTTCCCGACTATTCTTAATAAGCTTCGATGCAATTCACGATCCCGCAGTGTGATTTGAGGAGTTTTCAATGGAATATAAAGATCCAATGCATGAGCTGTTGAGCAGCCTGGAAc                                                                                                                                                        >  1:208325/1‑250 (MQ=255)                                                                                              cAAATCAGGAGTGTTAAGAGACATATCGGAATTTATCAGAACGTGAGGTGGCTATAAGTCGCGCTAAAGGTCAGGCTGAATCGAATCATAGCCAGAGCATGCCCTGACTTCACCCCTCTGTGTCTGCTTGTCCCGACTATGCTTAATAAGCTTCGATGCAATTCACGCTCCCGCAGTGTGATTTGAGGAGTTTTCAATGGAATATAAAGATCCAATGCATGATCCGTTGAGCTGCCTGGAACATATTGttt                                                                                                                                               <  1:177717/251‑1 (MQ=255)                                                                                                       aGTGGTAAGAGATATATCGGCATTTATCAGATCGTTAGGTGGCTATAAGTCACGCTAAATGACAGGCTGAATCGAATCATAGCCAGAGCATGCCCTGACTTCACCCCGCTGTGTCTGCTTTTCCCGACTATTCTTAATAAGCTTCGATGCAATTCACGATCCCGCAGTGTGATTTGAGGAGTTTTCAATGGAATATAAAGAGCCAATGCATTAGCTGTTGAGCAGCCTGGAACAGATTGTTTTTAAAGATg                                                                                                                                      <  1:413705/251‑1 (MQ=255)                                                                                                             aaGAGATATATCGGCATTTATCAGATCGTTAGGTGGCTATAAGTCACGCTAAATGACAGGCTGAATCGAATCATAGCCAGAGCATGCCCTGACTTCACCCCGCTGTGTCTGCTTTTCCCGACTATTCTTAATAAGCTTCGATGCAATTCACGATCCCGCAGTGTGATTTGAGGAGTTTTCAATGGAATATAAAGATCCAATGCATGAGCTGTTGAGCAGCCTGGAACAGATTGTTTTTAAAGATGAAACGc                                                                                                                                >  1:324508/1‑251 (MQ=255)                                                                                                             aaGAGATATATCGGCATTTATCAGATCGTTAGGTGGCTATAAGTCACGCTAAATGACAGGCTGAATCGAATCATAGCCAGAGCATGCACTGACTTCACCCCGCTGTGTCTGCTTTTCCCGACTATTCTTAATAAGCTTCGATGCAATTCACGATCCCGCAGTGTGCTTTGAGGAGTTTTCAATGGAATATAAAGATCCAATGCATGAGCTGTTGAGCAGCCTGGAACAGATTGTTTTTCAAGATGAAACGc                                                                                                                                >  1:317099/1‑251 (MQ=255)                                                                                                                           cATTTATCAGATCGTTAGGTGGCTATAAGTCACGCTAAATGACAGGCTGAATCGAATCATAGCCAGAGCATGCCCTGACTTCACCCCGCTGTGTCTGCTTTTCCCGACTATTCTTAATAAGCTTCGATGCAATTCACGATCCCGCAGTGTGATTTGAGGAGTTTTCAATGGAATATAAAGATCCAATGCATGAGCTGTTGAGCAGCCTGGAACAGATTGTTTTTAAAGATGAAACGCAGAAAATTACCCTg                                                                                                                  <  2:404320/251‑1 (MQ=255)                                                                                                                              ttacccgttCGTTAGGTGGCTATAAGTCACGCTAAATGACAGGCTGATTCTAATCATAGCCAGTTCATGCCCTGACTTCACCCCGCTTTGTCTTCTTTTCCCGACTATTCTTGATAAGCTTCGATGCAATTCACGATCCCGCAGTGTGATTTGAGGAGTTTTCAATGGAATATAAAGATCCAATGCATGAGCTGATGAGCAGCCTGGAACAGATTGTTTTTAAAGATGAAACGGAGAAAATTACTCTGAc                                                                                                                <  2:155026/242‑1 (MQ=255)                                                                                                                              ttATCAGATCGTTAGGTGGCTATAAGTCACGCTAAATGACAGGCTGAATCGAATCATAGCCAGAGCATGCCCTGACTTCACCCCGCTGTGTCTGCTTTTCCCGACTATTCTTAATAAGCTTCGATGCAATTCACGATCCCGCAGTGTGATTTGAGGAGTTTTCAATGGAATATAAAGATCCAATGCATGAGCTGTTGAGCAGCCTGGAACAGATTGTTTTTAAAGATGAAACGCAGAAAATTACCCTGAc                                                                                                                <  2:288329/250‑1 (MQ=255)                                                                                                                               tATCAGATCGTTAGGTGGCTATAAGTCACGCTAAATGACAGGCTGAATCGAATCATAGCCAGAGCATGCCCTGACTTCACCCCGCTGTGTCTGCTTTTCCCGACTATTCTTAATAAGCTTCGATGCAATTCACGATCCCGCAGTGTGATTTGAGGAGTTTTCAATGGAATATAAAGATCCAATGCATGAGCTGTTGAGCAGCCTGGAACAGATTGTTTTTAAAGATGAAACGCAGAAAATTACCCTGACg                                                                                                               <  1:44001/250‑1 (MQ=255)                                                                                                                                  cAGATCGTTAGGTGGCTATAAGTCACGCTAAATGACAGGCTGAATCGAATCATAGCCAGAGCATGCCCTGACTTCACCCCGCTGTGTCTGCTTTTCCCGACTATTCTTAATAAGCTTCGATGCAATTCACGATCCCGCAGTGTGATTTGAGGAGTTTTCAATGGAATATAAAGATCCAATGCATGAGCTGTTGAGCAGCCTGGAACAGATTGTTTTTAAAGATGAAACGCAGAAAATTACCCTGACGcaca                                                                                                           <  1:277607/251‑1 (MQ=255)                                                                                                                                  cAGATCGTTAGGTGGCTATAAGTCACGCTAAATGACAGGCTGAATCGAATCATAGCCAGAGCATGCCCTGACTTCACCCCGCTGTGTCTGCTTTTCCCGACTATTCTTAATAAGCTTCGATGCAATTCACGATCCCGCAGTGTGATTTGAGGAGTTTTCAATGGAATATAAAGATCCAATGCATGAGCTGTTGAGCAGCCTGGAACAGATTGTTTTTAAAGATGAAACGCAGAAAATTACCCTGACGcaca                                                                                                           <  1:325144/251‑1 (MQ=255)                                                                                                                                        gTTAGGTGGCTATAAGTCACGCTAAATGACAGGCTGAATCGAATCATAGCCAGAGCATGCCCTGACTTCACCCCGCTGTGTCTGCTTTTCCCGACTATTCTTAATAAGCTTCGATGCAATTCACGATCCCGCAGTGTGATTTGAGGAGTTTTCAATGGAATATAAAGATCCAATGCATGAGCTGTTGAGCAGCCTGGAACAGATTGTATTTAAAGATGAAACGCAGAAAATTACCCTGACGCACAGaacaa                                                                                                     <  2:259867/251‑1 (MQ=255)                                                                                                                                             gTGGCTATAAGTCACGCTAAATGACAGGCTGAATCGAATCATAGCCAGAGCATGCCCTGACTTCACCCCGCTGTGTCTGCTTTTCCCGACTATTCTTAATAAGCTTCGATGCAATTCACGATCCCGCAGTGTGATTTGAGGAGTTTTCAATGGAATATAAAGATCCAATGCATGAGCTGTTGAGCAGCCTGGAACAGATTGTTTTTAAAGATGAAACg                                                                                                                                 <  1:169844/218‑1 (MQ=255)                                                                                                                                             gTGGCTATAAGTCACGCTAAATGACAGGCTGAATCGAATCATAGCCAGAGCATGCCCTGACTTCACCCCGCTGTGTCTGCTTTTCCCGACTATTCTTAATAAGCTTCGATGCAATTCACGATCCCGCAGTGTGATTTGAGGAGTTTTCAATGGAATATAAAGATCCAATGCATGAGCTGTTGAGCAGCCTGGAACAGATTGTTTTTAAAGATGAAACg                                                                                                                                 >  2:169844/1‑218 (MQ=255)                                                                                                                                              tGGCTATAAGTCACGCTAAATGACAGGCTGAATCGAATCATAGCCAGAGCATGCCCTGCCTTCACCCCGCTGTGTCTGCTTTTCCCGACTATTCTTAATAAGCTTCGATGCAATTCACGATCCCGCAGTGTGATTTGAGGAGTTTTCAATGGAATATAAAGATCCAATGCATGAGCTGTTGAGCAGCCTGGAAAAGATTGTTTTTAAAGATGAAACGCAGAAAATTACCCTGACGCACAGAACAACGTCCt                                                                                               >  2:443869/1‑251 (MQ=255)                                                                                                                                              tGGCTATAAGTCACGCTAAATGACAGGCTGAATCGAATCATAGCCAGAGCATGCCCTGACTTCACCCCGCTGTGTCTGCTTTTCCCGACTATTCTTAATAAGCTTCGATGCAATTCACGATCCCGCAGTGTGATTTGAGGAGTTTTCAATGGAATATAAAGATCCAATGCATGAGCTGTTGAGCAGCCTGGAACAGATTGTTTTTAAAGATGAAACGCAGAAAATTACCCTg                                                                                                                  <  2:436191/232‑1 (MQ=255)                                                                                                                                              tGGCTATAAGTCACGCTAAATGACAGGCTGAATCGAATCATAGCCAGAGCATGCCCTGACTTCACCCCGCTGTGTCTGCTTTTCCCGACTATTCTTAATAAGCTTCGATGCAATTCACGATCCCGCAGTGTGATTTGAGGAGTTTTCAATGGAATATAAAGATCCAATGCATGAGCTGTTGAGCAGCCTGGAACAGATTGTTTTTAAAGATGAAACGCAGAAAATTACCCTg                                                                                                                  >  1:436191/1‑232 (MQ=255)                                                                                                                                               ggCTATAAGTCACGCTAAATGACAGGCTGAATCGAATCATAGCCAGAGCATGCCCTGACTTCACCCCGCTGTGTCTGCTTTTCCCGACTATTCTTAATAAGCTTCGATGCAATTCACGATCCCGCAGTGTGATTTGAGGAGtttt                                                                                                                                                                                                        >  1:163157/1‑145 (MQ=255)                                                                                                                                                                           gaatcgaatcATAGCCAGAGCATGCCCTGACTTCACCCCGCTGTGTCTGCTTTTCCCGACTATTCTTAATAAGCTTCGATGCAATTCACGATCCCGCAGTGTGATTTGAGGAGTTTTCAATGGAATATAAAGATCCAATGCATGAGCTGTTGAGCAGCCTGGAACAGATTGTTTTTAAAGATGAAACGCAGAAAATTACCCTGACGCACAGAACAACGTCCTGTACCGAACTTGAGCAGTTACGAAAAggg                                                                  >  2:164650/1‑251 (MQ=255)                                                                                                                                                                             atcgaatcATAGCCAGAGCATGCCCTGACTTCACCCCGCTGTGTCTGCTTTTCCCGACTATTCTTAATAAGCTTCGATGCAGTTCACGATCCCGCAGTGTGATTTGAGGAGTTTTCAATGGAATATAAAGATCCAATGCATGAGCTGTTGAGCAGCCTGGAACAGATTGTTTTTAAAGATGAAACGCAGAAAATTACCCTGACGCACAGAACAACGTCCTGTACCGAAATTGAGCAGTTACGCAAAGGGAc                                                                >  1:95095/1‑251 (MQ=255)                                                                                                                                                                                           agagCATGCCCTGACTTCACCCCGCTGTGTCTGCTTTTCCCGACTATTCTTAATAAGCTTCGATGCAATTCACGATCCCGCAGTGTGATTTGAGGAGTTTTCAATGGAATATAAAGATCCAATGCATGAGCTGTTGAGCAGCCTGGAACAGATTGTTTTTAAAGATGAAACGCAGAAAATTACCCTGACGCACAGAACAACGTCCTGTACCGAAATTGAGCAGTTACGAAAAGGGACAGGATTAAAAATCg                                                  <  2:169212/251‑1 (MQ=255)                                                                                                                                                                                             gtcttGCCCTGACTTAACCCCGCTGGTTCTGCTTTTCCCGACTATTCTTAATAAGCTTCGATGCAATTCACGATCCTGCAGTGTGATTTTAGTAGTTTTCAATTGAATATAAAGATCCAATGCATGCGCGGGTGAGCAGCCTTGACCATATTGTTATCAAATATGAAACGCAGAAACTTCCCCTGTCGCACAGAACAACGTCCTGTACCGAAATTGAGCAGTTACGAAAATGGACAGGacgaatattcgat                                                <  2:447776/247‑13 (MQ=255)                                                                                                                                                                                                       gACTTCCCCCCGCTGTGTCTGCTTTTCCCGACTATTCTTAATAAGCTTCGATGCAATTCACGATCCCGCAGTGTGATTTGAGGAGTTTTCAATGGAATATAAAGATCCAATGCATGAGCTGTTGAGCAGCCTGGAACAGATTGTTTTTAAAGATGAAACGCAGAAAATTACCCTGACGCACAGAACAACGTCCTGTACCGAAATTGAGCAGTTACGAAAAGGGACAGGATTAAAAATCGATGATTTCGccc                                      <  1:458870/251‑1 (MQ=255)                                                                                                                                                                                                       gACTTCACCCCGCTGTGTCTGCTTTTCCCGACTATTCTTAATAAGCTTCGATGCAATTCACGATCCCGCAGTGTGATTTGAGGAGTTTTCAATGGAATATAAAGATCCAATGCATGAGCTGTTGAGCAGCCTGGAACAGATTGTTTTTAAAGATGAAACGCAGAAAATTACCCTGACGCACAGAACAACGTCCTGTACCGAAATTGAGCAGTTACGAAAAGGGACAGGATTAAAAATCGATGATTTCGccc                                      <  1:164650/251‑1 (MQ=255)                                                                                                                                                                                                           tccacccGCTTTGTCTGCGTTTCACGACTATTCTTAATAAGCTTCGATGCAATTCACGATCCCGCAATGTTATTTGAGGAGTTTGCAATGGAATATAAAGATCCAATGCATGATCTGTTGAGCAGCCTGGAACAGATTGTTTTTAAAGATTAAGCGCAGAAAATTACCCTGACGCACAGAACAACGTCCTGTACCGAAATTGAGTAGTTACGAAAAGGGACATGATTAAAAAACTATGATTTCGCCCggt                                   <  2:161041/246‑2 (MQ=255)                                                                                                                                                                                                           tCACCCCGCTGTGTCTGCTTTTCCCGACTATTCTTAATAAGCTTCGATGCAATTCACGATCCCGCAGTGTGATTTGAGGAGTTTTCAATGGAATATAAAGATCCAATGCATGAGCTGTTGAGCAGCCTGGAACAGATTGTTTTTAAAGATGAAACGCAGAAAATTACCCTGACGCACAGAACAACGTCCTGTACCGAAATTGAGCAGTTACGAAAAGGGACAGGATTAAAAATCGATGATTTCGCCCggg                                   <  1:87566/250‑1 (MQ=255)                                                                                                                                                                                                                                 cccGACTATTCTTAATAAGCTTCGATGCAATTCACGATCCCGCAGTGTGATTTGAGGAGTTTTCAATGGAATATAAAGATCCAATGCATGAGCTGTTGAGCAGCCTGGAACAGATTGTTTTTAAAGATGAAACGCAGAAAATTACCCTGACGCACAGAACAACGTCCTGTACCGAAATTGAGCAGTTACGAAAAGGGACAGGATTAAAAATCGATGATTTCGCCCGGGTTTTGGGCGTATCAGTCGCCAt             <  1:468123/250‑1 (MQ=255)                                                                                                                                                                                                                                 cccGACTATTCGTAATAAGCTTCGATGCAATTCACGATCCCGCAGTGTGATTTGAGGAGTTTTCAATGGAATATAAAGATCCAATGCATGAGCTGTTGAGCAGCCTGGAACAGATTGTTTTTAAAGATGAAACGCAGAAAATTACCCTGACGCACAGAACAACGTCCTGTACCGAAATTGAGCAGTTACGAAAAGGGACAGGATTAAAAATCGATGATTTCGCCCGGGTTTTGGGCGTATCAGTCGCCAt             <  2:128530/250‑1 (MQ=255)                                                                                                                                                                                                                                  ccGACTATTCTTAATAAGCTTCGATGCAATTCACGATCCCGCCGTGTGATTTGAGGAGTTTTCAATGGAATATAAAGa                                                                                                                                                                                        >  2:48915/1‑78 (MQ=255)                                                                                                                                                                                                                                  ccGACTATTCTTAATAAGCTTCGATGCAATTCACGATCCCGCAGTGTGATTTGAGGAGTTTTCAATGGAATATAAAGa                                                                                                                                                                                        <  1:48915/78‑1 (MQ=255)                                                                                                                                                                                                                                            ttAATAAGCTTCGATGCAATTCACGATCCCGCAGTGTGATTTGAGGAGTTTTCAATGGAATATAAATATCCAATGCATGAGCTGTTGAGCAGCCTGGAACAGATTGTTTTTAAAGATGAAACGCAGAAAATTACCCTGACGCACAGAACAACGTCCTGTACCGAAATTGAGCAGTTACGAAAAGGGACAGGATTAAAAATCGATGATTTCGCCCGGGTTTTGGGCGTATCAGTCGCCATGGTAAAGGAAt  <  1:202349/250‑1 (MQ=255)                                                                                                                                                                                                                                             taataaGCTTCGATGCAATTCACGATCCCGCAGTGTGATTTGAGGAGTTTTCAATGGAATATAAAGATCCAATGCATGAGCTGTTGAGCAGCCTGGAACAGATTGTTTTTAAAGATGAAACGCAGAAAATTACCCTGACGCACAGAACAACGTCCTGTACCGAAATTGAGCAGTTACGAAAAGGGACAGGATTAAAAATCGATGATTTCGCCCGGGTTTTGGGCGTATCAGTCGCCATGGTAAAGGAAt  <  1:324385/249‑1 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                     TGTTGTTTCATTGTTAAAAAATGATAGTGCCGTTGCCTTTTTTGTAGCCAGAGATTTACGCCTTCGCAGAAGGTTAGATTTTCGAAATCTTTACAAAACAGGAGTGGTAAGAGATATATCGGCATTTATCAGATCGTTAGGTGGCTATAAGTCACGCTAAATGACAGGCTGAATCGAATCATAGCCAGAGCATGCCCTGACTTCACCCCGCTGTGTCTGCTTTTCCCGACTATTCTTAATGAGCTTCGATGCAATTCACGATCCCGCAGTGTGATTTGAGGAGTTTTCAATGGAATATAAAGATCCAATGCATGAGCTGTTGAGCAGCCTGGAACAGATTGTTTTTAAAGATGAAACGCAGAAAATTACCCTGACGCACAGAACAACGTCCTGTACCGAAATTGAGCAGTTACGAAAAGGGACAGGATTAAAAATCGATGATTTCGCCCGGGTTTTGGGCGTATCAGTCGCCATGGTAAAGGAAT  >  NC_000913/3719189‑3719673

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑