Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,577,937 T→C E147E (GAA→GAG)  maeB ← malate dehydrogenase (oxaloacetate‑decarboxylating) (NADP(+))

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,577,9370TC97.4% 121.9 / NA 39E147E (GAA→GAG) maeBmalate dehydrogenase (oxaloacetate‑decarboxylating) (NADP(+))
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base C (21/17);  total (21/18)
Fisher's exact test for biased strand distribution p-value = 4.62e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

GCCCTGATAGATAACGCCTTTTGAATCGCAAACCACGATGTTATGTTTTTGCAGACCCAGCGCTACCAGCAGGTTCATACAGGCGATTGCTGCGGCACCCGCGCCGGAAACCACCATCCGCACGTCGGAGATGTTTTTCTCCACCACGCGCAAGCCGTTGAGGATGGCGGCAGTGCTGATAATTGCCGTGCCGTGCTGATCGTCGTGGAATACCGGAATATTCATCCGCTCGCGCAGTTTCTGTTCAATATAGAAACATTCTGGCGCTTTAATGTCTTCGAGGTTGATGCCGCCGAAGGTTGGTTCGAGCGCGGCGACAACTTCAATAAATTTGTCCGGGTCGAGTTCGTCAACTTCAATGTCAAATACATCAATCCCGGCGAATTTCTTAAACAGAACGCCCTTGCCTTCCATCACCGGTTTGCCTGCCAGCGCGCCAATGTTGCCTAACCCCAGCACCGCCGTACCGT  >  NC_000913/2577694‑2578163
                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                  
gCCCTGATAGATAACGCCTTTTGAATCGCAAACCACGATGTTATGTTTTTGCAGACCCAGCGCTACCAGCAGGTTCATACAGGCGATTGCTGCGGCACCCGCGCCGGAAACCACCATCCGCACGTCGGAGATGTTTTTCTCCACCACGCGCAAGCCGTTGAGGATGGCGGCAGTGCTGATAATTGCCGTGCCGTGCTGATCGTCGTGGAATACCGGAATATTCATCCGCTCGCGCAGTTTCTGCTCAatat                                                                                                                                                                                                                             <  2:224488/251‑1 (MQ=255)
   cTGATAGATAACGCCTTTTGAATCGCAAACCACGATGTTATGTTTTTGCAGACCCAGCGCTACCAGCAGGTTCATACAGGCGATTGCTGCGGCACCCGCGCCGGAAACCACCATCCGCACGTCGGAGATGTTTTTCTCCACCACGCGCAAGCCGTTGAGGATGGCGGCAGTGCTGATAATTGCCGTGCCGTGCTGATCGTCGTGGAATACCGGAATATTCATCCGCTCGCGCAGTTTCTGCTCAatat                                                                                                                                                                                                                             >  1:168677/1‑248 (MQ=255)
     gatgtataACGCCTTTGGAATAGCAAACCACGATGTTAGGTTTTTGCAGACCCAGCGCAACCAGCAGGTTCATACTGGCGATTGCTTCGGCACCCGCGCCGGAAACCACCATCCGCACGGCGGAGAGGTTTTTCTCCACCACGCGCAAGCCGTTGAGGAAGGCGGCAGTGCTGATAATTGCCGTGCCGTGCTGATCGTCGTGGAATACCGGAATAGTCATCCGCTCGCGCAGTTTCTGTTCAATATAGaaa                                                                                                                                                                                                                        <  2:158524/246‑1 (MQ=255)
     gatagataACGCCTTTTGAATCGCAAACCACGATGTTATGTTTTTGCAGACCCAGCGCTACCAGCAGGTTCATACAGGCGATTGCTGCGGCACCCGCGCCGGAAACCACCATCCGCACGTCGGAGATGTTTTTCTCCACCACGCGCAAGCCGTTGAGGATGGCGGCAGTGCTGATAATTGCCGTGCCGTGCTGATCGTCGTGGAATACCGGACTATTCATCCGCTCGCGCAGTTTCTGCTCAATATAGaa                                                                                                                                                                                                                         >  1:158524/1‑250 (MQ=255)
        agataACGCCTTTTGAAGCGCAAACCACGATGTTATGTGTTTGCAGACCCAGCGCTACCAGCAGGTTCATACAGGCGATTGCTGCGGCACCCGCGCCGGAAACCACCATCCGCACGTCGGAGATGTTTTTCTCCACCACGCGCAAGCCGTTGAGGATGGCGGCAGTGCTGATAATTGCCGTGCCGTGCTGATCGTCGTGGAATACCGGAATATTCATCCGCTCGCGCAGTTTCTGCTCAATATAGAAACAt                                                                                                                                                                                                                     <  2:375617/251‑1 (MQ=255)
                     gtaaTCGCAACCCACGATGTTATGTTTTTGCAGACCCAGCGCGACCATCAGGTTCATACAGGCGATTGCTGCGGCACCCGCGCCGGAAACCACCATCCGCACGTCGGAGATGTTTTTCTCCACCACGCGCAAGCCGTTGAGGATGGCGGCAGTGCTGATAATTGCCGTGCCGTGCTGATCGTCGTGGAATACCGGAATATTCATCCGCTCGCGCAGTTTCTGCTCAATATAGAAACATTCTGGCGCTTTa                                                                                                                                                                                                         <  2:150489/248‑1 (MQ=255)
                       aaTCGAAAACAACGATGTTATGTTTTTGCAGACCCAGCGCTACCAGCAGGTTCATACAAGCAATTTCAGCGGCACCCGCGCCGGAAACAACCATCCGCACGTCGGCGATGTTTTTCTCCACCACGCGCAAGCCGTTGAGGATTGCGGAAGTGCTGGTAATTGCCGCGCCCTGCAGATCGTCGGGGACTAACGGAATATTCATCCGTACGCGCCGTTTCTGCTCAATATAGAAACATTCTGACGCTTTactg                                                                                                                                                                                                      >  2:33796/1‑248 (MQ=255)
                          cGCAAACCACGATGTTATGTTTTTGCAGACCCAGCGCTACCAGCAGGTTCATACAGGCGATTGCTGCGGCACCCGCGCCGGAAACCACCATCCGCACGTCGGAGATGTTTTTCTCCACCACGCGCAAGCCGTTGAGGATGGCGGCAGTGCTGATAATTGCCGTGCCGTGCTGATCGTCGTGGAATACCGGAATATTCATCCGCTCGCGCAGTTTCTGCTCAATATAGAAACATTCTGGCGCTTTAATGTCt                                                                                                                                                                                                   <  1:408061/251‑1 (MQ=255)
                                  ccgaagttatggtTTTGCGGCCCCAGCCCTGCAAGCAGGTTCATACAGGCGATTGCGGCGGCCCCCGCGCCGGAAACCACCATCCGCACGTCGGAGATGTTTTTCTCCACCACGCGCAAGCCGTTGAGGATGGCGGCAGTGCTGATAATTGCCGTGCCGTGCTTATCGTCGTGGAATACCGGAATATTCATCCGCTCGCGCAGTTTCTGCTCAATATAGAAACATTCTGGCGCTTTAATGTCTTCGAGGtt                                                                                                                                                                                           <  2:96750/246‑1 (MQ=255)
                                                    aGACCCAGCGCTACCAGCAGGTTCATACAGGCGATTGCTGCGGCACCCGCGCCGGAAACCACCATCCGCACGTCGGAGATGTTTTTCTCCACCACGCGCAAGCCGTTGAGGATGGCGGCAGTGCTGATAATTGCCGTGCCGTGCTGATCGTCGTGGAATACCGGAATATTCATCCGCTCGCGCAGTTTCTGCTCAATATAGAAACATTCTGGCGCTTTAATGTCTTCGAGGTTGATGCCGCCGAAggttgg                                                                                                                                                                         <  2:415832/251‑1 (MQ=255)
                                                      ccgctcttccaaacccagtttCATACAGGCGATTGCTGCGGCCCCCGCGCCGGAAACCACCATCCGCACGTCGGAGATGTTTTTCTCCACCACGCGCAAGCCGTTGAGGATGGCGGCAGTGCTGATAATTGCCGTGCCGTGCTGATCGTCGTGGAATACCGGAATATTCATCCGCTCGCGCAGTTTCTGCTCAATATAGAAACATTCTGGCGCTTTAATGTCTTCGAGGTTGATGCCGCCGAAggttggtt                                                                                                                                                                       <  2:325271/232‑1 (MQ=255)
                                                          aGCGCTACCAGCAGGTTCATACAGGCGATTGCTGCGGCACCCGCGCCGGAAACCACCATCCGCACGTCGGAGATGTTTTTCTCCACCACGCGCAAGCCGTTGAGGATGGCGGCAGTGCTGATAATTGCCGTGCCGTGCTGATCGTCGTGGAATACCGGAATATTCATCCGCTCGCGCAGTTTCTGCTCAATATAGAAACATTCTGGCGCTTTAATGTCTTCGAGGTTGATGCCGCCGAAGGTTGGTTCg                                                                                                                                                                     >  1:99202/1‑249 (MQ=255)
                                                                     cagGTTCATACAGGCGATTGCTGCGGCACCCGCGCCGGAAACCACCATCCGCACGTCGGAGATGTTTTTCTCCACCACGCGCAAGCCGTTGAGGATGGCGGCAGTGCTGATAATTGCCGTGCCGTGCTGATCGTCGTGGAATACCGGAATATTCATCCGCTCGCGCAGTTTCTGCTCAATATAGAAACATTCTGGCGCTTTAATGTCTTCGAGGTTGATGCCGCCGAAggttggtt                                                                                                                                                                       >  1:325271/1‑236 (MQ=255)
                                                                      agGTTCATACAGGCGATTGCTGCGGCACCCGCGCCGGAAACCACCATCCGCACGTCGGAGATGTTTTTCTCCACCACGCGCAAGCCGTTGAGGATGGCGGCAGTGCTGATAATTGCCGTGCCGTGCTGATCGTCGTGGAATACCGGAATATTCATCCGCTCGCGCAGTTTCTGCTCAATATAGaa                                                                                                                                                                                                                         <  2:65473/185‑1 (MQ=255)
                                                                      agGTTCATACAGGCGATTGCTGCGGCACCCGCGCCGGAAACCACCATCCGCACGTCGGAGATGTTTTTCTCCACCACGCGCAAGCCGTTGAGGATGGCGGCAGTGCTGATAATTGCCGTGCCGTGCTGATCGTCGTGGAATACCGGAATATTCATCCGCTCGCGCAGTTTCTGCTCAATATAGaa                                                                                                                                                                                                                         >  1:65473/1‑185 (MQ=255)
                                                                           caaacaggtggtttctgcGGCCGCCGCGCCGGAAACCACCATCCGCACGTCGGAGATGTTTTTCTCCCCCACGCGCAAGCCGTTGAGGATGGCGGCAGTGCTGATAATTGCCGTGCCGTGCTGATCGTCGTGGAATACCGGAATATTCATCCGCTCGCGCAGTTTCTGCTCAATATAGAAACATTCTGGCGCTTTAATGTCTTCGAGGTTGATGCCGCCGAAGGTTGGTTCGAGCGCGGCGACAACTTCa                                                                                                                                                   <  2:450283/236‑1 (MQ=255)
                                                                           cATACAGGCGATTGCTGCGGCACCCGCGCCGGAAACCACCATCCGCACGTCGGAGATGTTTTTCTCCACAACGCGCAAGCCGTTGAGGATGGCGGCAGTGCTGATAATTGCCGTGCCGTGCTGATCGTCGTGGAATACCGGAATATTCATCCGCTCGCGCAGTTTCTGCTCAATATAGAAACATTCTGCAGCTTTAATGTCTTCGAGGTTGATGCCGCCGAAGGTTGGTTAGAGCGCGGCGACAACTTCa                                                                                                                                                   >  1:466882/1‑250 (MQ=255)
                                                                                aGGCGATTGCTGCGGCACCCGCGCCGGAAACCACCATCCGCACGTCGGAGATGTTTTTCTCCACCACGCGCAAGCCGTTGAGGATGGCGGCAGTGCTGATAATTGCCGTGCCGTGCTGATCGTCGTGGAATACCGGAATATTCATCCGCTCGCGCAGTTTCTGCTCAATATAGAAACATTCTGGCGCTTTAATGTCTTCGAGGTTGATGCCGCCGAAGGTTGGTTCGAGCGCGGCGACAACTTCAATAAAt                                                                                                                                             >  1:182151/1‑251 (MQ=255)
                                                                                  gccaTTGTTGCGGCACCCGCGCCGGAAACCACCATCCGCACGTCGGAGATGTTTTTCTCCACCACGCGCAATCCGTTGAGGATGGCGGCAGTGCTGATAATTGCCGTGCCGGGCTGATCGTCGTGGAATACCGGAATATTCAGCCGCTCGCGCAGTTTCTGCTCAATATAGAAACATTCTGGCGCTTTAATGTCTTCGAGTTTGATGCCGCCGAAGGTTGGTTCGAGCGCGGCGACAACTTCAATAAAttt                                                                                                                                           <  2:40818/248‑1 (MQ=255)
                                                                                   cGATTGCTGCGGCACCCGCGCCGGAAACCACCATCCGCACGTCGGAGATGTTTTTCTCCACCACGCGCAAGCCGTTGAGGATGGCGGCAGTGCTGATAATTGCCGTGCCGTGCTGATCGTCGTGGAATACCGGAATATTCATCCGCTCGCGCAGTTTCTGCTCAATATAGAAACATTCTGGCGCTTTAATGTCTTCGAGGTTGATGCCGCCGAAGGTTGGTTCGAGCGCGGCGACAACTTCAATAAATTTg                                                                                                                                          >  1:186867/1‑251 (MQ=255)
                                                                                        gcttcGGCACCCGCCCCGGAAACCACCATCCGCACGGCGGAGATGTTTTTCACCACCACGCGCAAGCCGTTGAGGATGGCGGCAGTGCTGATAATTGCCGTGCCGTGCTGATCGTCGTGGAATACCGGAATATTCAGCCGCTCGCGCAGTTTCTGCTCAATATAGAAACATTCTGGCGCTTTAATGTCTTCGAGGTTGATGCCGCCGAAGGTTGGTTCGAGCGCGGCGACAACTTCAATAAATTTGTCCgg                                                                                                                                     <  2:205431/247‑1 (MQ=255)
                                                                                         ctgcGGCACCCGCGCCGGAAACCACCATCCGCACGTCGGAGATGTTTTTCTCCACCACGCGCAAGCCGTTGAGGATGGCGGCAGTGCTGATAATTGCCGTGCCGTGCTGATCGTCGTGGAATACCGGAATATTCATCCGCTCGCGCAGTTTCTGCTCAATATAGAAACATTCTGGCGCTTTAATGTCTTCGAGGTTGATGCCGCCGAAGGTTGGTTCGAGCGCGGCGACAACTTCAATAAATTTGTCCggg                                                                                                                                    >  2:65943/1‑251 (MQ=255)
                                                                                                   cgcgCCGGAAACCACCATCCGCACGTCGGAGATGTTTTTCTCCACCACGCGCAAGCCGTTGAGGATGGCGGCAGTGCTGATAATTGCCGTGCCGTGCTGATCGTCGTGGAATACCGGAATATTCATCCGCTCGCGCAGTTTCTGCTCAATATAGAAACATTCTGGCGCTTTAATGTCTTCGAGGTTGATGCCGCCGAAGGTTGGTTCGAGCGCGGCGACAACTTCCATAAATTTGTCCGGGTCGAGTTCGt                                                                                                                          >  2:256600/1‑251 (MQ=255)
                                                                                                    gcgcCGGAAACCACCATCCGCACGTCGGAGATGTTTTTCTCCACCACGCGCAAGCCGTTGAGGATGGCGGCAGTGCTGATAATTGCCGTGCCGTGCTGATCGTCGTGGAATACCGGAATATTCATCCGCTCGCGCAGTTTCTGCTCAATATAGAAACATTCTGGCGCTTTAATGTCTTCGAGGTTGATGCCGCCGAAGGTTGGTTCGAGCGCGGCGACAACTTCAATAAATTTGTCCGGGTCGAGTTCGTc                                                                                                                         <  1:59487/251‑1 (MQ=255)
                                                                                                            aaCCACCATCCGCACGTCGGAGATTTTTTTCTCCACCACGCGCAAGCCGTTGCGGATGGCGGCAGTGCTGATAATTCCCGTGCCGTGCTGATCGTCGTGGAATACCGGACTATTCATCCGCTCGCGCAGTTTCTGCGCCATATAGAAACATTCTGGCGCTTTAATGTCTTCGCGGTTGATGCCGCCGTAGGTTGGTTCGAGCGCGGCAACAACTTCAATGACTTTATCCGGGTCGAGTGCGTCCACTTCa                                                                                                                  >  2:398038/1‑250 (MQ=255)
                                                                                                             accaccATCCGCACGTCGGAGATGTTTATCTCCACCACGCGCAAGCCGTTGAGGATGGCGGCATGGCTGATAATTTCCTTGCCGTGCTGATCGTCGTGGAATACCGGAATATTCATCCGCTCGCGCAGTTTCTGCTCAATATAGAAACATTCTGGCGCTTTAATGTCTTCGAGGTTGATGCCGCCGAAGGTTGGTTCGAGCGCGGCGACAACTTCAAGAAATTTGTCCGGGTCGAGTTCGTCAACTTCAAt                                                                                                                <  2:325101/251‑1 (MQ=255)
                                                                                                                               gagaTGTTTTTCTCCACCACGCGCAAGCCGTTGAGGATGGCGGCAGTGCTGATAATTGCCGTGCCGTGCTGATCGTCGTGGAATACCGGAATATTCATCCGCTCGCGCAGTTTCTGCTCAATATAGAAACATTCTGGCGCTTTAATGTCTTCGAGGTTGATGCCGCCGAAGGTTGGTTCGAGCGCGGCGACAACTTCAATAAATTTGTCCGGGTCGAGTTCGTCAACTTCCATGTCAAAAACATCAATccc                                                                                              >  2:449689/1‑251 (MQ=255)
                                                                                                                                      tttactcCACCACGCGAAAGCCGTTGAGGATGGCGGCAGTGCTGATAATTGCCGTGCCGTGCTGATCGTCGTGGAATACCGGAATATTCATCCGCTCGCGCAGTTTCGGCTCAATATAGAAACATTCTGGCGCTTTAATGGCGTCGAGGTTGATGCCGCCGAAGGTTGGTTCGAGCGCGGCGAAAACTTCAATAAATTTGTCCGGGTCGAGTTCGTCAACTTCAATGTCAAATACATCAATCCCGGCGaa                                                                                        <  1:449689/246‑1 (MQ=255)
                                                                                                                                         tctcCACCACGCGCAAGCCGTTGAGGATGGCGGCAGTGCTGATAATTGCCGTGCCGTGCTGATCGTCGTGGAATACCGGAATATTCATCCGCTCGCGCAGTTTCTGCTCAATATAGAAACATTCTGGCGCTTTAATGTCTTCGAGGTTGATGCCGCCGAAGGTTGGTTCGAGCGCGGCGACAACTTCAATAAATTTGTCCGGGTCGAGTTCGTCAACTTCAATGTCAAATACATCAATCCCGGCGAATTTc                                                                                    >  1:188917/1‑251 (MQ=255)
                                                                                                                                             caccacGCGCAAGCCGTTGAGGATGGCGGCAGTGCTGATAATTGCCGTGCCGTGCTGATCGTCGTGGAATACCGGAATATTCATCCGCTCGCGCAGTTTCTGCTCAATATAGAAACATTCTGGCGCTTTAATGTCTTCGAGGTTGATGCCGCCGAAGGTTGGTTCGAGCGCGGCGACAACTTCAATAAATTTGTCCGGGTCGAGTTCGTCAACTTCAATGTCAAATACATCCATCCCGGCGAATTTCTTa                                                                                 >  2:139334/1‑250 (MQ=255)
                                                                                                                                                         gCCGTTGAGGATGGCGGCAGTGCTGATAATTGCCGTGCCGTGCTGATCGTCGTGGAATACCGGAATAGTCAACCGCTCGCGCAGTTTCTGCTCAATATAGAAACATTCTGGCGCTTTAATGTCTTCGAGGTTGATGCCGCCGAAGGTTGGTTCGAGCGCGGCGACAACTTCAATAAATTTGTCCGGGTCGAGTTCGACAACTTCAATGTCAAATACATCAATCCCGGCGAATTTCTTAAACAGAACGCCCt                                                                    <  2:125079/251‑1 (MQ=255)
                                                                                                                                                                   aTGGCGGCAGTGCTGATAATTGCCGTGCCGTGCTGATCGTCGTGGAATACCGGAATATTCATCCGCTCGCGCAGTTTCTGCTCAATATAGAAACATTCTGGCGCTTTAATGTCt                                                                                                                                                                                                   <  2:282681/114‑1 (MQ=255)
                                                                                                                                                                   aTGGCGGCAGTGCTGATAATTGCCGAGCCGTGCTGATCGTCGTGGAATACCGGAATATTCCTCCGCTCGCGCAGTTTCTGCTCAATATAGAAACATTCTGGCGCTTTAATGTCt                                                                                                                                                                                                   >  1:282681/1‑114 (MQ=255)
                                                                                                                                                                                tGATAATTGCCGTGCCGTGCTGATCGTCGTGGAATACCGGAATATTCATCCGCTCGCGCAGTTTCTGCTCAATATAGAAACATTCTGGCGCTTTAATGTCTTCGAGGTTGATGCCGCCGAAGGTTGGTTCGAGCGCGGCGACAACTTCAATAAGTTTGTCCGGGTCGGGTTCGTCAACTTCAATGTCAAATACATCCATCCCGGCGAATTTCTTAAACAGAACGCCCTTGCCTTCCATCACCGGttagcat                                             >  2:322511/1‑246 (MQ=255)
                                                                                                                                                                                           gtgccgtgcTGATCGTCGTGGAATACCGGAATATTCATCCGCTCGCGCAGTTTCTGCTCAATATAGAAACATTCTGGCGCTTTAATGTCTTCGAGGTTGATGCCGCCGAAGGTTGGTTCGAGCgcggc                                                                                                                                                             >  2:232850/1‑128 (MQ=255)
                                                                                                                                                                                           gtgccgtgcTGATCGTCGTGGAATACCGGAATATTCATCCGCTCGCGCAGTTTCTGCTCAATATAGAAACATTCTGGCGCTTTAATGTCTTCGAGGTTGATGCCGCCGAAGGTTGGTTCGAGCgcggc                                                                                                                                                             <  1:232850/128‑1 (MQ=255)
                                                                                                                                                                                                              ggAATACCGGAATATTCATCCGCTCGCGCAGTTTCTGCTCAATATAGAAACATTCTGGCGCTTTAATGTCTTCGAGGTTGATGCCGCCGAAGGTTGGTTCGAGCGCGGCGACAACTTCAATAAATTTGTCCGGGTCGAGTTCGTCAACTTCAATGTCAAATACATCAATCCCGGCGAATTTCTTAAACAGAACGCCCTTGCCTTCCATCACCGGTTTGCCTGCCAGCGCGCCAATGTTGCCTAACCCCAGc               >  2:176838/1‑251 (MQ=255)
                                                                                                                                                                                                              ggAATACAGGAATATTCATCCGCTCGCGCAGTTTCTGCTCAATATAGAAACGTTCTGGCGCTTTAATGTCTTCGCGGTTGCTGCCGCCGAAGGTTGGTTCGAGCGCGGCGACAACTTCAATAAATTTGTCCGGGTCGAGTTCGTCAACTTCAATGTCAAATACATCAATCCCGGCGAATTTCTTAAACAGAACGCCCTTGCCTTCCATCACCGGTTTGCCTGCCAGCGCGCCAATGTTGCCTAACCCCAGc               >  2:200056/1‑251 (MQ=255)
                                                                                                                                                                                                                           atTCAACCGCTCGCGCAGTTGCTGCACAATATAGAAACATTCAGGCGAGTTAATGTCTTCGAGGTTGATGCCGCCGAAGGTTGGATCGTGCGCGGCGACAGCTTCAATAAATTTGTCCGGGTCGAGTTCGTCAACTTCAATGTCAAATACATCAATCCCGGCGAATTTCTTAAACAGAACGCCCTTGCCTTCCATCACCGTTTGGCCGGCCAGCGCACCAATGTAGCCTAACCCCAGAACCCCCGTACCGt  >  2:458833/1‑251 (MQ=255)
                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                  
GCCCTGATAGATAACGCCTTTTGAATCGCAAACCACGATGTTATGTTTTTGCAGACCCAGCGCTACCAGCAGGTTCATACAGGCGATTGCTGCGGCACCCGCGCCGGAAACCACCATCCGCACGTCGGAGATGTTTTTCTCCACCACGCGCAAGCCGTTGAGGATGGCGGCAGTGCTGATAATTGCCGTGCCGTGCTGATCGTCGTGGAATACCGGAATATTCATCCGCTCGCGCAGTTTCTGTTCAATATAGAAACATTCTGGCGCTTTAATGTCTTCGAGGTTGATGCCGCCGAAGGTTGGTTCGAGCGCGGCGACAACTTCAATAAATTTGTCCGGGTCGAGTTCGTCAACTTCAATGTCAAATACATCAATCCCGGCGAATTTCTTAAACAGAACGCCCTTGCCTTCCATCACCGGTTTGCCTGCCAGCGCGCCAATGTTGCCTAACCCCAGCACCGCCGTACCGT  >  NC_000913/2577694‑2578163

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑