Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,252,663	C→T	S533F (TCT→TTT)	dhaR →	DNA‑binding transcriptional dual regulator DhaR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,252,663	0	C	T	100.0%	95.0 / NA	33	S533F (TCT→TTT)	dhaR	DNA‑binding transcriptional dual regulator DhaR
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (16/17);  total (16/17)

GCGTTTAATACCAATTGATGTCAAAGTGATTGCAACAACGACCGCGGACCTCGCAATGCTGGTGGAACAAAATCGTTTTAGTCGCCAGCTGTATTACGCGCTGCATGCATTTGAAATTACCATCCCGCCTCTGCGTATGCGGCGTGGCAGCATTCCGGCGCTGGTGAATAACAAATTACGCAGTCTTGAAAAACGCTTCTCTACGCGGCTGAAAATTGATGACGATGCCCTCGCTCGCCTGGTTTCTTGTGCATGGCCAGGCAACGATTTTGAACTTTACAGCGTCATCGAGAATCTTGCTCTGAGTAGTGATAACGGGCGCATTCGCGTCAGTGATTTGCCGGAACATCTGTTTACCGAGCAGGCGACAGATGATGTCAGCGCCACCCGCCTTTCCACCAGTCTGTCATTTGCGGAAGTTGAAAAAGAGGCAATTATTAACGCAGCCCAGGTCACAGGCGGTCGCATTCAGGAAATGTCGG  >  NC_000913/1252418‑1252899                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                             gcgTTTAATACCAATTGATGTCAAAGTGATTGCAACAACGAACGCGGACCTCGCAATGCTGGTGGAACAAAATCGTTTTAGTCGCCAGCTGTATTACGCGCTGCATGCATTTGAAATTACCATCCCGCCTCTGCGTATGCGGCGTGGCAGCATTCCGGCGCTGGTGAATAACAAATTACGCAGTCTTGAAAAACGCTTCTCTACGCGGCTGAAAATTGATGAAGATGCCCTCGCTCGACTGGTTTTTtgtg                                                                                                                                                                                                                                         >  1:370941/1‑251 (MQ=255)       aaTACCAATTGATGTCAAAGTGATTGCAACAACGACCGCGGACCTCGCAATGCTGGTGGAACAAAATCGTTTTAGACGCCAGCTGTATTACGCGCTGCATGCATTTGAAATTACCATCCCGCCTCTGCGTATGCGGCGTGGCAGCATTCCGGCGCTGGTGAATAACAAATTACGCAGTCTTGAAAAACGCTTCTCTACGCGGCTGAAAATTGATGACGATGCCCTCGCTCGCCTGGTTTTTTGTGCTTGGc                                                                                                                                                                                                                                   >  1:311245/1‑251 (MQ=255)                         aGTGTTTGCAAAAACGACCGTGGACCTCGAAAGGTTGGTGGAACAAATGTTTTTTAGTCCCCAGCTGTATTCCGGGCTGCACGCTTTTAAAATTACCATCCCGCCTCTGCGTAGGCGGCGTGGCAGCATTCCGGCGCTGGTGAATAACAAATTACGCAGTCTTGAAAAACGCTTCTCTACGCGGCTGAAAATTGATGACGATGCCCTCGCTCGCCTGGTTTTTTGTGCATGGCCAGGCAACGATTTTGAAc                                                                                                                                                                                                                 <  1:329873/251‑1 (MQ=255)                                                                           gTTTTAGTCGCCAGCTGTATTACGCGCTGCATGCATTTGAAATTACCATCCCGCCTCTGCGTATGCGGCGTGGCAGCATTCCGGCGCTGGTGAATAACCAATTACGCAGTCTTGAAAAACGCTTCTCTACGCGGCTGAAAATTGATGACGATGCCCTCGCTCGCCTGGTTTTTTGTGCAt                                                                                                                                                                                                                                      >  2:395471/1‑180 (MQ=255)                                                                                gggcccccgCTGTATCACGGGCTGCATGCATTTGAAATTACCACCCCGCCTCTGCGTATGCGGCGTGGCAGCATTCCGGCGCTGGTGAATAACAAATTACGCAGTCTTGAAAAACGCTTCTCTACGCGGCTGAAAATTGATGACGATGCCCTCGCTCGCCTGGTTTTTTGTGCATGGCCAGGCAACGATTTTGAACTTTACAGCGTCATCGAGAATCTTGCTCTGAGTAGTGATAACGGGCGCATTCGCGt                                                                                                                                                          <  2:235106/243‑1 (MQ=255)                                                                                         cTGTATTACGCGCTGCATGCATTTGAAATTACCATCCCGCCTCTGCGTATGCGGCGTGGCAGCATTCCGGCGCTGGTGAATAACACATTACGCAGTCTTGAAAAACGCTTCTCTACGCGGCTGAAAATTGATGACGATGCCCTCGCTCGCCTGGTTTTTTGTGCATGGCCAGGCAACGATTTTGACCTTTACAGCGTCATCGAGAATCTTGCTCTGAGTAGTGATAACGGGCGCATTCGCGTCAGTGAttt                                                                                                                                                 >  2:481788/1‑251 (MQ=255)                                                                                                   cgcTGCATGCATTTGAAATTACCATCCCGCCTCTGCGTATGCGGCGTGGCAGCATTCCGGCGCTGGTGAATAACAAATTACGCAGTCTTGAAAAACGCTTCTCTACGCGGCTGAAAATTGATGACGATGCCCTCGCTCGCCTGGTTTTTTGTGCATGGCCAGGCAACGATTTTGAACTTTCCAGCGTCATc                                                                                                                                                                                                   >  2:350556/1‑191 (MQ=255)                                                                                                      tgcatgcaTTTGAAATTACCATCCCGCCTCTGCGTATGCGGCGTGGCAGCATTCCGGCGCTGGTGAATAACAAATTACGCAGTCTTGAAAAACGCTTCTCTACGCGGCTGAAAATTGATGACGATGCCCTCGCTCGCCTGGTTTTTTGTGCATGGCCAGGCAACGATTTTGAACTTTACAGCGTCATCGAGAATCTTGCTCTGAGTAGTGATAACGGGCGCATTCGCGTCAGTGATTTGCCGGAACATCTg                                                                                                                                    >  1:134352/1‑251 (MQ=255)                                                                                                       gcatgcatGTGAAATGACCATACCGACTCTGCGTATGCGGCGTGGAAGCATTCCGGCTATGGTGAATAACAAATTAAGCAGTCTTGAAAAACGCTTCTCTACGCGGCTGAAAATTGATGACGATGCCCTCGCTCGCCTGGTTTTTTGTGCATGGCCAGGCAACTGTTTTGAACTTTACAGTGTCCTCGAGAATCTTGATCTGAGTAGTGAAAACGGGCGCATTCGAGTCAATGATTTGCCGGAACATCtat                                                                                                                                   >  2:73688/1‑249 (MQ=255)                                                                                                         atgcatTTGAAATTACCATCCCGCCTCTGCGTATGCGGCGTGGCAGCATTCCGGCGCTGGTGAATAACAAATTACGCAGTCTTGAAAAACGCTTCTCTACGCGGCTGAAAATTGATGACGATGCCCTCGCTCGCCTGGTTTTTTGTGCATGGCCAGGCAACGATTTTGAACTTTACTGCGTCATCGAGAATCTTGCTCTGAGTAGTGTTAACGGGCGCATTCGCGTCAGTGCTTTTCCGGacactctgttt                                                                                                                                 >  2:101171/1‑241 (MQ=255)                                                                                                              tttGAATTTACCACCCCGCCTCTGCGTATGCGGCGTGGCAGCATTCCGGCGCTGGTGAATAACAAATTACGCAGTCTTGAAAAACGCTTCTCTACGCGGCTGAAAATTGATGACGATGCCCTCGCTCGCCTGGTTTTTTGTGCATGGCCAGGCAACGATTTTGAACTTTACAGCGTCATCGAGAATCTTGCTCTGAGTAGTGATAACGGGCGCATTCGCGTCAGTGATTTGCCGGAACATCTGTTTAcc                                                                                                                              <  1:348937/249‑1 (MQ=255)                                                                                                                                     gCGTATGCGGCGTGTCAGCATTCCGGCGATGGTGAATAACAAATTACTCATTCTTGAAAAACGCTTCGCTACGCGGCTGAAAAATGATGACGATGCCCTCGCTCGCCTGGTTTTTTGTGCATGGCCAGGCAACGATTTTGAACTTTACAGCGTCATCGAGAATCTTGCTCTGAGTAGTGATAACGGGCGCATTCGCGTCAGTGATTTGCCGGAACATCTGTTTACCGAGCAGGCGACAGATGATGTCa                                                                                                        <  2:160397/248‑1 (MQ=255)                                                                                                                                         aTGCGGCGTGGCATCATTCCGGCGCTGGTGAATAACAAATTACGCAGTCTTGAAAAACGCTTCTCTACGCGGCTGAAAATTGATGACGATGCCCTCGCTCGCCTGGTTTTTTGTGCATGGCCAGGCAACGATTTTGAACTTTACAGCGTCATCGAGAATCTTGCTCTGAGTAGTGATAACGGGCGCATTCGCGTCAGTGATTTGCCGGAACATCTGTTTACCGAGCAGGCGACAGATGATGTCAGCGCCa                                                                                                  <  1:283535/250‑1 (MQ=255)                                                                                                                                             ggcgTGGCAGCATTCCGGCGCTGGTGAATAACAAATTACGCAGTCTTGAAAAACGCTTCTCTACGCGGCTGAAAATTGATGACGATGCCCTCGCTCGCCTGGTTTTTTGTGCATGGCCAGGCAACGATTTTGAACTTTACAGCGTCATCGAGAATCTTGCTCTGAGTAGTGATAACGGGCGCATTCGCGTCAGTGATTTGCCGGAACATCTGTTTACCGAGCAGGCGACAGATGATGTCAGCGCCACCCGc                                                                                             >  1:92794/1‑251 (MQ=255)                                                                                                                                                  ggCAGCATTCCGGCGCTGGTGAATAACAAATTACGCAGTCTTGAAAAACGCTTCTCTACGCGGCTGAAAATTGATGACGATGCCCTCGCTCGCCTGGTTTTTTGTGCATGGCCAGGCAACGATTTTGAACTTTACAGCGTCATCGAGAATCTTGCTCTGAGTAGTGATAACGGGCGCATTCGCGTCAGTGATTTGCCGGAACATCTGTTTACCGAGCAGGCGACAGATGATGTCAGCGCCACCCGCCTTTc                                                                                        <  1:224161/251‑1 (MQ=255)                                                                                                                                                               cgcTGGTGAATAACAAATTACGCAGTCTTGAAAAACGCTTCTCTACGCGGCTGAAAATTGATGACGATGCCCTCGCTCGCCTGGTTTTTTGTGCATGGCCAGGCAACGATTTTGAACTTTCCAGCGTCATCGAGAATCTTGCTCTGATTAGAGATAAAGGGCGCATTCGCGTCAGTGATTTGCCGGTGAATCTGTTTACCGAGCAGGTGACAGATGGAGTCAGAGCCACCCGCCTTTCCACCAGTCTGTc                                                                            >  2:324139/1‑250 (MQ=255)                                                                                                                                                               cgcTGGTGAATAACAAATCACGCAGTCTTTAAAAACGCTCCTCTACGCTGCTGAAAATTGATTACGATTCCCTCGCTCGCCTGGTTGTGTGTGCATGGCCAGGCAACGATTTTGAACTTTACAGCGTCATCGAGAACCTGGCTCTTAGTAGTGATAACGGGCGCATTCGCGGCCGTGATTTGCCGGAACATCTGTTTACCGAGCAGGCGACAGATGATGTCAGCGCCACCCGCCTTTCCACCAGTCTGTCa                                                                           <  2:370941/251‑1 (MQ=255)                                                                                                                                                                gcTGGTGAATAACAAATTACGCAGTCTTGAAAAACGCTTCTCTACGCGGCTGAAAATTGATGACGATGCCCTCGCTCGCCTGGTTTTTTGTGCATGGCCAGGCAACGATTTTGAACTTTACAGCGTCATCGAGAATCTTGCTCTGAGTAGTGATAACGGGCGCATTCGCGTCAGTGATTTGCCGGAACATCTGTTTACCGAGCAGGCGACAGATGATGTCAGCGCCACCCGCCTTTCCACCAGTCTGTCAt                                                                          <  1:380602/251‑1 (MQ=255)                                                                                                                                                                gcTGGTGAATAACAAATTACGCAGTCTTGAAAAACGCTTCGCTACGCGGCTGAAAATTGATGACGATGCCCTCGCTCGCCTGGTTTTTTGTGCATGGCCAGGCAACGATTTTGAACTTTACAGCGTCATCGAGAATCTTGCTCTGAGTAGTGATAACGGGCGCATTCGCGTCAGTGATTTGCCGGAACATCTGTTTACCGAGCAGGCGACAGATGATGTCAGCGCCACCCGCCTTTCCACCAGTCTGTCAt                                                                          <  2:134352/251‑1 (MQ=255)                                                                                                                                                                 cTGGTGAATAACAAATTACGCAGTCTTGAAAAACGCTTCTCTACGCGGCTGAAAATTGATGACGATGCCCTCGCTCGCCTGGTTTTTTGTGCATGGCCAGGCAACGATTTTGAACTTTACAGCGTc                                                                                                                                                                                                      >  2:125328/1‑126 (MQ=255)                                                                                                                                                                 cTGGTGAATAACAAATTACGCAGTCTTGAAAAACGCTTCTCTACGCGGCTGAAAATTGATGACGATGCCCTCGCTCGCCTGGTTTTTTGTGCATGGCCAGGCAACGAGTTTGAACTTTACAGCGTc                                                                                                                                                                                                      <  1:125328/126‑1 (MQ=255)                                                                                                                                                                   ggTGAATAACAAATTACGCAGTCTTTAAAAACGCTTCTCTACGCGGTTGAAAATTGATGACGATGCTCTCGCTCGCCTGGTTTTTTGTGCATGGCCAGGCAACGATTTTGAACTTTACAGCGTCATCGAGAATCTTGCTCTGAGTAGTGATAACGGGCGCATTCGCGTCAGTGATTTGCCGGAACATCTGTTTACCGAGCAGGCGACAGATGATGTCATCGCCACCCGCCTTTCCACCAGTCTGTCAttt                                                                        <  1:404979/250‑1 (MQ=255)                                                                                                                                                                            ccaaTTAGGCAGCCTGGAAAACCTCTTCTTTACCCGGCTGAAAATTGATGACGATGCCCTCTCTCGCCTGGTTTTTTGTGCATGGCCAGGCAACGAGTTTGAACTTTACAGCGTCATCGAGAATCTTGCTATGAGTAGTGATAACGGGCGCATTCGCGTCAGTGATTTGCCGGAACATCTGTTTATCGAGCAGGAGAAAGATGATGTCAGCGCCACCCGCCTTTCCACCAGTCTGTCATTTGCGGAAGTTg                                                              <  2:311245/249‑1 (MQ=255)                                                                                                                                                                              aaTTACGCAGTCTTGAAAAACGCTTCTCTACGCGGCTGAAAATTGATGACGATGCCCTCGCTCGCCTGGTTTTTTGTGCATGGCCAGGCAACGATTTTGAACTTTACAGCGTCATCGAGAATCTTGCTCTGAGTAGTGATAACGGGCGCATTCGCGTCAGTGATTTGCCGGAACATCTGTTTACCGAGCAGGCGACAGATGATGTCAGCGCCACCCGCCTTTCCACCAGTCTGTCATTTGCGGACGTTGa                                                             >  1:388955/1‑250 (MQ=255)                                                                                                                                                                                            gAAAAACGCTTCTCTACGCGGCTGAAAATTGATGACGATGCCCTCGCTCGCCTGGTTTTTTGTGCATGGCCAGGCAACGATTTTGAACTTTCCAGCGTCATCGAGAATCTTGCTCTGAGTAGTGATAACGGGCGCATTCGCGTCAGTGATTTGcc                                                                                                                                              >  2:462537/1‑155 (MQ=255)                                                                                                                                                                                            gAAAAACGCTTCTCTACGCGGCTGAAAATTGATGACGATGCCCTCGCTCGCCTGGTTTTTTGTGCATGGCCAGGCAACGATTTTGAACTTTACAGCGTCATCGAGAATCTTGCTCTGAGTAGTGATAACGGGCGCATTCGCGTCAGTGATTTGcc                                                                                                                                              <  1:462537/155‑1 (MQ=255)                                                                                                                                                                                                  cGCTTCTCTACGGGGCTGAAAATTGATGACGATGCCCTCGCTCGCCTGGTTTTTTGTGCATGGCAAGGCAACGATTTTGAACTTTACAGCGTCATCGAGAATCTTGCTCTGAGTAGTGATAACGGGCGCATTCGCGTCATTGATTTGCCGGAACATCTGGTTACCGAGCAGGCGACAGATGATGTCAGCGCCACCCGCCTTTCCACAAGTCTGTCATTTGCGGAAGTTGAAAAAGAGGCAATTATTAACGc                                        <  1:394864/251‑1 (MQ=255)                                                                                                                                                                                                      tctcTACGCGGCTGAAAATTGATGACGATGCCCTCGCTCGCCTGGTTTTTTGTGCATGGCCAGGCAACGATTTTGAACTTTACAGCGTCATCGAGAATCTTGCTCTGAGTAGTGATAACGGGCGCATTCGCGTCAGTGATTTGCCGGAACATCTGTTTACCGAGCAGGCGACAGATGATGTCAGCGCCACCCGCCTTTCCACCAGTCTGTCATTTGCGGAAGTTGAAAAAGAGGCAATTATTAACGCAGc                                     <  1:347163/250‑1 (MQ=255)                                                                                                                                                                                                         ctACGCGGCTGAAACTTGATGACGATGCCCTAGCTCGCCTGGTTTTTTGTGCATGGCAAGGCAACGATTTTGAACTTTCCAGCGTCATCGAGAATCTTGCTCTGAGTAGTGATAACGGGCGCATTCGCGTCAGTGATTTGCCGGAACATCTGTTTACCGAGCAGGCGGCAGCTGATGTCCGCGCCACCCGCCTTTCCACCAGTCTGTCATTTGCGGAAGTTGGAACAGAGGCAATTGTTAACGcctccc                                   >  2:69388/1‑244 (MQ=255)                                                                                                                                                                                                                           atgaCGATGCCCTCGCTCGCCTGGTTTTTTGTGCATGGCCAGGCAACGATTTTGAACTTTACAGCGTCATCGAGAATCTTGCTCTGAGTAGTGATAACGGGCGCATTCGCGTCAGTGATTTGCCGGAACATCTGTTTACCGAGCAGGCGACAGATGATGTCAGCGCCACCCGCCTTTCCACCAGTCTGTCATTTGCGGAAGTTGAAAAAGAGGCAATTATTAACGCCGCCCAGGTCACAggagggagcatt               >  2:318317/1‑241 (MQ=255)                                                                                                                                                                                                                           aagaCGATGCACTAGCTCCCCTGGTTTTTTGTGCATGGCCAGGCAACGATTTTGCACTTTACAGCGTCATCGAGAATCTTGCTCTGAGTAGAGATAACGGAAGCATTCGCGTCAGTGATTTGCCGGAACATCTGTTTACCGAGCAGGCGACAGATGATGTCAGCGCCACCCGCCTTTCCACCAGGTTGTCATTTTCGGTGGTTGAAAAAGAGGAAATTATTCACGCAGCAGAGATCACAGGAGGtagcct                >  2:309242/3‑245 (MQ=255)                                                                                                                                                                                                                                    cccTCGCTCGCCTGGTTTTTGGTGCATGGCCAGGCATCGATTTTGAACTTTACAGCGTCACCGAGAATCTTGCTCGGAGTAGTGATAACGGGCGCATTCGCGTCAGTGATTTGCCGGAACATCTGTTTGCCGAGCAGGCGACAGATGATGTCAGCGCCTCCCGCCTTTCCACCAGTCTGTCATTTGCGGAAGTTGAAAAAGAGGCAATTATTAACGCAGCCCAGGTCACAGGCGGTCGCATTCAGGAAATg      <  2:7316/251‑1 (MQ=255)                                                                                                                                                                                                                                        cgctcgcCTGGTTTTTTGTGCATGGCCAGGCAACGATTTTGAACTTTACAGCGTCATCGAGAATCTTGCTCTGAGTAGTGATATCGGGCGCATTCGCGTCAGTGATTGGCCGGAACATCTGTTTACCGAGCAGGCGACAGATGATGTCAGCGCCACCCGCCTTTCCACCAGTCTGTCATTTGCGGAAGTTGAAAAAGAGGCAATTATTAACGCAGCCCAGGTCACAGGCGGTCGCATTCAGGAAATGTCgg  <  1:101171/251‑1 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                             GCGTTTAATACCAATTGATGTCAAAGTGATTGCAACAACGACCGCGGACCTCGCAATGCTGGTGGAACAAAATCGTTTTAGTCGCCAGCTGTATTACGCGCTGCATGCATTTGAAATTACCATCCCGCCTCTGCGTATGCGGCGTGGCAGCATTCCGGCGCTGGTGAATAACAAATTACGCAGTCTTGAAAAACGCTTCTCTACGCGGCTGAAAATTGATGACGATGCCCTCGCTCGCCTGGTTTCTTGTGCATGGCCAGGCAACGATTTTGAACTTTACAGCGTCATCGAGAATCTTGCTCTGAGTAGTGATAACGGGCGCATTCGCGTCAGTGATTTGCCGGAACATCTGTTTACCGAGCAGGCGACAGATGATGTCAGCGCCACCCGCCTTTCCACCAGTCTGTCATTTGCGGAAGTTGAAAAAGAGGCAATTATTAACGCAGCCCAGGTCACAGGCGGTCGCATTCAGGAAATGTCGG  >  NC_000913/1252418‑1252899

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑