Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,244,908	C→T	L61L (CTG→TTG)	ymgE →	PF04226 family protein YmgE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,244,908	0	C	T	100.0%	75.4 / NA	26	L61L (CTG→TTG)	ymgE	PF04226 family protein YmgE
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (12/14);  total (12/14)

AGAGCGTTGATGTAAGCATTTCTTGCGTCCGATTCATCGAAACGAGGGCCTGAACATGGGAATTATTGCCTGGATTATTTTTGACCTGATAGCCGGCATTATCGCCAAGCTAATCATGCCGGGGCGTGATGGTGGTGGATTTTTCCTGACCTGTATTCTCGGGATAGTCGGTGCGGTGGTCGGCGGCTGGCTGGCGACCATGTTTGGCATTGGCGGCTCCATCAGTGGTTTTAATCTGCACAGCTTCCTGGTGGCGGTGGTGGGAGCTATTCTCGTTCTGGGCATATTCCGCCTCCTGCGAAGAGAATAAGATTTTCATAAGGCGGATAGCGATACAGATGCCGC‑TATCCGCTTTCACATCAGAACGTATACTCGACACCTGCTTTACGGGTGAAAAAAATCAATATAGCACTTCGATATCCGATAAAGTCGGTTCCCGGTAACAACAACAGAATCCTTTTTTAATTATTGTTTCGTTGTT  >  NC_000913/1244673‑1245153                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                       agagCGTTGATGTAAGCATTTCTTGCGTCCGATTCATCGAAACGAGGGCCTGAACATGGGAATTATTGCCTGGATTATTTTTGACCTGATAGCCGGCATTATCGCCAAGCTAATCATGCCGGGGCGTGATGGTGGTGGATTTTTCCTGACCTGTATTCTCGGGATAGTCGGTGCGGTGGTCGGCGGCTGGCTGGCGACCATGTTTGGCATTGGCGGCTCCATCAGTGGTTTTAATTTGCACAGCTTCCtg                                                                                                                                                                                                                                          <  2:170355/250‑1 (MQ=255)   ggCGTTGATGTAAGCATTTCTTGCGTCCGATTCATCGAAACGCGGGCCTGAACATGGGAATTGTTGCCTGGATTATTTTTGACCTGATAGCCGGCATTATCTCCAAGCTAATCATGCCGGGGCGTGATGGTGGTGGATTTTTCCTGACCTGTATTCTCGGGATAGTCGGTGCGGTGGTCGGCGGCTGGCTGGCGACCATGTTTGGCATTGGCGGCTCCATCAGTGGTTTTAATTTGCACAGCTTCCtggtg                                                                                                                                                                                                                                       <  2:225274/250‑1 (MQ=255)    gCGTTGATGTAAGCATTTCTTGCGTCCGATTCATCGAAACGAGGGCCTGAACATGGGAATTATTGCCTGGATTATTTTTGACCTGATAGCCGGCATTATCGCCAAGCTAATCATGCCGGGGCGTGATGGTGGTGGATTTTTCCTGACCTGTATTCTCGGGATAGTCGGTGCGGTGGTCGGCGGCTGGCTGGCGACCATGTTTGGCATTTGCGGGCCCCTCCGGGGTTTTTATTTTGACAGCTTCCtggtgg                                                                                                                                                                                                                                      >  2:386815/1‑251 (MQ=255)          aTGTAAGCATTTCTTGCGTCCGATTCATCGAAACGAGGGCCTGAACATGGGAATTATTGCCTGGATTATTTTTGACCTGATAGCCGGCATTATCGCCAAGCTAATCATGCCGGGGCGTGATGGTGGTGGATTTTTCCTGACCTGTATTCTCGGGATAGTCGGTGCGGTGGTCGGCGGCTGGCTGGCGACCATGTTTGGCATTGGCGGCTCCATCAGTGGGTTTAATTTGCACCGCTTCCTGGGGGCggggg                                                                                                                                                                                                                                >  1:251922/1‑248 (MQ=255)                        tGCGTCCGATGCATCGAAACGAGGGCCTGAACATGGGAATTATTGCCTGGATTATTTTTGACCTGATAGCCGGCATTATCGCCAAGCTAATCATGCCGGGGCGTGATGGTGGTGGATTTTTCCTGACCTGTATTCTCGGGATAGTCGGTGCGGTGGTCGGCGGCTGGCTGGCGACCATGTTTGGCATTGGCGGCTCCATCAGTGGTTTTAATTTGCACAGCTTCCTGGTGGCGGTGGTGGGAGCTAtt                                                                                                                                                                                                                     <  2:423721/248‑1 (MQ=255)                          cGTCCGATTCATCGAAACGAGGGCCTGAACATGGGAATTATTGCCTGGATTATTTTTGACCTGATAGCCGGCATTATCGCCAAGCTAATCATGCCGGGGCGTGATGGTGGTGGATTTTTCCTGACCTGTATTCTCGGGATAGTCGGTGCGGTGGTCGGCGGCTGGCTGGCGACCATGTTTGGCATTGGCGGCTCCATCAGTGGTTTTAATTTGCACAGCTTCCTGGTGGCGGTGGTGGGGGCTATTCTCGt                                                                                                                                                                                                                >  1:171610/1‑251 (MQ=255)                           gTCCGATTCATCGAAACGAGGGCCTGAACATGGGAATTATTGCCTGGATTATTTTTGACCTGATAGCCGGCATTATCGCCAAGCTAATCATGCCGGGGCGTGATGGTGGTGGATTTTTCCTGACCTGTATTCTCGGGATAGTCGGTGCGGTGGTCGGCGGCTGGCTGGCGACCATGTTTGGCATTGGCGGCTCCCTCAGTGGTTTTAATTTGCACAGCTTCCTGGTGGCGGGGGGGGGAGCTATTCTCGtt                                                                                                                                                                                                               >  1:283490/1‑251 (MQ=255)                                                        aTGGGAATTATTGCCTGGATTATTTTTGACCTGATAGCCGGCATTATCGCCAAGCTAATCATGCCGGGGCGTGATGGTGGTGGATTTTTCCTGACCTGTATTATCGGGATAGTCGGTGCGGTGGGCGGCGGCTGGCTGGCGACCATGTTTGGCATTGGCGGCTCCATCAGTGGTTTTAATTTGCACAGCTTACTGGTGGCGGTGGGGGGGGCTATTTTCGTTTTGGGCATATTTCGCCTCCTGTGAagagg                                                                                                                                                                                  >  1:102053/1‑250 (MQ=255)                                                             aaTTATTGCCTGGATTTTTTTTTGCCTGATAGTCGGCATTCTCTACAAGCTAATCATGCCGTTGTTTGATGGTGGTTGATTTTTCCTGACCTGTATTCTCGGGATAGTCGGCGCGGTGTTCGGCGGCTGGCTGGCGACCATGTTTGGCATTTGCGGCTCCATCACTTTTTTTAATTTGCACAGCTTCCTCGTGGCGGCGGTTTGAGCTATTCTCGTTATGGGCATATTCCGCCTCCTGCGAAGAGAAtcat                                                                                                                                                                             <  2:377380/251‑4 (MQ=255)                                                                tattGCCTGGATTATTTTTGACCTGATAGCCGGCATTATCGCCAAGCTAATCATGCCGGGGCGTGATGGTGGTGGATTTTTCCTGACCTGTATTCTCGGGATAGTCGGTGCGGTGGTCGGCGGCTGGCTGGCGACCATGTTTGGCATTGGCGGCTCCATCAGTGGTTTTAATTTGCACAGCTTCCTGGTGGCGGGGGTGGGGGCTATTCTCGGTTTGGGCATATTTCCCCTCCTGCGGAGAGAATAAgtat                                                                                                                                                                          >  2:261643/1‑248 (MQ=255)                                                                   tGCCTGGATTATTTTTGACCTGATAGCCGGCATTATCGCCAAGCTAATCATGCCGGGGCGTGATGGTGGTGGATTTTTCCTGACCTGTATTCTCGGGATAGTCGGTGCGGTGGTCGGCGGCTGGCTGGCGACCATGTTTGGCATTGGCGGCTCCATCAGTGGTTTTAATTTGCACAGCTTCCTGGTGGCGGTGGTGGGAGCTATTCTCGTTCTGGGCATATTCCGCCTCCTGCGAAGAGAATAAGAtttt                                                                                                                                                                        <  1:386815/250‑1 (MQ=255)                                                                    gCCTGGATTATTTTTGACCTGAGAGCCGGCATTATCGCCAAGCTAATCATGCCGGGGCGTGATGGTGGTGGATTTTTCCTGACCTGTATTCTCGGGATAGTCGGTGCGGTGGTCGGCGGCTGGCTGGCGACCATGTTTGGCATTGGCGGCTCCATCAGTGGTTTTAATTTGCACAGCTTCCTGGTGGCGGTGGTGGGAGATATTCTCGTTCTGGGCAGATTCCGCCTCCTGCGAAGAGAATAAGATTTTCa                                                                                                                                                                      <  2:164383/251‑1 (MQ=255)                                                                     ccTGGATTATTTTTGACCTGATAGCCGGCATTATCGCCAAGCTAATCATGCCGGGGCGTGATGGTGGTGGATTTTTCCTGACCTGTATTCTCGGGATAGTCGGTGCGGTGGTCGGCGGCTGGCTGGCGACCATGTTTGGCATTGGCGGCTCCATCAGTGGTTTTAATTTGCACAGCTTCCTGGTGGCGGTGGTGGGAGCTATTCTCGTTCTGGGCATATTCCGCCTCCTGCGAAGAGAATAAGATTTTCAt                                                                                                                                                                     <  1:124047/251‑1 (MQ=255)                                                                                        gATAGCCGGCATTATCGCCAAGCTAATCATGCCGGGGCGTGATGGTGGTGGATTTTTCCTGACCTGTATTCTCGGGATAGTCGGTGCGGTGGTCGGCGGCTGGCTGGCGACCATGTTTGGCATTGGCGGCTCCATCAGTGGTTTTAATTTGCACAGCTTCCTGGTGGCGGTGGTGGGAGCTATTCTCGTTCTGGGCATATTCCGCCTCCTGCGAAGAGAATAAGATTTTCATAAGGCGGATAGCGATAc                                                                                                                                                    <  2:251922/249‑1 (MQ=255)                                                                                                        gCCAAGCTAATCATGCCGGGGCGTGATGGTGGTGGATTTTTCCTGACCTGTATTCTCGGGATAGTCGGTGCGGTGGTCGGCGGCTGGCTGGCGACCATGTTTGGCATTGGCGGCTCCATCAGTGGTTTTAATTTGca                                                                                                                                                                                                                                                    >  1:447755/1‑137 (MQ=255)                                                                                                        gCCAAGCTAATCATGCCGGGGCGTGATGGTGGTGGATTTTTCCTGACCTGTATTCTCGGGATAGTCGGTGCGGTGGTCGGCGGCTGGCTGGCGACCATGTTTGGCATTGGCGGCTCCATCAGTGGTTTTAATTTGca                                                                                                                                                                                                                                                    <  2:447755/137‑1 (MQ=255)                                                                                                            aGCTAATCATGCCGGGGCGTGATGGTGGTGGATTTTTCCTGACCTGTATTCTCGGGATAGTCGGTGCGGTGGTCGGCGGCTGGCTGGCGACCATGTTTGGCATTGGCGGCTCCATCAGTGGTTTTAATTTGCACAGCTTCCTGGTGGCGGTGGTGGGAGCTATTCTCGTTCTGGGCATATTCCGCCTCCTGCGAAGAGAATAAGATTTTCATAAGGCGGATAGCGATACAGATGCCGC‑TATCCGCTTTcac                                                                                                                             <  2:171610/251‑1 (MQ=255)                                                                                                                aaTCATGCCGGGGCGTGGTGGTGGTGGATTTTTCCTGACCTGTATTCTCGGGATAGTCGGTGCGGTGGTCGGCGGCTGGCTGGCTACCATGTTTGGCATTGGCGGCTCCATCAGGGGTTTTAATTTGCACAGCTTCCTGGTGGCGGTGGGGGGGGCTATTCTCGGTCTGGGCATTTTCCCCCTACTGCGCAAAGAATAAGATTTTCATAAGGCGGCTAGCGATACAGGTGCCGCGTACCCGCTT‑CACATc                                                                                                                          >  1:174227/1‑250 (MQ=255)                                                                                                                aaTCATGCCGGGGCGTGATGGTGGTGGATTTTTCCTGACCTGTATTCTCGGGAGAGGCGGTGCGGTGGTCGGCGGCTGGCTGGCGACCATGTTTGGCATTGGCGGCTCCATCAGTGGTTTTAATTTGCACAGCTTCCTGGTGGCGGTGGTGGGAGCTATTCTCGTTCTGGGCATATTCCGCCTCCTGCGAAGAGAATAAGATTTTCATAAGGCGGATAGCGATACAGATGCCGC‑TATCCGCTTTCACATc                                                                                                                          <  2:283490/250‑1 (MQ=255)                                                                                                                                 aTGGTGGTGGATTTTTCCTGACCTGTATTCTCGGGATAGTCGGTGCGGTGGTCGGCGGCTGGCTGGCGACCATGTTTGGCATTGGCGGCTCCATCAGTGGGTTTAATTTGCACAGCTTCCTGGTGGCGGTGGTGGGGGCTATTTTCGTTCTGGGCATATTTCGCCTCCTGCGAAGAGAATAAACTTTTCATAAGGCAGATAGCGATACAGATACCGC‑TATCCGCCTTCACATCAGAACGGATACACGacac                                                                                                        >  1:14210/1‑251 (MQ=255)                                                                                                                                       gtggATTTTTCCTGACCTGTATTCTCGGGATAGTCGGTGCGGTGGTCGGCGGCTGGCTGGCGACCATGTTTGGCATTGGCGGCTCCATCAGTGGTTTTAATTTGCACAGCTTCCTGGTGGCGGTGGTGGGAGCTATTCTCGTTCTGGGCATATTCCGCCTCCTGCGAAGAGAATAAGATTTTCATAAGGCGGATAGCGATACAGATGCCGC‑TATCCGCTTTCACATCAGAACGTATACTCGACACCTGCtt                                                                                                  >  1:439682/1‑251 (MQ=255)                                                                                                                                                                cGGGATAGTCGGTGCGGTGGTCGGCGGCTGGCTGGCGACCATGTTTGGCATTGGCGGCTCCATCAGGGGTTTTTATTTGCACAGCTTCCTGGTTGCGGGGGTGGGCGCTTTTCTCGTTGTTTGCATATTTCACCTTCTTGGCAAAGAATAAGATTTTCATTATTAGGGTTGGGATATCGATGGCGC‑TATCCGCATTTACAACAAAACGGATACTCGATACATGCTTTGCTGGTGGAAAAAATccatttaac                                                                         >  2:278935/1‑243 (MQ=255)                                                                                                                                                                                   gtCGGCGGCTGGCTGGCGACCATGTTTGGCATTGGCGGCTCCATCAGTGGTTTTAATTTGCACAGCTTCCTGGTGGCGGTGGTGGGAGCTATTCTCGTTCTGGGCATATTCCGCCTCCTGCGAAGAGAATAAGATTTTCATAAGGCGGAt                                                                                                                                                            <  1:336894/150‑1 (MQ=255)                                                                                                                                                                                                                          tCCATCAGTGGTTTTAATTTGCACAGCTTCCTGGTGGCGGTGGTGGGCGCTATTCTCGTTCTGGGCATATTCCGCCTCCTGCGAAGAGAATAAGTTTTTCATAAGGCGGCTAGCGATACAGATGCCGC‑TATCCGCTTTCACATCAGAACGTATACTCGACACCTGCTTTACGGGTGCAAAAAATCCATTTAACACTTCGATATCCGCTAAAGTCGGTTCCCGGTAACAACAAAAGAATCCTTTTTTCatt                >  2:289453/1‑250 (MQ=255)                                                                                                                                                                                                                                   ggTTTTAATTTGCACAGCTTCCTGGTGGCGGTGGTGGGAGCTATTCTCGTTCTGGGCATATTCCGCCTCCTGCGAAGAGAATAAGATTTTCATAAGGCGGATAGCGATACAGATGCCGC‑TATCCGCTTTCACATCAGAACGTATACTCGACACCTGCTTTACGGGTGAAAAAAATCAATATAGCACTTCGATATCCGATAAAGTCGGTTCCCGGTAACAACAACAGAATCCTTTTTTAATTATTGTTTCg       <  1:441852/250‑1 (MQ=255)                                                                                                                                                                                                                                        tAATTTGCACAGCTTCCTGGTGGCGGGGGTGGGAGCTATTCTCGTTCTGGGCATAGTCCGCCTCCTGCGAAGAGAATAATATTTTCAGAAGGCGGATAGCGATACAGAGTCCGC‑TATCCGCTTTCACATCATAACGTATACTCGACACCTGCTTTACGGGTGAAAAAAATCAATATAGCACTTCGATATCCGATAAAGTCGGTTCCCGGTAACAACAACAGAATCCTTTTTTAATTATTGTTTCgttgtt  <  2:174227/250‑1 (MQ=255)                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                       AGAGCGTTGATGTAAGCATTTCTTGCGTCCGATTCATCGAAACGAGGGCCTGAACATGGGAATTATTGCCTGGATTATTTTTGACCTGATAGCCGGCATTATCGCCAAGCTAATCATGCCGGGGCGTGATGGTGGTGGATTTTTCCTGACCTGTATTCTCGGGATAGTCGGTGCGGTGGTCGGCGGCTGGCTGGCGACCATGTTTGGCATTGGCGGCTCCATCAGTGGTTTTAATCTGCACAGCTTCCTGGTGGCGGTGGTGGGAGCTATTCTCGTTCTGGGCATATTCCGCCTCCTGCGAAGAGAATAAGATTTTCATAAGGCGGATAGCGATACAGATGCCGC‑TATCCGCTTTCACATCAGAACGTATACTCGACACCTGCTTTACGGGTGAAAAAAATCAATATAGCACTTCGATATCCGATAAAGTCGGTTCCCGGTAACAACAACAGAATCCTTTTTTAATTATTGTTTCGTTGTT  >  NC_000913/1244673‑1245153

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑