Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,517,171	G→A	L46F (CTT→TTT)	damX ←	cell division protein DamX

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,517,171	0	G	A	92.2%	134.2 / 3.0	51	L46F (CTT→TTT)	damX	cell division protein DamX
Reads supporting (aligned to +/- strand):  ref base G (2/2);  new base A (16/31);  total (18/33)
Fisher's exact test for biased strand distribution p-value = 6.07e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.81e-01

TTTAGCGCAGAACCGATACCGATGATCAACAGCAGTAGAACCAGAATGCCGACGCCCATCATCATATACTGACGAGAAGCGGGTTTACTGGCTGCTTTTTTGCGCTTACGCGGACGACGCTCTACGCGCTCTTCATCCACGGTTTCATCTTCGGATTCGTCAATTTCTTCTTCGATTTCCGGTTCCTCATTGCGCTCTTTTTGCGCACGAGTCGGACGGCGATCGTCAGTGTCATCAAGTTCAATATCATCAAAATTGATCTGCGGTTCGCCACGTTCAGTACGCTCAGAACGTTCAGAAGATTGACGAGAACGACCAGTACGACGATCGCTGGGATCGGGTTTCAGCTCGTCTTCTGGTTTGAATTCATCCATTTAACACCCCACTAAAAGGTTAATGCTTACCACGTTGCAATTAACCTGAAGCTAATAGACCGCTTGATAAGCGGCCTGACCTTTCTTGTTGTTACGCTGATTGACAATC  >  NC_000913/3516933‑3517415                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                     ttgaGCGCATAACCGATACCGATGATCAACCACAGTAGAACCAGTATGCCTGCGCCCATCATCATATACTGACGAGAAGCGGGTTTACTGGCTGCTTTTTTGCGCTTACGCGGACGACGCTCTACGCGCTCTTCTTCCACGGTTTCATCTTCGGATTCGTCAATTTCTTCTTCGATTTCCGGTTCCTCATTGCGCTCTTTTTGCGCACGAGTCGGACGGCGATCGTCAGTGTCATCAAATTCAATatcat                                                                                                                                                                                                                                           <  2:113042/247‑1 (MQ=255)       gcAGAACCGATACCGATGATCAACAGCAGTAGAACCAGAATGCCGACGCCCATCATCATATACTGACGAGAAGCGGGTTTACTGGCTGCTTTTTTGCGCTTACGCGGACGACGCTCTACGCGCTCTTCATCCACGGTTTCATCTTCGGATGCGTCAATTTCTTCTTCGATTTCCGGTTCCTCATTGCGCTCTTTTTGCGCACGAGTCGGACGGCGATCGTCAGTGTCATCAAATTCAATATCATCAAAAtt                                                                                                                                                                                                                                    <  1:279645/251‑1 (MQ=255)        cAGAACCGATACCTAGGATCAACAGCAGTAGAACCAGAATGCCGACGCCCATCATCATATACTGACGAGAAGCGGGTTTACTGGCTGCTTTTTTGCGCTTACGCGGACGACGCTCTACGCGCTCTTCATCCACGGTTTCATCTTCGGATTCGTCAATTTCTTCTTCGATTTCCGGTTCCTCATTGCGCTCTTTTTGCGCACGAGTCGGACGGCGATCGTCAGTGTCATCAAATTCAATATCATCAAAAttt                                                                                                                                                                                                                                   <  2:194883/251‑2 (MQ=255)          gAACCGATACCGATGATCAACAGCAGTAGAACCAGAATGCCGACGCCCATCATCATATACTGACGAGAAGCGGGTTTACTGGCTGCTTTTTTGCGCTTACGCGGACGACGCTCTACGCGCTCTTCATCCACGGTTTCATCTTCGGATTCGTCAATTTCTTCTTCGATTTCCGGTTCCTCATTGCGCTCTTTTTGCGCACGAGTCGGACGGCGATCGTCAGTGTCATCAAATTCAATATCATCAAAATTGAt                                                                                                                                                                                                                                 <  1:350039/251‑1 (MQ=255)           aaCCGATACCGATGATCAACAGCAGTAGAACCAGAATGCCGACGCCCATCATCATATACTGACGAGAAGCGGGTTTACTGGCTGCTTTTTTGCGCTTACGCGGACGACGCTCTACGCGCTCTTCATCCACGGTTTCAGCTTCGGATTCGTCAATTTCTTCTTCGATTTCCGGTTCCTCATTGCGCTCTTTTTGCGCACGAGTCGGACGGCGATCGTCAGTGTCATCAAATTCAATATCATCAAAATTGATc                                                                                                                                                                                                                                <  1:260618/251‑1 (MQ=255)               gagaccgatGATTAACAGCAGTAGAACCAGAATGCCGACGCCCATCATCATATCCTGACGAGAAGCGGGTTTACTGGCTGCTTTTTTGCGCTTACGCGGACGACGCTCTACGCGCTCTTCATCCACGGTTTCATCTTCGGATTCGTCAATTTCTTCTTCGATTTCCGGTTCCTCATTGCGCTCTTTTTGCGCACGAGTCGGACGGCGATCGTCAGTGTCATCAAATTCAATATCATCAAAATTGATCTGCg                                                                                                                                                                                                                            <  2:172665/248‑1 (MQ=255)                     gatgatCAACAGCAGTAGAACCAGAATGCCGACGCCCATCATCATATACTGACGAGAAGCGGGTTTACTGGCTGCTTTTTTGCGCTTACGCGGACGACGCTCTACGCGCTCTTCATCCACGGTTTCATCTTCGGATTCGTCAATTTCTTCTTCGATTTCCGGTTCCTCATTGCGCTCTTTTTGCGCACGAGTCGGACGGCGATCGTCAGTGTCATCACATTCAATATCATCAAAATTGATCTGCGGTTCGc                                                                                                                                                                                                                      >  2:140922/1‑251 (MQ=255)                      atgatCAACAGCAGTAGAACCAGAATGCCGACGCCCATCATCATATACTGACGAGAAGCGGGTTTACTGGCTGCTTTTTTGCGCTTACGCGGACGACGCTCTACGCGCTCTTCATCCACGGTTTCATCTTCGGATTCGTCAATTTCTTCTTCGATTTCCGGTTCCTCATTGCGCTCTTTTTGCGCACGAGTCGGACGGCGATCGTCAGTGTCATCAAATTCAATATCATCAAAATTGATCTGCGGTTCGcc                                                                                                                                                                                                                     >  1:134413/1‑251 (MQ=255)                            aaCAGCAGTAGAACCAGAATGCCGACGCCCATCATCATATACTGACGAGAAGCGGGTTTACTGGCTGCTTTTTTGCGCTTACGCGGACGACGCTCTACGCGCTCTTCATCCACGGTTTCATCTTCGGATTCGTCAATTTCTTCTTCGATTTCCGGTTCCTCATTGCGCTCTTTTTGCGCACGAGTCGGACGGCGATCGTCAGTGTCATCAAATTCACTATCATCAAAATTGATCTGCGGTTCGCCACGTTc                                                                                                                                                                                                               >  2:415240/1‑251 (MQ=255)                                gcagTAGAACCAGAATGCCGACGCCCATCATCATATACTGACGAGAAGCGGGTTTACTGGCTGCTTTTTTGCGCTTACGCGGACGACGCTCTACGCGCTCTTCATCCACGGTTTCATCTTCGGATTCGTCAATTTCTTCTTCGATTTCCGGTTCCTCATTGCGCTCTTTTTGCGCACGAGTCGGACGGCGATCGTCAGTGTCATCAAATTCAATATCATCAAAATTGATCTGCGGTTCGCCACGTTCAGTa                                                                                                                                                                                                           <  1:350538/251‑1 (MQ=255)                                          cAGAATGCCGACGCCCATCATCATATACTGACGAGAAGCGGGTTTACTGGCTGCTTTTTTGCGCTTACGCGGACGACGCTCTACGCGCTCTTCATCCACGGTTTCATCTTCGGATTCGTCAATTTCTTCTTCGATTTCCGGTTCCTCATTGCGCTCTTTTTGCGCACGAGTCGGACGGCGATCGTCAGTGTCATCAAATTCAATATCATCAAAATTGATCTGCg                                                                                                                                                                                                                            >  2:45136/1‑224 (MQ=255)                                          cAGAATGCCGACGCCCATCATCATATACTGACGAGAAGCGGGTTTACTGGCTGCTTTTTTGCGCTTACGCGGACGACGCTCTACGCGCTCTTCATCCACGGTTTCATCTTCGGATTCGTCAATTTCTTCTTCGATTTCCGGTTCCTCATTGCGCTCTTTTTGCGCACGAGTCGGACGGCGATCGTCAGTGTCATCAAATTCAATATCATCAAAATTGATCTGCg                                                                                                                                                                                                                            <  1:45136/224‑1 (MQ=255)                                            gAATGCCGACGCCCATCAGCATATTCTGACGAGAAGCGGGTTTACTGGCTGCTTTTTTGCGCTTACGCTGACGACGCGCTACGCGCTCTTCAACCACGGTTTCATCTTCTGATTCGTCAATTTCTTCTCCGATTTCCGGTTCCTCGTTGCGCGCTTGTTGCGCACGAGTCGGACTGCGATCGTCAGTGTCATCAAATTCAATATCATCAAAACTGATCTGCGGTTCGCCACGTTCAGTACGCTCAGAACGt                                                                                                                                                                                               <  1:455143/251‑1 (MQ=255)                                                                         cGAGAAGCGGGTTTACTGGCTGCTTTTTTGCGCTTACGCGGACGACGCTCTACGCGCTCTTCATCCACGGTTTCATCTTCGGATTCGTCAATTTCTTCTTCGATTTCCGGTTCCTCATTGCGCTCTTTTTGCGCACGAGTCGGACGGCGATCGTCAGTGTCATCAAATTCAATATCATCAAAATTGATCTGCGGTTCGCCACGTTCAGTACGCTCAGAACGTTCAGAAGATTGACGAGAACGACCAGTacg                                                                                                                                                                  <  1:388349/251‑1 (MQ=255)                                                                              aGCGGGTTTACTGGCTGCTTTTTTGCGCTTACGCGGACGACGCTCTACGCGCTCTTCATCCACGGTTTCATCTTCGGATTCGTCAATTTCCTCTTCGATTTCCGGTTCCTCATTGCGCTCTTTTTGCGCACGAGTCGGACGGCGATCGTCAGTGTCATCAAATTCAATATCATCAAAATTGATCTGCGGTTCGCCACGTGCAGTACGCTCAGAACGTTCAGAAGATTGACGAGAACGACCAGTACGACGAt                                                                                                                                                             <  1:47714/251‑1 (MQ=255)                                                                                      tACTGGCTGCTTTTTTGCGCTTACGCGGACGACGCTCTACGCGCTCTTCATCCACGGTTTCATCTTCGGATTCGTCAATTTCTTCTTCGATTTCCGGTTCCTCATTGCGCTCTTTTTGCGCACGAGTCGGACGGCGATCGTCAGTGTCATCAAATTCAATATCATCAAAATTGATCTGCGGTTCGCCACGTTCAGTACGCTCAGAACGTTCAGAAGATTGACGAGAACGACCAGTACGACGATCGCTggg                                                                                                                                                      <  1:277338/250‑1 (MQ=255)                                                                                                   tttGCGCTTACGCGGACGACGCTCTACGCGCTCTTCATCCACGGTTTCATCTTCGGATTCGTCAATTTCTTCTTCGATTTCCGGTTCCTCATTGCGCTCTTTTTGCGCACGAGTCGGACGGCGATCGTCAGTGTCATCAAATTCAATATCATCAAAATTGATCTGCGGTTCGCCACGTTCAGTACGCTCAGAACGTTCAGAAGATTGACGAGAACGACCAGTACGACGATCGCTGGGATCGGGTTTCAGCt                                                                                                                                        >  1:359130/1‑251 (MQ=255)                                                                                                     tGCGCTTACGCGGACGACGCTCTACGCGCTCTTCATCCACGGTTTCATCTTCGGATTCGTCAATTTCTTCTTCGATTTCCGGTTCCTCATTGCGCTCTTTTTGCGCACGAGTCGGACGGCGATCGTCAGTGTCATCAAGTTCAATATCATCAAAATTGATCTGCGGTTCGCCACGTTCAGTACGCTCAGAACGTTCAGAAGATTGACGAGAACGGCCAGTACGACGATCGCTGGGATCGGGTTTCAGCTCg                                                                                                                                      >  2:68927/1‑251 (MQ=255)                                                                                                               cgGACGACGCTCTACGCGCTCTTCATCCACGGTTTCATCTGCGGATTCGTCAATTTCTTCTTCGATTTCCGGTTCCTCATTGCGCTCTTTTTGCGCACGAGTCGGACGGCGATCGTCAGTGTCATCAAATTCAATATCATCAAAATTGATCTGCGGTTCGCCACGTTCAGTACGCTCAGAACGTTCAGAAGATTGACGAGAACGACCAGTACGACGATCGCTGGGATCGGGTTTCAGCGCGTCTTCTGGtt                                                                                                                            <  2:144796/251‑1 (MQ=255)                                                                                                                    gacgCTCTACGCGCTCTTCATCCACGGTTTCATCTTCGGATTCGTCAATTTCTTCTTCGAGTTCCGGTTCCTCATTGCGCTCTTTTTGCGCACGAGTCGGACGGCGATCGTCAGTGTCATCAAATTCAATATCATCAAAATTGATCTGCGGTTCGCCACGTTCAGTACGCTCAGAACGTTCAGAAGATTGACGAGAACGACCAGTACGACGATCGCTGGGATCGGGTTTCAGCTCGTCTTCTGGTTTGAAt                                                                                                                       <  2:171174/251‑1 (MQ=255)                                                                                                                          ctACGCGCTCTTCATCCACGGTTTCATCTTCGGATTCGTCAATTTCTTCTTCGATTTCCGGTTCCTCATTGCGCTCTTTTTGCGCACGAGTCGGACGGCGATCGTCAGTGTCATCAAATTCAATATCATCAAAATTGATCTGCGGTTCGCCACGTTCAGTACGCTCATAACGTTCAGAAGATTGACGAGAACGACCAGTACGACGATCGCTGGGATCGGGTTTCAGCTCGTCTTCTGGTTTGAATTCAt                                                                                                                   <  1:451619/249‑1 (MQ=255)                                                                                                                            aCGCGCTCTTCATCCACGGTTTCATCTTCGGATTCGTCAATTTCTTCTTCGATTTCCGGTTCCTCATTGCGCTCTTTTTGCGCACGAGTCGGACGGCGATCGTCAGTGTCATCAAATTCAATATCATCAAAATTGATCTGCGGTTCGCCACGTTCAGTACGCTCAGAACGTTCAGAAGATTGACGAGAACGACCAGTACGACGATCGCTGGGATCGGGTTTCAGCTCGTCTTCTGGTTTGAATTCATcc                                                                                                                 <  1:476175/249‑1 (MQ=255)                                                                                                                             cgcgCTCTTCATCCACGGTTTCATCTTCGGATTCGTCAATTTCTTCTTCGATTTCCGGTTCCTCATTGCGCTCTTTTTGCGCACGAGTCGGACGGCGATCGTCAGTGTCATCAAATTCAATATCATCAAAATTGATCTGCGGTTCGCCACGTTCAGTACGCTCAGAACGTTCAGAAGATTGACGAGAACGACCAGTACGACGATCGCTGGGATCGGGTTTCAGCTCGTCTTCTGGTTTGAATTCATCCAtt                                                                                                              >  1:181212/1‑251 (MQ=255)                                                                                                                                   tttCATCCTCGGTTTCATCCTCGGATTCGTCAATTGCTTCCTCGATTTCCTGTTCCTCATTGCGCTCTTTTTGCGCACGAGTCGGACGGCGATCGTCAGTGTCATCAAATTCAATATCATCAAAATTGATCTGCGGTTCGCCACGTTCAGTACGCTCAGAACGTTCAGAAGATTGACGAGAACGACCAGTACGACGATCGCTGGGATCGGGTTTCAGCTCGTCTTCTGGTTTGAATTCATCCATTTAac                                                                                                          <  2:424267/248‑1 (MQ=255)                                                                                                                                      cATCCTCGGTTTCATCTTCGGATTCGTCAATTTCTTCTTCGATTTCCGGTTCCTCATTGCGCTCTTTTTGCGCACGAGTCGGACGGCGATCGTCAGTGTCATCAAATTCAATATCATCAAAATTGATCTGCGGTTCGCCACGTTCAGTACGCTCAGAACGTTCAGAAGATTGACGAGAACGACCAGTACGACGATCGCTGGGATCGGGTTTCAGCTCGTCTTCTGGTTTGAATTCATCCATTTAACAcccc                                                                                                     <  2:2422/251‑1 (MQ=255)                                                                                                                                         ccACGGTTTCATCTTCGGATTCGTCAATTTCTTCTTCGATTTCCGGTTCCTCATTGCGCTCTTTTTGCGCACGAGTCGGACGGCGATCGTCAGTGTCATCAAATTCaa                                                                                                                                                                                                                                                 <  2:255540/108‑1 (MQ=255)                                                                                                                                         ccACGGTTTCATCTTCGGATTCGTCAATTTCTTCTTCGATTTCCGGTTCCTCATTGCGCTCTTTTTGCGCACGAGTCGGACGGCGATCGTCAGTGTCATCAAATTCaa                                                                                                                                                                                                                                                 >  1:255540/1‑108 (MQ=255)                                                                                                                                             ggTTTCATCTTCGGATTCGTCAATTTCTTCTTCGATTTCCGGTTCCTCATTGCGCTCTTTTTGCGCACGAGTCGGACGGCGATCGTCAGTGTCATCAAATTCAATATCATCAAAATTGATCTGCGGTTCGCCACGTTCAGTACGCTCAGAACGTTCAGAAGATTGACGAGAACGACCAGTACGACGATCGCTGGGATCGGGTTTCAGCTCGTCTTCTGGTTTGAATTCATCCATTTAACACCCCACTaaaa                                                                                              <  1:140922/251‑1 (MQ=255)                                                                                                                                                    tCTTCGGATTCGTCAATTTCTTCTTCGATTTCCGGTTCCTCATTGCGCTCTTTTTGCGCACGAGTCGGACGGCGATCGTCAGTGTCATCAAATTCAATATCATCAAAATTGATCTGCGGTTCGCCACGTTCAGTACGCTCAGAACGTTCAGAAGATTGACGAGAACGACCAGTACGACGATCGCTGGGATCGGGTTTCAGCTCGTCTTCTGGTTTGAATTCATCCATTTAACACCCCACTAAAAGGTTAAt                                                                                       >  1:135155/1‑251 (MQ=255)                                                                                                                                                       tCGGATTCGTCAATTTCTTCTTCGATTTCCGGTTCCTCATTGCGCTCTTTTTGCGCACGAGTCGGACGGCGATCGTCAGTGTCATCAAATTCAATATCATCAAAATTGATCTGCGGTTCGCCACGTTCAGTACGCTCAGAACGTTCAGAAGATTGACGAGAACGACCAGTACGACGATCGCTGGGATCGGGTTTCAGCTCGTCTTCTGGTTTGAATTCATCCATTTAACACCCCACTAAAAGGTTAATGc                                                                                     <  1:441278/250‑1 (MQ=255)                                                                                                                                                              cGTCAATTTCTTCTTCGATTTCCGGTTCCTCATTGCGCTCTTTTTGCGCACGAGTCGGACGGCGATCGTCAGTGTCATCAAATTCAATATCATCAAAATTGATCTGCGGTTCGCCACGTTCAGTACGCTCAGAACGTTCAGAAGATTGACGAGACCGACCAGTACGACGATCGCTGGGATCGGGTTTCAGCTCGTCTTCTGGTTTGAATTCATCCATTTAACACCCCACTAAAAGGTTAATGCTTACCACg                                                                             >  2:387444/1‑251 (MQ=255)                                                                                                                                                                               aTTTCCGGTTCCTCATTGCGCTCTTTTTGCGCACGAGTCGGACGGCGATCGTCAGTGTCATCAAATTCAATATCATCAAAATTGATCTGCGGTTCGCCACGTTCAGTACGCTCAGAACGTTCAGAAGATTGACGAGAACGACCAGTACGACGATCGCTGGGATCGGGTTTCAGCTCGTCTTCTGGTTTGAATTCATCCATTTAACACCCCACTAAAAGGTTAATGCTTACCACGTTGCAATTAACCTGAAg                                                            <  2:22877/251‑1 (MQ=255)                                                                                                                                                                                   ccGGTTCCTCATTGCGCTCTTTTTGCGCACGAGTCGGACGGCGATCGTCAGTGTCATCAAGTTCAATATCATCAAAATTGATCTGCGGTTCGCCACGTTCAGTACGCTCAGAACGTTCAGAAGATTGACGAGAACGACCAGTACGACGATCGCTGGGATCGGGTTTCAGCTCGTCTTCTGGTTTGAATTCATCCATTTAACACCCCACTAAAAGGTTAATGCTTACCACGTTGCAATTAACCTGAAGCTaa                                                        <  1:68927/251‑1 (MQ=255)                                                                                                                                                                                     ggTTCCTCATTGCGCTCTTTTTGCGCACGAGTCGGACGGCGATCGTCAGTGTCATCAAATTCAATATCATCAAAATTGATCTGCGGTTCGCCACGTTCAGTACGCTCAGAACGTTCAGAAGATTGACGAGAACGACCAGTacgaca                                                                                                                                                               >  1:463368/1‑145 (MQ=255)                                                                                                                                                                                       ttCCTCATTGCGCTCTTTTTGAGCACGAGTCGGACGGCGATCGTCAGTGTCATCAAATTCAATATCATCAAAATTGATCTGCGGTTCGCCACGTTCAGTACGCTCAGAACGTTCAGAAGATTGACGAGAACGACCAGTACGACGATCGCTGGGATCGGGTTTCAGCTCGTCTTCTGGTTTGAATTCATCCATTTAACACCCCACTAAAAGGTTAATGCTTACCACGTTGCAATTAACCTGAAGCTAAt                                                       >  2:317432/1‑248 (MQ=255)                                                                                                                                                                                                   ctctTTTTGCGCACGAGTCGGACGGCGATCGTCAGTGTCATCAAATTCAATATCATCAAAATTGATCTGCGGTTCGCCACGTTCAGTACGCTCAGAACGTTCAGAAGATTGACGAGAACGACCAGTACGACGATCGCTGGGATCGGGTTTCa                                                                                                                                           <  1:34214/152‑1 (MQ=255)                                                                                                                                                                                                   ctctTTTTGCGCACGAGTCGGACGGCGATCGTCAGTGTCATCAAATTCAATATCATCAAAATTGATCTGCGGTTCGCCACGTTCAGTACGCTCAGAACGTTCAGAAGATTGACGAGAACGACCAGTACGACGATCGCTGGGATCGGGTTTCa                                                                                                                                           >  2:34214/1‑152 (MQ=255)                                                                                                                                                                                                     ctTTTTGCGCACGAGTCGGACGGCGATCGTCAGTGTCATCAAATTCAATATCATCAAAATTGATCTGCGGTTCGCCACGTTCAGTACGCTCAGAACGTTCAGAAGATTGACGAGAACGACCAGTACGACGATCGCTGGGATCGGGTTTCAGCTCGTCTTCTGGTTTGAATTCATCCATTTAACACCCCACTAAAAGGTTAATGCTTACCACGTTGCAATTAACCTGAAGCTAATAGACCGCTTGATAAg                                        <  2:359130/249‑1 (MQ=255)                                                                                                                                                                                                         ttGCGCACGAGGCGGACGGCGATCGTCAGTGTCATCAAATTCAATATCATCAAAATTGATCTGCGGTTCGCCACGTTCAGTACGCTCAGAACGTTCAGAAGATTGACGAGAACGACCAGTACGACGATCGCTGGGATCGGGTTTCAGCTCGTCTTCTGGTTTGAATTCATCCATTTAACACCCCACTAAAAGGTTAATGCTTACCACGTTGCAATTAACCTGAAGCTAATAGACCGCTTGATAAGCGGcc                                   <  2:340966/250‑1 (MQ=255)                                                                                                                                                                                                                  ggTCGGACGGCGATCGTCAGTGTCATCAAATTCAATATCATCAAAATTGATCTGCGGTTCGCCACGTTCAGTACGCTCAGAACGTTCAGAAGATTGACGAGAACGACCAGTACGACGATCGCTGGGATCGGGTTTCAGCTCGTCTTCTGGTTTGAATTCATCCATTTAACACCCCACTAAAAGGTTAATGCTTACCACGTTGCAATTAACCTGAAGCTAATAGACCGCTTGATAAGCGGCCTGACCTTTCt                         <  2:181212/250‑1 (MQ=255)                                                                                                                                                                                                                      ggACGGCGATCGTCAGTGTCATCAAATTCAATATCATCAAAATTGATCTGCGGTTCGCCACGTTCAGTACGCTCAGAACGTTCAGAAGATTGACGAGAACGACCAGTACGACGATCGCTGGGATCGGGTTTCAGCTCGTCTTCTGGTTTGAATTCATCCATTTAACACCCCACTAAAAGGTTAATGCTTACCACGTTGCAATTAACCTGAAGCTAATAGCCCGCTTGATAAGCGGCCTGACCTTTCttgtt                     >  2:187762/1‑251 (MQ=255)                                                                                                                                                                                                                         cGGCGATCGTCAGTGTCATCAAATTCAATATCATCAAAATTGATCTGCGGTTCGCCACGTTCAGTACGCTCAGAACGTTCAGAAGATTGACGAGAACGACCAGTACGACGATCGCTGGGATCGGGTTTCAGCTCGTCTTCTGGTTTGAATTCATCCATTTAACACCCCACTAAAAGGTTAATGCTTACCACGTTGCAATTAACCTGAAGCTAATAGACCGCTTGATAAGCGGCCTGACCTTTCTTgttgtt                  >  2:113087/1‑251 (MQ=255)                                                                                                                                                                                                                            cGATCGTCAGTGTCATCAAATTCAATATCATCAAAATTGATCTGCGGTTCGCCACGTTCAGTACGCTCAGAACGTTCAGAAGATTGACGAGAACGACCAGTACGACGATCGCTGGGATCGGGTTTCAGCTCGTCTTCTGGTTTGAATTCATCCATTTAACACCCCACTAAAAGGTTAATGCTTACCACGTTGCAATTAACCTGAAGCTAATAGACCGCTTGATAAGCGGCCTGACCTTTCTTGTTGTTACg               <  2:246059/251‑1 (MQ=255)                                                                                                                                                                                                                              aTCGTCAGTGTCTACAAATTCAACATCATCAAAATTGATCGGCGGTGCGCCACTGTCAGTACGCTCAGTACGTTCAGAAGATTGACGAGACCGACCAGTACGACGATCGCTGGGACCGGGTTTCAGATCGTTTTCTGGTTTGAATTCATCCATTTAACACCCCACTAAAAGGTTAATGCTTACCACGTTGCAATTAACCTGAAGCTAATAGACCGCGTGATAAGCGGCCTGACCTTGCTTGTTGTTatg               <  2:325490/249‑3 (MQ=255)                                                                                                                                                                                                                              aTCGTCAGTGTCATCAAGTTCAATATCATCAAAATTGATCTGCGGTTCGCCACGTTCAGTACGCTCAGAACGTTCAGAAGATTGACGAGAACGACCAGTACGACGATCGCTGGGATCGGGTTTCAGCTCGtct                                                                                                                                   <  2:350540/133‑1 (MQ=255)                                                                                                                                                                                                                              aTCGTCAGTGTCATCAAGTTCAATATCATCAAAATTGATCTGCGGTTCGCCACGTTCAGTACGCTCAGAACGTTCAGAAGATTGACGAGAACGACCAGTACGACGATCGCTGGGATCGGGTTTCAGCTCGtct                                                                                                                                   >  1:350540/1‑133 (MQ=255)                                                                                                                                                                                                                              aTCGTCAGTGTCATCAAATTCAATATCATCAAAATTGATCTGCGGCTCGCCACGTTCAGTACGCTCAGAACGTTCAGAAGATTGACGAGAACGACCAGTACGACGATCGCTGGGATCGGGTTTCAGCTCGTCTTCTGGTTTGAATTCATCCATTTAACACCCCACTAAAAGGTTAATGCATACCACGTTGCAATTAACATGAAGCTACTAGACCGCTTGATAAGCGGCCTGACCTTTCTTGTTGTTCCGCt             >  1:283995/1‑251 (MQ=255)                                                                                                                                                                                                                                      tgtCATCAAATTCAATATCATCAAAATTGATCTGCGGTTCGCCACGTTCAGTACGCTCAGAACGTTCAGAAGATTGACGAGAACGACCAGTACGACGATCGCTGGGATCGGGTTTCAGCTCGTCTTCTGGTTTGAATTCATCCATTTa                                                                                                            >  1:196275/1‑148 (MQ=255)                                                                                                                                                                                                                                      tgtCATCAAATTCAATATCATCAAAATTGATCTGCGGTTCGCCACGTTCAGTACGCTCAGAACGTGCAGAAGATTGACGAGAACGACCAGTACGACGATCGCTGGGATCGTGTTTCAGCTCGTCTTCTGGTTCGAATTCATCCATTTa                                                                                                            <  2:196275/148‑1 (MQ=255)                                                                                                                                                                                                                                       gtCATCAAATTCAATATCAACAAAATTGATCTGCGGTTCGCCACGTTCAGTACGCTCAGAACGTTCAGAAGATTGACGAGAACGACCAGTACGACGATCGCTGGGATCGGGTTTCAGCTCGTCTTCTGGTTTGAATTCATCCATTTAACACCCCACTAAAAGGTTAATGCTTACCACGTTGCAATTAACCTGAAGCTAATAGACCGCTTGATAAGCGGCCTGACCTTTCTTGTTGTTACGCTGATTGACaa    <  2:134413/251‑1 (MQ=255)                                                                                                                                                                                                                                         catcaAATTCAATATCATCAAAATTGATCTGCGGTTCGCCACGTTCAGTACGCTCAGAACGTTCAGAAGATTGACGAGAACGACCAGTACGACGATCGCTGGGATCGGGTTTCAGCTCGTCTTCTGGTTTGAATTCATCCATTTAACACCCCACTAAAAGGTTAATGCTTACCACGTTGCAATTAACCTGAAGCTAATAGACCGCTTGATAAGCGGCCTGACCTTTCTTGTTGTTACGCTGATTGACAATc  <  1:415240/251‑1 (MQ=255)                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                     TTTAGCGCAGAACCGATACCGATGATCAACAGCAGTAGAACCAGAATGCCGACGCCCATCATCATATACTGACGAGAAGCGGGTTTACTGGCTGCTTTTTTGCGCTTACGCGGACGACGCTCTACGCGCTCTTCATCCACGGTTTCATCTTCGGATTCGTCAATTTCTTCTTCGATTTCCGGTTCCTCATTGCGCTCTTTTTGCGCACGAGTCGGACGGCGATCGTCAGTGTCATCAAGTTCAATATCATCAAAATTGATCTGCGGTTCGCCACGTTCAGTACGCTCAGAACGTTCAGAAGATTGACGAGAACGACCAGTACGACGATCGCTGGGATCGGGTTTCAGCTCGTCTTCTGGTTTGAATTCATCCATTTAACACCCCACTAAAAGGTTAATGCTTACCACGTTGCAATTAACCTGAAGCTAATAGACCGCTTGATAAGCGGCCTGACCTTTCTTGTTGTTACGCTGATTGACAATC  >  NC_000913/3516933‑3517415

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑