Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,613,763	G→A	E622K (GAA→AAA)	hyfR →	DNA‑binding transcriptional activator HyfR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,613,763	0	G	A	100.0%	147.2 / NA	45	E622K (GAA→AAA)	hyfR	DNA‑binding transcriptional activator HyfR
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (21/24);  total (21/24)

AATCGCGCAATTGACGCCATCCCGACCGAGGCACTACGCCAGTTGATGTCGTGGGATTGGCCGGGCAACGTGCGCGAGCTGGAAAACGTGATTGAGCGGGCGGTACTGTTGACTCGTGGTAACAGTCTGAATTTACATCTAAATGTCCGACAAAGCCGTTTACTGCCGACGCTAAATGAAGATTCAGCGCTTCGCAGTTCAATGGCGCAGTTACTGCACCCGACGACGCCAGAGAATGACGAAGAAGAACGTCAGCGCATTGTTCA‑GGTATTGCGAGAAACCAATGGCATTGTTGCCGGGCCCCGTGGCGCAGCGACGCGATTAGGGATGAAGCGCACCACGCTGCTGTCACGAATGCAGCGGCTGGGGATCTCGGTTCGCGAGGTGTTGTAATCTGCTTTTGCAGGAGTATGCATGAGAAACAAACTCTCTTTCGACTTGCAGTTGAGCGCCAGAAAAGCGGCAATCGCT  >  NC_000913/2613520‑2613990                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                     aaTCGCGCAATTGACGCCATCCCGACCGAGGCACTACGCCAGTTGATGTCGTGGGATTGGCCGGGCAACGTGCGCGAGCTGGAAAACGTGATTGAGCGGGCGGTACTGTTGACTCGTGGTAACAGTCTGAATTTACATCTAAATGTCCGACAAAGCCGTTTACTGCCGACGCTAAATGAAGCTTCAGCGCTTCGCAGTTCAATGACGCAGTTACTGCACCCGACGCCGCCAGAGAAAGACGCAAAAGAACg                                                                                                                                                                                                                               >  2:263467/1‑251 (MQ=255)  aacgcgaaatgaacgacATCCCGACCGAGGCACTACGCCAGTGGAGGTCGAGGGATTGGGCGGGCAACGTGCGCGAGCTGGAAAATGTGATTGAGCGGGCGGTACTGTTGACTCGTGGTAACAGTCTGAATTTACATCTAAATGTCCGACAAAGCCGTTTACTGCCGACGCTAAATGAAGATGCACCGCTTCGAAGTTAAATGGCGCAGTTACTGAACCCGCCGACGCCAGAGAATGAGGAAAAAGAACGt                                                                                                                                                                                                                              <  2:232762/235‑1 (MQ=255)      cgcAATTGAAGCCATCCCGACCGAGGCACTACGCCAGTTGATGTCGTGGGATTGGCCGGGCAACGTGCGCGAGCTGGAAAACGTGATTGAGCGGGCGGTACTGTTGACTCGTGGTAACAGTCTGAATTTACATCTAAATGTCCGACAAAGCCGTTTACTGCCGACGCTAAATGACGATTCAGCGCTTCGCAGTTCAATGGCGCAGTTACTGCACCCGCCGTCGCCAGCGAATGACGCAAAAGAACGTCAgg                                                                                                                                                                                                                          >  2:96858/1‑250 (MQ=255)                   aTCCCGACCGAGGCACTACGCCAGTTGATGTCGTGGGATTGGCCGGGCAACGTGCGCGAGCTGGAAAACGTGATTGAGCGGGCGGTACTGTTGACTCGTGGTAACAGTCTGAATTTACATCTAAATGTCCGACAAAGCCGTTTACTGCCGACGCTAAATGAAGATTCAGCGCTTCGCAGTTCAATGGCGCAGTTACTGCACCCGACGCCGCCAGAGACTGACGTAAAAGAACGTCAGCGCATTGTTCA‑GGt                                                                                                                                                                                                            >  2:333926/1‑251 (MQ=255)                      ccgaccgaGGCACTACGCCAGTTGATGTCGGGGGATTGGCCGGGCAACGTGCGCGAGCTGGAAAACGTGATTGAGCTGGCGGTACTGTTGACTTGTGGTAACAGTCGGAATTTACATCTAAATGTCCGACAAAGCCGTTTACTGCCGACGCTAAATGAAGATTCAGCGCTTCGCAGTTCAATGGCGCAGTTACTGCACCCGACGACGCCAGAGAATGACGAAAAAGAACGTCAGCGCATTGTTCA‑GGTAtt                                                                                                                                                                                                         <  2:936/251‑1 (MQ=255)                        gaccgaGGCACTACGCCAGTTGATGTCGTGGGATTGGCCGGGCAACGTGCGCGAGCTGTAAAACGTGATTGAGCGTGCGGTACTGTTGACTCGTGGTAACAGTCTGAATTTACATCTAAATGTCCGACAAAGCCGTTTACTGCCGACGCTAAATGAAGATTCAGCGCTTCGCAGTTCAATGGCGCAGTTACTGCACCCGACGACGCCAGAGAATGACGAAAAAGAACGTCAGCGCATTGTTCA‑GGTATTGc                                                                                                                                                                                                       <  1:105049/251‑1 (MQ=255)                                       ccAGTTGATGTCGTGGGATTGGCCGGGCAACGTGCGCGAGCTGGAAAACGTGATTGAGCGGGCGGTACTGTTGACTCGTGGTAACAGTCTGAATTTACATCTAAATGTCCGACAAAGCCGTTTACTGCCGACGCTAAATGAAGATTCAGCGCTTCGCAGTTCAATGGCGCAGTTACTGCACCCGACGACGCCAGAGAATGACGAAAAAGAACGTCAGCGCATTGTTCA‑GGTATTGCGAGAAACCAATGGCa                                                                                                                                                                                        <  2:151340/251‑1 (MQ=255)                                            tGATGTCGTGGGATTGACCGGGCAACGTGCGCGAGCTGGAAAACGTGATTGAGCGGGCGGTACTGTTGACTCGTGGTAACAGTCTGAATTTACATCTAAATGTCCGACAAAGCCGTTTACTGCCGACGCTAACTGAAGATTCAGCGCTTCGCAGTTCAATGGCGCAGTTACTGCACCCGACGACGCCAGAGAATGCCGCAAAAGACCGTCAGCGCCTTGTTCA‑GGTATTGCGAGAAACCAATGACAttgtt                                                                                                                                                                                   >  2:398345/1‑251 (MQ=255)                                            tGATGGCGGGGGAGTGGCCGGGCAACGTGCGCGAGCTGGAAAACGTGATTGAGCGGGCGGTACTGTTGACTCGTGGTAACAGTCTGAATTTACATCTAAATGTCCGACAAAGCCGTTTACTGCCGACGCTAAATGAAGATTCAGCGCTTCGCAGTTCAATGGCGCAGTTACTGCACCCGACGACGCCAGAGAATGACGAAAAAGAACGTCAGCGCATTGTTCA‑GGTATTGCGAGAAACCAATGGCAttgt                                                                                                                                                                                    <  2:370270/250‑1 (MQ=255)                                             gATGTCGTGGGATTGGCCGGGCAACGTGCGCGAGCTGGAAAACGTGATTGAGCGGGCGGTACTGTTGACTCGTGGTAACAGTCCGAATTTACATCTAAATGTCCGACAACGCCGTTTACTGCCGGCGCTAACTGAAGATTCAGCGCGTCGCCGTTCAATGGCGCAGTTACTGCACCCGACGGCGCCAGAGAATGACGAAAAATGACGTCAGCGCATT‑TTCAAGGTATTGCGGGAAACAAAGGGCAtgggtg                                                                                                                                                                                  >  2:175624/1‑246 (MQ=255)                                                gTCGTGGGATTGGCCGGGCAACGTGCGCGAGCTGGAAAACGTGATTGAGCGGGCGGTACTGTTGACTCGTGGTAACAGTCTGAATTTACATCTAAATGTCCGACAAAGCCGTTTACTGCCGCCGCTAAATGAAGATTCAGCGCTTCGCAGTTCAATGGCGCAGTTACTGCACCCGACGACGCCAGAGAATGACGAAAAAGAACGTCAGCGCATTGTTCA‑GGTATTGCGAGAAACCAATGGCAGTGTTGCCg                                                                                                                                                                               >  2:388923/1‑251 (MQ=255)                                                                ggaaaCGGGCGCGAGCTGGAAAACGGGATTGAGCGGGCGGGACTGTTGACTCGTGTTAACAGTCTGAATTTACATCTAAATGTCCCACAAAGCCGTTTACTGCCGACGCTAAATGAAGATTCAGCGCTGCGCAGTGCAATGGCGCTGTTACTGCACCCGACGACGCCAGAGAATGACGAAAAAGAACGCCAGCGCATTGTTCA‑GGTATTGCGAGAAACCAATGCCATTTTTGCCGGGCCCCGTGGCGCAGc                                                                                                                                                               <  2:64280/248‑1 (MQ=255)                                                                  cAACGGGCGCGAGCTGGAAAACGTGATTGAGCGGGCGGTACTGTTGACTCGTGGTAACAGTCTGAATTTACATCTAAATGTCCGACAAAGCCGTTTACTGCCGACGCTAAATGAAGATTCAGCGCTTCGCAGTTCAATGGCGCAGTTACTGCACCCGACGACGCCAGAGAATGACGAAAAAGAACGTCAGCGCATTGTTCA‑GGTATTGCGAGAAACCAATGGCATTGTTGCCGGGCCCCGTGGCGCAGCGa                                                                                                                                                             <  2:27360/251‑1 (MQ=255)                                                                           cgAGCTGGAAAACGTGATTGAGCGGGCGGTACTGTTGACTCGTGGTAACAGTCTGAATTTACATCTAAATGTCCGACAAAGCCGTTTACTGCCGACGCTAAATGAAGATTCAGCGCTTCGCAGTTCAATGGCGCAGTTACTGCACCCGACGACGCCAGAGAATGACGAAAAAGAACGTCAGCGCATTGTTCA‑GGTATTGCGAGAAACCAATGGCATTGTTGCCGGGCCCCGTGGCGCAGCGACGCGAtt                                                                                                                                                      >  1:323120/1‑249 (MQ=255)                                                                              gCTGGAAAACGTGATTGAGCGGGCGGTACTGTTGACTCGTGGTAACAGTCTGAATTTACATCTAAATGTCCGACAAAGCCGTTTACTGCCGACGCTAAATGAAGATTCAGCGCTTCGCAGTTCAATGGCGCAGTTACTGCACCCGACGACGCCAGAGAATGACGAAAAAGAACGTCAGCGCATTGTTCA‑GGTATTGCGAGAAACCAATGGCATTGTTGCCGGGCCCCGTGGCGCAGCGACGCGATTAggg                                                                                                                                                  >  1:338542/1‑250 (MQ=255)                                                                               cTGGAAAACGTGATTGAGCGGGCGGTACTGTTGACTCGTGGTAACAGTCTGAATTTACATCTAAATGTCCGACAAAGCCGTTTACTGCCGACGCTAAATGAAGATTCAGCGCTTCGCAGTTCAATGGCGCAGTTACTGCACCCGACGACGCCAGAGAATGACGAAAAAGAACGTCAGCGCATTGTTCA‑GGTATTGCGAGAAACCAATGGCATTGTTGCCGGGCCCCGTGGCGCAGCGACGCGATTAGGGAt                                                                                                                                                >  1:349243/1‑251 (MQ=255)                                                                                 ggAAAACGTGATTGAGCGGGCGGTACTGTTGACGCGTGGTAACAGTCTGAATTTACATCTAAATGTCCGACAAAGCCGTTTACTGCCGACGCTAAATGAAGATTCAGCGCTTCGCAGTTCAATGGCGCAGTTACTGCACCCGACGACGCCAGAGAATGACGAAAAAGAACGTCAGCGCATTGTTCA‑GGTATTGCGAGAAACCAATGGCATTGTTGCCGGGCCCCGTGGCGCAGCGACGCGATTAGGGAt                                                                                                                                                <  1:276999/249‑1 (MQ=255)                                                                                        gTGATTGAGCGGGCGGTACTGTTGACTCGTGGTAACAGTCTGAATTTACATCTAAATGTCCGACAAAGCCGTTTACTGCCGACGCTAAATGAAGATTCAGCGCTTCGCAGTTCAATGGCGCAGTTACTGCACCCGACGACGCCAGAGAATGACGAAAAAGAACGTCAGCGCATTGTTCA‑GGTATTGCGAGAAACCAATGGCATTGTTGCCGGGCCCCGTGGCGCAGCGACGCGATTAGGGATGAAGCGcac                                                                                                                                       >  1:231957/1‑251 (MQ=255)                                                                                             ttaGCGTGCGGTACTGTTGATGCGGGGTAACATGCTTAATTTACAACAAAAGGTCCGACAACGCCGTTTACTGCCGACGCGAAATGATGATGCAGCGCTTCGCAGTTCAATGGCGCAGGTACTGCACCCGACGACGCCAGAGAAGGACGAAAAATAACGTCAGCGCATTGCTCA‑GGTATTGCGAGAAACCAATGGCATTGTTGCCGGGCCCCGTGGCGCAGCGACGCGAGTAGTGCTGAATCGCTCCACg                                                                                                                                   <  2:215860/248‑1 (MQ=255)                                                                                               aGCGGGCGGTACTGTTGCCTCGTGGTAACAGTCTGAATTTACATCTAAATGTCCGACAAAGCCGTTTACTGCCGACGCTAAATGAAGATTCAGCGCTTCGCAGTTCAATGGCGCAGTTACTGCACCCGACGACGCCAGAGAATGACGAAAAAGAACGTCAGCGCATTGTTCA‑GGTATTGCGAGCAACCAATGGCATTGTTGCCGGGCCCCGTGGCGAAGCGACGCGATTAGGGATGAAGCGCACCACgctg                                                                                                                                >  2:284404/1‑251 (MQ=255)                                                                                                          cTGTTGACTCGTGGTAACAGTCTGAATTTACATCTAAATGTCCGACAAAGCCGTTTACTGCCGACGCTAAATGAAGATTCAGCGCTTCGCAGTTCAATGGCGCAGTTACTGCACCCGACGACGCCAGAGAATGACGAAAAAGAACGTCAGCGCATTGTTCA‑GGTATTGCGAGAAACCAATGGCATTGTTGCCGGGCCCCGTGGCGCAGCGACGCGATTAGGGATGAAGCGCACCACGCTGCTGTCACGAAt                                                                                                                     >  1:239139/1‑251 (MQ=255)                                                                                                            gTTGACTCGTGGTAACAGTCTGAATTTACATCTAAATGTCCGACAAAGCCGTTTACTGCCGACGCTAAATGAAGATTCAGCGCTTCGCAGTTCAATGGCGCAGTTACTGCACCCGACGACGCCAGAGAATGACGAAAAAGAACGTCAGCGCATTGTTCA‑GGTATTGCGAGAAACCAATGGCATTGTTGCCGGGCCCCGTGGCGCAGCGACGCGATTAGGGATGAAGCGCACCACGCTGCTGTCACGAAt                                                                                                                     <  1:123504/249‑1 (MQ=255)                                                                                                                    gTGGTAACAGTCTGAATTTACATCTAAATGTCCGACAAAGCCGTTTACTGCCGACGCTAAATGAAGATTCAGCGCTTCGCAGTTCAATGGCGCAGTTACTGCACCCGACGACGCCAGAGAATGACGAAAAAGAACGTCAGCGCATTGTTCA‑GGTATTGCGAGAAACCAATGGCATTGTTGCCGGGCCCCGTGGCGCAGCGACGCGATTAGGGATGAAGCGCACCACGCTGCTGTCACGAATGCCGCGGCTg                                                                                                           >  2:59607/1‑251 (MQ=255)                                                                                                                                tGAATTTACATCTAAATGTCCGACAAAGCCGTTTACTGCCGACGCTAAATGAAGATTCAGCGCTTCGCAGTTCAATGGCGCAGTTACTGCACCCGACGACGCCAGAGAATGACGAAAAAGAACGTCAGCGCATTGTTCA‑GGTATTGCGAGAAACCAATGGCATTGTTGCCGGGCCCCGTGGCGCAGCGACGCGATTAGGGATGAAGCGCACCACGCTGCTGTCACGAATGCAGCGGCTGGGGATCTCGGtt                                                                                               >  1:404455/1‑251 (MQ=255)                                                                                                                                   aTTTACATCTAAATGTCCGACAAAGCCGTTTACTGCCGACGCTAAATGAAGATTCAGCGCTTCGCAGTTCAATGGCGCAGTTACTGCACCCGACGACGCCAGAGAATGACGAAAAAGAACGTCAGCGCATTGTTCA‑GGTATTGCGAGAAACCAATgg                                                                                                                                                                                          >  2:133469/1‑157 (MQ=255)                                                                                                                                   aTTTACATCTAAATGTCCGACAAAGCCGTTTACTGCCGACGCTAAATGAAGATTCAGCGCTTCGCAGTTCAATGGCGCAGTTACTGCACCCGACGACGCCAGAGAATGACGAAAAAGAACGTCAGCGCATTGTTCA‑GGTATTGCGAGAAACCAATgg                                                                                                                                                                                          <  1:133469/157‑1 (MQ=255)                                                                                                                                    tttACATCTAAATGTCCGACAAAGCCGTTTACTGCCGACGCTAAATGAAGATTCAGCGCTTCGCAGTTCAATGGCGCAGTTACTGCACCCGACGACGCCAGAGAATGACGAAAAAGAACGTCAGCGCATTGTTCA‑GGTATTGCGAGAAACCAATGGCATTGTTGCCGGGCCCCGTGGCGCAGCGACGCGATTAgg                                                                                                                                                   >  2:166775/1‑195 (MQ=255)                                                                                                                                    tttACATCTAAATGTCCGACAAAGCCGTTTACTGCCGACGCTAAATGAAGATTCAGCGCTTCGCAGTTCAATGGCGCAGTTACTGCACCCGACGACGCCAGAGAATGACGAAAAAGAACGTCAGCGCATTGTTCA‑GGTATTGCGAGAAACCAATGGCATTGTTGCCGGGCCCCGTGGCGCAGCGACGCGATTAgg                                                                                                                                                   <  1:166775/195‑1 (MQ=255)                                                                                                                                        cATCTAAATGTCCGACAAATCCGTTTACGGCCGACGCTAAATGAAGATTCAGCGCTTCGCAGTTCAATGGCGCAGTTACGGCACCCGACGACGCCAGAGAATGACGAAAAAGAACGTCAGCGCATTGTTCA‑GGTATTGCGAGAAACCAATGGCATTGTTGCCGGGCCCCGTGGCGCAGCGACGCGATTAGGGATGAAGCGCACCACGCTGCTGTCACGAATGCAGCGGCTGGGGATCTCGGTTCGCGAGgt                                                                                       <  2:397565/251‑1 (MQ=255)                                                                                                                                             aaagggccGACAAAGCCGTTTACTGCCGACGCTAAATGAAGATTCAGCGCTTCGCAGTTCAATGGCGCAGTTACTGCACCCGACGACGCCAGAGAATGACGAAAAAGAACGTCAGCGCATTGTTCA‑GGTATTGCGAGAAACCAATGGCATTGTTGCCGGGCCCCGTGGCGCAGCGACGCGATTAGGGATGAAGCGCACCACGCTGCTGTCACGAATGCAGCGGCTGGGGATCTCGGTTCGCGAGGtgtt                                                                                    <  2:349243/243‑1 (MQ=255)                                                                                                                                             aaaTGTCCGACAAAGCCGTTTACTGCCGACGCTAAATGAAGATTCAGCGCTTCGCAGTTCAATGGCGCAGTTACTGCACCCGACGACGCCAGAGAATGACGAAAAAGAACGTCAGCGCATTGTTCA‑GGTATTGCGAGAAACCAATGGCATTGTTGCCGGGCCCCGTGGCGCAGCGACGCGATTAGGGATGAAGCGCACCACGCTGCTGTCACGAATGCAGCGGCTGGGGATCTCGGTTCGCGAGGtgttg                                                                                   <  1:347348/250‑1 (MQ=255)                                                                                                                                              aaTGTCCGACAAAGCCGTTTACTGCCGACGCTAAATGAAGATTCAGCGCTTCGCAGTTCAATGGCGCAGTTACTGCACCCGACGACGCCAGAGAATGACGAAAAAGAACGTCAGCGCATTGTTCA‑GGTATTGCGAGAAACCAATGGCATTGTTGCCGGGCCCCGTGGCGCAGCGACGCGATTAGGGATGAAGCGCACCACGCTGCTGTCACGAATGCAGCGGCTGGGGATCTCGGTTCGCGAGGTGTTGTa                                                                                 <  1:78461/251‑1 (MQ=255)                                                                                                                                                 gTCCGACAAAGCCGTTTACTGCCGACGCTAAATGAAGATTCAGCGCTTCGCAGTTCAATGGCGCAGTTACTGCACCCGACGACGCCAGAGAATGACGAAAAAGAACGTCAGCGCATTGTTCA‑GGTATTGCGAGAAACCAATGGCATTGTTGCCGGGCCCCGTGGCGCAGCGACGCGATTAGGGATGAAGCGCACCACGCTGCTGTCACGAATGCAGCGGCTGGGGATCTCGGTTCGCGAGGTGTTGTAATc                                                                              >  2:376865/1‑251 (MQ=255)                                                                                                                                                   ccGACAAAGCCGTTTACTGCCGACGTTAAATGAAGATTCAGCGCTTCGCAGTTAAATGGCGCGTTTACTGCTCCCGACGACGCCAGAGAATGACGAAAAAGAACGTCAGCGCATTGTTCA‑GGTATTGCGAGAAACCAATGGCATTGTTGCCGGGCCCCGTGGCGCAGCGACGCGATTAGGGATGAAGCGCACCACGCTGCTGTCACGAATGCAGCGGCTGGGGATCTCGGTTCGCGAGGTGTTGTAATCTg                                                                            <  2:409468/251‑1 (MQ=255)                                                                                                                                                    cGACAAAGCCGTTTACTGCCGACGCGAAATGAAGATTCAGCGCTTCGCAGTTCAATGGCGCAGTTACTGCACCCGACGACGCCAGAGAATGACGAAAAATAACGTCAGCGCATTGTTCA‑GTTATTGCGAGAAACCAATGGCATTGTTGCCGGGCCCCGTGGCGCAGCGACGCGATTAGGGATGAAGCGCACCACGCTGCTGTCACGAATGCAGCGGCTGGGGATCTCGGTTCGCGAGGTGTTGTAATCTGc                                                                           <  1:303064/251‑1 (MQ=255)                                                                                                                                                         aaGCCGTTTACTGCCGACGCTAAATGAAGATTCAGCGCTTCGCAGTTCAATGGCGCAGTTACTGCACCCGACGACGCCAGAGAATGACGAAAAAGAACGTCAGCGCATTGTTCA‑GGTATTGCGAGAAACCAATGGCATTGTTGCCGGGCCCCGTGGCGCAGCGACGCGATTAGGGATGAAGCGCACCACGCTGCTGTCACGAATGCAGCGGCTGGGGATCTCGGTTCGCGAGGTGTTGTAATCTGCTTTTg                                                                      >  1:224133/1‑251 (MQ=255)                                                                                                                                                               tttACTGCCGACGCTAAATGAAGATTCAGCGCTTCGCAGTTCAATGGCGCAGTTACTGCACCCGACGACGCCAGAGAATGACGAAAAAGAACGTCAGCGCATTGTTCA‑GGTATTGCGAGAAACCAATGGCATTGTTGCCGGGCCCCGTGGCGCAGCGAc                                                                                                                                                            >  2:95476/1‑159 (MQ=255)                                                                                                                                                               tttACTGCCGACGCTAAATGAAGATTCAGCGCTTCGCAGTTCAATGGCGCAGTTACTGCACCCGACGACGCCAGAGAATGACGAAAAAGAACGTCAGCGCATTGTTCA‑GGTATTGCGAGAAACCAATGGCATTGTTGCCGGGCCCCGTGGCGCAGCGAc                                                                                                                                                            <  1:95476/159‑1 (MQ=255)                                                                                                                                                                 tACTGCCGACGCTAAATGAAGATTCAGCGCTTCGCAGTTCAATGGCGCAGTTACTGCACCCGACGACGCCAGAGAATGACGAAAAAGAACGTCAGCGCATTGTTCA‑GGTATTGCGAGAAACCAATGGCATTGTTGCCGGGCCCCGTGGCGCAGCGACGCGATTAGGGATGAAGCGCACCACGCTGCTGTCACGAATGCAGCGGCTGGGGATCTCGGTTCGCGAGGTGTTGTAATCTGCTTTTGCAGGAGt                                                               <  1:407278/250‑1 (MQ=255)                                                                                                                                                                     gCCGACGCTAAATGAAGATTCAGCGCTTCGCAGTTCAATGGCGCAGTTACTGCACCCGACGACGCCAGAGAATGACGAAAAAGAACGTCAGCGCATTGTTCA‑GGTATTGCGAGAAACCAATGGCATTGTTGCCGGGCCCCGTGGCGCAGCGACGCGATTAGGGATGAAGCGCACCACGCTGCTGTCACGAATGCAGCGGCTGGGGATCTCGGTTCGCGAGGTGTTGTAATCTGCTTTTGCAGGAGTATGc                                                           <  1:242761/250‑1 (MQ=255)                                                                                                                                                                                aTGAAGATTCAGCGCTTCGCAGTTCAATGGCGCAGTTACTGCACCCGACGACGCCAGAGAATGACGAAAAAGAACGTCAGCGCATTGTTCA‑GGTATTGCGAGAAACCAATGGCATTGTTGCCGGGCCCCGTGGCGCAGCGACGCGATTAGGGATGAAGCGCACCACGCTGCTGTCACGAATGCAGCGGCTGGGGACCTCGGTTCGCGAGGTGTTGTAATCTGCTTTTGCAGGAGTATGCATGAGaaacaaa                                               <  1:333926/251‑1 (MQ=255)                                                                                                                                                                                aTGAAGATTCAGCGCTTCGCAGATCAATGGCGCAGTTCCTGCACCCGACGACGCCAGAGAATGACGAAAAAGAACGTCAGCGCATTGTTCA‑GGTATTGCGAGAAACCAATGGCATTGTTGCCGGGCCCCGTGGCGCAGCGACGCGATTAGGGATGAAGCGCACCACGCTGCTGTCACGAATGCAGCGGATGGGGATCTCGGTTCGCGAGGTGTTGTAATCTGCTTTTGCAGGAGTATGCATGAGaaacaa                                                >  1:388523/1‑250 (MQ=255)                                                                                                                                                                                          aGCGCTTCGCAGTTCAATGGCGCAGTTACTGCACCCGACGACGCCAGAGAATGACGAAAAAGAACGTCAGCGCATTGTTCA‑GGTATTGCGAGAAACCAATGGCATTGTTGCCGGGCCCCGTGGCGCAGCGACGCGATTAGGGATGAAGCGCACCACGCTGCTGTCACGAATGCAGCGGCTGGGGATCTCGGTTCGCGAGGTGTTGTAATCTGCTTTTGCAGGAGTATGCATGAGAATCAAACTCTCTTTCg                                     >  1:403388/1‑251 (MQ=255)                                                                                                                                                                                                         aagggCGCAGTTACCGCACCCGACGACGCCAGAGAATGACGAAAAAGAACGGCAGCGCATTGTTCA‑GGTACTGCGAGAAACCAATGGCATTGTTGCCGGGCCCCGAGGCGCAGCGACGCGATTAGGGATGAAGCGCACCACGCTGCTGTCACGAATGCAGCGGCTGGGGATCTCGGTTCGCGAGGTGTTGTAATCTGCTTTTGCAGGAGTATGCATGAGAAACAAACTCTCTGTCGACTTGCAGTTGAgcg                      <  2:118386/248‑1 (MQ=255)                                                                                                                                                                                                                             cgacgaCGCCAGAGAATGACGAAAAAGAACGTCAGCGCATTGTTCA‑GGTATTGCGAGAAACCAATGGCATTGTTGCCGGGCCCCGTGGCGCAGCGACGCGATTAGGGATGAAGCGCACCACGCTGCTGTCACGAATGCAGCGGCTGGGGATCTCGGTTCGCGAGGTGTTGTAATCTGCTTTTGCAGGAGTATGCATGAGAAACAAACTCTCTTTCGACTTGCAGTTGAGCGCCAGAAAAGCGGCAATCGCt  <  1:306301/251‑1 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                     AATCGCGCAATTGACGCCATCCCGACCGAGGCACTACGCCAGTTGATGTCGTGGGATTGGCCGGGCAACGTGCGCGAGCTGGAAAACGTGATTGAGCGGGCGGTACTGTTGACTCGTGGTAACAGTCTGAATTTACATCTAAATGTCCGACAAAGCCGTTTACTGCCGACGCTAAATGAAGATTCAGCGCTTCGCAGTTCAATGGCGCAGTTACTGCACCCGACGACGCCAGAGAATGACGAAGAAGAACGTCAGCGCATTGTTCA‑GGTATTGCGAGAAACCAATGGCATTGTTGCCGGGCCCCGTGGCGCAGCGACGCGATTAGGGATGAAGCGCACCACGCTGCTGTCACGAATGCAGCGGCTGGGGATCTCGGTTCGCGAGGTGTTGTAATCTGCTTTTGCAGGAGTATGCATGAGAAACAAACTCTCTTTCGACTTGCAGTTGAGCGCCAGAAAAGCGGCAATCGCT  >  NC_000913/2613520‑2613990

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑