Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,774,947	G→A	G544S (GGT→AGT)	yfjW →	uncharacterized protein YfjW

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,774,947	0	G	A	94.4%	48.2 / ‑4.0	18	G544S (GGT→AGT)	yfjW	uncharacterized protein YfjW
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base A (7/10);  total (8/10)
Fisher's exact test for biased strand distribution p-value = 4.44e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.90e-01

AGGTATCTCCCAGTTTAGTATCAACGGGAAACATTTGTCGTTAAGGCCAGAATCGGAGATTATTATTTCTGAAGGGAGTTTGTATGGACTAGTTAATAAAAGCAAAAAAATCAAAATATATGGCACAGCAGATCTTGTTTTTGTTGATAACAAGATAATGAACCTTCGTAAAATAACTTATTTGCAATCTAAGCTAGAAATTTTTGGTTCTTCTATTATGGATATATTAAAGTATATATTTGGTTTAGGTCTGCTAGCAATTTCTATAAAATTCATTCATTCTTACTTTAAGAATGATGTGAATGAAAATTTATTCTTGTAACGCATTTGGCTCCAATTGGAGCCTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGTTTTTGTCGTTACCCATACCTATATTTTCTGTTGTTTAACT  >  NC_000913/2774700‑2775180                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                          aGGTATCTCCCAGTTTAGTATCAACGGGAAACATTTGTCGTTAAGGCCAGAATCGTAGATTAATATTTCTGAAGGGAGTTTGTATGGACTAGTTAATAAAAGCAAAAAAATCAAAATATATGGCACAGCAGATCTTGTTTTTGTTGATAACAAGATAATGAACCTTCGTAAAATAACTTATTTGCAATCTAAGCTAGAAATTTTTGGTTCTTCTATTATGGATATATTAAAGTATATATTTGGTTTAAGTc                                                                                                                                                                                                                                        <  2:384910/251‑1 (MQ=255)                                                  gAATCGGAGATTATTATTTCTGAAGGGAGTTTGTATGGACTAGTTAATAAAAGCAAAAAAATCAAAATATATGGCACAGCAGATCTTGTTTTTGTTGATAACAAGATAATGAACCTTCGTAAAATAACTTATTTGCAATCTAAGCTAGAAATTTTTGGTTCTTCTATTATGGATATATTAAAGTATATATTTGGTTTAAGTCTGCTAGCAATTTCTATAAAATTCATTCATTCTTACttt                                                                                                                                                                                                  <  2:5733/240‑1 (MQ=255)                                                  gAATCGGAGATTATTATTTCTGAAGGGAGTTTGTATGGACTAGTTAATAAAAGCAAAAAAATCAAAATATATGGCACAGCAGATCTTGTTTTTGTTGATAACAAGATAATGAACCTTCGTAAAATAACTTATTTGCAATCTAAGCTAGAAATTTTTGGTTCTTCTATTATGGATATATTAAAGTATATATTTGGTTTAAGTCTGCTAGCAATTTCTATAAAATTCATTCATTCTTACttt                                                                                                                                                                                                  >  1:5733/1‑240 (MQ=255)                                                  gAATCGGAGATTATTATTTCTGAAGGGAGTTTGTATGGACTAGTTAATAAAAGCAAAAAAATCAAAATATATGGCACAGCAGATCTTGTTTTTGTTGATAACAAGATAATGAACCTTCGTAAAATAACTTATTTGCAATCTAAGCTAGAAATTTTTGGTTCTTCTATTATGGATATATTAAAGTATATATTTGGTTTAAGTCTGCTAGCAATTTCTATAAAATTCATTCATTCTTACTTTAAGAATGAtgt                                                                                                                                                                                       <  1:371761/251‑1 (MQ=255)                                                    aTCGGAGATTAATATTTCTGAAGGGAGTTTGTATGGACTAGTTAATAAAAGCAAAAAAATCAAAATATATGGCACAGCAGATCTTGTTTTTGTTGATAACAAGATAATGAACCTTCGTAAAATAACTTATTTGCAATCTAAGCTAGAAATTTTTGGTTCTTCTATTATGGATATATTAAAGTATATATTTGGTTTAAGTCTGCTAGCAATTTCTATAAAATTCATTCATTCTTACTTTAAGAATGATGtga                                                                                                                                                                                     <  2:178730/251‑1 (MQ=255)                                                                                                                      atatGGTACAGCAGATCTTGTTTGTGATTATTACAAGATAATGAACCCTCGTAAAATAACTTATTTGCAATCTAATCTAGAAATTTTTGGTTCTTCTATTATGGATATATTAAAGTATATATTTGGTTTAAGTCTGCTAGCAAGTTCTATAAAATTCTTTCATTCTTACTTTAAGAATGATGTGAATGAAAATTTATTCTTGTAACGCATTTGGCTCCAATTGGAGCCTTTTTATGACTATTTATTaaaa                                                                                                                    <  1:26352/250‑1 (MQ=255)                                                                                                                      atatGGCACAGCAGATCTTGTTTTTGTTGATAACAAGATAATGAACCTTCGTAAAATAACTTATTTGCAATCTAAGCTAGAAATTTTTGTTTCTTCTATTATGGATATATTAAAGTATATATTTGGTTTAAGTCTGCTAGCAATTTCTATAAAATTCATTCATTCTTACTTTAAGAATGATGTGAATGAAAATTTATTCTTGGAACGCATTTGGCTCCAATTGGAGCCTTTTTATGACTATTTATTaaaa                                                                                                                    <  1:26322/250‑1 (MQ=255)                                                                                                                         tGGCACAGCAGATCTTGTTTTTGTTGATAACAAGATAATGAACCTTCGTAAAATAACTTATTTGCAATCTAAGCTAGAAATTTTTGGTTCTTCTATTATGGATATATTAAAGTATATATTTGGTTTAAGTCTGCTAGCAATTTCTATAAAATTCATTCATTCTTACTTTAAGAATGATGTGAATGAAAATTTATTc                                                                                                                                                                       <  1:360266/196‑1 (MQ=255)                                                                                                                         tGGCACAGCAGATCTTGTTTTTGTTGATAACAAGATAATGAACCTTCGTAAAATAACTTATTTGCAATCTAAGCTAGAAATTTTTGGTTCTTCTATTATGGATATATTAAAGTATATATTTGGTTTAAGTCTGCTAGCAATTTCTATAAAATTCATTCATTCTTACTTTAAGAATGATGTGAATGAAAATTTATTc                                                                                                                                                                       >  2:360266/1‑196 (MQ=255)                                                                                                                             acaGCAGATCTTGTTTTTGTTGATAACAAGATAATGAACCTTCGTAAAATAACTTATTTGCATTCTAAGCTAGAAATTTTTGGTTCTTCTATTATGGATATATTAAAGTATATATTTGGTTTAAGTCTGCTAGCAATTTCTATAAAATTCATTCATTCTTACTTTAAGAATGATGTGAATGAAAATTTATTCTTGTAACGCGTTTGGCTCCAATTGGAGCCTTTTTATGACTATTTATTAAAATATCCTa                                                                                                             >  2:107819/1‑250 (MQ=255)                                                                                                                              cagcagATCTTGTTTTTGTTGATAACAAGATAATGAACCTTCGTAAAATAACTTATTTGCAATCTAAGCTAGAAATTTTTGGTTCTTCTATTATGGATATATTAAAGTATATATTTGGTTTAAGTCTGCTAGCAATTTCTATAAAATTCATTCATTCTTACTTTAAGAATGATGTGAATGAAAATTTATTCTTGTAACGCATTTGGCTCCAATTGGAGCCTTTTTATGACTATTTATTAAAATATCCTa                                                                                                             >  1:467515/1‑249 (MQ=255)                                                                                                                                                                              tAACTTATTTGCAATCTAAGCTAGAAATTTTTGGTTCTTCTATTATGGATATATTAAAGTATATATTTGGTTTAAGTCTGCTAGCAATTTCTATAAAATTCATTCATTCTTACTTTAAGAATGATGTGAATGAAAATTTATTCTTGTAACGCATTTGGCTCCAATTGGAGCCTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATAc                                                           >  1:244895/1‑251 (MQ=255)                                                                                                                                                                               aaCTTATTTGCAATCTAAGCTAGAAATTTTTGGTTCTTCTATTATGGATATATTAAAGTATATATTTGGTTTAGGTCTGCTAGCAATTTCTATAAAATTCATTCATTCTTACTTTAAGAATGATGTGAATGAAAATTTATTCTTGTAACGCATTTGGCTCCAATTGGAGCCTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATAcc                                                          >  1:12672/1‑251 (MQ=255)                                                                                                                                                                                   tATTTGCAATCTAAGCTAGAAATTTTTGGTTCTTCTATTATGGATATATTAAAGTATATATTTGGTTTAAGTCTGCTAGCAATTTCTATAAAATTCATTCATTCTTACTTTAAGAATGATGTGAATGAAAATTTATTCTTGTAACGCATTTGGCTCCAATTGGAGCCTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTg                                                        <  1:156366/249‑1 (MQ=255)                                                                                                                                                                                   tATTTGCAATCTAAGCTAGAAATTTTTGGTTCTTCTATTATGGATATATTAAAGTATATATTTGGTTTAAGTCTGCTAGCAATTTCTATAAAATTCATTCATTCTTACTTTAAGAATGATGTGAATGAAAATTTATTCTTGTAACGCATTTGGCTCCAATTGGAGCCTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTg                                                        <  2:244895/249‑1 (MQ=255)                                                                                                                                                                                         cAATCTAAGCTAGAAATTTTTGGTTCTTCTATTATTGATATATTAAAGTATATATTTGGTTGAAGTCTGCTAGCAATTTCTATAAAATTCAGTCATTCTTGCTTTAAGAATGATGTGAATGAAAATTTATTCTTGTAACGCATTTGGCTCCAATTGGAGCCTTGTTATTACTATTGATTAAAATATCCTAAGTGAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAg                                                <  1:432932/251‑1 (MQ=255)                                                                                                                                                                                                                                atatTAAAGTATATATTTGGTTTAAGTCTGCTAGCAATTTCTATAAAATTCATTCATTCTTACTTTAAGAATGATGTGAATGAAAATTTATTCTTGTAACGCATTTGGCTCCAATTGGAGCCTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGTTTTTGTCGTTACCCATACCTATATTTTCtgttg         >  2:125991/1‑251 (MQ=255)                                                                                                                                                                                                                                       aGTATATATTTGGTTTAAGTCTGCTAGCAATTTCTATAAAATTCATTCATTCTTACTTTATGAATGATGTGAATGAAAATTTATTCTTGTAACGCATTTGGCTCCAATTGGAGCCTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGACTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTATAGTGACGTTTTTGTCGTTACCCACACCTATATTTTCTGTTGTTTAACt  >  2:7097/1‑251 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                          AGGTATCTCCCAGTTTAGTATCAACGGGAAACATTTGTCGTTAAGGCCAGAATCGGAGATTATTATTTCTGAAGGGAGTTTGTATGGACTAGTTAATAAAAGCAAAAAAATCAAAATATATGGCACAGCAGATCTTGTTTTTGTTGATAACAAGATAATGAACCTTCGTAAAATAACTTATTTGCAATCTAAGCTAGAAATTTTTGGTTCTTCTATTATGGATATATTAAAGTATATATTTGGTTTAGGTCTGCTAGCAATTTCTATAAAATTCATTCATTCTTACTTTAAGAATGATGTGAATGAAAATTTATTCTTGTAACGCATTTGGCTCCAATTGGAGCCTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGTTTTTGTCGTTACCCATACCTATATTTTCTGTTGTTTAACT  >  NC_000913/2774700‑2775180

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑