Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,481,131 G→A L11L (CTG→TTG)  kefG ← glutathione‑regulated potassium‑efflux system ancillary protein KefG

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,481,1310GA97.8% 143.8 / NA 46L11L (CTG→TTG) kefGglutathione‑regulated potassium‑efflux system ancillary protein KefG
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (17/28);  minor base C (1/0);  total (18/28)
Fisher's exact test for biased strand distribution p-value = 3.91e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

ACTCTTTCAGTAGCGCCGGGCAGCTATAGGTATAAAGAGGATGCTGAAAGACAATCACCTCGTGCTCGCGCAGTAATGCCTGCTCACGGGGGATATCAATAAAAAAATCGGGATAGTGCGCGTAAAGGTCGTGCACGGTAACATTGCTGAGCTGCGTGGCCGGTTTAAGCAGTACCCGGTTTGCCACCGAGTCCTGAGATTCCGGATGGGCATACAGCAGCAAAACTTTCGCTGGCTGAGACATCATCCCCCTCCCGGTATGGTTTTTGTGTATAGTCGCTGTTTTGGGCTACCATTGCGCCCGGTGCGGCAGCTCGCCCATACATTACATTATCATAATGATAAGTTAACATAGTCTGAACATACGGCACCTTATGATTGTTTTCTCCTCGTTACAAATTCGTCGCGGCGTGCGCGTCCTGCTGGATAATGCCACCGCCACCATCAACCCTGGGC  >  NC_000913/3480915‑3481370
                                                                                                                                                                                                                        |                                                                                                                                                                                                                                               
cctctTGCAGGAGCGCCTGGCAGCTATAGGTATAAAGAGGATGCTGAAAGACAATCTCCTCGTGCTCGCGCAGTAATGCCTGCTCACGGGGGATATCAATAAAAAAATCGGGATAGGGCGCGTAAAGGGCGTGCACGGTAACATTGCTGAGCTGCGGGGCCGGTTTAAGCAGTACCCGGTTTGCCACCGAGTCCTGAGATTCCGGATGGGCATACAACAGCAAAACTTTCGCTGGCTGAGACATCATcccc                                                                                                                                                                                                               <  2:78229/250‑1 (MQ=255)
      tCAGTAGCGCCGGGCAGCTATAGGTATAAAGAGGATGCTGAAAGACAATCACCTCGTGCTCGCGCAGTAATGCCTGCTCACGGGGGATATCAATAAAAAAATCGGGATAGTGCGCGTAAAGGTCGTGCACGGTAACATTGCTGAGCTGCGTGGCCGGTTTAAGCAGTACCCGGTTTGCCACCGAGTCCTGAGATTCCGGATGGGCATACAACAGCAAAACTTTCGCTGGCTGAGACATCATCCCCCTccc                                                                                                                                                                                                          <  2:10144/250‑1 (MQ=255)
                            ggTATAAAGAGGATGCTGAAAGACAATCACCTCGTGCTCGCGCAGTAATGCCTGCTCACGGGGGATATCAATAAAAAAATCGGGATAGTGCGCGTAAAGGTCGTGCACGGTAACATTGCTGAGCTGCGTGGCCGGTTGAAGCAGTACCCGGTTTGCCACCGAGTCCTGAGATTCCGGATGGGCATACAACAGCAAAACTTGCGCTGGCTGAGACAGCATCCCCCTCCCGGTATGGTTTTTGTGTATAGTCg                                                                                                                                                                                   <  2:200741/251‑1 (MQ=255)
                               ataAAGAGGATGCTGAAAGACAATCACTTCGTGCTCGCGCAGTAATGCCTGCTCACGGGGGATATCAATAAAAAAATCGGGATAGTGCGCGTAAATGTCGTGCACGGTAACATTGCTGAGCTGCGTGGCCGGTTTAAGCAGTACCCGGTTTGCCACCGAGTCCTGAGATTCCGGATGGGCATACAACAGCAAAACTTTCGCTTGCTGAGACATCATCCCCCTCCCGGTATGGTTTTTGTGTATAGTCGCTg                                                                                                                                                                                <  2:324816/251‑1 (MQ=255)
                               ataAAGAGGATGCTGAAAGACAATCACCTCGTGCTCGCGCAGTAATGCCTGCTCACGGGGGATATCAATAAAAAAATCGGGATAGTGCGCGTAAAGGTCGTGCACGGTAACATTGCTGAGCTGCGTGGCCGGTTTAAGCAGTACCCGGTTTGCCACCGAGTCCTGAGATTCCGGATGGGCATACAACAGCAAAACTTTCGCTGGCTGAGACATCATCCCCCTCCCGGTATGGTTTTTGTGTATAGTCGCTg                                                                                                                                                                                >  1:192798/1‑251 (MQ=255)
                                 aaaGAGGATGCTGAAAGACAAGCACCTCGTGCTCGCGCAGTAATGCCTGCTCACGGGGGATATCAATAAAAAAATCGGGATAGGGCGCTTAAAGGTCGTTCACGGTAACAGTGCTGAGCTGCGTGGCCGGTTTAAGCAGTACCCGGTTTGCCACCGAGTCCTGAGATTCCGGATGGGCATACAACAGCAAAACTTTCGCTGGCTGAGACATCATCCCCCTCCCTGTATGGTTTTTGTGTATAGTCGCTGtt                                                                                                                                                                              <  2:474391/251‑1 (MQ=255)
                                      ggATGCTGAAAGACAATCACCTCGTGCTCGCGCAGTAATGCCTGCTCACGGGGGATATCAATAAAAAAATCGGGATAGTGCGCGTAAAGGTCGTGCACGGTAACATTGCTGAGCTGCGTGGCCGGTTTAAGCAGTACCCGGTTTGCCACCGAGTCCTGAGATTCCGGATGGGCATACAACAGCAAAACTTTCGCTGGCTGAGACATCATCCCCCTCCCGGTATGGTTTTTGTGTATAGTCGCTGTTTTggg                                                                                                                                                                         >  1:293163/1‑251 (MQ=255)
                                             gAAAGACAATCACCTCGTGCTCGCGCAGTAATGCCTGCTCACGGGGGATATCAATAAAAAAATCGGGATAGTGCGCGTAAAGGTCGTGCACGGTAACATTGCTGAGCTGCGTGGCCGGTTTAAGCAGTACCCGGTTTGCCACCGAGTCCTGAGATTCCGGATGGGCATACAACAGCAAACCTTTCGCTGGCTGAGACATCCTCCCCCTCCCGGTATg                                                                                                                                                                                                    >  2:16078/1‑217 (MQ=255)
                                             gAAAGACAATCACCTCGTGCTCGCGCAGTAATGCCTGCTCACGGGGGATATCAATAAAAAAATCGGGATAGTGCGCGTAAAGGTCGTGCACGGTAACATTGCTGAGCTGCGTGGCCGGTTTAAGCAGTACCCGGTTTGCCACCGAGTCCTGAGATTCCGGATGGGCATACAACAGCAAAACTTTCGCTGGCTGAGACATCATCCCCCTCCCGGTATg                                                                                                                                                                                                    <  1:16078/217‑1 (MQ=255)
                                               aaGACAAGCACCTCGTGGTCGCGCGTTAATGCCTGATCACTGGGGATATCAAGAAAAAAATCGGGATAGTGCGCGTAAAGGTTGTGCACGGTAACATTGCTGGGCTGAGTGGCCTGTTTAAGTAGTACCTGGTTTGCCACCGAGTCCTGAGATTCCGGATGGGCATACTACAGCAAAACTTTCTCTGGCTTAGACATCATCCCCCGCCCGGTATGGTTTTTGGGTATAGTCGCTGTTTCGGGCtgtcattg                                                                                                                                                                <  2:182120/251‑8 (MQ=255)
                                                 gACAATCACCTCGTGCTCGCGCAGTGATGCCTGCTCACGGGGGATATCAATAAAAAAATCGGGATAGTGCGCGTAAAGGTCGTGCACGGTAACATTGCTGAGCTGCGTGGCCGGTTTAAGCAGTACCCGGTTTGCCACCGAGTCCTGAGATTCCGGATGGGCATACAACAGCAAAACTTTCGCTGGCTGAGACATCATCCCCCTCCCGGTATGGTTTTTGTGTATAGTCGCTGTTTTGGGCTACCATTgcg                                                                                                                                                              <  1:66297/251‑1 (MQ=255)
                                                         ccTCGGGCTCGCGCAGTAATGCCTGCTCACGGGGGATATCAATAAAAAAATCGGGATAGTGCGCGTAAAGGTCGTGCACGGTAACATTGCTGAGCTGCGTGGCCGGTTTAAGCAGTACCCGGTTTGCCACCGAGTCCTGAGATTCCGGATGGGCATACAACAGCAAAACTTTCGCTGGCTGAGACATCATCCCCCTCCCGGTATGGTTTTTGTGTATAGTCGCTGTTTTGGGCTACCATTGCGCCCGGTGc                                                                                                                                                      <  2:128605/251‑1 (MQ=255)
                                                               gCTCGCGCAGTAATGCCTGCTCACGGGGGATATCAATAAAAAAATCGGGATAGTGCGCGTAAAGGTCGTGCACGGTAACATTGCTGAGCTGCGTGGCCGGTTTAAGCAGTACCCGGTTTGCCACCGAGTCCTGAGATTCCGGATGGGCATACAACAGCAAAACTTTCGCTGGCTGAGACATCATCCCCCTCCCGGTATGGTTTTTGTGTATAGTCGCTGTTTTGGGCTACCATTGCGCCCGGTGCGGCAGc                                                                                                                                                <  2:191334/251‑1 (MQ=255)
                                                                       cgtaaggccggcgcACGGGGGCTATCAATAAAAAAATCGGGATAGTGCGCGGAAAGGTCGTGCTCGGTAACATTGCTGAGCTGCGTGGCCGTGTTAAGCAGTACCCGGTTTGCCACCGAGTCCTGAGATTCCGGATGGGCAGACAACAGCAAAACTTTCGCTGGCTGAGACATCATCCCCCTCCCGGTATGGTTGTTGTGTATATTCGCTGTTTTGGGCTACCATTGCGCCCGGTGCGGCAGCTCGCCCAt                                                                                                                                        <  2:361174/238‑1 (MQ=255)
                                                                             gCCTGCTCACGGGGGATATCAATAAAAAAATCGGGATAGTGCGCGTAAAGGTCGTGCACGGTAACATTGCTGAGCTGCGTGGCCGGTTTAAGCAGTACCCGGTTTGCCACCGAGTCCTGAGATTCCGGATGGGCATACAACAGCAAAACTTTCGCTGGCTGAGACATCATCCCCCTCCCGGTATGGTTTTTGTGTATAGTCGCTGTTTTGGGCTACCATTGCGCCCGGTGCGGCAGCTCGCCCAtacatt                                                                                                                                   <  1:189338/250‑1 (MQ=255)
                                                                              ccTGCTCACGGGGGATATCAATAAAAAAATCGGGATAGTGCGCGTAAAGGTCGTGCACGGTAACATTGCTGAGCTGCGTGGCCGGTTTAAGCAGTACCCGGTTTGCCACCGAGTCCTGAGATTCCGGATGGGCATACAACAGCAAAACTTTCGCTGGCTGAGACATCATCCCCCTCCCGGTATGGTTTTTGTGTATAGTCGCTGTTTTGGGCTACCATTGCGCCCGGTGCGGCAGCTCGCCCAtacattac                                                                                                                                 <  1:365679/251‑1 (MQ=255)
                                                                              ccTGCTCACGGGGGATATCAATAAAAAAATCGGGATAGTGCGCGTAAAGGTCGTGCACGGTAACATTGCTGAGCTGCGTGGCCGGTTTAAGCAGTACCCGGTTTGCCACCGAGTCCTGAGATTCCGGATGGGCATACAACAGCAAAACTTTCGCTGGCTGAGACATCATCCCCCTCCCGGTATGGTTTTTGTGTATAGTCGCTGTTTTGGGCTACCATTGCGCCCGGTGCGGCAGCTCGCCCAtacattac                                                                                                                                 <  1:413191/251‑1 (MQ=255)
                                                                                 gCTCACGGGGGATATCAATAAAAAAATCGGGATAGTGCGCGTAAAGGTCGTGCACGGTAACATTGCTGAGCTGCGTGGCCGGTTTAAGCAGTACCCGGTTTGCCACCGATTCCTGAGATTCCGGATGGGCATACAACAGCAAAACTTTCGCTGGCTGAGACATCATCCCCCTCCCGGTATGGTTTTTGTGTATAGTCGCTGTTTTGGGCTACCATTGCGCCCGGTGCGGCAGCTCGCCCATacattacatt                                                                                                                              <  1:347480/251‑1 (MQ=255)
                                                                                                             gggATAGTGCGCGTAAAGGTCGTGCACGGTAACATTGCTGAGCTGCGTGGCCGGTTTAAGCAGTACCCGGTTTGCCACCGAGTCCTGAGATTCCGGATGGGCATACAACAGCAAAACTTTCGCTGGCTGAGACATCATCCCCCTCCCGGTATGGTTTTTGTGTATAGTCGCTGTTTTGGGCTACCATTGCGCCCGGTGCGGCAGCTCGCCCATACATTACATTATCATAATGATAAGTTAACATAGTCTg                                                                                                   >  2:438741/1‑250 (MQ=255)
                                                                                                                aTAGTGCGCGTAAAGGTCGTGCACGGTAACATTGCTGAGCTGCGTGGCCGGTTTAAGCAGTACCCGGTTTGCCACCGAGTCCTGAGATTCCGGATGGGCATACAACAGCAAAACTTTCGCTGGCTGAGACATCATCCCCCTCCCGGTATGGTTTTTGTGTATAGTCGCTGTTTTGGGCTACCATTGCGCCCGGTGCGGCAGCTCGCCCATACATTACATTATCATAATGATAAGTTAACATAGTCTGAAc                                                                                                >  1:265263/1‑250 (MQ=255)
                                                                                                                  agggcgcGTAAAGGTCGTGCACGGTAACATTGCTGAGCTGCGTGGCCGGTTTAAGCAGTACCCGGTTTGCCACCGAGTCCTGAGATTCCGGATGGGCATACAACAGCAAAACTTTCGCTGGCTGAGACATCATCCCCCTCCCGGTATGGTTTTTGTGTATAGTCGCTGTTTTGGGCTACCATTGCGCCCGGTGCGGCAGCTCGCCCATACATTACATTATCCTAATGATAAGTTAACATAGTCTGAACAt                                                                                              >  2:204494/4‑250 (MQ=255)
                                                                                                                  aGTGCGCGTAAAGGTCGTGCACGGTAACATTGCTGAGCTGCGTGGCCGGTTTAAGCAGTACCCGGTTTGCCACCGAGTCCTGAGATTCCGGATGGGCATACAACAGCAAAACTTTCGCTGGCTGAGACATCATCCCCCTCCCGGTATGGTTTTTGTGTATAGTCGCTGTTTTGGGCTACCATTGCGCCCGGTGCGGCAGCTCTCCCATACATTACATTATCATAATGATAAGTTAACATAGTCTGAACAt                                                                                              >  2:351554/1‑250 (MQ=255)
                                                                                                                         gTAAAGGTCGTGCACGGTAACATTGCTGAGCTGCGTGGCCGGTTTAAGCAGTACCCGGTTTGCCACCGAGTCCTGAGATTCCGGATGGGCATACAACAGCAAAACTTTCGCTGGCTGAGACATCATCCCCCTCCCGGTATGGTTTTTGTGTATAGTCGCTGTTTTGGGCTACCATTGCGCCCGGTGCGGCAGCTCGCCCATACATTACATTATCATAATGATAAGTTAACATAGTCTGAACATAAGGCAcc                                                                                      >  1:223961/1‑251 (MQ=255)
                                                                                                                           aaaGGTCGTGCACGGTAACATTGCTGAGCTGCGTGGCCGGTTTAAGCAGTACCCGGTTTGCCACCGAGTCCTGAGATTCCGGATGGGCATACAACAGCAAAACTTTCGCTGGCTGAGCCATCATCCCCCTCCCGGTATGGTTTTTGTGTATAGTCGCTGTTTTGGGCTACCATTGCGCCCGGTGCGGCAGCTCGCCCATACATTACATTATCATAATGATAAGTTAACATAATCTGAACAt                                                                                              >  2:165074/1‑241 (MQ=255)
                                                                                                                           aaaGGTCGTGCACGGTAACATTGCTGAGCTGCGTGGCCGGTTTAAGCAGTACCCGGTTTGCCACCGAGTCCTGAGATTCCGGATGGGCATACAACAGCAAAACTTTCGCTGGCTGAGACATCATCCCCCTCCCGGTATGGTTTTTGTGTATAGTCGCTGTTTTGGGCTACCATTGCGCCCGGTGCGGCAGCTCGCCCATACATTACATTATCATAATGATAAGTTAACATAGTCTGAACAt                                                                                              <  1:165074/241‑1 (MQ=255)
                                                                                                                               gTCGTGCACGGTAACATTGCTGAGCTGCGTGGCCGGTTTAAGCAGGACCCGGTTTGCCACCGAGTCCTGAGATTCCGGATGGGCATACAACAGCAAAACTTTCGCTGGCTGAGACATCATCCCCCTCCCGGTATGGTTTTTGTGTATAGTCGCTGTTTTGGGCTACCATTGCGCCCGGTGCGGCAGCTCGCCCATACATTACATTATCATAATGATAAGTTAACATAGTCTGAACATACGGCACCTTATGa                                                                                <  2:471713/251‑1 (MQ=255)
                                                                                                                                  gTGCACGGTAACATTGCTGAGCTGCGTGGCCGGTTTAAGCAGTACCCGGTTTGCCACCGAGTCCTGAGATTCCGGATGGGCATACAACAGCAAAACTTTCGCTGGCTGAGACATCATCCCCCTCCCGGTATGGTTTTTGTGTATAGTCGCTGTTTTGGGCTACCATTGCGCCCGGTGCGGCAGCTCGCCCATACATTACATTATCATAATGATAAGTTAACATAGTCTGAACATACGGCACCTTATGATTg                                                                             <  1:349437/251‑1 (MQ=255)
                                                                                                                                   tGCACGGTAACATTGCTGAGCTGCGTGGCCGGCTTAAGCAGTACCCGGGTTGCCACCGAGTCCTGAGATTCCGGATGGGCATACAACAGCACAACTTTCGCTGGCTGAGACATCATCCCCCTCCCGGTATGGTTTTTGTGTATAGTCGCTGTTTTGGGCTACCATTGCGCCCGGTGCGGCCGCTCGCCCATACCTTACCTTATCATAATGATAAGTTAACATAGTCTGAAAATACGGAACCTTATGATTGt                                                                            >  2:463862/1‑251 (MQ=255)
                                                                                                                                           aaCATTGCTGAGCTGCGTGGCCGGTTTAAGCAGTACCCGGTTTGCCACCGAGTCCTGAGATTCCGGATGGGCATACAACAGCAAAACTTTCGCTGGCTGAGACATCATCCCCCTCCCGGTATGGTTTTTGTGTATAGTCGCTGTTTTGGGCTACCATTGCGCCCGGTGCGGCAGCTCGCCCATACATTACATTATCATAATGATAAGTTAACATAGTCTGAACATACGGCACCTTATGATTGTTTTctcc                                                                     <  1:344948/250‑1 (MQ=255)
                                                                                                                                                 gCTGAGCTGCGTGGCCGGTTTAAGCAGTACCCGGTTTGCCACCGAGTCCTGAGATTCCGGATGGGCATACAACAGCAAAACTTTCGCTGGCTGAGACATCATCCCCCTCCCGGTATGGTTTTTGTGTATAGTCGCTGTTTTGGGCTACCATTGCGCCCGGTGCGGCAGCTCGCCCATACATTACATTATCATAATGATAAGTTAACATAGTCTGAACATACGGCACCTTATGATTGTTTTCTCCTCGTTa                                                               <  1:140464/250‑1 (MQ=255)
                                                                                                                                                        tGCGTGGCCGGATTAAGCAGTACCCGGTTTGCCACCGAGTCCTGAGATTCCGGATGGGCATACAACAGCACAACTTTCGCTGGCTGAGACATCATCCCCCTCCCGGTATGGTTTTTGTGTATAGTCGCTGTTTTGGGCTACCATTGCGCCCGGTGCGGCAGCTCGCCCATACCTTACATTATCATAATGATAAGTTAACATAGTCTGAACATACGGCACCTTATGATTGTTTTCTCCTCGTTACAAATtcg                                                       >  2:364554/1‑251 (MQ=255)
                                                                                                                                                               ccGGTTTAAGCAGTACCCGGTTTGCCACCGAGTCCTGAGATTCCGGATGGGCATACAACAGCAAAACTTTCGCTGGCTGAGACATCATCCCCCTCCCGGTATGGTTTTTGTGTATAGTCGCTGTTTTGGGCTACCATTGCGCCCGGTGCGGCAGCTCGCCCATACATTACATTATCATAATGATAAGTTAACATAGTCTGAACATACGGCACCTTATGATTGTTTTCTCCTCGTTACAAATTCGTCgcggc                                                <  1:417670/251‑1 (MQ=255)
                                                                                                                                                                cGGTTTAAGCAGTACCCGGTTTGCCACCGAGTCCTGAGATTCCGGATGGGCATACAACAGCAAAACTTTCGCTGGCTGAGACATCATCCCCCTCCCGGTATGGTTTTTGTGTATAGTCGCTGTTTTGGGCTACCATTGCGCCCGGTGCGGCAGCTCGCCCAtacatta                                                                                                                                  <  1:422475/168‑1 (MQ=255)
                                                                                                                                                                cGGTTTAAGCAGTACCCGGTTTGCCACCGAGTCCTGAGATTCCGGATGGGCATACAACAGCAAAACTTTCGCTGGCTGAGACATCATCCCCCTCCCGGTATGGTTTTTGTGTATAGTCGCTGTTTTGGGCTACCATTGCGCCCGGTGCGGCAGCTCGCCCAtacatta                                                                                                                                  >  2:422475/1‑168 (MQ=255)
                                                                                                                                                                 ggTTTAAGCAGTACCCGGTTTGCCACCGAGTCCTGAGATTCCGGATGGGCATACAACAGCAAAACTTTCGCTGGCTGAGACATCATCCCCCTCCCGGTATGGTTTTTGTGTATAGTCGCTGTTTTGGGCTACCATTGCGCCCGGTGCGGCAGCTCGCCCATACATTACATTATCATAATGATAAGTTAACATAGTCTGAACATACGGCACCTTATGATTGTTTTCTCCTCGTTACAAATTCGTCGCGGCGt                                              >  1:35725/1‑251 (MQ=255)
                                                                                                                                                                      aaGCAGTACCCGGTTTGCCACCGAGTCCTGAGATTCCGGATGGGCATACAACAGCAAAACTTTCGCTGGCTGAGACATCATCCCCCTCCCGGTATGGTTTTTGTGTATAGTCGCTGTTTTGGGCTACCATTGCGCCCGGTGCGGCAGCTCGCCCATACATTACATTATCATAATGATAAGTTAACATAGTCTGAACATACGGCACCTTATGATTGTTTTCTCCTCGTTACAAATTCGTCGCGGCGTGcgcg                                         <  2:35725/251‑1 (MQ=255)
                                                                                                                                                                       aGCAGTACCCGGTTTGCCACCGAGTCCTGAGATTCCGGATGGGCATACAACAGCAAAACTTTCGCTGGCTGAGACATCATCCCCCTCCCGGTATGGTTTTTGTGTATAGTCGCTGTTTTGGGCTACCATTGCGCCCGGTGCGGCAGCTCGCCCATACATTACATTATCATAATGATAAGTTAACATAGTCTGAACATACGGCACCTTATGATTGGTTTCTCCTCGTTACAAATTCGTCGCGGCGTGCGCGt                                        <  2:272279/251‑1 (MQ=255)
                                                                                                                                                                                    ttGCCACCGAGTCCTGAGATTCCGGATGGGCATACAACAGCAAAACTTTCGCTGGCTGAGACATCATCCCCCTCCCGGTATGGTTTTTGTGTATAGTCGCTGTTTTGGGCTACCATTGCGCCCGGTGCGGCAGCTCGCCCATACATTACATTATCATAATGATAAGTTAACATAGTCTGAACATACGGCACCTTATGATTGTTTTCTCCTCGTTACAAATTCGTCGCGGCGTGCGCGTCCTGCTGGATaa                            <  1:477842/250‑1 (MQ=255)
                                                                                                                                                                                        cACCGAGTCCTGAGATTCCGGATGGGCATACACCAGCAAAACTTCCGCTGGCTGAGACATCATCCCCCTACCGATATGGCTTATGTGTATAGTCGCTGTCTTAGGAAACCATTGCGCCCGGTGCGGCAGATCGCCCATACATTACATTATCATAATGATAAGTTAACATAGTCTGAACATACGGCACCTTATGATTGTTTTCTCCTCGTTACAAATTCGTCGAGGCGAGCGCGTCATGCTGGATAATgcc                        >  1:56207/1‑250 (MQ=255)
                                                                                                                                                                                         aCCGAGTCCTGAGATTCCGGATGGGCATACAACAGCAAAACTTTCGCTGGCTGCGACATCATCCCCCTCCCGGTATGGTTTTTGTGTATAGTCGCTGTTTTGGGCTACCATTGCGCCCGGTGCGGCAGCTCGCCCATACATTACATTATCATAATGATAAGTTAACATAGTCTGAACATACGGCACCTTATGATTGTTTTCTCCTCGTTACAAATTCGTCGCGGCGTGCGCGTCCTGCTGGATAATgccac                      >  1:477337/1‑251 (MQ=255)
                                                                                                                                                                                             gttCCTGAGATTCCGGATGGGCATACAACAGCAAAACTTTCGCTGGCTGAGATATCATCCCCCTCCCGGTATGGTTTTTGTGTATAGTCGCTGTTTTGGGCTACCATTGCGCCCGGTGCGGCAGCTCGCCCATACATTACATTATCATAATGATAAGTTAACATAGTCTGAACATACGGCACCTTATGATTGTTTTCTCCTCGTTACAAATTCGTCGCGGCGTGCGCGTCCTGCTGGATAATgccaccgcc                  <  2:192798/249‑1 (MQ=255)
                                                                                                                                                                                              ggccTGAGATTCCGGATGGGCATACAACAGCAAAACTTTCGCTGGCTGAGACATCATCCCCCTCCCGGTATGGTTTTTGTGTATAGTCGCTGTTTTGGTCTACCATTGCGCCCGGTGCGGCAGCTCGCCCATACATTACATTATCATAATGATAAGTTAACATAGTCTGAACATACGGCACCTTATGATTGTTTTCTCCTCGTTACAAATtcgtcg                                                    <  2:241265/214‑1 (MQ=255)
                                                                                                                                                                                              gTCCTGAGATTCCGGATGGGCATACAACAGCAAAACTTTCGCTGGCTGAGACATCATCCCCCTCCCGGTATGGTTTTTGTGTATAGTCGCTGTTTTGGGCTACCATTGCGCCCGGTGCGGCAGCTCGCCCATACATTACATTATCATAATGATAAGTTAACATAGTCTGAACATACGGCACCTTATGATTGTTTTCTCCTCGTTACAAATtcgtcg                                                    >  1:241265/1‑216 (MQ=255)
                                                                                                                                                                                                   gagaTTCCGGATGGGCATACAACAGCAAAACTTTCGCTGGCTGAGACATCATCCCCCTCCCGGTATGGTTTTTGTGTATAGTCGCTGTTTTGGGCTACCATTGCGCCCGGTGCGGCAGCTCGCCCATACATTACATTATCATAATGATAAGTTAACATAGTCTGAACATACGGCACCTTATGATTGTTTTCTCCTCGTTACAAATtcgtcg                                                    <  2:312481/211‑1 (MQ=255)
                                                                                                                                                                                                   gagaTTCCGGATGGGCATACAACAGCAAAACTTTCGCTGGCTGAGACATCATCCCCCTCCCGGTATGGTTTTTGTGTATAGTCGCTGTTTTGGGCTACCATTGCGCCCGGTGCGGCAGCTCGCCCATACATTACATTATCATAATGATAAGTTAACATAGTCTGAACATACGGCACCTTATGATTGTTTTCTCCTCGTTACAAATtcgtcg                                                    >  1:312481/1‑211 (MQ=255)
                                                                                                                                                                                                              tGGGCATACAACAGCAAAACTTTCGCTGGCTGAGACATCATCCCCCTCCCGGTATGGTTTTTGTGTATAGTCGCTGTTTTGGGCTACCATTGCGCCCGGTGCGGCAGCTCGCCCATACATTACATTATCATAATGATAAGTTAACATAGTCTGAACATACGGCCCCTTATGATTGTTTTCTCCTCGTTCCAAATTCGTCGCGGCGTGCTCGTCCTGCTGGCTAATGCCACCGCCACCATCAACCCTGGGc  >  2:117014/1‑250 (MQ=255)
                                                                                                                                                                                                                        |                                                                                                                                                                                                                                               
ACTCTTTCAGTAGCGCCGGGCAGCTATAGGTATAAAGAGGATGCTGAAAGACAATCACCTCGTGCTCGCGCAGTAATGCCTGCTCACGGGGGATATCAATAAAAAAATCGGGATAGTGCGCGTAAAGGTCGTGCACGGTAACATTGCTGAGCTGCGTGGCCGGTTTAAGCAGTACCCGGTTTGCCACCGAGTCCTGAGATTCCGGATGGGCATACAGCAGCAAAACTTTCGCTGGCTGAGACATCATCCCCCTCCCGGTATGGTTTTTGTGTATAGTCGCTGTTTTGGGCTACCATTGCGCCCGGTGCGGCAGCTCGCCCATACATTACATTATCATAATGATAAGTTAACATAGTCTGAACATACGGCACCTTATGATTGTTTTCTCCTCGTTACAAATTCGTCGCGGCGTGCGCGTCCTGCTGGATAATGCCACCGCCACCATCAACCCTGGGC  >  NC_000913/3480915‑3481370

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑