Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,141,865	G→A	P835S (CCG→TCG)	rne ←	ribonuclease E

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,141,865	0	G	A	95.3%	250.5 / ‑2.4	87	P835S (CCG→TCG)	rne	ribonuclease E
Reads supporting (aligned to +/- strand):  ref base G (4/0);  new base A (38/44);  total (43/44)
Fisher's exact test for biased strand distribution p-value = 5.27e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCAGGATGCGTCGTTTCAACCACTTCCGGTTGCGGTTCGGCAACCTGAACGGGGGCTTCTACGACACCGGCCACTTCTTCAACAACTGGCGCGCTAACAACCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCTGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGA‑GGCCAGTTCCGGAGAC‑GCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCACGATAGCGACGACGACGCTGACCACTTACGCGCAGGTGACGAGGCGAGCGGCGAGAACGACGCGGCATGCCACCGTTGTCACGGTTATCAGCATTGTTCTCTTCTTGCTGTTCTGGTGCAGTTTGCGCTACGACTGGCAGCGGGACTTTCACCAGTTCTGTGCGTGGAGCTGGCGCTTCCTGAAC  >  NC_000913/1141621‑1142105                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                  tcgggagtcgtcgtTTCACCCCCTTCCGGTTTCGGCTCGGCAGCCTGTACGGGTGCTTCTACGCCTCCGGCCTCTTCTTCAACAACTGGCTCTCTAACAACCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTCTCCATCGGCTGCACATGGACTTCTTCCTGTTCGCGCTGCTCTTCAACCTGTACATCTTGCGGACGTACAATCGGATAGCGGATCCAGACTTTTCCAGA‑TGCCAGTTCCGAAGAc                                                                                                                                                                                                                                               <  2:399818/241‑1 (MQ=255) tcaggagtcgtcgtTTCCACCACTTCCGGTTGCAGTTCGGCAACCTGAACGGGGGCTTCTACGACAACGGCCACTTCTTCAACAACTGGCGCGCTAACAACCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCTGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGA‑GGCCAGTTCCGAAGAc                                                                                                                                                                                                                                               <  2:403526/241‑1 (MQ=255)     gactcgtcgtTGCACCCACTCCCGTTTGCGGTTCGGCAACCTGAACGGGGGCTCCTAAGACACCGGCCACTGCTTCACCAACTGGCGCGCTAACATCCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCGGCACATGTACTTCTTCCTGTTCGCGCTGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCTGATCCAGACTTTGCCAGA‑GGCCAGTTCCGAAGAC‑GCGCAc                                                                                                                                                                                                                                        <  2:451169/247‑1 (MQ=255)        gCGTCGTTTCAACCACTTCCGGTTGCGGTTCGGCAACCTGAACGGGGGCTTCTACGACACCGGCCACTTCTTCAACAACTGGCGCGCTAACAACCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCTGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGA‑GGCCAGTTCCGAAGAC‑gcgccagcc                                                                                                                                                                                                                                     >  1:445212/1‑246 (MQ=255)        gCGTCGTTTCAACCACTTCCGGTTGCGGTTCGGCAACCTGAACGGGGGCTTCTACGACACCGGCCACTTCTTCAACAACTGGCGCGCTAACAACCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCTGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGA‑GGCCAGTTCCGAAGAC‑GCGCACGCt                                                                                                                                                                                                                                     <  2:204194/251‑1 (MQ=255)          gtggtatcagcccggtCCGGTTGCGGGTAGGCAACCTGCAGTGGGGCTGCTACGACTCCGGCCACTCCTTCAACAACTGGCGCGCTAACAACCGGTTCGATAGCGGCGGCGTCAGGGACCTCAGTCTCCAGCGGCTGCACATGTACTGCTGCCTGTACGCGCTGGGCTTCAACCTGTTCATCTTGCGTGCGTACAAGTGGAGAGCGTATCGAGCCTTGGCCTGATGGCC‑GTTCCGAATAC‑GCTCACGCTAc                                                                                                                                                                                                                                   <  2:219179/236‑1 (MQ=255)           ccgtTTCAACCACTTCCGGTTGCGGTTCGGCAACCTGAACGGGGGCTTCTACGACACCGGCCACTTCTTCAACAACTGGCGCGCTAACAACCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCTGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGA‑GGCCAGTTCCGAAGAC‑GCGCACGCTACg                                                                                                                                                                                                                                  <  2:381699/250‑1 (MQ=255)                 cAACCACTTCCGGTTGCGGTTCGGCAACCTGAACGGGGGCTTCTACGACACCGGCCACTTCTTCAACAACTGGCGCGCTAACAACCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCTGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGA‑GGCCAGTTCCGAAGAC‑GCGCACGCTACGGTCAAc                                                                                                                                                                                                                            <  1:197432/251‑1 (MQ=255)                                  ggTTCGGCAACCTGAACGGGGGCTTCTACGACACCGGCCACTTCTTCAACAACTGGCGCGCTAACAACCGGTTCGATAGCGGCGGCGACAGGGCCCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCTGCTCTTCAAACTGTACATCTTGCGGCCGTACAATTGGATCGCGGATCCAGACTTTGCCAGA‑GGCATGTTCCG‑ACACCGCGCCCGCGACGGTCAACGGCGTTgggggccgggg                                                                                                                                                                                                           >  2:228616/1‑242 (MQ=255)                                  ggTTCGGCAACCTGAACGGGGGCTTCTACGACACCGGCCACTTCTTCAACAACTGGCGCGCTAACAACCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCTGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGA‑GGCCAGTTCCGAAGAA‑GCGCACGCTACGGTCAACGGCATTGGCGACTgggg                                                                                                                                                                                                           >  1:7792/1‑250 (MQ=255)                                          aaCCTGAACGGGGGCTTCTACGACACCGGCCACTTCTTCAACAACTGGCGCGCTAACAACCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCTGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGA‑GGCCAGTTCCGAAGAC‑GCGCACGCTACGGTCAACTGCATTGGCGACTGGGTTGGATAAc                                                                                                                                                                                                   <  1:407518/251‑1 (MQ=255)                                                  cGGGGGCTTCTACGACACCGGCCACTTCTTCAACAACTGGCGCGCTAACAACCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCTGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCCGA‑GGCCAGTTCCGAAGAC‑GCGCACGCTACGGTCAACGGCATTGGCGCCTGGGTTGGATAACGCTCGTc                                                                                                                                                                                            >  1:343144/1‑250 (MQ=255)                                                   gggggCTTCTCCGACACCGGCCACTTCTTCAACAACTGGCGCGCTAACAACCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCTGCTCTTCAACCTGTACATCTTGCGGACGTACAAGTGGATAGCGGATCCAGACTTTGCCAGA‑GGCCAGTTCCGAAGAC‑GCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCAc                                                                                                                                                                                          <  2:437452/251‑1 (MQ=255)                                                      ggCTTCTACGACACCGGCCACTTCTTCAACAACTGGCGCGCTAACAACCGGTTCGATAGCGGCGGCGACAGTGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCTGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGA‑GGCCAGTTCCGAAGAC‑GCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCACGAt                                                                                                                                                                                       <  1:36808/251‑1 (MQ=255)                                                       gCTTCTACGACACCGGCCACTTCTTCAACAACTGGCGCGCTAACAACCGGTTCGATAGCGGCGGCGACAGGGCCCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCTGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGA‑GGCCAGTTCCGAAGAC‑GCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCACGATa                                                                                                                                                                                      <  1:334469/251‑1 (MQ=255)                                                        cTTCTACGACACCGGCCACTTCTTCAACAACTGGCGCGCTAACAACCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCTGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGA‑GGCCAGTTCCGAAGAC‑GCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCACGAt                                                                                                                                                                                       >  1:419586/1‑249 (MQ=255)                                                          tCTACGACACCGGCCACTTCTTCAACAACTGGCGCGCTAACAACCGGTTCGATAGCGTCGGCGACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCTGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGA‑GGCCAGTTCCGAAGAC‑GCGCACGCTACGGTCAACGGCAATGGCGACTGGGTTGGATAACGCTCGTCACGCTAg                                                                                                                                                                                     <  2:92176/249‑1 (MQ=255)                                                          tCTACGACACCGGCCACTTCTTCAACAACTGGCGCGCTAACAACCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCTGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGA‑GGCCAGTTCCGAAGAC‑GCGCACGCTACGGTCAACGGCATTGGCGACAGGGTTGGATAACGCTCGTCACGATAg                                                                                                                                                                                     <  1:478318/249‑1 (MQ=255)                                                             aCGACACCGGCCACTTCTTCAACAACGGGCGCGCTAACAACCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCTGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGA‑GGCCAGTTCCGAAGAC‑GCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCACGATAGcgac                                                                                                                                                                                 <  1:379973/250‑1 (MQ=255)                                                               gACACCGGCCACTTCTTCAACAACTGGCGCGCTAACAACCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCTGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGA‑GGCCAGTTCCGAAGAC‑GCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGACAACGCTCGTCACGATAGcgacgac                                                                                                                                                                              >  1:124981/1‑251 (MQ=255)                                                                acacCGGCCACTTCTTCAACAACTGGCGCGCTAACAACCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCTGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGA‑GGCCAGTTCCGAAGAC‑GCGCACGCTACGGTCAACGGCATTGGCGCCTGGGTTGGATAACGCTCGTCACGATACcaacgacc                                                                                                                                                                             >  1:264028/1‑250 (MQ=255)                                                                acacCGGCCACTTCTTCAACAACTGGCGCGCTAACAACCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCTGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGA‑GGCCAGTTCCGAAGAC‑GCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCACGATAGcgacgacg                                                                                                                                                                             <  2:362286/251‑1 (MQ=255)                                                                 cacCGGCCACTTCTTCAACAACTGGCGCGCTAACAACCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCTGCTATTCAACCTGTACATCTTGCGGACGTACCATTGGCTAGCGGATCCAGACTTTGCCAGA‑GGACAGTTCCGACGAC‑GCGCACGCTACGGTCAACGGCCTTGGCGACTGGGTTGGCTAAAGCTCGTCACGATAGagacgacgg                                                                                                                                                                            >  1:246476/1‑250 (MQ=255)                                                                     ggCCACTTCTTCAACAACTGGCGCGCTAACAACCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCTGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGA‑GGCCAGTTCCGAAGAC‑GCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCACGATAGCGACGACGACGCt                                                                                                                                                                        >  1:60647/1‑251 (MQ=255)                                                                       ccACTTCTTCAACAACTGGCGCGCTAACAACCGGTTCGATAGCGGCGGCGACAGGGACCTCCGTCACCATCGGCTGCACATGTACTTCTTCCTGTTTGCGCTGCTCCTCAACCTGTACCTCTTGCGGACGTACCATTGGATAGCGGGTCCAGACTTTGCCAGA‑GGCCAGTTTCCAACAC‑GCGCACGCTACGGTCAACGGCACTGGCGACTGGGTTGGATAACGCTCGTCACGATATCGCCGCCGACGccgg                                                                                                                                                                      >  2:165378/1‑248 (MQ=255)                                                                         aCTTCTTCAACAACTGGCGCGCTAACAACCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCCGCACATGTACTTCTTCCTGTTCGCGCTGCTCTTCAACCTGTACATCTTTCGGACGTACAATTGGATAGCGGATCCCGACTTTGCCAGC‑GGCCAGTTCCGAAGAC‑GCGCCCGCTACGGTCAACGGCATTGGCGACTGGGGTGGATAACGCTCGTCACGCTAACGACGACGACGCTGaac                                                                                                                                                                    >  2:141470/1‑249 (MQ=255)                                                                                caacaaCTGGCGCGCTAACAACCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCTGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGA‑GGCCAGTTCCGAAGAC‑GCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCAAGATAGCGACGACGACGCTGACCACTTAcg                                                                                                                                                             >  1:52746/1‑251 (MQ=255)                                                                                 aacaacTGGCGCGCTAACAACCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCTGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGA‑GGCCAGTTCCGGAGAC‑GCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCACGATAGCGACGACGACGCTGACCACTTAcgc                                                                                                                                                            >  1:420833/1‑251 (MQ=255)                                                                                        ggCGCGCTAACAACCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCTGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGA‑GGCCAGTTCCGAAGAC‑GCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCACGATAGCGACGACGACGCTGACCACTTACGCGCAGGTg                                                                                                                                                     <  1:351665/251‑1 (MQ=255)                                                                                          cgcgcTAACAACCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCTGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGA‑GGCCAGTTCCGAAGAC‑GCGCACGCTACGGTCAACGGCATTGGCGACTGGGGTGGATAACGCTCGTCACGGTAGCGACGACGACGCTGACCACTTACGCGCAGGTGAc                                                                                                                                                   >  1:99054/1‑251 (MQ=255)                                                                                              cTAACAACCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCTGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGA‑GGCCAGTTCCGAAGAC‑GCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCACGATAGCGACGACGACGCTGACCACTTACGCGCAGGTGACGAgg                                                                                                                                               >  1:171248/1‑251 (MQ=255)                                                                                                aacaacCGGTTCGATAGCGGCGGCGACCGGGACCTCCGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCTGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGA‑GGCCAGTTCCGAAGAC‑GCGCCCGCTACGGTCCACGGCATTGGCGACTGGGTTGGATAACGCTCGTCCCGCACGCGACGCCGACGCTGACCCCTTACGCGCCGGGGGCGAGGCg                                                                                                                                             >  2:31198/1‑251 (MQ=255)                                                                                                 acaacCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTCACCATCTGCTGCACATGTACTTCTTCCTGTTCGCGCTGCTCTTCAACCTGTACATCTTGCGGACGTACCATTGGATAGCGGGTCCAGACTTTGCCAGA‑GGCCAGTTCCGGAGAC‑GCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGGTAACGCTCGTCCCGATAGCGACGGCGACGCTGACCCCTTACGCGCAGGTggacaggcgg                                                                                                                                            >  2:10515/1‑242 (MQ=255)                                                                                                               aGCGGCGGCGCCCGGGAACTCAGTCACCAGCGGCTGCACATGTACTTCTTCCTGTTCGCGCTGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGGTCCAGACTTTGCCAGA‑GGGCAGTTACGGAGAC‑GCGCACGCTACGGGCAACGGCATTGGCGACTGGGGTGGATAAAGCTCGGCCCGCTAACGACGACGACGCTGACCACTTCAGCGCAgggggcgcggggaggggggggaacggc                                                                                                                              >  2:254626/1‑226 (MQ=255)                                                                                                                 cggcggcgACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCTGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGA‑GGCCAGTTCCGAAGAC‑GCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCACGATAGCGACGACGACGCTGACCACTTACGCGCAGGTGACGAGGCGAGCGGCGAGAACGAcgc                                                                                                                            <  1:117897/251‑1 (MQ=255)                                                                                                                  gggtgcgccAGGGACCTCAGACACCAGCGGCTGCACATGTACTTCTTCCTGGTCGCGCGGCTCTTCAAACTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTGTGCCAGA‑GGCCAGTTCCGAAGAC‑GCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGAGAACGCTCGTCACGATAGAGACGACGACGCTGAACAATTACGCGCAGGTGACGAGGCGAGCGGCGAGAACGAcgcg                                                                                                                           <  2:261833/243‑1 (MQ=255)                                                                                                                     ggcgACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCTGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGA‑GGCCAGTTCCGAAGAC‑GCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCACGATAGCGACGACGACGCTGACCACTTACGCGCAGGTGACGAGGCGAg                                                                                                                                           >  1:258860/1‑232 (MQ=255)                                                                                                                     ggcgACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCTGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGA‑GGCCAGTTCCGAAGAC‑GCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCACGATAGCGACGACGACGCTGACCACTTACGCGCAGGTGACGAGGCGAg                                                                                                                                           <  2:258860/232‑1 (MQ=255)                                                                                                                       cgACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCTGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGA‑GGCCAGTTCCGAAGAC‑GCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCACGATAGCGACGACGACGCTGACCACTTACGCGCAGGTGACGAGGCGAGCGGCGAGAACGACGCGGCATg                                                                                                                      <  1:46893/251‑1 (MQ=255)                                                                                                                        gACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCTGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGA‑GGCCAGTTCCGAAGAC‑GCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCACGATAGCGACGACGACGCTGACCACTTACGCGCAGGTGACGAGGCGAGCGGCGAGAACGACACGGCATGc                                                                                                                     >  2:417344/1‑251 (MQ=255)                                                                                                                            gggACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCTGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGA‑GGCCAGTTCCGAAGAC‑GCGCACGCTACGGTCAACGGCATTGGCGACTGGGGTGGATAACGCTCGTCACGATAGCGACGACGACGCTGGCCACTTACGCGCAGGTGACGAGGCGAGCGGCGAGAACGACGCGGCCTGCCAcc                                                                                                                 >  2:158570/1‑251 (MQ=255)                                                                                                                             ggACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCTGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGA‑GGCCAGTTCCGAAGAC‑GCGCACGCTACGGTCCACGGCATTGGCGACTGGGTTGGATAACGCTCGTCCCGATAGCGACGACGACGCTGACCACTTACGCGCAGGGGGCGAGGCGAGGGGCGAGAACGACGCGGCATGCCACCg                                                                                                                >  2:289017/1‑251 (MQ=255)                                                                                                                               aCCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCTGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGA‑GGCCAGTTCCGAAGAC‑GCGCACGCTACGGTCACCGGCATTGGCGACTGGGTTGGATAACGCTCGTCACGATAGCGACGACGACGCTGACCACTTACGCGCAGTTGACCAGGCGAgcggcgggcaacgctcggcatgcacccggt                                                                                                              >  2:117460/1‑227 (MQ=255)                                                                                                                                   cAGTCAACATCGGCTGCACATTGACCTCTTCCTGTACGCTCTGCTCTTCGACCTGTACATCATGCGGACGTTCAATTGGATATCGGATCCAGACTGTGCCTGA‑GGCCAGTTCCGAAGTC‑GCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCACGATAGCGACGACGACGCTGACCACTTACGCGTAGGTGACGAGTCGAGCGGCGATATCGACGCGTCATGCCACCTTTGTCa                                                                                                          <  1:91952/251‑1 (MQ=255)                                                                                                                                    agtaaccaccggctggtaaTGTACGTCTTCAGGTTCGCGCTGATCGTCAACCTGTACATCTGGCGGACGTATAATGGGATAGTGGATCTAGACTTTTCCAGA‑GGCCAGTTCCGAAGAC‑GCGTACGCTACGGTCACCGGCATTGTCGACTGGGTTGGATAACGCGCGTCTCGATAGCTACGACGACGCGGACCACTTACGCGCAGGTGACGAGGCGAGCGGCCAGACCGACGCGTCACGCCACCGTTGTCAc                                                                                                         <  2:303125/233‑1 (MQ=255)                                                                                                                                     gTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCTGCTCTTCACCCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGA‑GGCCAGTTCCGAAGAC‑GCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCACGATAGCGACGACGACGCTGACCACTTACGCGCAGGTGACGAGGCGAGCGGCGGGAACGACGCGGCATGCCACCGTTGTCACg                                                                                                        >  1:278571/1‑251 (MQ=255)                                                                                                                                         ccATCGGCTGCACATGTACTTCTTCCTGTTCGCGCTGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGA‑GGCCAGTTCCGAAGAC‑GCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCACGATAGCGACGACGACGCTGACCACTGACGCGCAGGTGACGAGGCGAGCGGCGAGAACGACGCGGCATGCCACCGTTGTCACGGTTa                                                                                                    <  1:236676/251‑1 (MQ=255)                                                                                                                                           aTCGGCTGCACATGTACTTCTTCCTGTTCGCGCTGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGA‑GGCCAGTTCCGAAGAC‑GCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCACGATAGCGACGACGACGCTGACCACTTACGCGCAGGTGACGAGGCGAGCGGCGAGAACGACGCGGCATGCCACCGTTGTCACGGTTATc                                                                                                  >  1:483672/1‑251 (MQ=255)                                                                                                                                             cGGCTGCACATGTACTTCTTCCTGTTCGCGCTGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGA‑GGCCAGTTCCGAAGAC‑GCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCACGATAGCGACGACGACGCTGACCACTTACGCGCAGGTGACGGGGCGAGCGGCGAGAACGACGCAGCATGCCACCCTTGTCACGTTTATccg                                                                                                >  2:334967/1‑249 (MQ=255)                                                                                                                                             cGGCTGCACATGTACTTCTTCCTGTTCGCGCTGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGA‑GGCCAGTTCCGAAGAC‑GCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCACGATAGCGACGACGACGCTGACCACTTACGCGCAGGTGACGAGGCGAGCGGCGAGAACGACGCGGCATGCCACCGTTGTCACGGTTATCAg                                                                                                <  1:31198/251‑1 (MQ=255)                                                                                                                                              ggCTGCACATGTACTTCTTCCTGTTCGCGCTGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGA‑GGCCAGTTCCGAAGAC‑GCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCACGATAGCGACGACGACGCTGACCACTTACGCGCAGGTGACGAGGCGAGCGGCGAGAACGACGCGGCATGCCACCGTTGTCACGGTTATCAGc                                                                                               >  1:262777/1‑251 (MQ=255)                                                                                                                                                cTGCACATGTACTTCTTCCTGTTCGCGCTGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGA‑GGCCAGTTCCGAAGAC‑GCGCACGCTACGGTCAACGGCATTGGCGCCTGGGTTGGATAACGCTCGTCACGATAGCGACGACGACGCTGACCACTTACGCGCAGGTGACGAGGCGAGCGGCGAGAACGACGCGGCATGCCACCGTTGTCACGGTTATCAGCAt                                                                                             >  1:285298/1‑251 (MQ=255)                                                                                                                                                             tcttcCTGTTCGCGCTGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGA‑GGCCAGTTCCGAAGAC‑GCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCACGATAGCGACGACGACGCTGACCACTTACGCGCAGGTGACGAGGCGAGCGGCGAGAACGACGCGGCATGCCACCGTTGTCACGGTTATCAGCATTGTTCtcttct                                                                                  <  2:171248/249‑1 (MQ=255)                                                                                                                                                               ttcCTGTTCGCGCTGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGA‑GGCCAGTTCCGAAGAC‑GCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCACGATAGCGACGACGACGCTGACCACTTACGCGCAGGTGACGAGGCGAGCGGCGAGAACGACGCGGCATGCCACCGTTGTCACGGTTATCAGCATTGTTCTCTTCTTGCt                                                                              >  1:335840/1‑251 (MQ=255)                                                                                                                                                               ttcCTGTTCGCGCTGATCTTCAACCTGCACATCTTGCGGACGTACAATTGGATAGCGGATCCAGAATTTGCCAGC‑GGCCAGTTCCGAAGAC‑GCGCACGCAACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCACGATAGCGACGCCGACGCTGACCACTTACGCGCAGGAGACGAGGCGAGCGGCGAGAACGACGCGGCCTGCCACCGTTGTCACGGTTATCAGCGTGGTTCACTTCTTGCt                                                                              >  2:96144/1‑251 (MQ=255)                                                                                                                                                                   tGTTCGCGCTGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGA‑GGCCAGTTCCGAAGAC‑GCGCACGCTACGGTCAACGGCATTGGCGCCTGGGTTGGATAACGCTCGTCACGATAGCGACGACGACGCTGACCACTTACGCGCAGGTGACGAGGCGAGCGGCgaga                                                                                                                                   >  2:192236/1‑194 (MQ=255)                                                                                                                                                                   tGTTCGCGCTGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGA‑GGCCAGTTCCGAAGAC‑GCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCACGATAGCGACGACGACGCTGACCACTTACGCGCAGGTGACGAGGCGAGCGGCgaga                                                                                                                                   <  1:192236/194‑1 (MQ=255)                                                                                                                                                                    gTTCGCGCTGCTCTTCATCCTGTTCATCTTGCGGACGTACAATTGGATAGCGGATTCAGCCTTGTCTAGA‑GGCCAGTTCCGAAGAC‑GCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGTATAACTCTCGTCACGATAGCGACGACGACGCTGACCACGTACGCGAAGGTGACGAGGCGATCGGCGAGATCGACGCGGCATGCCACCGTTGTCACGGTTAGCCGCATTGTTCTCTTCTTGCTGtt                                                                           <  2:190362/249‑1 (MQ=255)                                                                                                                                                                       cgcgCTGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGA‑GGCCAGTTCCGAAGAC‑GCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCGCGTCACGATAGCGACGACGACGCTGACCACTTACGCGCAGGTGACGAGGCTAGCGGCGAGAACGACGCGGCATGCCACCGTTGTCACGGTTATCAGCATTGTTCTCTTCTTGCTGTTCTgg                                                                       <  2:299099/250‑1 (MQ=255)                                                                                                                                                                        gcgcTGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGA‑GGCCAGTTCCGAAGAC‑GCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCACGATAGCGACGACGACGCTGACCACTTACGCGCAGGTGACGAGGCGAGCGGCGAGAACGACGCGGCATGCCACCGTTGTCACGGTTATCAGCATTGTTCTCTTCTTGCTGTTCTGGTg                                                                     <  1:48419/251‑1 (MQ=255)                                                                                                                                                                          gctgctCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGA‑GGCCAGTTCCGAAGAC‑GCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCACGATAGCGACGACGCCGCTGACCACTTACGCGCAGGTGACGAGGCGAGCGGCGAGAACGACGCGGCATGCCACCGTTGTCACGGTTATCAGCATTGTTCTCTTCTTGCTGTTCTGGTGc                                                                    >  2:278164/1‑250 (MQ=255)                                                                                                                                                                             gctCTTCAACCTGTCCATCTTGCGGACGTACAATTGGATAGCGGATACAGACTTTGCCAGA‑GGCCAGTTCCGAAGAC‑GCGCACGCTACGGTCAACGGCATTGGCTACTGGGTTGGATAACGCTCGTCACGATAGCGACGACGACGCTGACCACTTACGCGCAGGTGACGAGGCGAGCGGCGAGAACGACGCGGCATGCCACCGTTGTCACGGTTATCAGCATTGTTCTCTTCTTGCTGTTCTGGTGCAg                                                                  <  2:432626/249‑1 (MQ=255)                                                                                                                                                                             gctCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGA‑GGCCAGTTCCGAAGAC‑GCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCACGATAGCGACGACGACGCTGACCACTTACGCGCAGGTGACGAGGCGAGCGGCGAGAACGACGCGGCATGCCACCGTTGTCCCGGTTATCAGCATTGTTCTCTTCTTGCTGTTCTGGTGCAGtt                                                                >  2:423224/1‑251 (MQ=255)                                                                                                                                                                             gctCTGCAACCTGTTCATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGA‑GGCCAGTTCCGAAGAC‑GCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCACGATAGCGACGACGACGCTGACCACTTACGCGCAGGTGACGAGGCGAGCGGCGAGAACGACGCGGCATGCCACCGTTGTCACGGTTATCAGCATTGTTCTCTTCTTGCTGTTCTGGTGCAGtt                                                                <  2:465325/251‑1 (MQ=255)                                                                                                                                                                                ctTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGA‑GGCCAGTTCCGAAGAC‑GCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCACGATAGCGACGACGACGCTGACCACTTACGCGCAGGTGACGAGGCGAGCGGCGAGAACGACGCGGCATGCCACCGTTGTCACGGTTATCAGCATTGTTCTCTTCTTGCTGTTCTGGTGCAGttt                                                               <  2:420970/249‑1 (MQ=255)                                                                                                                                                                                     aCCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGA‑GGCCAGTTCCGAAGAC‑GCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCACGATAGCGACGACGACGCTGACCACTTACGCGCAGGTGACGAGGCGAGCGGCGAGAACGACGCGGCATGCCACCGTTGTCACGGTTATCAGCATTGTTCTCTTCTTGCTGTTCTGGTGCAGTTTGCGCTAc                                                        <  2:462603/251‑1 (MQ=255)                                                                                                                                                                                      ccTGTCCATCTTGCGGACGTACCATTGGATAGCGGACCCAGACTTTGCCAGA‑GGCCAGTTCCGAAGAC‑GCGCACGCTACGGCCAACCGCATTGGCGACTGGGTTGGATAACGCTCGTCACGATAGCGCCGACGGCGCCGACCACTTACGCGCCGGCGACAAGGCGAGCGGCGACAACGCCGCGGCCTGCCACCGTTGTCACGGTTATCAGCCTTGTTCTCTTCTTGCTGTTCTGGCGCAGTTTgcgccacc                                                       >  2:221124/1‑247 (MQ=255)                                                                                                                                                                                               cTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGA‑GGCCAGTTCCGGATAC‑GCGCACGCTACGGTCAACGGCATGGGCGACTGGGTTGGATAACGCTCGTCACGATAGCGACGACGACGCTGACCACTTACGCGCAGGTGACGAGGCGGGAGGCGAGAACGACGCGGCGTGCCACCGTTGTCACGGTTATCAGCATTGTTCTCTTCTTGCTGTTCTGGTGCAGTTTGCTCTACGACTGGCAGc                                              >  1:174531/1‑251 (MQ=255)                                                                                                                                                                                                     gtcGTACAATTGGAAAGCGGATCCAGCCTTTGCCAGA‑GGCCAGTTCCGAAGAC‑GCGCACGCTGCGGGCAACGGCATTGGCGACTGGGTTGGTTAACGCTCGTCACGATAGCGACGACGCCGCTTACCTCTTTCGCGCAGGTGACGAGGCGAGCGGCGAGAACTACGCGGCATGCCACCTTTGTCACGGTTAGCAGCATTGTTCTCTTCTTGCTGTTCTTGTGCAGTTTGCGCTACGACTGGCAGCGGGACt                                        <  2:483332/249‑1 (MQ=255)                                                                                                                                                                                                     gACGTACAATTGGATAGCGGATCCAGACTTTGCCAGA‑GGCCAGTTCCGAAGAC‑GCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCACGATCGCGACGACGACGCTGACCACTTACGCGCAGGTGACGAGGCGTGCGGCGAGAACGCCGCGCCATTCCACCGTTGCCACGGTTATCAGCATTGTTCTTTTCTTGCTGTTCTGGTGCAGTTTGCGCTACGACtagacgcggggat                                        >  2:399921/1‑239 (MQ=255)                                                                                                                                                                                                        gTACAATTGGATAGCGGATCCAGACTTTGCCAGA‑GGCCAGTTCCGAAGAC‑GCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCACGATAGCGACGACGACGCTGACCACTTACGCGCAGGTGACGAGGCGAGCGGCGAGAACGACGCGGCATGCCACCGTTGTCACGGTTATCAGCATTGt                                                                                          <  1:316959/198‑1 (MQ=255)                                                                                                                                                                                                        gTACAATTGGATAGCGGATCCAGACTTTGCCAGA‑GGCCAGTTCCGAAGAC‑GCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCACGATAGCGACGACGACGCTGACCACTTACGCGCAGGAGACGAGGCGAGCGGCGAGAACGACGCGGCATGCCACCGTTGTCACGGTTATCAGCATTGt                                                                                          >  2:316959/1‑198 (MQ=255)                                                                                                                                                                                                                ggctAGCGGATCCAGACGTTGCCAGA‑GGCCAGTTCCGAAGAC‑GCGCACGCTACGGTCACCGGCATTGGCGACTGGGTTGGATAACGCTCGTCACGATAGCGACGACGACGCTGACCACTTACGCGCAGGTGACGAGGCGAGCGGCGAGAACGACGCGGCATGCCTCCGTTGTCACGGTTATCAGCATTGTTCTCTTCTTGCTGGTCTGGTGCAGTTTGCGCTACGACTGGCAGCGGGACTTTCACCAGTTc                             <  2:211049/248‑1 (MQ=255)                                                                                                                                                                                                                  aTAGCGGATCCAGACTTTGCCAGA‑GGCCAGTTCCGAAGAC‑GCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCACGATAGCGACGACGACGCTGACCACTTACGCGCAGGTGACGAGGCGAGCGGCGAGAACGACGCGGCATGCCACCGTTGTCACGGTTATCAGCATTGTTCTCTTCTTGCTGTTCTGGTGCAGTTTGCGCTACGACTGGCAGCGGGACTTTCACCAGTTCtg                           >  1:86176/1‑251 (MQ=255)                                                                                                                                                                                                                    aGCGGATCCAGACTTTGCCAGA‑GGCCAGTTCCGAAGAC‑GCGCCCGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCACGATAGCGACGACGACGCTGACCACTTACGCGCAGGTGACGAGGCGAGCGGCGAGAACGACGCGGCATGCCACCGTTGTCACGGTTATCAGCATTGTTCTCTTCTTGCTGTTCTGGTGCAGTTTGCGCTACGACTGGCAGCGGGACTTTCACCAGTTCtgtg                         <  2:419586/251‑1 (MQ=255)                                                                                                                                                                                                                    aGCGGATCCAGACTTTGCCAGA‑GGCCAGGTCCGAAGAC‑GCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCACGATAGCGACGACGACGCTGACCACTTACGCGCAGGTGACGAGGCGAGCGGCGAGAACGACGCGGCATGCCACCGTTGTCACGGTTATCAGCATTGTTCTCTTCTTGCTGTGCTGTTGCAGTTTGCGCTACGACTGGCAGCGGGACTTTCACCAGTTCtgt                          <  1:401094/250‑1 (MQ=255)                                                                                                                                                                                                                       ggATCCAGACTTTGCCAGA‑GGCCAGTTCCGAAGAC‑GCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCACGATAGCGACGACGACGCTGACCACTTACGCGCAGGTGACGAGGCGAGCGGCGAGAACGACGCGGCATGCCACCGTTGTCACGGTTATCAGCATTGTTCTCTTCTTGCTGTTCTGGTGCAGTTTGCGCTACGACTGGCAGCGGGACTTTCACCAGTTCTGTGCGt                      <  1:334168/251‑1 (MQ=255)                                                                                                                                                                                                                              gACTTTGCCAGA‑GGCCAGTTCCGAAGAC‑GCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCACGATAGCGACGACGACGCTGACCACTTACGCGCAGGTGACGAGGCGAGCGGCGAGAACGACGCGGCATGCCACCGTTGTCACGGTTATCAGCATTGTTCTCTTCTTGCTGTTCTGGTGCAGTTTGCGCTACGACTGGCAGCGGGACTTTCACCAGTTCTGTGCGTGGAGCTg               >  1:16221/1‑251 (MQ=255)                                                                                                                                                                                                                               aCTTTGCCAGA‑GGCCAGTTCCGAAGAC‑GCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCACGATAGCGACGCCGACGCTGACCACTTACGCGCAGGTGACGAGGCGAGCGGCGAGAACGACGCGGCATGCCACCGTTGTCACGGTTATCAGCATTGTTCTCTTCTTGCTGTTCTGGTGCCGTTTGCGCTACGACTGGCCGCGGGACTTTCCCCAGTTCTGTGCGTGGGGCTgg              >  2:444333/1‑251 (MQ=255)                                                                                                                                                                                                                               aCTTTGCCAGA‑GGCCAGTTCCGAAGAC‑GCGCACGCTACGGGCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCACGATAGCGACGACGACGCTGACCACTTACGCGCAGGTGACGAGGCGAGCGGCGAGAACGACGCGGCATGCCACCGTTGTCACGGTTATCAGCATTGTTCTCTTCTTGCTGTTCTGGTGCAGTTTGCGCTACGACTGGCAGCGGGACTTTCACCAGTTCTGTGCGTGGAGCTgg              <  2:86176/251‑1 (MQ=255)                                                                                                                                                                                                                                  ttGCCAGA‑GGCCAGTTCCGAAGAC‑GCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCACGATAGCGACGACGACGCTGACCACTTACGCGCAGGTGACGAGGCGAGCGGCGAGAACGACGCGGCATGCCACCGTTGTCACGGTTATCAGCATTGTTCTCTTCTTGCTGTTCTGGTGCAGTTTGCGCTACGACTGGCAGCGGGACTTTCACCAGTTCTGTGCGTGGAGCTGgc             <  1:63572/249‑1 (MQ=255)                                                                                                                                                                                                                                       aga‑gGCCAGGTCCGAAGAC‑GCGCACGCTACGGTCAACGGCATTGGCGACGGGGGTGGATAACGCTCGGCACGATAGCGACGACGACGCTGACCACTTACGCGCAGGTGACGAGGCGAGCGGCGAGAACGACGCGGCATGCCACCGTTGTCACGGTTATCAGCATTGTTCTCTTCTTGCGGTTCTGGTGCAGTTTGCGCTACGACTGGCAGCGGGACTTTCACCAGTTCTGTGCGTGGAGCTGGCGCTTCCt      <  2:483672/251‑1 (MQ=255)                                                                                                                                                                                                                                        ga‑gGCCAGTTCCGAAGAC‑GCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCACGATAGCGACGACGACGCTGACCACTTACGCGCAGGTGACGCGGCGAGCGGCGAGAACGACGCGGCATGCCACCGTTGTCACGGTTATCAGCATTGTTCTCTTCTTGCTGTTCTGGTGCAGTTTGCGCTACGCCTGGCAGCGGGACTTTCACCAGTTCTGTGCGTGGAGCTGGCGCTTCCtt     >  1:213791/1‑250 (MQ=255)                                                                                                                                                                                                                                         a‑gGCGAGTTTCGAAGAC‑TCGCACGGTTCGGTCAACGGCATTGGCGACGGGGTTGGATAACGCTCGTCACGATAGCGACGACGACGCTGACCACTTACGCGCAGGTGACGAGGCGAGCGGCGAGAACGACGCGGCATGCCACCGTTGTCACGGTTATCAGCATTGTTCTCTTCTTGCTGTTCTGGTGCAGTTTGCGCTACGACTGGCAGCGGGACTTTCACCAGTTCTGGGCGTGGAGCTGGCGCTTCCTGa    <  2:264028/251‑1 (MQ=255)                                                                                                                                                                                                                                         a‑gGCCAGTTCCGAAGAC‑GCGACCGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCACGATAGCGACGACGACGCTGACCACTTACGCGCAGGTGACGAGGCGAGCGGCGAGAACGACGCGGCATGCCACCGTTGTCACGGTTATCAGCATTGTTCTCTTCTTGCTGTTCTGGTGCAGTTTGCGCTACGACTGGCAGCGGGACTTTCACCAGTTCTGTGCGTGGAGCTGGCGCTTCCTGa    <  2:350152/251‑1 (MQ=255)                                                                                                                                                                                                                                            gCCAGTTCCGAAGAC‑GCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCACGATAGCGACGACGACGCTGACCACTTACGCGCAGGTGACGCGGCGAGCGGCGAGAACGACGCGGCATGCCACCGTTGTCACGGTTATCAGCATTGTTCTCTTCTTGCTGTTCTGGTGCAGTTTGCGCTACGACTGGCAGCGGGACTTTCACCAGTTCTGTGCGTGGAGCTGGCGCTTCCTGAAc  >  2:157436/1‑251 (MQ=255)                                                                                                                                                                                                                                            gCCAGTTCCGAAGAC‑GCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCACGATAGCGACGACGACGCTGACCACTTACGCGCAGGTGACGAGGCGAGCGGCGAGAACGACGCGGCATGCCACCGTTGTCACGGTTATCAGCATTGTTCTCTTCTTGCTGTTCTGGTGCAGTTTGCGCTACGACTGGCAGCGGGACTTTCACCCGTTCTGTGCGTGGAGCTGGCGCTTCCTGAAc  >  2:220033/1‑251 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                  TCAGGATGCGTCGTTTCAACCACTTCCGGTTGCGGTTCGGCAACCTGAACGGGGGCTTCTACGACACCGGCCACTTCTTCAACAACTGGCGCGCTAACAACCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCTGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGA‑GGCCAGTTCCGGAGAC‑GCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCACGATAGCGACGACGACGCTGACCACTTACGCGCAGGTGACGAGGCGAGCGGCGAGAACGACGCGGCATGCCACCGTTGTCACGGTTATCAGCATTGTTCTCTTCTTGCTGTTCTGGTGCAGTTTGCGCTACGACTGGCAGCGGGACTTTCACCAGTTCTGTGCGTGGAGCTGGCGCTTCCTGAAC  >  NC_000913/1141621‑1142105

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑