Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	774	T→C	T146T (ACT→ACC)	thrA →	fused aspartate kinase/homoserine dehydrogenase 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	774	0	T	C	97.4%	116.1 / ‑4.3	38	T146T (ACT→ACC)	thrA	fused aspartate kinase/homoserine dehydrogenase 1
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base C (28/9);  total (29/9)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.57e-01

GCCGAACGTATTTTTGCCGAACTTTTGACGGGACTCGCCGCCGCCCAGCCGGGGTTCCCGCTGGCGCAATTGAAAACTTTCGTCGATCAGGAATTTGCCCAAATAAAACATGTCCTGCATGGCATTAGTTTGTTGGGGCAGTGCCCGGATAGCATCAACGCTGCGCTGATTTGCCGTGGCGAGAAAATGTCGATCGCCATTATGGCCGGCGTATTAGAAGCGCGCGGTCACAACGTTACTGTTATCGATCCGGTCGAAAAACTGCTGGCAGTGGGGCATTACCTCGAATCTACCGTCGATATTGCTGAGTCCACCCGCCGTATTGCGGCAAGCCGCATTCCGGCTGATCACATGGTGCTGATGGCAGGTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTGCTTGGACGCAACGGTTCCGACTACTCTGCTGCGGTGCTGGCTGCCTGTTTACGCGCCGATTGTTGCGAGATTTGGACGGAC  >  NC_000913/535‑1020                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                       gcccaaCGTATTTTTTCCGAACTTTTGACGGGACTCGCCGCCGCCCAGCCGGGATTCCCGCTGGCGCAATTGAAAACTTTCGTCGATCAGGAATTTGCCCAAATAAAACATGTCCTGCATGGCATTAGTTTGTTGGGGCAGTGCCCGGATAGCATCAACGCTGCGCTGATTTGCCGTGGCGAGAAAATGTCGATCGCCATTATGGCCGGCGTATTAGAAGCGCGCGGTCACAACGTTACCGTTATCGATcc                                                                                                                                                                                                                                             <  2:136239/247‑1 (MQ=255)  ccGAACGTATTTTTGCCGACCTTTGGACGGGACTGGCCGCCTCCCAGCCGTGCGTCCAGCTGGCGCAATTGAAAACTTTCGTCGATCAGGAATTTGCCCAAATAAAACATGGCCTGCATGGCATTAGTTTGTTGGGGCAGTGCCCGGATAGCATCAACGCTGCGCTGATTTGCCGTTGCGAGAAAAGGTCGATCGCCATTATGGCCGGCGTATTAGAAGCGCGCGGTCACAACGTTACCGTTATCGATCCg                                                                                                                                                                                                                                            <  2:384585/251‑1 (MQ=255)    gAACGTCTTTTTGCCGAACTTTTGACGGGACTCGCCGCCGCCCAGCCGGGATTCCCGCTGGCGCCATTGAAAACTTTCGTCGATCAGGAATTTACCCAAATAAAACATGTCCTGCATGGCATTAGTTTGTTGGGGCAGTGCCCGGATAGCATCAACGCTGCGCTGATTTGCCGTGGCGAGAAAATGTCGATCGCCCTTATGGCCGGCGTATTAGAAGCGCGCGGTCACACCGTTACCGTTATCGATCCggg                                                                                                                                                                                                                                          >  2:433532/1‑250 (MQ=255)      aCGTATTTTTGCCGAACTTTTGACGGGACTCGCCGCCGCCCAGCCGGGATTCCCGCTGGCGCAATTGAAAACTTTCGTCGATCAGGAATTTGCCCAAATAAAACATGTCCTGCATGGCATTAGTTTGTTGGGGCAGTGCCCGGATAGCATCAACGCTGCGCTGATTTGCCGTGGCGAGAAAATGTCGATCGCCATTATGGCCGGCGTATTAGAAGCGCGCGGTCACAACGTTACCGTTATCGATCCGGTCg                                                                                                                                                                                                                                        <  1:310703/251‑1 (MQ=255)               tGCCGAACTTTTGACGGGACTCGCCGCCGCCCAGCCGGGATTCCCGCTGGCGCAATTGAAAACTTTCGTCGATCAGGAATTTGCCCAAATAAAACATGTCCTGCATGGCATTAGTTTGTTGGGGCAGTGCCCGGATAGCATCAACGCTGCGCTGATTTGCCGTGGCGAGAAAATGTCGATCGCCATTATGGCCGGCGTATTAGAAGCGCGCGGTCACAACGTTACCGTTATCGATCCGGTCGAAAAActg                                                                                                                                                                                                                                <  1:360430/250‑1 (MQ=255)                  cGAACTTTTGACGGGACTCGCCGCCGCCCAGCCGGGATTCCCGCTGGCGCAATTGAAAACTTTCGTCGATCAGGAATTTGCCCAAATAAACCATGTCCTGCATGGCATTAGTTTGTTGGGGCAGTGCCCGGATAGCATCAACGCTGCGCTGATTTGCCGTGGCGAGAAAATGTCGATCGCCATTATGGCCGGCGTATTAGAAGCGCGCGGTCACAACGTTACCGTTATCGATCCGGTCGAAAAACTGCTgg                                                                                                                                                                                                                            >  1:215785/1‑251 (MQ=255)                    aaCTTTTGACGGGACTCGCCGCCGCCCAGCCGGGATTCCCGCTGGCGCAATTGAAAACTTTCGTCGATCAGGAATTTGCCCAAATAAAACATGTCCTGCATGGCATTAGTTTGTTGGGGCAGTGCCCGGATAGCATCAACGCTGCGCTGATTTGCCGTGGCGAGAAAATGTCGATCGCCATTATGGCCGGCGTCTTAGAAGCGCGCGGTCACAACGTTACCGTTATCGATCCGGTCGAAAAACTGCTGGc                                                                                                                                                                                                                           >  1:131156/1‑250 (MQ=255)                                 aCTCGCCGCCGCCCAGCCGGGATTCCCGCTGGCGCAATTGAAAACTTTCGTCGATCAGGAATTTGCCCAAATAAAACATGTCCTGCATGGCATTAGTTTGTTGGGGCAGTGCCCGGATAGCATCAACGCTGCGCTGATTTGCCGTGGCGAGAAAATGTCGATCGCCATTATGGCCGGCGTATTAGAAGCGCGCGGTCACAACGTTACCGTTATCGATCCGGTCGAAAAACTGCTGGCAGTGGGGCATTAcc                                                                                                                                                                                                             <  1:348599/251‑1 (MQ=255)                                      ccgccgccCAGCCGGAATTCCCGCTGGCGCAATTGCAAACTTTCGTCGATCAGGACTTTGCCCAAATCAACAATGTCCTGCATGGCATTAGTTTGTTGGGGCAGTGCCCGGATCGCATCAACGCTGCGCTGCTTTGCCGTGGCGAGAAAATGTCGATCGCCATTATGGCCGGCGTATTAGACGCGAGCGGGCACAACGTTACCGTTATCGATCCGGTCGAAAAACTTCTGGCAGTGGGGCATTACCTCGa                                                                                                                                                                                                         >  2:66342/1‑250 (MQ=255)                                       cgcagccCCGCCGGGATTCCCGCCGGCCCAATTGAAAACTTTCGTCGATCAGGAAGTTGCACAAATAAAACATGTCCTGCATGGCAGTAGTTTGTGGGGGCAGGGCCCGGATAGCATCAACGCTGCGCTGATTTGCCGTGGCGAGAAAATGTCGATCGCCATTATGGCCGGCGTATTAGAAGCGCGCGGTCACAACGTTACCGTTATCGAGCCGGTCGAAAAACTGCTGGCAGTGGGGCATTCCCTCGAAt                                                                                                                                                                                                       <  2:327292/247‑1 (MQ=255)                                        gccgccCATCCGGGATTCCCGCTGGCGCAAGTGAAAACTTTCGGCGATCAGGAATTTGCCCAAATAAAACAGGTCCTGCATGGCATTAGTTTGTTGGGGCAGTGCCCGGATAGCATCAACGCTGCGCTGATTTGCCGTGGCGATAAAATGTCGATCGCCATTATGGCCGGCGTATTATAAGCGCGCGGTCACAACGTTACCATTATCGATCCGGTCGAAAAACTGCTGGCAGTGGGGCATTACCTCGAATc                                                                                                                                                                                                      <  1:332090/251‑1 (MQ=255)                                                gCCGGGATTCCCGCTGGCGCAATTGAAAACTTTCGTCGGTCAGGAATTTGCCCAAATAAAACATGTCCTGCATGGCATTAGTTTGTTGGGGCAGTGCCCGGATAGCATCAACGCTGCGCTGATTTTCCGTGGCGAGAAAATGTCGATCGCCATTATGGCCGGCGTATTAGAAGCGCGCGGTCACACAGTTGCCGTTATCGATCCGGTCGAAAAAATGCTGGCAGAGAGGCATTACCTCGAATCTACCGTcc                                                                                                                                                                                              >  2:336790/1‑250 (MQ=255)                                                  cGGGATTCCCGATGGCGCAATTGAAAACTTTCGTCGCTCAGGAATTTGACCAAATAAAACATGTCCTGCATGGCATTAGTTTGTTGGGGCAGTGCCCGGATAGCATCAACGCTGCGCTGATTTGCCGTGGCGAGAAAATGTCGATCGCCATTATGGCCGGCGTATTAGAATCGCGCGGTCACAACGTTACCGTTATCGCTCCGGTCGAAAAACTGCTGGCAGTGGGGCATTACCTCGAATCTACCGTCggt                                                                                                                                                                                            >  2:88003/1‑249 (MQ=255)                                                     gattCCCGCTGGCGCAATTGAAAACTTTCGTCGATCAGGAATTTGCCCAAATAAAACATGTCCTGCATGGCATTAGTTTGTTGGGGCAGTGCCCGGATAGCATCAACGCTGCGCTGATTTGCCGTGGCGAGAAAATGTCGATCGCCATTATGGCCGGCGTATTAGAAGCGCGCGGTCACAACGTTACCGTTATCGATCCGGTCGAAAAACTGCTGGCAGTGGGGCATTACCTCGAATCTACCGTCGATAtt                                                                                                                                                                                         >  2:244479/3‑251 (MQ=255)                                                         cccGCTGGCGCAATTGAAAACTTTCGTCGATCAGGAATTTGCCCAAATAAAACATGTCCTGCATGGCATTAGTTTGTTGGGGCAGTGCCCGGATAGCATCAACGCTGCGCTGATTTGCCGTGGCGAGAAAATGTCGATCGCCATTATGGCCGGCGTATTAGAAGCGCGCGGTCACAACGTTACCGTTATCGATCCGGTCGAAAAACTGCTGGCAGTGGGGCATTACCTCGAATCTACCGTCGATATTGCt                                                                                                                                                                                      <  1:202978/250‑1 (MQ=255)                                                            gCTGGCGCAATTGAAAACTTTCGTCGATCAGGAATTTGCCCAAATAAAACATGTCCTGCATGGCATTAGTTTGTTGGGGCAGTGCCCGGATAGCATCAACGCTGCGCTGATTTGCCGTGGCGAGAAAATGTCGATCGCCATTATGGCCGGCGTATTAGAAGCGCGCGGTCACAACGTTACCGTTATCGATCCGGTCGAAAAACTGCTGGCAGTGGGGCATTACCTCGAATCTACCGTCGATATTGCTAAGt                                                                                                                                                                                  >  2:111699/1‑251 (MQ=255)                                                               ggCGCAATTGAAAACTTTCGTCGATCAGGAATTTGCCCAAATAAAACATGTCCTGCATGGCATTAGTTTGTTGGGGCAGTGCCCGGATAGCATCAACGCTGCGCTGATTTGCCGTGGCGAGAAAATGTCGATCGCCATTATGGCCGGCGTATTAGAAGCGCGCGGTCACAACGTTACCGTTATCGATCCGGTCGAAAAACTGCTGGCAGTGGGGCATTACCTCGAATCTACCGTCGATATTGCTGCGTcc                                                                                                                                                                                >  1:54687/1‑250 (MQ=255)                                                                     aTTGAAAACTTTCGTCGATCAGGAATTTGCCCAAATAAAACATGTCCTGCATGGCATTAGTTTGTTGGGGCAGTGCCCGGATAGCATCAACGCTGCGCTGATTTGCCGTGGCGAGAAAATGTCGATCGCCATTATGGCCGGCGTATTAGAAGCGCGCGGTCACAACGTTACCGTTATCGATCCGGTCGAAAAACTGCTGGCAGTGGGGCATTACCTCGAATCTACCGTCGATATTGCTGAGTCCACccgcc                                                                                                                                                                         <  1:292692/251‑1 (MQ=255)                                                                       tGAAAACTTTCGTCGATCAGGAATTTGCCCAAATAAAACATGTCCTGCATGGCATTAGTTTGTTGGGGCAGTGCCCGGATAGCATCAACGCTGCGCTGATTTGCCGTGGCGAGAAAATGTCGATCGCCATTATGGCCGGCGTATTAGAAGCGCGCGGTCACAACGTTACCGTTATCGATCCGGTCGAAAAACTGCTGGCAGTGGGGCATTACCTCGAATCTACCGTCGATATTGCTGAGTCCACCCGCCGt                                                                                                                                                                       >  1:118772/1‑251 (MQ=255)                                                                         aaaaCTTTCGTCGATCAGGAATTTGCCCAAATAAAACATGTCCTGCATGGCATTAGTTTGTTGGGGCAGTGCCCGGATAGCATCAACGCTGCGCTGATTTGCCGTGGCGAGAAAATGTCGATCGCCATTATGGCCGGCGTATTAGAAGCGCGCGGTCACAACGTTACCGTTATCGATCCGGTCGAAAAACTGCTGGCAGTGGGGCATTACCTCGAATCTACCGTCGATATTGCTGAGTCCACCCGCCGTAt                                                                                                                                                                     >  1:295862/1‑251 (MQ=255)                                                                                      aTCAGGAATTTGCCCAAATAAAACATGTCCTGCATGGCATTAGTTTGTTGGGGCAGTGCCCGGATAGCATCAACGCTGCGCTGATTTGCCGTGGCGAGAAAATGTCGATCGCCATTATGGCCGGCGTATTAGAAGCGCGCGGTCACAACGTTACCGTTATCGATCCGGTCGAAAAACTGCTGGCAGTGGGGCATTACCTCGAATCTACCGTCGATATTGCTGAGTCCACCCGCCGACTTGCGGCAAGTCGc                                                                                                                                                        >  2:136580/1‑251 (MQ=255)                                                                                                   ccAAATAAAACATGTCCTGCATGGCATTAGTTTGTTGGGGCAGTGCCCGGATAGCATCAACGCTGCGCTGATTTGCCGTGGCGAGAAAATGTCGATCGCCATTATGGCCGGCGTATTAGAAGCGCGCGGTCACAACGTTACCGTTATCGATCCGGTCGAAAAACTGCTGGCAGTGGGGCATTACCTCGAATCTACCGTCGATATTGCTGAGTCCACCCGCCGTATTGCGGCAAGTCGCATTCCGGCTGATc                                                                                                                                           >  1:253073/1‑251 (MQ=255)                                                                                                         aaaaCATGTCCTGCACGGCCTTAGCTTGTTGGGGCAGTGCCCGAATAGCATCAACGCTGCGATGATTTGACGTGGCGAGAAAATGTCGATCGACATTATGGCCGGCGTATTAGCAGCGCGCGGTCACAACGCTCCCGTTATCGATTCGGTCGAAAAACTGCTGGCAGTGGGGCATTACCTCGAATCTACCGTCGCTATACCTGAGTCCACCCGCCGTATTGCGTCAAGTCGCATTCCGGCTGATccgatgg                                                                                                                                     >  2:233208/1‑245 (MQ=255)                                                                                                           aaCATGTCCTGCATGGCATTAGTTTGTTGGGGCAGTGCCCGGATAGCATCAACGCTGCGCTGATTTGCCGTGGCGCGAAAATGTCGATCGCCATTATGGCCGGCGTATTAGGAGCGCGCGGTCACAACGTTACCGTTATCGATCCGGTCGAAAAACTGCTGGCAGTGGGGCTTTACCTCGCATCTACCGCCGATTTTGCTGAGTCCACCCGCCGTATTGCGGCAAGTCGCATTCCGGCTGATCAAATgttg                                                                                                                                   >  2:432545/1‑248 (MQ=255)                                                                                                                                                               cgctgcgctgATTTGCCGTGGCGAGAAAATGTCGGTCGCCATTATGGCCGGCGTATTAGAGGCGCGCGGTCACCACGTTACCGTTATCGATCCGGTCGAAAAACTGCTGGCAGTGGGGCATTACATCGCATCTAACGTCGATATTGCTGAGTCCACCCGCCGTATTGCGGCAAGTAGCCTTCCGGCTGATCACATGGTGCAGATGGCAGGTTTCAACGCCGGGAATGAAAAAgggggacaggggggagttt                                                                               >  2:279987/1‑234 (MQ=255)                                                                                                                                                                             gCCGTGGCGAGAAAATGTCGATCGCCATTATGTCCGGCGTATTAGAAGCGCGCGGTCACAACGTTACCGTTATCGATCCGGTCGAAAAACTGCTGGCCGTGGGGCATTACCTCGAATCTACCGTCGATATTGCTGAGTCCACCCGCCGTATTGCGGCAAGTCGCATTCCGGCTGATCACATGGGGCTGATGGCAGGTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGGACTTGGACGCAACGGTTcc                                                                 >  1:35869/1‑251 (MQ=255)                                                                                                                                                                                 tcgCGAGAAAATGTCGATCGCCATTATGGCCGGCGTATTAGAAGCGCGCGGTCACAACGTTACCGTTATCGATCCGGTCGAAAAACTGCTGGCAGTGGGGCATTACCTCGAATCTACCGTCGATATTGCTGAGTCCACCCGCCGTATTGCGGCAAGTCGCATTCCGGCTGATCACATGGTGCTGCTGGCAGGTTTcccc                                                                                                                 >  2:102151/3‑196 (MQ=255)                                                                                                                                                                                       gaAAATGTCGCCCGCCATTCTAGCCGGCGTCTTAGAAGGGCGCGGTCACAAAGTTACCGTTATCGATCCGGTCGAAAAACTGCTGGCCGTGGGGCATTACCTCGAATCTACCGTCGATATTGCTGAGTCCCCCCGCCGTATTGCGGCAAGACGCCTTCCGGc                                                                                                                                                >  2:318504/1‑162 (MQ=255)                                                                                                                                                                                        aaaaTGTCGATCGCCATTATGGCCGGCGTATTAGAAGCGCGCGGTCACAACGTTACCGTTATCGATCCGGTCGAAAAACTGCTGGCAGTGGGGCATTACCTCGAATCTACCGTCGATATTGCTGAGTCCACCCGCCGTATTGCGGCAAGTCGCATTCCGGCTGATCACATGGTGCTGATGGCAGGTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTACTTGGACGCAACGGTTCCGACTActccgc                                                      >  1:189956/1‑248 (MQ=255)                                                                                                                                                                                                   cGCCATTATGGCCGGCGGATTAGAAGCGCGCGGTCACAACGTTACCGTTATCGATCCGGTCGAAAAACTGCTGGCAGTGGGGCATTACCTCGAATCTACCGTCGATATTGCTGAGTCCACCCGCCGTATTGCGGCAAGTCGCATTCCGGCTGATCACATGGTGCTGATGGCAGGTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTACTTGGACGCAACGGTTCCGCCTActccccggcggtgccgg                                           >  2:63015/1‑237 (MQ=255)                                                                                                                                                                                                     ccATTAAGGCCGGCGTATTAGACGCGCGCGGTCACAACGTTACCGTTATCGATCCGGTCGAAAAACTGCTGGCAGTGGGGCATTACCTCGAATCTACCGTCGTTATTGCTGAGTCCACCCGCCGTATTGCGGCAAGTCGCATTCCGGCTGATCACATGGTGCTGATGGCAGGATTCACTGCCGGTAATGACAAAGGCGACCTGGTGTTACTTTGACGCAACGGTTCCGACTACTCCGCGGCGGTgccggct                                         >  1:302491/1‑251 (MQ=255)                                                                                                                                                                                                                   gTATTAGAAGCGCGCGGTCACAACGTTACCGTTATCGATCCGGTCGAAAAACTGCTGGCAGTGGGGCATTACCTCGAATCTACCGTCGATATTGCTGAGTCCACCCGCCGTATTGCGGCAAGTCGCATTCCGGCTGATCACATGGTGCTGATGGCAGGTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTCCTTGGACGCAACGGTTCCGACTACTCCGCGGCGGTGCTGGATGCCTGTTTACgcgc                           >  1:21671/1‑251 (MQ=255)                                                                                                                                                                                                                          aaGCGCGAGGTCACAACGTTACCGTTATCGATCCGGTCGAAAAACTGCTGGCAGTGGGACATTACCTCGAATCTACCGTCGAAATTGCTGAGTCCCCCCGCCGTATTGCGCCAAGTCGCATTCCGGCTGCTCACATGGTGCTGATGGCAGGTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTACTTGGACGCAACGGTTCCGCCTACTACGCGGCGGAGCTGGCTGCCAGTTTATGCGACGActgt                    >  1:313276/1‑251 (MQ=255)                                                                                                                                                                                                                                  ggTCACAACGTTACCGTTATCGATCCGGTCGAAAAACTGCTGGCAGTGGGGCATTACCTCGAATCTACCGTCGATATTGCTGAGTCCACCCGCCGTATTGCGGCAAGTCGCATTCCGGCTGATCACATGGTGCTGATGGCAGGTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTACTTGGACGCAACGGTTCCGACTACTCCGCGGCGGTGGTGGGTGCCCTTTTTCGCGCCCGTTGTTGCgaggt            >  2:175749/1‑249 (MQ=255)                                                                                                                                                                                                                                      acaACGTTACTGTTATCGATCCGGTCGAAAAACTGCTGGCAGTGGGGCATTACCTCGAATCTACCGTCGATATTGCTGAGTACACCCGCCGTATTGCGGCAAGCCGCATTCCGGCTGATCACATGGTGCTGATGGCAGGTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTGCTTGGACGCAACGGTTCCGACTAATCCGCTGCGGTGCTGGCTGCCTGTTTACGCGCCGATTGTTGctagcttttg        >  2:142420/1‑242 (MQ=255)                                                                                                                                                                                                                                      acaACGTTACCGTTATCGATCCGGTCGAAAAACTGCTGGCAGTGGGGCATTACCTCGAATCTACCGTCGATATTGCTGAGTCCACCCGCCGTATTGCGGCAAGTCGCATTCCGGCTGATCACATGGTGCTGATGGCAGGTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTACTTGGACGCAACGGTTCCGACTACTCCGCGGCGGTGCTGGCTGCCTGTTTACGCGCCGATTGTTGCGAGATTTgg        >  1:318425/1‑251 (MQ=255)                                                                                                                                                                                                                                           gTTACCGTTATCGATCCGGTCGAAAAACTGCTGGCAGTGGGGCATTACCTCGAATCTACCGTCGATATTGCTGAGTCCACCCGCCGTATTGCGGCAAGTCGCATTCCGGCTGATCACATGGTGCTGATGGCAGGTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTACTTGGACGCAACGGTTCCGACTACTCCGCGGCGGTGCTGGCTGCCTGTTTACGCGCCGATTGTTGCGAGATTTggacggg   >  1:183809/1‑250 (MQ=255)                                                                                                                                                                                                                                            ttACCGTTATCGATCCGGTCGAAAAACTGCTGGCAGTGGGGCATTACCTCGAATCTACCGTCGATATTGCTGAGTCCAACCGCCGTATTGCGGCAAGTCGCATTCCGGCTGATCACATGGTGCTGCTGGCAGGTTTCACCGCCGGTCATGAAAAAGGCGAACTGGTGGTACTTGGACGCAACGGTTCCGACTACTCAGCGGCGGGTCTGGCTGCCTGTTTTAGCGCCGGttgttggcggtattgtgaggac  >  2:466144/1‑235 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                       GCCGAACGTATTTTTGCCGAACTTTTGACGGGACTCGCCGCCGCCCAGCCGGGGTTCCCGCTGGCGCAATTGAAAACTTTCGTCGATCAGGAATTTGCCCAAATAAAACATGTCCTGCATGGCATTAGTTTGTTGGGGCAGTGCCCGGATAGCATCAACGCTGCGCTGATTTGCCGTGGCGAGAAAATGTCGATCGCCATTATGGCCGGCGTATTAGAAGCGCGCGGTCACAACGTTACTGTTATCGATCCGGTCGAAAAACTGCTGGCAGTGGGGCATTACCTCGAATCTACCGTCGATATTGCTGAGTCCACCCGCCGTATTGCGGCAAGCCGCATTCCGGCTGATCACATGGTGCTGATGGCAGGTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTGCTTGGACGCAACGGTTCCGACTACTCTGCTGCGGTGCTGGCTGCCTGTTTACGCGCCGATTGTTGCGAGATTTGGACGGAC  >  NC_000913/535‑1020

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑