Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,227,656	G→A	intergenic (‑184/‑25)	pliG ← / → ycgL	inhibitor of g‑type lysozyme/PF05166 family protein YcgL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,227,656	0	G	A	100.0%	68.8 / NA	23	intergenic (‑184/‑25)	pliG/ycgL	inhibitor of g‑type lysozyme/PF05166 family protein YcgL
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (11/12);  total (11/12)

GGAGGTTAGCAGTGCAAGCAGCAATACAGCCTTCCTGATGCTCTTGATTTTCATTGGTAGTCTCCTGTTCAGAATGAGTACGATGAACTGTTATAATATAACAATCCCTAACGGGAGAATTCCAGTGGCTTTCCTGCGTTCGGTAAAATCACGTTGCGGCATTTTTGCCGGGGGGATTACTTATAAAGGGGGCATGGACTCAACAAACCGTAGAAATCTACAGCTGGCTGGCTTGTGGCGCGGGTTTCATTGATGTTAGCTTATGCCAAAACCTGGCATCTTAAAGAGTAAAAGTATGTTTTGTGTGATTTATCGAAGCAGCAAGCGTGACCAGACCTATTTATATGTCGAAAAAAAAGACGATTTTTCGCGTGTTCCTGAGGAACTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTGCCGCTGGATGGGCGGAAGAAACTGGTCAATGCCGATATTGAAAAGGTTAAACAGGC  >  NC_000913/1227419‑1227901                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                      ggAGGTTAGCAGTGCAAGCAGCAATTCAGCCTTCCTGATGCTCTTGATTTTCATTGGTAGTCTCCTGTTCAGAATGAGTACGATGAACTGTTATAATATAACAATCCCTAACGGGAGAATTCCAGTTGCTTTCCTGCGTTCGGTAAAATCACGTTGCGGCATTTTTGCCGGGGGGATTACTTATAAAGGGGGCATGGACTCAACAAACCGTAGAAATCTACAGCTGGCTGGCTTGTGACGCGGGTTTCAt                                                                                                                                                                                                                                           <  1:400310/250‑1 (MQ=255) ggAGGTTAGCAGTGCAAGCAGCAATACAGCCTTCCTGATGCTCTTGATTTTCATTGGTAGTCTCCTGTTCAGAATGAGTACGATGAACTGTTATAATATAACAATCCCTAACGGGAGAATTCCAGTGGCTTTCCTGCGTTCGGTAAAATCACGTTGCGGCATTTTTGCCGGGGGGATTACTTATAAAGGTGGCATGGACTCAACAAACCGTAGAAATCTACAGCTGGCTGGCTTGTGACGCGGGTTTCAtt                                                                                                                                                                                                                                          <  1:269493/251‑1 (MQ=255)                                     gATGCTCTTGATTTGCATTGGTAGTACCCTGTTCAGAATGAGTACGATGAACTGTTATAATATAACAATCCCTAACGGGAGAATTCCAGTGGCTTTCCTGCGTTCGGTAAAATCACGTTGCGGCATTTTTGCCGGGGGGATTACTTATAAAGGGGGCATGGACTCAACAAACCGTAGAAATCTACAGCTGGCTGGCTTGTGACGCGGGTTTCATTGATGTTAGCTTATGCCAAAACCTGGCAGCTTAAaga                                                                                                                                                                                                      <  2:100450/251‑1 (MQ=255)                                       ggCTCTTGATTTTCATTGGTAGTCTTCTGTTCAGAATGGGTACGATGAACTGTTATAATATAACAATCCCTAACGGGAGAATTCCAGTGGCTGTCCTGCGTTCGGTAAAATCACGTTGCGGCATTTTTGCCGGGGTGATTACGTATAAAGGGGGCATGGACTCAACAAACCGTAGAAATCTACAGCTGGCTGGCTTGTGACGCGGGTTTCATTGATGTTAGCTTATGCCAAAACCTGGCATCTTAAAGAGt                                                                                                                                                                                                    <  2:295707/250‑1 (MQ=255)                                        gCTCTTGATTTTCATTGGTAGTCTCCTGTTCAGAATGAGTACGATGAACTGTTATAATATAACAATCCCTAACGGGAGAATTCCAGTGGCTTTCCTGCGTTCGGTAAAATCACGTTGCGGCATTTTTGCCGGGGGGATTACTTATAAAGGGGGCATGGACTCAACAAACCGTAGAAATCTACAGCTGGCTGGCTTGTGACGCGGGTTTCATTGATGTTAGCTTATGCCAAAACCTGGCATCTTAAAGAGt                                                                                                                                                                                                    >  1:214928/1‑250 (MQ=255)                                                           aGTCTCCGGTTCAGAAGGAGTACGATGAACTGGTATAATATAACAATCCCTAACGGGAGAATGCCAGTGGCTTTCCTGCGTTCGGTAAAATCACGTTGCGGCATTTTTGCCGGTGGGATTACCTATAAAGGGGGCATGGACTCAACAAACCGTAGAAATCTACAGCTGGCGGGCTTGTGACGCGGGTTTCATTGATGGTAGCTTATGCCAAAATCTGGCATCGTAAAGAGTAAAAGTAAGTTTTGTGTGa                                                                                                                                                                                 <  2:247963/250‑1 (MQ=255)                                                                  tGTTCCGAATGAGTACGATGAACTGTTATAATATAACAATCCCTACCGGGAGAATTCCAGTGGCTTTCCTGCGTTCGGTAAAATCACGTTGCGGCATTTTTGCCGGGGGGATTACTTATAAAGGGGGCATGGACTCAACAAACCGTAGAAATCTACAGCTGGCTGGCTTGTGACGCGGGTTTCATTGATGTTAGCTTATGCCAAAACCTGGCATCTTAAAGAGTAAAAGTATGTTTTGTGTGATTTATCgg                                                                                                                                                                         >  2:407964/1‑250 (MQ=255)                                                                                                   taaCAATCCCTAACGGGAGAATTCCAGTGGCTTTCCTGCGTTCGGTAAAATCACGTTGCGGCATTTTTGCCGGGGGGATTACTTATAAAGGGGGCATGGACTCAACAAACCGTAGAAATCTACAGCTGGCTGGCTTGTGACGCGGGTTTCATTGATGTTAGCTTATGCCAAAACCTGGCATCTTAAAGAGTAAAAGTATGTTTTGTGTGATTTATCGAAGCAGCAAGCGTGACCAGACCTATTTATATGTc                                                                                                                                        >  1:419902/1‑251 (MQ=255)                                                                                                        aTCCCTAACGGGAGAATTCCAGTGGCTTTCCTGCGTTCAGTAAAATCACGTTGCGGCATTTTTGCCGGGGGGATTACTTATAAAGGGGGCATGGACTCAACAAACCGTAGAAATCTACAGCTGGCTGGCTTGTGACGCGGGTTTCATTGATGTTAGCTTATGCCAAAACCTGGCATCTTAAAGAGTAAAAGTATGTTTTGTGTGATTTATCGAAGCAGCAAGCGTGACCAGACCTATTTATATGTCGaaaa                                                                                                                                   <  1:325693/251‑1 (MQ=255)                                                                                                               aagggAGAATTCCAGTGGTGTTCCTGCGTTCGGTAAAATCACGTTGCGGCATTTTTGCCGGGGGGATTACTTATAAAGGGGGCATGGACTCAACAAACCGTAGAAATCTACAGCTGGCTGGCTTGTGACGCGGGTTTCATTGATGTTAGCTTATGCCAAAACCTGGCATCTTAAAGAGTAAAAGTATGTTTTGTGTGATTTATCGAAGCAGCAAGCGTGACCAGACCTATTTATATGTCGAAAAAAAAGAc                                                                                                                            <  2:201918/249‑1 (MQ=255)                                                                                                                       aTTCCAGTGGCTTTCCTGCGTTCGGTAAAATCACGTTGCGGCATTTTTGCCGGGGGGATTACTTATAAAGGGGGCATGGACTCAACAAACCGTAGAAATCTACAGCTGGCTGGCTTGTGACGCGGGTTTCATTGATGTTAGCTTATGCCAAAACCTGGCATCTTAAAGAGTAAAAGTATGTTTTGTGTGATTTATCGAAGCAGCAAGCGTGACCAGACCTATTTATATGTCg                                                                                                                                       <  1:425133/232‑1 (MQ=255)                                                                                                                       aTTCCAGTGGCTTTCCTGCGTTCGGTAAAATCACGTTGCGGCATTTTTGCCGGGGGGATTACTTATAAAGGGGGCATGGACTCAACAAACCGTAGAAATCTACAGCTGGCTGGCTTGTGACGCGGGTTTCATTGATGTTAGCTTATGCCAAAACCTGGCATCTTAAAGAGTAAAAGTATGTTTTGTGTGATTTATCGAAGCAGCAAGCGAGACCAGACCTATTTATATGTCg                                                                                                                                       >  2:425133/1‑232 (MQ=255)                                                                                                                                                      cACGTTGCGGCATTTTTGCCGGGGGGATTACTTATAAAGGGGGCATGGACTCAACAAACCGTAGAAATCTACAGCTGGCTGGCTTGTGACGCGGGTTTCATTGATGTTAGCTTATGCCAAAACCTGGCATCTTAAAGAGTAAAAGTATGTTTTGTGTGATTTATCGAAGCAGCAAGCGTGACCAGACCTATTTATATGTCGAAAAAAAAGACGATTTTTCGCGTGTTCCTGAGGAACTGATGAAAGGtttt                                                                                     >  1:333784/1‑251 (MQ=255)                                                                                                                                                                                               ggCATGGACTCAACAAACCGTAGAAATCTACAGCTGGCTGGCTTGTGACGCGGGTTTCATTGATGTTAGCTTATGCCAAAACCTGGCATCTTAAAGAGTAAAAGTATGTTTTGTGTGATTTATCGAAGCAGCAAGCGTGACCAGACCTATTTATATGTCGAAAAAAAAGACGATTTTTCGCGTGTTCCTGAGGAACTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTGCCGCTGGATGGGCg                                            >  2:392565/1‑251 (MQ=255)                                                                                                                                                                                                   tGGACTCAACAAACCGTAGAAATCTACAGCTGGCTGGCTTGTGACGCGGGTTTCATTGATGTTAGCTTATGCCAAAACCTGGCATCTTAAAGAGTAAAAGTATGTTTTGTGTGATTTATCGAAGCAGCAAGCGTGACCAGACCTATTTATATGTCGAAAAAAAAGACGATTTTTCGCGTGTTCCTGAGGAACTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTGCCGCTGGATGGGCGga                                          >  1:214576/1‑249 (MQ=255)                                                                                                                                                                                                             aaaCCGTAGAAATCTACAGCTGGCTGGCTTGTGACGCGGGTTTCATTGATGTTAGCTTATGCCAAAACCTGGCATCTTAAAGAGTAAAAGTATGTTTTGTGTGATTTATCGAAGCAGCAAGCGTGACCAGACCTATTTATATGTCGAAAAAAAAGACGATTTTTCGCGTGTTCCTGAGGAACTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTGCCGCTGGATGGGCGGAAGAAACTGGTc                               >  1:451458/1‑250 (MQ=255)                                                                                                                                                                                                                              aGCTGGCTGGCTTGTGACGCGGGTTTCATTGATGTTAGCTTATGCCAAAACCTGGCATCTTAAAGAGTAAAAGTATGTTTTGTGTGATTTATCGAAGCAGCAAGCGTGACCAGACCTATTTATATGTCGAAAAAAAAGACGATTTTTCGCGTGTTCCTGAGGAACTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTGCCGCTGGATGGGCGGAAGAAACTGGTCAATGCCGATATTGaaa               >  1:248752/1‑249 (MQ=255)                                                                                                                                                                                                                               gctggctgGCTTGTGACGCGGGTTTCATTGATGTTAGCTTATGCCAAAACCTGGCATCTTAAAGAGTAAAAGTATGTTTTGTGTGATTTATCGAAGCAGCAAGCGTGACCAGACCTATTTATATGTCGAAAAAAAAGACGATTTTTCGCGTGTTCCTGAGGAACTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTGCCGCTGGATGGGCGGAAGAAACTGGTCAATGCCGATATTGAAAAgg            <  1:123194/251‑1 (MQ=255)                                                                                                                                                                                                                                  ggctggctTGTGACGCGGGTTTCATTGATGTTAGCTTATGCCAAAACCTGGCATCTTAAAGAGTAAAAGTATGTTTTGTGTGATTTATCGAAGCAGCAAGCGTGACCAGACCTATTTATATGTCGAAAAAAAAGACGATTTTTCGCGTGTTCCTGAGGAACTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTGCCGCTGGATGGGCGGAAGAAACTGGTCAATGCCGATATTGAAAAGGtt          >  1:429402/1‑250 (MQ=255)                                                                                                                                                                                                                                      ggctTGGGACGCGGGTTTCATTGATGTTAGCTTATGCCAAAACCTGGCATCTTAAAGAGTAAAAGTAGGTTTTGTGTGATTTATCGAAGCAGCAAGCGTGACCAGACCTATTTATATGTCGAAAAAAAAGACGATTTTTCGCGTGTTCCTGAGGAACTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTGCCGCTGGATGGGCGGAAGAAACTGGTCAATGCCGATATTGAAAAGGTTAAACa     <  2:342712/251‑1 (MQ=255)                                                                                                                                                                                                                                       gcgtgtgACGCTGGTTTCATTGATGTTAGCTTATGCCAAAACCTGTCATCTTAAAGAGTAAAAGTATGTTTTGTGTGATTGATCGAAGCAGCAAGCGTGACCAGACCTATTTATATGTCGAAAAAAAAGACGATTCATCGTGTGTTCCTGAGGAACTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTGCCGCTGGATGGGCGGAAGAAACTGGTCAATGCCGATATTGAAAAGGTTAAACAg    <  1:54196/248‑1 (MQ=255)                                                                                                                                                                                                                                         atgtgACGCGGGTTTCATTGATGTTATCTTATGTCAAAAACTGGCATCTTAAAGAGTAAAAGTATGTTTTGTGTGCTTTATCGAAGCAGCAAGCGTGACTAGACATATTTATATGTCGCAAAACAAGACGAATTTTCGCGTGTTCCTGAGGAACTGATGAAAGGATTTGGTCAGCCTCAGTTAGCGATGATTCTGCCGCTGGATGGTAGGAATAAACTGGTCAAAGCGAATATTGAAACGGTTAAAAAGGc  >  2:338365/2‑251 (MQ=255)                                                                                                                                                                                                                                          tgtgACGCGGGTTTCATTGATGTTAGCTTATGCCAAAACCTGGCATCTTAAAGAGTAAAAGTATGTTTTGTGTGATTTATCGAAGCAGCAAGCGTGACCAGACCTATTTATATGTCGAAAAAAAAGACGATTTTTCGCGTGTTCCTGAGGAACTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTGCCGCTGGATGGGCGGAAGAAACTGGTCAATGCCGATATTGAAAAGGTTAAACAGGc  <  2:248752/250‑1 (MQ=255)                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                      GGAGGTTAGCAGTGCAAGCAGCAATACAGCCTTCCTGATGCTCTTGATTTTCATTGGTAGTCTCCTGTTCAGAATGAGTACGATGAACTGTTATAATATAACAATCCCTAACGGGAGAATTCCAGTGGCTTTCCTGCGTTCGGTAAAATCACGTTGCGGCATTTTTGCCGGGGGGATTACTTATAAAGGGGGCATGGACTCAACAAACCGTAGAAATCTACAGCTGGCTGGCTTGTGGCGCGGGTTTCATTGATGTTAGCTTATGCCAAAACCTGGCATCTTAAAGAGTAAAAGTATGTTTTGTGTGATTTATCGAAGCAGCAAGCGTGACCAGACCTATTTATATGTCGAAAAAAAAGACGATTTTTCGCGTGTTCCTGAGGAACTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTGCCGCTGGATGGGCGGAAGAAACTGGTCAATGCCGATATTGAAAAGGTTAAACAGGC  >  NC_000913/1227419‑1227901

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑