Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,632,711	G→A	R454C (CGT→TGT)	guaB ←	inosine 5'‑monophosphate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,632,711	0	G	A	97.7%	134.1 / ‑4.5	45	R454C (CGT→TGT)	guaB	inosine 5'‑monophosphate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base A (22/21);  total (24/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.63e-01

GTTGAGTGTACTGAGAACCGAAGTCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACAAACTCCGCTTTAGTACGCAGTTCGTCGATAGTACCACAGCCGGTCAGACCCATACAGGAGCGCAGGCCGCCCATCTGCTGGTGAATGATCTCTTTCAGGCGACCTTTATAGGCTACGCGACCTTCGATACCTTCCGGCACCAGTTTGTCGGCAGCGTTATCGCTCTGGAAATAACGGTCAGAGGAACCTTTGGACATCGCGCCCAGGGAACCCATACCACGGTAAGATTTGTAAGAACGGCCCTGGTAGAGTTCGATTTC  >  NC_000913/2632472‑2632954                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                    gatGAGTGTACTGAGAACAGAAGTCCAGAATGCAGATGCGATGCTTATGATTGTTTTCCGTCATTGCCGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAATAGTATCAGGATCCCAGACGTTAGTTCGGGGACTCTTTAGTAATGATCACGTCTTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACAGCCTCCGCTTTATTACCCAGTtcgtcg                                                                                                                                                                                                                                           >  2:319755/3‑250 (MQ=255)  ttGAGTGTACTGAGAACCGAAGTCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACAAACTCCGCTTTAGTACACAGTtcgtcggt                                                                                                                                                                                                                                         >  1:155983/1‑249 (MQ=255)   tGAGTGGACTGAGAACCGAAGTCCAGAATGAGGATGCGATGCTTAGGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACAAACTCCGCTTTAGTACACAGTTCGTCGAt                                                                                                                                                                                                                                         <  2:266090/250‑1 (MQ=255)     aGTGTACTGAGAACCGAAGTCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACAAACTCCGCTTTAGTACACAGTTCGTCGATAGt                                                                                                                                                                                                                                      <  1:466563/251‑1 (MQ=255)             gagaACCGAAGTCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACAAACTCCGCTTTAGTACACAGTTCGTCGATAGTACcaca                                                                                                                                                                                                                                <  1:74560/249‑1 (MQ=255)                 aCCGAAGTCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACAAACTCCGCTTTAGTACACAGTTCGTCGATAGTACCACAGCCGGt                                                                                                                                                                                                                          <  2:121677/251‑1 (MQ=255)                           aGAATGAGGATGCGATGCTTATGAATGTTTGCAGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACAAACTCCGCTTTAGTACACAGTTCTGCGATAGTACCACAGCCGGTCAGACCGATa                                                                                                                                                                                                                <  1:388724/251‑1 (MQ=255)                                         atgcTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACAAACTCCGCTTTAGTACACAGTTCGTCGATAGTACCACAGCCGGTCAGACCCATACAGGAGCGCAGgcc                                                                                                                                                                                                  >  1:201829/1‑251 (MQ=255)                                                   aTGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGAAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACAAACTCCGCTTTAGTACACAGTTCGACAGTAGTACCCCAGCCGGTCAGACCCATACAGGAGCGCACGCCGCCCATctgc                                                                                                                                                                                        >  2:253072/1‑251 (MQ=255)                                                     gTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACAAACTCCGCTTTAGTACACAGTTCGTCGATAGTACCACAGCCGGTCAGACCCATACAGGAGCGCAGGCCGCCCATctgctg                                                                                                                                                                                      <  1:407821/251‑1 (MQ=255)                                                                                  caaagtaaaCAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAAGCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCCCGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACAAACGCCGCTTTAGTACACAGTTCGTCGATAGTACCACAGCCGGTCAGACCCATACAGGAGCGCAGGCCGCCCATCTGCTGGTGAATGAGCTCTTTCAGGCGTCCTTtat                                                                                                                                                         <  2:281518/245‑1 (MQ=255)                                                                                               aaatataatcgtcccGAAATGAAGCCGGGCGAAGAGAACCAGGAGCCCAGACGGTAGGTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACAAACTCCGCTTTAGTACACAGTTCGTCGATAGTACCACAGCCGGTCAGACCCATACAGGAGCGCAGGCCGCCCATCTGCTGGTGAATGATCTCTTTCAGGCGACCTTTATAGGCTACGCGAcc                                                                                                                                            <  2:140863/239‑1 (MQ=255)                                                                                                     aaaTCGCCCGGCATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGCCTCTTTAGTAATGGTCACGTGGTGAACGTGGCTTTCCTGAATGCCAGCACCGCGGATACGTACAAACTCCGCTTTAGTACACAGTTCGTCGCTAGTACCACAGCAGGTCAGACCCCTACAGGAGAGCAGGCCGCCCATCTGCTGGTGAATGATCACTTTCAGGCGACCTTTATAGGCGACGCGACCTTCGAt                                                                                                                                      >  2:52021/1‑251 (MQ=255)                                                                                                       aTCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACAAACTCCGCTTTAGTACACAGTTCGTCGATAGTACCACAGCCGGTCAGACCCATACAGGAGCGCAGGCCGCCCATCTGCTGGTGAATGATCTCTTTCAGGCGACCTTTATAGGCTACGCGACCTTCGATAc                                                                                                                                    >  1:399612/1‑251 (MQ=255)                                                                                                           cccGACATGAGGTCAGGGGAAGATAATCAGGAGCCCAGTCTGTAGTTCGGGGACGCGTTAGTAATGGGCACGTCGTGAACGTGGCTTTCCGGAATGCCCGCTCCGCTGATACGTACAAACTCCGCTTTAGGACACAGTTCGTCGGTAGTACCACAGCCGGTCAGACCCATACAGGAGCGCAGGCCGCCCATCTGCTGGTGAATGATCTCTTTCAGGCGACCTTTATAGGCTACTCGACCTTCGATTCCTTc                                                                                                                                <  2:455859/251‑1 (MQ=255)                                                                                                                 aTGAAGTGGGGGGAAGAGAATCAGGAGCCCAGACGTTAGGTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACAAACTCCGCTTTAGTACACAGTTCGTCGATAGTACCACAGCCGGTCAGACCCATACAGGAGCGCAGGCCGCCCATCTGCTGGTGAATGATCTCTTTCAGGCGACCTTTATAGGCTACGCGACCTTCGATACCTTCCGGCAc                                                                                                                          <  2:540/251‑1 (MQ=255)                                                                                                                  tGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTCGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACAAACTCCGCTTTAGTACACAGTTCGTCGATAGTACCACAGCCGGTCAGACCCATACAGGCGCGCAGGCCGACCCTCTGCTGGTGAATGATCTCTTTCAGGCGACCTTTATAGGCTACGCGACCTTCGCTACCTTCCGGCAcc                                                                                                                         >  2:471093/1‑251 (MQ=255)                                                                                                                        cGGGCGCAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCAACGCTGATACGTACAAACTCCGCTTTAGTACACAGTTCGTCGATAGTACCACAGCCGGt                                                                                                                                                                                                                          >  1:473081/1‑148 (MQ=255)                                                                                                                        cGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCCCGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACAAACCCCGCTTGAGTACACATTGCGTCGATATTACCACAGCCGGt                                                                                                                                                                                                                          <  2:473081/148‑1 (MQ=255)                                                                                                                        cGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACAAACTCCGCTTTAGTACACAGTTCGTCGATAGTACCACAGCCGGTCAGACCCATACAGGAGCGCAGGCCGCCCATCTGCTGGTGAATGATCTCTTTCAGGCGACCTTTATAGGCTACGCGACCTTCGATACCTTCCg                                                                                                                              >  2:395622/1‑240 (MQ=255)                                                                                                                        cGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACAAACTCCGCTTTAGTACACAGTTCGTCGATAGTACCACAGCCGGTCAGACCCATACAGGAGCGCAGGCCGCCCATCTGCTGGTGAATGATCTCTTTCAGGCGACCTTTATAGGCTACGCGACCTTCGATACCTTCCg                                                                                                                              <  1:395622/240‑1 (MQ=255)                                                                                                                            cGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACAAACTCCGCTTTAGTACACAGTTCGTCGATAGTACCACAGCCGGTCAGACCCATACAGGAGCGCAGGCCGCCCATCTGCTGGTGAATGATCTCTTTCAGGCGACCTTTATAGGCTACGCGACCTTCGATACCTTCCGGCCCCAGTTTGTCgg                                                                                                               >  1:302956/1‑251 (MQ=255)                                                                                                                              aaGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGGCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACAAACTCCGCTTTAGTACACAGTTCGTCGATAGTACCACAGCCGGTCAGACCCATACAGGAGCGCAGGCCGCCCATCTGCTGGTGAATGATCTCTTTCAGGCGACCTTTATAGGCTACGCGACCTTCGATACCTTCCGGCACCAGTTTGTCGGCa                                                                                                             <  1:56653/251‑1 (MQ=255)                                                                                                                                      cAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACAAACTCCGCTTTAGTACACAGTTCGTCGATAGTACCACAGCCGGTCAGACCCATACAGGAGCGCAGGCCGCCCATCTGCTGGTGAATGATCTCTTTCAGGCGACCTTTATAGGCTACGCGACCTTCGATACCTTCCGGCACCAGTTTGTCGGCAGCGTTATc                                                                                                     >  2:206892/1‑251 (MQ=255)                                                                                                                                                 aCGGTAGTTCGGGGAATCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACAAACTCCGCTTTAGTACACAGTACGTCGATAGTACCACAGCCGGTCAGACCCGTACAGGAGCGCAGGCCGCCCATCTGCAGGTGAATGATCCATTTCAGGCGACCTTTATAGGCTAAGCGACCTTCGATACATTCCGGAACCAGGTTGTCGGCAGCGTTATCGCTCTGGCAAt                                                                                          >  1:25236/1‑251 (MQ=255)                                                                                                                                                      aGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACAAACTCCGCTTTAGTACACAGTTCGTCGATAGTACCACAGCCGGTCAGACCCATACAGGAGCGCAGGCCGCCCATCTGCTGGTGAATGATCTCTTTCAGGCGACCTTTATAGGCTACGCGACCTTCGATACCTTCCGGCACCAGTTTGTCGGCAGCGTTATCGCTCTGGAAATAACgg                                                                                     >  1:216120/1‑251 (MQ=255)                                                                                                                                                                ctctTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACAAACTCCGCTTTAGTACACAGTTCGTCGATAGTACCACAGCCGGTCAGACCCATACAGGAGCGCAGGCCGCCCATCTGCTGGTGAATGATCTCTTTCAGGCGACCTTTATAGGCTACGCGACCTTCGATACCTTCCGGCACCAGTTTGTCGGCAGCGTTATCGCTCTGGAAATAACGGTCAGAGGAAc                                                                           >  1:384952/1‑251 (MQ=255)                                                                                                                                                                     tAGTAATGGTCACGTCGTGAACGTGGCTTTCCTTAATGCCCGCACCGCTGATACGTACAAACTCCGCTTTAGTACACAGTTCGTCGATAGTACCACATCCGGTCAGACCCATACAGGAGCGCAGGCCGCCCATCTGCTGGTGAATGATCTCTTTCAGGCGACCTTTATAGGCTACGCGACCTTCGATACCTTCCGGCACCAGATTGTCGGCAGCGTTATCGCTCTGGAAATAACGGTCAGAGGAACCttt                                                                       <  1:205570/250‑1 (MQ=255)                                                                                                                                                                                              gCTTGCCTGAATGCCCGCCCCGCTGATACGTACAAACCCCGCTTTAGTACACAGGTCGTCGATAGTACCACAGCCGGTCAGACCCATACAGGAGCGCAGGCCGCCCATCTGCTGGTGAATGATCTCTTTCAGGCGACCTTTATAGGCTACGCGACCTTCGATACCTTCCGGCACCAGTTTGTCGGCAGCGTTATCGCTCTGGAAATAACGGTCAGAGGAACCTTTGGACATCGCGCCCAGGGAACCCATAc                                             <  2:216120/251‑1 (MQ=255)                                                                                                                                                                                                          gCCCGCACCGCTGATACGTACAAACTCCGCTTTAGTACACAGTTCGTCGATAGTACCACAGCCGGTCAGACCCATACAGGAGCGCAGGCCGCCCATCTGCTGGTGAATGATCTCTTTCAGGCGACCTTTATAGGCTACGCGACCTTCGATACCTTCCGGCACCAGTTTGTCGGCAGCGTTATCGCTCTGGAAATAACGGTCAGAGGAACCTTTGGACATCGCGCCCAGGGAACCCATACCACGGTAAGAtt                                 <  1:402288/251‑1 (MQ=255)                                                                                                                                                                                                           cccGCACCGCTGATACGTACAAACTCCGCTTTAGTACACAGTTCGTCGATAGTACCACAGCCGGTCAGACCCATACAGGAGCGCAGGCCGCCCATCTGCTGGTGAATGATCTCTTTCAGGCGACCTTTATAGGCTACGCGACCTTCGATACCTTCCGGCACCAGTTTGTCGGCAGCGTTATCGCTCTGGAAATAACGGTCAGAGGAACCTTTGGACATCGCGCCCAGGGAACCCATACCACGGTAAGAttt                                >  1:349543/1‑251 (MQ=255)                                                                                                                                                                                                           cccGCACCGCTGATACGTACAAACTCCGCTTTAGTACACAGTTCGTCGATAGTACCACAGCCGGTCAGACCCATACAGGAGCGCAGGCCGCCCATCTGCTGGTGAATGATCTCTTTCAGGCGACCTTTATAGGCTACGCGACCTTCGATACCTTCCGGCACCAGTTTGTCGGCAGCGTTATCGCTCTGGAAATAACGGTCAGAGGAACCTTTGGACATCGCGCCCAGGGAACCCATACCACGGTAAGAttt                                >  1:233926/1‑251 (MQ=255)                                                                                                                                                                                                                aCCGCTGATACGTACAAACTCCGCTTTAGTACACAGTTCGTCGATAGTACCACAGCCGGTCAGACCCATACAGGAGCGCAGGCCGCCCATCTGCTGGTGAATGATCTCTTTc                                                                                                                                                                      >  1:72752/1‑112 (MQ=255)                                                                                                                                                                                                                aCCGCTGATACGTACAAACTCCGCTTTAGTACACAGTTCGTCGATAGTACCACAGCCGGTCAGACCCATACAGGAGCGCAGGCCGCCCATCTGCTGGTGAAGGATCTCTTTc                                                                                                                                                                      <  2:72752/112‑1 (MQ=255)                                                                                                                                                                                                                 ccGCTGATACGTACAAACTCCGCTTTAGTACACAGTTCGTCGATAGTACCACAGCCGGTCAGACCCATACAGGAGCGCAGGCCGCCCATCTGCTGGTGAATGATCTCTTTCAGGCGACCTTTATAGGCTACGCGACCTTCGATACCTTCCGGCACCAGTTTGTCGGCAGCGTTATCGCTCTGGAAATAACGGTCAGAGGAACCTTTGGACATCGCGCCCAGGGAACCCATACCACGGTAAGATTTGTa                             >  1:124108/1‑248 (MQ=255)                                                                                                                                                                                                                 ccGCTGATACGTACAAACTCCGCTTTAGTACACAGTTCGTCGATAGTACCACAGCCGGTCAGACCCATACAGGAGCGCAGGCCGCCCATCTGCTGGTGAATGATCTCTTTCAGGCGACCTTTATAGGCTACGCGACCTTCGATACCTTCCGGCACCAGTTTGTCGGCAGCGTTATCGCTCTGGAAATAACGGTCAGAGGAACCTTTGGACATCGCGCCCAGGGAACCCATACCACGGTAAGATTTGTa                             >  1:467126/1‑248 (MQ=255)                                                                                                                                                                                                                    cTGATACGTACAAACTCCGCTTTAGTACACAGTTCGTCGATAGTACCACAGCCGGTCAGACCCATACAGGAGCGCAGGCCGCCCATCTGCTGGTGAATGATCTCTTTCAGGCGACCTTTATAGGCTACGCGACCTTCGATACCTTCCGGCACCAGTTTGTCGGCAGCGTTATCGCTCTGGAAATAACGGTCAGAGGAACCTTTGGACATCGCGCCCAGGGAACCCATACCACGGTAAGATTTGTAAGAACg                       >  1:150180/1‑251 (MQ=255)                                                                                                                                                                                                                      gATACGTACAAACTCCGCTTTAGTACACAGTTCGTCGATAGTACCACAGCCGGTCAGACCCATACAGGAGCGCAGGCCGCCCATCTGCTGGTGAATGATCTCTTTCAGGCGACCTTTATAGGCTACGCGACCTTCGATACCTTCCGGCACCAGTTTGTCGGCAGCGTTATCGCTCTGGAAATAACGGTCAGAGGAACCTTTGGACATCGCGCCCAGGGAACCCATACCACGGTAAGATTTGTAAGAACg                       <  1:471093/249‑1 (MQ=255)                                                                                                                                                                                                                           gtccAAACTCCCCTTTAGTACACAGTTCTCCGATAGTACCACAGCCGGTCAGACCCATACAGGAGCGCAGGCCGCCCATCTGCTGGTGAATGATCTCTTTCAGGCGACCTTTATAGGCTACGCGACCTTCGATACCTTCCGGCACCAGTTTGTCGGCAGCGTTATCGCTCTGGAAATAACGGTCAGAGGAACCTTTTGACATCGCGCCCAGGGAACCCATACCACGGTAAGATGTGTAAGAACGGCCCTgg                <  2:457472/248‑1 (MQ=255)                                                                                                                                                                                                                           gTACAAACTCCGCTTTAGTACACAGTTCGTCGATAGTACCACAGCCGGTCAGACCCATACAGGAGCGCAGGCCGCCCATCTGCTGGTGAATGATCTCTTTCAGGCGACCTTTATAGGCTACGCGACCTTCGATACCTTCCGGCACCAGTTTGTCGGCAGCGTTATCGCTCTGGAAATAACGGTCAGCGGAACCTTTGGACATCGCGCCCAGGGAACCCATACCACGGTAAGATTTGTAAGAACgg                      >  2:76680/1‑245 (MQ=255)                                                                                                                                                                                                                           gTACAAACTCCGCTTTAGTACACAGTTCGTCGATAGTACCACAGCCGGTCAGACCCATACAGGAGCGCAGGCCGCCCATCTGCTGGTGAATGATCTCTTTCAGGCGACCTTTATAGGCTACGCGACCTTCGATACCTTCCGGCACCAGTTTGTCGGCAGCGTTATCGCTCTGGAAATAACGGTCAGAGGAACCTTTGGACATCGCGCCCAGGGAACCCATACCACGGTAAGATTTGTAAGAACgg                      <  1:76680/245‑1 (MQ=255)                                                                                                                                                                                                                              cAAACTCCGCTTTAGTACGCAGTTCGTCGATAGTACCACAGCCGGTCAGACCCATACAGGAGCGCAGGCCGCCCATCTGCTGGTGAATGATCTCTTTCAGGCGACCTTTATAGGCTACGCGACCTTCGATACCTTCCGGCACCAGTTTGTCGGCAGCGTTATCGCTCTGGAAATAACGGTCAGAGGAACCTTTGGACATCGCGCCCAGGGAACCCATACCACGATAAGATTTGTAAGAACGGCCCTGGtgg             >  2:322800/1‑249 (MQ=255)                                                                                                                                                                                                                                  cTCCGCTTTAGTAAACAGTTCGTCGATAGTACCACCGCCGGTCAGACACATACAGGAGCGCAGGCCGCCCATCTGCTGGTGACTGATCTCTTTCAGGCGACCTTTATCGGCTACGCGCCCTTCGATAACTTCAAGCACCAGTTTGTCGTCAGCGTTATCGCTCTGGAAATAACGGTCAGAGGAACCTTTGGACATAGCGCCCCGGGCACCCATACCACGGTAAGATTTGTAAGCCCGGCCATGGTCGAGtt         >  2:241660/1‑251 (MQ=255)                                                                                                                                                                                                                                     cGCTTTAGTACACAGTTCGTCGATAGTACCACAGCCGGTCAGACCCATACAGGAGCGCAGGCCGCCCATCTGCTGGTGAATGATCTCTTTCAGGCGACCTTTATAGGCTACGCGACCTTCGATACCTTCCGGCACCAGTTTGTCGGCAGCGTTATCGCTCTGGAAATAACGGTCAGAGGAACCTTTGGACATCGCGCCCAGGGAACCCATACCACGGTAAGATTTGTAAGAACGGCCCTGGAAGAGTTCg       >  2:342783/1‑250 (MQ=255)                                                                                                                                                                                                                                          tAGTACACAGTTCGTCGATAGTACCACAGCCGGTCAGACCCATACAGGAGCGCAGGCCGCCCATCTGCTGGTGAATGATCTCTTTCAGGCGACCTTTATAGGCTACGCGACCTTCGATTCCTTCCGGCACCAGTTTGTCGGCAGCGTTATCGCTCTGGAAATAACGGTCAGAGGAACCTTTGGACATCGCGCCCAGGGAACCCATACCACGGTAAGATTTGTAAGAACGGCCCTGGTGGAGTTCGATTTc  <  1:106192/250‑1 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                    GTTGAGTGTACTGAGAACCGAAGTCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACAAACTCCGCTTTAGTACGCAGTTCGTCGATAGTACCACAGCCGGTCAGACCCATACAGGAGCGCAGGCCGCCCATCTGCTGGTGAATGATCTCTTTCAGGCGACCTTTATAGGCTACGCGACCTTCGATACCTTCCGGCACCAGTTTGTCGGCAGCGTTATCGCTCTGGAAATAACGGTCAGAGGAACCTTTGGACATCGCGCCCAGGGAACCCATACCACGGTAAGATTTGTAAGAACGGCCCTGGTAGAGTTCGATTTC  >  NC_000913/2632472‑2632954

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑