Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,323,798	G→A	K193K (AAG→AAA)	rnm →	RNase AM

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,323,798	0	G	A	92.3%	103.1 / ‑1.1	39	K193K (AAG→AAA)	rnm	RNase AM
Reads supporting (aligned to +/- strand):  ref base G (1/2);  new base A (18/18);  total (19/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.62e-01

AAAGACTTGAAAAAGCGCAAATACCTGGGGCGCTGGAAGGCGCACAACGACTGGCGCAGGGCGGCGCAGTGACGCGCGGTCATTTTGCGCGTTTCCTTGTTGAGTGCGGCAAAGCCAGTTCAATGGCGGATGTCTTTAAAAAGTATCTGGCGCGCGGGAAAACCGGATACGTTCCGCCACAGTGGTGTACAATAGAACAAGCTATTGATGTCATTCATCATTCTGGCGGTAAGGCGGTGCTAGCTCATCCTGGGCGGTACAATCTTAGTGCTAAATGGCTGAAAAGATTGGTTGCGCATTTTGCCGAACACCACGGTGACGCGATGGAAGTCGCGCAGTGCCAGCAATCGCCCAATGAACGTACCCAGCTGGCGGCCCTTGCGCGTCAGCATCATTTATGGGCATCACAAGGATCTGATTTTCATCAGCCATGCCCGTGGATCGAACTGGGTCGTAAACTCTGGCTGC  >  NC_000913/1323566‑1324033                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                            aaaGACTTGAAAAAGCGCAAATACCTGGGGCGCTGGAAGGCGCACAACGACTGGCGCAGGGCGGCGCAGTGACGCGCGGTCATTTTGCGCGTTTCCTTGTTGAGTGCGGCAAAGCCAGTTCAATGGCGGATGTCTTTAAAAAGTATCTGGCGCGCGGGAAAACCGGATACGTTCCGCCACAGTGGTGTACAATAGAACAAGCTATTGATGTCATTCATCATTCTGGCGGTAAAGCGGTGCTAGCTCATCCt                                                                                                                                                                                                                           <  1:226935/251‑1 (MQ=255)   aGACTTGAAAAAGCGCAAATACCTGGGGCGCTGGAAGGCGCACAACGACTGGCGCAGGGCGGCGCAGTGACGCGCGGTCATTTTGCGCGTTTCCTTGTTGAGTGCGGCAAAGCCAGTTCAATGGCGGATGTCTTTAAAAAGTATCTTGCGCGGGGGAAAAACGGGTAAGTTCCGCCACAGTGGTGTACAAAAGAACAAGCTATTGATTTCATTCATCATTCTGGCGGTAAAGCGGTGATAGGTCAtacttg                                                                                                                                                                                                                         >  1:140656/1‑246 (MQ=255)   aGACTTGAAAAAGCGCAAATACCTGGGGCGCTGGAAGGCGCACAACGACTGGCGCAGGGCGGCGCAGTGACGCGCGGTCATTTTGCGCGTTTCCTTGTTGAGTGCGGCAAAGCCAGTTCAATGGCGGATGTCTTTAAAAAGTATCTGGCGCGCGGGAAAACCG‑ATAAGTTTCGGCACAGTGGCGGTCAAATGAAAAAGCCTTTGATGTTTTTCATTCTTCTTGCGGTAAGGCggatgcagcccaatcctgg                                                                                                                                                                                                                        >  1:214550/1‑234 (MQ=255)      cTTGAAAAAGCGCAAATACCTGGGGAGCTGGAAGGCGCACAACGACTGGCGCAGGGCGGCGCAGTGACGCGCGGTCATTTTGCGCGTTTCCTTGTTGAGGGCGGCAAAGCCAGTTCAATGGCGGCTGGCTTTAAAAAGTATCTGGCGCGCGGGCAAACCGGGTCCGTTCCGCCACAGTTGTGTACAATAGAACAAGCTCTTGATGTACTTCATCCTTCTGGCGGGAAAGCGGGGCTGGCGCATCCTGGGCg                                                                                                                                                                                                                      >  2:196306/1‑251 (MQ=255)         gAAAAAGCGCAAATACCTGGGGCGCTGGAAGGCGCACAACGACTGGCGCAGGGCGGCGCAGTGACGCGCGGTCATTTTGCGCGTTTCCTTGTTGAGTGCGGCAAAGCCAGTTCAATGGCGGATGTCTTTAAAAAGTATCTGGCGCGCGGGAAAACCGGATACGTTCCGCCACAGGGGGGTCAAATAGAACAAGCTATTGATGTCATTCATAATTTTGGCGGGCAAGCGGTGCTAACTCATCCCGGGCGGt                                                                                                                                                                                                                    >  2:454165/1‑250 (MQ=255)         gAAAAAGCGCAAATACCTGGGGCGCTGGAAGGCGCACAACGACTGGCGCAGGGCGGCGCAGTGACGCGCGGTCATTTTGCGCGTTTCCTTGTTGAGTGCGGCAAAGCCAGTTCAATGGCGGATGTCTTTAAAAAGAATCTGGCGCGCGGGAAAACCGGATACGTTCCGCCACAGGGGTGTACAATAGAAAAAGCTATTGCTGTCATTCATCATTCTGGCGGAATAACGGGGCTAGCTCTTCCTGGGCgga                                                                                                                                                                                                                    >  2:327904/1‑249 (MQ=255)            aaaGCGCAAAGTCCTGGGGCGCTGGAAGGCGCACAACGACTGGCGCAGGGCGGCGCAGTGACGCGCGGTCATTTTGCGCGTTTCCTTGTTGAGTGCGGCAAAGCCAGTTCAATGGCGGATGTCTTTAAAAAGTATCTGGCGCGCGGGAAAACCGGATACGTTCCGCCACAGTGGTGTACAATAGAACAAGCTATTGATGTCATTCATCATTCTGGCGGTAAAGCGGTGCTAGCTCATCCTGGGCGGTACaa                                                                                                                                                                                                                <  2:57616/251‑1 (MQ=255)                   ccgagcttGGGGCGCTGGAAGGCGCACTACGACTGGCGCAGGGCGGCGCAGTGACGCGCGGTCATTTTGCGCGTTTCCTTGTTGAGTGCGGCAAAGCCAGTTCAATGGCGGATGTCTTTAAAAAGTATCTGGCGCGCGGGAAAACCGGATACGTTCCGCCACAGTGGTGTACAATAGAACAAGCTATTGATGTCATTCATCATTCTGGCGGTAAAGCGGTGCTAGCTCATCCTGGGCGGTACAATCTTAGt                                                                                                                                                                                                         <  2:331191/244‑1 (MQ=255)                   aaaTACCTGGGGCGCTGGAAGGCGCACAACGACTGGCGCAGGGCGGCGCAGTGACGCGCGGTCATTTTGCGCGTTTCCTTGTTGAGTGCGGCAAAGCCAGTTCAATGGCGGATGTCTTTAAAAAGTATCTGGCGCGCGGGAAAACCGGATACGTTCCGCCACAGTGGTGTACAATAGAACAAGCTATTGATGTCATTCATCATTCTGGCGGTAAAGCGGTGCTAGCTCATCCTGGGCGGTACAATCTTAGt                                                                                                                                                                                                         <  1:207014/251‑1 (MQ=255)                          tGGGGCGCTGGAAGGCGCACAACGACTGGCGCAGGGCGGCGCAGTGACGCGCGGTCATTTTGCGCGTTTCCTTGTTGAGTGCGGCAAAGCCAGTTCAATGGCGGATGTCTTTAAAAAGTATCTGGCGCGCGGGAAAACCGGATACGTTCCGCCACAGTGGTGTACAATAGAACAAGCTATTGATGTCATTCATCATTCTGGCGGTAAAGCGGTGCTAGCTCCTCCTGGGCGGTACAATCttcgt                                                                                                                                                                                                         >  1:331191/1‑241 (MQ=255)                            gggCGCTGGAAGGCGCACAACGACTGGCGCAGGGCGGCGCAGTGACGCGCGGTCATTTTGCGCGTTTCCTTGTTGAGTGCGGCAAAGCCAGTTCAATGGCGGATGTCTTTAAAAAGTATCTGGCGCGCGGGAAAACCGGATACGTTCCGCCACAGTGGTGTACAATAGAACAAGCTATTGATGTCATTCATCATTCTGGCGGTAAAGCGGTGCTAGCTCATCCTGGGCGGTACAATCTTAGTGCTAAATgg                                                                                                                                                                                                <  1:475771/251‑1 (MQ=255)                               cgcTGGAAGGCGCACAACGACTGGCGCAGGGCGGCGCAGTGACGCGCGGTCATTTTGCGCGTTTCCTTGTTGAGTGCGGCAAAGCCAGTTCAATGGCGGATGTCTTTAAAAAGTATCTGGCGCGCGGGAAAACCGGATACGTTCCGCCACAGTGGTGTACAATAGAACAAGCTATTGATGTCATTCATCATTCTGGCGGTAAAGCGGTGCTAGCTCATCCTGTGCGGTACAATCTTAGTGCTAAATGGCTg                                                                                                                                                                                             <  1:118622/251‑1 (MQ=255)                                gcTGGAAGGCGCACAACGACGGGCGCAGGGCGGCGCAGTGACGCGCGGTCATTTTGGGCGTTTCCTTGTTGAGTGCGGCAAAGCCAGTTCAATGGCGGATGTCTTTAAAAAGGATCTGGCGCGCGGGAAAACCGGATACGTTCCGCCACAGTGGTGTACAATAGAACAAGCTATTGATGTCATTCATCATTCTGGCGGTAAAGCGGTGCTAGCTCATCCTGGGCGGTACAATCTTAGTGCTAAATGGCTGa                                                                                                                                                                                            <  2:104930/251‑1 (MQ=255)                                         cgcACAACGACTGGCGCAGGGCGGCGCAGTGACGCGCGGTCATTTTGCGCGTTTCCTTGTTGAGTGCGGCAAAGCCAGTTCAATGGCGGATGTCTTTAAAAAGTATCTGGCGCGCGGGAAAACCGGATACGTTCCGCCACAGTGGTGTACAATAGAACAAGCTATTGATGTCATTCATCATTCTGGCGGTAAAGCGGTGCTAGATCATCCTGGGCGGTAAAATCTTAGTGCTAAATGGCTGAAAAGATTgg                                                                                                                                                                                   >  1:20786/1‑251 (MQ=255)                                           cacaACGACTGGCGCAGGGCGGCGCAGTGACGCGCGGTCATTTTGCGCGTTTCCTTGTTGAGTGCGGCAAAGCCAGTTCAATGGCGGATGTCTTTAAAAAGTATCTGGCGCGCGGGAAAACCGGATACGTTCCGCCACAGTGGTGTACAATAGAACAAGCTATTGATGTCATTCATCATTCTGGCGGTAAGGCGGTACTAGCTCATCCTGGGCGGTACAATCTTAGTGCTAAATGGCTGAAAAGATTGGtt                                                                                                                                                                                 <  2:240592/251‑1 (MQ=255)                                                        gcAGGGCGGGGCAGTGACGCGCGGTCATTTTGGGCGGTTCCTGGTTGAGTGCGGCAAGGCCAGTTCAATGGCGGATGTCTTTAAAAAGTATCTGGCGCGCGGGAAAACCGGATACGTTCCGCCACAGTGGTGTACAATAGAACAAGCTATTGATGTCATTCATCATTCTGGCGGTAAAGCGGTGCTAGCTCATCCTGGGCGGTACAATCTTAGTGCTAAATGGCTGAAAAGATTGGTTGCGCATTTTGCCg                                                                                                                                                                    <  2:129977/251‑1 (MQ=255)                                                        gcAGGGCGGCGCAGTGACCTGCGGTCATTTTGCGCGTTTCCTTGTTTAGTGCGGCAAAGCCAGTTCAATGGCGGATGTCTTTAAAAAGTATCTGGCGCGCGGGAAAACCGGATACGTTCCGCCACAGTGGTGTACAATAGAACAAGCTATTGATGTCATTCATCATTCTGGCGGTAAAGCGGTGCTAGCTCATCCTGGGCGTTACAATCTTAGTGCTAAATGGCTGAAAATATTTGTTTCGCATTTTGcct                                                                                                                                                                    <  2:215739/251‑2 (MQ=255)                                                         cAGGGCGGCGCAGTGACGCGCGGTCATTTTGCGCGTTTCCTTGTTGAGTGCGGCAAAGCCAGTTCAATGGCGGATGTCTTTAAAAAGTATCTGGCGCGCGGGAAAACCGGATACGTTCCGCCACAGTGGTGTACAATAGAACAAGCTATTGATGTCCTTCATCATTCTGGCGGTAAAGCGGTGCTAGCTCATCCTGGGCGGTACCATCTTAGTGCTAAATGGCTGAAAAGATTGGTTGCGCATTTTTCCg                                                                                                                                                                    >  1:278525/1‑250 (MQ=255)                                                                      tGACGCGCGGTTATTTTGCGCGTTTCCTTGTTGAGTGCTGCAAAGCCAGTTCAATGGCGGATGGCTTTAAAAAGTATCTGGCGCGCGGGAAAACCGGATACGTTCCGCCACAGTGGTGTACAATAGAACAAGCTATTGATGTCATTCATCATTCTGGCGGTAAAGCGGTGCTAGCTCATCCTGGGCGGTACAATCTTAGTGCTAAATGGCTGAAAAGATTGGTGGCGCATTTTGCCGAACACCACGGTGa                                                                                                                                                       <  1:66478/250‑1 (MQ=255)                                                                            gcgGTCATTTTGCGCGTTTCCTTGTTGAGTGCGGCAAAGCCAGTTCAATGGCGGATGTCTTTAAAAAGTATCTGGCGCGCGGGAAAACCGGATACGTTCCGCCACAGTGGGGTACAATAGAACAAGCTATTGATGTCATTCATCATTCTGGCGGTAAAGCGGGGCTAGCTCATCCTGGGCGGTACAATCTTAGTGCTAAATGGCTGAAAAGCTTGGTTGCGCATTTTGCCGAACACCCCGGGGACGCGATg                                                                                                                                                >  2:179124/1‑251 (MQ=255)                                                                              ggTCATTTTGCGCGTTTCCTTGTTGAGTGCGGCAAAGCCAGTTCAATGGCGGATGTCTTTAAAAAGTATCTGGCGCGCGGGGAAACCGGATACGTTCCGCCACAGTGGTGTACAATAGAACAAGCTATTGATGTCATTCATCATTCTGGCGGTAAAGCGGGGCTAGCTCATCCTGGGCGGTACAATCTTAGTGCTAAATGGCTGAAAAGATTGGTTGCGCATTTTTCCGAACACCCCGGTGACGCGATgg                                                                                                                                               >  2:472538/1‑250 (MQ=255)                                                                                       gcgcGGTTCCTTGTTGAGTGCGGCAAAGCCAGTTCAATGGCGGATGTCTTTAAAAAGTATCTGGCGCGCGGGAAAACCGGATACGTTCCGCCACAGTGGTGTACAATAGAACAAGCTATTGATGTCATTCATCATTCTGGCGGTAAAGCGGTGCTAGCTCATCCTGGGCGGTACAATCTTAGTGCTAAATGGCTGAAAAGATTGGTTGCGCATTTTGCCGAACACCACGGTGACGCGATGGAAGTCGCGCa                                                                                                                                     <  1:472538/251‑1 (MQ=255)                                                                                                 ttgttgAGTGCGGCAAAGCCAGTTCAATGGCGGCTGTCTTTAAAAAGAATCTGGCGCGCGGGAAAACCGGATACGTTCCGCCAAAGGGGTGTACAATAGAACAAGCTATTGATGTCATTCATCATTCTGGCGGTAAAGAGGTGCTAGCTCATCCTGGGCGGTACAATCTTAGTGCTCAATGGCTGAAAAGATTGGGTGAGCTTTTTGCCGAACACCACGGTGACGCGAGGGAAGTTGCGCAGTGCCAGCa                                                                                                                            >  1:259020/1‑250 (MQ=255)                                                                                                             gCAAAGCCAGTTCAATGGCGGATGGCTTTAAAAAGTAGCTGGCGCGCGGGAAAACCGGATACGTTCCGCCACAGTGGTGTACAATAGAACAAGCTATTGATGTCATTCATCATTCTTGCGGTAAAGCGGTGCTAGCTCATCCTGGGCGGTACAATCTTAGTGCTAAATGGCTGAAAAGATTGGTTGCGCATTTTGCCGAACACCACGGTGACGCGATGGAAGTCGCGCAGTGCCAGCAATCGCCCAATGaa                                                                                                               <  1:69432/251‑1 (MQ=255)                                                                                                              cAAAGCCAAGTCAATGGCGGATGTCGTTAAAAAGTATCTGGCGCGCGGGAAAACCGGATACGGTCCGCCACAGTGGGGTACAAGAGAACAAGCTATTGATGTCATTCATCATTCTGGCGGTAAAGCGGTGCTAGCTCATCCTGGGCGGGACAATCTTAGTGCTAAATGGCTGAAAAGATAGGTTGCGCATTTTGCCGAACACCACGGTGACGCGATTGAAGTCGCGCGGGGCCTGCAATCGCCTAATGAAc                                                                                                              <  2:307561/251‑1 (MQ=255)                                                                                                                                 gATGTCTTTAAAAAGTATCTGGCGCGCGGGAAAACCGGATACGTTCCGCCACAGTGGTGTACAATAGAACAAGCTATTGATGTCATTCATCATTCTGGCGGTAAAGCGGTGCTAGCTCATCCTGGGCGGTACAATCTTAGTGCTAAATGGCTGAAAAGATTGGTTGCGCATTTTGCCGAACACCACGGTGACGCGATGGAAGTCGCGCAGTGCCAGCAATCGCACCATGAACGTACCCAGCTGGCGGCCCt                                                                                           >  2:326267/1‑251 (MQ=255)                                                                                                                                 gATGTATTTAAAAAGTATCTGGCGCGCGGGAAACCCGGATACGTTCCGCCACAGTGGTGTACACGAGAACAAGCTATTGATGTACTTCATCATTCTGGCGGTAAAGCGGTGCTAGCTCATCCTGGGCTGTACAATCTTAGTGCTAAATGGCTGAAAAGATTGGTTGCGCATTTTGCCGAACACCACGGTGCCGCGATGGAAGACTCGCAGGGCCAGCACTCCCCCAATGAACGTAACCCGCTGGCGCCCCt                                                                                           >  2:23705/1‑251 (MQ=255)                                                                                                                                                   cTGGCGCGCGGGAAAACCGGATACGTTCCGCCACAGTGGTGTACAATAGAACAAGCTATTGATGTCATTCATCATTCTGGCGGTAAAGCGGTGCTAGCTCATCCTGGGCGGTACAATCTTAGTGCTAAATGGCTGAAAAGATTGGTTGCGCATTTTGCCGAACACCACGGTGACGCGATGGAAGTCGCGCAGTGCCAGCAATCGCCCAATGAACGTACCCAGCTGGCGGCCCTTGCGCGTCAGCATCAttt                                                                         >  1:330848/1‑251 (MQ=255)                                                                                                                                                      ggggcacgggaaaCCGGATACGTTCCGCCACAGTGGTGTACAATAGAACAAGCTATTGATGTTATTCATCATTCTGGCGGGAAGGCGGTGCTAGCTCATCCTGGGCGGTACAATCTTAGTGCTAAATGGCTGAAAAGATTGGTTGCGCATTTTGCCGAACACCACGGTGACGCGATGGAAGTCGCGCAGTGCCAGCAATCGCCCAATGAACGTACCCAGCTGGCGGCCCTTGCGCGTCAGCATCATTTagg                                                                      <  2:214550/241‑3 (MQ=255)                                                                                                                                                             ggAAAACCGGATACTGTCCGCCTCAGTGTTGTACAATAGAACACGCTATTGGTGTCATTCATCATTCTGGCGGTAAAGCGGTGCTAGCTCATCCTGGGCGGTACAATCTTAGTGCTAAATGGCTGAAAAGATTGGTTGCGCATTTTGCCGAACACCACGGTGACGCGATGGAAGTCGCGCAGTGCCAGCAATCGCCCAATGAACGTACCCAGCTGGCGGCCCTTGCGCGTCAGCATCATTTATGGGCATca                                                               <  2:278525/251‑1 (MQ=255)                                                                                                                                                               aaaaCCGGATACGTTCCGCCACAGTGGTGTACAATAGAACAAGCTATTGATGTCATTCATCATTCTGGCGGTAAAGCGGTGCTAGCTCATCCTGGGCGGTACAATCTTAGTGCTAAATGGCTGAAAAGATTGGTTGCGCATTTTGCCGAACACCACGGTGACGCGATGGAAGTCGCGCAGTGCCAGCACTCGCCCAATGAAAGTACCCAGCTGGCGGCCCTTGCGCGTCAGCATCATTTATGGGCATcac                                                              >  1:226995/1‑250 (MQ=255)                                                                                                                                                                                   acaGTGGTGTACAATAGAACAAGCTATTGATGTCATTCATCATTCTGGCGGTAAAGCGGTGCTAGCTCATCCTGGGCGGTACAATCTTAGTGCTAAATGGCTGAAAAGATTGGTTGCGCATTTTGCCGAACACCACGGTGACGCGATGGAAGTCGCGCAGTGCCAGCAATCGCCCAATGAACGTACCCAGCTGGCGGCCCTTGCGCGTCAGCATCATTTATGGGCATCACAAGGATCTGAGTTTCATCAGc                                         <  2:229051/251‑1 (MQ=255)                                                                                                                                                                                     aGTGGTGTACAATAGAACAAGCTATTGATGTCATTCATCATTCTGGCGGTAAAGCGGTGCTAGCTCATCCTGGGCGGTACAATCTTAGTGCTAAATGGCTGAAAAGATTGGTTGCGCATTTTGCCGAACACCCCGGTGACGCGATGGAAGTTGCGCAGTGCCAGCAATCTCCCAATGAACGTACCCAGCTGGCGGCCCTTGCGCGTCAGCATCATTTATGGGCATCACAAGGATCTGATTTTCATCAGcct                                       <  2:59313/251‑2 (MQ=255)                                                                                                                                                                                             aCAATAGCAAAAGCTATTGATGTCATTCATCATTCTGGCGGTAAAGCGGTGCTAGCTCATCCTGGGCGGTACAATCTTAGTGCTAAATGGCTGAAAAGCTTGGTTGCGCATTTTGCCGAACACCACGGTGACGCGATGGAAGTCGCGCAGTGCCAGCAATCGCCCAATGAACGTACCCAGCTGGCGGCCCTTGCGCGTCAGCATCATTTATGGGCCTCACAAGGATCTGATTTTCATCAGCCATGCCCGTg                               >  2:434587/1‑251 (MQ=255)                                                                                                                                                                                             aCAATAGAACAAGCTATTGATGTCATTCATCATTCTGGCGGAAAAGCGGTGCTAGCTCATCCTGGGCGGTACAATCTTAGTGCTAACTGGCTGAAAAGATTGGTTGCGCATTTTGCCGAACACCACGGTGACGCGATGGAAGTCGCGCAGTGCCAGCAATCGCCCAATGACCGTACCCAGCTGGCGGCCCTTGCGCGTCAGCATCATTTATGGGCATCACAAGGATATGATTTTCATCAGCCATGCCCGTg                               >  1:297651/1‑251 (MQ=255)                                                                                                                                                                                                       aaGCTATTGATGTCATTCATCATTCTGGCGGTAAAGCGGTGCTAGCTCATCCTGGGCGGTACAATCTTAGTGCTAAATGGCTGAAAAGATTGGTTGCGCATTTTGCCGAACACCACGGTGACGCGATGGAAGTCGCGCAGTGCCAGCAATCGCCCAATGAACGTACCCAGCTGGCGGCCCTTGCGCGTCAGCATCATTTATGGGCATCCCAAGGATCTGATTTTCATCAGCCATGCCCGGGGATCGAACTg                     >  2:112813/1‑251 (MQ=255)                                                                                                                                                                                                                  gTCATTCAGCATTCTGGCGGTAAAGCGGTGCTAGCTCATCCTGGTCGGTACAATCTTAGTGCTAAAGTGCTGAAAAGATTGGTTGCGCATTTTGCCGAACACCACGGTGACGCGATGGAAGTCGCGCAGTGCCAGCAATCGCCCAAGGAACGTACCCAGCGGGCTGCCCTTGCGCGTCAGCATCATGTATGGGCATCACAAGGATCTGATTTTCATCAGCCATGCCCGTGGATCGAACTGGGTCGTAAAc           <  2:386936/250‑1 (MQ=255)                                                                                                                                                                                                                    cattcatCATTCTGGCGGTAAAGCGGTGCTAGCTCATCCTGGGCGGTACAATCTTAGTGCTAAATGGCTGAAAAGATTGGTTGCGCATTTTGCCGAACACCACGGTGACGCGATGGAAGTCGCGCAGTGCCAGCAATCGCCCAATGAACGTACCCAGCTGGCGGCCCTTGCGCGTCAGCATCATTTATGGGCATCACAAGGATCTGATTTTCATCAGCCATGCCCGTGGATCGAACTGGGTCGTAAActct        >  1:260932/1‑251 (MQ=255)                                                                                                                                                                                                                           cattcatgCGGTAAAGCGGGGCTAGCTCATCCTGGGCGGTACAATCTTAGTGCTAAATGGCTGAAAAGATTGGTTGCGCAGTTTGCCGAACACCACGGTGACGCGATGGAAGTCGCGCAGTTCCAGCAATCGCCCAATGAACGTACCCAGCTGGCGGCCCTTGCGCGTCAGCATCATTTATGGGCAGCACAAGGATTGGATTTTCATCAGCCATGCCCGTTGATCGAACCGGGTCGTAAACTCTGGCTGc  <  2:450612/243‑1 (MQ=255)                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                            AAAGACTTGAAAAAGCGCAAATACCTGGGGCGCTGGAAGGCGCACAACGACTGGCGCAGGGCGGCGCAGTGACGCGCGGTCATTTTGCGCGTTTCCTTGTTGAGTGCGGCAAAGCCAGTTCAATGGCGGATGTCTTTAAAAAGTATCTGGCGCGCGGGAAAACCGGATACGTTCCGCCACAGTGGTGTACAATAGAACAAGCTATTGATGTCATTCATCATTCTGGCGGTAAGGCGGTGCTAGCTCATCCTGGGCGGTACAATCTTAGTGCTAAATGGCTGAAAAGATTGGTTGCGCATTTTGCCGAACACCACGGTGACGCGATGGAAGTCGCGCAGTGCCAGCAATCGCCCAATGAACGTACCCAGCTGGCGGCCCTTGCGCGTCAGCATCATTTATGGGCATCACAAGGATCTGATTTTCATCAGCCATGCCCGTGGATCGAACTGGGTCGTAAACTCTGGCTGC  >  NC_000913/1323566‑1324033

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑