Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,388,753	G→A	G40D (GGT→GAT)	yfbO →	uncharacterized protein YfbO

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,388,753	0	G	A	100.0%	57.9 / NA	19	G40D (GGT→GAT)	yfbO	uncharacterized protein YfbO
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (8/11);  total (8/11)

TCCTGCTTAACATTCATGTTATGATGCTCTTGAAACTAATAATACGTATGGAATTGTCACTATTTATCTGCTAATGAATTCAAACAATGTCATTCGTTTGATGAATATATTATTTCAGGGAATAATTATGACACCTTTAGAACGCATTACCCAACTTGTTAATATCAATGGTGATGTTAATAATCCTGATACTCCTCGTCCATTGTTATCTCTTGAGGATTTTTTTATTGACAATAATATCCATGGTTCAATATGTTGCAATGTGATTCCAGAACAATCTCCGCAAGCTATCTATCACCATTTTCTGAAAATACGTGAGCGTAACAATGTTAGTGATGTTTTGGTAGAAATTACGATGTTCGATGACCCAGACTGGCCCTTTAGTGAAAGTATTTTAGTTATCACCACTGCTTCACCAGAAGAAGTTCAGAGCTGGTTTGTAGAAGAAATTGCCCCTGATGAGTGCTGGGAAGGATGGTCAGAAGAT  >  NC_000913/2388508‑2388994                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                  tCCTGCTTAACATTCATGTTATGATGCTCTTGAAACTAATAATACGTATGGAATTGTCACTATTTATCTGCTAATGAATTCAAACAATGTCATTCGTTTGATGAATATATTATTTCAGGGAATAATTATGACACCTTTAGAACGCATTACCCAACTTGTTAATATCAATGGTGATGTTAATAATCCTGATACTCCTCGTCCATTGTTATCTCTTGAGGTTTTTTTTATTGACAATAATATCCATGATTCa                                                                                                                                                                                                                                               >  2:432114/1‑250 (MQ=255)     gCTTAACATTCATGTTATGATGCTCTTGAAACTAATCATACGGATGGTATTGTCACTATTTATCTGCTAATGAATTCAACCAATGTCATTCGTTTGATGAATATATTATTTCCGGGAATAATTATGACACCTTAAGAATGCATTACCCAACTTGTTCATATCAATGGTGATGTTAATAATCCTGATCCTCCTCGTCCATTGTTATCTCTTGAGGATTTTTTTATTGACACTAATATCCATGATTCAatatt                                                                                                                                                                                                                                          >  2:120480/1‑250 (MQ=255)              tCATGTTATGATGCTCTTGAAACTAATAATACGTATGGAATTGTCACTATTTATCTGCTAATGAATTCAAACAATGTCATTCGTTTGATGAATATATTATTTCAGGGAATAATTATGACACCTTTAGAACGCATTACCCAACTTGTTAATATCAATGGTGATGTTAATAATCCTGATACTCCTCGTCCATTGTTATCTCTTGAGGATTTTTTTATTGACAATAATATCCATGATTCAATATGTTGCAAtg                                                                                                                                                                                                                                  <  1:198487/250‑1 (MQ=255)                                                     agtggcACTATTTATCTGCTAATGAATTCAAACACTGTCATTCGTTTGATGAATATATTATTTAAGGGAATAATTATGACACCTTTAGAACGCATTACCCAAATTGTTAATATCAATGGTGATGTTAATAATCCTGCTACTCCTCGTCCATTGTTCTCTATTGAGGCTTTTTTTATTGACAATAATATCCATGATTCAATATTTTGCAATGTGATTCCAGAACAATCTCCGCAAGCTATCTATCACCAttt                                                                                                                                                                                          >  2:416101/6‑251 (MQ=255)                                                              aTTTATCTGCTAATGAATTCAAACAATGTCATTCGTTTGATGAATATATTATTTCAGGGAATAATTATGACACCTTTAGAACGCATTACCCAACTTGTTAATATCAATGGTGATGTTAATAATCCTGATACTCCTCGTCCATTGTTATCTCTTGAGGATTTTTTTATTGACAATAATATCCATGATTCAATATGTTGCAATGTGATTCCAGAACAATCTcc                                                                                                                                                                                                               >  1:415982/1‑221 (MQ=255)                                                              aTTTATCTGCTAATGAATTCAAACAATGTCATTCGTTTGATGAATATATTATTTCAGGGAATAATTATGACACCTTTAGAACGCATTACCCAACTTGTTAATAGCAATGGTGATGTTAATAATCCTGATACTCCTCGTCCATTGTTATCTCTTGAGGATTTTTTTATTGACAATAATATCCATGATTCAATATGTTGCAATGTGATTCCAGAACAATCTcc                                                                                                                                                                                                               <  2:415982/221‑1 (MQ=255)                                                                          aTGAATTCAAACAATGTCATTCGTTTGATGAATATATTATTTCAGGGAATAATTATGACACCTTTAGAACGCATTACCCAACTTGTTAATATCAATGGTGATGTTAATAATCCTGATACTCCTCGTCCATTGTTATCTCTTGAGGATTTTTTTATTGACAATAATATCCATGATTCAATATGTTGCAATGTGATTCCAGAACAATCTCCGCAAGCTATCTATCACCATTTTCTGAAAATACGTGAGCGTa                                                                                                                                                                      >  1:360933/1‑250 (MQ=255)                                                                                 cAAACAATGTCATTCGTTTGATGAATATATTATTTCAGGGAATAATTATGACACCTTTAGAACGCATTACCCAACTTGTTAATATCAATGGTGATGTTAATAATCCTGATACTCCTCGTCCATTGTTATCTCTTGAGGATTTTTTTATTGACAATAATATCCATGATTCAATATg                                                                                                                                                                                                                                          <  1:219848/175‑1 (MQ=255)                                                                                 cAAACAATGTCATTCGTTTGATGAATATATTATTTCAGGGAATAATTATGACACCTTTAGAACGCATTACCCAACTTGTTAATATCAATGGTGATGTTAATAATCCTGATACTCCTCGTCCATTGTTATCTCTTGAGGATTTTTTTATTGACAATAATATCCATGATTCAATATg                                                                                                                                                                                                                                          >  2:219848/1‑175 (MQ=255)                                                                                           cATTCGTTTGATGAATATATTATTTCAGGGAATAATTATGACACCTTTAGAACGCATTACCCAACTTGTTAATATCAATGGTGATGTTAATAATCCTGATACTCCTCGTCCATTGTTATCTCTTGAGGATTTTTTTATTGACAATAATATCCATGATTCAATATGTTGCAATGTGATTCCAGAACAATCTCCGCAAGCTATCTATCACCATTTTCTGAAAATACGTGAGCGTAACAATGTTAGTGATGtt                                                                                                                                                     <  2:133594/250‑1 (MQ=255)                                                                                                        aaTATATTATTTCAGGGAATAATTATGACACCTTTAGAACGCATTACCCAACTTGTTAATATCAATGGTGATGTTAATAATCCTGATACTCCTCGTCCATTGTTATCTCTTGAGGATTTTTTTATTGACAATAATATCCATGATTCAATATGTTGCAATGTGATTCCAGAACAATCTCCGCAAGCTATCTATCACCATTTTCTGAAAATACGTGAGCGTAACAATGTTAGTGATGTTTTGGTAGAAATTAc                                                                                                                                       <  1:183068/251‑1 (MQ=255)                                                                                                                                                          aCTTGTTAATATCAATGGTGATGTTAATAATCCTGATTCTCCTCGTCCATTGTTATCTCTTGAGGATTTTTTTATTGACAATAATATCCATGATTCAATATGTTGCAATGTGATTCCAGAACAATCTCCGCAAGCTATCTATCACCATTTTCTGAAAATACGTGAGCGTAACAATGTTAGTGATGTTTTGGTAGAAATTACGATGTTCGATGACCCAGACTGGCCCTTTAGTGAAAGTATTTTAGTTATca                                                                                     <  1:324076/251‑1 (MQ=255)                                                                                                                                                                                                      gTCCATTGTTATCTCTTGAGGATTTTTTTATTGACAATAATATCCATGATTCAATATGTTGCAATGTGATTCCAGAACAATCTCCGCAAGCTATCTATCACCATTTTCTGAAAATACGTGAGCGTAACAATGTTAGTGATGTTTTGGTAGAAATTACGATGTTCGATGACCCAGACTGGCCCTTTAGTGAAAGTATTTTAGTTATCACCACTGCTTCACCAGAAGAAGTTCAGAGCTGGTTTGTAgaagaa                                         <  1:173673/251‑1 (MQ=255)                                                                                                                                                                                                             gTTATCTCTTGAGGATTTTTTTATTGACAATAATATCCATGATTCAATATGTTGCAATGTGATTCCAGAACAATCTCCGCAAGCTATCTATCACCATTTTCTGAAAATACGTGAGCGTAACAATGTTAGTGATGTTTTGGTAGAAATTACGATGTTCGATGACCCAGACTGGCCCTTTAGTGAAAGTATTTTAGTTATCACCACTGCTTCACCAGAAGAAGTTCAGAGCTGGTTTGTAGAAGAAATTGccc                                  <  1:429150/251‑1 (MQ=255)                                                                                                                                                                                                                          gATTTTTTTATTGACAATAATATCCATGATTCAATATGTTGCAATGTGATTCCAGAACAATCTCCGCAAGCTATCTATCACCATTTTCTGAAAATACGTGAGCGTAACAATGTTAGTGATGTTTTGGTAGAAATTACGATGTTCGATGACCCAGACGGGCCCTTTAGTGAAAGTATTTTAGTTATCACCACGGCTTCACCAGAAGAAGTTCAGAGCTGGTTTGTAGAAGAAATTGCCCCTGATGAGTGCTg                     <  1:306107/251‑1 (MQ=255)                                                                                                                                                                                                                           aTTTTTTTATTGACAATAATATCCATGATTCAATATGTTGCAATGTGATTCCAGAACAATCTCCGCAAGCTATCTATCACCATTTTCTGAAAATACGTGAGCGTAACAATGTTAGAGATGTTTTGGTAGAAATTACGATGTTCGATGACCCAGACTGGCCCTTTAGTGAAAGTATTTTAGTTATCACCACTGCTTCACCAGAAGAGTTTCAGAGCTGGTTTGTAGAAGAAATTGCCCCTGATGAGAGCTgg                    >  1:124007/1‑251 (MQ=255)                                                                                                                                                                                                                                   aTTGACAATAATATCCATGATTCAATATGTTGCAATGTGATTCCAGCACAATCTCCGCAAGCTATCTATCACCATTTTCTGAAAATACGTGAGCGTAACAATGTTAGTGATGTTTTGGTAGAAATTACGATGTTCGATGACCCAGACTGGCCCTTTAGTGAAAGTATTTTAGTTATCACCACTGCTTCACCAGAAGAAGTTCAGAGCTGGTTTGTAGAAGAAATTGCCCCTGATGAGTGCTGGGAAGGATg            >  2:345052/1‑251 (MQ=255)                                                                                                                                                                                                                                            aataTCCATGATTCAATATGTTGCAATGTGATTCCAGAACAATCTCCGCAAGCTATCTATCACCATTTTCTGAAAATACGTGAGCGTAACAATGTTAGTGATGTTTTGGTAGAAATTACGATGTTCGATGACCCAGACTGGCCCTTTAGTGAAAGTATTTTAGTTATCACCACTGCTTCACCAGAAGAAGTTCAGAGCTGGTTTGTAGAAGAAATTGCCCCTGATGAGTGCTGGGAAGGATGGTCagaag    <  2:265337/250‑1 (MQ=255)                                                                                                                                                                                                                                             atatCCATGATTCAATATGTTGCAATGTGATTCCAGAACAATCTCCGCAAGCTATCTATCACCATTTTCTGAAAATACGTGAGCGTAACAATGTTAGTGATGTTTTGGTAGAAATTACGATGTTCGATGACCCAGACTGGCCCTTTAGTGAAAGTATTTTAGTTATCACCACTGCTTCACCAGAAGAAGTTCAGAGCTGGTTTGTAGAAGAAATTGCCCCTGATGAGTGCTGGGAAGGATGGTCAGAAGAt  <  2:360933/251‑1 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                  TCCTGCTTAACATTCATGTTATGATGCTCTTGAAACTAATAATACGTATGGAATTGTCACTATTTATCTGCTAATGAATTCAAACAATGTCATTCGTTTGATGAATATATTATTTCAGGGAATAATTATGACACCTTTAGAACGCATTACCCAACTTGTTAATATCAATGGTGATGTTAATAATCCTGATACTCCTCGTCCATTGTTATCTCTTGAGGATTTTTTTATTGACAATAATATCCATGGTTCAATATGTTGCAATGTGATTCCAGAACAATCTCCGCAAGCTATCTATCACCATTTTCTGAAAATACGTGAGCGTAACAATGTTAGTGATGTTTTGGTAGAAATTACGATGTTCGATGACCCAGACTGGCCCTTTAGTGAAAGTATTTTAGTTATCACCACTGCTTCACCAGAAGAAGTTCAGAGCTGGTTTGTAGAAGAAATTGCCCCTGATGAGTGCTGGGAAGGATGGTCAGAAGAT  >  NC_000913/2388508‑2388994

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑