Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,474,425 T→G K43T (AAA→ACA)  rpsL ← 30S ribosomal subunit protein S12

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,474,4250TG96.9% 90.5 / ‑3.3 32K43T (AAA→ACA) rpsL30S ribosomal subunit protein S12
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base G (15/16);  total (16/16)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.58e-01

TTCACGCCATACTTGGAACGAGCCTGCTTACGGTCTTTAACGCCGGAGCAGTCAAGCGCACCACGTACGGTGTGGTAACGAACACCCGGGAGGTCTTTAACACGACCGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTTTTAGGAGTGGTAGTATATACACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTACCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGTAATAAAACGTCCTCACACAATATGAGGACGCCGAATTTTAGGGCGATGCCGAAAAGGT  >  NC_000913/3474194‑3474650
                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                 
ttCACGCCATACTTGGAACGAGCCTGCTTACGGTCTTTACCGCCGGAGCAGTCAATCGCACCACGTACGGTGTGGTAACGAACACCCGGGAGGTCTTTAACACGACCGCCACGGATCAGGATCACGGAGTGCTCCTGCCGGTTGTGACCATCACCACCTGTGTAGGAAGTCACTTCGCAACCGTTAGTCCGACGAACACGGCATACTTTACGCCGCGCGGAGTTTGGTTTTGTAGGAGTGgtaggatctac                                                                                                                                                                                                                >  2:256821/1‑244 (MQ=255)
        aTACTTGGAACGAGCCTGCTTACGGTCTTTAACGCCGGAGCAGTCAAGCGCACCACGTACGGTGTGGTAACGAACACCCGGGAGGTCTTTAACACGACCGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTTGTAGGAGTGGTAGTATATAACCGAGTAc                                                                                                                                                                                                        >  2:259228/1‑251 (MQ=255)
         ttcgtGGAACGAGCCTTCTTACGGTCTTTAACGCCGGAGCATACAAGCGCACCACGTAGGGGGGGGTAACTAACACCCGGGAGGTATTTAACCCGACCGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTTGTAGGAGTGGTAGTATATACACGAGTAc                                                                                                                                                                                                        <  2:353042/246‑1 (MQ=255)
          aCTTGGACCGAGCCTGCTTACGGTCTTTAACGCCGGAGCAGTCAAGCGCACCACGTACGGTGTGGTAACGAACACCCGGGAGGTCTTTAACACGACCGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTTGTAGGAGTGGTAGTATATACACGAGTACa                                                                                                                                                                                                       <  1:259228/250‑1 (MQ=255)
               gAACGAGCCTGCTTACGGTCTTTAACGCCGGAGCAGTCAAGCGCACCACGTACGGTGTGGTAACGAACACCCGGGAGGTCTTTAACACGACCGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTTGTAGGAGTGGTAGTATATACACGAGTACATACGcc                                                                                                                                                                                                 >  1:115361/1‑251 (MQ=255)
                     gctggCTTACGGTCTTTAACGCCGGAGCAGTCAAGCCCACCCCGTACGGTGTGGTAACGAACACCCGGGAGGTCTTTAACACGTCCGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTTGTAGGAGTGGTAGTATATACACGAGTACATACGCCACGttt                                                                                                                                                                                           <  2:204914/247‑1 (MQ=255)
                             gcggctttaaaCGCCGGAGCAGTCAAGCGCCCCACGGACGGTGTGGTAACGAACACCCGGGAGGTCTTAAACACGACCGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTTGTAGGAGTGGTAGTATATACACGAGTACATACGCCACGTTTTTGCggg                                                                                                                                                                                    <  2:61835/241‑1 (MQ=255)
                                     tAACGCCGGAGCAGTCAAGCGCACCACGTACGGTGTGGTAACGAACACCCGGGAGGTCTTTAACACGACCGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTTGTAGGAGTGGTAGTATATACCCGAGTACATACGCCACGTTTTTGCGGGCATGCTTcc                                                                                                                                                                           >  2:137493/1‑251 (MQ=255)
                                                          caccacGATCGGTGTGGTAACGACCCCCCGGGCGTTCTTTAACACGACCGCCAAGGAACATGATCACGGAGGGCTCCTGCAGGTTGTGACCTTCACCTCCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACTGCATACTTTACGCAGCGCGGAGTTCGGTTTTGTAGGAGTGGTAGTATATACACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCCCGTTGCTTTTc                                                                                                                                                      <  2:90569/251‑1 (MQ=255)
                                                                  aCGGTGTGGTAACGACCCCCCGGGAGGTCTTTAACACGCCCGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCCCCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTTGTAGGAGTGGTAGTATATACACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACt                                                                                                                                                <  1:64035/249‑1 (MQ=255)
                                                                       gtgGTAACGAACACCCGGGAGGTCTTTAACACGACCGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTTGTAGGAGTGGTAGTATATACACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAg                                                                                                                                         <  1:87454/251‑1 (MQ=255)
                                                                                    cccGGGAGGTCTTTAACACGACCGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTTGTAGGAGTGGTAGTATATACACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCg                                                                                                                            >  2:299046/1‑251 (MQ=255)
                                                                                      cGGGAGGTCTTTAACACGACCGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTTGTAGGAGTGGTAGTATATACACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCTCACCTTTGCGAGCACGTGGTTTGCGt                                                                                                                           >  2:140454/1‑250 (MQ=255)
                                                                                                 tAACACGACCGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTTTTAGGAGTGGTAGTATATACACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTACCCGCTGGtt                                                                                                                >  2:236074/1‑250 (MQ=255)
                                                                                                     aacaCCGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTTGTAGGAGTGGTAGTATATACACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTACCAGCTGGTTAACTg                                                                                                           <  1:114611/248‑1 (MQ=255)
                                                                                                      ccaCCGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTTGTAGGAGTGGTAGTATATACACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTACCAGCTGGTTAACTGt                                                                                                          <  2:378402/249‑1 (MQ=255)
                                                                                                       gACCGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTTGTAGGAGTGGTAGTATATACACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTACCAGCTGGTTAACTGtt                                                                                                         >  1:392477/1‑251 (MQ=255)
                                                                                                                       gatcaCGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGCGTTCGGTTTTGTAGGAGTGGTAGTATATACACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTACCAGCTGGTTAACTTTTGCCATTACATAGATcc                                                                                         >  2:35710/1‑251 (MQ=255)
                                                                                                                       gatcaCGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTTGTAGGAGTGGTAGTATATACACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTACCAGCTGGTTAACTGTTGCCATTAAATAGCTcc                                                                                         >  2:228154/1‑251 (MQ=255)
                                                                                                                                 tGCTCCTGCAGGTTGTGACCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTTGTAGGAGTGGTAGTATATACACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTACCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGc                                                                               >  1:306531/1‑251 (MQ=255)
                                                                                                                                    ccctcgaGGTTGTGACGTACTCCACCGATGTAGGAACTCACTTTGAAACCGTTAGTCATATGAATACTGCATACTGTACGCAGCGCGGAGTTCGGTTTTGGAGGAGTTGTAGTATATACACGAGTGCATTCGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGTGCTCGTGGTTTGCGAACCAGCTGGTTAACTGTTGCCTTTAAATAGCTCCTTGTTTTAGCttt                                                                            <  2:197515/245‑1 (MQ=255)
                                                                                                                                          aGGTTGTGACCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTTGTAGGAGTGGTAGTATATACACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTACCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTc                                                                      >  1:477000/1‑251 (MQ=255)
                                                                                                                                                               aTGTAGGAAGGCACGTCGAAACCGTTAGTCAGACGAACAGGGCATACGTTACGCAGCGCGGAGTGCGGTTTTGTAGGAGTTGTAGTATATACACGAGTACATACGCCACGTTTTTGCGGGCATGCTGCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGATCACGTGGTTTGCGTACCAGCTTGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAATCACGTAATAAAACGTc                                                 <  2:47400/251‑1 (MQ=255)
                                                                                                                                                                tggaGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTAAGCAGCGCGGAGTTCGGATTTGTAGGAGTGGTAGTATATACACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAACGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTACCAGCTGGTTAAATGTTCCCATTAAATAGCTCCTGGTTTTCGCTTTTGCTTCGTAAACACGTAAGAAAAcaccc                                                >  2:229268/4‑247 (MQ=255)
                                                                                                                                                                          cACTTCGAAACCGTTAGTCAGACGAACCCGGCATACTTTACGCAGCGCTGTGTTCGGTTTTGTAGGAGTGGTAGTATATACTCGAGTACAGACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTACCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCGTTTGCTTCTTAAACACTTAATAAAACGTCCTCACACAata                                      <  2:277522/251‑1 (MQ=255)
                                                                                                                                                                                     cGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTTGTAGGAGTGGTAGTATATACACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTACCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGTAATAAAACGTCCTCACACAATATGAGGACGCCg                           <  1:431854/251‑1 (MQ=255)
                                                                                                                                                                                                cGAACACGGCATACTTTACGCAGCGCGGCGTTCGGTTTTGTAGGAGTGGTAGTATATACACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTACCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGTAATAAAACGTCCTCACACAATATGAGGACGCCGAATTTTAGGGc                >  2:25263/1‑251 (MQ=255)
                                                                                                                                                                                                      cGGCATACTTTACGCAGCGCGGAGTTCGGTTTTGTAGGAGTGGTAGTATATACCCGAGTACATACGCCACGTTTTTGCTGGCATGCTTCCAGCGCAGGCACGTGGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTACCAGCTGGTTAACTGTTGCCATTAAATAGCCCCTGGTTTTAGCTTTTGCTTCGTAAACACGTAATAAAACGGCCTCACTCAATATGAGGACGCCGTACTTTAGGGCGATGcc          <  2:291814/251‑1 (MQ=255)
                                                                                                                                                                                                       ggCATACTTTACGCAGCGCGGAGTTCGGTTTTGTAGGAGTGGTAGTATATACACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTACCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGTAATAAAACGTCCTCACACAATATGAGGACGCCGAATTTTAGGGCGATGCCg         >  1:375111/1‑251 (MQ=255)
                                                                                                                                                                                                               ttcagccgctgggattgcGGTTTTGTATGAGTGGTAGTATATACTCGAGTTCATTCGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTTCGAGCACGTGGGTTGCGTACCAGCTGGTTAACTGTTGCCATGAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGTAATAAAACGTCCTCACACAATATGAGGACGCCGAATTTTAGGGCGATGCCGAAAAGgt  <  2:272165/233‑1 (MQ=255)
                                                                                                                                                                                                                         cgGAGTTCGGTTTTGTAGGAGTGGTAGTATATACACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTACCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGTAATAAAACGTcc                                                <  2:160025/194‑1 (MQ=255)
                                                                                                                                                                                                                         cgGAGTTCGGTTTTGTAGGAGTGGTAGTATATACACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTACCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGTAATAAAACGTcc                                                >  1:160025/1‑194 (MQ=255)
                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                 
TTCACGCCATACTTGGAACGAGCCTGCTTACGGTCTTTAACGCCGGAGCAGTCAAGCGCACCACGTACGGTGTGGTAACGAACACCCGGGAGGTCTTTAACACGACCGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTTTTAGGAGTGGTAGTATATACACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTACCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGTAATAAAACGTCCTCACACAATATGAGGACGCCGAATTTTAGGGCGATGCCGAAAAGGT  >  NC_000913/3474194‑3474650

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: