Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,684,686	A→C	V274V (GTT→GTG)	glyA ←	serine hydroxymethyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,684,686	0	A	C	100.0%	137.9 / NA	46	V274V (GTT→GTG)	glyA	serine hydroxymethyltransferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (29/17);  total (29/17)

GCTCTTCGGATCGTTCGGTACGCTGTTTTTGTTGACGGTGATGTTAGCACGGCCCAGAGCGGCGTCTGCTTCTTTACCGGTCAGGTTTTTATCAACCAGATCAACCAGGAACAGGTGGTTATCAGTGCCGCCGGAAACCACTTTGTAGCCGCGCTCGAGGAACACTTCTACCATCGCTTTAGCGTTTTTAGCGACCTGCTGCTGGTAAGTTTTGAACTCAGGCTCCATCGCTTCTTTCAGAGCAACCGCTTTACCGGCGATTACGTGCATCAACGGACCGCCCTGACCACCAGGGAAAACGGCAGAGTTCAGTTTTTTGTACAGCTCTTCGCTACCACCTTTCGCCAGGATCAGGCCGCCGCGCGGACCCGCCAGGG‑TTTTGTGAGTGGTGGTAGTAACAACG‑TGAGCATGAGGAACCGGGTTCGGGTAGACGCCAGCAGCAACCAGGCCCGCAACGTGCGCCATATCAACGAACAGGTAAGCAC  >  NC_000913/2684443‑2684927                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                    gCTCTTCGGATCGTTCGGTACGCTGTTTTTGTTGACGGTGATGTTAGCACGGCCCAGAGCGGCGTCTGCTTCTTTACCGGTCAGGTTTTTATCAACCAGATCAACCAGGAACAGGTGGTTATCAGTGCCGCCGGAAACCACTTTGTAGCCGCGCTCGAGGAACACTTCTACCATCGCTTTAGCGTTTTTAGCGACCTGCTGCTGGTAAGTTTTGAACTCATGCTCCATCGCTTCTTTCAGAGCCACCGCtt                                                                                                                                                                                                                                              <  1:136217/251‑1 (MQ=255)     ttCGGATCGTTCGGTACGCTGTTTTTGTTGACGGTGATGTTAGCACGGCCCAGAGCGGCGTCTGCTTCTTTACCGGTCAGGTTTTTATCAACCAGATCAACCAGGAACAGGTGGTTATCAGTGCCGCCGGAAACCACTTTGTAGCCGCGCTCGAGGAACACTTCTACCATCGCTTTAGCGTTTTTAGCGACCTGCTGCTGGTAAGTTTTGAACTCAGGCTCCATCGCTTCTTTCAGAGCCACCGCTTTAcc                                                                                                                                                                                                                                          >  2:388195/1‑251 (MQ=255)       cggaatgTTCGGTACGCTGTTTGTGTTGACGGTGATGTTAGCACGGCCCAGAGCGGCGTCTGCTTCTTTACCGGTCAGGTTTTTATCAACCAGATCAACCAGGAACAGGTGGTTATCAGTGCCGCCGGAAACCACTTTGTAGCCGCGCTCGAGGAACACTTCTACCATCGCTGTAGCGTTTTTAGCGACCTGCTGCTGGTAAGTTTTGAACTCAGGCTCCATCGCTTCTTTCAGAGCCACCGCTTTACCgg                                                                                                                                                                                                                                        <  2:85755/245‑1 (MQ=255)       cGGATCGTTAGGTACGCTGTTTTTGTTGACGGTGATGTTAGCACGGCCCAGAGCGGCGTCTGCTTCTTTACCGGTCAGGTTTTTATCAACCAGATCAACCAGGAACAGGTGGTTATCAGTGCCGCCGAAAACCACTTTGTAGCCGCGCTCGAGGAACACTTCTACCATCGCTTTAGCGTTTTTAGCGACCTGCTGCTGGTAAGTTTTGCACTCAGGCTCCATCGCTTATTTCAGAGCCACCGCTTTACCgc                                                                                                                                                                                                                                        >  1:176257/1‑250 (MQ=255)                cGGTACGCTGTTTTTGTTGACGGTGATGTTAGCACGGCCCAGAGCGGCGTCTGCTTCTTTACCGGTCAGGTTTTTATCAACCAGATCAACCAGGAACAGGTGGTTATCAGTGCCGCCGGAAACCACTTTGTAGCCGCGCTCGAGGAACACTTCTACCATCGCTTTAGCGTTTTTAGCGACCTGCTGCTGGTAAGTTTTGAACTCAGGCTCCATCGCTTCTTTCAGAGCCACCGCTTTACCGGCGATTACGt                                                                                                                                                                                                                               >  1:282953/1‑251 (MQ=255)                         gTTTTTGTTGACGGTGATGTTAGCACGGCCCAGAGCGGCGTCTGCTTCTTTACCGGTCAGGTTTTTATCAACCAGATCAACCAGGAACAGGTGGTTATCAGTGCCGCCGGAAACCACTTTGTAGCCGCGCTCGAGGAACACTTCTACCATCGCTTTAGCGTTTTTAGCGACCTGCTGCTGGTAAGTTTTGAACTCAGGCTCCATCGCTTCTTTCAGAGCCACCGCTTTACCGGCGATTACGTGCATCAACg                                                                                                                                                                                                                      >  1:472119/1‑251 (MQ=255)                             ttgttgACGGTGATGTTAGCACGGCCCAGAGCGGCGTCTGCTTCTTTACCGGTCAGGTTTTTATCAACCAGATCAACCAGGAACAGGTGGTTATCAGTGCCGCCGGAAACCACTTTGTAGCCGCGCTCGAGGAACACTTCTACCATCGCTTTAGCGTTTTTAGCGACCTGCTGCTGGTAAGTTTTGAACTCAGGCTCCATCGCTTCTTTCAGAGCCACCGCTTTACCGGCGATTACGTGCATCAACGGAc                                                                                                                                                                                                                   <  1:401691/250‑1 (MQ=255)                                      gTGATGTTAGCACGGCCCAGAGCGGCGTCTGCTTCTTTACCGGTCAGGTTTTTATCAACCAGATCAACCAGGAACAGGTGGTTATCAGTGCCGCCGGAAACCACTTTGTAGCCGCGCTCGAGGAACACTTCTACCATCGCTTTAGCGTTTTTAGCGACCTGCTGCTGGTAAGTTTTGAACTCAGGCTCCATCGCTTCTTTCAGAGCCACCGCTTTACCGGCGATTACGTGCATCAACGGACCGCCCTGacc                                                                                                                                                                                                         <  1:168587/251‑1 (MQ=255)                                                   ggCCCAGAGCGGCGTCTGCTTCTTTACCGGTCAGGTTTTTATCAACCAGATCAACCAGGAACAGGTGGTTATCAGTGCCGCCGGAAACCACTTTGTAGCCGCGCTCGAGGAACACTTCTACCATCGCTTTAGCGTTTTTAGCGACCTGCTGCTGGTAAGTTTTGAACTCAGGCTCCATCGCTTCTTTCAGAGCCACCGCTTTACCGGCGATTACGTGCATCAACGGACCGCCCTGACCACCAGGGAAAACg                                                                                                                                                                                            >  2:377783/1‑251 (MQ=255)                                                       cAGAGCGGCGTCTGCTTCTTTACCGGTCAGGTTTTTATCAACCAGATCAACCAGGAACAGGTGGTTATCAGTGCCGCCGGAAACCACTTTGTAGCCGCGCTCGAGGAACACTTCTACCATCGCTTTAGCGTTTTTAGCGACCTGCTGCTGGTAAGTTTTGAACTCAGGCTCCATCGCTTCTTTCAGAGCCACCGCTTTACCGGCGATTACGTGCATCAACGGACCGCCCTGACCACCAGGGAAAACGGc                                                                                                                                                                                          >  1:332748/1‑249 (MQ=255)                                                              gcgTCTGCTTCTTTACCGGTCAGGTTTTTATCAACCAGATCAACCAGGAACAGGTGGTTATCAGTGCCGCCGGAAACCACGTTGTAGCCGCGCTCGAGGAACACTTCTACCATCGCTTTAGCGTTTTTAGCGACCTGCTGCTGGTAAGTTTTGAACTCAGGCTCCATCGCTGCTTTCAGAGCCACCGCTTTACCGGCGATTACGTGCATCAACGGACCGCCCTGACCACCAGGGAAAACGGCAGAGTTCAg                                                                                                                                                                                 <  2:176257/251‑1 (MQ=255)                                                                     cttcttTACCGGTCAGGTTTTTATCAACCAGATCAACCAGGAACAGGTGGTTATCAGTGCCGCCGGAAACCACTTTGTAGCCGCGCTCGAGGAACACTTCTACCATCGCTTTAGCGTTTTTAGCGACCTGCTGCTGGTAAGTTTTGAACTCAGGCTCCATCGCTTCTTTCAGAGCCACCGCTTTACCGGCGATTACGTGCATCAACGGACCGCCCTGACCACCAGGGAAAACGGCAGAGTTCAGTTTTTTg                                                                                                                                                                          >  1:396237/1‑251 (MQ=255)                                                                       tcttTACCGGTCAGGTTTTTATCAACCAGATCAACCAGGAACAGGTGGTTATCAGTGCCGCCGGAAACCACTTTGTAGCCGCGCTCGAGGAACACTTCTACCATCGCTTTAGCGTTTTTAGCGACCTGCTGCTGGTAAGTTTTGAACTCAGGCTCCATCGCTTCTTTCAGAGCCACCGCTTTACCGGCGATTACGTGCATCAACGGACCGCCCTGACCACCAGGGAAAACGGCAGAGTTCAGTTTTTTGt                                                                                                                                                                         >  1:234105/1‑250 (MQ=255)                                                                               ggTCAGGTTTTTATCAACCAGATCAACCAGGAACAGGTGGTTATCAGTGCCGCCGGAAACCACTTTGTAGCCGCGCTCGAGGATCACTTCTACCATCGCTTTAGCGTTTTTAGCGACCTGCTGCTGGTAAGTTTTGAACTCAGGCTCCATCGCTTCTTTCAGATCCACCGCTTTACCGGCGATTACGTGCATCAACGGACCGCCCTGACCACCAGGGAAAACGGCAGAGTTCAGTTTTTTGTACAGCTCtt                                                                                                                                                                <  2:282953/251‑1 (MQ=255)                                                                                     gTTTTTATCAACCAGATCAACCAGGAACAGGTGGTTATCAGTGCCGCCGGAAACCACTTTGTAGCCGCGCTCGAGGAACACTTCTACCATCGCTTTAGCGTTTTTAGCGACCTGCTGCTGGTAAGTTTTGAACTCAGGCTCCATCGCTTCTTTCAGAGCCACCGCTTTACCGGCGATTACGTGCATCAACGGACCGCCCTGACCACCAGGGAAAACGGCAGAGTTCAGTTTTTTGTACAGCTCTTCGCTac                                                                                                                                                          <  1:84499/251‑1 (MQ=255)                                                                                                ccagatcaaccagGAACAGGTGGTTATCAGTGCCGCCGGAAACCACTTTGTAGCCGCGCTCGAGGAACACTTCTACCATCGCTTTAGCGTTTTTAGCGACCTGCTGCTGGTAAGTTTTGAACTCAGGCTCCATCGCTTCTTTCAGAGCCACCGCTTTACCGGCGATTACGTGCATCAACGGACCGCCCTGACCACCAGGGAAAACGGCAGGGTTCAGTTTTTTGTACAGCTCTTCGCTACCACCTTTCGcc                                                                                                                                               >  2:381416/1‑251 (MQ=255)                                                                                                  agatcaaccagGAACAGGTGGTTATCAGTGCCGCCGGAAACCACTTTGTAGCCGCGCTCGAGGAACACTTCTACCATCGCTTTAGCGTTTTTAGCGACCTGCTGCTGGTAAGTTTTGAACTCAGGCTCCATCGCTTCTTTCAGAGCCACCGCTTTACCGGCGATTACGTGCATCAACGGACCGCCCTGACCACCAGGGAAAACGGCAGAGTTCAGTTTTTTGTACAGCTCTTCGCTACCACCTTTCGCCAg                                                                                                                                             <  1:403193/251‑1 (MQ=255)                                                                                                   gatcaaccagGAACAGGTGGTTATCAGTGCCGCCGGAAACCACTTTGTAGCCGCGCTCGAGGCACACTTCTACCATCGCTTTAGCGTTTTTAGCGACCTGCTGCTGGTAAGTTTTGAACTCAGGCTCCATCGCTTCTTTCAGAGCCACCGCTTTACCGGCGATTACGTGCATCAACGGACCGCCCTGACCACCAGGGACAACGGCAGAGTTCAGTTTTTTTTACAGCTCTTCGCTACCACCTTTCGCCAgg                                                                                                                                            >  2:293304/1‑251 (MQ=255)                                                                                                                cAGGTGGTTATCAGTGCCGCCGGAACCCACTTTGTAGCCGCGCTCGAGGAACACTTCTACCATCGCTTTAGCGTTTTTAGCGACCTGCTGCTGGTAAGTTTTGAACTCAGGCTCCATCGCTTCTTTCAGAGCCACCGCTTTACCGGCGATTACGTGCATCAACGGACCGCCCTGACCACCAGGGAAAACGGCAGAGTTCAGTTTTTTGTACAGCTCTTCGCTACCACCTTTCGCCCGGATCAGGCcgccgc                                                                                                                               >  1:396670/1‑251 (MQ=255)                                                                                                                                  gccgGAAACCACTTTGTAGCCGCGCTCGAGGAACACTTCTACCATCGCTTTAGCGTTTTTAGCGACCTGCTGCTGGTAAGTTTTGAACTCAGGCTCCATCGCTTCTTTCAGAGCCACCGCTTTACCGGCGATTACGTGCATCAAc                                                                                                                                                                                                                       <  1:363520/145‑1 (MQ=255)                                                                                                                                  gccgGAAACCACTTTGTAGCCGCGCTCGAGGAACACTTCTACCATCGCTTTAGCGTTTTTAGCGACCTGCTGCTGGTAAGTTTTGAACTCAGGCTCCATCGCTTCTTTCAGAGCCACCGCTTTACCGGCGATTACGTGCATCAAc                                                                                                                                                                                                                       >  2:363520/1‑145 (MQ=255)                                                                                                                                       aaaCCACTTTGTAGCCGCGCTCGAGGAACACTTCTACCATCGCTTTAGCGTTTTTAGCGACCTGCTGCTGGTAAGTTTTGAACTCAGGCTCCATCGCTTCTTTCAGAGCCACCGCTTTACCGGCGATTACGTGCATCAACGGACCGCCCTGACCACCAGGGAAAACGGCAGAGTTCAGTTTTTTGTACAGCTCTTCGCTACCACCTTTCCCCCGGATCAGGCCGCCGCGCGGACCCGCCCGGG‑tttgggcg                                                                                                       >  2:114275/1‑246 (MQ=255)                                                                                                                                            aCTTTGTAGCCGCGCTCGAGGAACACTTCTACCATCGCTTTAGCGTTTTTAGCGACCTGCTGCTGGTAAGTTTTGAACTCAGGCTCCATCGCTTCTTTCAGAGCCACCGCTTTACCGGCGATTACGTGCATCAACGGACCGCCCTGACCCCCAGGGAACAAGGCAGAGTTCAGTTTTTTGTACAGCTCTTCGCTACCACCTTTCGCCAGGATCAGGCCGCCGCGCGGACCCGCCAGGGATTTtggattggg                                                                                                   >  2:57805/1‑244 (MQ=255)                                                                                                                                              tttGTAGCCGCGCTCGAGGAACACTTCTACCATCGCTTTAGCGTTTTTAGCGACCTGCTGCTGGTAAGTTTTGAACTCAGGCTCCATCGCTTCTTTCAGAGCCACCGCTTTACCGGCGATTACGTGCATCAACGGACCGCCCTGACCACCAGGGAAACCGGCAGAGTTCAGTTTTTTGTTAAGCTCTTCGCTACCACCTTTCGCCAGGACAAGGCCGCCGCGCGGACCCGCCAGGG‑TTTTATGAGtggtgg                                                                                                >  2:425558/1‑251 (MQ=255)                                                                                                                                                      cgcgCTCGAGGAACACTTCTACCATCGCTTTAGCGTTTTTAGCGACCTGCTGCTGGTAAGTTTTGAACTCAGGCTCCATCGCTTCTTTCAGAGCCACCGCTTTACCGGCGATTACGGGCATCAACGGACCGCCCTGACCACCAGGGAAAACGGCAGAGTTCAGTTTTTTGTACAGCTCTTCGCTACCACCTTTCGCTAGGATCAGGCCGCCGCGCGGACCCGCCAGGG‑TTTTGTGCGTGTGGGTAGTaac                                                                                         >  1:99771/1‑250 (MQ=255)                                                                                                                                                               ggAACACTTCTACCATCGCTTTAGCGTTTTTAGCGACCTGCTGCTGGTAGGTTTTGAACTCAGGCTCCATCGCTTCTTTCAGAGCCACAGCTTTACCGACGATTACGTGCATCAACGGACCGCCCTGACCACCAGGGAAAAAGGCAGGGTTCAGTTTTTTGTACAGCTCTTCGCTACCACCTTTCGCCAGGGTCAGGCCGCCGCGCGGGCCCGCCTGGG‑TTTTGTGGGTGGGTGTAGTAAAAACG‑TGAGcg                                                                              >  2:136792/1‑250 (MQ=255)                                                                                                                                                                 aaCACTTCTACCATCGCTTTAGCGTTTTTAGCGACCTGCTGCTGGTAAGTTTTGAACTCAGGCTCCATCGCTTCTTTCAGAGCCACCGCTTTACCGGCGATTACGTGCATCAACGGACCGCCCTGACCACCAGGGAAAACGGCAGAGTTCAGTTTTTTGTACAGCTCTTCGCTACCACCTTTCGCCAGGATCAGGCCGCCGCGCGGACCCGCCCGGG‑TTTTGTGAGTGGTGGTAGTAACAACG‑TGAGCATg                                                                            >  1:334535/1‑251 (MQ=255)                                                                                                                                                                     cTTCTACCATCGCTTTAGCGTTTTTAGCGACCTGCTGCTGGTAAGTTTTGAACTCAGGCTCCATCGCTTCTTTCAGAGCCACCGCTTTACCGGCGATTACGTGCATCAACGGACCGCCCTGACCACCAGGGAAAACGGCAGAGTTCAGTTTTTTGTACAGCTCTTCGCTACCACCTTTCGCCAGGATCAGGCCGCCGCGCGGACCCGCCAGGG‑GTTTGTGAGTGGTGGTAGTAACAACG‑TGAGCATAAgg                                                                         >  1:375401/1‑250 (MQ=255)                                                                                                                                                                       tCTACAATCGCTTTAGCGTTTTTAGCGACCTGCTGCTGGTAAGTTTTGAACTCAGGCTCCATCGCTTCTTTCAGAGCCACCGCTTTACCGGCGATTACGTGCATCAACGGACCGCCCTGACCACCAGGGAAAACGGCCGAGATCAGTTTTTTGTACAGCTCTTCGCTACCACCTTTCGCCAGGGTCAGGCCGCCACGAGGACCCGCCAGGG‑TTTTGTGAGTGGTGGTAGTAACAACG‑TGAGCCTGAgggac                                                                      >  2:230075/1‑248 (MQ=255)                                                                                                                                                                         tACCATCGCTTTAGCGTTTTTAGCGACCTGCTGCTGGTAAGTTTTGAACTCAGGCTCCATCGCTTCTTTCAGAGCCACCGCTTTACCGGCGATTACGTGCATCAACGGACCGCCCTGACCACCAGGGAAAACGGCAGCGTTCAGTTTTTTGTACAGCTCTTCGCTACCACCTTTCGCCAGGATCAGGCCGCCGCGCGGACCCGCCAGGG‑TTTTGTGAGTGGTGGTAGTAACAACG‑TGAGCATGAGGAACCg                                                                    >  2:111091/1‑251 (MQ=255)                                                                                                                                                                          tccctCGCTTTAGCTTTTTTAGCGACCTGCTGCTGGTATGTTTTGAACTCAGGCTCCATCGCTTCTTTCAGAGCCACCGCTTTCCCGGCGATTACGTGCATCAACGGACCGCCCTGACCACCAGGGAAAACGGCAGAGTTCAGTTTTTTGTACAGCTCTTCGCTACCCCCTTTCGCCAGGATCAGGCCGCCGCGCGGACCCGCCAGGG‑TTTTGTGAGTGGTGGTAGTAACAACG‑TGAGCATGAGGAAc                                                                      <  2:332748/244‑1 (MQ=255)                                                                                                                                                                          aCCATCTCTTTAGCGTTTTTAGCGACCTGCTGCTGGTAAGTTTTGAACTCAGGCTCCAGCGCTTCTGCCAGAGCCACCGCTTTACCGGCGATTACGTGCATCACCGGACCGCCCTGACCACCAGGGAAAACGGCAGAGTTCAGTTTTTTGTACAGCGCTTCGCTACCACCTTTCGCCAGGATCAGGCCGCCGCGCGGACCCGCCAGGG‑TTTTGTGAGTGGTGGTAGTAACAACG‑TGAGCATGAGGAAcc                                                                     <  1:291464/249‑1 (MQ=255)                                                                                                                                                                                    tAGCGTTTTTAGCGACCTGCTGCTGGTAAGTTTTGAACTCAGGCTCCATCGCTTCTTTCAGAGCCACCGCTTTCCCGGCGATTACGTGCATCAACGGACCGCCCTGACCACCAGGGAAAACGGCAGAGTTCAGTTTTTTGTACAGCTCTTCGCTACCACCTTTCGCCAGGATCAGGCCGCCGCGCGGACCCGCCAGGG‑TTTTGTGAGTGGTGGTAGTAACAACG‑TGAGCATGAGGAACCGGGTTCGGGTa                                                          <  1:377783/250‑1 (MQ=255)                                                                                                                                                                                       cGTTTTTAGCGACCGGCTGCGGGTAAGTTTTGAACTCAGGCTCCATCGCTTCTTACAGAGCCACCGCTTTACCGGCAATTGCGTGCATCAACGGACCGCCCTGACCACCAGGGAAAACGGCAGAGTTCAGTTTTTGGTACAGCTCTGCGCTACCACCTGTCGCCAGGATCAGGCGGCCGCGCGGACCCGCCAGGG‑TTTTGTGAGTGGTGGTAGTAACAACG‑TGAGCATGAGGAACCGGGTTCGGGTAGACg                                                      <  1:221085/251‑1 (MQ=255)                                                                                                                                                                                        gTTTTTAGCGACCTGCTGCTGGTAAGTTTTGAACTCAGGCTCCATCGCTTCTTTCAGAGCCACCGCTTTACCGGCGATTACGTGCATCAACGGACCGCCCTGACCACCAGGGAAAACGGCAGAGTTCAGTTTTTTGTACAGCTCTTCGCTACCACCTTTCGCCAGGATCAGGCCGCCGCGCGGACCCGCCAGGG‑TTTTGTGAGTGGTGGTAGTAACAACG‑TGAGCATGAGGAACCGGGTTCGGGTAGACGc                                                     >  2:181508/1‑251 (MQ=255)                                                                                                                                                                                                  aCCTGCTGCTGATAAGTTTTGAACTCAGGCTCCATCGCTTCTTTCAGAGCCACCGCTTTACCGGCGATTACGTGCATCAACTGACCGCCCTGACCACCAGGGAAAACGGCAGAGTTCAGTTTTTTGTACAGCTCTTCGCTACCACCTTTCGCCAGGATCAGGCCGCCGCGCGGACCCGCCAGGG‑GTTTGTGAGTGGTGGTAGTCACAACG‑TGAGCATGAGGAACCGGGTTCGGGTGGACGCCAGCAGCAAc                                           >  2:340786/1‑251 (MQ=255)                                                                                                                                                                                                        tgctgGTAAGTTTTGAACTCAGGCTCCATCGCTTCTTTCAGAGCCACCGCTTTACCGGCGATTACGTGCATCAACGGACCGCCCTGACCACCAGGGAAAACGGCAGAGTTCAGTTTTTTGTACAGCTCTTCGCTACCACCTTTCGCCAGGATCAGGCCGCCGCGCGGACCCGCCAGGG‑TTTTGTGAGTGGTGGTAGTAACAACG‑TGAGCATGAGGAACCGGGTTCGGGTAGACGCCAGCAGCAACCAGGcc                                     >  1:265731/1‑251 (MQ=255)                                                                                                                                                                                                           tgGTAAGTTTTGAACTCAGGCTCCATCGCTTCTTTCAGAGCCACCGCTTTACCGGCGATTACGTGCATCAACGGACCGCCCTGACCACCAGGGAAAACGGCAGAGTTCAGTTTTTTGTACAGCTCTTCGCTACCACCTTTCGCCAGGATCAGGCCGCCGCGCGGACCCGCCAGGG‑TTTTGTGAGTGGTGGGAGTAACAACGGTG‑GCATGAGGAACAGGGTTCGGGTAGACGCCAGCAGCAACCAGACCCGc                                  >  2:131014/1‑251 (MQ=255)                                                                                                                                                                                                                     ggAACTCAGGCTCCATCGCTTCTTACAGAGCCACCGCTTTCCCGGCGATTACGTGCAGCAACGGACCGCCCTGACCACCAGGGAAAACGGCAGAGTGCAGTTTTTTGTACAGCTCTTCGCTACCACCTTTCGCCAGGATCAGGCCGCCGCGCGGACCCGCCAGGG‑TTTTGTGAGTGGTGGTAGTAACAACG‑TGAGCATGAGGAACCGGGTTCGGGTAGACGCCAGCAGCAACCAGGCCCGCAACGTGCGcc                        <  1:53470/250‑1 (MQ=255)                                                                                                                                                                                                                         cTCAGGCTCCATCGCTTCTTTCAGAGCCACCGCTTTACCGGCGATTACGTGCATCAACGGACCGCCCTGACCACCAGGGAAAACGGCAGAGTTCAGTTTTTTGTACAGCTCTTCGCTACCACCTTTCGCCAGGATCAGGCCGCCGCGCGGACCCGCCAGGG‑TTTTGTGAGTGGTGGTAGTAACAACG‑TGAGCATGAGGAACCGGGTTCGGGTAGACGCCAGCAGCAACCAGGCCCGCAACGTGCGCCatat                    >  1:332856/1‑251 (MQ=255)                                                                                                                                                                                                                            aGGCTCCATCGCTTCTTTCAGAGCCACCGCTTTACCGGCGATTACGTGCATCAACGGCCCGCCCTGACCACCAGGGAAAACGGCAGAGTTCAGTTTTTTGTACAGCTCTTCGCTACCACCTTTCGCCAGGATCAGGCCGCCGCGCGGACCCGCCAGGG‑TTTTGTGAGTGGTGGTAGTAACAACG‑TGAGCATGAGGAACCGGGTTCGGGTAGACGCCAGCAGCAACCAGGCCCGCAACGTGCGCCATATCaa                 <  1:181508/251‑1 (MQ=255)                                                                                                                                                                                                                               cTCCATCGCTTCTTTCAGAGCCACCGCTTTACCGGCGATTACGTGCATCAACGGACCGCCCTGACCACCAGGGAAAACGGCAGAGTTCAGTTTTTTGTACAGCTCTTCGCTACCACCTTTCGCCAGGATCAGGCCGCCGCGCGGACCCGCCAGGG‑TTTTGTGAGTGGTGGTAGTAACAACG‑TGAGCATGGGGAACCGGGTTCGGGGAGACGCCCGCAGCAACCAGGCCCGCAAAGTGCGCCATATCAACg               >  2:399177/1‑250 (MQ=255)                                                                                                                                                                                                                                cccATCGCTTCTTTCAGAGCCCCCGCTTTACCGGCGATTACGTGCATCAACGGACCGCCCTGACCACCAGGGAAAACGGCAGAGTTCAGTTTTTTGTACAGCTCTTCGCTACCACCTTTCGCCAGGATCAGGCCGCCGCGCGGACCCGCCAGGG‑TTTTGTGAGTGGTGGTAGTAACAACG‑TGAGCATGAGGAACCGGGTTCGGGTAGACGCCAGCAGCAACCAGGCCCGCAACGTGCGCCATATCAACGaa             <  1:304801/250‑1 (MQ=255)                                                                                                                                                                                                                                    ccGCTTCTTTCAGAGCCCCCGCTTTACCGGCGATTACGTGCATCAACGGACCGCCCTGACCACCAGGGAAAACGGCAGAGTTCAGTTTTTTGTACAGCTCTTCGCTACCACCTTCCGCCAGGATCAGGCCGCCGCGCGGACCCGCCAGGG‑TTTTGTGAGTGGTGGTAGTAACAACG‑TGAGCATGAGGAACCGGGTTCGGGTAGACGCCAGCAGCAACCAGGCCCGCAACGTGCGCCATATCAACGAACAgg         <  1:388195/250‑1 (MQ=255)                                                                                                                                                                                                                                      gCTTCTTTCAGAGCCACCGCTTTACCGGCGATTACGTGCATCAACGGACCGCCCTGACCACCAGGGAAAACGGCAGAGTTCAGTTTTTTGTACAGCTCTTCGCTACCACCTTTCGCCAGGATCAGGCCGCCGCGCGGACCCGCCCGGG‑TTTTGTGAGTGGTGGTAGTAACAACG‑TGAGCATGAGGAACCGGGTTCGGGTAGACGCCAGCAGCAACCAGGCCCGCAACGAGCGCCATATCAACGAACAGGt        >  1:19815/1‑250 (MQ=255)                                                                                                                                                                                                                                           tttCAGAGCCACCGCGTTACCGGCGATTACGTGCATCAACGTACCGCCCTGACCACCAGGGAAAACGGCAGAGTTCAGTTTTTTGTACAGCTCTTCGCTACCCCCTTGCGCCAGGATCAGGCCGCCGCGCGGACCCGCCAGGG‑TTTTGTGAGTGGTGGTAGGAACAACG‑TGAGCCTGGGGAACCGGGATCGGGTAGGCGCAAGCAGCCACCCGGCCCGAAACGTTCGCCATATCAACGACCAGGCAAGCAc  >  2:378205/1‑251 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                    GCTCTTCGGATCGTTCGGTACGCTGTTTTTGTTGACGGTGATGTTAGCACGGCCCAGAGCGGCGTCTGCTTCTTTACCGGTCAGGTTTTTATCAACCAGATCAACCAGGAACAGGTGGTTATCAGTGCCGCCGGAAACCACTTTGTAGCCGCGCTCGAGGAACACTTCTACCATCGCTTTAGCGTTTTTAGCGACCTGCTGCTGGTAAGTTTTGAACTCAGGCTCCATCGCTTCTTTCAGAGCAACCGCTTTACCGGCGATTACGTGCATCAACGGACCGCCCTGACCACCAGGGAAAACGGCAGAGTTCAGTTTTTTGTACAGCTCTTCGCTACCACCTTTCGCCAGGATCAGGCCGCCGCGCGGACCCGCCAGGG‑TTTTGTGAGTGGTGGTAGTAACAACG‑TGAGCATGAGGAACCGGGTTCGGGTAGACGCCAGCAGCAACCAGGCCCGCAACGTGCGCCATATCAACGAACAGGTAAGCAC  >  NC_000913/2684443‑2684927

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑