Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,500,129 G→A G74S (GGC→AGC)  frlA → fructoselysine/psicoselysine transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,500,1290GA97.0% 96.8 / ‑3.8 33G74S (GGC→AGC) frlAfructoselysine/psicoselysine transporter
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base A (8/24);  total (9/24)
Fisher's exact test for biased strand distribution p-value = 2.73e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.62e-01

ACGTATTGGACAAGAAAAAGGTATCGCTATGGGAAGCCAGGAACTCCAACGCAAGCTCGGATTTTGGGCCGTTCTTGCAATCGCCGTCGGGACAACCGTCGGCTCCGGTATTTTTGTATCTGTGGGTGAAGTGGCAAAAGCAGCGGGCACGCCGTGGCTTACGGTGCTCGCGTTTGTCATTGGCGGGTTAATTGTGATCCCGCAAATGTGCGTCTATGCGGAACTATCCACCGCTTATCCGGAAAATGGCGCAGATTATGTTTATCTGAAAAATGCCGGAAGCCGACCGCTGGCTTTCCTCTCCGGCTGGGCCAGCTTCTGGGCCAACGATGCGCCGTCATTGTCGATTATGGCGCTGGCGATTGTCAGCAATCTTGGCTTTTTAACGCCTATCGATCCGTTGCTCGGTAAATTTATCGCCGCCGGATTAATTATCGCCTTTATGTTGCTACACCTGCGCTCCGTTGAAGGCGGCGCAGCGTTTCAGACGCT  >  NC_000913/3499882‑3500373
                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                      
aCGTATTGGACAAGAAAAAGGTATCGCTATGGGAAGCCAGGAACTCCAACGCAAGCTCGGATTTTGGGCCGTTCTTGCAATCGCCGTCGGGACAACCGTCGGCTCCGGTATTTTTGTATCTGTGGGTGAAGTGGCAAAAGCAGCGGGCACGCCGTGGCTTACGGTGCTCGCGTTTGTCATTGGCGGGTTAATTGTGATCCCGCAAATGTGCGTCTATGCGGAACTATCCACCGCTTATCCGGAAAATAgcg                                                                                                                                                                                                                                                     <  1:273550/251‑1 (MQ=255)
          cAAGAAAAAGGTATCGCTATGGGAAGCCAGGAACTCCAACGCAAGCTCGGATTTTGGACCGTTCTTGCAATCGCCGTCTGGACAACCGTCGGCTGCGGTATTTTTGTAGCTGTGGGTGAAGTGGCAAAAGCAGCGGGCACGCCTTGGCTTACGGTGCTCTCGTTTGTCATTGGCGGGTTAATTGTGATCCCGCAAATGTGCGTCTATGCGTAACTATCCACCGCTTATCCGGAAAATAGCGCAGATTATGt                                                                                                                                                                                                                                           <  1:52007/251‑1 (MQ=255)
                     gtgcGCTATGGGAAGCCAGGAACTCCACCGCAAGCGCGGATTTTGGGCCGGTCTTGCAATCGCCGTCGGGACAACCTTCGGCTCCGGTATTTTTGTATCTGTGGGTGAAGTGGCAAAAGCAGCGTGCACGCCGTTGGTTACGGTGCGCGCTTTTGTCATTGGCGGGTTAATTGTGATCCCGCAAATGTGCGTCTATGCGGAACTATCCACCGCTTATCCGGAAAATAGCGCAGATTATGTTTATCTGaaaa                                                                                                                                                                                                                                <  1:467749/248‑1 (MQ=255)
                              gggAAGCCAGGAACTCCAACGCAAGCTCGGATTTTGGGCCGTTCTTGCAATCGCCGTCGGGACAACCGTCGGCTCCGGTATTTTTGTATCTGTGGGTGAAGTGGCAAAAGCAGCGGGCACGCCGTGGCTTACGGTGCTCGCGTTTGTCATTGGCGGGTTAATTGTGATCCCGCAAATGTGCGTCTATGCGGAACTATCCACCGCTTATCCGGAAAATAGCGCAGATTATGTTTATCTGAAAAATGCCGGa                                                                                                                                                                                                                       <  1:482361/250‑1 (MQ=255)
                                             ccAACGCAAGCTCGGATTTTGGGCCGTTCTTGCAATCGCCGTCGGGACAACCGTCGGCTCCGGTATTTTTGTATCTGTGGGTGAAGTGGCAAAAGCAGCGGGCACGCCGTGGCTTACGGTGCTCGCGTTTGTCATTGGCGGGTTAATTGTGATCCCGCAAATGTGCGTCTATGCGGAACTATCCACCGCTTATCCGGAAAATAGCGCAGATTATGTTTATCTGAAAAATGCCGGAAGCCGACCGCTGGCtt                                                                                                                                                                                                       <  1:227826/251‑1 (MQ=255)
                                                                      gtgcgtGCAACCGGCGTTGGGACAACCGTCGGGTCGTGTATTGTTTTAGCTGTGGGTGAAGGGGCAAAAGCAGCGGGCACGCCGTGGCTTACGGGGCTCGCGTTTGGCATTGGCGTGTTAATTGAGAACCCGCAAATGTGCGTCTATGCGGAACTATCCACCGCTTATCCAGAAAATAGCGCAGATTATGTGTATCTGAAAAATGCCGGAAGCCGCCCGCTGGTTTTCCTGTCCGGATAGGCCAGCTTCTg                                                                                                                                                                             <  2:189117/246‑1 (MQ=255)
                                                                              aaTCGCCGTCGGGACAACCGTCGGCTCCGGTATTTTTGTATCTGTGGGTGAAGTGGCAAAAGCAGCGGGCACGCCGTGGCTTACGGTGCTCGCGTTTGTCATTGGCGGGTTAATTGTGATCCCGCAAATGTGCGTCTATGCGGAACTATCCACCGCTTATCCGGAAAATAGCGCAGATTATGTTTATCTg                                                                                                                                                                                                                                    <  1:304050/190‑1 (MQ=255)
                                                                                   ccGTCGGGACAACCGTCGGCTCCGGTATTTTTGTATCTGTGGGTGAAGTGGCAAAAGCAGCGGGCACGCCGTGGCTTACGGTGCTCGCGTTTGTCATTGGCGGGTTAATTGTGATCCCGCAAATGTGCGTCTATGCGGAACTATCCACCGCTTATCCGGAAAATAGCGCAGATTATGTTTATCTGAAAAATGCCGGAAGCCGACCGCTGGCTTTCCTCTCCGGCTGGGCCAGCTTCTGGGCCAACGATgcg                                                                                                                                                                <  1:257414/251‑1 (MQ=255)
                                                                                    cGTCGGGACAACCGTCGGCTCCGGTATTTTTGTATCTGTGGGTGAAGTGGCAAAAGCAGCGGGCACGCCGTGGCTTACGGTGCTCGCGTTTGTCATTGGCGGGTTAATTGTGCTCCCGCAAAAGTGCGGCTTTGCGGGACTAACCACCGCCTATTCGGGAAAAAGCGCAGATTATGTTTTTCTGAAAAATGCCTGAAACCGACCGCTTGCTTTCCTCTCCCGCCGCGCCAGCTTCTTGGCCCACCATgcgc                                                                                                                                                               >  1:427058/1‑251 (MQ=255)
                                                                                     ggcGGGACAACCGTCGGCTCCGGTATTTTTGTATCTGTGGGTGAAGTGGCAAAAGCAGCGGGCACGCCGTGGCTTACGGTGCTCGCGTTTGTCATTGGCGGGTTAATTGTGATCCCGCAAATGTGCGTCTATGCGGAACTATCCACCGCTTATCCGGAAAATAGCGCAGATTATGTTTATCTGAAAAATGCCGGAAGCCGACCGCTGGCTTTCCTCTCCGGCTGGGCCAGCTTCTGGGCCAACGATGCGcc                                                                                                                                                              <  1:4686/249‑1 (MQ=255)
                                                                                         ggACAACAGTCGGATCCGGTATTTTTGTATCTGTGGGTGAAGTGGCAAAAGCAGCGGGCACGCCGTGGCTTACGGTGCTCGCGTTTGTCATTGGCGGGTTAATTGTGATCCCGCAAATGTGCGTCTATGCGGAACTATCCACCGCTTATCCGGAAAATAGCGCAGATTATGTTTATCTGAAAAATGCCGGAAGCCGACCGCTGGCTTTCCGCTCCGGCTGGGCCAGCTTCTGGGCCAACGATGCGCCGTCa                                                                                                                                                          <  2:36655/251‑1 (MQ=255)
                                                                                                cGTCGGCTCCGGTATTTTTGTATCTGTGGGTGAAGTGGCAAAAGCAGCGGGCACGCCGTGGCTTACGGTGCTCGCGTTTGTCATTGGCGGGATCATTAGGATTCCCCAAATGTGCGGCTATTCGGGACTATCCACCGACTATTCGGAAAATAACGCAGATTATGTTTTTCTGAAAAAAGCCGCGAACCGACCGCCGGCTTTTCCCTCCGGCCGGGACGGCTTCTTGGGCCACGATGCGCCGGCATTGTCgg                                                                                                                                                   >  1:445913/1‑250 (MQ=255)
                                                                                                    ggCTCCGGTATTTTTGTATCTGTGGGTGAAGTGGCAAAAGCAGCGGGCACGCCGTGGCTTACGGTGCTCGCGTTTGTCATTGGCGGGTTAATTGTGATCCCGCAAATGTGCGTCTATGCGGAACTATCCACCGCTTATCCGGAAAATAGCGCAGATTATGTTTATCTGAAAAATGCCGGAAGCCGACCGCTGGCTTTCCTCTCCGGCTGGGCCAg                                                                                                                                                                                    <  2:387949/215‑1 (MQ=255)
                                                                                                    ggCTCCGGTATTTTTGTATCTGTGGGTGAAGTGGCAAAAGCAGCGGGCACGCCGTGGCTTACGGTGCTCGCGTTTGTCATTGGCGGGGTAATTGTGATCCCGCAAATGTGCGGCTTTTCGGGACTATCCACCGCTTATCCGGGAAATAGCGCAGGTTATGTTTTTCTTAAAAATGCCGGAAGCCGACCGCTGGCTTTCCTCTCCGGCTGGGCCAg                                                                                                                                                                                    >  1:387949/1‑215 (MQ=255)
                                                                                                     gCTCCGGTATTTTTGGTTCTGTGGGTGAAGTGGCAAAAGCAGCGGGCACGCCGTTGCTTACGGTGCTCGCTTTTGTCATTGGCGGGTTAATTGTGATCCCGCAAATGTGCGTCTATGCGGAACTATCCACCGCTTATCCGGAAAATAGCGCAGATTATGTTTATCTGAAAAATGCCGGAAGCCGACCGCTGGCTTTCCTCTCCGGCTGGGCTAGCTTCTGGGCCAACGATGCGCCGTCATTGTCGATTATg                                                                                                                                              <  1:167968/251‑1 (MQ=255)
                                                                                                                 gtgtttCTGTGGGTGAAGTGGCAAAAGCAGCGGGCACGCCGTGGCTTACGGTGCTCGCTTTTGTCATTGGCGGGTTAATTGTGATCCCGCAAATGTGCGTCTATGCGGAACTATCCACCGCTTATCCGGAAAATAGCGCAGATTATGTTTATCTGAAAAATGCCGGAAGCCGACCGCTGGCTTTCCTCTCCGGCTGGGCCAGCTTCTGGGCCAACGATGCGCCGTCATTGTCGATTATGGCGCTGGCGAt                                                                                                                                   <  1:13984/245‑1 (MQ=255)
                                                                                                                                           gcagcGGGCACGCCGTGGCTTACGGTGCTCGCGTTTGTCATTGGCGGGTTAATTGTGATCCCGCAAATGTGCGTCTATGCGGAACTATCCACCGCTTATCCGGAAAATAGCGCAGATTATGTTTATCTGAAAAATGCCGGAAGCCGACCGCTGGCTTTCCTCTCCGGCTGGGCCAGCTTCTGGGCCAACGATGCGCCGTCATTGTCGATTATGGCGCTGGCGATTGTCAGCAATCTTGGCTTTTTAACGcc                                                                                                        <  2:232773/251‑1 (MQ=255)
                                                                                                                                              gcGGGCACGCCTTGGCTTACGGTGCTCGCGTTTGTCATTGGCGGGTTAATTGTGATCCCGCAAATGTGCGTCTATGTGGAACTATCCACCGCTTATCCGGAAAATAGCGCAGATTATGTTTATCTGAAAAATGCCGGAAGCCGACCGCTGGCTTTCCTCTCCGGCTGGGCCAGCTTTTGGGCCAACGATGCGCCGTCATTGTCGATTATGGCGCTGGCGATTGTCAGCAATCTTGGCTTTTTAACGCCTAt                                                                                                     <  2:48401/251‑1 (MQ=255)
                                                                                                                                              gcGGGCACGCCGTGGCTTACGGTGCTCGCGTTTGTCATTGGCGGGTTAATTGTGATCCCGCAAATGTGCGTCTATGCGGAACTATCCACCGCTTATCCGGAAAATAGCGCAGATTATGTTTATCTGAAAAATGCCGGAAGCCGACCGCTGGCTTTCCTCTCCGGCTGGGCCAGCTTCTGGGCCAACGATGCGCCGTCATTGTCGATTATGGCGCTGGCGATTGTCAGCAATCTTGGCTTTTTAACGCCTAt                                                                                                     <  1:240811/251‑1 (MQ=255)
                                                                                                                                                gggCACGCCGTGGCTTACGGTGCTCGCGTTTGTCATTGGCGGGTTAATTGTGATCCCGCAAATGTGCGTCTATGCGGAACTATCCACCGCTTATCCGGAAAATAGCGCAGATTATGTTTATCTGAAAAATGCCGGAAGCCGACCGCTGGCTTTCCTCTCCGGCTGGGCCAGCTTCTGGGCCAACGATGCGCCGTCATTGTCGATTATGGCGCTGGCGATTGTCAGCAATCTTGGCTTTTTAACGCCTATCg                                                                                                   <  1:267934/251‑1 (MQ=255)
                                                                                                                                                       ccGTGGCTTACGGTGCTCGCGTTTGTCATTGGCGGGTTAATTGTGATCCCGCAAATGTGCGTCTATGCGGATCTATCCACCGCTTATCCGGAAAATAGCGCAGATTATGTTTATCTGAAAAATGCCGGAAGCCGACCGCTGGCTTTCCTCTCCGGCTGGGCCAGCTTCTGGGCCAACGATGCGCCGTCATTGTCGATTATGGCGCTGGCGATTGTCAGCAATCTTGGCTTTTTAACGCCTATCGATCCGtt                                                                                            <  1:403252/251‑1 (MQ=255)
                                                                                                                                                           ggCTTACGGTGCTCGCGTTTGTCATTGGCGGGTTAATTGTGATCCCGCAAATGTGCGTCTATGCGGAACTATCCACCGCTTATCCGGAAAATAGCGCAGATTATGTTTATCTGAAAAATGCCGGAAGCCGACCGCTGGCTTTCCTCTCCGGCTGGGCCAGCTTCTGGGCCAACGATGCGCCGTCATTGTCGATTATGGCGCTGGCGATTGTCAGCAATCTTGGCTTTTTAACGCCTATCGATCCGTTGCt                                                                                         <  1:322628/250‑1 (MQ=255)
                                                                                                                                                                    tGCTCGCGTTTGTCATTGGCGGGGTAATTGTGATCCCGCAAATGTGCGTCTATGCGGAACTATCCACCGCCTATTCGGGAAAAAGAGGAGATTATGGTTATTTGAAAAATGCCGGGAGCCGAACGCTGGCTTTCCTCTCCGGCTGGGGCCGCTTCTGGGCCAAAGATGCGCCGTCATTGTCGATTATGGGGGTGGGGGTTGTCAGCAATCTTGGCGTTTTTACGCCTATCGATCCGTTGCTCgggtaattt                                                                               >  1:246735/1‑244 (MQ=255)
                                                                                                                                                                        cgcgTTTGTCATTGGCGGGTTAATTGTGATCCCGCAAATGTGCGTCTAGGCGGAACTATCCACCGCTTATCCGGAAAATAGCGCAGATTATGTTTATCTGAAAAATGCCGGAAGCCGACCGCTGGCTTTCCTCTCCGGCTGGGCCAGCTTCTGGGCCAACGATGCGCCGTCATTGTCGATTATGGCGCTGGCGATTGTCAGCAATCTTGGCTTTTTAACGCCTATCGATCCGTTGCTCGGTAAATTTAt                                                                             <  2:246735/249‑1 (MQ=255)
                                                                                                                                                                           gTTTGTCATTGGCGGGTTAATTGTGATCCCGCAAATGTGCGTCTATGCGGAACTATCCACCGCTTATCCGGAAAATAGCGCAGATTATGTTTATCTGAAAAATGCCGGAAGCCGACCGCTGGCTTTCCTCTCCGGCTGGGCCAGCTTCTGGGCCAACGATGCGCCGTCATTGTCGATTATGGCGCTGGCGATTGTCAGCAATCTTGGCTTTTTAACGCCTATCGATCCGTTGCTCGGTAAATTTATcgccg                                                                        <  1:326320/251‑1 (MQ=255)
                                                                                                                                                                                         ggTTAATTGTGATCCCGCAAATGTGCGTCTATGCGGAACTATCCACCGCTTATCCGGAAAATGGCGCAGATTATGTTTATCTGAAAAATGCCGGAAGCCGACCGCTGGCTTTCCTCTCCGGCTGGGCCAGCTTCTGGGCCAACGATGCGCCGTCATTGTCGATTATGGCGCTGGCGATTGTCAGCAATCTTGGCTTTTTAACGCCTATCGATCCGTTGCTCGGTAAATTTATCGCCGCCGGATTAATTATc                                                          >  1:225519/1‑251 (MQ=255)
                                                                                                                                                                                                   gATCCCGCAAATGTGCGTCTATGCGGAACTATCCACCGCTTATCCGGAAAATAGCGCAGATTATGTTTATCTGAAAAATGCCGGAAGCCGACCGCTGGCTTTCCTCTCCGGCTGGGCCAGCTTCTGGGCCAACGATGCGCCGTCATTGTCGATTATGGCGCTGGCGATTGTCAGCAATCTTGGCTTTTTAACGCCTATCGATCCGTTGCTCGGTAAATTTATCGCCGCCGGATTAATTATCGCCTTTATg                                                 <  1:386460/250‑1 (MQ=255)
                                                                                                                                                                                                                tgCGTCTATGCGGAACTATCCACCGCTTATCCGGAAAATAGCGCAGATTATGTTTATCTGAAAAATGCCGGAAGCCGACCGCTGGCTTTCCTCTCCGGCTGGGCCAGCTTCTGGGCCAACGATGCGCCGTCATTGTCGATTATGGCGCTGGCGATTGTCAGCAATCTTGGCTTTTTAACGCCTATCGATCCGTTGCTCGGTAAATTTATCGCCGCCGGATTAATTATCGCCTTTATGTTGCTACACCTgcg                                   >  2:262198/1‑251 (MQ=255)
                                                                                                                                                                                                                        tGCGGAACTATCCACCGCTTATCCGGAAAATAGCGCAGATTATGTTTATCTGAAAAATGCCGGAAGCCGACCGCTGGCTTTCCTCTCCGGCTGGGCCAGCTTCTGGGCCAACGATGCGCCGTCATTGTCGATTATGGCGCTGGCGCTTGTCAGCAATCTTGGCTTTTTAACGCCTATCGATCCGTTGCTCGGTAAATTTATcg                                                                           >  1:485878/1‑203 (MQ=255)
                                                                                                                                                                                                                        tGCGGAACTATCCACCGCTTATCCGGAAAATAGCGCAGATTATGTTTATCTGAAAAATGCCGGAAGCCGACCGCTGGCTTTCCTCTCCGGCTGGGCCAGCTTCTGGGCCAACGATGCGCCGTCATTGTCGATTATGGCGCTGGCGATTGTCAGCAATCTTGGCTTTTTAACGCCTATCGATCCGTTGCTCGGTAAATTTATcg                                                                           <  2:485878/203‑1 (MQ=255)
                                                                                                                                                                                                                                        gCTTATCCGGAAAATAGCGCAGATTATGTTTATCTGAAAAATGCCGGAAGCCGACCGCTGGCTTTCCTCTCCGGCTGGGCCAGCTTCTGGGCCAACGATGCGCCGTCATTGTCGATTATGGCGCTGGCGATTGTCAGCAATCTTGGCTTTTTAACGCCTATCGATCCGTTGCTCGGTAAATTTATCGCCGCCGGATTAATTATCGCCTTTATGTTGCTACACCTGCGCTCCGTTGAAGGCGGCGCAGCGtt           <  1:137762/251‑1 (MQ=255)
                                                                                                                                                                                                                                                 gAAAATAGCGCAGATTATGTTTATCTGAAAAATGCCGGAAGCCGACCGCTGGCTTTCCTCTCCGGCTGGGCCAGCTTCTGGGCCAACGATGCGCCGTCATTGTCGATTATGGCGCTGGCGATTGTCAGCAATCTTGGCTTTTTAACGCCTATCGATCCGTTGCTCGGTAAATTTATCGCCGCCGGATTAATTATCGACTTTATGTTGCTACACCTGCGCTCCGTTGAAGGCGGCGCCACGTTTCAGACGCt  >  1:266476/1‑251 (MQ=255)
                                                                                                                                                                                                                                                  aaaaTAGCGCAGATTATGTTTATCTGAAAAATGCCGGAAGCCGACCGCTGGCTTTCCTCTCCGGCTGGGCCAGCTTCTGGGCCAACGATGCGCCGTCATTGTCGATTATGGCGCTGGCGATTGTCAGCAATATTGGCTTTTTAACGCCTATCGATCCGTTGCTCGGTAAATTTATCGCCGCCGGATTAATTATCGCCTTTATGTTGCTACACCTGCGCTCCGTTGAAGGCGGCGCAGCGTTTCAGACGCt  >  2:397776/1‑250 (MQ=255)
                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                      
ACGTATTGGACAAGAAAAAGGTATCGCTATGGGAAGCCAGGAACTCCAACGCAAGCTCGGATTTTGGGCCGTTCTTGCAATCGCCGTCGGGACAACCGTCGGCTCCGGTATTTTTGTATCTGTGGGTGAAGTGGCAAAAGCAGCGGGCACGCCGTGGCTTACGGTGCTCGCGTTTGTCATTGGCGGGTTAATTGTGATCCCGCAAATGTGCGTCTATGCGGAACTATCCACCGCTTATCCGGAAAATGGCGCAGATTATGTTTATCTGAAAAATGCCGGAAGCCGACCGCTGGCTTTCCTCTCCGGCTGGGCCAGCTTCTGGGCCAACGATGCGCCGTCATTGTCGATTATGGCGCTGGCGATTGTCAGCAATCTTGGCTTTTTAACGCCTATCGATCCGTTGCTCGGTAAATTTATCGCCGCCGGATTAATTATCGCCTTTATGTTGCTACACCTGCGCTCCGTTGAAGGCGGCGCAGCGTTTCAGACGCT  >  NC_000913/3499882‑3500373

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: