Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,176,602	C→T	P392P (CCC→CCT)	lolC →	lipoprotein release complex ‑ inner membrane subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,176,602	0	C	T	100.0%	157.8 / NA	48	P392P (CCC→CCT)	lolC	lipoprotein release complex ‑ inner membrane subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (26/22);  total (26/22)

CATGATGGTCTTTATGGTGCAAGGGGCCAGCGCCGGGATTATCGGTGCGATCCTCGGAGCGGCGCTTGGCGCCCTGCTTGCCAGCCAGTTAAATAATCTGATGCCGATAATCGGCGTCCTGCTTGATGGCGCGGCGCTGCCGGTGGCTATCGAACCTTTACAGGTCATTGTTATTGCGCTGGTGGCGATGGCTATCGCGCTGCTGTCTACGCTTTACCCTTCATGGCGCGCTGCCGCCACTCAACCCGCTGAGGCTTTACGTTATGAATAAGATCCTGTTGCAATGCGACAACCTGTGCAAACGCTATCAGGAAGGCAGTGTGCAAACCGATGTGCTGCACAATGTCAGTTTCAGCGTCGGCGAAGGTGAAATGATGGCGATCGTCGGTAGCTCTGGTTCCGGTAAAAGTACCTTGCTGCACCTGCTGGGCGGGCTGGATACGCCAACCTCCGGCGATGTGATTTTTAATGGTCAGCCAATGAGCAAACTG  >  NC_000913/1176356‑1176846                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                     catgtgggTCTTGATGGTGCAAGGGGCCAGCGCCGGGATTATCGGTGCGATCCTCGGAGCGGCGCTTGGCGCCCTGCTTGCCGGCCAGTTAAATAATCTGAGGCCGAGAACCGGCGTCCGGGTTGATGGCGCGGCGCTGCCGGTGGCGATCGACCCTTTACAGGTCATTGTTGTTGCGCGGGTGGCGATGGCTATCGCGCTGCTTTCTCCGCTTTACCCTTCATGGCGCGCTGCCGCCACTCAACctgctg                                                                                                                                                                                                                                                  <  2:444347/245‑1 (MQ=255)   tgatgGTCTTTATGGTGCAAGGGGCCAGCGCCGGGATTATCGGTGCGATCCTCGGAGCGGCGCTTGGCGCCCTGCTTGCCAGCCAGTTAAATAATCTGATGCCGATAATCGGCGTCCTGCTTGATGGCGCGGCGCTGCCGGTGGCTATCGAACCTTTACAGGTCATTGTTATTGCGCTGGTGGCGATGGCTATCGCGCTGCTGTCTACGCTTTACCCTTCATGGCGCGCTGCCGCCACTCAACctgctg                                                                                                                                                                                                                                                  >  1:331210/1‑249 (MQ=255)       ggTCTTTATGGTGCAAGGGGCCAGCGCCGGGATTATCGGTGCGATCCTCGGAGCGGCGCTTGGCGCCCTGCTTGCCAGCCAGTTAAATAATCTGATGCCGATAATCGGCGTCCTGCTTGATGGCGCGGCGCTGCCGGTGGCTATCGAACCTTTACAGGTCATTGTTATTGCGCTGGTGGCGATGGCTATCGCGCTGCTGTCTACGCTTTACCCTTCATGGCGCGCTGCCGCCACTCAACCTGCTGAGGCt                                                                                                                                                                                                                                             <  1:39156/250‑1 (MQ=255)              aTGGTGCAAGGGGCCAGCGCCGGGATTATCGGTGCGATCCTCGGAGCGGCGCTTGGCGCCCTGCTTGCCAGCCAGTTAAATAATCTGATGCCGATAATCGGCGTCCTGCTTGATGGCGCGGCGCTGCCGGTGGCTATCGAACCTTTACAGGTCATTGTTATTGCGCTGGTGGCGGTGGCTATCGCGCTGCTGTCTACGCTTTACCCTTCATGGCGCGCTGCCGCCACTCAACCTGCTGAGGCTTTACGtt                                                                                                                                                                                                                                      >  1:121747/1‑250 (MQ=255)               tGGTGCAAGGGCCCAGCGCCGGGATTATCGGTGCGATCCTCGGAGCGGCGCTTGGCGCCATGCTGGCCAGCCAGTTAAATAATCTGATGACGATAATCGGCGTCCTGGTTGATGGCGCGGCGATGCCGGAGGCTATCGAACCTTTACAGGACATTGTTATTGCGCTGGTGGCGATGGCTATCGAGCTGCTGTCTACGATTTACCCTTCCTGGCGAGCTGCCGCCACTCAACCTGATGAGGCTTTACGTTAt                                                                                                                                                                                                                                    >  2:311064/1‑251 (MQ=255)                    cAAGGGGCCAGCGCCGGGATTATCGGTGCGATCCTCGGAGCGGCGCTTGGCGCCCTGCTTGCCAGCCAGTTAAATAATCTGATGCCGATAATCGGCGTCCTGCTTGATGGCGCGGCGCTGCCGGTGGCTATCGAACCTTTACAGGTCATTGTTATTGCGCTGGTGGCGATGGCTATCGCGCTGCTGTCTACGCTTTACCCTTCATGGCGCGCTGCCGCCACTCAACCTGCTGAGGCTTTACGTTATGAAt                                                                                                                                                                                                                                >  1:281353/1‑250 (MQ=255)                     aaGGGGCCAGCGCCGGGATTATCGGTGCGATCCTCGGAGCGGCGCTTGGCGCCCTGCTTGCCAGCCAGTTAAATAATCTGATGCCGATAATCGGCGTCCTGCTTGATGGCGCGGCGCTGCCGGTGGCTATCGAACCTTTACAGGTCATTGTTATTGCGCTGGTGGCGATGGCTATCGCGCTGCTGTCTACGCTTTACCCTTCATGGCGCGCTGCCGCCACTCAACCTGCTGAGGCTTTACGTTATGAATa                                                                                                                                                                                                                               >  1:113864/1‑250 (MQ=255)                              cgaccGGGATGATCGGGGCGACCCCCGGAGCGGCGCTGGGCGCCCTGCTTGCCAGCCAGTTAAATAATCTGATGCCTATAATCGGCGTCCTGCTTGATGGCGCGGCGCTGCCGGTGGCTATCGAACCTTTACAGGTCAGTGTTATTGCGCTGGTGGCGATGGCTATCGCGCTGCTGTCTACGCTTTACCCTTCATGGCGCGCTGCCGCCACTCAACCTGCTGAGGCTTTACGTTATGAATAAGATCCTGtt                                                                                                                                                                                                                     <  2:84901/248‑1 (MQ=255)                                    ggATTATCGGTGCGATCCTCGGAGCGGCGCTTGGCGCCCTGCTTGCCAGCCAGTTAAATAATCTGATGCCGATAATCGGCGTCCTGCTTGATGGCGCGGCGCTGCCGGTGGCTATCGAACCTTTACAGGTCATTGTTATTGCGCTGGTGGCGATGGCTATCGCGCTGCTGTCTACGCTTTACCCTTCATGGCGCGCTGCCGCCACTCAACCTGCTGAGGCTTTACGTTATGAATAAGATCCTGtt                                                                                                                                                                                                                     >  1:84901/1‑245 (MQ=255)                                       ttATCGGTGCTATCCTTGGAGCGGCGCTTGGCGCCCTGATTGCCAGCCAGTTAAATAATCTGATGCCGATAATCTGCGTCCTGCTTGATGGCGCGGCCATGCCGGTGGCTATCGAACCTTTACAGGGCATTTTTATTGCGCTGGTGGCGATGGCTATCGCGCTGCTGTCTACGCTTTGCCCTTCATGGCGCGCTGCCGCCACTCAACCTGCTGAGGCGTTACGTTATGAATAAGATCCTGTTGCAATGCg                                                                                                                                                                                                             <  2:312471/250‑1 (MQ=255)                                         aTCGGTGCGATCCTCGGAGCGGCGCTTGGCGCCCTGCTTGCCAGCCAGTTAAATAATCTGATGCCGATAAGCGGCGTCCTGCTTGATGGCGCGGCGCTGCCGGTGGCTATCGAACCTTTACAGGTCATTGTTATTGCGCTGGTGGCGATGGCTATCGCGCTGCTGTCTACGCTTTACCCTTCATGGCGCGCTGCCGCCACTCAACCTGCTGAGGCTTTACGTTATGAATAAGATCCTGTTGCAATGCGAc                                                                                                                                                                                                           <  1:391610/250‑1 (MQ=255)                                             gTGCGATCCGCGGAGCGGCGCTTGGAGCCCTGCTTGCCAGCCAGTTAAATAATCTGATGCGGATAATCGGCGTCCTGCTGGATGGCGCGGCGCTGCGGGTGGCTATCGAACCTTTACAGGTCATTGTTATTGCGCTGGTGGCGATGGCTATCGCGTTGCTGTCTACGCTTTACCCTGCATGGCGCGCTGCCTCCACTCAACCTGCTGAGGCTTTACGTTATGAATAAGATCCTGTTGCAATGCGACAACCt                                                                                                                                                                                                      <  1:306800/251‑1 (MQ=255)                                              ggCGATCCTCGGAGCGGCGCTTGGCGCCCTGCTTGCCAGCCAGTTAAATAATCTGAGGCCGATAATCGGCGTCCTGCTTGATGGCGCGGCGCTGCCGGTGGCTATCGAACCTTTACAGGTCATTGTTATTGCGCGGGTGGCGATGGCTATCGCGCTGCTGTCTACGCTTTACCCTTCATGGCGCGCTGCCGCCACTCAACCTGCTGAGGCTTTACGTTATGAATAAGATCCTGTTGCAATGCGACAACCtg                                                                                                                                                                                                     <  1:28065/250‑1 (MQ=255)                                                cGATCCTCGGAGCGGCGCTTGGCGCCCTGCTTGCCAGCCAGTTAAATAATCTGATGCCGATAATCGGCGTCCTGCTTGATGGCGCGGCGCTGCCGGTGGCTATCGAACCTTTACAGGTCATTGTTATTGCGCTGGTGGCGATGGCTATCGCGCTGCTGTCTACGCTTTACCCTTCATGGCGCGCTGCCGCCACTCAACCTGCTGAGGCTTTACGTTATGAATAAGATCCTGTTGCAATGCGACAACCtgtg                                                                                                                                                                                                   >  1:99065/1‑251 (MQ=255)                                                        ggAGCGGCGCTTGGCGCCCTGCTTGCCAGCCAGTTAAATAATCTGATGCCGATAATCGGCGTCCTGCTTGATGGCGCGGCGCTGCCGGTGGCTATCGAACCTTTACAGGTCATTGTTATTGCGCTGGTGGCGATGGCTATCGCGCTGCTGTCTACGCTTTACCCTTCATGGCGCGCTGCCGCCACTCAACCTGCTGAGGCTTTACGTTATGAATAAGATCCTGTTGCAATGCGACACCCTGTGCAAACGCt                                                                                                                                                                                           >  1:247972/1‑251 (MQ=255)                                                            cggcgCTTGGCGCCCTGCTTGCCAGCCAGTTAAATAATCTGATGCCGATAATCGGCGTCCTGCTTGATGGCGCGGCGCTGCCGGTGGCTATCGAACCTTTACAGGTCATTGTTATTGCGCTGGTGGCGATGGCTATCGCGCTGCTGTCTACGCTTTACCCTTCATGGCGCGCTGCCGCCACTCAACCTGCTGAGGCTTTACGTTATGAATAAGATCCTGTTGCAATGCGACAACCTGTGCAAACGCTATc                                                                                                                                                                                        >  2:242223/1‑250 (MQ=255)                                                                               tGCCAGCCAGTTAAATAATCTGATGCCGATAATCGGCGTCCTGCTTGATGGCGCGGCGCTGCCGGTGGCTATCGAACCTTTACAGGTCATTGTTATTGCGCTGGTGGCGATGGCTATCGCGCTGCTGTCTACGCTTTACCCTTCATGGCGCGCTGCCGCCACTCAACCTGCTGAGGCTTTACGTTATGAATAAGATCCTGTTGCAATGCGACAACCTGTGCAAACGCTATCAGGAAGGCAGTGTGCaaa                                                                                                                                                                      <  1:235288/249‑1 (MQ=255)                                                                               tGCCAGCCAGTTAAATAATCTGATGCCGATAATCGGCGTCCTGCTTGATGGCGCGGCGCTGCCGGTGGCTATCGAACCTTTACAGGTCATTGTTATTGCGCTGGTGGCGATGGCTATCGCGCTGCTGTCTACGCTTTACCCTTCATGGCGCGCTGCCGCCACTCAACCTGCTGAGGCTTTACGTTATGAATAAGATCCTGTTGCAATGCGACAACCTGTGCAAACGCTATCAGGAAGGCAGTGTGCAAAcc                                                                                                                                                                    >  2:79360/1‑251 (MQ=255)                                                                                gccagccaGTTAAATAATCTGATGCCGATAATCGGCGTCCTGCTTGATGGCGCGGCGCTGCCGGTGGCTATCGAACCTTTACAGGTCATTGTTATTGCGCTGGTGGCGATGGCTATCGCGCTGCTGTCTACGCTTTACCCTTCATGGCGCGCTGCCGCCACTCAACCTGCTGAGGCTTTACGTTATGAATAAGATCCTGTTGCAATGCGACAACCTGTGCAAACGCTATCAGGAAGGCAGTGTGCAAACCg                                                                                                                                                                   >  1:105990/1‑251 (MQ=255)                                                                                   agccagTTAAATAATCTGATGCCGATAATCGGCGTCCTGCTTGATGGCGCGGCGCTGCCGGTGGCTATCGAACCTTTACAGGTCATTGTTATTGCGCTGGTGGCGATGGCTATCGCGCTGCTGTCTACGCTTTACCCTTCATGGCGCGCTGCCGCCACTCAACCTGCTGAGGCTTTACGTTATGAATAAGATCCTGTTGCAATGCGACACCCTGTGCAAACGCTATCAGGAAGGCAGTGTGCAAACCGAtg                                                                                                                                                                >  2:216525/1‑251 (MQ=255)                                                                                                        ccGATAATCGGCGTCCTGCTTGATGGCGCGGCGCTGCCGGTGGCTATCGAACCTTTACAGGTCATTGTTATTGCGCTGGTGGCGATGGCTATCGCGCTGCTGTCTACGCTTTACCCTTCATGGCGCGCTGCCGCCACTCAACCTGCTGAGGCTTTACGTTATGAATAAGATCCTGTTGCAATGCGACAACCTGTGCAAACg                                                                                                                                                                                             >  2:346017/1‑201 (MQ=255)                                                                                                        ccGATAATCGGCGTCCTGCTTGATGGCGCGGCGCTGCCGGTGGCTATCGAACCTTTACAGGTCATTGTTATTGCGCTGGTGGCGATGGCTATCGCGCTGCTGTCTACGCTTTACCCTTCATGGCGCGCTGCCGCCACTCAACCTGCTGAGGCTTTACGTTATGAATAAGATCCTGTTGCAATGCGACAACCTGTGCAAACg                                                                                                                                                                                             <  1:346017/201‑1 (MQ=255)                                                                                                           aTAATCGGCGTCCTGCTTGATGGCGCGGCGCTGCCGGTGGCTATCGAACCTTTACAGGTCATTGTTATTGCGCTGGTGGCGATGGCTATCGCGCTGCTGTCTACGCTTTACCCTTCATGGCGCGCTGCCGCCACTCAACCTGCTGAGGCTTTACGTTATGAATAAGATCCTGTTGCAATGCGACAACCTGTGCAAACGCTATCAGGAAGGCAGTGTGCAAACCGATGTGCTGCACAATGTCAGTTTCAGCg                                                                                                                                        <  1:216525/251‑1 (MQ=255)                                                                                                              accggcgtccttctgtagggcgcggcgcTGCCGGTGGATATCTAACCTTTACAGGCCATTTGTATTGCTCTGGTGGTGATGGCTATCGCGCTTCTGTCTACGCTTTACCCTTCAGGGCGCGCTGCCGCCACTCAACCTGCTGAGGCTTTACGTTATGAATAAGATCCTGTTGCAATGCGACAACCTGTGCAAACGCTATCAGGAAGGCAGTGTGCAAACCGATGTGCTGCACAATGTCAGTTTCAGCGTCg                                                                                                                                     <  2:121747/233‑1 (MQ=255)                                                                                                                 ggCGTCCTGCTTGATGGCGCGGCGCTGCCGGTGGCTATCGAACCTTTACAGGTCATTGTTATTGCGCTGGTGGCGATGGCTATCGCGCTGCTGTCTACGCTTTACCCTTCATGGCGCGCTGCCGCCACTCAACCTGCTGAGGCTTTACGTTATGAATAAGATCCTGTTGCAATGCGACAACCTGTGCAAACGCTATCAGGAAGGCAGTGTGCAAACCGATGTGCTGCACAATGTCAGTTTCAGCGTCGGCg                                                                                                                                  >  1:365412/1‑251 (MQ=255)                                                                                                                                ggcgcggcgcTGCCGGTGGCTATCGAACCTTTACAGGTCATTGTTATTGCGCTGGTGGCGATGGCTATCGCGCTGCTGTCTACGCTTTACCCTTCATGGCGCGCTGCCGCCACTCAACCTGCTGAGGCTTTACGTTATGAATAAGATCCTGTTGCAATGCGACAACCTGTGCAAACGCTATCAGGAAGGCAGTGTGCAAACCGATGTGCTGCACAATGTCAGTTTCAGCGTCGGCGAAGGTGAAATGATgg                                                                                                                   >  1:319992/1‑251 (MQ=255)                                                                                                                                   gcggcgcTGCCGGTGGCTATCGAACCTTTACAGGTCATTGTTATTGCGCGGGTGGCGATGGCGATCGCGCTGCTGTCTACGCTTTACCCTTCATGGCGCGCTGCCGCCACTCAACCTGCTGAGGCTTTACGTTATGAATAAGATCCTGTTGCAATGCGACAACCTGTGCAAACGCTATCAGGAAGGCAGTGTGCAAACCGATGTGCTGCACAATGTCAGTTTCAGCGTCGGCGAAGGTGAAATGATGGCGa                                                                                                                <  2:105990/251‑1 (MQ=255)                                                                                                                                     ggcgcTGCCGGTGGCTATCGACCCTTTACAGGTCATTGTTATTGCGCTGGTGGCGATGGCTATCGCGCTGCTGTCTACGCTTTACCCTTCATGGCGCGCTGCCGCCACTCAACCTGCTGAGGCTTTACGTTATGAATAAGATCCTGTTGCAATGCGACAACCTGTGCAAACGCTATCAGGAAGGCAGTGTGCAAACCGATGTGCTGCACAATGTCAGTTTCAGCGTCGGCGAAGGTGAAATGATGGCGAtc                                                                                                              <  1:216061/251‑1 (MQ=255)                                                                                                                                                     aTCGAACCTTTACAGGTCATTGTTATGGCGCTGGTGGCGAGGGCTATCGCGCTGCTGTCTACGCTTTACCCTTCATGGCGCGCTGCCGCCACTCAACCTGCTGAGGCTTTACGTTATGAATAAGATCCTGTTGCAATGCGACAACCTGTGCAAACGCTATCAGGAAGGCAGTGTGCAAACCGATGTGCTGCACAATGTCAGTTTCAGCGTCGGCGAAGGTGAAATGATGGCGATCGTCGGTAGCTCTGGtt                                                                                              <  2:319992/251‑1 (MQ=255)                                                                                                                                                        gagccTTTACGGGTCATTGTTTTTGCGCTGGTGGCGATGGCTATCGCGCTGTAGTCTACGCTTTTCCCTTCATGGCGCGCTGCCGCCACTCAACCTGCTGAGGCTTTACGTTATGAATAAGATCCTGTTGCAATGCGACAACCTGTGCAAACGCTATCAGGAAGGCAGTGTGCAAACCGATGTGCTGCACAATGTCAGTTTCTGCGTCGGCGAAGGTGAAATGATGGCGATCGTCGGTAGCTCTGGTTCCg                                                                                           <  2:244237/248‑1 (MQ=255)                                                                                                                                                            cTTTACAGGTCATTGTTATTGCGCTGGTGGCGATGGCTATCGCGCTGCTGTCTACGCTTTACCCTTCATGGCGCGCTGCCGCCACTCAACCTGCTGAGGCTTTACGTTATGAATAAGATCCTGTTGCAATGCGACAACCTGTGCAAACGCTATCAGGAAGGCAGTGTGCAAACCGATGTGCTGCACAATGTCAGTTTCAGCGTCGGCGAAGGTGAAATGATGGCGATCGTCGGTAGCTCTGGTTCCgg                                                                                          <  1:273358/248‑1 (MQ=255)                                                                                                                                                            cTTTACAGGTCATTGTTATTGCGCTGGTGGCGATGGCTATCGCGCTGCTGTCTACGCTTTACCCTTCATGGCGCGCTGCCGCCACTCAACCTGCTGAGGCTTTACGTTATGAATAAGATCCTGTTGCAATGCGACAACCTGTGCAAACGCTATCAGGAAGGCAGTGTGCAAACCGATGTGCTGCACAATGTCAGTTTCAGCGTCGGCGAAGGTGAAATGATGGCGATCGTCGGTAGCTCTGGTTCCgg                                                                                          >  2:273358/1‑248 (MQ=255)                                                                                                                                                                 cAGGTCATGGTTATTGCGCTGGGGGCGATGGCGATCGTGCTGCTGTCTACGCTTTCCCCTTCATGGCGCGCTGCCGCCACTCAACCTGCTGAGGCTTTACGTTATTAATAAGATCCTGTTGCAATGCGACATCCTGTGCAAACGCTATCAGGAAGGCAGTGTGCAAACCGAGGTGCTGCACAATGTCAGTTTCAGCGTCGGCGAAGGTGAAATGATGGCGATCGTCGGTAGCTCTGGTTCCGGTAAAAGTa                                                                                  <  1:21010/251‑1 (MQ=255)                                                                                                                                                                               tGCGCTGGTGGCGATGGCTATCGCGCTGCTGTCTACGCTTTACCCTTCATGGCGCGCTGCCGCCACTCAACCTGCTGAGGCTTTACGTTATGAATAAGATCCTGTTGCAATGCGACAACCTGTGCAAACGCTATCAGGAAGGCAGTGTGCAAACCGATGTGCTGCACAATGTCAGTTTCAGCGTCGGCGAAGGTGAAATGATGGCGATCGTCGGTAGCTCTGGTTCCGGTAAAAGTACCTTGCTGCCCctg                                                                    >  2:347035/1‑251 (MQ=255)                                                                                                                                                                                 cgcTGGTGGCGATGGCTATCGCGCTGCTGTCTACGCTTTACCCTTCATGGCGCGCTGCCGCCACTCAACCTGCTGAGGCTTTACGTTATGAATAAGATCCTGTTGCAATGCGACAACCTGTGCAAACGCTATCAGGAAGGCAGTGTGCAAACCGATGTGCTGCACAATGTCAGTTTCAGCGTCGGCGAAGGTGAAATGATGGCGATCGTCGGTAGCTCTGGTTCCGGTAAAAGTACCTTGCTGCACctgct                                                                  <  1:422195/251‑1 (MQ=255)                                                                                                                                                                                     ggtggCGATGGCTATCGCGCTGCTGTCTACGCTTTACCCTTCATGGCGCGCTGCCGCCACTCAACCTGCTGAGGCTTTACGTTATGAATAAGATCCTGTTGCAATGCGACAACCTGTGCAAACGCTATCAGGAAGGCAGTGTGCAAACCGATGTGCTGCACAATGTCAGTTTCAGCGTCGGCGAAGGTGAAATGATGGCGATCGTCGGTAGCTCTGGTTCCGGTAAAAGTACCTtgctg                                                                          >  1:256854/1‑239 (MQ=255)                                                                                                                                                                                     gggggCGATGGCTATCGCGCTGCTGTCTACGCTTTACCCTTCAGGGCGCGCTGCCGCCACTCAACCTGCTGAGGCTTTACGTTATGAATAAGATCCTGTTGCAATGCGACAACCTGTGCAAACGCTATCAGGAAGGCAGTGTGCAAACCGATGTGCTGCACAATGTCAGTTTCAGCGTCGGCGAAGGTGAAATGATGGCGATCGTCGGTAGCTCTGGTTCCGGTAAAAGTACCTtgctg                                                                          <  2:256854/236‑1 (MQ=255)                                                                                                                                                                                          cGATGGCTATCGCGCTGCTGTCTACGCTTTACCCTTCATGGCGCGCTGCCGCCACTCACCCTGCTGAGGCTTTACGTTATGAATAAGATCCTGTTGCAATGCGACAACCTGTGCAAACGCTATCAGGAAGGCAGTGTGCAAACCGATGTGCTGCACAATGTCAGTTTCAGCGTCGGCGAAGGTGAACTGATGGCGATCGTCGGTAGCTCTGGTTCCGGTAAAAGTACCTTTCTGCCCCTTCTGGGCGGGCt                                                         >  2:285327/1‑251 (MQ=255)                                                                                                                                                                                                  aTCGCGCTGCTGTCTACGCTTTACCCTTCATGGCGCGCTGCCGCCACTCAACCTGCTGAGGCTTTACGTTATGAATAAGATCCTGTTGCAATGCGACAACCTGTGCAAACGCTATCAGGAAGGCAGTGTGCAAACCGATGTGCTGCACAATGTCAGTTTCAGCGTCGGCGAAGGTGAAATGATGGCGATCGTCGGTAGCTCTGGTTCCGGTAAAAGTACCTTGCTGCACCTGCTGGGCGGGCTGGATACg                                                  <  1:79360/250‑1 (MQ=255)                                                                                                                                                                                                    cgcgCTGCTGTCTACGCTTTACCCTTCATGGCGCGCTGCCGCCACTCAACCTGCTGAGGCTTTACGTTATGAATAAGATCCTGTTGCAATGCGACAACCTGTGCAAACGCTATCAGGAAGGCAGTGTGCAAACCGATGTGCTGCACAATGTCAGTTTCAGCGTCGGCGAAGGTGAAATGATGGCGATCGTCGGTAGCTCTGGTTCCGGTAAAAGTACCTTGCTGCACCTGCTGGGCGGGCTGGATACGcc                                                >  1:175546/1‑250 (MQ=255)                                                                                                                                                                                                       gctgctGTCTACGCTTTACCCTTCATGGCGCGCTGCCGCCACTCAACCTGCTGAGGCTTTACGTTATGACTAAGATCCTGTTGCAATGCGACACCCTGTGCAAACGATATCAGGAAGGCAGTGTGCAAACCGATGTGCTGCACAATGTCAGTTTCAGCGTCGGCGAAGGAGCAATGATGGCGATCGTCGGTAGCTCTGGTTCCGGTAACAGTACCTTGCTGCACCTGCTGGGCGGGCTGGCTACGCCCAcc                                            >  1:303385/1‑251 (MQ=255)                                                                                                                                                                                                          gctgTCTACGCTTTACCCTTCATGGCGCGCTGCCGCCACTCAACCTGCTGAGGCTTTACGTTATGAATAAGATCCTGTTGCAATGCGACAACCTGTGCAAACGCTATCAGGAAGGCAGTGTGCAAACCGATGTGCTGCACAATGTCAGTTTCAGCGTCGGCGAAGGTGAAATGATGGCGATCGTCGGTAGCTCTGGTTCCGGTAAAAGTACCTTGCTGCACCTGCTGGGCGGGCTGGATACGCGAACCTcc                                         >  2:156882/1‑251 (MQ=255)                                                                                                                                                                                                             gTCTACGCTTTACCCTTCATGGCGCGCTGCCGCCACTCAACCTGCTGAGGCTTTACGTTATGAATAAGATCCTGTTGCAATGCGACAACCTGTGCAAACGCTATCAGGAAGGCAGTGTGCAAACCGATGTGCTGCACAATGTCAGTTTCAGCGTCGGCGAAGGTGAAATGATGGCGATCGTCGGTAGCTCTGGTTCCGGTAAAAGTACCTTGCTGCACCTGCTGGGCGGGCTGGATACGCCAACCTCCGGc                                      >  1:301997/1‑251 (MQ=255)                                                                                                                                                                                                                 ccGCTTTACCCTTCATGGCGCGCTCCCGCCACTCAACCTGCTGAGGCTTTACGTTATGAATAAGATCCTGTTGCAATGCGACAACCTGTGCAAACGCTATCAGGAAGGCAGTGTGCAAACCGATGTGCTGCACAATGTCAGTTTCAGCGTCGGCGAAGGTGAAATGATGGCGATCGTCGGTAGCTCTGGTTCCGGTAAAAGTACCTTGCTGCACCTGCTGGGCGGGCTGGATACGCCAACCTCCGGCGAtg                                  <  2:113864/250‑1 (MQ=255)                                                                                                                                                                                                                  cGCTTTACCCTTCATGGCGCGCTGCCGCCACTCAACCTGCTGAGGCTTTACGTTATGAATAAGATCCTGTTGCAATGCGACAACCTGTGCAAACGCTATCAGGAAGGCAGTGTGCAAACCGATGTGCTGCACAATGTCAGTTTCAGCGTCGGCGAAGGTGAAATGATGGCGATCGTCGGTAGCTCTGGTTCCGGTAAAAGTACCTTGCTGCACCTGCTGGGCGGGCTGGCTACTCCACCCTCCGGCGAtgt                                 >  2:249637/1‑251 (MQ=255)                                                                                                                                                                                                                    cTTTACCCTTCATGGCGCGCTGCCGCCACTCAACCTGCTGAGGCTTTACGTTATGAATAAGATCCTGTTGCAATGCGACAACCTGTGCAAACGCTATCAGGAAGGCAGTGTGCAAACCGATGTGCTGCACAATGTCAGTTTCAGCGTCGGCGAAGGTGAAATGATGGCGATCGTCGGTAGCTCTGGTTCCGGTAAAAGTACCTTGCTGCACCTGCTGGGCGGGCTGGATACGCCAACCTCCGGCGAtgtg                                >  2:172874/1‑250 (MQ=255)                                                                                                                                                                                                                       tACCCTTCATGGCGCGCTGCCGCCACTCAACCTGCTGAGGCTTTACGTTATGAATAAGATCCTGTTGCAATGCGACAACCTGTGCAAACGCTATCAGGAAGGCAGTGTGCAAACCGATGTGCTGCACAATGTCAGTTTCAGCGTCGGCGAAGGTGAAATGATGGCGATCGTCGGTAGCTCTGGTTCCGGTAAAAGTACCTTGCTGCACCTGCTGGGCGGGCTGGATACGCCAACCTCCGGCGATGTGAttt                            >  1:149806/1‑251 (MQ=255)                                                                                                                                                                                                                          ccTTCATGGCGCGCTGCCGCCACTCAACCTGCTGAGGCTTTACGTTATGAATAAGATCCTGTTGCAATGCGACAACCTGTGCATACGCTATCAGGCAGGCAGTGTGCAAACCGATGTGCTGCACAATGTCAGTTTCAGCGTCGGCGAAGGTGAAATGATGGCGATCGTCGGTAGCTCTGGTTCCGGTAAAAGTACCTTGATGCACCTGCTGGGCGGGCTGGATACGCCACCCTCCGGCGATGTGAttttt                          >  2:237188/1‑250 (MQ=255)                                                                                                                                                                                                                                         gccgccACTCAACCTGCTGAGGCTTTACGTTATGAATAAGATCCTGTTGCAATGCGACAACCTGTGCAAACGCTATCAGGAAGGCAGTGTGCAAACCGATGTGCTGCACAATGTCAGTTTCAGCGTCGGCGAAGGTGAAATGATGGCGATCGTCGGTAGCTCTGGTTCCGGTAAAAGTACCTTGCTGCACCTGCTGGGCGGGCTGGATACGCCAACCTCCGGCGATGTGATTTTTAATGGTCAGCCAATGa          <  1:242223/251‑1 (MQ=255)                                                                                                                                                                                                                                                 ccAACCTGCTGAGGCTTTACGTTATGAATAAGATCCTGTTGCAATGCGACAACCTGTGCAAACGCTATCAGGAAGGCAGTGTGCAAACCGATGTGCTGCACAATGTCAGTTTCAGCGTCGGCGAAGGTGAAATGATGGCGATCGTCGGTAGCTCTGGTTCCGGTAAAAGTACCTTGCTGCACCTGCTGGGCGGGCTGGATACGCCAACCTCCGGCGATGTGATTTTTAATGGTCAGCCAATGAGCAAACTg  <  1:181033/250‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                     CATGATGGTCTTTATGGTGCAAGGGGCCAGCGCCGGGATTATCGGTGCGATCCTCGGAGCGGCGCTTGGCGCCCTGCTTGCCAGCCAGTTAAATAATCTGATGCCGATAATCGGCGTCCTGCTTGATGGCGCGGCGCTGCCGGTGGCTATCGAACCTTTACAGGTCATTGTTATTGCGCTGGTGGCGATGGCTATCGCGCTGCTGTCTACGCTTTACCCTTCATGGCGCGCTGCCGCCACTCAACCCGCTGAGGCTTTACGTTATGAATAAGATCCTGTTGCAATGCGACAACCTGTGCAAACGCTATCAGGAAGGCAGTGTGCAAACCGATGTGCTGCACAATGTCAGTTTCAGCGTCGGCGAAGGTGAAATGATGGCGATCGTCGGTAGCTCTGGTTCCGGTAAAAGTACCTTGCTGCACCTGCTGGGCGGGCTGGATACGCCAACCTCCGGCGATGTGATTTTTAATGGTCAGCCAATGAGCAAACTG  >  NC_000913/1176356‑1176846

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑