Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,632,513	C→T	intergenic (‑228/‑377)	ydfJ ← / → ynfT	putative transporter YdfJ/protein YnfT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,632,513	0	C	T	100.0%	25.5 / NA	10	intergenic (‑228/‑377)	ydfJ/ynfT	putative transporter YdfJ/protein YnfT
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (6/4);  total (6/4)

AACTGGAAATCCATAATTCACAGGTGTTTTTTCCCATCCTGTGGTTTCCTTGGCGTTTTCTAGGTTTTTTCAGATAGTTGCATTTTTTTAAAAAGCATCCTAAGTTCGATCTCAGTGTCTATCTGGGGCCTATTTCTGTCCCATATATGCCCCAAAAAAACTCCCCAACAGATAAGTAGTTTTTTCATGGATTTATGCGTAAAATCAAGAACGGCTGGAAATCATTCAATACTCACACTATCGAAAAATTTACCAGCCAATCGCAGCACGTTCTTGCATAAGGTGTGT‑CTGCGGTTTTTCAACTATTCAGATACATCACTCCCATCACA‑TTCATTCCTCCGCATCAAAGGCATATAGGCTATATCACCTTGATATTTTTCTTTTTCAGATAAAAACTGTTATCTATGTATACTTTTAAACCCAATCCGTGTAGAGTCTCTACATAAGATAGTTTGCAGT  >  NC_000913/1632272‑1632730                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                            aaCTGGAAATCCATAATTCACAGGTGTTTTTTCCCATCCTGTGGTTTCCTTGGCGTTTTCTAGGTTTTTTCAGATAGTTGCATTTTTTTAAAAAGCATCCTAAGTTCGATCTCAGTGTCTATCTGGGGCCTATTTCTGTCCCATATATGCCCCAAAAAAACTCCCCAACAGATAAGTAGTTTTTTCATGGATTTATGCGTAAAATCAAGAACGGATGGAAATCATTCAATACTCACACTATTGAAAAAttt                                                                                                                                                                                                                    >  1:74191/1‑251 (MQ=255)   cTGGAAATCCATAATTCACAGGTGTTTTTTCCCATCCTGTGGTTTCCTTGGCGTTTTCTAGGTTTTTTCAGATAGTTGCATTTTTTTAAAAAGCATCCTAAGTTCGATCTCAGTGTCTATCTGGGGCCTATTTCTGTCCCATATATGCCCCAAAAAAACTCCCCAACAGATAAGTAGTTTTTTCATGGATTTATGCGTAAAATCACGAACGGCGGGAAATCATTCAATACTCACACTCTTGAAAAATTTAc                                                                                                                                                                                                                  >  2:449224/1‑251 (MQ=255)     ggAAGTCCATAATTCACATGTGATTTTTCCCAACCTGTGGTTTCCTTGGCGTTTTCTAGGTTTTTTCAGATAGTTGCATTTTTTTAAAAAGCATCCTAAGTTCGATCTCAGTGTCTATCTTGGGCCTATTTCTGGCCCATATATGCCCCAAAAAAACTCCCCAACAGATAAGTAGTTTTTTCATGGATTTATGCGTAAAAGCAAAAACGGCAGGAAATCATTCAATACTCACTCTATTGAAAAATTTAc                                                                                                                                                                                                                  <  1:147751/249‑1 (MQ=255)                  tCACAGGTGTTTTTTCCCATCCTGTGGTTTCCTTGGCGTTTTCTAGGTTTTTTCAGATAGTTGCATTTTTTTAAAAAGCATCCTAAGTTCGATCTCAGTGTCTATCTGGGGCCTATTTCTGTCCCATATATGCCCCAAAAAAACTCCCCAACAGATAAGTAGTTTTTTCATGGATTTATGCGTAAAATCAAGAACGGCTGGAAATCATTCAATACTCACACTATTGAAAAATTTACCAGCCAATCgcagc                                                                                                                                                                                                    <  1:401136/250‑1 (MQ=255)                                      ccTGTGGTTTCCTTGGCGTTTTCTAGGTTTTTTCAGATAGTTGCATTTTTTTAAAAAGCATCCTAAGTTCGATCTCAGTGTCTATCTGGGGCCTATTTCTGTCCCATATATGCCCCAAAAAAACTCCCCAACAGATAAGTAGTTTTTTCATGGATTTATGCGTAAAATCAAGAACGGCTGGAAATCATTCAATACTCACACTATTGAAAAATTTACCAGCCAATCGCAGCACGTTCTTGCATAAGgtgtgt                                                                                                                                                                               >  1:132846/1‑251 (MQ=255)                                                                                                cATCCTAAGTTCGATCTCAGTGTCTATCTGGGGCCTATTTCTGTACCATATATGCCCCAAAAAACCTCCCCAACAGCTAAGTAGTTTTTTCATGGATTTATGCGTAAAATCAAGAACGGCTGGAAATCATTCAATACTCACACTATTGAAAACTATACCCGCCAATCACAGCACGTTCTTGCATAAGGTG‑GTGCTGCGGGTTTTCAACTATTCAAATACCTCACTCCCAT‑ACAATTCATTCCTCCGCaac                                                                                                                    >  2:52277/1‑248 (MQ=255)                                                                                                                                       tCTGTCCCATATATGCCCCAAAAAAACTCCCCAACAGATAAGTAGTTTTTTCATGGATTTATGCGTAAAATCAAGAACGGCTGGAAATCATTCAATACTCACACTATTGAAAAATTTACCAGCCAATCGCAGCACGTTCTTGCATAAGGTGTGT‑CTGCGGTTTTTCAACTATTCAGATACATCACTCCCATCACA‑TTCATTCCTCCGCATCAAAGGCATATAGGCTATATCACCTTGATAtttttcttttt                                                                            >  1:421687/1‑251 (MQ=255)                                                                                                                                                                     ccAACAGATAAGTAGTTTTTTCATGGATTTATGCGTAAAATCAAGAACGGCTGGAAATCATTCAATACTCACACTATTGAAAAATTTACCAGCCAATCGCAGCACGTTCTTGCATAAGGTGTGT‑CTGCGGTTTTTCAACTATTCAGATACATCACTCCCATCACA‑TTCATTCCTCCGCATCAAAGGCATATAGGCTATATCACCTTGATATTTTTCTTTTTCAGATAAAAACTGTTATCTATGTATACttt                                              <  2:380539/251‑1 (MQ=255)                                                                                                                                                                                          cATGGATTTATGCGTAAAATCAAGAACGGCTGGAAATCATTCAATACTCACACTATTGAAAAATTTACCAGCCAATCGCAGCACGTTCTTGCATAAGGTGTGT‑CTGCGGTTTTTCAACTATTCAGATACATCACTCCCATCACA‑TTCATTCCTCCGCATCAAAGGCATATAGGCTATATCACCTTGATATTTTTCTTTTTCAGATAAAAACTGTTATCTATGTATACTTTTAAACCCAATCCGTGTAGAGt                         >  1:360410/1‑251 (MQ=255)                                                                                                                                                                                                                 gAACGGCTGGAAATCACTCAATCCTCACACTATTGAAAAATTTACCAGCCAATCGCAGCACGTTCTTGCATAAGGTGTGT‑CTGCGGTTTTTCAACTATTCAGATACATCACTCCCATCACA‑TTCATTCCTTCGCATCAAAGGCATATAGGCTATATCACCTTGATATTTTTCTTTTTCAGATAAAAACTGTTATCTATGTATACTTTTAAACCCAATCCGTGTAGAGTCTCTACATAAGATAGTCTGCAGt  <  2:85054/251‑1 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                            AACTGGAAATCCATAATTCACAGGTGTTTTTTCCCATCCTGTGGTTTCCTTGGCGTTTTCTAGGTTTTTTCAGATAGTTGCATTTTTTTAAAAAGCATCCTAAGTTCGATCTCAGTGTCTATCTGGGGCCTATTTCTGTCCCATATATGCCCCAAAAAAACTCCCCAACAGATAAGTAGTTTTTTCATGGATTTATGCGTAAAATCAAGAACGGCTGGAAATCATTCAATACTCACACTATCGAAAAATTTACCAGCCAATCGCAGCACGTTCTTGCATAAGGTGTGT‑CTGCGGTTTTTCAACTATTCAGATACATCACTCCCATCACA‑TTCATTCCTCCGCATCAAAGGCATATAGGCTATATCACCTTGATATTTTTCTTTTTCAGATAAAAACTGTTATCTATGTATACTTTTAAACCCAATCCGTGTAGAGTCTCTACATAAGATAGTTTGCAGT  >  NC_000913/1632272‑1632730

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑