Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,341,542	G→A	A329T (GCG→ACG)	lapB →	lipopolysaccharide assembly protein B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,341,542	0	G	A	93.6%	124.7 / 0.4	47	A329T (GCG→ACG)	lapB	lipopolysaccharide assembly protein B
Reads supporting (aligned to +/- strand):  ref base G (1/2);  new base A (18/26);  total (19/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.78e-01

GGTCAGCGAAACGCTGGAAATGTTGCAAACCTGCTATCAGCAGTTGGGTAAAACTGCCGAATGGGCAGAATTCCTGCAGCGTGCGGTGGAAGAGAACACCGGTGCCGATGCTGAATTGATGCTTGCTGATATCATCGAAGCGCGCGACGGTAGTGAGGCCGCACAGGTCTATATTACGCGCCAGCTTCAGCGTCATCCGACCATGCGTGTGTTCCATAAGTTAATGGATTACCACTTAAATGAAGCGGAAGAAGGGCGTGCCAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAAGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCATACACCCTCTACTGGCATTGTCCGTCTTGTCGGGCCTGGTCAACCATTAAACCGATTCGCGGTCTTGATGGCCTGTAATTTTTAAAAAAAATCCGACTTTAGTTACAACATACTAATTATTAATGTTCCATTGTGCTC  >  NC_000913/1341298‑1341787                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                      gggcAGCGAAACGCTGGAAATGTTGCAAACCTGCTATCAGCAGTTGGGTAAAACTGCCGAATGGGCAGAATTCCTGCAGCGTGCGGTGGAAGAGAACACCGGTGCCGATGCTGAATTGATGCTTGCTGATATCATCGAAGCGCGCGACGGTAGTGAGGCCGCACAGGTCTATATTACGCGCCAGCTTCAGCGTCATCCGACCATGCGTGTGTTCCATAAGTTAATGGATTACCACTTAAATGAAACGgaag                                                                                                                                                                                                                                                 <  1:12913/248‑1 (MQ=255) ggTCAGCGAAACGCTGGAAATGTTGCAAACCTGCTAGCAGCAGTTGGGTAAAACTGCCGAATGGGCAGAATTCCTGCAGCGTGCGGTGGAAGAGAACACCGGTGCCGATGCTGAATTGAGGCTTGCTGATATCATCGACGCGCGCGACGGTAGTGAGGCCGCACAGGTCTATATTACGCGCCAGCTTCAGCGTCATCCGACCATGCGTGGGTTCCATAAGTTAATGGATTACCACTTAATTGAAACGgaag                                                                                                                                                                                                                                                 <  2:481350/251‑1 (MQ=255)    cAGCGAAACGCTGGAAATGTTGCAAACCTGCTATCAGCAGTTGGGTAAAACTGCCGAATGGGCAGAATTCCTGCAGCGTGCGGTGGAAGAGAACACCGGTGCCGATGCTGAATTGCTGCTTGCTGATATCATCGAAGCGCGCGACGGTAGTGAGGCCGCACAGGTCTATATTACGCGCCAGCTTCAGCGTCATCCGACCATGCGTGTGTTCCATAAGTTAATGGATTACCACTTAAATGAAACGgaaga                                                                                                                                                                                                                                                >  2:309662/1‑249 (MQ=255)    cAGCGAAACGCTGGAAAGGTTGCAAACCTGCTATCAGCAGTTGGGTAAAACTGCCGAATGGGCAGAATTCTTGCAGCGTGCGGTGGAAGAGAACACCGGTGCCGATGCTGAATTGATGCTTGCTGATATCAGCGAAGCGCGCGACGGTAGTGAGGCCGCACAGGTCTATATTACGCGCCAGCTTCAGCGTCATCCGACCATGCTTGTGTGCCATAAGTTAATTGATTACCCCTTAAATGAAACGgaagaat                                                                                                                                                                                                                                              <  2:482670/251‑2 (MQ=255)                          cAAACCTGCTATCAGCAGTTGGGTAAAACTGCCGAATGGGCAGAATTCATGCAGCGTGCGGTGGAAGAGAACACCGGTGCCGATGCTGAATTGATGCTTGCTGATATCCTCGAAGCGCGCGACGGTAGTGAGGCCGCACAGGTCTATATTACGCGCCAGCTTCAGCGTCATCCGACCATGCGTGAGTTCCATAAGTTAATGGATTACCACTTAAATGAAACGGAAGAAGGGCGTGCCAAAGAGAGCCTGAt                                                                                                                                                                                                                        >  1:348637/1‑251 (MQ=255)                                 gCTATCAGCAGTTGGGTAAAACTGCCGAATGGGCAGAATTCCTGCAGCGTGCGGTGGAAGAGAACACCGGTGCCGATGCTGAATTGATGCTTGCTGATATCATCGAAGCGCGCGACGGTAGTGAGGCCGCACAGGTCTATATTACGCGCCAGCTTCAGCGTCATCCGACCATGCGTGTGTTCCATAAGTTAATGGATTACCACTTAAATGAAACGGAAGAAGGGCGTGCCAAAGAGAGCCTGATGGtgct                                                                                                                                                                                                                  <  2:90515/250‑1 (MQ=255)                                    aTCAGCAGTTGGGTAAAACTGCCGAATGGGCAGAATTCCTGCAGCGTGCGGTGGAAGAGAACACCGGTGCCGATGCTGAATTGATGCTTGCTGATATCATCGAAGCGCGCGACGGTAGTGAGGCCGCACAGGTCTATATTACGCGCCAGCTTCAGCGTCATCCGACCATGCGTGTGTTCCATAAGTTAATGGATTACCACTTAAATGAAACGGAAGAAGGGCGTGCCAAAGAGAGCCTGATGGTGCTGCGt                                                                                                                                                                                                              <  2:164391/251‑1 (MQ=255)                                          agTTGGGTAAAACTGCCGAATGGGCAGAATTCCTGCAGCGTGCGGTGGAAGAGAACACCGGTGCCGATGCTGAATTGATGCTTGCTGATATCATCGAAGCGCGCGACGGTAGTGAGGCCGCACAGGTCTATATTACGCGCCAGCTTCAGCGTCATCCGACCATGCGTGTGTTCCATAAGTTAATGGATTACCACTTAAATGAAACGGAAGAAGGGCGTGCCAAAGAGAGCCTGATGGTGCTGCGTGACATg                                                                                                                                                                                                        >  1:240578/1‑251 (MQ=255)                                           ggtGGGTAAAACTGCCGAATGGGCAGAATTCCTGCAGCGTGCGGTGGAAGAGAACACCGGTGCCGATGCTGAATTGATGCTTGCTGATATCATCGAAGCGCGCGACGGTAGTGAGGCCGCACAGGTCTATATTACGCGCCAGCTTCAGCGTCATCCGACCATGCGTGTGTTCCATAAGTTAATGGATTACCACTTAAATGAAACGGAAGAAGGGCGTGCCAAAGAGAGCCTGATGGTGCTGCGTGACATgg                                                                                                                                                                                                       <  2:172425/249‑1 (MQ=255)                                                       tGCCGAATGGGCAGAATTCCTGCAGCGTGCGGTGGAAGAGAACACCGGTGCCGATGCTGAATTGATGCTTGCTGATATCATCGAAGCGCGCGACGGTAGTGAGGCCGCACAGGTCTATATTACGCGCCAGCTTCAGCGTCATCCGACCATGCGTGTGTTCCATAAGTTAATGGATTACCACTTAAATGAAACGGAAGAAGGGCGTGCCAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAAgg                                                                                                                                                                                           >  1:126652/1‑251 (MQ=255)                                                               gggCAGAATTCCTGCAGCGTGCGGTGGAAGAGAACACCGGTGCCGATGCTGAATTGATGCTTGCTGATATCATCGAAGCGCGCGACGGTAGTGAGGCCGCACAGGTCTATATTACGCGCCAGCTTCAGCGTCATCCGACCATGCGTGTGTTCCATAAGTTAATGGATTACCACTTAAATGAAACGGAAGAAGGGCGTGCCAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAAGGTACgtagt                                                                                                                                                                                   >  2:390364/1‑251 (MQ=255)                                                                     aaTTCCTGCAGCGTGCGGTGGAAGAGAACACCGGTGCCGATGCTGAATTGATGCTTGCTGATATCATCGAAGCGCGCGACGGTAGTGAGGCCGCACAGGTCTATATTACGCGCCAGCTTCAGCGTCATCCGACCATGCGTGTGTTCCATAAGTTAATGGATTACCACTTAAATGAAACGGAAGAAGGGCGTGCCAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAAGGTACGTAGTAAGCCt                                                                                                                                                                             <  1:132613/251‑1 (MQ=255)                                                                         ccTGCAGCGTGCGGTGGAAGAGAACACCGGTGCCGATGCTGAATTGATGCTTGCTGATATCATCGAAGCGCGCGACGGTAGTGAGGCCGCACAGGTCTATATTACGCGCCAGCTTCAGCGTCATCCGACCATGCGTGTGTTCCATAAGTTAATGGATTACCACTTAAATGAAACGGAAGAAGGGCGTGCCAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAAGGTACGTAGTAAGCCTCGtt                                                                                                                                                                         <  1:102567/251‑1 (MQ=255)                                                                         ccTGCAGCGTGCGGTGGAAGAGAACACCGGTGCCGATGCTGAATTGATGCTTGCTGATATCATCGAAGCGCGCGACGGTAGTGAGGCCGCACAGGTCTATATTACGCGCCAGCTTCAGCGTCATCCGACCATGCGTGTGTTCCATAAGTTAATGGATTACCACTTAAATGAAACGGAAGAAGGGCGTGCCAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAAGGTACGTAGTAAGCCTCGt                                                                                                                                                                          <  2:125967/250‑1 (MQ=255)                                                                            gCAGCGTGCGGTGGAAGAGAACACCGGTGCCGATGCTGAATTGATGCTTGCTGATATCATCGAAGCGCGCGACGGTAGTGAGGCCGCACAGGTCTATATTACGCGCCAGCTTCAGCGTCATCCGACCATGCGTGTGTTCCATAAGTTAATGGATTACCACTTAAATGAAACGGAAGAAGGGCGTGCCAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAAGGTACGTAGTAAGCCTCGTTAt                                                                                                                                                                       <  2:288184/250‑1 (MQ=255)                                                                                     ggTGGAAGAGAACACCGGGGCCGATGCTGAATTGATGCTTGCTGATATCATCGAAGCGCGCGACGGTAGTGAGGCCGCACAGGTCTATATTACGCGCCAGCTTCAGCGTCATCCGACCATGCGTGTGTTCCATAAGTTAATGGATTACCACTTAAATGAAACGGAAGAAGGGCGTGCCAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAAGGTACGTAGTAAGCCTCGTTATCGTTGcc                                                                                                                                                                >  1:414714/1‑248 (MQ=255)                                                                                       gggAAGAGAACACCGGTGCCGATGCTGAATTGATGCTTGCTGATATCATCGAAGCGCGCGACGGTAGTGAGGCCGCACAGGTCTATATTACGCGCCAGCTTCAGCGTCATCCGACCATGCGTGTGTTCCATAAGTTAATGGATTACCACTTAAATGAAACGGAAGAAGGGCGTGCCAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAAGGTACGTAGTAAGCCTCGTTATCGTTGCCAGaaa                                                                                                                                                           <  2:339932/250‑1 (MQ=255)                                                                                             agaACACCGGTGCCGATGCTGAATTGATGCTTGCTGATATCATCGAAGCGCGCGACGGTAGTGAGGCCGCACAGGTCTATATTACGCGCCAGCTTCAGCGTCATCCGACCATGCGTGTGTTCCATAAGTTAATGGATTACCACTTAAATGAAACGGAAGAAGGGCGTGCCAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAAGGTa                                                                                                                                                                                         >  1:10018/1‑215 (MQ=255)                                                                                             agaACACCGGTGCCGATGCTGAATTGATGCTTGCTGATATCATCGAAGCGCGCGACGGTAGTGAGGCCGCACAGGTCTATATTACGCGCCAGCTTCAGCGTCATCCGACCATGCGTGTGTTCCATAAGTTAATGGATTACCACTTAAATGAAACGGAAGAAGGGCGTGCCAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAAGGTa                                                                                                                                                                                         <  2:10018/215‑1 (MQ=255)                                                                                                   ccGGTGCCGATGCTGAAGTGATGCTTGCTGATATCATCGAAGCGCGCGACGGTAGTGAGGCCGCACAGGTCTATATTACGCGCCAGCTTCAGCGTCATCCGACCATGCGTGTGTTCCATAAGTTAATGGATTACCACTTAAATGAAACGGAAGAAGGGCGTGCCAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAAGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTAcc                                                                                                                                               <  1:458184/251‑1 (MQ=255)                                                                                                        gCCGATGCTGAATTGATGCTTGCTGATATCATCGAAGCGCGCGACGGTAGTGAGGCCGCACAGGTCTATATTACGCGCCAGCTTCAGCGTCATCCGACCATGCGTGTGTTCCATAAGTTAATGGATTACCACTTAAATGAAACGGAAGAAGGGCGTGCCAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAAGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCAt                                                                                                                                           >  2:65237/1‑250 (MQ=255)                                                                                                          cGATGCTGAATTGATGCTTGCTGATATCATCGAAGCGCGCGACGGTAGTGAGGCCGCACAGGTCTATATTACGCGCCAGCTTCAGCGTCATCCGACCATGCGTGTGTTCCATAAGTTAATGGATTACCACTTAAATGAAACGGAAGAAGGGCGTGCCAAAGAGAGCCTGATGGTGCTGAGTGACATGGTTGGCGAGAAGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCATacc                                                                                                                                        >  2:77073/1‑250 (MQ=255)                                                                                                                tGAATTGATGCTTGCTGATATCATCGAAGCGCGCGACGGTAGTGAGGCCGCACAGGTCTATATTACGCGCCAGCTTCAGCGTCATCCGACCATGCGTGTGTTCCATAAGTTAATGGATTACCACTTAAATGAAACGGAAGAAGGGCGTGCCAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAAGGAACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCATACACCctct                                                                                                                                  >  2:127230/1‑251 (MQ=255)                                                                                                                     tGATGCTTGCTGATATCATCGAAGCGCGCGACGGTAGTGAGGCCGCACAGGTCTATATTACGCGCCAGCTTCAGCGTCATCCGACCATGCGTGTGTTCCATAAGTTAATGGATTACCACTTAAATGAAACGGAAGAAGGGCGTGCCAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAAGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGTTTTTACCGCATACACCCTCTACTg                                                                                                                              <  1:390364/250‑1 (MQ=255)                                                                                                                                     catcGAAGCGCTCGACGGTAGTGAGGCCGCACGGGTCTATATTACGCGCCAGCTTCAGCGGCATCCGACCATGCGTGTGTTCCATAAGTTAATGGATTACCACTTAAATGAAACGGAATAAGGGCGTGCCAAAGAGAGCCTGAGGGTGCTGCGTGACATGGTTGGCGAGAAGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCATACACCCTCTACTGGCATTGTCCGTCTTGTc                                                                                                             <  1:41888/251‑1 (MQ=255)                                                                                                                                        cGAAGCGCGCGACGGGGGTGAGGCCGCACAGGTCTATATTACGCGCCAGCTTCAGCGTCATCCGACCATGCGTGTGTTCAATAAGTTAATGGATTCCCACTTAAATGAAACGGAAGAAGGGCGTGCCAAAGAGAGCCTGCTGGTGATGCGTGACATGGTTGGCGAGAAGGTACGTAGTAAGCCTCGTTATCGTTGCCAGCAATGTGGTTTAACCGCATACACCCTCTACTGGCATTGTCCGTCTTGTCggg                                                                                                          >  1:68265/1‑251 (MQ=255)                                                                                                                                           aGCGCGCGACGGTAGTGAGGCCGCACAGGTCTATATTACGCGCCAGCTTCAGCGTCATCCGACCATGCGGGTGTTCCATAAGTTAATGGATTACCACTTAAATGAAACGGAAGACGGGCGTGCAACAGAGAACCTGATGGTGCTGCGTGACAAGGTTGTCGAGAAGATACGGAGTCAGCATCGTTATCGTTGCCAGAAATGTGGTTTTACCGCATACAACCTCTACTGGCTTTGTCAGTCTTGTCGGGCCt                                                                                                       >  2:174867/1‑251 (MQ=255)                                                                                                                                                         gTGAGGGCGCACAGTTCGATATTACGCGGCAGCTTCAGCGTCATCCGACCATGCGTGTGTTCCATAAGTTAATGGATTACCACTTAAATGAAACGGAAGAAGGGCGTGCCAAAGAGAGCCTGATGGTGGTGCGTGACATGGTTGGCGATAAGGTACGTAGTAAGCCCCGTTATCGTTGCCAGACATGTGGTTTGACCGCATACACCCTCTACTGGCATTGTCCGTCTTGTCGGGCCTGGTCAATCATTaaa                                                                                         <  2:180433/251‑1 (MQ=255)                                                                                                                                                               ccGCAAAGGTCTATATTCCGCGCCAGCTTCAGCGTCATCCGACCATGCGTGTGTTCCATAAGTTAATGGATTACCACTTAAATGAAACGGAAGAAGGGCGTGCCAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAAGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCATACACCCTCTACTGGCATTGTCCGTATTGTCGGGCCTGGTCAACCATTAAACCGAtt                                                                                   <  2:369851/251‑1 (MQ=255)                                                                                                                                                                   acaGGTCTATATTACGCGCCAGCTTCAGCGTCATCCGACCATGCGTGTGTTCCATAAGTTAATGGATTACCACTTAAATGAAGCGGAAGAAGGGCGTGCCAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAAGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCATACACCCTCTACTGGCATTGTCCGTCTTGTCGGGCCTGGTCAACCATTAAACCGATTcgcg                                                                               <  1:172646/251‑1 (MQ=255)                                                                                                                                                                       gttaatatTTCGCGCCAGCTTCAGCGTCATCCGAACATGCGTGTGTTCCCTAAGTTAATTGATTACCACATAAATGAAACGGAAGAAGGGCGTGCCAAAGAGAGCCTGATGTTGCTGCGTGACATGGTTGGCGAGAATGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCAGACCCCCTCGACTGGCATTGTCCGTCTTGTCGGGCCTGGTCAACCATTAAACCGATTCGCggcct                                                                           <  2:348940/247‑4 (MQ=255)                                                                                                                                                                                                       gaacATGCGTGTGGTCCAGTAGTTAATTGATTACCACTTAAATGAAACGGAAGAAGGGCGTGCCAAAGAGAGGCTGATGGTGCTGCGTTACATGGTTTGCGAGAAGGTGCGTAGTAAGCCCCGTTATTGTTGTCAGTAATGTTGTTTTTCCGCATACTCCGTCTACTGGCCTTGTCCGTCTTGTCGGGCCTGGTCAACCATTAACCCGATTCGCGGTCTTGATGGCCTGTAATTTTTAAAAAAAATCCgtc                                           <  2:251712/248‑3 (MQ=255)                                                                                                                                                                                                       gACCATGCGTGTGTTCCATAAGTTAATGGATTACCACTTAAATGAAACGGAAGAAGGGCGTGCCAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAAGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCATACACCCTCTACTGGCATTGTCCGTCTTGTCGGGCCTGGTCAACCATTAAACCGATTCGCGGTCTTGATGGCCTGTAATTTTTAAAAAAAATCCGAc                                           <  1:149537/251‑1 (MQ=255)                                                                                                                                                                                                        aCCATGCGTGTGTTCCATAAGTTAATGGATTACCACTTAAATGAAACGGAAGAAGGGCGTGCCAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAAGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCATACACCCTCTACTGGCATTGTCCGTCTTGTCGGGCCTGGTCAACCATTAAACCGATTCGCGGTCTTGATGGCCTGTAATTTTTAAAAAAAATCCGACt                                          <  1:65237/251‑1 (MQ=255)                                                                                                                                                                                                              cGTGTGTTCCATAATGTAATGGATTACCACCTAAATGAAACGGAAGAAGGGCGTGCCAAATAGAGCCTGATGGTGCTGCGTTACATGGTTGGCGAGAAGGTACGTAGTAAGCCTCGTTATCTTTTCCCGAAATGTGGTTTTACCGCATACACCCTCTACTGGCATTGTCCGTCTTGTCGGGCCTGGTCAACCATTAAACCGATTCGCGGTCTTGATGGCCTGTAATTTTTAAAAAAAATCCGACTTTAGtt                                    <  2:52107/251‑1 (MQ=255)                                                                                                                                                                                                                    ttCCATAAGTTAATGGATTACCACTTAAATGACACGGAAGAAGGGCGTGCCAAAGAGAGCCTGATGGTGCAGCGTGACATGGTTGGCGAGAAGGAACGTAGTAAGCCTCGTTATCGTTGACAGAAATGTGGTTTTACCGCATACACCCTCTACTGGCATTGTCCGTCTTGTCGGGCCTGGTCAACCATTAAACCGATTCGCGGGCTTGATGGCCTGTAATTTTTACAAAAAATCCGACTTTAGTTacaac                               >  1:290530/1‑250 (MQ=255)                                                                                                                                                                                                                           aGTTAATGGATTACCACTTAAATGAAACGGAAGAAGGGCGTGCCAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAAGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCATACACCCTCTACTGGCATTGTCCGTCTTGTCGGGCCTGGTCAACCATTAAACCGATTCGCGGTCTTGATGGCCTGTAATTTTTAAAAAAAATCCGACTTTAGTTACAACATACTAat                       <  1:309662/251‑1 (MQ=255)                                                                                                                                                                                                                                aTGGATTACCACTTAAATGAAGCGGAAGAAGGGCGTGCCAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAAGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCATACACCCTCTACTGGCATTGTCCGTCTTGTCgg                                                                                                           <  2:91788/162‑1 (MQ=255)                                                                                                                                                                                                                                aTGGATTACCACTTAAATGAAGCGGAAGAAGGGCGTGCCAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAAGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCATACACCCTCTACTGGCATTGTCCGTCTTGTCgg                                                                                                           >  1:91788/1‑162 (MQ=255)                                                                                                                                                                                                                                aTGGATTACCAATTAAATGAAACGGAAGAAGGGCGTGCCAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAAGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCTCATACACCCGCTACTGGCATTGTCCGTCTTGTCGGGCCTGGTCAACCATTAAACCGATTCGCGGTCTTGATGTCCTTTAATTTTTAAAAAAAATCCTGCTTTAGTTACAACATACTAAttatta                  <  1:425928/251‑1 (MQ=255)                                                                                                                                                                                                                                    aTTACCAATTAAATGAAACGGAAGAAGGGCGTGCCAAAGAGAGCCTGATGGTACTGCGTGACATGGTTGGCGAGAAGGTACGGAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCATACACCCTCTACTGGCATTGTCCGTCTTGTCGGGCCTGGTCAACCATTAAACCGATTCGCGGTCTTGATGGCCTGTAATTTTTAAAAAAAATCAGACTATAGCACCAACATATTCATTATTAATGt              >  2:335739/1‑251 (MQ=255)                                                                                                                                                                                                                                           cTTAAATGAAACGGAAGAAGGGCGTGCCAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAAGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCATACACCCTCTACTGGCATTGTCCGTCTTGTCGGGCCTGGTCAACCATTAAACCGATTCGCGGTCTTGATGGCCTGTAATTTTTAAAAAAAATCCGACTTTAGTTACAACATACTAATTATTAATGTTCCATtg       >  1:194920/1‑251 (MQ=255)                                                                                                                                                                                                                                             tAAATGAAACGGAAGAAGGGCGTGCCAAAGAGAGCCTGATTGTGCTGCGTGACATGGTTTGTGAGAAGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTGTTACCGCATACGCTCTCTACTTGCATTGTCCGGCTTGGCGGGCCTGGTCAACCATTAAACTGATTTGTGTTCTTGATGGCGTGGAATTTCTAAAAAAAATCCGACTTTAGTTACAACATACTAATTATTAATGTTCCATtg       <  1:472876/249‑1 (MQ=255)                                                                                                                                                                                                                                               aaTGAAACGGAAGAAGGGCGTGCCAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAAGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCATACACCCTCTACTGGCATTGTCCGTCTTGTCGGGCCTGGTCAACCATTAAACCGATTCGCGGTCTTGATGGCCTGTAATTTTTAAAAAAAATCCGACTTTAGTTACAACATACTAATTATTAATGTTCCa          >  1:217175/1‑244 (MQ=255)                                                                                                                                                                                                                                               aaTGAAACGGAAGAAGGGCGTGCCAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAAGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCATACACCCTCTACTGGCATTGTCCGTCTTGTCGGGCCTGGTCAACCATTAAACCGATTCGCGGTCTTGATGGCCTGTAATTTTTAAAAAAAATCCGACTTTAGTTACAACATACTAATTATTAATGTTCCATTGTGCt   >  1:88343/1‑251 (MQ=255)                                                                                                                                                                                                                                               aaTGAAACGGAAGAAGGGCGTGCCAAAGAGAGCCTGATGGTGCTGCGGGACATGGTTGGCGAGAAGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCATACACCCTCTACTGGCATTGTCCGTCTTGTCGGGCCTGGTCAACCATTAAACCGATTCGCGGTCTGGATGGCCTGTAATTTTTAAAAAAAATCCGACTTTAGTTACAACATACTAATTATTAATGTTCCa          <  2:217175/244‑1 (MQ=255)                                                                                                                                                                                                                                                aTGAAACGGAAGAAGGGCGTGCCAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAAGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCATACACCCTCTACTGGCATTGTCCGTCTTGTCGGGCCTGGTCAACCATTAAACCGATTCGCGGTCTTGATGGCCTGTAATTTTTAAAAAAAATCCGACTTTAGTTACAACATACTAATTATTAATGTTCCATTGTGCTc  >  1:5652/1‑251 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                      GGTCAGCGAAACGCTGGAAATGTTGCAAACCTGCTATCAGCAGTTGGGTAAAACTGCCGAATGGGCAGAATTCCTGCAGCGTGCGGTGGAAGAGAACACCGGTGCCGATGCTGAATTGATGCTTGCTGATATCATCGAAGCGCGCGACGGTAGTGAGGCCGCACAGGTCTATATTACGCGCCAGCTTCAGCGTCATCCGACCATGCGTGTGTTCCATAAGTTAATGGATTACCACTTAAATGAAGCGGAAGAAGGGCGTGCCAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAAGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCATACACCCTCTACTGGCATTGTCCGTCTTGTCGGGCCTGGTCAACCATTAAACCGATTCGCGGTCTTGATGGCCTGTAATTTTTAAAAAAAATCCGACTTTAGTTACAACATACTAATTATTAATGTTCCATTGTGCTC  >  NC_000913/1341298‑1341787

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑