Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,934,092	G→A	intergenic (‑404/‑143)	fucA ← / → fucP	L‑fuculose‑phosphate aldolase/L‑fucose:H(+) symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,934,092	0	G	A	100.0%	61.9 / NA	21	intergenic (‑404/‑143)	fucA/fucP	L‑fuculose‑phosphate aldolase/L‑fucose:H(+) symporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (15/6);  total (15/6)

GTTCTCTTTTTATAACCCCATTAAAAATGACCGCTCTTAAAAATATTTATCAAAACGGTCATTTTTCTATTCCTCCAAGCCCGGAATGACCGTTTTCGGCACAAACAATTAATACGGTCATCTGATTTGTGTTTTTTATGATTTATTTTCTGAAACGGGCATGAAATTTCGATTATTAAAGTGATGGTAGTCACATAAAGTCACCTTCTAGCTAATAAGTGTGACCGCCGTCATATTACAGAGCGTTTTTTATTTGAAAATGAATCCATGAGTTCATTTCAGACAGGCAAATATTCACTGATATGAAGCCCGAACTCGCTGGTTTTGCACTTTTGAAAACATAACCGATTACGTGCTTAAGCTTCTGAACCTAAGAGGATGCTATGGGAAACACATCAATACAAACGCAGAGTTACCGTGCGGTAGATAAAGATGCAGGGCAAAGCAGAAGTTACATTATTCCATTCGCGCTGCTGTGCTCACTGTT  >  NC_000913/2933852‑2934338                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                       gTTCTCTTTTTATAACCCCATTAAAAATGACCGCTCTTAAAAATATTTATCAAAACGGTCATTTTTCTATTCCTCCAAGCCCGGAATGACCGTTTTCGGCACAAACAATTAATACGGTCATCTGATTTGTGTTTTTTATGATTTATTTTCTGAAACGGGCATGAAATTTCGATTATTAAAGTGATGGTAGTCACATAAAGTCACCTTCTAGCTAATAAGTGTGACCGCCGTCATATTACAAAGCGtttttt                                                                                                                                                                                                                                              >  1:405058/1‑251 (MQ=255)                                                                        cttccAAGCCCGTAATGACCTTTTTCGGCACAAACAATTAATTCGGTCACCGGTTATGGTTTTTTTATGATTAATTTTCTGAAACGGGCATGAAATTTCGATTATTAAAGTGGTGGTAGTCACATAAAGTCCCCTTCTAGCTAATAAGTGTGACCGCCGTCATATTACAAAGCGTTGTTTATTTGAAAATGAATCCATGAGTTCATTTCAGACAGGCAAATATTCACTGat                                                                                                                                                                                           <  1:293666/229‑1 (MQ=255)                                                                        cctccAAGCCCGGACTGACCGTTTTCGGCACAAACAATTAATACGGTCATCTGATTTGTGTTTTTTATGATTTATTTTCTAAAACGGGCATGAAATTTCGATTATTAAAGTGATGGTAGTCACATAAAGTCACCTTCTAGCTAATAAGAGTGACCGCCGTCATATTACAACACGTTTTTTATTTGAAAATGAATCCATGAGTTCCTTTCAGACAGGCAAATATTCAATGatcgctcggaagcgcgtcgtgt                                                                                                                                                                       >  2:293666/1‑231 (MQ=255)                                                                                                                                         tATGATTTATTTTCTGAAACGGGCATGAAATTTCGATTATTAAAGTGATGGTAGTCACATAAAGTCACCTTCTAGCTAATAAGTGTGACCGCCGTCATATTACAAAGCGTTTTTTATTTGAAAATGAATCCATGAGTTCATTTCAGACAGGCAAATATTCACTGATATGAAGCCCGAACTCGCTGGTTTTGCACTTTTGAAAACATAACCGATTACGTGCTTAAGCTTCTGAACCTAAGAGGATGCTATgg                                                                                                      >  2:379815/1‑251 (MQ=255)                                                                                                                                                     tCTGAAACGGGCATGAAATTTCGATTATTAAAGTGATGGTAGTCACATAAAGTCACCTTCTAGCTAATAAGTGTGACCGCCGTCATATTACAAAGCGTTTTTTATTTGAAAATGAATCCATGAGTTCATTTCAGACAGGCAAATATTCACTGATATGAAGCCCGAACTCGCTGGtt                                                                                                                                                                     >  2:60758/1‑176 (MQ=255)                                                                                                                                                     tCTGAAACGGGCATGAAATTTCGATTATTAAAGTGATGGTAGTCACATAAAGTCACCTTCTAGCTAATAAGTGTGACCGCCGTCATATTACAAAGCGTTTTTTATTTGAAAATGAATCCATGAGTTCATTTCAGACAGGCAAATATTCACTGATATGAAGCCCGAACTCGCTGGtt                                                                                                                                                                     <  1:60758/176‑1 (MQ=255)                                                                                                                                                       tGAAACGGGCCTGAAATTTCGATTATTAAAGTGATGGTAGTCACATAAAGTCACCTTCTAGCTAATAAGTGTGACCGCCGTCATATTACAAAGCGTTTTTTATTTGAAAATGAATCCATGAGTTCATTTCAGACAGGCAAATATTCACTGATATGAAGCCCGAACTCGCTGGTTTTGCACTTTTGACAACATAACCGATTACGTGCTTAAGCTTCTGAACCTAAGAGGATGCTATGGGAAACACATCAAt                                                                                         >  2:427542/1‑250 (MQ=255)                                                                                                                                                                            attattAAAGTGATGGTAGTCACATAAAGTCACCTTCTAGCTAATAAGTGTGACCGCCGTCATATTACAAAGCGTTTTTTATTTGAAAATGAATCCATGAGTTCATTTCAGACAGTCAAATATTCACTGATATGAAGCCCGAACTCGCTGGTTTTGCACTTTTGAAAACATAACCGATTACGTGCTTAAGCTTCTGAACCTAAGAGGATGCTATGGGAAACACATCAATACAAACGCAGAGTTACAGTGCg                                                                   >  1:342650/1‑251 (MQ=255)                                                                                                                                                                                    aGTGATGGTAGTCACATAAAGTCACCTTCTAGCTAATAAGTGTGACCGCCGTCATATTACAAAGCGTTTTTTATTTGAAAATGAATCCATGAGTTCATTTCAGACAGGCAAATATTCACTGATATGAAGCCCGAACTCGCTGGATTTGCACTTTTGAAAACATAACCGATTACGTGCTTAAGCTTCTGAACCTAAGAGGATGCTATGGGAAACACATCAATACAAACGCAGAGTTACCGTGCGGTAGATa                                                            >  1:398860/1‑250 (MQ=255)                                                                                                                                                                                    aGTGATGGTAGGCACGTAAAGTCACCTTCTAGCTAATAAGTGTGACCGCCGTCATATTACAAAGCGTTTTTTATTTGAAAATGAATCCATGAGTTCATTTCAGACAGGCAAATATTCACTGATATGAAGCCCGAACTCGCTGGTTTTGCACTTTTGAAAACATAACCGCTTACGTGCTTAAGCTTCTGAACCTAAGAGGATGCTATGGGAAACACATCAATACAAACGAAGCGTTACCATGCGGTAGATa                                                            >  2:206916/1‑250 (MQ=255)                                                                                                                                                                                             aGTCACATAAAGTCACCTTCTAGCTAATAAGTGTGACCGCCGTCATATTACAAAGCGTTTTTTATTTGAAAATGAATCCATGAGTTCATTTCAGACAGGCAAATATTCACTGATATGAAGCCCGAACTCGCTGGTTTTGCACTTTTGAAAACATAACCGATTACGTGCTTAAGCTTCTGAACCTAAGAGGATGCTATGGGAAACACATCAATACAAACGCAGAGTTACCGTGCGGTAGATAAAGATGCAg                                                   <  1:379815/250‑1 (MQ=255)                                                                                                                                                                                                    tAAAGTCACCTTCTAGCTAATAAGTGTGACCGCCGTCATATTACAAAGCGTTTTTTATTTGAAAATGAATCCATGAGTTCATTTCAGACAGGCAAATATTCACTGATATGAAGCCCGAACTCGCTGGTTTTGCACTTTTGAAAACATAACCGATTACGTGCTTAAGCTTCTGAACCTAAGAGGATGCTATGGGAAACACATCAATACAAACGCAGAGTTACCGTGCGGTAGATAAAGATGCAGGGCAAAGc                                           >  1:400230/1‑251 (MQ=255)                                                                                                                                                                                                     aaaGTCACCTTCTAGCTAATAAGTGTGACCGCCGTCATATTACAAAGCGTTTTTTATTTGAAAATGAATCCATGAGTTCATTTCAGACAGGCAAATATTCACTGATATGAAGCCCGAACTCGCTGGTTTTGCACTTTTGAAAACATAACCGATTACGTGCTTAAGCTTCTGAACCTACGAGGATGCTATGGGAAACACATCAATACAAACGCAGCGTTACCGTGCGGTAGTTAAAGATGCAGGGCAAAGc                                           >  2:433184/1‑250 (MQ=255)                                                                                                                                                                                                          cACCTTCTAGTTAAAAAGTGTGACCGCCGTCATATTACAAAGCGTTTTTTATTTGAAAATGAATCCATGAGTTCATTTCAGACAGGCAAATATTCACTGATATGAAGCCCGAACTCGCTGGTTTTGCACTTTTGAAAACATAACCGATTACGTGCTTAAGCTTCTGAACCTAAGAGGATGCTATGGGAAACACATCAATACAAACGCAGAGTTACCGTg                                                                     <  2:215200/219‑1 (MQ=255)                                                                                                                                                                                                          cACCTTCTAGCTAATAAGTGTGACCGCCGTCATATTACAAAGCGTTTTTTATTTGAAAATGAATCCATGAGTTCATTTCAGACAGGCAAATATTCACTGATATGAAGCCCGAACTCGCTGGTTTTGCACTTTTGAAAACATAACCGATTACGTGCTTAAGCTTCTGAACCTAAGAGGATGCTATGGGAAACACATCAATACAAACGCAGAGTTACCGTg                                                                     >  1:215200/1‑219 (MQ=255)                                                                                                                                                                                                             cTTCTAGCTAATAAGTGTGACCGCCGTCATATTACAAAGCGTTTTTTATTTGAAAATGAATCCATGAGTTCATTTCAGACAGGCAAATATTCACTGATATGAAGCCCGAACTCGCTGGTTTTGCACTTTTGAAAACATAACCGATTACGTGCTTAAGATTCTGAACCTAAGAGGATGCTATGGGAAACACATCAATACAAACGCAGAGTTACCGTGCGGGAGATAAAGATGCAGGGCAAAGCAGACGTTAc                                  >  2:20385/1‑251 (MQ=255)                                                                                                                                                                                                               tCTAGCTAATAAGTGTGACCGCCGTCATATTACAAAGCGTTTTTTATTTGAAAATGAATCCATGAGTTCATTTCAGACAGGCAAATATTCACTGATATGAAGCCCGAACTCGCTGGTTTTGCACGTTTGAAAACATAACCGATTACGTGCTTAAGCTTCTGAACCTAAGAGGATGCTATGTGAAACACATCAATACAAACGCAGAGTTACCGTGCGGTAGATAAAGATGCAGGGCAAAGCAGAAGTTACa                                 <  1:275446/250‑1 (MQ=255)                                                                                                                                                                                                                       ataaGTGTGACCGCCGTCATATTACAAAGCGTTTTTTATTTGAAAATGAATCCATGAGTTCATTTCAGACAGGCAAATATTCACTGATATGAAGCCCGAACTCGCTGGTTTTGCACTTTTGAAAACATAACCGATTACGTGCTTAAGCTTCTGAACCTAAGAGGATGCTATGGGAAACACATCAATACAAACGCAGAGTTACCGTGCGGTAGATAAAGATGCAGGGCAAAGCAGAAGTTACATTATTCCAt                        <  1:427542/251‑1 (MQ=255)                                                                                                                                                                                                                                   gccgTCATATTACAAAGCGTTTTTTATTTGAAAATGAATCCATGAGTTCATTTCAGACAGGCAAATATTCACTGATATGAAGCCCGAACTCGCTGGTTTTGCACTTTTGAAAACATAACCGATTACGTGCTTAAGCTTCTGAACCTAAGAGGATGCTATGGGAAACACATCAATACAAACGCAGAGTTACCGTGCGGTAGATAAAGATGCAGGGCAAAGCAGAAGTTCAATTATTCCATTCGCGctgctgg            >  2:469914/1‑250 (MQ=255)                                                                                                                                                                                                                                     cgTCATATTACAAAGCGTTTTTTATTTGAAAATGAATCCATGAGTTCATTTCAGACAGGCAAATATTCACTGATATGAAGCCCGAACTCGCTGGTTTTGCACTTTTGAAAACATAACCGATTACGTGCTTAAGCTTCTGAACCTAAGAGGATGCTATGGGAAACACATCAATACAAACGCAGAGTTACCGTGCGGTAGATAAAGATGCAGGGCAAAGCAGAAGTTACATTATTCCATTCGCGCTGCTGTGc          >  2:376062/1‑251 (MQ=255)                                                                                                                                                                                                                                             tACAAAGCGTTTTTTATTTGAAAATGAATCCATGAGTTCATTTCAGACAGGCAAATATTCACTGATATGAAGCCCGAACTCGCTGGTTTTGCACTTTTGAAAACATAACCGATTACGTGCTTAAGCTTCTGAACCTAAGAGGATGCTATGGGAAACACATCAATACAAACGCAGAGTTACCGTGCGGTAGATAAAGATGCAGGGCAAAGCAGCAGTTACATTATTCCATTCGCTCTGCTGTGCTCCCTGtt  >  2:130008/1‑251 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                       GTTCTCTTTTTATAACCCCATTAAAAATGACCGCTCTTAAAAATATTTATCAAAACGGTCATTTTTCTATTCCTCCAAGCCCGGAATGACCGTTTTCGGCACAAACAATTAATACGGTCATCTGATTTGTGTTTTTTATGATTTATTTTCTGAAACGGGCATGAAATTTCGATTATTAAAGTGATGGTAGTCACATAAAGTCACCTTCTAGCTAATAAGTGTGACCGCCGTCATATTACAGAGCGTTTTTTATTTGAAAATGAATCCATGAGTTCATTTCAGACAGGCAAATATTCACTGATATGAAGCCCGAACTCGCTGGTTTTGCACTTTTGAAAACATAACCGATTACGTGCTTAAGCTTCTGAACCTAAGAGGATGCTATGGGAAACACATCAATACAAACGCAGAGTTACCGTGCGGTAGATAAAGATGCAGGGCAAAGCAGAAGTTACATTATTCCATTCGCGCTGCTGTGCTCACTGTT  >  NC_000913/2933852‑2934338

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑