Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,600,978	C→T	intergenic (+30/‑223)	bcp → / → hyfA	thiol peroxidase/hydrogenase 4 component A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,600,978	0	C	T	95.2%	123.8 / ‑2.6	44	intergenic (+30/‑223)	bcp/hyfA	thiol peroxidase/hydrogenase 4 component A
Reads supporting (aligned to +/- strand):  ref base C (1/1);  new base T (18/22);  total (19/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.50e-01

GCTGCTTAACTTTACGCTCCTGTCTGATGAGGACCACCAGGTGTGCGAACAATTCGGCGTCTGGGGTGAAAAGTCCTTCATGGGCAAAACCTACGATGGCATTCATCGCATCAGCTTCCTGATTGACGCTGATGGCAAAATCGAACATGTCTTTGACGATTTCAAAACCAGCAATCACCACGACGTTGTGCTGAACTGGCTGAAAGAACACGCCTGATTACTTTGCTCCATTCCGTGCTGGCTGCGCTTGCGGCCAGCATACCTCACTTCTCGTGATCAAGATCACATTCTCGCTTTCCCCTGCGACACGGGTGTCGAATCCATTTTTTGCTGAACGTTAATGACCATCATTTTTGTACCGTTCAGAATCCAGTTAATACATAACTTATTGAATATATTGAGTTAATCAGAATGGCATCCTTTATGCAATATGAAATGCAATGTTTCATATCATTTTCAAGGAGCCGACATGAACCGCTTTGTGGTGGCC  >  NC_000913/2600732‑2601221                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                    gctgctTAACTTTACGCTCCTGTCTGATGAGGACCACCAGGTGTGCGAACAATTCGGCGTCTGGGGTGAAAAGTCCTTCATGGGCAAAACCTACGATGGCATTCATCGCATCAGCTTCCTGATTGACGCTGATGGCAAAATCGAACATGTCTTTGACGATTTCAAAACCAGCAATCACCACGACGTTGTGCTGAACTGGCTGAAAGAACACGCCTGATTACTTTGCGCCATTCCGTGCTGGCTGCGTTTGc                                                                                                                                                                                                                                                 <  1:423196/251‑1 (MQ=255)        aaCTTTACGCTCCTGTCTGATGAGGACCACCAGGTGTGCGAACAATTCGGCGTCTGGGGTGAAAAGTCCTTCATGGGCAAAACCTACGATGGCATTCATCGCATCAGCTTCCTGATTGACGCTGATGGCAAAATCGAACATGTCTTTGACGATTTCAAAACCAGCAATCACCACGACGTTGTGCTGAACTGGCTGAAAGAACACGCCTGATTACTTTGCTCCATTCCGTGCTGGCTGCGTTTGCGGCCAg                                                                                                                                                                                                                                           <  1:69333/250‑1 (MQ=255)                   tcTGTCTGATGAGGACCACCAGGTGGGCGAACAAGTCGGCGTCTGGGGTGAAAAGTCCTTCATGGGCAAAACCTACGATGGCATTCATCGCATCTGCTTCCTGATTGACGCTGATGGCAAAATCGATCATGTCTTTGACGATTTCAAAACCAGCAATCACCACGACGTTGTGCTGAACTGGCTGAAAGAACACGTCTGATTACTTGGCTCCATTCCGTGCTGGCTGCGTTTGCGGCCAGCATACCTCACt                                                                                                                                                                                                                                <  2:483696/249‑1 (MQ=255)                   ccTGTCTGATGAGGACCACCAGGTGTGCGAACAATTCGGCGTCTGGGGTGAAAAGTCCTTCATGGGCAAAACCTACGATGGCATTCATCGCATCAGCTTCCTGATTGACGCTGATGGCAAAATCGAACATGTCTTTGACGATTTCAAAACCAGCAATCACCACGACGTTGTGCTGAACTGGCTGAAAGAACACGCCTGATTACTTTGCTCCATTCCGTGCTGGCTGCGTTTGCGGCCAGCATACCTCAc                                                                                                                                                                                                                                 <  1:183334/249‑1 (MQ=255)                           atgaGGACCACCAGGTGTGCGAACAATTCGGCGTCTGGGGTGAAAAGTCCTTCATGGGCAAAACCTACGATGGCATTCATCGCATCAGCTTCCTGATTGACGCTGATGGCAAAATCGAACATGTCTTTGACGATTTCAAAACCAGCAATCACCACGACGTTGTGCTGAACTGGCTGAAAGAACACGCCTGATTACTTTGCTCCATTCCGTGCTGGCTGCGTTTGCGGCCAGCATACCTCACTTCTCGTGAt                                                                                                                                                                                                                       <  1:256473/251‑1 (MQ=255)                               ggACCACCAGGTGGGCGAACAATTCGGCGTCTGGGGTGAAAAGTCCTTCATGGGCAAAACCTACGATGGCAATCATCGCATCAGCTTCCTGATTGACGCTGATGGCAAAATCGAACATGTCTTTGACGATTTCAAAACCAGCAATCACCACGACGTTGTGCTGAACTGGCTGAAAGAACACGCCTGATTACTTTGCTCCATTCCGTGCTGGCTGCGTTTGCGGCCAGCATACCTCACTTCTCGTGATCAAg                                                                                                                                                                                                                   <  2:41587/251‑1 (MQ=255)                                      caGGTGTGCGAACAATTCGGCGTCTGGGGTGAAAAGTCCTTCATGGGCAAAACCTACGATGGCATTCATCGCATCAGCTTCCTGATTGACGCTGATGGCAAAATCGAACATGTCTTTGACGATTTCAAAACCAGCAATCACCACGACGTTGTGCTGAACTGGCTGAAAGAACACGCCTGATTACTTTGCTCCATTCCGTGCTGGCTGCGTTTGCGGCCAGCATACCTCACTTCTCGTGATCAAGATCACAt                                                                                                                                                                                                            >  1:345475/1‑251 (MQ=255)                                                   aaTTCGGCGTCTGGGGTGAAAAGTCCTTCATGGGCAAAACCTACGATGGCATTCATCGCATCAGCTTCCTGATTGACGCTGATGGCAAAATCGAACATGTCTTTGACGATTTCAAAACCAGCAATCACCACGACGTTGTGCTGAACTGGCTGAAAGAACACGCCTGATTACTTTGCTCCATTCCGTGCTGGCTGCGTTTGCGGCCAGCATACCTCACTTCTCGTGATCAGGATCACATTCTCGCTTTcccc                                                                                                                                                                                               >  2:81157/1‑251 (MQ=255)                                                    aTTCGGCGTCTGGGGTGAAAAGTCCTTCATGGGCAAAACCTACGATGGCATTCATCGCATCAGCTTCCTGATTGACGCTGATGGCAAAATCGAACATGTCTTTGACGATTTCAAAACCAGCAATCACCACGACGTTGTGCTGAACTGGCTGAAAGAACACGCCTGATTACTTTGCTCCATTCCGTGCTGGCTGCGTTTGCGGCCAGCATACCTCACTTCTCGTGATCAAGATCACATTCTCGCTTTCCCCt                                                                                                                                                                                              <  1:218189/251‑1 (MQ=255)                                                       cGGCGTCTGGGGTGAAAAGTCCTTCATGGGCAAAACCTACGATGGCATTCATCGCATCAGCTTCCTGATTGACGCTGATGGCAAAATCGAACATGTCTTTGACGATTTCAAAACCAGCAATCACCACGACGTTGTGCTGAACTGGCTGAAAGAACACGCCTGATTACTTTGCTCCATTCCGTGCTGGCTGCGTTTGCGGCCAGCATACCTCACTTCTCGTGATCAAGATCACATTCTCGCTTTCCCCTGCg                                                                                                                                                                                           >  1:93721/1‑251 (MQ=255)                                                       cGGCGTCTGGGGTGAAAAGTCCTTCATGGGCAAAACCTACGATGGCATTCATCGCATCAGCTTCCTGATTGACGCTGATGGCAAAATCGAACATGTCTTTGACGATTTCAAAACCAGCAATCACCACGACGTTGTGCTGAACTGGCTGAAAGAACACGCCTGATTACTTTGCTCCATTCCGTGCTGGCTGCGTTTGCGGCCAGCATACCTCACTTCTCGTGATCAAGATCACATTCTCGCTTTCCCCTGCg                                                                                                                                                                                           >  1:423843/1‑251 (MQ=255)                                                         gCGTCTGGGGTGAAAAGTCCTTCATGGGCAAAACCTACGATGGCATTCATCGCATCAGCTTCCTGATTGACGCTGATGGCAAAATCGAACATGTCTTTGACGATTTCAAAACCAGCAATCACCACGACGTTGTGCTGAACTGGCTGAAAGAACACGCCTGATTACTTTGCTCCATTCCGTGCTGGCTGCGTTTGCGGCCAGCATACCTCACTTCTCGTGATCAAGATCACATTCTCGCTTTCCCCTGCGac                                                                                                                                                                                         <  1:12587/251‑1 (MQ=255)                                                         gCGTCTGGGGTGAAAAGGCCTTCATGGGCAAAACCTACGATGGCATTCATCGCATCAGCTTCCTGATTGACGCTGATGGCAAAATCGAACATGTCTTTGACGATTTCAAAACCAGCAATCACCACGACGTTGTGCTGAACTGGCTGAAAGAACACGCCTGATTACTTTGCTCCATTCCGTGCTGGCTGCGTTTGCGGCCAGCATACCTCACTTCTCGTGATCAAGATCACATTCTCGCTTTCCCCTGCGac                                                                                                                                                                                         <  2:93721/251‑1 (MQ=255)                                                          cGTCTGGGGTGAAAAGTCCTTCATGGGCCAAACCTACGCTGGCATTCATCGCATCAGCTTCCTGATTGACGCTGATGGCAAAATCGAACATGTCTTTGACGATTTCAAAACCAGCAATCACCACGACGTTGTGCTGAACTGGCTGAAAGAACACGCCTGATTACTTTGCTCCATTCCGTGCTGGCTGCGTTTGCGGCCAGCATACCTCACTTCTCGTGATCAAGATCCCATTCTCGCTTTCCCCttcgccc                                                                                                                                                                                        >  2:457387/1‑245 (MQ=255)                                                              tGGGGTGAAAAGTCCTTCATGGGCAAAACCTACGATGGCATTCATCGCATCAGCTTCCTGCTTGACGCTAATGGCAAAATCGCACATGTCTTTGACGATTTCAAAACCAGCAATCACCACGACGTTGTGCTGAACTGTCTGAAAGAACACGCCTGATTACTTTGCTCCATTCCGTGCTGGCTGCGTTTGCGGCCAGCCTACCTCACTTCTCGTGATCAAGATCAAATTCTCGCTTTACCCTGCGTCACggg                                                                                                                                                                                    >  1:332253/1‑251 (MQ=255)                                                                   tGAAAAGTCCTTCATGGGCAAAACCTACGATGGCATTCCTCGCATCAGCTTCCTGATTGACGCTGATGGCAAAATCGAACATGTCTTTGACGATTTCAAAACCAGCAATCACCACGACGTTGTGCTGAACTGGCTGAAAGAACACGCCTGATTACTTTGCTCCATTCCGTGCTGGCTGCGTTTGCGGCCAGCATACCTCACTTCTCGTGATCAAGATCACATTCTCGCTTTCCCCTGCGACACGGGCGTCg                                                                                                                                                                               >  1:187003/1‑251 (MQ=255)                                                                    gAAAAGTCCTTCATGGGCAAAACCTACGATGGCATTCATCGCATCAGCTTCCTGATTGACGCTGATGGCAAAATCGAACATGTCTTTGACGATTTCAAAACCAGCAATCACCACGACGTTGTGCTGAACTGGCTGAAAGAACACGCCTGATTACTTTGCTCCATTCCGTGCTGGCTGCGTTTGCGGCCAGCCTACCTCACTTCTCGTGATCAAGATCACATTCTCGCTTTCCCCTGCGACACGGGAGTCg                                                                                                                                                                               >  1:266879/1‑250 (MQ=255)                                                                                                aTGGCATTCATCGCATCAGCTTCCTGATTGACGCTGATGGCAAAATCGAACATGTCTTTGACGATTTCAAAACCAGCAATCACCACGACGTTGTGCTGAACTGGCTGAAAGAACACGCCTGATTACTTTGCTCCATTCCGTGCTGGCTGCGTTTGCGGCCAGCATACCTCACTTCTCGTGATCAAGATCACATTCTCGCTTTCCCCTGCGACACGGGTGTCGAATCCATTTTTTGCTGAACGTTCATGAcc                                                                                                                                                  >  1:430792/1‑251 (MQ=255)                                                                                                aTGGCATTCATCGCATCAGCTTCCTGATTGACGCTGATGGCAAAATCGAACATGTCTTTGACGATTTCAAAACCAGCAATCACCACGACGTTGTGCTGAACTGGCTGAAAGAACACGCCTGATTACTTTGCTCCATTCCGTGCTGGCTGCGTTTGCGGCCAGCATACCTCACTTCTCGTGATCAAGATCACATTCGCGCTTTCCCCTGCGACACGGGTGTCGAATCCATTTTTTGCTGAACGTTAATGAcc                                                                                                                                                  <  2:74085/251‑1 (MQ=255)                                                                                                 tGGCATTCATCGCATCAGCTTCCTGATTGACGCTGATGGCAAAATCGAACATGTCTTTGACGATTTCAAAACCAGCAATCACCACGACGTTGTGCTGAACTGGCTGAAAGAACACGCCTGATTACTTTGCTCCATTCCGTGCTGGCTGCGCTTGCGGCCAGCATACCTCACTTCTCGTGATCAAGATCACATTCTCGCTTTCCCCTGCGACACGGGTGTCGAATCCATTTTTTGCTGAACGTTAATGAcc                                                                                                                                                  >  1:27291/1‑250 (MQ=255)                                                                                                    cATTCATCGCATCAGCTTCCTGATTGACGCTGATGGCAAAATCGAACATGTCTTTGACGATTTCAAAACCAGCAATCACCACGACGTTGTGCTGAACTGGCTGAAAGAACACGCCTGATTACTTTGCTCCATTCCGTGCTGGCTGCGTTTGCGGCCAGCATACCTCACTTCTCGTGATCAAGATCACATTCTCGCTTTCCCCTGCGACACGGGTGTCGAATCCATTTTTTGCTGAACGTTAATGACcatc                                                                                                                                               >  1:36520/1‑250 (MQ=255)                                                                                                                          aTTGACGCTGATGGCAAAATCGAACATGTCTTTGACGATTTCAAAACCAGCAATCACCACGACGTTGTGCTGAACTGGCTGAAAGAACACGCCTGATTACTTTGCTCCATTCCGTGCTGGCTGCGTTTGCGGCCAGCATACCTCACTTCTCGTGATCAAGATCACATTCTCGCTTTCCCCTGCGACACGGGTGTCGAATCCATTTTTTGCTGAACGTTAATGACCATCATTTTTGTACCGTTCAGAATcc                                                                                                                         >  1:406412/1‑250 (MQ=255)                                                                                                                              aCGCTGATGGCAAAATCGAACATGTCTTTGACGATTTCAAAACCGGCAAACACCCCGTCGTTGTGCTGAACTTGCTGAAAGAACACGCCTGATTACTTTGGTCCATTCCGTGCTGGCTGCGGTTGCGGACAGCATACCTCACTTCTCGTGATCAAGATCACATGCTCGCTTTCCCCTGCGACACGGGTGTCGAATCCATTTTGTGCTGAACGTTAATGAACATCATTTTTGGACCGTTCAGAATCCAGTTa                                                                                                                    <  2:169482/251‑1 (MQ=255)                                                                                                                                gcttatgtcAAAATCGAACATTGCTTGGAAGATTTCAAAACCAGCAATCACCACGACCTTGTGCTGAACTGGCTTAAAGAACACGCCTGATTACGTTGCTCCATTCCGTGCTGGCTGCGTTTGCGGCCAGCATGCCTCACTTCTCTTTATCAAGATCACATTCTCGCTTTCCCCTGCGACCCGGGTGTCGAATCCAGTTTTTGCTGAACGTTTATGACCATCATTTTTGTACCGTTCAGATTccttttcat                                                                                                                  <  2:319410/243‑8 (MQ=255)                                                                                                                                        cAAAATCGAACATGTCTTTGACGATTTCAAAACCAGCAATCACCACGACGTTGTGCTGAACTGGCTGAAAGAACACGCCTGATTACTTTGCTCCATTCCGTGCTGGCTGCGTTTGCGGCCAGCATACCTCACTTCTCGTGATCAAGATCACATTCTCGCTTTCCCCTGCGACACGGGTGTCGAATCCATTTTTTGCTGAACGTTAATGACCATCATTTTTGTACCGTTCAGAATCCAGTTAATACATAACt                                                                                                          >  1:385907/1‑251 (MQ=255)                                                                                                                                         aaaaTCGAACATGTCTTTGACGATTTCAAAACCAGCAATCACCACGACGTTGTGCTGAACTGGCTGAAAGAACACGCCTGATTACTTTGCTCCATTCCGTGCTGGCTGCGTTTGCGGCCAGCATACCTCACTTCTCGTGATCAAGATCACATTCTCGCTTTCCCCTGCGACACGGGTGTCGAATCCATTTTTTGCTGAACGTTAATGACCATCATTTTTGTACCGTTCAGAATCCAGTTAATACATAACtt                                                                                                         >  1:273740/1‑251 (MQ=255)                                                                                                                                                 aCATGTCTTTGACGATTTCAAAACCAGCAATCACCACGACGTTGTGCTGAACTGGCTGAAAGAACACGCCTGATTACGTTGCTCCATTCCGTGCTGGCTGCGTTTGCGGCCAGCATTCCTCACGTCTCGTGATCAAGATCACATTCTCGCTTTCCCCTGCGACACGGGTGTCGAATCCATTTTTTGCTGAACGTTAATGACCATCATTTTTGTACCGTTCAGAATCCAGTTAATACATAACTTATTGAata                                                                                                 <  2:193876/251‑1 (MQ=255)                                                                                                                                                   aTGTTTTTGAGGATTACAAAACCAGCAAGTACCACTACGTTTTGCTTAACCGGCTGACAGAACTCGTCTGATTACTTTTCTCCAATCCGGGTTGGCTGCGGTTGCTGCCATCATTCCTCTCTTCTCTTGATCAAGATCACATTCTCGCTTTCCCCTGCGACACTGGTGTCGAATCCATTTTTTGCTGAACGTTAATGTCCATCATTTTTGTACCGTTCAGAATCCAGTTAATTCATAACTTATTGAatatt                                                                                               <  2:257475/251‑2 (MQ=255)                                                                                                                                                     ttCTTTGACGATTTCAAAACCAGCAATCACCACGACGTTGTGCTGAACTGGCTGAAAGAACACGCCTGATGACTTTGCTCCATTCCGTGCTGGCTGCGTTTGCGGCCAGCATACCTCACTTCTCGTGATCAAGATCTCATTCTCGCTTTCCCCTGCGACACGGGTGTCGAATCCATTTTTTGCTGAACGTTAATGACCATCATTTTTGTACCGTTCAGAATCCAGTTAATACATAACTTATTGAatatat                                                                                              <  2:481092/249‑1 (MQ=255)                                                                                                                                                             cGATTTCAAAACCAGCAATCACCACGACGTTGTGCTGAACTGGCTGAAAGAACACGCCTGATTACTTTGCTCCATTCCGTGCTGGCTGCGTTTGCGGCCAGCATACCTCACTTCTCGTGATCAAGATCACATTCTCGCTTTCCCCTGCGACACGGGTGTCGAATCCATTTTTTGCTGAACGTTAATGACCATCATTTTTGTACCGTTCAGAATCCAGTTAATACATAACTTATTGAATATATTGAGTTAAt                                                                                     <  1:477457/251‑1 (MQ=255)                                                                                                                                                                   cAAAACCAGCAATCACCACGACGTTGTGCTGAACTGGCTGAAAGAACACGCCTGATTACTTTGCTCCATTCCGTGCTGGCTGCGTTTGCGGCCAGCATACCTCACTTCTCGTGATCAAGATCACATTCTCGCTTTCCCCTGCGACACGGGTGTCGAATCCATTTTTTGCTGAACGTTAATGACCATCATTTTTGTACCGTTCAGAATCCAGTTAATACATAACTTATTGAATATATTGAGTTAATCAGAAt                                                                               <  2:148435/251‑1 (MQ=255)                                                                                                                                                                                 accacGACGTTGTGCTGAACTGGCTGAAAGAACACGCCTGATTACTTTGCTCCATTCCGTGCTGGCTGCGTTTGCGGCAAGCATACCTCACTTCTAGTGTTCAAGATCACATTCTAGCATTCCACAGAGACACGGGTGTCGAATCCATTTTTTGCTGAACGTTAATGCCCATCATTTTTGTACCGTTCAGAATCAAGTTAATACATAACTTTTTGAATATATTGAGTTAATAAGAATGGCATCCTTTATGc                                                                 >  1:438732/1‑251 (MQ=255)                                                                                                                                                                                   cacGACGTTGTGCTGAACTGGCTGAAAGAACACGCCTGATTACTTTGCTCCATTCCGTGCTGGCTGCGTTTGCGGCCAGCATACCTCACTTCTCGTGATCAAGATCACATTCTCGCTTTCCCCTGCGACACGGGTGTCGAATCCATTTTTTGCTGAACGTTAATGACCATCATTTTTGTACCGTTCAGAATCCAGTTAATACATAACTTATTGAATATATTGAGTTAATCAGAATGGCATCCTTTATGCa                                                                >  1:79852/1‑250 (MQ=255)                                                                                                                                                                                       acgTTGTGCTGAACTGGCTGAAAGAACACGCCTGATTACTTTGCTCCATTCCGTGCTGGCTGCGTTTGCGGCCAGCATACCTCACTTCTCGTGATCAAGATCACATTCTCGCTTTCCCCTGCGACACGGGTGTCGAATCCATTTTTTGCTGAACGTTAATGACcatca                                                                                                                                              <  2:170221/168‑1 (MQ=255)                                                                                                                                                                                       acgTTGTGCTGAACTGGCTGAAAGAACACGCCTGATTACTTTGCTCCATTCCGTGCTGGCTGCGTTTGCGGCCAGCATACCTCACTTCTCGTGATCAAGATCACATTCTCGCTTTCCCCTGCGACACGGGTGTCGAATCCATTTTTTGCTGAACGTTAATGACcatca                                                                                                                                              >  1:170221/1‑168 (MQ=255)                                                                                                                                                                                        cgTTGTGCTGAACTGGCTGAAAGAACACGCCTGATTACTTTGCTCCATTCCGTGCTGGCTGCGTTTGCGGCCAGCATACCTCACTTCTCGTGATCTAGATCACATTCTCGCTTTCCCCTGCGACACGGGTGTCGAATCCATTTTTTGCTGAACGTTAATGACCATCATTTTTGTACCGTTCAGAATCCAGTTAATACATAACTTATTGAATATATTGAGTTAATCAGAATGGCATCCTTTATGCAATATg                                                           >  1:332516/1‑250 (MQ=255)                                                                                                                                                                                                   aCTGGCTGAAAGAACACGCCTGATTACTTTGCTCCATTCCGTGCTGGCTGCGTTTGCGGCCAGCATACCTCACTTCTCGTGATCAAGATCACATTCTCGCTTTCCCCTGCGACACGGGTGTCGAATCCATTTTTTGCTGAACGTTAATGACCATCATTTTTGTACCGTTCAGAATCCAGTTAATACATAACTTATTGAATATATTGAGTTAATCAGAATGGCATCCTTTATGCAATATGAAATGCAATGtt                                               <  2:423843/251‑1 (MQ=255)                                                                                                                                                                                                          gAAAGAACACGCCTGATTACTTTGCTCCATTCCGTGCTGGCTGCGTTTGCGGCCAGCATACCTCACTTCTCGTGATCAAGATCACATTCTCGCTTTCCCCTGCGACACGGGTGTCGAATCCATTTTTTGCTGAACGTTAATGACCATCATTTTTGTACCGTTCAGAATCCAGTTAATACATAACTTATTGAATATATTGAGTTAATCAGAATGGCATCCTTTATGCAATATGAAATGCAATGTTTCatat                                         <  2:36520/250‑1 (MQ=255)                                                                                                                                                                                                               aaCACGCCTGATTCCTTTGCTCCATTCCGTGCTGGCTGCGTTTGCGGCCAGCATACCTCACTTCTCGTGATCAAGATCACATTCTCGCTTTCCCCTGCGACACGGGTGTCGAATCCATTTTTTGCTGAACGTTAATGACCATCATTTTTGTACCGTTCAGAATCCAGTTAATACATAACTTATTGAATATATTGAGTTAATCAGAATGGCATCCTTTATGCAATATGAAATGCAATGTTTCATATCAtttt                                   <  1:54637/251‑1 (MQ=255)                                                                                                                                                                                                                acacGCCTGATTCCTTTGCTCCATTCCGTGCTGGCTGCGTTTTCGGCCAGCAACCCTCACTTCTCGTGATCAAGATCACTTTCTCGCTTGCCCCTGCGACACTGGTGTCGAATCCATTTTTTGCTGAACGTTAATGACCATCATTTTTGTACCGTTCAGAATCCAGTTAATACATAACTTATTGAATATATTGAGTTAATCAGAATGGCATCCTTTATGCAATATGAATTGCAATGTTTCATATCATTTTc                                  <  2:364169/251‑1 (MQ=255)                                                                                                                                                                                                                          ttACTTTGCTCCATTCCGTGCTGGCTGCGTTTGCGGCCAGCATACCTCACTTCTCGTGATCAAGATCACATTCTCGCTTTCCCCTGCGACACGGGTGTCGAATCCATTTTTTGCTGAACGTTAATGACCATCATTTTTGTACCGTTCAGAATCCAGTTAATACATAACTTATTGAATATATTGAGTTAATCAGAATGGCATCCTTTATGCAATATGAAATGCAATGTTTCATATCATTTTCAAGGAGCCg                         >  2:51746/1‑250 (MQ=255)                                                                                                                                                                                                                                             gctggctgCGTTTGCGGCCAGCATACCTCACTTCTCGTGATCAAGATCACATTCTCGCTTTCCCCTGCGACACGGGTGTCGAATCCATTTTTTGCTGAACGTTAATGACCATCATTTTTGTACCGTTCAGAATCCAGTTAATACATAACTTATTGAATATATTGAGTTAATCAGAATGGCATCCTTTATGCAATATGAAATGCAATGTTTCATATCATTTTCAAGGAGCCGACATGGACCGCTTTgtggt      <  1:92193/250‑1 (MQ=255)                                                                                                                                                                                                                                             gctggctgCGTTTGCGGCCAGCATACCTCACTTCTCGTGATCAAGATCACATTCTCGCTTTCCCCTGCGACACGGGTGTCGAATCCATTTTTTGCTGAACGTTAATGACCATCATTTTTGTACCGTTCAGAATCCAGTTAATACATAACTTATTGAATATATTGAGTTAATCAGAATGGCATCCTTTATGCAATATGAAATGCAATGTTTCATATCATTTTCAAGGAGCCGACATGAACCGCTTTgtggtg     <  1:81157/251‑1 (MQ=255)                                                                                                                                                                                                                                                ggctgCGCTGGCGGCCAGCATACCTCACTTCTCGTGATCAAGATCACATTCTCGCTTTCCCCTGCGACACGGGTGTCGAATCCATTTTTTGCTGAACGTTAATGACCATCATTTTTGTACCGTTCAGAATCCAGTTAATACATAACTTATTGAATATATTGAGTTAATCAGAATGGCATCCTTTATGCAATATGAAATGCAATGTTTCATATCATTTTCAAGGAGCCGACATGAACCGCTTTGTGGTGGcc  <  2:27291/251‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                    GCTGCTTAACTTTACGCTCCTGTCTGATGAGGACCACCAGGTGTGCGAACAATTCGGCGTCTGGGGTGAAAAGTCCTTCATGGGCAAAACCTACGATGGCATTCATCGCATCAGCTTCCTGATTGACGCTGATGGCAAAATCGAACATGTCTTTGACGATTTCAAAACCAGCAATCACCACGACGTTGTGCTGAACTGGCTGAAAGAACACGCCTGATTACTTTGCTCCATTCCGTGCTGGCTGCGCTTGCGGCCAGCATACCTCACTTCTCGTGATCAAGATCACATTCTCGCTTTCCCCTGCGACACGGGTGTCGAATCCATTTTTTGCTGAACGTTAATGACCATCATTTTTGTACCGTTCAGAATCCAGTTAATACATAACTTATTGAATATATTGAGTTAATCAGAATGGCATCCTTTATGCAATATGAAATGCAATGTTTCATATCATTTTCAAGGAGCCGACATGAACCGCTTTGTGGTGGCC  >  NC_000913/2600732‑2601221

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑