Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,894,839 T→C L12P (CTC→CCC)  pabB → aminodeoxychorismate synthase subunit 1

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,894,8390TC96.3% 82.0 / ‑4.1 28L12P (CTC→CCC) pabBaminodeoxychorismate synthase subunit 1
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base C (14/12);  total (16/12)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.20e-01

CGGAGTGGTTGGCACGCAGCTCTTCTGCCACCAGCGCGATTGCCTGGCCGCTGCTCATCCCTTGTGCCATCAGTTCCTGGATCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACTCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGCCACCTGCCGTGGGCGATGCTTTTACACTCCGGCTATGCCGATCATCCGTATAGCCGCTTTGATATTGTGGTCGCCGAGCCGATTTGCAC‑TTTAACCAC‑TTTCGGTAAAGAAACCGTTGTTAGTGAAAGCGAAAAACGCACAACGACCACTGATGACCCGCTACAGGTGCTCCAGCAGGTGCTGGATCGCGCAGACATTC  >  NC_000913/1894593‑1895081
                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                    
cGGAGTGGTTGGCACGCAGCTCTTCTGCCACCAGCGCGATTGCCTGGCCGCTGCTCATCCCTTGTGCCATCAGTTCCTGGATCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACCCTgg                                                                                                                                                                                                                                                  <  2:229000/251‑1 (MQ=255)
  gAGTGGTTGGCACGCAGCTCTTCTGCCACCAGCGCGATTGCCTGGCCGCTGCTCATCCCTTGTGCCATCAGTTCCTGGATCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACCCTGGCg                                                                                                                                                                                                                                                <  2:180988/251‑1 (MQ=255)
                                   gcgATTGCCTGGCCGCTGCTCATCCCTTGTGCCATCAGTTCCTGGATCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACCCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCtt                                                                                                                                                                                                               >  1:188713/1‑251 (MQ=255)
                                                   tgctCATCCCTTGTGCCATCAGTTCCTGGATCCGCTCGCCAGCTTTTTGCTGCTGTTCATGGGCGAGTGAAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACCCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGCCACCTGCCGTgg                                                                                                                                                                                               >  1:11580/1‑251 (MQ=255)
                                                    gctCATCCCTTGTGCCATCAGTTCCTGGATCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTTCCCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGCCACCTGCCGTg                                                                                                                                                                                                <  1:296903/249‑1 (MQ=255)
                                                     ctCATCCCTTGTGCCATCAGTTCCTGGATCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACCCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGCCACCTGCCGTGGGc                                                                                                                                                                                             >  1:363670/1‑251 (MQ=255)
                                                                                       cGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACCCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGCCACCTGCCGTGGGCGATGCTTTTACACTCCGGCTATGCCGATCATcc                                                                                                                                                            <  1:49595/250‑1 (MQ=255)
                                                                                                      gctgTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTAACCCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACCCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGCCACCTGCCGTGGGCGATGCTTTTACACTCCGGCTATGCCGATCATCCGTATAGCCGCTTTGa                                                                                                                                             <  2:194460/250‑1 (MQ=255)
                                                                                                        tgTTCATGGGAGAGTGAAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCTGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACCCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGCCACCTGCCGTGGGCGATGCTTTTACACTCCGGCTATGCCGATCATCCGTATAGCCGCTTTGatat                                                                                                                                          >  1:355188/1‑251 (MQ=255)
                                                                                                                              aaCCTGCAAACATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACCCTGGCGTCAGGCCGCAGCTGAATTTTATTTCTCCCGCTTAAGCCACCTGCCGTGGGCGTTGCTTTTACACTCCGGCTATGCCGAACATCCGTATAGCCGCTTTGATATTGTGGTCGCCGAGCCGATTTGc                                                                                                                    >  2:238815/1‑251 (MQ=255)
                                                                                                                                ccTGCAAACATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACCCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGCCACCTGCCGTGGGCGATGCTTTTACACTCCGGCTATGCCGATCATCCGTATAGCCGCTTTGATATTGTGGTCGCCGAGCCGATTTGCAc                                                                                                                  <  1:78500/251‑1 (MQ=255)
                                                                                                                                   gCAAACATTGTTAACCCCTGCTAAATGGTTGGCGCTATTTATTTCATGCTACCCGGCACAGAGCCAGTATGGTCAGGACTGATGAAGACGTTATCTCCCGCTGTTATTACTTTACCCTGGCGTCAGGAGGCCGCTGAATTTTATTTCTCCCGCTTAAGCCACCGGCCGTGGGCGATGCTTTTACACTCCGGCTATGCCGATCATCCGTATAGCCGCTTTGATATTGTGGTCGCCGAGCCGATTTGCAC‑t                                                                                                                <  2:484965/249‑1 (MQ=255)
                                                                                                                                    cAAACATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACCCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGCCACCTGCCGTGGGCGATGCTTTTACACTCCGGCTATGCCGATCATCCGTATAGCCGCTTTGATATTGTGGTCGCCGAGCCGATTTGCCC‑ttt                                                                                                              >  2:159533/1‑250 (MQ=255)
                                                                                                                                             gtaacgccggttAAATTGTTGGCGTTAATTATTTATTCCTACCGGGCACATTGCAGGTAGAGGCAGGACTGATGAAGCCGTTATCTCCCCCGTTGATTACTTCCCCCGGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGTTTAAGCCTCCTGCCGTGGGCGATGCTTTTACACTCCGGCTATGCCGATCATCCGTATAGCCGCTTTGATATTGTTGTCGCCGAGCCGATTTGCAC‑TTTAACCTC‑ttt                                                                                                    <  2:285078/239‑1 (MQ=255)
                                                                                                                                                  tCCTGCTAAATTGTTGGGGCTAATTTGTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGTTTACTTTACCCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGCCACCTGCCGTGGGCGATGCTTTTACACTCCGGCTATGCCGATCATCCGTATAGCCGCTTTGATATTGTGGTCGCCGAGCCGATTTGCAC‑TTTAACCAC‑TTTCGGTa                                                                                               <  1:146741/250‑1 (MQ=255)
                                                                                                                                                      gCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACCCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGCCACCTGCCGTGGGCGATGCTTTTACACTCCGGCTATGCCGATCATCCGTATAGCCGCTTTGATATTGTGGTCGCCGAGCCGATTTGCAC‑TTTAACCAC‑TTTCGGTAAAGa                                                                                           >  1:422162/1‑250 (MQ=255)
                                                                                                                                                        aaaaTTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGGAGAGCCAGGACTGATGAAGACGTTAACTCCCGCTGGGATTCCTTTCCCCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGCCACCTGCCGTGGGCGATGCTTTTACACTCCGGCTATGCCGATCATCCGTATAGCCGCTTTGATATTGTGGTCGCCGAGCCGATTTGCAC‑TTTAACCAC‑TTTCGGTAAAGAAAc                                                                                        <  2:226091/250‑1 (MQ=255)
                                                                                                                                                         acgttgttgGCGCTAATTATTTCAAGATACCCGGAACATAGCCAGTAGTGTCAGGACTGCTGAAGACGTTATCTCCCGCTGTGATTACTTTACACTGACGTAAGGACGCCGCTGCATTTTATTTCTCCCGCTTAAGCCACCTGCAGTGGGCGATGATTTTACACCCCTGCTATGCAGATCATCCGTATAGCCGCTTTGATATTGTGGTCGACATGCAGATTTG‑ACATTTAACCAA‑TTTAGGTAAAGCAAc                                                                                        >  2:285607/4‑250 (MQ=255)
                                                                                                                                                                             ttCATGCTCCCCGGCTCCTAGCCAGCAGAGTCAGGCCTGATAAAGACGTTCTCTCCCGCTGTGATTACTTTACCCTGGCGTCAGTACGACGCTGAATTTTCTTTCTCCCGCTTAAGCCACCTGCCGTGGGCGATGCTTTTACACTCCGGCTATGCCGATCATCCCTATAGCCGCTTTGATATTGTAGCCGCCAAACCGATTTCCAC‑TTTAAC‑ACATTTCGGTAAAGAAACCGTTGATAGTGAAAGCGaaa                                                                    >  2:347284/1‑250 (MQ=255)
                                                                                                                                                                                                 gCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACCCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGCCACCTGCCGTGGGCGATGCTTTTACACTCCGGCTATGCCGATCATCCGTATAGCCGCTTTGATATTGTGGTCGCCGAGCCGATTTGCAC‑TTTAACCAC‑TTTCGGTAAAGAAACCGTTGTTAGTGAAAGCGAAAAACGCACAACGACCACtgatg                                               >  1:239659/1‑251 (MQ=255)
                                                                                                                                                                                                    aGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACCCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGCCACCTGCCGTGGGCGATGCTTTTACACTCCGGCTATGCCGATCATCCGTATAGCCGCTTTGATATTGTGGTCGCCGAGCCGATTTGCAC‑TTTAACCAC‑TTTCGGTAAAGAAACCGTTGTTAGTGAAAGCGAAAAACGCACAACGACCACTGATGAcc                                            <  1:463531/251‑1 (MQ=255)
                                                                                                                                                                                                    aGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACCCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGCCACCTGCCGTGGGCGATGCTTTTACACTCCGGCTATGCCGATCATCCGTATAGCCGCTTTGATATTGTGGTCGCCGAGCCGATTTGCAC‑TTTAACCAC‑TTTCGGTAAAGAAACCGTTGTTAGTGAAAGCGAAAAACGCACAACGACCACTGATGAcc                                            >  2:82809/1‑251 (MQ=255)
                                                                                                                                                                                                                   gatgaAGACGTTATCTCCCGCTGTGATTACTTTACTCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGCCACCTGCCGTGGGCGATGCTTTTACACTCCGGCTATGCCGATCATCCGTATAGCCGCTTTGATATTGTGGTCGCCGAGCCGATTTGCAC‑TTTAACCAC‑TTTCGGTAAAGGAACCGTTGTTAGTGAAAGCGAAAAACGCACAACGACCACTGATGACCAGCTACAGGTGCTcc                             >  1:153641/1‑251 (MQ=255)
                                                                                                                                                                                                                      gaAGACGTTATCTCCCGCTGTGATTACTTTACCCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGCCACCTGCCGTGGGCGATGCTTTTACACTCCGGCTATGCCGATCATCCGTATAGCCGCTTTGATATTGTGGTCGCCGCTCCGATTTGCAC‑TTTACCCAC‑TTTCGGTAAAGAACCCGTTGTTAGTGAAAGCGAAAAACGCACAACGACCACTGCTGACACGCTACAGGTGCTCcagc                          >  2:31606/1‑251 (MQ=255)
                                                                                                                                                                                                                      gaAGACGTTATCTCCCGCTGTGATTACTTTACCCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGCCACCTGCCGTGGGCGATGCTTTTACACTCCGGCTATGCCGATCATCCGTATAGCCGCTTTGATATTGTGGTCGCCGAGCCGATTTGCAC‑TTTAACCAC‑TTTCGGTAAAGAAACCGTTGTTAGTGAAAGCGAAAAACGCACAACGACCCCTGATGACCCGCTACAGGTGCTCcagc                          >  2:183891/1‑251 (MQ=255)
                                                                                                                                                                                                                                  tcCCGCTGTGATTACTTTACCCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGCCACCTGCCGTGGGCGATGCTTTTACACTCCGGCTATGCCGATCATCCGTATAGCCGCTTTGATATTGTGGTCGCCGAGCCGATTTGCAC‑TTTAACCAC‑TTTCGGTAAAGAAACCGTTGTTAGTGAAAGCGAAAAACGCACAACGACCACTGATGACCCGCTACAGTTGCTCCAGCAGGTGCTGGATc              >  1:89046/1‑251 (MQ=255)
                                                                                                                                                                                                                                   cccGCTGTGATTACTTTACCCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGCCACCTGCCGTGGGCGATGCTTTTACACTCCGGCTATGCCGATCATCCGTATAGCCGCTTTGATATTGTGGTCGCCGAGCCGATTTGCAC‑TTTAACCAC‑TTTCGGTAAAGAAACCGTTGTTAGTGAAAGCGAAAAACGCACAACGACCACTGATGACCCGCTACAGGAGCTCCAGCAGGTGCTGGATcg             >  2:482275/1‑251 (MQ=255)
                                                                                                                                                                                                                                   cccGCTGTGATTACTTTACCCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGCCACCTGCCGGGGGCGATGCTTTTACACTCCGGCTATGCCGATCATCCGTATAGCCGCTTTGATATTGTGGTCGCCGAGCCGATTTGCAC‑TTTAACCAC‑TTTCGGTAAAGAAACCGTTGTTAGTGAAAGCGAAAAACGCACAACGACCACTGATGACCCGCTACAGGTGCTCCAGCAGGTGCTgg                 <  1:238815/247‑1 (MQ=255)
                                                                                                                                                                                                                                               attttaccCTGGCGCCAGGCCGCCCTTGTATTTTATTTCTCCCGCTTAAGCCACCGGCCGTGGGCGATGCTTTTACACTCCGGCTATGCCGATCATCCGTATAGCCGCTTTGATATTGTGGTCGCCGAGCCGATTTGCAC‑TTTAACCAC‑TTTCGGTAAAGAAACCGTTGTTAGTGAAAGCGAAAAACGCACAACGACCACTGATGACCCGCTACAGGTGCTCCAGCAGGTGCTGGATCGCGCAGACATTc  <  2:209190/248‑1 (MQ=255)
                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                    
CGGAGTGGTTGGCACGCAGCTCTTCTGCCACCAGCGCGATTGCCTGGCCGCTGCTCATCCCTTGTGCCATCAGTTCCTGGATCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACTCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGCCACCTGCCGTGGGCGATGCTTTTACACTCCGGCTATGCCGATCATCCGTATAGCCGCTTTGATATTGTGGTCGCCGAGCCGATTTGCAC‑TTTAACCAC‑TTTCGGTAAAGAAACCGTTGTTAGTGAAAGCGAAAAACGCACAACGACCACTGATGACCCGCTACAGGTGCTCCAGCAGGTGCTGGATCGCGCAGACATTC  >  NC_000913/1894593‑1895081

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑